LOCUS       DAYYJC010000001        90658 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000001 DAYYJC010000000
VERSION     DAYYJC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 90658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.001"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA9N_000001"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACHA9N_000001"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9083503.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA9N_000002"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACHA9N_000002"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9083504.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA9N_000003"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACHA9N_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9083505.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA9N_000004"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACHA9N_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9083506.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA9N_000005"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACHA9N_000005"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9083507.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA9N_000006"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACHA9N_000006"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9083508.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACHA9N_000007"
     CDS             complement(8053..8523)
                     /locus_tag="ACHA9N_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9083509.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACHA9N_000008"
     CDS             complement(8569..8994)
                     /locus_tag="ACHA9N_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9083510.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACHA9N_000009"
     CDS             complement(9061..9330)
                     /locus_tag="ACHA9N_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9083511.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACHA9N_000010"
     CDS             complement(9341..10936)
                     /locus_tag="ACHA9N_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9083512.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA9N_000011"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACHA9N_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9083513.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA9N_000012"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACHA9N_000012"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9083514.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACHA9N_000013"
     CDS             15402..16376
                     /locus_tag="ACHA9N_000013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9083515.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACHA9N_000014"
     CDS             16546..17946
                     /locus_tag="ACHA9N_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9083516.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACHA9N_000015"
     CDS             complement(18024..18746)
                     /locus_tag="ACHA9N_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9083517.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACHA9N_000016"
     CDS             complement(18825..19331)
                     /locus_tag="ACHA9N_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9083518.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACHA9N_000017"
     CDS             19454..21253
                     /locus_tag="ACHA9N_000017"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9083519.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACHA9N_000018"
     CDS             complement(21321..22895)
                     /locus_tag="ACHA9N_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9083520.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHA9N_000019"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACHA9N_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9083521.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHA9N_000020"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACHA9N_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9083522.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACHA9N_000021"
     CDS             complement(24672..25310)
                     /locus_tag="ACHA9N_000021"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9083523.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHA9N_000022"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACHA9N_000022"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9083524.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHA9N_000023"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACHA9N_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9083525.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHA9N_000024"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACHA9N_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9083526.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACHA9N_000025"
     CDS             complement(29104..29844)
                     /locus_tag="ACHA9N_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9083527.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHA9N_000026"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACHA9N_000026"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9083528.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHA9N_000027"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACHA9N_000027"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9083529.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHA9N_000028"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACHA9N_000028"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9083530.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHA9N_000029"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACHA9N_000029"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9083531.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHA9N_000030"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACHA9N_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9083532.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHA9N_000031"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACHA9N_000031"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9083533.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACHA9N_000032"
     CDS             complement(36724..37008)
                     /locus_tag="ACHA9N_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083534.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACHA9N_000033"
     CDS             complement(37026..37916)
                     /locus_tag="ACHA9N_000033"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9083535.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACHA9N_000034"
     CDS             complement(37936..38154)
                     /locus_tag="ACHA9N_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9083536.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACHA9N_000035"
     CDS             complement(38124..39824)
                     /locus_tag="ACHA9N_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9083537.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACHA9N_000036"
     CDS             40091..40864
                     /locus_tag="ACHA9N_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083538.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHA9N_000037"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACHA9N_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9083539.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACHA9N_000038"
     CDS             complement(41688..42659)
                     /locus_tag="ACHA9N_000038"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9083540.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHA9N_000039"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACHA9N_000039"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9083541.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHA9N_000040"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACHA9N_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9083542.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ACHA9N_000041"
     CDS             complement(45614..46438)
                     /locus_tag="ACHA9N_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9083543.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ACHA9N_000042"
     CDS             complement(46411..47091)
                     /locus_tag="ACHA9N_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9083544.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ACHA9N_000043"
     CDS             complement(47114..48052)
                     /locus_tag="ACHA9N_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9083545.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACHA9N_000044"
     CDS             complement(48297..49148)
                     /gene="ispE"
                     /locus_tag="ACHA9N_000044"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9083546.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACHA9N_000045"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ACHA9N_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9083547.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ACHA9N_000046"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ACHA9N_000046"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9083548.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACHA9N_000047"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ACHA9N_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9083549.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACHA9N_000048"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ACHA9N_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9083550.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ACHA9N_000049"
     CDS             complement(56026..57267)
                     /locus_tag="ACHA9N_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9083551.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACHA9N_000050"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ACHA9N_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9083552.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ACHA9N_000051"
     CDS             complement(58294..59217)
                     /locus_tag="ACHA9N_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9083553.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ACHA9N_000052"
     CDS             complement(59956..61242)
                     /locus_tag="ACHA9N_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9083554.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ACHA9N_000053"
     CDS             complement(61235..62503)
                     /locus_tag="ACHA9N_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9083555.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="ACHA9N_000054"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="ACHA9N_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9083556.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="ACHA9N_000055"
     CDS             complement(63276..63722)
                     /locus_tag="ACHA9N_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083557.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="ACHA9N_000056"
     CDS             complement(63815..64060)
                     /locus_tag="ACHA9N_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9083558.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64175..64753)
                     /locus_tag="ACHA9N_000057"
     CDS             complement(64175..64753)
                     /locus_tag="ACHA9N_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9083559.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64830..65360)
                     /locus_tag="ACHA9N_000058"
     CDS             complement(64830..65360)
                     /locus_tag="ACHA9N_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9083560.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACHA9N_000059"
     CDS             complement(65364..66224)
                     /gene="rlmB"
                     /locus_tag="ACHA9N_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9083561.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66217..66612)
                     /locus_tag="ACHA9N_000060"
     CDS             complement(66217..66612)
                     /locus_tag="ACHA9N_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9083562.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACHA9N_000061"
     CDS             complement(66609..68021)
                     /gene="cysS"
                     /locus_tag="ACHA9N_000061"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9083563.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACHA9N_000062"
     CDS             complement(68018..68554)
                     /gene="epsC"
                     /locus_tag="ACHA9N_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9083564.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(68640..68879)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACHA9N_000063"
     CDS             complement(68876..70336)
                     /gene="gltX"
                     /locus_tag="ACHA9N_000063"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9083565.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70506..71663)
                     /locus_tag="ACHA9N_000064"
     CDS             complement(70506..71663)
                     /locus_tag="ACHA9N_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9083566.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACHA9N_000065"
     CDS             complement(71697..73067)
                     /gene="radA"
                     /locus_tag="ACHA9N_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9083567.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73130..73657)
                     /locus_tag="ACHA9N_000066"
     CDS             complement(73130..73657)
                     /locus_tag="ACHA9N_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9083568.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73887..74693
                     /gene="proB"
                     /locus_tag="ACHA9N_000067"
     CDS             73887..74693
                     /gene="proB"
                     /locus_tag="ACHA9N_000067"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9083569.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74690..75937
                     /locus_tag="ACHA9N_000068"
     CDS             74690..75937
                     /locus_tag="ACHA9N_000068"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9083570.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76013..76357
                     /locus_tag="ACHA9N_000069"
     CDS             76013..76357
                     /locus_tag="ACHA9N_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083571.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76474..76800)
                     /locus_tag="ACHA9N_000070"
     CDS             complement(76474..76800)
                     /locus_tag="ACHA9N_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083572.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77415..78617)
                     /locus_tag="ACHA9N_000071"
     CDS             complement(77415..78617)
                     /locus_tag="ACHA9N_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9083573.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78734..79249
                     /locus_tag="ACHA9N_000072"
     CDS             78734..79249
                     /locus_tag="ACHA9N_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9083574.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79448..79894)
                     /locus_tag="ACHA9N_000073"
     CDS             complement(79448..79894)
                     /locus_tag="ACHA9N_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9083575.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="ACHA9N_000074"
     CDS             complement(79941..81419)
                     /gene="msr(C)"
                     /locus_tag="ACHA9N_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9083576.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81773..82402)
                     /locus_tag="ACHA9N_000075"
     CDS             complement(81773..82402)
                     /locus_tag="ACHA9N_000075"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9083577.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82386..82850)
                     /locus_tag="ACHA9N_000076"
     CDS             complement(82386..82850)
                     /locus_tag="ACHA9N_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9083578.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83337..84605)
                     /locus_tag="ACHA9N_000077"
     CDS             complement(83337..84605)
                     /locus_tag="ACHA9N_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9083579.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84836..85573)
                     /locus_tag="ACHA9N_000078"
     CDS             complement(84836..85573)
                     /locus_tag="ACHA9N_000078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9083580.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85640..86209)
                     /locus_tag="ACHA9N_000079"
     CDS             complement(85640..86209)
                     /locus_tag="ACHA9N_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9083581.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86337..87209
                     /locus_tag="ACHA9N_000080"
     CDS             86337..87209
                     /locus_tag="ACHA9N_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9083582.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87551..88423
                     /locus_tag="ACHA9N_000081"
     CDS             87551..88423
                     /locus_tag="ACHA9N_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9083583.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88420..90378
                     /gene="nagE"
                     /locus_tag="ACHA9N_000082"
     CDS             88420..90378
                     /gene="nagE"
                     /locus_tag="ACHA9N_000082"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9083584.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
BASE COUNT        25286 a        19529 c        16369 g        29474 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaga aatgcaataa
    64141 aaataaataa gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt
    64201 gtttttttat tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg
    64261 ctaaacattc agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag
    64321 cttctatttt tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct
    64381 gaattgctaa aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta
    64441 ttttttggtc gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc
    64501 tcaacagaga atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac
    64561 ctttgctttt ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc
    64621 aatcctcaga aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa
    64681 aatatttttg atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata
    64741 actcctcaga cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa
    64801 tgattcatcg aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat
    64861 ttttgggaca atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata
    64921 aaatgcatat ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat
    64981 aattcataag cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa
    65041 tcgcttgtcg ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg
    65101 atataactgt cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta
    65161 taattttgtt ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct
    65221 tcatatttcg cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg
    65281 tcctgctttt taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca
    65341 atcaacagct gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata
    65401 agcaaaccag cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc
    65461 agcaattcat ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg
    65521 actaaagcaa ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg
    65581 ccaaataccc aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg
    65641 cgtgcaacag ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt
    65701 ccaactgctc ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt
    65761 aaaatcgacc caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct
    65821 tcttttttct cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta
    65881 atcgcaagaa cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta
    65941 ggaacccatt tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt
    66001 tctcctcgtg aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct
    66061 actgcggcat ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa
    66121 actgactgat ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca
    66181 aatccacgtt tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt
    66241 ctgaatacac cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata
    66301 gcccatgagc gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc
    66361 gctcgtatgg cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa
    66421 aagatgttct tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt
    66481 cgttgccgca cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg
    66541 aacatagatt tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt
    66601 gtaatctctc atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc
    66661 cctttttctt tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt
    66721 gcttgatttc gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta
    66781 aataaaatac cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca
    66841 gcggcagaaa ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc
    66901 actgtgatcc catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct
    66961 aaattttcta acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct
    67021 tggcggaatt tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct
    67081 ttcatcgtcg tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa
    67141 cgcaagactt gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga
    67201 gatttactca ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa
    67261 ttagcgaatt ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga
    67321 aattctaaat cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc
    67381 ataacggaac attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa
    67441 atctcgccag gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc
    67501 tgttcttccc ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt
    67561 aatttcccat aattttcaaa tttacgggta cggtaataca catcacctga agattcataa
    67621 gcatagcctt tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg
    67681 actcttggat gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct
    67741 cggatgaaac gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg
    67801 attttatcgt ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca
    67861 aaatagcgtc gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa
    67921 ttatacacag tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat
    67981 tcttcttttt ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt
    68041 ctcctttgat tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat
    68101 ctttcaacac aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta
    68161 atatctgcgc attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg
    68221 tttcttttcc tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt
    68281 ctgcggtttc gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg
    68341 ttgctcctgg atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg
    68401 ccaataaaaa gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat
    68461 gaaaaccagg gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct
    68521 ttgctgcatc aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt
    68581 ttatgtagga aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat
    68641 caaaagacgc tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc
    68701 taacgcctgt tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc
    68761 tgtgtttcgc caagaccact cccagaggca tttcggcaac cattcattga taccttgcac
    68821 cacccggtat ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt
    68881 tcagtacttg atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag
    68941 gcaattctgg accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt
    69001 ttccttttac gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat
    69061 ctactacttc catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt
    69121 caccagcaag tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg
    69181 tagacagatc aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct
    69241 tcttcaccca ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta
    69301 gataaggcag acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt
    69361 ttacccattg cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat
    69421 cgaacatttt gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc
    69481 ccaataatgc aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt
    69541 cgataaattg caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga
    69601 tgatcaatgt catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca
    69661 gttgttttgg tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca
    69721 aatgatcatc gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga
    69781 caaagtcgcc accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa
    69841 atgcgtattc tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt
    69901 ctttttctgc ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt
    69961 ctcctcttgc gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt
    70021 tgtatgctcg attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt
    70081 cagattgacg gtaagggccg tattctccag gattttcagg agattcatcc caattcattc
    70141 ccaaccaagc aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt
    70201 cagtatcctc aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga
    70261 ataatgcagt tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta
    70321 cgcgtacttt cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa
    70381 ttccgtcagt tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac
    70441 cttttctaag gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt
    70501 cttttttatt ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt
    70561 cctgcatgtg caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt
    70621 acaacgactt cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg
    70681 ccgtcatcaa ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca
    70741 ttcatcgttt caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta
    70801 agaacaggta cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg
    70861 acaccatcta aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca
    70921 aagtctccat cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata
    70981 tctaatccgc gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt
    71041 agttcgtaaa gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta
    71101 atatcataaa tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa
    71161 ttttctcctg attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg
    71221 atttttttgc ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt
    71281 ctcaatccca gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat
    71341 ggaatttcca tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc
    71401 gcaccgatcg caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact
    71461 tgtttgataa tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt
    71521 gcgcctaaca ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt
    71581 gcctgccaag ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc
    71641 aaagtaatga cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag
    71701 cggaacacac gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt
    71761 ggcgcttccc agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt
    71821 tggacttctt tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca
    71881 atttcaccga tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata
    71941 ctgacagcga tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt
    72001 aagtaagcat cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc
    72061 agagataccc gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta
    72121 ggtgtgacta atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg
    72181 atagcagagc cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg
    72241 acttcttcca atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat
    72301 cggtttttta ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac
    72361 accgtatcca ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca
    72421 tgaccgacga taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg
    72481 gtttctctta cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt
    72541 tggatggaat caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg
    72601 ttcatatctg tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg
    72661 cgtaatttta tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg
    72721 gctgctaatt gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca
    72781 ccaatcaaga ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct
    72841 aattttgttt tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt
    72901 gctttttctc ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct
    72961 tcgaccatcg tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca
    73021 tacccgcaat tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct
    73081 tttatcctaa tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg
    73141 aagaaccaaa tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg
    73201 gcaaaaagat cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa
    73261 agttggtgaa ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt
    73321 caatcacacc tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt
    73381 tagcaagctg caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg
    73441 ttggtttcaa ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt
    73501 ccaacccctt tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt
    73561 caaaatcata gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt
    73621 tatatttaga aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact
    73681 actcctattc taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat
    73741 tctgctatct taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca
    73801 aaaaaactat tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat
    73861 gagtggatat ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta
    73921 ttgtcaaagt aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa
    73981 tcgatcagtt agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag
    74041 tctcatcagg agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt
    74101 tgattcctga acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata
    74161 atcaaagatt cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg
    74221 tagattatcc tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg
    74281 acacgatccc tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga
    74341 caaaatttgg cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc
    74401 ttttgatcgt cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg
    74461 aggctcagat gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg
    74521 gtgctggtag cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga
    74581 tatttgaagc aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg
    74641 atattcttgc aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt
    74701 gatgcaatta ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa
    74761 ccgcaaaaat gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat
    74821 cattgaagca aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat
    74881 gcaagaccgc ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa
    74941 aattgcttcg ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg
    75001 attgagaatc ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg
    75061 tccaaatgtc acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct
    75121 tcgcggtggc aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt
    75181 actggtcagt gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga
    75241 aactgcagga cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg
    75301 agcaaaatta atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac
    75361 aggaaataat cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat
    75421 cgtcaatgca aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca
    75481 ttctgagatc gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt
    75541 ctctttacgt gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga
    75601 cacagattgg gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt
    75661 ggatgaagca attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac
    75721 agatagttat gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt
    75781 caatgcctcc actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat
    75841 ctctactcaa aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa
    75901 atatatcatt tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc
    75961 cgccttttgc cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga
    76021 aattttaatg atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt
    76081 cggtatttta tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa
    76141 atggtcgcag tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag
    76201 ttacatggtt gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc
    76261 aatcgcttat acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca
    76321 gctgaataaa aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga
    76381 gtgccttgaa gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt
    76441 cacagcctct ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc
    76501 ctcttcgtta agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc
    76561 atccatggac aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg
    76621 ttcccgatac acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc
    76681 ttccgcattg attattggca attctttgat atagccacga taaatcaacc gatcatctga
    76741 aaattcaatg ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat
    76801 ctattatccc cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca
    76861 gtttcattat tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc
    76921 cgaataaatg gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat
    76981 gagaaactgt tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc
    77041 tcttggtaaa cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata
    77101 tgagaagcta tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa
    77161 cctctgttaa acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat
    77221 atgaggaact atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa
    77281 cctcctgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    77341 aatatgagaa gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca
    77401 caacctctga tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata
    77461 caacatacac tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata
    77521 tgttcaccaa tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa
    77581 ctatagctgg acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc
    77641 aacagtgtat tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg
    77701 actggcatag aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct
    77761 gctaaaattg tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa
    77821 gcaatagcca gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag
    77881 ccaatcaata aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat
    77941 gcgattagtc caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg
    78001 cctacttttc tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg
    78061 caaaacatca aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc
    78121 ccaccgacta gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt
    78181 gctttttctg tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat
    78241 aaagcattcc cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat
    78301 aatccgccaa gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc
    78361 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
    78421 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
    78481 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
    78541 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
    78601 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
    78661 caatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
    78721 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
    78781 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
    78841 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
    78901 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
    78961 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
    79021 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
    79081 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
    79141 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
    79201 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
    79261 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
    79321 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
    79381 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
    79441 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
    79501 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
    79561 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
    79621 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
    79681 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
    79741 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
    79801 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
    79861 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
    79921 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
    79981 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
    80041 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
    80101 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
    80161 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
    80221 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
    80281 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
    80341 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
    80401 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
    80461 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
    80521 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
    80581 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
    80641 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
    80701 agtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
    80761 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
    80821 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
    80881 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
    80941 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
    81001 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
    81061 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
    81121 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
    81181 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
    81241 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
    81301 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
    81361 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
    81421 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
    81481 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
    81541 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
    81601 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
    81661 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
    81721 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
    81781 acatagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
    81841 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
    81901 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
    81961 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
    82021 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
    82081 gcatcttccc tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca
    82141 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
    82201 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
    82261 tcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
    82321 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
    82381 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
    82441 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
    82501 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc
    82561 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
    82621 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
    82681 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
    82741 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
    82801 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
    82861 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
    82921 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
    82981 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
    83041 attgattctt atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca
    83101 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
    83161 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
    83221 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
    83281 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
    83341 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
    83401 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
    83461 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
    83521 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
    83581 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
    83641 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
    83701 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
    83761 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
    83821 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
    83881 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    83941 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    84001 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    84061 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    84121 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    84181 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    84241 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    84301 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    84361 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    84421 attgtatttt ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct
    84481 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    84541 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    84601 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    84661 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt
    84721 tttctgcata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    84781 gtcgtttgaa taactattta acaagtaatt ctctctggat attatccgtt atggattata
    84841 cttcctgtgt cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta
    84901 tcgcaaatgc tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt
    84961 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    85021 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    85081 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    85141 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    85201 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    85261 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    85321 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    85381 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    85441 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    85501 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    85561 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    85621 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    85681 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    85741 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    85801 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    85861 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    85921 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    85981 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    86041 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    86101 ctgctaaagg ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg
    86161 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    86221 cttttatcct tactttataa cggacggctt atgatgtcta atacttataa aacatactta
    86281 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    86341 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    86401 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    86461 aacttggtgt gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc
    86521 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    86581 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    86641 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    86701 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    86761 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    86821 aagatcgttg gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc
    86881 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    86941 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    87001 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    87061 ggatcatcaa tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag
    87121 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    87181 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    87241 taacaacgaa taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatttg
    87301 ttccttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    87361 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    87421 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    87481 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    87541 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    87601 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    87661 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    87721 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    87781 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    87841 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    87901 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    87961 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    88021 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    88081 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    88141 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    88201 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    88261 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    88321 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    88381 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    88441 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    88501 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    88561 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    88621 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    88681 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    88741 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    88801 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    88861 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    88921 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    88981 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    89041 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    89101 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    89161 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    89221 aacaaagcaa aaacagcttc cttgatgtta gcagcagctt ttgcttcatt ctttactggt
    89281 gtaacagaac cacttgaatt ctcatttatg ttcgttgctt ggccattata tgtcttgcat
    89341 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    89401 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    89461 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    89521 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    89581 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    89641 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    89701 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    89761 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    89821 gttgcagatg agatggcaaa actacatggt ggtgctgcaa cacgacctgc acaaacaaac
    89881 accgtggtta aaacagaaac atttgccggt ggaacgatcg aacaagatat ttacgctatc
    89941 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    90001 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    90061 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    90121 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    90181 tatgtaaaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    90241 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    90301 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    90361 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    90421 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    90481 aactccgcgg taataagtcc agccaaacgt aacatatgaa gagctatttt ttatttgact
    90541 cttcttatta cttgatgcag aacagctttt ttacaacctc tgataaacgg tgttcaaaaa
    90601 ccagtttatc ggtatgaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaa
//
LOCUS       DAYYJC010000002        84310 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000002 DAYYJC010000000
VERSION     DAYYJC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.002"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..973
                     /locus_tag="ACHA9N_000083"
     CDS             281..973
                     /locus_tag="ACHA9N_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9083585.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="ACHA9N_000084"
     CDS             1181..1471
                     /locus_tag="ACHA9N_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083586.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1743..2092)
                     /locus_tag="ACHA9N_000085"
                     /pseudo
     CDS             complement(1743..2092)
                     /locus_tag="ACHA9N_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            2185..3555
                     /locus_tag="ACHA9N_000086"
     CDS             2185..3555
                     /locus_tag="ACHA9N_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9083587.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="ACHA9N_000087"
     tRNA            complement(3795..3878)
                     /locus_tag="ACHA9N_000087"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            3951..4112
                     /locus_tag="ACHA9N_000088"
     CDS             3951..4112
                     /locus_tag="ACHA9N_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083588.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            4261..5478
                     /locus_tag="ACHA9N_000089"
     CDS             4261..5478
                     /locus_tag="ACHA9N_000089"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9083589.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="ACHA9N_000090"
     CDS             5662..6498
                     /locus_tag="ACHA9N_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9083590.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="ACHA9N_000091"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="ACHA9N_000091"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9083591.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7999..9000
                     /locus_tag="ACHA9N_000092"
     CDS             7999..9000
                     /locus_tag="ACHA9N_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9083592.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            9019..10038
                     /locus_tag="ACHA9N_000093"
     CDS             9019..10038
                     /locus_tag="ACHA9N_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9083593.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(10140..10856)
                     /locus_tag="ACHA9N_000094"
     CDS             complement(10140..10856)
                     /locus_tag="ACHA9N_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9083594.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="ACHA9N_000095"
     CDS             complement(10825..11610)
                     /locus_tag="ACHA9N_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9083595.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="ACHA9N_000096"
     CDS             complement(11601..12350)
                     /locus_tag="ACHA9N_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9083596.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="ACHA9N_000097"
     CDS             12648..13172
                     /locus_tag="ACHA9N_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9083597.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="ACHA9N_000098"
     CDS             13188..14687
                     /locus_tag="ACHA9N_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9083598.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="ACHA9N_000099"
     CDS             complement(14775..15377)
                     /locus_tag="ACHA9N_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9083599.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="ACHA9N_000100"
     CDS             15481..16437
                     /locus_tag="ACHA9N_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9083600.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="ACHA9N_000101"
     CDS             complement(16742..17698)
                     /locus_tag="ACHA9N_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9083601.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="ACHA9N_000102"
     CDS             17872..18432
                     /locus_tag="ACHA9N_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083602.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="ACHA9N_000103"
     CDS             18425..19249
                     /locus_tag="ACHA9N_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9083603.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="ACHA9N_000104"
     CDS             19259..19876
                     /locus_tag="ACHA9N_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9083604.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="ACHA9N_000105"
     CDS             19894..20577
                     /locus_tag="ACHA9N_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9083605.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="ACHA9N_000106"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="ACHA9N_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            22503..23390
                     /locus_tag="ACHA9N_000107"
     CDS             22503..23390
                     /locus_tag="ACHA9N_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9083606.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="ACHA9N_000108"
     CDS             23407..24243
                     /locus_tag="ACHA9N_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9083607.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="ACHA9N_000109"
     CDS             24246..24869
                     /locus_tag="ACHA9N_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083608.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="ACHA9N_000110"
     CDS             24866..26158
                     /locus_tag="ACHA9N_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9083609.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="ACHA9N_000111"
     CDS             26730..27311
                     /locus_tag="ACHA9N_000111"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9083610.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="ACHA9N_000112"
     CDS             27314..28621
                     /locus_tag="ACHA9N_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9083611.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="ACHA9N_000113"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="ACHA9N_000113"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9083612.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29395..30519
                     /gene="purK"
                     /locus_tag="ACHA9N_000114"
     CDS             29395..30519
                     /gene="purK"
                     /locus_tag="ACHA9N_000114"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9083613.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="ACHA9N_000115"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="ACHA9N_000115"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9083614.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="ACHA9N_000116"
     CDS             complement(32007..32384)
                     /locus_tag="ACHA9N_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083615.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /gene="purC"
                     /locus_tag="ACHA9N_000117"
     CDS             32640..33368
                     /gene="purC"
                     /locus_tag="ACHA9N_000117"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9083616.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="ACHA9N_000118"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="ACHA9N_000118"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9083617.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="ACHA9N_000119"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="ACHA9N_000119"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9083618.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="ACHA9N_000120"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="ACHA9N_000120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9083619.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /gene="purF"
                     /locus_tag="ACHA9N_000121"
     CDS             36503..37942
                     /gene="purF"
                     /locus_tag="ACHA9N_000121"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9083620.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37965..39017
                     /gene="purM"
                     /locus_tag="ACHA9N_000122"
     CDS             37965..39017
                     /gene="purM"
                     /locus_tag="ACHA9N_000122"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9083621.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="ACHA9N_000123"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="ACHA9N_000123"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9083622.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="ACHA9N_000124"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="ACHA9N_000124"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9083623.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="ACHA9N_000125"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="ACHA9N_000125"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9083624.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="ACHA9N_000126"
     CDS             42536..43450
                     /locus_tag="ACHA9N_000126"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9083625.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="ACHA9N_000127"
     CDS             complement(43583..45148)
                     /locus_tag="ACHA9N_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9083626.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="ACHA9N_000128"
     CDS             complement(45141..46592)
                     /locus_tag="ACHA9N_000128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9083627.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="ACHA9N_000129"
     CDS             complement(46606..47622)
                     /locus_tag="ACHA9N_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9083628.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /gene="chvE"
                     /locus_tag="ACHA9N_000130"
     CDS             47842..48930
                     /gene="chvE"
                     /locus_tag="ACHA9N_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9083629.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /gene="mmsA"
                     /locus_tag="ACHA9N_000131"
     CDS             48984..50510
                     /gene="mmsA"
                     /locus_tag="ACHA9N_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9083630.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /gene="mmsB"
                     /locus_tag="ACHA9N_000132"
     CDS             50512..51717
                     /gene="mmsB"
                     /locus_tag="ACHA9N_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9083631.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="ACHA9N_000133"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="ACHA9N_000133"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9083632.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="ACHA9N_000134"
     CDS             53215..54639
                     /locus_tag="ACHA9N_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9083633.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(54681..54938)
                     /locus_tag="ACHA9N_000135"
     CDS             complement(54681..54938)
                     /locus_tag="ACHA9N_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083634.1"
                     /translation="MPIRILTALVNKITIRKAFYFSSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="ACHA9N_000136"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="ACHA9N_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9083635.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="ACHA9N_000137"
     CDS             56212..57189
                     /locus_tag="ACHA9N_000137"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9083636.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            57214..58857
                     /locus_tag="ACHA9N_000138"
     CDS             57214..58857
                     /locus_tag="ACHA9N_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM9083637.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            58865..60271
                     /gene="lpdA"
                     /locus_tag="ACHA9N_000139"
     CDS             58865..60271
                     /gene="lpdA"
                     /locus_tag="ACHA9N_000139"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9083638.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            60433..60708
                     /locus_tag="ACHA9N_000140"
     CDS             60433..60708
                     /locus_tag="ACHA9N_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9083639.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            60714..61481
                     /locus_tag="ACHA9N_000141"
     CDS             60714..61481
                     /locus_tag="ACHA9N_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9083640.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            61649..63478
                     /gene="typA"
                     /locus_tag="ACHA9N_000142"
     CDS             61649..63478
                     /gene="typA"
                     /locus_tag="ACHA9N_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9083641.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            63739..65511
                     /gene="recQ"
                     /locus_tag="ACHA9N_000143"
     CDS             63739..65511
                     /gene="recQ"
                     /locus_tag="ACHA9N_000143"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9083642.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            65625..65927
                     /locus_tag="ACHA9N_000144"
     CDS             65625..65927
                     /locus_tag="ACHA9N_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9083643.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            65930..67093
                     /locus_tag="ACHA9N_000145"
     CDS             65930..67093
                     /locus_tag="ACHA9N_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9083644.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            67119..70547
                     /locus_tag="ACHA9N_000146"
     CDS             67119..70547
                     /locus_tag="ACHA9N_000146"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9083645.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            70568..71692
                     /locus_tag="ACHA9N_000147"
     CDS             70568..71692
                     /locus_tag="ACHA9N_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9083646.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            71673..72119
                     /locus_tag="ACHA9N_000148"
     CDS             71673..72119
                     /locus_tag="ACHA9N_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9083647.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            72103..72441
                     /locus_tag="ACHA9N_000149"
     CDS             72103..72441
                     /locus_tag="ACHA9N_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9083648.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            72560..73120
                     /gene="rsmD"
                     /locus_tag="ACHA9N_000150"
     CDS             72560..73120
                     /gene="rsmD"
                     /locus_tag="ACHA9N_000150"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9083649.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            73117..73608
                     /gene="coaD"
                     /locus_tag="ACHA9N_000151"
     CDS             73117..73608
                     /gene="coaD"
                     /locus_tag="ACHA9N_000151"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9083650.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            73598..74641
                     /locus_tag="ACHA9N_000152"
     CDS             73598..74641
                     /locus_tag="ACHA9N_000152"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9083651.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            74800..75528
                     /locus_tag="ACHA9N_000153"
     CDS             74800..75528
                     /locus_tag="ACHA9N_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9083652.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            75644..75889
                     /locus_tag="ACHA9N_000154"
     CDS             75644..75889
                     /locus_tag="ACHA9N_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9083653.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            75886..76104
                     /locus_tag="ACHA9N_000155"
     CDS             75886..76104
                     /locus_tag="ACHA9N_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9083654.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            76240..76779
                     /locus_tag="ACHA9N_000156"
     CDS             76240..76779
                     /locus_tag="ACHA9N_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9083655.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            76772..79045
                     /locus_tag="ACHA9N_000157"
     CDS             76772..79045
                     /locus_tag="ACHA9N_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9083656.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            79083..80129
                     /gene="holA"
                     /locus_tag="ACHA9N_000158"
     CDS             79083..80129
                     /gene="holA"
                     /locus_tag="ACHA9N_000158"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9083657.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(80218..80472)
                     /gene="rpsT"
                     /locus_tag="ACHA9N_000159"
     CDS             complement(80218..80472)
                     /gene="rpsT"
                     /locus_tag="ACHA9N_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9083658.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(80872..81141)
                     /locus_tag="ACHA9N_000160"
     CDS             complement(80872..81141)
                     /locus_tag="ACHA9N_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083659.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(81403..81531)
                     /locus_tag="ACHA9N_000161"
     CDS             complement(81403..81531)
                     /locus_tag="ACHA9N_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083660.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(81611..82066)
                     /locus_tag="ACHA9N_000162"
     CDS             complement(81611..82066)
                     /locus_tag="ACHA9N_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9083661.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(82204..84132)
                     /locus_tag="ACHA9N_000163"
     CDS             complement(82204..84132)
                     /locus_tag="ACHA9N_000163"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9083662.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        27483 a        14397 c        18141 g        24289 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    57241 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    57301 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg
    57361 tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt
    57421 ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt
    57481 gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa
    57541 tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga
    57601 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    57661 gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca
    57721 gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta
    57781 gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa
    57841 gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca
    57901 aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat
    57961 atctcacaag taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac
    58021 ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca
    58081 ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag
    58141 tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct
    58201 aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa
    58261 gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg
    58321 aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca
    58381 gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat
    58441 atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac
    58501 cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac
    58561 ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct
    58621 gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta
    58681 ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg
    58741 atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca
    58801 atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa
    58861 acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc
    58921 tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga
    58981 aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat
    59041 agctgcagga caccattatc aagagtcact agattctgaa atgtttggag taactagtga
    59101 aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac
    59161 gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga
    59221 agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta
    59281 ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa
    59341 attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa
    59401 atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt
    59461 aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga
    59521 tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc
    59581 aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg
    59641 gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac
    59701 agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt
    59761 ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg
    59821 ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg
    59881 caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt
    59941 ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata
    60001 taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat
    60061 ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg
    60121 cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga
    60181 tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga
    60241 attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga
    60301 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
    60361 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
    60421 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
    60481 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
    60541 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
    60601 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
    60661 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
    60721 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
    60781 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
    60841 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
    60901 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
    60961 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
    61021 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
    61081 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
    61141 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
    61201 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
    61261 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
    61321 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
    61381 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
    61441 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
    61501 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
    61561 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
    61621 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
    61681 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
    61741 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
    61801 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
    61861 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
    61921 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
    61981 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
    62041 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
    62101 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
    62161 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
    62221 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
    62281 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
    62341 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
    62401 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
    62461 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
    62521 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
    62581 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
    62641 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
    62701 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
    62761 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
    62821 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
    62881 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
    62941 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
    63001 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
    63061 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
    63121 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
    63181 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
    63241 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
    63301 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
    63361 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
    63421 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
    63481 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
    63541 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
    63601 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
    63661 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
    63721 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggtta tgatgagttt
    63781 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
    63841 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
    63901 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
    63961 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
    64021 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
    64081 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
    64141 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
    64201 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
    64261 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
    64321 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
    64381 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
    64441 agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga
    64501 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
    64561 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
    64621 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
    64681 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
    64741 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
    64801 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
    64861 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
    64921 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
    64981 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
    65041 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
    65101 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
    65161 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
    65221 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
    65281 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
    65341 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
    65401 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
    65461 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
    65521 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
    65581 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
    65641 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
    65701 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
    65761 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
    65821 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
    65881 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
    65941 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
    66001 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
    66061 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
    66121 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
    66181 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
    66241 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
    66301 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
    66361 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
    66421 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
    66481 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
    66541 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
    66601 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
    66661 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
    66721 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
    66781 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
    66841 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
    66901 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg
    66961 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
    67021 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
    67081 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
    67141 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
    67201 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
    67261 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
    67321 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
    67381 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
    67441 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
    67501 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
    67561 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
    67621 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
    67681 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
    67741 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
    67801 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
    67861 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
    67921 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
    67981 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
    68041 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
    68101 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
    68161 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
    68221 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
    68281 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
    68341 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
    68401 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
    68461 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
    68521 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
    68581 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
    68641 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
    68701 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
    68761 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
    68821 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
    68881 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
    68941 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
    69001 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
    69061 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
    69121 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
    69181 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
    69241 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
    69301 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
    69361 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
    69421 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
    69481 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
    69541 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
    69601 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
    69661 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
    69721 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
    69781 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
    69841 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
    69901 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
    69961 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
    70021 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
    70081 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
    70141 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
    70201 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
    70261 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    70321 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    70381 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    70441 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    70501 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    70561 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    70621 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    70681 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    70741 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    70801 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    70861 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    70921 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    70981 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    71041 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    71101 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    71161 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    71221 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    71281 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    71341 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    71401 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    71461 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    71521 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    71581 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    71641 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    71701 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    71761 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    71821 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    71881 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    71941 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    72001 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    72061 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    72121 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    72181 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    72241 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    72301 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    72361 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    72421 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    72481 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    72541 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    72601 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    72661 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    72721 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    72781 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    72841 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    72901 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    72961 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    73021 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    73081 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    73141 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    73201 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    73261 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    73321 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    73381 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    73441 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    73501 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    73561 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    73621 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    73681 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    73741 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    73801 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    73861 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    73921 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    73981 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    74041 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    74101 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    74161 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    74221 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    74281 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    74341 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    74401 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    74461 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    74521 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    74581 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    74641 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    74701 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    74761 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    74821 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    74881 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    74941 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    75001 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    75061 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    75121 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    75181 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    75241 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    75301 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    75361 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    75421 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    75481 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    75541 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    75601 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    75661 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    75721 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    75781 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    75841 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    75901 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    75961 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    76021 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    76081 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    76141 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    76201 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    76261 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    76321 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    76381 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    76441 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    76501 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    76561 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    76621 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    76681 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    76741 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    76801 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    76861 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    76921 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    76981 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    77041 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    77101 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    77161 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    77221 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    77281 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    77341 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    77401 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    77461 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    77521 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    77581 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    77641 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    77701 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    77761 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    77821 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    77881 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    77941 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    78001 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    78061 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    78121 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    78181 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    78241 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    78301 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    78361 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    78421 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    78481 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    78541 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    78601 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    78661 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    78721 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    78781 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    78841 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    78901 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    78961 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    79021 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    79081 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    79141 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    79201 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    79261 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    79321 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    79381 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    79441 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    79501 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    79561 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    79621 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    79681 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    79741 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    79801 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    79861 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    79921 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    79981 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    80041 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    80101 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    80161 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    80221 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    80281 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    80341 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    80401 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    80461 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    80521 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    80581 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    80641 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    80701 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    80761 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    80821 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    80881 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    80941 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    81001 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    81061 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    81121 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    81181 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    81241 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    81301 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    81361 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    81421 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    81481 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    81541 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    81601 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    81661 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    81721 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    81781 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    81841 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    81901 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    81961 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    82021 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    82081 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct
    82141 tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt
    82201 tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga
    82261 agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat
    82321 tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga
    82381 agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg
    82441 catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac
    82501 gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc
    82561 ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag
    82621 ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg
    82681 ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat
    82741 acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg
    82801 aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt
    82861 tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt
    82921 ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa
    82981 tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc
    83041 gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag
    83101 aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc
    83161 tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa
    83221 atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga
    83281 agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc
    83341 tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag
    83401 aggaacagca agcaaaatca attttacata attattttcg attcgttcga taaatgaagc
    83461 tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt
    83521 ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc
    83581 atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc
    83641 atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc
    83701 gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt
    83761 ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg
    83821 acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat
    83881 tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa
    83941 atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata
    84001 tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc
    84061 gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt
    84121 ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta
    84181 attgataata ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg
    84241 atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc
    84301 tcaaataaga
//
LOCUS       DAYYJC010000003        75467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000003 DAYYJC010000000
VERSION     DAYYJC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.003"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..1673
                     /locus_tag="ACHA9N_000164"
     CDS             42..1673
                     /locus_tag="ACHA9N_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9083663.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1735..2376)
                     /locus_tag="ACHA9N_000165"
     CDS             complement(1735..2376)
                     /locus_tag="ACHA9N_000165"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9083664.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2562..2638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2658..2966
                     /gene="rplU"
                     /locus_tag="ACHA9N_000166"
     CDS             2658..2966
                     /gene="rplU"
                     /locus_tag="ACHA9N_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9083665.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2993..3337
                     /locus_tag="ACHA9N_000167"
     CDS             2993..3337
                     /locus_tag="ACHA9N_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9083666.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3350..3643
                     /gene="rpmA"
                     /locus_tag="ACHA9N_000168"
     CDS             3350..3643
                     /gene="rpmA"
                     /locus_tag="ACHA9N_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9083667.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4077..6884
                     /locus_tag="ACHA9N_000169"
     CDS             4077..6884
                     /locus_tag="ACHA9N_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9083668.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7075..7566
                     /locus_tag="ACHA9N_000170"
     CDS             7075..7566
                     /locus_tag="ACHA9N_000170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9083669.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7669..8658
                     /locus_tag="ACHA9N_000171"
     CDS             7669..8658
                     /locus_tag="ACHA9N_000171"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9083670.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8691..9494
                     /locus_tag="ACHA9N_000172"
     CDS             8691..9494
                     /locus_tag="ACHA9N_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9083671.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9515..10429
                     /locus_tag="ACHA9N_000173"
     CDS             9515..10429
                     /locus_tag="ACHA9N_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9083672.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10512..10886
                     /locus_tag="ACHA9N_000174"
     CDS             10512..10886
                     /locus_tag="ACHA9N_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9083673.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11025..11270
                     /locus_tag="ACHA9N_000175"
     CDS             11025..11270
                     /locus_tag="ACHA9N_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9083674.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11442..12506
                     /locus_tag="ACHA9N_000176"
     CDS             11442..12506
                     /locus_tag="ACHA9N_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9083675.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12752..13177
                     /locus_tag="ACHA9N_000177"
     CDS             12752..13177
                     /locus_tag="ACHA9N_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9083676.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13170..13619
                     /gene="nusB"
                     /locus_tag="ACHA9N_000178"
     CDS             13170..13619
                     /gene="nusB"
                     /locus_tag="ACHA9N_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9083677.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13716..14570
                     /locus_tag="ACHA9N_000179"
     CDS             13716..14570
                     /locus_tag="ACHA9N_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9083678.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14571..15908
                     /gene="xseA"
                     /locus_tag="ACHA9N_000180"
     CDS             14571..15908
                     /gene="xseA"
                     /locus_tag="ACHA9N_000180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9083679.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15913..16137
                     /locus_tag="ACHA9N_000181"
     CDS             15913..16137
                     /locus_tag="ACHA9N_000181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9083680.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16139..17029
                     /locus_tag="ACHA9N_000182"
     CDS             16139..17029
                     /locus_tag="ACHA9N_000182"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9083681.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17038..17856
                     /locus_tag="ACHA9N_000183"
     CDS             17038..17856
                     /locus_tag="ACHA9N_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9083682.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17963..18412
                     /locus_tag="ACHA9N_000184"
     CDS             17963..18412
                     /locus_tag="ACHA9N_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9083683.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18430..20112
                     /gene="recN"
                     /locus_tag="ACHA9N_000185"
     CDS             18430..20112
                     /gene="recN"
                     /locus_tag="ACHA9N_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9083684.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20167..20283)
                     /locus_tag="ACHA9N_000186"
     CDS             complement(20167..20283)
                     /locus_tag="ACHA9N_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9083685.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20356..21312)
                     /locus_tag="ACHA9N_000187"
     CDS             complement(20356..21312)
                     /locus_tag="ACHA9N_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9083686.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21483..21851
                     /locus_tag="ACHA9N_000188"
     CDS             21483..21851
                     /locus_tag="ACHA9N_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9083687.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22021..22452
                     /gene="mraZ"
                     /locus_tag="ACHA9N_000189"
     CDS             22021..22452
                     /gene="mraZ"
                     /locus_tag="ACHA9N_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9083688.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22473..23432
                     /gene="rsmH"
                     /locus_tag="ACHA9N_000190"
     CDS             22473..23432
                     /gene="rsmH"
                     /locus_tag="ACHA9N_000190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9083689.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23437..23838
                     /gene="ftsL"
                     /locus_tag="ACHA9N_000191"
     CDS             23437..23838
                     /gene="ftsL"
                     /locus_tag="ACHA9N_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9083690.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23839..26031
                     /locus_tag="ACHA9N_000192"
     CDS             23839..26031
                     /locus_tag="ACHA9N_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9083691.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26057..27019
                     /gene="mraY"
                     /locus_tag="ACHA9N_000193"
     CDS             26057..27019
                     /gene="mraY"
                     /locus_tag="ACHA9N_000193"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9083692.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27033..28394
                     /gene="murD"
                     /locus_tag="ACHA9N_000194"
     CDS             27033..28394
                     /gene="murD"
                     /locus_tag="ACHA9N_000194"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9083693.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28420..29508
                     /gene="murG"
                     /locus_tag="ACHA9N_000195"
     CDS             28420..29508
                     /gene="murG"
                     /locus_tag="ACHA9N_000195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9083694.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29523..30743
                     /locus_tag="ACHA9N_000196"
     CDS             29523..30743
                     /locus_tag="ACHA9N_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9083695.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30899..32224
                     /gene="ftsA"
                     /locus_tag="ACHA9N_000197"
     CDS             30899..32224
                     /gene="ftsA"
                     /locus_tag="ACHA9N_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9083696.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32247..33488
                     /gene="ftsZ"
                     /locus_tag="ACHA9N_000198"
     CDS             32247..33488
                     /gene="ftsZ"
                     /locus_tag="ACHA9N_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9083697.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33505..34182
                     /locus_tag="ACHA9N_000199"
     CDS             33505..34182
                     /locus_tag="ACHA9N_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9083698.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34196..34795
                     /locus_tag="ACHA9N_000200"
     CDS             34196..34795
                     /locus_tag="ACHA9N_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9083699.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34876..35097
                     /locus_tag="ACHA9N_000201"
     CDS             34876..35097
                     /locus_tag="ACHA9N_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9083700.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35146..35928
                     /locus_tag="ACHA9N_000202"
     CDS             35146..35928
                     /locus_tag="ACHA9N_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9083701.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35949..36656
                     /locus_tag="ACHA9N_000203"
     CDS             35949..36656
                     /locus_tag="ACHA9N_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9083702.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36918..39716
                     /gene="ileS"
                     /locus_tag="ACHA9N_000204"
     CDS             36918..39716
                     /gene="ileS"
                     /locus_tag="ACHA9N_000204"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9083703.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39765..41291)
                     /gene="zwf"
                     /locus_tag="ACHA9N_000205"
     CDS             complement(39765..41291)
                     /gene="zwf"
                     /locus_tag="ACHA9N_000205"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9083704.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41306..41953)
                     /locus_tag="ACHA9N_000206"
     CDS             complement(41306..41953)
                     /locus_tag="ACHA9N_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9083705.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42137..42466)
                     /locus_tag="ACHA9N_000207"
     CDS             complement(42137..42466)
                     /locus_tag="ACHA9N_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9083706.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42643..43371
                     /gene="sfsA"
                     /locus_tag="ACHA9N_000208"
     CDS             42643..43371
                     /gene="sfsA"
                     /locus_tag="ACHA9N_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9083707.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43387..44400
                     /locus_tag="ACHA9N_000209"
     CDS             43387..44400
                     /locus_tag="ACHA9N_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9083708.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44400..45677
                     /locus_tag="ACHA9N_000210"
     CDS             44400..45677
                     /locus_tag="ACHA9N_000210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9083709.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45740..48442
                     /locus_tag="ACHA9N_000211"
     CDS             45740..48442
                     /locus_tag="ACHA9N_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9083710.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48594..48911
                     /locus_tag="ACHA9N_000212"
     CDS             48594..48911
                     /locus_tag="ACHA9N_000212"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9083711.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48941..49261
                     /locus_tag="ACHA9N_000213"
     CDS             48941..49261
                     /locus_tag="ACHA9N_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9083712.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49369..50829
                     /locus_tag="ACHA9N_000214"
     CDS             49369..50829
                     /locus_tag="ACHA9N_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9083713.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50897..51118
                     /locus_tag="ACHA9N_000215"
     CDS             50897..51118
                     /locus_tag="ACHA9N_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083714.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51149..51331
                     /locus_tag="ACHA9N_000216"
     CDS             51149..51331
                     /locus_tag="ACHA9N_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9083715.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51331..51744
                     /locus_tag="ACHA9N_000217"
     CDS             51331..51744
                     /locus_tag="ACHA9N_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9083716.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51867..53048
                     /locus_tag="ACHA9N_000218"
     CDS             51867..53048
                     /locus_tag="ACHA9N_000218"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083717.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53119..53283)
                     /locus_tag="ACHA9N_000219"
     CDS             complement(53119..53283)
                     /locus_tag="ACHA9N_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083718.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53437..53510
                     /locus_tag="ACHA9N_000220"
     tRNA            53437..53510
                     /locus_tag="ACHA9N_000220"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53471..53473,aa:Arg,seq:cct)
     gene            complement(53556..53669)
                     /locus_tag="ACHA9N_000221"
                     /pseudo
     CDS             complement(53556..53669)
                     /locus_tag="ACHA9N_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(54052..55284)
                     /locus_tag="ACHA9N_000222"
     CDS             complement(54052..55284)
                     /locus_tag="ACHA9N_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9083719.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            55538..56107
                     /locus_tag="ACHA9N_000223"
     CDS             55538..56107
                     /locus_tag="ACHA9N_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9083720.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(56285..56725)
                     /locus_tag="ACHA9N_000224"
     CDS             complement(56285..56725)
                     /locus_tag="ACHA9N_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9083721.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            56883..57647
                     /gene="map"
                     /locus_tag="ACHA9N_000225"
     CDS             56883..57647
                     /gene="map"
                     /locus_tag="ACHA9N_000225"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9083722.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            57679..58602
                     /locus_tag="ACHA9N_000226"
     CDS             57679..58602
                     /locus_tag="ACHA9N_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9083723.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            58678..59817
                     /locus_tag="ACHA9N_000227"
     CDS             58678..59817
                     /locus_tag="ACHA9N_000227"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9083724.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            59810..60610
                     /locus_tag="ACHA9N_000228"
     CDS             59810..60610
                     /locus_tag="ACHA9N_000228"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9083725.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            60610..61437
                     /locus_tag="ACHA9N_000229"
     CDS             60610..61437
                     /locus_tag="ACHA9N_000229"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9083726.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            61442..62149
                     /locus_tag="ACHA9N_000230"
     CDS             61442..62149
                     /locus_tag="ACHA9N_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9083727.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            62249..63115
                     /gene="rfbA"
                     /locus_tag="ACHA9N_000231"
     CDS             62249..63115
                     /gene="rfbA"
                     /locus_tag="ACHA9N_000231"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9083728.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            63129..63701
                     /gene="rfbC"
                     /locus_tag="ACHA9N_000232"
     CDS             63129..63701
                     /gene="rfbC"
                     /locus_tag="ACHA9N_000232"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9083729.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            63723..64751
                     /gene="rfbB"
                     /locus_tag="ACHA9N_000233"
     CDS             63723..64751
                     /gene="rfbB"
                     /locus_tag="ACHA9N_000233"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9083730.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            64849..65700
                     /gene="rfbD"
                     /locus_tag="ACHA9N_000234"
     CDS             64849..65700
                     /gene="rfbD"
                     /locus_tag="ACHA9N_000234"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9083731.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            65735..67768
                     /locus_tag="ACHA9N_000235"
     CDS             65735..67768
                     /locus_tag="ACHA9N_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083732.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            67812..69092
                     /locus_tag="ACHA9N_000236"
     CDS             67812..69092
                     /locus_tag="ACHA9N_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9083733.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            69302..70108
                     /locus_tag="ACHA9N_000237"
     CDS             69302..70108
                     /locus_tag="ACHA9N_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9083734.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            70120..71340
                     /locus_tag="ACHA9N_000238"
     CDS             70120..71340
                     /locus_tag="ACHA9N_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9083735.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            71330..72916
                     /locus_tag="ACHA9N_000239"
     CDS             71330..72916
                     /locus_tag="ACHA9N_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9083736.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            72955..75093
                     /locus_tag="ACHA9N_000240"
     CDS             72955..75093
                     /locus_tag="ACHA9N_000240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9083737.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
BASE COUNT        24568 a        12816 c        16177 g        21906 t
ORIGIN      
        1 aatggtattt tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt
       61 cctcactaaa aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg
      121 ctgggatcac agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg
      181 gagctgatgc ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt
      241 tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt
      301 ctattgcggc aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat
      361 ttttacttgc agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg
      421 taattacggg gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt
      481 tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga
      541 taggatttcc gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt
      601 ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg
      661 caacagcagt tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa
      721 cattgatcag tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag
      781 tggcagttcc agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg
      841 cttcggctgg ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag
      901 gaattggaaa tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc
      961 gtacaagcgt ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag
     1021 ttgtcttact gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag
     1081 cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg
     1141 agctatttac taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta
     1201 cagttatctt tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt
     1261 ttgttgtcaa aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg
     1321 ggctgccaga aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt
     1381 tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg
     1441 tcattttctc catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg
     1501 aatttcaaga aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag
     1561 aagtagaaaa acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag
     1621 ttgctcatgc gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg
     1681 aaaaagaaca gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata
     1741 ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg
     1801 attgtagaaa atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca
     1861 ttcgtaggca tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg
     1921 atttttggta accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt
     1981 cgcaattaat tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa
     2041 tacttcggaa cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt
     2101 attgatccga tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc
     2161 ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct
     2221 aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt
     2281 aggatgaaga ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa
     2341 agaagcttct tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat
     2401 tataagaata aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa
     2461 agcagaagta ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg
     2521 gttgctgttc tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca
     2581 gaacaagtaa taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga
     2641 aggaggtgct ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg
     2701 aagtgggaca agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt
     2761 ttgacgaagt tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag
     2821 gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca
     2881 aatacaaacc taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag
     2941 tggttatcga cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa
     3001 aggaacattt aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc
     3061 tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag
     3121 cactgtcaac gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga
     3181 agaaggcggt tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat
     3241 tgctgagatt ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc
     3301 agaatatatc caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc
     3361 tatgaatcta caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga
     3421 ctcagaatca aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc
     3481 aattctttac cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg
     3541 agacgacact ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa
     3601 caaaaaacaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     3661 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     3721 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     3781 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     3841 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     3901 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     3961 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     4021 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     4081 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     4141 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     4201 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     4261 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     4321 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     4381 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     4441 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     4501 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     4561 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     4621 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     4681 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     4741 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     4801 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     4861 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     4921 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     4981 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     5041 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     5101 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     5161 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     5221 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     5281 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     5341 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     5401 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     5461 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     5521 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     5581 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     5641 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     5701 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     5761 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     5821 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     5881 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     5941 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     6001 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     6061 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     6121 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     6181 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     6241 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     6301 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     6361 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     6421 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     6481 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     6541 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     6601 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     6661 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     6721 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     6781 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     6841 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     6901 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     6961 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     7021 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     7081 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     7141 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     7201 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     7261 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     7321 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     7381 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     7441 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     7501 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     7561 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     7621 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     7681 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     7741 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     7801 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     7861 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     7921 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     7981 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     8041 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     8101 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     8161 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     8221 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     8281 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     8341 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     8401 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     8461 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     8521 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     8581 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     8641 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     8701 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     8761 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     8821 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     8881 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     8941 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     9001 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     9061 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     9121 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     9181 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     9241 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     9301 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     9361 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     9421 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     9481 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     9541 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     9601 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     9661 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     9721 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     9781 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     9841 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     9901 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     9961 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
    10021 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
    10081 agggatgttc gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt
    10141 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
    10201 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
    10261 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
    10321 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
    10381 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
    10441 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
    10501 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
    10561 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
    10621 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
    10681 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
    10741 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
    10801 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
    10861 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
    10921 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
    10981 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
    11041 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
    11101 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
    11161 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
    11221 cagtgatttt ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa
    11281 aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta
    11341 aaataaaaag aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc
    11401 tataatggaa aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa
    11461 aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca
    11521 atcttcgtta cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa
    11581 aagcattttt tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt
    11641 ttgaaattat taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag
    11701 aagaactgca ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt
    11761 tagaagaaat catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag
    11821 aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg
    11881 catatgatca catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc
    11941 aactagactt ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg
    12001 ctagcggatt gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag
    12061 gtgacttgat cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac
    12121 gtacattcgc tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag
    12181 atgctcaatt agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg
    12241 cagttgctag agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag
    12301 gacatgggat cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc
    12361 aatttgtacc aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg
    12421 gagtacgtat tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt
    12481 caccaaaaga attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag
    12541 tgttcacaga ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt
    12601 taagctgtaa gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga
    12661 tcggcggcag ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat
    12721 ctcgtgctta ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa
    12781 cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg
    12841 gattgcagct tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt
    12901 cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga
    12961 tggcttgaaa gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc
    13021 aatggaaatg caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc
    13081 tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga
    13141 agaacttttt gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc
    13201 gggaaatggc attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag
    13261 atgcgatttt caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag
    13321 agtttgttcc tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat
    13381 tggataaagt gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa
    13441 tggatttggt gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag
    13501 cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac
    13561 gtaaattcgt caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag
    13621 tcctagcttt tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta
    13681 gaatgaaaac acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag
    13741 aattagctga aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg
    13801 gtatccatcc cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg
    13861 taagaaataa agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc
    13921 ctgagacgat ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta
    13981 gttttcatgg tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc
    14041 tgttagctat ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac
    14101 tccttgcggg taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg
    14161 aagcttattc tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg
    14221 tcggtaaacc gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt
    14281 ctaaaaccga aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag
    14341 gacgtggaca ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag
    14401 ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc
    14461 ctattgcagg ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc
    14521 tgatgtatca aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag
    14581 aatatttaac tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt
    14641 atttagaacg cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc
    14701 atcaatattt cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag
    14761 cattccaaaa attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta
    14821 tttcattata tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg
    14881 gaataggtgc tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc
    14941 tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca
    15001 gtccaagcgg agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg
    15061 tccaattggt tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca
    15121 ggaatatcca gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg
    15181 gtggttcgat tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc
    15241 aagctaatac gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt
    15301 tagtagcaga tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt
    15361 tgtcagaaga gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac
    15421 gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc
    15481 agcctgaaag attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat
    15541 atcaagctac acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc
    15601 aattggaaca agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc
    15661 tagaaaaacg cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg
    15721 ctcaagctgt tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca
    15781 gctatacgac gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt
    15841 tgctgacgat ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag
    15901 gagaataaaa atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt
    15961 gtcatgcagt tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga
    16021 ggaatggaat taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg
    16081 aaaatgatga cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat
    16141 gagattaact gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt
    16201 tttagatgag catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc
    16261 aggcgggaaa agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc
    16321 tattgatgtt cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt
    16381 gatccacgat gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa
    16441 tcataaagta ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc
    16501 attccagcta atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca
    16561 attagctgtt tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg
    16621 cgaatcgaaa aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca
    16681 tttgattcgt tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt
    16741 attgctactt caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct
    16801 actcgatgtg attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat
    16861 ggaaaaaaat acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat
    16921 tgagctgtta tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga
    16981 tggcgggctg atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg
    17041 gaaaaagaac gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa
    17101 gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa
    17161 ccgggagaaa aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat
    17221 gtttctcgag ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa
    17281 ggaaaaacat tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa
    17341 aatggcgcaa aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata
    17401 agacaagatc ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa
    17461 gactttacct taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa
    17521 ctgatgctgc ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc
    17581 aaaccccaat tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct
    17641 cagacccatc aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat
    17701 gtgctggaat taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct
    17761 cacttgagga aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg
    17821 gtttcaaatg cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga
    17881 gaagtatgcg gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa
    17941 tatgctaagg agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt
    18001 gttgagtgag caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg
    18061 aatatctgtt actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt
    18121 accggcttca gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa
    18181 tactaaattg gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt
    18241 tgttattttg aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg
    18301 ttacaagaaa gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg
    18361 aactgaagac caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga
    18421 gcgaatgtga tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg
    18481 ctttcttttc acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt
    18541 attatcgatg ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa
    18601 ggagcagaaa aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct
    18661 gaactgatgg ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat
    18721 atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat
    18781 ctgagaagaa tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg
    18841 ctccagcctg aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag
    18901 aagaaaaaga gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga
    18961 aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa
    19021 gaagaaatag ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga
    19081 gaaaaactta gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg
    19141 acgaataatg aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg
    19201 atcgcccatc tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat
    19261 ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag
    19321 agtcgtttag aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac
    19381 ggcgaatcca ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct
    19441 tctgattttt cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt
    19501 ctacttcagc aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg
    19561 gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta
    19621 cgtttcttaa aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt
    19681 tacattacga ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc
    19741 gaactgtctc ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca
    19801 agtattattt ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca
    19861 gataaaattt ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa
    19921 gttgctgcag tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg
    19981 caaacttctg tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg
    20041 gctggaagtg agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca
    20101 aaaaggacct gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt
    20161 ttatttttac atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag
    20221 cattgtccaa atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg
    20281 cattgttttc aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc
    20341 cacaagaggg cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca
    20401 aataaataat gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa
    20461 cttaatgttc attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa
    20521 agaagttaaa attttcataa tattattcag attattcgat acgattgctg aaaaagtctg
    20581 attcatttgt tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc
    20641 caccaataca tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg
    20701 cgctagttct ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct
    20761 tttttggata tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc
    20821 ttccaaagta tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac
    20881 ctgccaaatg aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt
    20941 ctggctaaag actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt
    21001 tttgggcgtc aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact
    21061 tgttttagca aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt
    21121 gaattcttct gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg
    21181 aatttcaaat tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata
    21241 ccagatcgtt tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa
    21301 atatttgatc atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt
    21361 ggaatacatg gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg
    21421 aaacagttgt gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt
    21481 tcatgggcac attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt
    21541 ttggttgcca atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc
    21601 ctgatttggc gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga
    21661 caatttctat cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg
    21721 ttttccaagc atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc
    21781 gtttggtttt tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt
    21841 cttacctatg aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt
    21901 gatttttaaa aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg
    21961 gtagaaagtg ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca
    22021 atgttgatgg gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca
    22081 aaacttcgtg aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt
    22141 ttcggctatc cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg
    22201 gctaaaaaag acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa
    22261 cttgataaac aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca
    22321 aaaggttgcg tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg
    22381 caagattttt ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat
    22441 ttcggactat aagaatagga agaggctgac taatgactga aacatttcaa cactatacag
    22501 tcatgttgaa agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact
    22561 gtacactcgg cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc
    22621 atctgtatgc gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt
    22681 cttatgtcga acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac
    22741 gcttgagtga gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc
    22801 ctcagctaga tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc
    22861 gcatggatca gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg
    22921 aattagtaaa gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg
    22981 aaatcgaacg agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca
    23041 ttaaacaagt catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt
    23101 ttcaagctgt gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac
    23161 aggcaatccg gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg
    23221 aagacagaat tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg
    23281 gacttcctgt catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa
    23341 tccttccttc agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta
    23401 ttgcagaaaa aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa
    23461 gattacgaat atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct
    23521 tcttcgaatc aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct
    23581 gctttggaaa aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta
    23641 acagttatgc tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca
    23701 gaaatcaatg ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct
    23761 cgtactgaac ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat
    23821 ttaaggaaag tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa
    23881 aatctaaatc cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt
    23941 ggattgttct ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct
    24001 ggtacctcat tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca
    24061 aaaagaggaa cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat
    24121 tcaattaaag cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa
    24181 gaaaagaatt ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa
    24241 gatgcactga atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt
    24301 agctatggga aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa
    24361 gaaggtgtcg ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc
    24421 ttttcttctc attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt
    24481 aaactaggtt tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt
    24541 tatcaaaaag ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct
    24601 gtcgacggac aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca
    24661 ctgatggatc aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa
    24721 gcaaaaaccg gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg
    24781 gaaggattga ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa
    24841 ccaggatcaa ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac
    24901 gagaatgaaa ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat
    24961 gactttggtg aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc
    25021 gggatggtca tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg
    25081 ggatttggac aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca
    25141 ttaaatattg tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc
    25201 caaatgatga aagctttcac ttctattgcg aataacggta caatgattca acctcgatat
    25261 atcagcaaag tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta
    25321 gggcaacctt tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc
    25381 gaaagcgaaa actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg
    25441 gcaaaaaacg gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca
    25501 gcctacctgt attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat
    25561 ctaacaatga aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca
    25621 ttgatgaaac gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa
    25681 acagaaaaag tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat
    25741 gctcaaaaaa gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa
    25801 tctactgcca acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt
    25861 gataagctga tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa
    25921 atcctcggta ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct
    25981 ccatatgaaa aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa
    26041 taggagagaa aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc
    26101 aatgacaatt gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca
    26161 ggcgattcgt gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg
    26221 cggcttagtg tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa
    26281 acagctaact cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg
    26341 ctttcttgat gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca
    26401 gaaattgatt ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg
    26461 ttatccaggt gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat
    26521 tttcgctctc ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga
    26581 tggattagtt gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca
    26641 tcagcatcag tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt
    26701 cttcgtctac aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt
    26761 gggcggactt ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat
    26821 tggtttgatc tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct
    26881 tacaggaaaa cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg
    26941 gtcggagtgg aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac
    27001 attatggatc gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata
    27061 agaaagtgct tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg
    27121 aattgggcgc acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac
    27181 aagagctgct ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg
    27241 atgaagaatt ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag
    27301 caaaagccca agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag
    27361 cggaatgccc aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga
    27421 caggactact actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg
    27481 ggtatccagc aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat
    27541 tgtctagctt ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca
    27601 atatctatga agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat
    27661 ggaaccttca aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag
    27721 aactacaaga attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag
    27781 tgttggaaga cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa
    27841 tggaaatatc tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta
    27901 tttctgtagc aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt
    27961 tccatggcgt accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata
    28021 atgattcaaa agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt
    28081 ctaaggtcgt gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc
    28141 cttcattaaa aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg
    28201 atgctggaaa aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag
    28261 ttcctttagc attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg
    28321 ccagctggga tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga
    28381 accgattaaa atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc
    28441 ggaggaacag ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa
    28501 gaaccgaatg ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc
    28561 cctgatacag gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc
    28621 atgcataaca tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc
    28681 ctcagtgatt ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta
    28741 gtgtacgctg cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca
    28801 ggtgttacca ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat
    28861 gcggcttcct tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa
    28921 gtagcagata ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa
    28981 acagtcttga tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt
    29041 tttttaagtg aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat
    29101 tatgaagaga tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat
    29161 atcaaaaata tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca
    29221 acttcgatcg cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat
    29281 gttacgaatg atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa
    29341 atgattgcag ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg
    29401 ggtgatgaag aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat
    29461 gcttcaaaac gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct
    29521 tagtgagtaa gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt
    29581 tgactccttg gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag
    29641 agccaaaaaa agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa
    29701 cggttcaaga ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag
    29761 agatcaagca aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca
    29821 atttaaaaag tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat
    29881 tgggtatacc gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag
    29941 ttgcagtatc aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt
    30001 cattaggaga aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta
    30061 aaaaagcaca gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg
    30121 atttggatgt aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc
    30181 cagtgattga aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc
    30241 ctatcttgga agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc
    30301 agttatcatc agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca
    30361 ataaaaatct gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata
    30421 atcttgcgaa ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg
    30481 tgatcgacat ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag
    30541 aatcgggcca atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat
    30601 caagtgaaga ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat
    30661 catctgattc aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc
    30721 cgtcaagttt aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa
    30781 aaccagtaat ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta
    30841 aaacggacgc tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat
    30901 ggcaaaaaca ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt
    30961 ggctgaatat atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg
    31021 gattaaccga ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt
    31081 acgacaagca gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc
    31141 aaacatgctt gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga
    31201 aatcaccgat gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc
    31261 tgaacgtcaa atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat
    31321 caaagatcct cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg
    31381 acctaaaacg atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa
    31441 cgaaatggtg attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga
    31501 ttttggtacg atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga
    31561 taagcaattg aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat
    31621 ctctattgta ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga
    31681 tgcataccca gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc
    31741 agaaccagtg aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca
    31801 gattttcaac aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg
    31861 cgtcgtattg acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat
    31921 cttcggcgta aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt
    31981 tacaaatgta ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc
    32041 aaaaattgct gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac
    32101 aacaaacaca tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc
    32161 aaaagagtcc ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga
    32221 ttaattcatc ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg
    32281 gcgcagtaat caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga
    32341 ttgaagaaaa cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa
    32401 aaaattcaaa agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg
    32461 ctggttctca gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg
    32521 aagctttaga cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta
    32581 caggtgctgc accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg
    32641 tcgtgacacg tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta
    32701 tcgctcgttt gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag
    32761 aagttgtaga taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac
    32821 gacaaggtgt acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact
    32881 ttgctgacgt gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg
    32941 ctagtggaga agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg
    33001 aaacatctat tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga
    33061 cattgttcga agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa
    33121 atatcattct aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca
    33181 tcgcaacagg tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag
    33241 cacaaatgca atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt
    33301 cttcagaaga agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc
    33361 ctagagttga tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc
    33421 gcgaagaaac aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta
    33481 aaaaataaag gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa
    33541 attcaggatt cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta
    33601 acaaaatctg ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt
    33661 gcagaaaacc gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa
    33721 ataaagtggc atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa
    33781 attgattact ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta
    33841 gagaaagaat tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga
    33901 atttcaccag atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag
    33961 gttgttgggt tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg
    34021 tttggtacgc tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca
    34081 tgtagtgaat taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca
    34141 acatttatcc gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc
    34201 gcttttaaac agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga
    34261 ataccaagat tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac
    34321 acagaatcaa tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc
    34381 gacttcacgt cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga
    34441 actacatcaa tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac
    34501 acaacctaaa gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga
    34561 agctatggcg attgctaaac gaatcattgc tggagaagca accttagtga attttcattt
    34621 agtagaagaa aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact
    34681 agatggagat atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga
    34741 gattgacagt tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga
    34801 ggaactacga tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc
    34861 tatagcatta tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca
    34921 gggctaggac gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg
    34981 cctttgagga ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta
    35041 gccgtacaag gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg
    35101 aagcaacaaa atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta
    35161 taccagcact ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa
    35221 caggttcaag gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat
    35281 attttagaaa cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat
    35341 gaacaggcag aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa
    35401 gattttgaaa cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat
    35461 gggaaaatcc ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc
    35521 atctcggacg gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct
    35581 gcacaagtaa caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa
    35641 atgattatgc cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta
    35701 gacagtgtga tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa
    35761 gctggtaaag taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta
    35821 ttagatatta tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag
    35881 acgaaaaaag aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg
    35941 gtttactcat ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc
    36001 ggggatataa tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg
    36061 aattgactca aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg
    36121 agtacttcaa tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg
    36181 ctgataaagt aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa
    36241 acaaagcaga tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg
    36301 cagaagaaaa agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga
    36361 cagaaactga agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc
    36421 ttgagtctca attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct
    36481 ccagctacgt gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca
    36541 atgaaaatga tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat
    36601 ttgatacaca ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca
    36661 gaaaagcact ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg
    36721 catggtgaaa gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg
    36781 ctgacctcgt tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg
    36841 taccacggca tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata
    36901 aaggagtgta tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc
    36961 ctatgcgtgg taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc
    37021 gaatctacca aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg
    37081 gacctccata tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag
    37141 acattatcat tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg
    37201 atacacatgg attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa
    37261 tgtccatggc agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc
    37321 aacgagcaga tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat
    37381 tagatccttc ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct
    37441 atatttacaa aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg
    37501 aagctgaaat tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca
    37561 aagacggcaa agatattctt gatgaagata cagcttttgt catttggacg acaacaccat
    37621 ggacattgcc agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg
    37681 aagccgatgg ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa
    37741 tcggttggga aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga
    37801 ctgcaagtca tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt
    37861 tagacgctgg tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg
    37921 ctggtagcaa atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag
    37981 aagaagcacc tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc
    38041 tattaaaaga aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg
    38101 attggcggac gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg
    38161 ataaattccg tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg
    38221 gtaaaacacg tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc
    38281 gtgcatgggg agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac
    38341 cagaaacaat cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg
    38401 aaaaagaagc gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg
    38461 aatttacaaa agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag
    38521 ctgttttacg tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag
    38581 accaatatcg tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag
    38641 caccatacaa atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga
    38701 gtaaatcttt aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata
    38761 tccttcgctt atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata
    38821 tcttaagtca agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag
    38881 cgaatacaga agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg
    38941 tagacaaata tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt
    39001 acgacaaata taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc
    39061 tatcgtcatt ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt
    39121 atcaacgtcg ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat
    39181 tgactcctat cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg
    39241 agtacgttca gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga
    39301 tggatacctg gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag
    39361 caagaaacag caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac
    39421 aacaagttcg tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt
    39481 caccagacta ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat
    39541 ttgatgatgt tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc
    39601 aagttcgtaa agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa
    39661 aaatcgttga agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg
    39721 gaaaaggggg tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt
    39781 ccacgttcac gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt
    39841 agttcgaaag ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt
    39901 gtttggtccc aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc
    39961 caatgagaga agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct
    40021 tctgggctat tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt
    40081 tcaaatcctt gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga
    40141 atatagatcg ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat
    40201 gttttgaata cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca
    40261 gtcaaacgtt tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata
    40321 gtgaatttgc ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg
    40381 cgatagccct tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag
    40441 ttttctaaca cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct
    40501 gtacggattt ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc
    40561 tgtaaaatat gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg
    40621 cgatcttcta caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg
    40681 ttccattgag cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt
    40741 tcttttccta aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt
    40801 tgctggttca attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa
    40861 cgattgaagc cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca
    40921 aaaaattgag gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca
    40981 tctaatcggt cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg
    41041 gattgatagt aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca
    41101 cggattgttt ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt
    41161 ccgattacag caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg
    41221 tacaatttac gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt
    41281 tcattagaca tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct
    41341 taaagctgac ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg
    41401 cgatgtcaac gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca
    41461 acaattcttt ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact
    41521 ccaatgtttg tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat
    41581 gaggacaatg ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag
    41641 tcacatgttc caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt
    41701 caactaaaaa aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc
    41761 cgcttgcagt caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa
    41821 gtttagaaat catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt
    41881 tattgttgac tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc
    41941 gatttggtgt catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac
    42001 aaaaaaaacg ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt
    42061 acaacaaaaa acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat
    42121 atacattggg agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt
    42181 cataaagagt ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga
    42241 ctaaacgata ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa
    42301 acgtgtaatc agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg
    42361 taccttgata ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa
    42421 taaaacttga gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca
    42481 aatcactcct ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt
    42541 accgaataaa agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac
    42601 aaattatgat aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg
    42661 gaaatagctt tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac
    42721 ggagaaataa ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca
    42781 ggcgctgaag tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg
    42841 attgctgtaa aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg
    42901 gcaattgatg gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg
    42961 gtatacaagc gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg
    43021 gtcggacaaa aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt
    43081 gcttttcctg atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc
    43141 gcacaagatg gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg
    43201 gcaacgatcc atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa
    43261 agcggagttc aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag
    43321 caggccattc catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt
    43381 aaaataatga tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa
    43441 gcagctttaa gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa
    43501 gcacaggaat ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact
    43561 tttttcaata ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt
    43621 agtcaagcta agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt
    43681 tcgacgccaa aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt
    43741 tttgaagcag caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca
    43801 ttaaaagacg agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac
    43861 caagtattag atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg
    43921 atggatttag gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa
    43981 gtccattatt tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta
    44041 cgttatgaga attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg
    44101 tcagagattt attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa
    44161 gcacaatttc atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa
    44221 aatccaatgg cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca
    44281 gcttggggat tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt
    44341 tatgatttaa gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa
    44401 tgaacgaatt tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt
    44461 ttatctcacc ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta
    44521 tagtagcggt ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac
    44581 taaacgtgga accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag
    44641 ctattcaagt agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat
    44701 ctttggtggg cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa
    44761 tcttgattgg tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac
    44821 atcaagtgaa aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc
    44881 aaatcattga tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag
    44941 cgacagcaga tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa
    45001 ttgacgtgac agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac
    45061 cgatacgaaa gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc
    45121 tgattttctt taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag
    45181 gtgtaccggt aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag
    45241 aacaattcaa aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt
    45301 tagatattac aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt
    45361 atttgcatcg ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg
    45421 tacaagataa taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg
    45481 aagagatttt tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag
    45541 ctcctgcagt aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat
    45601 ataacaaaga aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag
    45661 gagcaaggcg caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg
    45721 ttgccagagg aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga
    45781 aaaccaacaa ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa
    45841 tgcaagccga tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg
    45901 accagtcgtt tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc
    45961 ttctacgcaa gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat
    46021 acagcaggca aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg
    46081 agaaactggt acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc
    46141 agagatgctt tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa
    46201 tccgcaactg ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc
    46261 tcaagatagt cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat
    46321 ccaccgttta ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta
    46381 ccgtccgctt ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac
    46441 gactgaatct tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac
    46501 cttgccgaat cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat
    46561 caaacaagaa gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc
    46621 cttcatgctt tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt
    46681 ctgtgcaaaa tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa
    46741 agaagaactt tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg
    46801 tctcgaccga ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga
    46861 tgttgtctgg tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa
    46921 agtcacttct ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa
    46981 agatgtggat gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa
    47041 agatcttttg ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa
    47101 acggttagac cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag
    47161 tacattggat cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg
    47221 gaaaaatttt aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga
    47281 agaacaagtt gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa
    47341 tctaggagaa agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg
    47401 cagacagact gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg
    47461 gaaagctttt aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca
    47521 atatgtcaaa aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg
    47581 atcattaaac accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat
    47641 cacgatgaca agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag
    47701 cttagaagat atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt
    47761 caaagcggaa aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac
    47821 tggtgaagga gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa
    47881 ggtagaactg atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc
    47941 cttattggaa gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa
    48001 tatcccattt ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg
    48061 tatagctgat gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag
    48121 cgatgtcttt tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca
    48181 gaccgatttg tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc
    48241 cttcatgtta gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat
    48301 tttctcaaaa atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga
    48361 acgaaaatat caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct
    48421 tgaaaataaa ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg
    48481 tgcgcactat aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt
    48541 ggcatgcttc ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa
    48601 aaaaaacaat catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa
    48661 tgcaaaaagc agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag
    48721 atgcagataa taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac
    48781 gtttcatgaa agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca
    48841 tcaatatgca agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc
    48901 taatgaaata aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct
    48961 agaagccatc atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc
    49021 gattgcggct gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga
    49081 agaatcactt atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg
    49141 aaatcatatg caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat
    49201 tgcgtttact gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata
    49261 aatacaactt cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt
    49321 ataaggagaa ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta
    49381 tcaagttgga tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat
    49441 ttagttgctt tgcgtgatgc ttttatcggc acactgcccg caacaatggc gggatcagtc
    49501 gctgtaatga tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct
    49561 gacagtttag cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat
    49621 ggttttgtct ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg
    49681 tataatcttg cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct
    49741 accttgattc aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt
    49801 ccttcaaatg tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc
    49861 acactgtcag ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat
    49921 tttacagtta tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc
    49981 aacattacga tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc
    50041 attttaccag cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt
    50101 gtagctgatg ggcaactgat cattgatttg gtgcaaaaat atattgcaga gccattccta
    50161 ggattatctc aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg
    50221 ttctttggta ttcatggacc aaatgtattg gcaccagtgc tagagggaat ctggggacaa
    50281 gcacaactaa tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca
    50341 gccgtgttag atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat
    50401 gcttttgcat ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt
    50461 ttctctaaac gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc
    50521 aatatcaacg aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc
    50581 ccattcttga ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc
    50641 ctagtcgctc cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc
    50701 ttagcaacag gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact
    50761 ttcttgattt gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa
    50821 caacaataag atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt
    50881 tcgaggagga actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga
    50941 attgttagaa ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat
    51001 caagctgttc tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga
    51061 actaatcaaa gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa
    51121 aatggtttaa aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat
    51181 tggttctttg atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag
    51241 tatggtcaca ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa
    51301 aagagaagaa gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca
    51361 gcatcgtttt tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc
    51421 ggcaaatcag taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa
    51481 aataaccggg ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt
    51541 acatcgacaa cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg
    51601 acctgtgagg ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat
    51661 gatatgcttt taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta
    51721 gatatgatcg aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa
    51781 tgaagtaagc tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta
    51841 gcttttttgt aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt
    51901 cgattgagga aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa
    51961 ggcgagaacg ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg
    52021 aggatcggct aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag
    52081 agtacctaat ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg
    52141 ggatcaatca gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat
    52201 cttatctggc gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg
    52261 ataaaatcag ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa
    52321 aaataaagcg tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga
    52381 tgccggaaaa ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca
    52441 agcaaaaagg caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat
    52501 atatcgttta tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc
    52561 atgatgcttt ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat
    52621 ttgaatattc atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg
    52681 cttctgtaat gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg
    52741 aaaattatcc atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt
    52801 atgcgaaatg gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct
    52861 caaaagtaga acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc
    52921 tattattagg gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa
    52981 aagaatatga agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact
    53041 atttttgata aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt
    53101 ggcatttgaa ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt
    53161 ttcttttgtt ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc
    53221 ccgatttttt ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt
    53281 cattggaaag gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga
    53341 ctagtggaaa cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt
    53401 aaaaaacaaa caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga
    53461 gcaatcgcct cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tacaaataac
    53521 ccctgtatcc tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa
    53581 ctgctatgtc ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata
    53641 gatgcatgcg tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg
    53701 attactaaag aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt
    53761 cgaattaaac gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct
    53821 taactctagc ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga
    53881 ctttttatta tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt
    53941 accatataaa aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag
    54001 atcctttatt ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca
    54061 atcgccattt cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat
    54121 cttgtcagat cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc
    54181 taattcttca tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag
    54241 tgctaagtga ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg
    54301 agaaataggt gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc
    54361 tgtatcaagc agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt
    54421 tccatcttta aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt
    54481 tgtacttgaa atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt
    54541 cggcatattt gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag
    54601 atggttttta ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga
    54661 aaattgttct cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca
    54721 tgctaagaca gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc
    54781 ctcaacttgt ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc
    54841 cgcagccacg acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag
    54901 tgctttgcca ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc
    54961 atcgggatcg gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc
    55021 ttgatcgatt ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa
    55081 ttctctttgg atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata
    55141 agcttcttgt gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac
    55201 tgtgtgtcct ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag
    55261 aagttcatta aaatcagata acataataaa aattcctcct caccattttt tctggtaact
    55321 attagcatga taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg
    55381 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
    55441 gaaaatcaaa aactaatttt gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg
    55501 tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac
    55561 tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt
    55621 tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt
    55681 taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata
    55741 tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat
    55801 ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg
    55861 aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc
    55921 agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga catggatcat
    55981 agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat
    56041 tgaaaagatg atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat
    56101 cgattgaata tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa
    56161 ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga
    56221 atgcaatgaa taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag
    56281 tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt
    56341 cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa
    56401 aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc
    56461 cgcagacacc gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta
    56521 cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg
    56581 cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca
    56641 gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag
    56701 catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta
    56761 tgaattatac caaaacatca ttgaaaagaa aataaaacct aaagacacct ggggaatgtc
    56821 atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa
    56881 atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat
    56941 tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata
    57001 ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct
    57061 ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc
    57121 ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga
    57181 aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg
    57241 accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag
    57301 ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag
    57361 cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag
    57421 ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga
    57481 tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg
    57541 cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag
    57601 atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa
    57661 taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg
    57721 cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc
    57781 gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt
    57841 ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct
    57901 gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga
    57961 ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct
    58021 ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc
    58081 cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc
    58141 gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg
    58201 aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc
    58261 atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt
    58321 ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat
    58381 gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc
    58441 atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt
    58501 gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg
    58561 ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg
    58621 taaatttata tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg
    58681 cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta
    58741 acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga
    58801 agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt
    58861 atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg
    58921 attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct
    58981 aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt agcagatata
    59041 cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt
    59101 cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg
    59161 gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat
    59221 tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt
    59281 attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga
    59341 gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact
    59401 tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt
    59461 gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt
    59521 catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg
    59581 atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg
    59641 atcgtattga ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg
    59701 atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac
    59761 cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa
    59821 attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac
    59881 actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga
    59941 caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat
    60001 cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat
    60061 agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac
    60121 agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa
    60181 gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga
    60241 ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc
    60301 tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg
    60361 tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt
    60421 cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt
    60481 attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc
    60541 aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg
    60601 ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt
    60661 caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt
    60721 atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt
    60781 tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa
    60841 gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat
    60901 tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg
    60961 attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca
    61021 gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt
    61081 gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta
    61141 gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc
    61201 cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat
    61261 cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat
    61321 ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa
    61381 aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt
    61441 cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga
    61501 tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt
    61561 agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt
    61621 ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg
    61681 agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat
    61741 cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt
    61801 ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg
    61861 atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc
    61921 tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg
    61981 tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca
    62041 tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg
    62101 tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt
    62161 taaatcctta atatttccat gacaaatata acaattgatg agataataac aaagtgaaat
    62221 ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg
    62281 aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa
    62341 accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat
    62401 tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt
    62461 aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat
    62521 cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta
    62581 ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac
    62641 agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa
    62701 tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac
    62761 tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga
    62821 acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc
    62881 cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct
    62941 tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt
    63001 ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca
    63061 gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt
    63121 ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg
    63181 tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac
    63241 atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc
    63301 ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca
    63361 ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat
    63421 gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg
    63481 cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact
    63541 ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc
    63601 caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag
    63661 aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc
    63721 ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt
    63781 acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag
    63841 gaaacaaaga gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg
    63901 ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg
    63961 cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata
    64021 tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg
    64081 tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg
    64141 aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt
    64201 ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg
    64261 caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc
    64321 ctcgccaagt aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga
    64381 atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga
    64441 aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg
    64501 tgatggaatt gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg
    64561 accgcgcagg gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag
    64621 gttggcagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg
    64681 aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa
    64741 atggacaata atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc
    64801 gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta
    64861 actggaggaa atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg
    64921 ccatatgttt caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct
    64981 tatattacag aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat
    65041 aaagcagaag aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat
    65101 gtagcacttg cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt
    65161 ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat
    65221 gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat
    65281 tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg
    65341 caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca
    65401 acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt
    65461 ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata
    65521 ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca
    65581 acacgtccgc agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata
    65641 ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa
    65701 tttccttttt atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg
    65761 acgaaaagaa aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct
    65821 aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa
    65881 cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac
    65941 tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc
    66001 tttttacctc tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa
    66061 cggacaaaac atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa
    66121 accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc
    66181 gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag
    66241 gaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg
    66301 gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt
    66361 ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt
    66421 ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc
    66481 gtacttgctg attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg
    66541 ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt
    66601 tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg
    66661 aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa
    66721 ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta
    66781 tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag
    66841 cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat
    66901 taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt
    66961 aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta
    67021 tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc
    67081 atggtcttat ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga
    67141 aaccatcgca gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag
    67201 acgcttgttt tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc
    67261 agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat
    67321 cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc
    67381 aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa
    67441 gaaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc
    67501 ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac
    67561 gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt
    67621 aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga
    67681 cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac
    67741 tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag
    67801 tagaaaggag agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg
    67861 tcggtttctt gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt
    67921 ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta
    67981 ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg
    68041 cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg
    68101 gagacttact gtttcctttg gttattttat tttgtttagt aattaaatgg atcgagtgga
    68161 caaaatggga tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat
    68221 atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt
    68281 cgctttctga tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt
    68341 tattgatcag tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga
    68401 tagcagtcgt tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg
    68461 cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg
    68521 cgccatttat tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg
    68581 ctgtctctga aaaagtcaat ttgtttaccg atcgagaaga tatttggcat aaattctatc
    68641 agacgctagg agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc
    68701 gaggaaacca atttttagga aaccacaata gttataattc tattttgaat atctatggat
    68761 tgataggatt tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga
    68821 aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc
    68881 agtcatttat ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt
    68941 tggctatggc cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg
    69001 aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg
    69061 ctcattcttc caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga
    69121 caaccgtcgc agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa
    69181 cgttagagaa aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac
    69241 caaaaatatg gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca
    69301 aatgtttaaa gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct
    69361 acagttttcg atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg
    69421 ggcgtttatc aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact
    69481 gaaagcacaa atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat
    69541 tccctggttc ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac
    69601 ttatttagta aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc
    69661 caatctgtac acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg
    69721 tatatatcct tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt
    69781 cttgacagga ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca
    69841 attaattacg atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa
    69901 tcagtttgat tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca
    69961 aggttatcgt gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt
    70021 gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt
    70081 gaaaccacat ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga
    70141 ctaaaagata tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa
    70201 gccctcaatc cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat
    70261 gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct
    70321 acaatgttga aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa
    70381 ggaaaaatct cagctttgct agaactagga tcaggattca atcctgagta ttctggctat
    70441 gaaaatatct atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg
    70501 gtcgatgata tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact
    70561 tattcaagcg ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat
    70621 attttgattg tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg
    70681 gaaaaattta cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc
    70741 agttcgattc gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa
    70801 tatggcgata cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt
    70861 aaaacagtgg atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac
    70921 gtgacgaaag cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga
    70981 gaaaaactat acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt
    71041 ttaggaattg cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta
    71101 gatggtatca agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag
    71161 ttaggtttgt tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg
    71221 gttcctttgg tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga
    71281 gaaggcatcg tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga
    71341 ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc
    71401 aggaagtaaa gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg
    71461 agcaaaacaa tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag
    71521 cgaatttgct caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta
    71581 tgctggacag acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc
    71641 tggaaaaaca ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac
    71701 attccctaac ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg
    71761 ggcttcttat ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca
    71821 aaaagttttt gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt
    71881 aggtgataca gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt
    71941 caaaatgcga ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt
    72001 agcacaaagt tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac
    72061 ccagcaaacg aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga
    72121 aaatgagaca ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc
    72181 gcaacaacct agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat
    72241 cgattcgaat gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt
    72301 accagagttc gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg
    72361 ctttatcggg gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc
    72421 tgaagtcaca aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct
    72481 tcttgaggaa aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac
    72541 attgttggaa agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac
    72601 ttcgaaaaaa atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc
    72661 agaaacaaaa atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt
    72721 gtcctataag ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga
    72781 agaagtcaat caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat
    72841 cctttgcgag caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg
    72901 gatcattgaa atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa
    72961 aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg
    73021 cttattgtgg gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa
    73081 aaagaaaatg tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat
    73141 aatcttgatg ttaagaccca aagcggtttc aaggtacgtt tatcagggaa aatgagagga
    73201 aaagccatct tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac
    73261 ggtaaatacc cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg
    73321 aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa
    73381 ttggaattga agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca
    73441 gatttgaaag agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata
    73501 cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg
    73561 gatcaaacgt atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct
    73621 atccgaaagt gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga
    73681 aaagaaaatg gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa
    73741 tttatagcac tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca
    73801 aaagtgctaa acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat
    73861 gcagatggca atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg
    73921 ggaaataatt atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc
    73981 ggtttccgta aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa
    74041 caaatccctg cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc
    74101 ccaggatcaa ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa
    74161 gcattgacag atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt
    74221 tctggatttt atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt
    74281 ccaacgaaga atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg
    74341 tcttattcaa attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa
    74401 gaattatttg cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac
    74461 attccattca attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac
    74521 tttttattct tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt
    74581 tcttatgcac agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat
    74641 acaacgcagc atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat
    74701 aactatgatc gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta
    74761 gcagtcactg cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt
    74821 gatgaaacat tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg
    74881 ggtcgttaca atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga
    74941 ggttatgaag atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa
    75001 gataaatggc cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagac
    75061 ggaatcggcg acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg
    75121 attcgctcgc acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga
    75181 ctcaaaaaaa taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat
    75241 tcacgaaaat tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc
    75301 tgctcccacc gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc
    75361 acgctcttat tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa
    75421 ccctagctgt caaagaatcg atattttatt ttaatatagg ctatttt
//
LOCUS       DAYYJC010000004        68317 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000004 DAYYJC010000000
VERSION     DAYYJC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.004"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..899
                     /locus_tag="ACHA9N_000241"
     CDS             18..899
                     /locus_tag="ACHA9N_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9083738.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ACHA9N_000242"
     CDS             912..2522
                     /locus_tag="ACHA9N_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9083739.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ACHA9N_000243"
     CDS             complement(2599..3258)
                     /locus_tag="ACHA9N_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9083740.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ACHA9N_000244"
     CDS             complement(3541..4206)
                     /locus_tag="ACHA9N_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9083741.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACHA9N_000245"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACHA9N_000245"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9083742.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACHA9N_000246"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACHA9N_000246"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9083743.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACHA9N_000247"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACHA9N_000247"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9083744.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ACHA9N_000248"
     CDS             6520..6840
                     /locus_tag="ACHA9N_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9083745.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..7809)
                     /locus_tag="ACHA9N_000249"
     CDS             complement(6952..7809)
                     /locus_tag="ACHA9N_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9083746.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7917..9220)
                     /locus_tag="ACHA9N_000250"
                     /pseudo
     CDS             complement(7917..9220)
                     /locus_tag="ACHA9N_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9217..9726)
                     /locus_tag="ACHA9N_000251"
     CDS             complement(9217..9726)
                     /locus_tag="ACHA9N_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083747.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9901..12036)
                     /locus_tag="ACHA9N_000252"
     CDS             complement(9901..12036)
                     /locus_tag="ACHA9N_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9083748.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12119..13774)
                     /locus_tag="ACHA9N_000253"
     CDS             complement(12119..13774)
                     /locus_tag="ACHA9N_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9083749.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13776..14090)
                     /locus_tag="ACHA9N_000254"
     CDS             complement(13776..14090)
                     /locus_tag="ACHA9N_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9083750.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14260..14520
                     /locus_tag="ACHA9N_000255"
     CDS             14260..14520
                     /locus_tag="ACHA9N_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9083751.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14534..14848
                     /locus_tag="ACHA9N_000256"
     CDS             14534..14848
                     /locus_tag="ACHA9N_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9083752.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15578..16132)
                     /locus_tag="ACHA9N_000257"
     CDS             complement(15578..16132)
                     /locus_tag="ACHA9N_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9083753.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16258..18186)
                     /locus_tag="ACHA9N_000258"
     CDS             complement(16258..18186)
                     /locus_tag="ACHA9N_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9083754.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18492..19208)
                     /locus_tag="ACHA9N_000259"
     CDS             complement(18492..19208)
                     /locus_tag="ACHA9N_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9083755.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="ACHA9N_000260"
     CDS             complement(19209..21032)
                     /gene="glpO"
                     /locus_tag="ACHA9N_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9083756.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="ACHA9N_000261"
     CDS             complement(21046..22542)
                     /gene="glpK"
                     /locus_tag="ACHA9N_000261"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9083757.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22678..24138)
                     /locus_tag="ACHA9N_000262"
     CDS             complement(22678..24138)
                     /locus_tag="ACHA9N_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9083758.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24128..25423)
                     /locus_tag="ACHA9N_000263"
     CDS             complement(24128..25423)
                     /locus_tag="ACHA9N_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9083759.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25755..26432)
                     /locus_tag="ACHA9N_000264"
     CDS             complement(25755..26432)
                     /locus_tag="ACHA9N_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9083760.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26462..27553)
                     /locus_tag="ACHA9N_000265"
     CDS             complement(26462..27553)
                     /locus_tag="ACHA9N_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9083761.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27654..28088)
                     /locus_tag="ACHA9N_000266"
     CDS             complement(27654..28088)
                     /locus_tag="ACHA9N_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9083762.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28672..30129)
                     /locus_tag="ACHA9N_000267"
     CDS             complement(28672..30129)
                     /locus_tag="ACHA9N_000267"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9083763.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30126..32021)
                     /locus_tag="ACHA9N_000268"
     CDS             complement(30126..32021)
                     /locus_tag="ACHA9N_000268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9083764.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32079..33854)
                     /locus_tag="ACHA9N_000269"
     CDS             complement(32079..33854)
                     /locus_tag="ACHA9N_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9083765.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33904..35400)
                     /locus_tag="ACHA9N_000270"
     CDS             complement(33904..35400)
                     /locus_tag="ACHA9N_000270"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9083766.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35594..37216)
                     /locus_tag="ACHA9N_000271"
     CDS             complement(35594..37216)
                     /locus_tag="ACHA9N_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9083767.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37665..38978)
                     /locus_tag="ACHA9N_000272"
     CDS             complement(37665..38978)
                     /locus_tag="ACHA9N_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9083768.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39223..39447)
                     /locus_tag="ACHA9N_000273"
     CDS             complement(39223..39447)
                     /locus_tag="ACHA9N_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083769.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39467..39889)
                     /locus_tag="ACHA9N_000274"
     CDS             complement(39467..39889)
                     /locus_tag="ACHA9N_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9083770.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40033..40743)
                     /locus_tag="ACHA9N_000275"
     CDS             complement(40033..40743)
                     /locus_tag="ACHA9N_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9083771.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40761..41258)
                     /locus_tag="ACHA9N_000276"
     CDS             complement(40761..41258)
                     /locus_tag="ACHA9N_000276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083772.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41295..41747)
                     /locus_tag="ACHA9N_000277"
     CDS             complement(41295..41747)
                     /locus_tag="ACHA9N_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9083773.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41882..42487)
                     /locus_tag="ACHA9N_000278"
     CDS             complement(41882..42487)
                     /locus_tag="ACHA9N_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9083774.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42937..43557)
                     /locus_tag="ACHA9N_000279"
     CDS             complement(42937..43557)
                     /locus_tag="ACHA9N_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9083775.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43559..44428)
                     /locus_tag="ACHA9N_000280"
     CDS             complement(43559..44428)
                     /locus_tag="ACHA9N_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9083776.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44451..44858)
                     /locus_tag="ACHA9N_000281"
     CDS             complement(44451..44858)
                     /locus_tag="ACHA9N_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9083777.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44877..46166)
                     /locus_tag="ACHA9N_000282"
     CDS             complement(44877..46166)
                     /locus_tag="ACHA9N_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9083778.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46193..46495)
                     /locus_tag="ACHA9N_000283"
     CDS             complement(46193..46495)
                     /locus_tag="ACHA9N_000283"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9083779.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46520..48613)
                     /locus_tag="ACHA9N_000284"
     CDS             complement(46520..48613)
                     /locus_tag="ACHA9N_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9083780.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49102..49866)
                     /locus_tag="ACHA9N_000285"
     CDS             complement(49102..49866)
                     /locus_tag="ACHA9N_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9083781.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49977..50846)
                     /gene="mdcB"
                     /locus_tag="ACHA9N_000286"
     CDS             complement(49977..50846)
                     /gene="mdcB"
                     /locus_tag="ACHA9N_000286"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9083782.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50871..51947)
                     /locus_tag="ACHA9N_000287"
     CDS             complement(50871..51947)
                     /locus_tag="ACHA9N_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9083783.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51971..52564)
                     /locus_tag="ACHA9N_000288"
     CDS             complement(51971..52564)
                     /locus_tag="ACHA9N_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9083784.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52568..54244)
                     /gene="mdcD"
                     /locus_tag="ACHA9N_000289"
     CDS             complement(52568..54244)
                     /gene="mdcD"
                     /locus_tag="ACHA9N_000289"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9083785.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54234..54533)
                     /locus_tag="ACHA9N_000290"
     CDS             complement(54234..54533)
                     /locus_tag="ACHA9N_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9083786.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54635..56281)
                     /gene="mdcA"
                     /locus_tag="ACHA9N_000291"
     CDS             complement(54635..56281)
                     /gene="mdcA"
                     /locus_tag="ACHA9N_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFM9083787.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56288..57199)
                     /locus_tag="ACHA9N_000292"
     CDS             complement(56288..57199)
                     /locus_tag="ACHA9N_000292"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9083788.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57319..58176
                     /locus_tag="ACHA9N_000293"
     CDS             57319..58176
                     /locus_tag="ACHA9N_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9083789.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58363..58725)
                     /locus_tag="ACHA9N_000294"
     CDS             complement(58363..58725)
                     /locus_tag="ACHA9N_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9083790.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58914..59096)
                     /locus_tag="ACHA9N_000295"
     CDS             complement(58914..59096)
                     /locus_tag="ACHA9N_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083791.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59071..60510
                     /locus_tag="ACHA9N_000296"
     CDS             59071..60510
                     /locus_tag="ACHA9N_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9083792.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60517..63069)
                     /locus_tag="ACHA9N_000297"
     CDS             complement(60517..63069)
                     /locus_tag="ACHA9N_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9083793.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63183..64514)
                     /locus_tag="ACHA9N_000298"
     CDS             complement(63183..64514)
                     /locus_tag="ACHA9N_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9083794.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64480..65958)
                     /locus_tag="ACHA9N_000299"
     CDS             complement(64480..65958)
                     /locus_tag="ACHA9N_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9083795.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66263..68098)
                     /gene="tdc"
                     /locus_tag="ACHA9N_000300"
     CDS             complement(66263..68098)
                     /gene="tdc"
                     /locus_tag="ACHA9N_000300"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9083796.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19420 a        14578 c        11697 g        22622 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atccgcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     7201 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     7261 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     7321 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     7381 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     7441 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     7501 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     7561 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     7621 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     7681 agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     7741 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     7801 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     7861 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     7921 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     7981 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     8041 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     8101 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     8161 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     8221 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     8281 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     8341 cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat cttcaacgtc
     8401 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     8461 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     8521 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     8581 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     8641 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     8701 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     8761 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     8821 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     8881 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     8941 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc ttcgatcaca
     9001 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     9061 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     9121 catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata gacaaacaag
     9181 tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca ccccattttc
     9241 tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga cagaataaaa
     9301 acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt cttcaagtaa
     9361 ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta cttttttgta
     9421 tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat
     9481 caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa aagcgtacaa
     9541 ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata taaaaggaag
     9601 aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta accagtaatt
     9661 accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc ttgttacttt
     9721 tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat acatttcatt
     9781 tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac atttctcatc
     9841 agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt tatctcttaa
     9901 ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg ccgtgtacat
     9961 ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga ttacatgatc
    10021 aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta atccatatga
    10081 atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg tgataactgc
    10141 cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca attcttcaga
    10201 ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata cgatgtcgat
    10261 cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc tagaacggta
    10321 actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac aacgcataat
    10381 atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa
    10441 gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat aatcttgtac
    10501 ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg
    10561 aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag cataagctga
    10621 gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc atgccatttc
    10681 ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg cataagctac
    10741 aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact gcctatcttc
    10801 tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca aaagatgatt
    10861 cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca atttaggatg
    10921 agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg tttttccatt
    10981 tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg attgttttag
    11041 attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat tttctcccgt
    11101 gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct gtgaaaccag
    11161 ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga cattcgaaat
    11221 atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg tcatcgtatc
    11281 tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt taccacttcc
    11341 tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg tgttttgttc
    11401 cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc ctaagacttc
    11461 taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca aacgatctct
    11521 ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt gacgaaccgt
    11581 ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg aacgccaatc
    11641 ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat aaaaggattc
    11701 ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac ttttcaataa
    11761 acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca tctgatcgat
    11821 ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa
    11881 attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta tttttgccgt
    11941 atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca cgttcttcag
    12001 atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct ttttttaaag
    12061 tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt aacgagtatc
    12121 atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa aatgcaccat
    12181 caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga tagctacgta
    12241 atccgctatg acagctgacg atatattctt gttggctgtc taattcattt aatcggtccc
    12301 gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc aattcttctg
    12361 ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat tcatgccatt
    12421 gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc atgtttactg
    12481 gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt aaatcgaaga
    12541 tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc acaccttttg
    12601 ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc aacttcagta
    12661 aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca tgaatgactt
    12721 caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat gcggcagtca
    12781 ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga gaaattccgg
    12841 aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga gaaataagtg
    12901 catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca taaatatcag
    12961 gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct aattcgattc
    13021 cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata actaaatcag
    13081 cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca aatccagcga
    13141 cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct ataaaagctg
    13201 ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc atgacttcta
    13261 aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca ccaacgatca
    13321 cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct ggtacatttc
    13381 taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca actggttttg
    13441 caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc tcatgtacga
    13501 cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga cgcacatcta
    13561 aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc cgttcactga
    13621 tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac ggtccttttt
    13681 caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca gcagacattc
    13741 cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac tgttcgatca
    13801 aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt ctttaattct
    13861 ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt cttttctttg
    13921 tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt tatatgcccg
    13981 ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa aagcttttcc
    14041 tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat tttctgcacc
    14101 tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt attaatatgt
    14161 caaccagaat gactcttcat ttttttcact actttttata tttgcataaa ataccgataa
    14221 ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga taataaaaat
    14281 atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa aatgatcgaa
    14341 gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc tagtattgat
    14401 cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa acctgatagt
    14461 aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat taaaaagtaa
    14521 aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac
    14581 agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg tcgtatctgt
    14641 aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct tagatttgca
    14701 aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt cagaaagttc
    14761 tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg aactgcaatc
    14821 ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag tgatcagctt
    14881 caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt
    14941 gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat
    15001 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    15061 ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa atagctcctc
    15121 atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc gtttaaggaa
    15181 gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga aaatggattt
    15241 ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat accgtttaag
    15301 gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagc
    15361 tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
    15421 ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt caaaaacaac
    15481 ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg agtctattta
    15541 tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga aacatctttc
    15601 ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa
    15661 tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc atgccaacat
    15721 agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg atatttttag
    15781 atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat agaccaaaca
    15841 acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt
    15901 tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat ctccgagcaa
    15961 tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct gctatataaa
    16021 atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg aatatcgata
    16081 aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc attttatcgc
    16141 cctacctcct cagcactctt ttctttttat tttagcttac acggggaata aaaccaatga
    16201 tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt gagataatca
    16261 cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt cttttactag
    16321 tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac gtgttttata
    16381 atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac
    16441 cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata catcaatcgt
    16501 atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga caaatatccg
    16561 atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg taactgctgt
    16621 cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt aacgctctgc
    16681 ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag cttggctttc
    16741 tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat cgaaagccat
    16801 agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc cataatgggt
    16861 cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga tcgctagtgc
    16921 agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca acgtattcct
    16981 ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc
    17041 attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga cagcaagctc
    17101 tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca ataaaccgac
    17161 tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca agcctcgata
    17221 gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc
    17281 ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat tgatttctcg
    17341 gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga tcaataaatc
    17401 gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt gatattcttc
    17461 actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa tcacagttgg
    17521 ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat ctattttatt
    17581 tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa tcattgcttc
    17641 ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg actggatttc
    17701 tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact
    17761 gactttatta ttttttattt tctggtcaat caactcttct acatttatca aatcaggaag
    17821 atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa aaaactttgt
    17881 gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg gctgcagaat
    17941 caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca taaatggaac
    18001 atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac
    18061 agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat agtttccaat
    18121 tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa tcatttcgtt
    18181 gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa gggcctagag
    18241 ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat tagaatacaa
    18301 agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact ttgtttattg
    18361 gctattcgac ttactgatta gataccagct acactcacaa aacatacccc attcttctag
    18421 gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa actttagtat
    18481 gttttataga tttacaccat taatgaatag acacctaccg cgataattgc tccgatcatt
    18541 ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt agctattggc
    18601 aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc atatcctgtt
    18661 ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa tacgttcgtt
    18721 ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac aaatgtacca
    18781 attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt tgcaaacgtt
    18841 cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga aagccaaaca
    18901 agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac gacaagattc
    18961 caactaattg cccctgtcat cgccatagct agagagactg caggattcaa atgagctggg
    19021 cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc aaatgcgatg
    19081 acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc acacacacca
    19141 tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg agtcatatca
    19201 ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat caatgcttct
    19261 aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc agcaattgca
    19321 tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag atgatttgta
    19381 cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc ataacgtaac
    19441 cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc taattcaaag
    19501 atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc ttcctctaaa
    19561 ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt agttggatcg
    19621 aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc tattccgtat
    19681 tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt acggtaatcg
    19741 gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa agaactacct
    19801 ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt caacacgtct
    19861 tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc gacaacttta
    19921 ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga accagaatta
    19981 ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa tgtgatattt
    20041 gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc ttgtgtgact
    20101 ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta agtcttgttt
    20161 tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa gtatgtgggt
    20221 tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt tggacgcatt
    20281 ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca aggaccactc
    20341 gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag gtcacgagct
    20401 ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat tttacttact
    20461 agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg ggcatcattg
    20521 ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa aaacggttct
    20581 ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc tgtcacattt
    20641 gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa ggttgttgct
    20701 ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg tggggcaata
    20761 ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc aaatgtcttc
    20821 agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa
    20881 tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac tgccacaccg
    20941 gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg cattttttga
    21001 atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt caaatgcttg
    21061 ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct cttcctccat
    21121 ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg tatctttcca
    21181 ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc
    21241 tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat attcattgtt
    21301 tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt
    21361 atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa tgatatcttc
    21421 ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat cccaataagg
    21481 agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg tagagcgttt
    21541 cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc tcagccattg
    21601 aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct ttccatttat
    21661 gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt caccggtatt
    21721 catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt cgaatgccat
    21781 ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg cttcgcttcc
    21841 ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga cttttggtaa
    21901 catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta gctgatggat
    21961 attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt gtccatcggt
    22021 cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt tttcagctcg
    22081 ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg agaaataggc
    22141 atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat tttttaagtt
    22201 atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga ttggacgtcc
    22261 tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa tcccagcaat
    22321 ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact gtacagaatt
    22381 ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt gcgtgaactc
    22441 tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc gtgagctagt
    22501 tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc cctccattat
    22561 atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa gaaactgtca
    22621 aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat tttttaatca
    22681 attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt cgtaaggtga
    22741 cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa atgtgatcac
    22801 caaatcatat tctttacctt cttcataact tccaatctca ataccgccga ttcccctcag
    22861 ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt ctaaatcgat
    22921 tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat tggcatactg
    22981 gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt gctgcaccgc
    23041 tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt tctgctgctg
    23101 ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa accattcatt
    23161 gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca aacgacgagg
    23221 acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg ttggtaactt
    23281 tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg aaaaagaaac
    23341 aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg ttcgactaag
    23401 atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat agagtaattt
    23461 cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt tcgtaatccc
    23521 gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta atacacggtc
    23581 caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc
    23641 ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct cttctccgta
    23701 taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca attctttgat
    23761 ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa cgatggatac
    23821 ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct gcaataaaaa
    23881 caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct gatactcatt
    23941 tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat cttccaaata
    24001 ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga tagaggctgt
    24061 tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt ttctttctaa
    24121 taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata tctggcacac
    24181 ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc catccttctt
    24241 ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt tggagctgtg
    24301 ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg ctttgattta
    24361 ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa aaaatgctct
    24421 cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg ccgactggac
    24481 gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca tttcgtgctg
    24541 tacggacagc gttatttact agagaaacat acattttttc atttgttgga cgaaaagtta
    24601 cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag tagtcatcta
    24661 cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga ttgtcggggc
    24721 gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc gtctctacaa
    24781 tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt gaaagccctt
    24841 gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa cgaaaaagaa
    24901 ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac ccttcagcta
    24961 tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc atgatctttt
    25021 gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt gattctgcag
    25081 aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca aaatccaatt
    25141 tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc ttttctgtgt
    25201 aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa agttgttttt
    25261 cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat cctaacttcg
    25321 attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga taaatagccg
    25381 catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt cctctttctt
    25441 ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa ctcgctttca
    25501 accttatgat acatagcgat gcagcagaat caaccctctc catcataccg tgttctaggt
    25561 ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg aagagatgtt
    25621 tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc tgtccaaata
    25681 ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag ttagaataga
    25741 cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa ttgatgagca
    25801 gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc tgaaaaagct
    25861 gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa gaatggtact
    25921 tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac tgtacgtcca
    25981 gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac accgtgacaa
    26041 acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc aagatctatt
    26101 gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc tgcataatca
    26161 tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct gaaatcttca
    26221 tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg ttctaaaggt
    26281 acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac taatacatca
    26341 taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt ttcgtacttt
    26401 tctacatttg ttaatacgat caatactttc attttcccat cctctttcta taaaaattgc
    26461 gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa agctctttat
    26521 atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat aatttctctg
    26581 cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca tatttcaata
    26641 cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg atctttcctt
    26701 gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca gaacgagcta
    26761 ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca cgtaatcgtt
    26821 caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct tctggtgaga
    26881 aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag aaaaccgttg
    26941 ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat tgatcctctc
    27001 gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct ccatgcaact
    27061 cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat gcttggattg
    27121 tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga cgttttgctg
    27181 caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt gtcattcgtc
    27241 caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct ttcgctagct
    27301 cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc cagccttttc
    27361 cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta gaacgttgtt
    27421 tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca
    27481 taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta attgattgat
    27541 caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc gagaatcatc
    27601 tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa attttatttt
    27661 ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact tgcttcattt
    27721 cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac ttccttagca
    27781 aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct aactgttttg
    27841 acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag
    27901 agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat caataaaata
    27961 aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa tttattcaac
    28021 atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt ttcaattggc
    28081 ttttccattt catcccctcc attggcgtac atatcatagt atgattttta ttttttgtca
    28141 aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa gtagccatct
    28201 tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt
    28261 gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat
    28321 ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt tatatcagag
    28381 gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    28441 tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg tttattgaga
    28501 ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa acagcttctc
    28561 atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac tgtttattcg
    28621 taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt attataggat
    28681 ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct ttaaccgttt
    28741 ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac gttttgacat
    28801 ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac caaataaatc
    28861 tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat agtcaatgcg
    28921 gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga gtccgttttc
    28981 agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa ttctcaaacc
    29041 cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt taattcctcc
    29101 agttattaag tttccttgga ccccttccca attttctgga tgagcactga ctgcactaga
    29161 catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt cttcatcacc
    29221 tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat acgctggata
    29281 atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct caattgtttt
    29341 ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga tcatacaacc
    29401 gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac ttgctataaa
    29461 ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt cttcaattac
    29521 tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag tcatccaata
    29581 ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat
    29641 aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca tttcataatg
    29701 ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca cttgatcata
    29761 aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca
    29821 tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga tgtcttcttt
    29881 aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc tgttgggatc
    29941 ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc atttttcttt
    30001 aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt catctacatc
    30061 ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc cttctggaaa
    30121 ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt tttcttatga
    30181 ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt ggtcggatca
    30241 tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc tttacgtact
    30301 ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac tgtatcaata
    30361 ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc atgtttcgtt
    30421 ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc cgttggaatg
    30481 atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac ttcagataaa
    30541 tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga ttcttcttcg
    30601 atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat cgctaagaaa
    30661 taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga caaaatactt
    30721 cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc aaatatagct
    30781 ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc ataaacagca
    30841 ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact ttttactttc
    30901 ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc tacggcagtc
    30961 atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt cattactggt
    31021 acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc gataacagca
    31081 ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat cagctgactt
    31141 gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac gatgatacaa
    31201 atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca
    31261 atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt ataccttgct
    31321 agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc actgacaatc
    31381 gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt attgaacttt
    31441 tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc agctgctgca
    31501 ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt acataaggcg
    31561 agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc aaaaacagcg
    31621 gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct ctttccacta
    31681 gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc atttactgca
    31741 tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac accttctagt
    31801 tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg taaccttgtt
    31861 gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac aatatctttc
    31921 gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt agcaatcctt
    31981 cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt accccttata
    32041 atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa atcactcact
    32101 gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc
    32161 ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt gatttttgtt
    32221 tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg aagcataact
    32281 aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg gatttttatt
    32341 tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc ttcaactatg
    32401 gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc tagaacacta
    32461 ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt caaaattgca
    32521 tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc tatccggcat
    32581 tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt caaaatacta
    32641 attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt caacgcttcg
    32701 gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg aaaatgtatt
    32761 gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat cttttgtaca
    32821 aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt gatatgggtt
    32881 agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa acgaataatc
    32941 gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc attaatcatc
    33001 aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt ggaaaaagaa
    33061 tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat aaataatgta
    33121 tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac gagataccga
    33181 gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag aacctctttt
    33241 aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg ttgaggcaac
    33301 cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct ttttgaattt
    33361 tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa tttaggattg
    33421 cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt tgaaagaaag
    33481 aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa gtacgtcaca
    33541 atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac ctcttgtatg
    33601 atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg tttatttgac
    33661 tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt ttggatcgtt
    33721 ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg attcttattt
    33781 cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt acacctcctc
    33841 tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc agaggtttaa
    33901 aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga taatttgttt
    33961 ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat cattttgtct
    34021 atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg atacgatatc
    34081 gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg cctcagcaat
    34141 ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc gattcaattc
    34201 atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta attcataacg
    34261 gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc aactccaagg
    34321 ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc cagtatctac
    34381 gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg caatataatc
    34441 tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg cacagtcttc
    34501 ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga catcggagaa
    34561 aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat cacagtcata
    34621 tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat ttggatcgat
    34681 tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac aggcttgata
    34741 aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag ggaatgtaac
    34801 caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa aggtcatcca
    34861 atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc
    34921 gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg gggtttcata
    34981 atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga ttagtctgtc
    35041 ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa atattcgtgc
    35101 ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac gaatatcctc
    35161 ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg attcgtctaa
    35221 agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct ggttcatgat
    35281 cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg caccttcaca
    35341 ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt cctgatccat
    35401 aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat tcatcaaaac
    35461 aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac tcttcatttt
    35521 taatatttat atataacaaa tacataagac gatccccgaa agaaaacact cgttaaccat
    35581 ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt aaatcaactt
    35641 tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa gcttcacgtg
    35701 gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat tgctggtctt
    35761 catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat tcatagtctc
    35821 cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac atataaaaaa
    35881 cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt ggattgactc
    35941 gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat tgcataggcg
    36001 tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct tttgtaaatc
    36061 cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct tccgcgatat
    36121 catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa ggggtgcctt
    36181 tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac acatcgtcat
    36241 ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa ctattccatc
    36301 cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca gagatactca
    36361 agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg tgttcgaatt
    36421 ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg atttcggtag
    36481 ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt gcttgattca
    36541 tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta tccggttctt
    36601 tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg atccagaaat
    36661 tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa tcaggttgac
    36721 gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc caagcagaac
    36781 cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca cgccaaatat
    36841 aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac ggatgttcat
    36901 cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc tttgcttctt
    36961 caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct tgataattac
    37021 tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc caaattgcat
    37081 caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa tctccaatac
    37141 catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact gaatggtgcc
    37201 accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt tgcttaatca
    37261 tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt gcaccttgtt
    37321 atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa
    37381 ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac ggtgttcact
    37441 cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat tttttcgttt
    37501 ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa cggtgttcac
    37561 tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt
    37621 tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt cacattcaat
    37681 atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga tacttctttt
    37741 cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc aatcccatct
    37801 gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat cgcaatctct
    37861 aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac cataatcaat
    37921 gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa caagcctaac
    37981 aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac ggcgatacca
    38041 gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc cgcaagcata
    38101 gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc tttcatgaaa
    38161 cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt cacaagcaag
    38221 aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg ttttaatgca
    38281 actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg cattggaatc
    38341 aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat gccacctggt
    38401 acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa ccaagtagaa
    38461 ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat gatcccacct
    38521 tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc atatttcaaa
    38581 tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat caaaataaaa
    38641 atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc cattcctaaa
    38701 caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc aattggataa
    38761 cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt cgcagtaaac
    38821 actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa taagatataa
    38881 gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt ggtgttcaat
    38941 ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc taaatccgaa
    39001 cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg ttttatcata
    39061 tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc
    39121 atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt acaagaattt
    39181 ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt gtgatttgat
    39241 tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag gattcagatt
    39301 atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct tcttttttcc
    39361 tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat acttcaaata
    39421 accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa tcagacgact
    39481 cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa gtcaagatgc
    39541 cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt tcaaattcat
    39601 ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa
    39661 atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca atactcttaa
    39721 attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct acaaagaatc
    39781 ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt atcatcattt
    39841 gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt atatcacgaa
    39901 agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt ttctaggatt
    39961 gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc cgctcttctt
    40021 atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact
    40081 agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg
    40141 tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt
    40201 cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc
    40261 atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa
    40321 tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc
    40381 tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt
    40441 gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg
    40501 tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt
    40561 ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa
    40621 ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa
    40681 gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt
    40741 catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga
    40801 tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa
    40861 gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct
    40921 tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt
    40981 tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc
    41041 gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa
    41101 tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag
    41161 taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata
    41221 ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc
    41281 acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg
    41341 ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa
    41401 agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt
    41461 tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa
    41521 cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct
    41581 actttgatca tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact
    41641 aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa
    41701 tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt
    41761 tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat tttcaataaa
    41821 aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag
    41881 ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc
    41941 gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat
    42001 tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat
    42061 actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag
    42121 tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag
    42181 acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga
    42241 ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat
    42301 gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac
    42361 tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt
    42421 ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct
    42481 tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga
    42541 ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa
    42601 aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc
    42661 ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc
    42721 tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa cggtgttctc
    42781 ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct gtttttttga
    42841 aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt aaacagcgtt
    42901 caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat tgatttcttt
    42961 ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta catcaaattc
    43021 atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct ttttgaatgc
    43081 ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac catagtgata
    43141 atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt accgacgttg
    43201 acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc cgtatctatc
    43261 tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg acacaatcac
    43321 tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat aaactttcgt
    43381 cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg catagtattc
    43441 cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta gtttattcat
    43501 aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact ttttcatctc
    43561 atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc ttatgcttga
    43621 caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac ttgttgattt
    43681 ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc tccgttccaa
    43741 cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct ttcacacctg
    43801 ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc agcaagggaa
    43861 tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca atagctaatg
    43921 catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta tataccttgt
    43981 cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt tctccttcta
    44041 cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg atattttctt
    44101 tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg attgcttctt
    44161 tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc atttcttctt
    44221 ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta gcggtatcga
    44281 cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa
    44341 gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct tctgctgttt
    44401 ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt ttatttgttt
    44461 cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc catgtagtaa
    44521 tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt atagaggctc
    44581 atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt ttctaaatat
    44641 tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg ttcataaagt
    44701 ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg tgtaatctct
    44761 gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc cagaactttt
    44821 ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac tttcaattaa
    44881 aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc tactgtatct
    44941 acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac tagcaaagcg
    45001 ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc tcggcgtccg
    45061 ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt tagcatacca
    45121 ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat cgtaccaatc
    45181 gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa aactggacaa
    45241 tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa tgccggcacg
    45301 atttcaccta acagtagacg tacaccagct agcaatacat acacacccac tacgaattgg
    45361 atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc agaagcgaat
    45421 ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac catgacagac
    45481 aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt
    45541 ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa gtacccgatc
    45601 gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat gatcggttgc
    45661 gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac caagatcaat
    45721 acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc catccataaa
    45781 attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa aataatattg
    45841 aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa atcattcatt
    45901 gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa tccttcagta
    45961 aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc actcaacact
    46021 tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga tttcttctgc
    46081 agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt caaaatattt
    46141 tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt atttagataa
    46201 ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt gaaacaatat
    46261 ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac tgcgaacgaa
    46321 aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa atatcacaat
    46381 ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac atctgtgctg
    46441 caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg ttcatctttg
    46501 ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt ttgaaatctt
    46561 cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc aattcaatta
    46621 gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa aaaataatct
    46681 ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta gctacgctga
    46741 ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg tgtttgccaa
    46801 tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca tagcgctctt
    46861 cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc tcccaagtag
    46921 tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac ataggctgga
    46981 tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac ttgccataga
    47041 tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga gtgaataact
    47101 ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt ttcaaaaagt
    47161 cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa agcggcttat
    47221 ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta tctactaatt
    47281 cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt tctttcagat
    47341 tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact gatttataaa
    47401 cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg acaagaaaag
    47461 ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga acaaaatgag
    47521 tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat ggattcatga
    47581 cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag agattttcga
    47641 ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca aattggatca
    47701 attggatcgt tagtaattga gcttgtaccc actcgattga attggagata tctctagcat
    47761 tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca ttgtccaatt
    47821 gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt atttctgtat
    47881 ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc cagatcaatg
    47941 taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac ttattggaat
    48001 agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag attttttgag
    48061 ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga
    48121 taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct
    48181 ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat
    48241 cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg
    48301 ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa
    48361 agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct
    48421 tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc
    48481 agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg aactcacttg
    48541 ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg
    48601 ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa
    48661 taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa
    48721 taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat
    48781 tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
    48841 caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta tcgcagatgc
    48901 aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc ttgagcgata
    48961 agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag
    49021 gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac
    49081 tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat cggcttgcca
    49141 tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt
    49201 cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa
    49261 atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat
    49321 agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg
    49381 atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc
    49441 ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg ctttcttttc
    49501 tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct tttgtctttt
    49561 attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa
    49621 tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat aagaatctct
    49681 gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa atgaacctct
    49741 gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg
    49801 ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt
    49861 tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat agccttgcta
    49921 aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg tcttatttag
    49981 tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc tatcttcagc
    50041 atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg gttttcctga
    50101 caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact agcagcatga
    50161 gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac gcatgtgtca
    50221 tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata tgaatcatta
    50281 tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa tatcgcttct
    50341 tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt ggttttttta
    50401 tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag tattttcgat
    50461 aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa tagccccatt
    50521 gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat tgccttttcc
    50581 gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc atgatgccat
    50641 gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt aagaaataaa
    50701 gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc aggttttgga
    50761 cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg ctgagtcact
    50821 gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta ttacttgctg
    50881 aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc aaatgcacaa
    50941 cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg cagccaacta
    51001 tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata cgtacgataa
    51061 gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac aatcattttg
    51121 gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg gatcgtattc
    51181 aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga acggacagaa
    51241 aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc caaatattga
    51301 tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact agcaacaaac
    51361 cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt ttgatagaac
    51421 agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac atatttttta
    51481 ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg gatctgcagc
    51541 atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt cagtaaaaag
    51601 cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca aagataagcc
    51661 gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa aaattcccaa
    51721 taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat aaacattggt
    51781 gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt tgcgctgtgg
    51841 ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac agctgatact
    51901 ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc tttctctctc
    51961 tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt ttcaatagaa
    52021 tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc cgagtcacta
    52081 cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga attggcaatt
    52141 caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt actgccggaa
    52201 tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat cctgataata
    52261 tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc tcttgtctat
    52321 acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca tgttttgaga
    52381 tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt agtatttctg
    52441 cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga acgacccatt
    52501 caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca tgtgcttgaa
    52561 tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt gtttcgacac
    52621 gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg tcatttgtat
    52681 tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca ttttgttctg
    52741 aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt tttgtataat
    52801 tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt tcagcaaccg
    52861 aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta atttctttat
    52921 tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca cctgaaatag
    52981 catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt gcataagcag
    53041 cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag ccgtatgctt
    53101 ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct ttatcttctt
    53161 cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca acttcaccgt
    53221 ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg atggcttctt
    53281 ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt ggatttttca
    53341 tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt tcatttcctt
    53401 ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct tcattgtaat
    53461 cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa taaaatgctg
    53521 agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca gcagctttga
    53581 ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc cgttgacgag
    53641 atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga acgcctgctt
    53701 cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt gcgattaaat
    53761 aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt cctggaatca
    53821 aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt tctgaaatag
    53881 ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt acaatgactg
    53941 gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca gcaacgattt
    54001 ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata gcgattacta
    54061 ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca ctttcaggga
    54121 cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca atcaattcac
    54181 gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca aaactatttt
    54241 tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc tggtgtagca
    54301 ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa tctcgttaat
    54361 acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac agtagattct
    54421 gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac tcctacatgg
    54481 attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc catggatctc
    54541 tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt cttccatctg
    54601 ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg aatttcattg
    54661 gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt gcagctaata
    54721 atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc acgatgccgg
    54781 ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt gtaacaccgc
    54841 caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac aaataagcaa
    54901 ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact ggtgcattgg
    54961 ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag actggttttt
    55021 tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc cctaatggat
    55081 catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg ccaggattat
    55141 gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg gtagaagagt
    55201 ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca tattgtcctg
    55261 cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca aaaaagacat
    55321 ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta tggatgcttt
    55381 caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat acccagttct
    55441 tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac aattcgatag
    55501 ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct gcgtagattc
    55561 ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga ggatctcgtg
    55621 tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct acccagtcac
    55681 cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg atcacgatac
    55741 catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg aaacctgtgt
    55801 acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt ggaatcaaat
    55861 cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat ttcattgtgc
    55921 cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag aaatcgatct
    55981 tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata ctagacatga
    56041 tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct gaaagaaaac
    56101 ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca atcagttttt
    56161 ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag ttgctgattt
    56221 tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt ggtttttcca
    56281 taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca tgttggtcta
    56341 gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc tttgtcaaag
    56401 tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc aagtccatca
    56461 tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga atctcttctg
    56521 cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca tcagatagat
    56581 ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag agacagtgag
    56641 atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg gcttctgcca
    56701 acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga caattttcat
    56761 ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc tcagcttcgc
    56821 ttctcgtcac acctactact acgcccattg cataaccgct tggataagct tctttcatta
    56881 agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat cgacaagcaa
    56941 cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga ataccgtttt
    57001 tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg ataaaaaccg
    57061 tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt tctaaacgtt
    57121 ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct tgtccaggaa
    57181 ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc actgctatct
    57241 tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa caaaaaggta
    57301 tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa agtattcgaa
    57361 acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc cttgagccgt
    57421 gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag cggcagaaac
    57481 attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac attagacaca
    57541 ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac ttccgtcata
    57601 ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt tatggaaaag
    57661 catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat taccgatcct
    57721 gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat catgaaagaa
    57781 cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca tctgcaatta
    57841 gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt ccgtcataca
    57901 ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac gacagatgat
    57961 cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt cccaaaagtt
    58021 tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga tttgcccttg
    58081 acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat caaaatgatt
    58141 gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg ggacaaaatt
    58201 cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt agaaaataag
    58261 ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    58321 taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg ttgatttaaa
    58381 acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta gatcaataga
    58441 gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc ctcgcaaaat
    58501 tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat ggtcacttgc
    58561 atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt ttttgctttt
    58621 ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg gagacaaatt
    58681 gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct tccatctctt
    58741 tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga atacaagtag
    58801 ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt tcaatccgcc
    58861 tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc cacctatttt
    58921 ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc agtaaatgct
    58981 ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt tattttctcc
    59041 gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa aatcattacc
    59101 gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca tacaaagata
    59161 accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt ttctgacctt
    59221 caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca agcaggaatc
    59281 agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt tttcaaacaa
    59341 tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc tgtccctgat
    59401 tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac taaaatcaat
    59461 cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc ttgtattctt
    59521 ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt cactcataac
    59581 aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt tttaagaaac
    59641 ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg ttggttcttt
    59701 atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca taccaacagc
    59761 acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga aggtatttat
    59821 cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg gaattacaac
    59881 aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt agaggtcctc
    59941 gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa aaaaacatct
    60001 ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat attgattgaa
    60061 gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata tgcacacttg
    60121 tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat caatgaacca
    60181 aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc tatcaaaccg
    60241 aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc gttcttgcag
    60301 caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga actaaccgat
    60361 ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc tctagattct
    60421 ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct caatcgtttg
    60481 actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt ctctagctgt
    60541 tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta caactaagaa
    60601 tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac ttactgtacc
    60661 gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag caaggatacc
    60721 taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg gcttggacaa
    60781 ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca gaagaatggt
    60841 ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa ccatgatcag
    60901 tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt gctttggatc
    60961 tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg cataagataa
    61021 agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag gtagcgggcg
    61081 attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg gcaagaagaa
    61141 attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg cttttgcaat
    61201 cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc
    61261 tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat catttacgcc
    61321 gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa ttcgcagttt
    61381 atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa tcaactcttc
    61441 atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt ttaagccaat
    61501 ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt tcactgagat
    61561 ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg gatcgatcaa
    61621 tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga
    61681 atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa attctgcagc
    61741 tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa ataatacttc
    61801 tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg ttgccatata
    61861 tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta gattggctgt
    61921 ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt ctgttttctg
    61981 ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc aggcttcttc
    62041 ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg tcttatctga
    62101 acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga tgatcgcctt
    62161 ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag ctggcaatcc
    62221 ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat gccaatccat
    62281 accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa tgccttgaaa
    62341 aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt gttcgaccga
    62401 ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc
    62461 gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct ggtcgccgat
    62521 cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt ctcccgtcaa
    62581 aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg ctggaacgac
    62641 atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag ggattttttc
    62701 ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa
    62761 tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga tcactacgaa
    62821 taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag aggcaatgat
    62881 caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc gttttgtctg
    62941 ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc gttcttcttg
    63001 cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg tcaatctgta
    63061 ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag ggaagttttt
    63121 tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa atgtatctgg
    63181 gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa tacaaaccag
    63241 ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct gaaagcaatt
    63301 gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt ccgtttcttt
    63361 tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag aaaacaactc
    63421 gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa aaaatgtaga
    63481 caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg tacgcctttc
    63541 ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg atataaaatc
    63601 ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa aatcattaga
    63661 ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac taaaggactt
    63721 tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat cgttcctata
    63781 tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt cttttgcgga
    63841 tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact gaataaaacc
    63901 ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc gatctgtcca
    63961 aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa tcctataccc
    64021 aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga ttccaattca
    64081 ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc gctacctccg
    64141 cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat tgctgttaat
    64201 tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa aaatgcccac
    64261 tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact atttgctaaa
    64321 aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat caaataaacg
    64381 agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa taatcctgtt
    64441 ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt ccgtttcatt
    64501 gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga cttcctgttc
    64561 ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa tgacactgat
    64621 ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc cgacagaaaa
    64681 tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc tctttttctc
    64741 tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca atctcaaaac
    64801 tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat ttacgagcat
    64861 caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga ttgagataat
    64921 gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct tccaaaaatt
    64981 gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg ctggtcctga
    65041 ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa acagattcac
    65101 aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg cgatattatt
    65161 caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca aaatattgac
    65221 gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa ctggattttc
    65281 caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca tgatcggcgc
    65341 aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc atgaaaaact
    65401 gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc ctaataagag
    65461 catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc cgatttttgc
    65521 catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa tgacaactaa
    65581 tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa acaacggagc
    65641 gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca gccatgagac
    65701 aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac cacctttttg
    65761 gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt acaataatac
    65821 tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg gaatatttcg
    65881 tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga tttgctttga
    65941 tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt tttgttttct
    66001 tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca aaacccagta
    66061 tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga aacgaacgat
    66121 ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat gaaaaacagg
    66181 gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg tctcttgtta
    66241 ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt tccattgctt
    66301 tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa ggagtcatga
    66361 tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc gttagaccca
    66421 ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat gtagtatgag
    66481 aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga tcgaacattt
    66541 tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg aatacccaat
    66601 tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt cctttaacag
    66661 taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt gaagccccaa
    66721 tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc cagactgcgg
    66781 cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata tctggagcct
    66841 ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc atatttttat
    66901 gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga ggatctatcg
    66961 tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg tcaatcgtta
    67021 cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca tacggcacaa
    67081 attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg gcatcaacat
    67141 gtaaatagaa atagatccct tcatctttta acctttctct taattggacg attttatcta
    67201 cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct agaattggga
    67261 ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc attcgataat
    67321 cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata tctaacgcct
    67381 tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt tggatatttt
    67441 ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta aagctttcgg
    67501 tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac atttttttca
    67561 ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa cgggcgtacc
    67621 ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat ccgtctgtca
    67681 tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa gttgccatag
    67741 aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa gctaataaag
    67801 caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa tgccatggga
    67861 tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata tggtcgcggg
    67921 tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata tctgaaggaa
    67981 tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac attttataaa
    68041 ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata tctttcattt
    68101 ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta tctgtaacac
    68161 ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat ccacgtagat
    68221 ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca aaaaagcgaa
    68281 caatttttca ataaatcagt cttctaaaat agataac
//
LOCUS       DAYYJC010000005        60875 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000005 DAYYJC010000000
VERSION     DAYYJC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.005"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="ACHA9N_000301"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="ACHA9N_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9083797.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="ACHA9N_000302"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="ACHA9N_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9083798.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA9N_000303"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="ACHA9N_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9083799.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA9N_000304"
     CDS             4744..5628
                     /gene="ftsX"
                     /locus_tag="ACHA9N_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9083800.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="ACHA9N_000305"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="ACHA9N_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9083801.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="ACHA9N_000306"
     CDS             7565..8422
                     /locus_tag="ACHA9N_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9083802.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA9N_000307"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="ACHA9N_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9083803.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA9N_000308"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="ACHA9N_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9083804.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA9N_000309"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="ACHA9N_000309"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9083805.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA9N_000310"
     CDS             11178..11936
                     /gene="pstB"
                     /locus_tag="ACHA9N_000310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9083806.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA9N_000311"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="ACHA9N_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9083807.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13203..13895
                     /locus_tag="ACHA9N_000312"
     CDS             13203..13895
                     /locus_tag="ACHA9N_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9083808.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            13892..14659
                     /locus_tag="ACHA9N_000313"
     CDS             13892..14659
                     /locus_tag="ACHA9N_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9083809.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA9N_000314"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="ACHA9N_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9083810.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="ACHA9N_000315"
     CDS             16624..16944
                     /locus_tag="ACHA9N_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9083811.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="ACHA9N_000316"
     CDS             16944..17294
                     /locus_tag="ACHA9N_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9083812.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA9N_000317"
     CDS             17461..18399
                     /gene="hprK"
                     /locus_tag="ACHA9N_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9083813.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA9N_000318"
     CDS             18414..19244
                     /gene="lgt"
                     /locus_tag="ACHA9N_000318"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9083814.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="ACHA9N_000319"
     CDS             19288..20313
                     /locus_tag="ACHA9N_000319"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9083815.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="ACHA9N_000320"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="ACHA9N_000320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9083816.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="ACHA9N_000321"
     CDS             21391..21831
                     /locus_tag="ACHA9N_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9083817.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="ACHA9N_000322"
     CDS             21834..22436
                     /locus_tag="ACHA9N_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9083818.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="ACHA9N_000323"
     CDS             complement(22495..23601)
                     /locus_tag="ACHA9N_000323"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9083819.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA9N_000324"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="ACHA9N_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9083820.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="ACHA9N_000325"
     CDS             complement(25149..27494)
                     /locus_tag="ACHA9N_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9083821.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="ACHA9N_000326"
     CDS             27773..28675
                     /locus_tag="ACHA9N_000326"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9083822.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="ACHA9N_000327"
     CDS             28888..30663
                     /locus_tag="ACHA9N_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9083823.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="ACHA9N_000328"
     CDS             complement(30729..32315)
                     /locus_tag="ACHA9N_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9083824.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="ACHA9N_000329"
     CDS             32566..32790
                     /locus_tag="ACHA9N_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9083825.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="ACHA9N_000330"
     CDS             32932..34683
                     /locus_tag="ACHA9N_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9083826.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="ACHA9N_000331"
     CDS             34683..36467
                     /locus_tag="ACHA9N_000331"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9083827.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /gene="licT"
                     /locus_tag="ACHA9N_000332"
     CDS             36793..37644
                     /gene="licT"
                     /locus_tag="ACHA9N_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9083828.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37737..39623
                     /locus_tag="ACHA9N_000333"
                     /pseudo
     CDS             37737..39623
                     /locus_tag="ACHA9N_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(39663..40565)
                     /locus_tag="ACHA9N_000334"
     CDS             complement(39663..40565)
                     /locus_tag="ACHA9N_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9083829.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="ACHA9N_000335"
     CDS             40796..42199
                     /locus_tag="ACHA9N_000335"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9083830.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="ACHA9N_000336"
     CDS             42199..43632
                     /locus_tag="ACHA9N_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9083831.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="ACHA9N_000337"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="ACHA9N_000337"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9083832.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="ACHA9N_000338"
     CDS             44917..46644
                     /locus_tag="ACHA9N_000338"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9083833.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="ACHA9N_000339"
     CDS             46659..47009
                     /locus_tag="ACHA9N_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9083834.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="ACHA9N_000340"
     CDS             complement(47101..47961)
                     /locus_tag="ACHA9N_000340"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9083835.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="ACHA9N_000341"
     CDS             48053..48481
                     /locus_tag="ACHA9N_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9083836.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /gene="rpsU"
                     /locus_tag="ACHA9N_000342"
     CDS             48643..48819
                     /gene="rpsU"
                     /locus_tag="ACHA9N_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9083837.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="ACHA9N_000343"
     CDS             48846..49292
                     /locus_tag="ACHA9N_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9083838.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACHA9N_000344"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="ACHA9N_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9083839.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="ACHA9N_000345"
     CDS             51531..52502
                     /locus_tag="ACHA9N_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9083840.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="ACHA9N_000346"
     CDS             52526..54718
                     /locus_tag="ACHA9N_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9083841.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="ACHA9N_000347"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="ACHA9N_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9083842.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="ACHA9N_000348"
     CDS             55195..55590
                     /locus_tag="ACHA9N_000348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9083843.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="ACHA9N_000349"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="ACHA9N_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9083844.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="ACHA9N_000350"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="ACHA9N_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9083845.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="ACHA9N_000351"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="ACHA9N_000351"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9083846.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /gene="glyS"
                     /locus_tag="ACHA9N_000352"
     CDS             58762..60837
                     /gene="glyS"
                     /locus_tag="ACHA9N_000352"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9083847.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DAYYJC010000006        60541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000006 DAYYJC010000000
VERSION     DAYYJC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.006"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1535)
                     /locus_tag="ACHA9N_000353"
     CDS             complement(12..1535)
                     /locus_tag="ACHA9N_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9083848.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ACHA9N_000354"
     CDS             1787..2968
                     /locus_tag="ACHA9N_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9083849.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHA9N_000355"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ACHA9N_000355"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9083850.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ACHA9N_000356"
     CDS             complement(5053..7764)
                     /locus_tag="ACHA9N_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9083851.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ACHA9N_000357"
     CDS             complement(7774..8475)
                     /locus_tag="ACHA9N_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9083852.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ACHA9N_000358"
     CDS             8661..9260
                     /locus_tag="ACHA9N_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9083853.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ACHA9N_000359"
     CDS             9341..10354
                     /locus_tag="ACHA9N_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9083854.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHA9N_000360"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ACHA9N_000360"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9083855.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHA9N_000361"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ACHA9N_000361"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9083856.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ACHA9N_000362"
     CDS             11802..13175
                     /locus_tag="ACHA9N_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9083857.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ACHA9N_000363"
     CDS             13532..15676
                     /locus_tag="ACHA9N_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9083858.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ACHA9N_000364"
     CDS             complement(15728..16768)
                     /locus_tag="ACHA9N_000364"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9083859.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ACHA9N_000365"
     CDS             complement(17045..18847)
                     /locus_tag="ACHA9N_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9083860.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ACHA9N_000366"
     CDS             complement(18861..19613)
                     /locus_tag="ACHA9N_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9083861.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ACHA9N_000367"
     CDS             complement(19622..19984)
                     /locus_tag="ACHA9N_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9083862.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ACHA9N_000368"
     CDS             complement(20160..21032)
                     /locus_tag="ACHA9N_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9083863.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ACHA9N_000369"
     CDS             complement(21058..21570)
                     /locus_tag="ACHA9N_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9083864.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ACHA9N_000370"
     CDS             complement(21708..22499)
                     /locus_tag="ACHA9N_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9083865.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ACHA9N_000371"
     CDS             complement(22554..23162)
                     /locus_tag="ACHA9N_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9083866.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ACHA9N_000372"
     CDS             complement(23155..23472)
                     /locus_tag="ACHA9N_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9083867.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ACHA9N_000373"
     CDS             complement(23628..24356)
                     /locus_tag="ACHA9N_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9083868.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ACHA9N_000374"
     CDS             24515..25396
                     /locus_tag="ACHA9N_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9083869.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ACHA9N_000375"
     CDS             complement(25633..26676)
                     /locus_tag="ACHA9N_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9083870.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ACHA9N_000376"
     CDS             complement(26679..27347)
                     /locus_tag="ACHA9N_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9083871.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ACHA9N_000377"
     CDS             27489..29825
                     /locus_tag="ACHA9N_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9083872.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ACHA9N_000378"
     CDS             complement(29940..30251)
                     /locus_tag="ACHA9N_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083873.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ACHA9N_000379"
     CDS             30432..31907
                     /locus_tag="ACHA9N_000379"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9083874.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ACHA9N_000380"
     CDS             31954..32538
                     /locus_tag="ACHA9N_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9083875.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ACHA9N_000381"
     CDS             32540..32959
                     /locus_tag="ACHA9N_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9083876.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ACHA9N_000382"
     CDS             complement(33240..33524)
                     /locus_tag="ACHA9N_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9083877.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ACHA9N_000383"
     CDS             complement(33876..34586)
                     /locus_tag="ACHA9N_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9083878.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ACHA9N_000384"
     CDS             complement(34583..36055)
                     /locus_tag="ACHA9N_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9083879.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ACHA9N_000385"
     CDS             36193..36666
                     /locus_tag="ACHA9N_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9083880.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ACHA9N_000386"
     CDS             complement(36819..37370)
                     /locus_tag="ACHA9N_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9083881.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ACHA9N_000387"
     CDS             37475..38005
                     /locus_tag="ACHA9N_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9083882.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ACHA9N_000388"
     CDS             complement(38488..38931)
                     /locus_tag="ACHA9N_000388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083883.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ACHA9N_000389"
     CDS             complement(38947..40107)
                     /locus_tag="ACHA9N_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9083884.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ACHA9N_000390"
     CDS             40614..42314
                     /locus_tag="ACHA9N_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083885.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ACHA9N_000391"
     CDS             complement(42416..43249)
                     /locus_tag="ACHA9N_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9083886.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ACHA9N_000392"
     CDS             complement(43553..45718)
                     /locus_tag="ACHA9N_000392"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9083887.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ACHA9N_000393"
     CDS             45978..48272
                     /locus_tag="ACHA9N_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9083888.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHA9N_000394"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ACHA9N_000394"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9083889.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ACHA9N_000395"
     CDS             48923..49930
                     /locus_tag="ACHA9N_000395"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9083890.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ACHA9N_000396"
     CDS             49952..50971
                     /locus_tag="ACHA9N_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9083891.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHA9N_000397"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ACHA9N_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9083892.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHA9N_000398"
     CDS             complement(52729..54150)
                     /gene="tyrP"
                     /locus_tag="ACHA9N_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9083893.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHA9N_000399"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ACHA9N_000399"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9083894.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHA9N_000400"
     CDS             complement(56561..57817)
                     /gene="tyrS"
                     /locus_tag="ACHA9N_000400"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9083895.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ACHA9N_000401"
     CDS             58397..59698
                     /locus_tag="ACHA9N_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9083896.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ACHA9N_000402"
     CDS             complement(59767..60366)
                     /locus_tag="ACHA9N_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9083897.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18279 a        12374 c        10842 g        19046 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 a
//
LOCUS       DAYYJC010000007        50543 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000007 DAYYJC010000000
VERSION     DAYYJC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.007"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..445
                     /locus_tag="ACHA9N_000403"
     CDS             <1..445
                     /locus_tag="ACHA9N_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM9083898.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            726..1946
                     /locus_tag="ACHA9N_000404"
     CDS             726..1946
                     /locus_tag="ACHA9N_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9083899.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1943..4663
                     /locus_tag="ACHA9N_000405"
     CDS             1943..4663
                     /locus_tag="ACHA9N_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9083900.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4647..5591
                     /locus_tag="ACHA9N_000406"
     CDS             4647..5591
                     /locus_tag="ACHA9N_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9083901.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5660..6670)
                     /locus_tag="ACHA9N_000407"
     CDS             complement(5660..6670)
                     /locus_tag="ACHA9N_000407"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9083902.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6850..7194)
                     /locus_tag="ACHA9N_000408"
     CDS             complement(6850..7194)
                     /locus_tag="ACHA9N_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9083903.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7187..7621)
                     /locus_tag="ACHA9N_000409"
     CDS             complement(7187..7621)
                     /locus_tag="ACHA9N_000409"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9083904.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7759..8496
                     /locus_tag="ACHA9N_000410"
     CDS             7759..8496
                     /locus_tag="ACHA9N_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9083905.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8489..9700
                     /locus_tag="ACHA9N_000411"
     CDS             8489..9700
                     /locus_tag="ACHA9N_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9083906.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9904..10686
                     /locus_tag="ACHA9N_000412"
     CDS             9904..10686
                     /locus_tag="ACHA9N_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9083907.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10756..11418
                     /gene="trmB"
                     /locus_tag="ACHA9N_000413"
     CDS             10756..11418
                     /gene="trmB"
                     /locus_tag="ACHA9N_000413"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9083908.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11516..13270)
                     /gene="ade"
                     /locus_tag="ACHA9N_000414"
     CDS             complement(11516..13270)
                     /gene="ade"
                     /locus_tag="ACHA9N_000414"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9083909.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13402..14331
                     /gene="comGA"
                     /locus_tag="ACHA9N_000415"
     CDS             13402..14331
                     /gene="comGA"
                     /locus_tag="ACHA9N_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9083910.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14288..15337
                     /gene="comGB"
                     /locus_tag="ACHA9N_000416"
     CDS             14288..15337
                     /gene="comGB"
                     /locus_tag="ACHA9N_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9083911.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15334..15612
                     /gene="comGC"
                     /locus_tag="ACHA9N_000417"
     CDS             15334..15612
                     /gene="comGC"
                     /locus_tag="ACHA9N_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9083912.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15587..16057
                     /gene="comGD"
                     /locus_tag="ACHA9N_000418"
     CDS             15587..16057
                     /gene="comGD"
                     /locus_tag="ACHA9N_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9083913.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16029..16376
                     /locus_tag="ACHA9N_000419"
     CDS             16029..16376
                     /locus_tag="ACHA9N_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083914.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16339..16764
                     /gene="comGF"
                     /locus_tag="ACHA9N_000420"
     CDS             16339..16764
                     /gene="comGF"
                     /locus_tag="ACHA9N_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9083915.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16736..17095
                     /gene="comGG"
                     /locus_tag="ACHA9N_000421"
     CDS             16736..17095
                     /gene="comGG"
                     /locus_tag="ACHA9N_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9083916.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17267..18274
                     /locus_tag="ACHA9N_000422"
     CDS             17267..18274
                     /locus_tag="ACHA9N_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9083917.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18299..19483
                     /locus_tag="ACHA9N_000423"
     CDS             18299..19483
                     /locus_tag="ACHA9N_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9083918.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19920..21503)
                     /gene="abc-f"
                     /locus_tag="ACHA9N_000424"
     CDS             complement(19920..21503)
                     /gene="abc-f"
                     /locus_tag="ACHA9N_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9083919.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21608..21736)
                     /locus_tag="ACHA9N_000425"
     CDS             complement(21608..21736)
                     /locus_tag="ACHA9N_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083920.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22081..22224)
                     /locus_tag="ACHA9N_000426"
     CDS             complement(22081..22224)
                     /locus_tag="ACHA9N_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9083921.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22446..22925)
                     /locus_tag="ACHA9N_000427"
     CDS             complement(22446..22925)
                     /locus_tag="ACHA9N_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9083922.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22998..23282)
                     /locus_tag="ACHA9N_000428"
     CDS             complement(22998..23282)
                     /locus_tag="ACHA9N_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083923.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23360..23554)
                     /gene="ssrS"
                     /locus_tag="ACHA9N_000429"
     ncRNA           complement(23360..23554)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA9N_000429"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23626..24912)
                     /locus_tag="ACHA9N_000430"
     CDS             complement(23626..24912)
                     /locus_tag="ACHA9N_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9083924.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25050..25721
                     /locus_tag="ACHA9N_000431"
     CDS             25050..25721
                     /locus_tag="ACHA9N_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9083925.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25687..26175
                     /locus_tag="ACHA9N_000432"
     CDS             25687..26175
                     /locus_tag="ACHA9N_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9083926.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26188..27135
                     /gene="prmA"
                     /locus_tag="ACHA9N_000433"
     CDS             26188..27135
                     /gene="prmA"
                     /locus_tag="ACHA9N_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9083927.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27136..27900
                     /locus_tag="ACHA9N_000434"
     CDS             27136..27900
                     /locus_tag="ACHA9N_000434"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9083928.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28133..30346
                     /locus_tag="ACHA9N_000435"
     CDS             28133..30346
                     /locus_tag="ACHA9N_000435"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9083929.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30357..30803
                     /gene="dtd"
                     /locus_tag="ACHA9N_000436"
     CDS             30357..30803
                     /gene="dtd"
                     /locus_tag="ACHA9N_000436"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9083930.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30974..32233
                     /locus_tag="ACHA9N_000437"
     CDS             30974..32233
                     /locus_tag="ACHA9N_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9083931.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32322..33269)
                     /locus_tag="ACHA9N_000438"
     CDS             complement(32322..33269)
                     /locus_tag="ACHA9N_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9083932.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33663..34955
                     /gene="hisS"
                     /locus_tag="ACHA9N_000439"
     CDS             33663..34955
                     /gene="hisS"
                     /locus_tag="ACHA9N_000439"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9083933.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34970..36739
                     /gene="aspS"
                     /locus_tag="ACHA9N_000440"
     CDS             34970..36739
                     /gene="aspS"
                     /locus_tag="ACHA9N_000440"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9083934.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36848..37723
                     /locus_tag="ACHA9N_000441"
     CDS             36848..37723
                     /locus_tag="ACHA9N_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9083935.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37989..39872
                     /locus_tag="ACHA9N_000442"
     CDS             37989..39872
                     /locus_tag="ACHA9N_000442"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9083936.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39947..40231)
                     /locus_tag="ACHA9N_000443"
     CDS             complement(39947..40231)
                     /locus_tag="ACHA9N_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9083937.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40221..40955)
                     /locus_tag="ACHA9N_000444"
     CDS             complement(40221..40955)
                     /locus_tag="ACHA9N_000444"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9083938.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41084..41707
                     /locus_tag="ACHA9N_000445"
     CDS             41084..41707
                     /locus_tag="ACHA9N_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9083939.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41899..43020
                     /locus_tag="ACHA9N_000446"
     CDS             41899..43020
                     /locus_tag="ACHA9N_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9083940.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43027..46155
                     /locus_tag="ACHA9N_000447"
     CDS             43027..46155
                     /locus_tag="ACHA9N_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFM9083941.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46174..49368
                     /locus_tag="ACHA9N_000448"
     CDS             46174..49368
                     /locus_tag="ACHA9N_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9083942.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49514..50221)
                     /locus_tag="ACHA9N_000449"
     CDS             complement(49514..50221)
                     /locus_tag="ACHA9N_000449"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9083943.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16510 a         8868 c        10520 g        14645 t
ORIGIN      
        1 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
       61 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      121 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      181 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      241 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
      301 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
      361 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
      421 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
      481 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
      541 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
      601 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
      661 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
      721 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
      781 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
      841 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
      901 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
      961 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1021 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1081 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1141 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1201 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1261 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     1321 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     1381 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     1441 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     1501 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     1561 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     1621 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     1681 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     1741 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     1801 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     1861 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     1921 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     1981 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2041 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2101 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2161 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2221 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     2281 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     2341 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     2401 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     2461 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     2521 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     2581 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     2641 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     2701 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     2761 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     2821 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     2881 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     2941 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3001 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3061 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3121 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3181 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3241 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     3301 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     3361 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     3421 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     3481 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     3541 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     3601 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     3661 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     3721 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     3781 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     3841 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     3901 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     3961 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4021 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4081 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4141 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4201 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4261 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     4321 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     4381 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     4441 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     4501 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     4561 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     4621 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     4681 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     4741 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     4801 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     4861 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     4921 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     4981 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5041 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5101 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5161 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5221 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     5281 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     5341 tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc
     5401 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     5461 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     5521 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     5581 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     5641 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     5701 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     5761 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     5821 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     5881 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     5941 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6001 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6061 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6121 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6181 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6241 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     6301 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     6361 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     6421 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     6481 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     6541 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     6601 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     6661 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     6721 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     6781 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     6841 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     6901 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     6961 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7021 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7081 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7141 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7201 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7261 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     7321 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     7381 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     7441 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     7501 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     7561 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     7621 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     7681 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     7741 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     7801 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     7861 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     7921 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     7981 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8041 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8101 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8161 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8221 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     8281 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     8341 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     8401 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     8461 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     8521 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     8581 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     8641 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     8701 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     8761 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     8821 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     8881 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     8941 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9001 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9061 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9121 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9181 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9241 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
     9301 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
     9361 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
     9421 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
     9481 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
     9541 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
     9601 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
     9661 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
     9721 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
     9781 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
     9841 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
     9901 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
     9961 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10021 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10081 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10141 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10201 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10261 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    10321 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    10381 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    10441 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    10501 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    10561 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    10621 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    10681 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    10741 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    10801 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    10861 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    10921 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    10981 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11041 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11101 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11161 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11221 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    11281 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    11341 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    11401 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    11461 aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    11521 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    11581 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    11641 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    11701 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    11761 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    11821 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    11881 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    11941 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12001 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12061 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12121 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12181 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12241 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    12301 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    12361 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    12421 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    12481 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    12541 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    12601 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    12661 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    12721 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    12781 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    12841 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    12901 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    12961 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13021 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13081 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13141 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13201 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13261 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggctt
    13321 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    13381 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    13441 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    13501 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    13561 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    13621 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    13681 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    13741 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    13801 cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct aatgtatcgg
    13861 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    13921 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    13981 aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14041 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14101 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14161 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14221 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    14281 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    14341 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    14401 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    14461 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    14521 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    14581 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    14641 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    14701 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    14761 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    14821 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    14881 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    14941 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15001 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15061 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15121 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15181 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15241 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    15301 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    15361 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    15421 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    15481 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    15541 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    15601 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    15661 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    15721 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    15781 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    15841 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    15901 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    15961 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16021 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16081 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16141 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16201 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16261 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    16321 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    16381 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    16441 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    16501 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    16561 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    16621 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    16681 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    16741 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    16801 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    16861 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    16921 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    16981 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17041 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17101 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17161 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17221 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    17281 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    17341 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    17401 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    17461 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    17521 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    17581 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    17641 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    17701 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    17761 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    17821 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    17881 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    17941 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18001 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18061 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18121 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18181 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18241 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    18301 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    18361 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    18421 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    18481 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    18541 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    18601 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    18661 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    18721 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    18781 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    18841 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    18901 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    18961 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19021 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19081 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19141 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19201 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19261 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    19321 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    19381 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    19441 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    19501 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    19561 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    19621 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    19681 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    19741 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    19801 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    19861 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    19921 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    19981 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20041 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20101 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20161 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20221 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    20281 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    20341 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    20401 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    20461 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    20521 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    20581 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    20641 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    20701 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    20761 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    20821 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    20881 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    20941 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21001 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acagtgtgga
    21061 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21121 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21181 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21241 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    21301 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    21361 aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc aattttttca
    21421 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    21481 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    21541 tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    21601 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    21661 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    21721 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    21781 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    21841 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    21901 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    21961 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22021 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22081 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22141 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22201 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22261 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa
    22321 ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt
    22381 cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct
    22441 acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca
    22501 acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa
    22561 cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct
    22621 gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta
    22681 ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca
    22741 gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca
    22801 atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta
    22861 aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt
    22921 aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta
    22981 gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg
    23041 aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg
    23101 aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt
    23161 aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta
    23221 aataaataat agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc
    23281 atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg
    23341 ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat
    23401 cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc
    23461 atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca
    23521 cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta
    23581 aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg
    23641 atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc
    23701 tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt
    23761 gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt
    23821 cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg
    23881 aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca
    23941 aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc
    24001 agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc
    24061 ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg
    24121 ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga
    24181 aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca
    24241 ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag
    24301 tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag
    24361 gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc
    24421 ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa
    24481 gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc
    24541 tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt
    24601 atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc
    24661 cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata
    24721 attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata
    24781 tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc
    24841 gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa
    24901 tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt
    24961 agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa
    25021 tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt
    25081 tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg
    25141 acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag
    25201 aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg
    25261 aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta
    25321 ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga
    25381 ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg
    25441 gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata
    25501 gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta
    25561 gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg
    25621 gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga
    25681 aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg
    25741 acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt
    25801 cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg
    25861 caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt
    25921 tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa
    25981 tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat
    26041 ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat
    26101 caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg
    26161 tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa
    26221 agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg
    26281 attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat
    26341 aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg
    26401 actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt
    26461 ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca
    26521 acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca
    26581 agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt
    26641 atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca
    26701 gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt
    26761 gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt
    26821 cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct
    26881 aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca
    26941 gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg
    27001 atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa
    27061 gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa
    27121 ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat
    27181 tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag
    27241 gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt
    27301 actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca
    27361 agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag
    27421 aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt
    27481 aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag
    27541 gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa
    27601 gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca
    27661 aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga
    27721 atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta
    27781 gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct
    27841 ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa
    27901 cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt
    27961 ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa
    28021 agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag
    28081 atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa
    28141 agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga
    28201 acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt
    28261 tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga
    28321 tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac
    28381 agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga
    28441 cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa
    28501 ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc
    28561 tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat
    28621 cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat
    28681 caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat
    28741 cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga
    28801 agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa
    28861 acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta
    28921 tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg
    28981 agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc
    29041 aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt
    29101 agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca
    29161 ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt
    29221 aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat
    29281 ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga
    29341 cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga
    29401 tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa
    29461 attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg
    29521 cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa
    29581 agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac
    29641 agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga
    29701 tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag
    29761 ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca
    29821 gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa
    29881 agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag
    29941 tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg
    30001 gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga
    30061 acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga
    30121 tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa
    30181 tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac
    30241 gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat
    30301 caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga
    30361 gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa
    30421 aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg
    30481 tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa
    30541 tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt
    30601 ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag
    30661 ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta
    30721 ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca
    30781 ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac
    30841 gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc
    30901 ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag
    30961 gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa
    31021 attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt
    31081 cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa
    31141 agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa
    31201 gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa
    31261 aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga
    31321 taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag
    31381 taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc
    31441 taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac
    31501 ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca
    31561 aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata
    31621 tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt
    31681 tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg
    31741 acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt
    31801 acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc
    31861 gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat
    31921 tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat
    31981 gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat
    32041 tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct
    32101 tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga
    32161 acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact
    32221 ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc
    32281 ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct
    32341 tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca
    32401 gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg
    32461 taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt
    32521 acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt
    32581 cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa
    32641 ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat
    32701 aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat
    32761 ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg
    32821 atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt
    32881 tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca
    32941 tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt
    33001 acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt
    33061 acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa
    33121 ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg
    33181 tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact
    33241 tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa
    33301 caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt
    33361 gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag
    33421 taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat
    33481 tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt
    33541 cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc
    33601 cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa
    33661 ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat
    33721 ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc
    33781 gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata
    33841 tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac
    33901 cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt
    33961 atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa
    34021 aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg
    34081 caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc
    34141 aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag
    34201 cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc
    34261 ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg
    34321 cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg
    34381 ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg
    34441 gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg
    34501 cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc
    34561 cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg
    34621 cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag
    34681 acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag
    34741 ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga
    34801 atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca
    34861 aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg
    34921 atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag
    34981 aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg
    35041 atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga
    35101 aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga
    35161 taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa
    35221 tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat
    35281 tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga
    35341 aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa
    35401 attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga
    35461 tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc
    35521 ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca
    35581 attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga
    35641 agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt
    35701 aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt
    35761 tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg
    35821 ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac
    35881 agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa
    35941 agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg
    36001 tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg
    36061 attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac
    36121 agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc
    36181 tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa
    36241 attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg
    36301 atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc
    36361 tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt
    36421 aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct
    36481 aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta
    36541 cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc
    36601 cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt
    36661 gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt
    36721 aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt
    36781 tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg
    36841 cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc
    36901 ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg
    36961 acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc
    37021 actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt
    37081 tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac
    37141 tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat
    37201 ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg
    37261 cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc
    37321 gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt
    37381 tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga
    37441 tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa
    37501 agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga
    37561 aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact
    37621 gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga
    37681 aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa
    37741 ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta
    37801 gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat
    37861 gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct
    37921 acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg
    37981 aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg
    38041 ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga
    38101 aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc
    38161 tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa
    38221 gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca
    38281 ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt
    38341 atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg
    38401 agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc
    38461 cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg
    38521 tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg
    38581 atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg
    38641 cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa
    38701 atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt
    38761 tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg
    38821 tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc
    38881 cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta
    38941 tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag
    39001 tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc
    39061 tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga
    39121 tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg
    39181 ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa
    39241 aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta
    39301 gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg
    39361 cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc
    39421 tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca
    39481 gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg
    39541 gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg
    39601 gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg
    39661 tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt
    39721 acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg
    39781 aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta
    39841 cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca
    39901 tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag
    39961 gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt
    40021 tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca
    40081 tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt
    40141 cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga
    40201 ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat
    40261 tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct
    40321 tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat
    40381 tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga
    40441 tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg
    40501 atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg
    40561 ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa
    40621 gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt
    40681 tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt
    40741 tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt
    40801 cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc
    40861 acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg
    40921 tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat
    40981 agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat
    41041 tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat
    41101 gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa
    41161 agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga
    41221 cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga
    41281 actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa
    41341 aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa
    41401 tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat
    41461 ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt
    41521 gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga
    41581 cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca
    41641 agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa
    41701 aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc
    41761 agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat
    41821 atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac
    41881 attttttgag gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa
    41941 ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc
    42001 gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc
    42061 ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa
    42121 aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt
    42181 acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa
    42241 ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg
    42301 agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata
    42361 gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt
    42421 gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg
    42481 atccctggtg aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa
    42541 gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct
    42601 gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat
    42661 tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg
    42721 ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact
    42781 gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg
    42841 gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa
    42901 atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta
    42961 actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga
    43021 tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc
    43081 gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct
    43141 ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga
    43201 cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg
    43261 actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt
    43321 ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt
    43381 tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg
    43441 atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa
    43501 ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat
    43561 ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa
    43621 cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt
    43681 ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac
    43741 catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag
    43801 gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat
    43861 gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata
    43921 gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc
    43981 cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa
    44041 acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac
    44101 cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg
    44161 atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt
    44221 tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag
    44281 gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt
    44341 cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa
    44401 gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa
    44461 ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc
    44521 caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt
    44581 cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag
    44641 gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact
    44701 cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat
    44761 ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa
    44821 caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta
    44881 gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag aatagagctg
    44941 gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag
    45001 cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa
    45061 caattgcaag atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag
    45121 ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa
    45181 gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat
    45241 gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt
    45301 tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa
    45361 cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta
    45421 gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca acagttagaa
    45481 gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa
    45541 attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa
    45601 ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag
    45661 aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca
    45721 aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt
    45781 ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg
    45841 tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca
    45901 ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt
    45961 attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta
    46021 gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa
    46081 gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc
    46141 tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc
    46201 attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa
    46261 gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat
    46321 ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt
    46381 aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa
    46441 aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa
    46501 ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa
    46561 taccaagtag aaacgattga gactaatcag tatcacccag agctttctat tttggcactt
    46621 tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat
    46681 gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt
    46741 cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga
    46801 agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat
    46861 aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag
    46921 actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca attttctgat
    46981 caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca
    47041 ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt
    47101 tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga
    47161 ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt
    47221 atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta
    47281 gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct
    47341 cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg
    47401 acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta
    47461 ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg
    47521 gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc
    47581 agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc
    47641 gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag
    47701 caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa
    47761 aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa
    47821 caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga
    47881 ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg
    47941 atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat
    48001 gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat
    48061 cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc
    48121 tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg
    48181 agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa
    48241 gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga
    48301 gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat
    48361 tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca
    48421 ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat
    48481 ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca
    48541 aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg
    48601 cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct
    48661 gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa
    48721 atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg
    48781 acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt
    48841 tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga
    48901 gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag
    48961 ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg
    49021 gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga
    49081 actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct
    49141 gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa
    49201 gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa
    49261 aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact
    49321 gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa
    49381 agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa
    49441 aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt
    49501 ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag
    49561 aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa
    49621 gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt
    49681 ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt
    49741 ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt
    49801 tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc
    49861 ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc
    49921 agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa
    49981 tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta
    50041 gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga
    50101 tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc
    50161 gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca
    50221 aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca
    50281 gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct
    50341 tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat
    50401 aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc
    50461 tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac tattcaaaca
    50521 taaaataacg ttatttgatt tat
//
LOCUS       DAYYJC010000008        49763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000008 DAYYJC010000000
VERSION     DAYYJC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.008"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1479
                     /locus_tag="ACHA9N_000450"
     CDS             127..1479
                     /locus_tag="ACHA9N_000450"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9083944.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            1650..1937
                     /locus_tag="ACHA9N_000451"
     CDS             1650..1937
                     /locus_tag="ACHA9N_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9083945.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            2163..2606
                     /locus_tag="ACHA9N_000452"
     CDS             2163..2606
                     /locus_tag="ACHA9N_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9083946.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(2612..3796)
                     /locus_tag="ACHA9N_000453"
     CDS             complement(2612..3796)
                     /locus_tag="ACHA9N_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9083947.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            4064..4762
                     /locus_tag="ACHA9N_000454"
     CDS             4064..4762
                     /locus_tag="ACHA9N_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9083948.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            4769..5446
                     /gene="nth"
                     /locus_tag="ACHA9N_000455"
     CDS             4769..5446
                     /gene="nth"
                     /locus_tag="ACHA9N_000455"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFM9083949.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(5572..6105)
                     /locus_tag="ACHA9N_000456"
     CDS             complement(5572..6105)
                     /locus_tag="ACHA9N_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083950.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(6402..8783)
                     /locus_tag="ACHA9N_000457"
     CDS             complement(6402..8783)
                     /locus_tag="ACHA9N_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9083951.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(8773..9420)
                     /gene="recU"
                     /locus_tag="ACHA9N_000458"
     CDS             complement(8773..9420)
                     /gene="recU"
                     /locus_tag="ACHA9N_000458"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9083952.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            9484..10023
                     /locus_tag="ACHA9N_000459"
     CDS             9484..10023
                     /locus_tag="ACHA9N_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9083953.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            10113..10532
                     /gene="gpsB"
                     /locus_tag="ACHA9N_000460"
     CDS             10113..10532
                     /gene="gpsB"
                     /locus_tag="ACHA9N_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9083954.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            10554..10921
                     /gene="rnpB"
                     /locus_tag="ACHA9N_000461"
     ncRNA           10554..10921
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA9N_000461"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            11130..12296
                     /locus_tag="ACHA9N_000462"
     CDS             11130..12296
                     /locus_tag="ACHA9N_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9083955.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            12342..13838
                     /locus_tag="ACHA9N_000463"
     CDS             12342..13838
                     /locus_tag="ACHA9N_000463"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9083956.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            13919..14602
                     /locus_tag="ACHA9N_000464"
     CDS             13919..14602
                     /locus_tag="ACHA9N_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9083957.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            15247..16437
                     /locus_tag="ACHA9N_000465"
     CDS             15247..16437
                     /locus_tag="ACHA9N_000465"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9083958.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            16539..17492
                     /locus_tag="ACHA9N_000466"
     CDS             16539..17492
                     /locus_tag="ACHA9N_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9083959.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            17561..18910
                     /locus_tag="ACHA9N_000467"
     CDS             17561..18910
                     /locus_tag="ACHA9N_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9083960.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(19093..19416)
                     /locus_tag="ACHA9N_000468"
     CDS             complement(19093..19416)
                     /locus_tag="ACHA9N_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083961.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            19545..20708
                     /locus_tag="ACHA9N_000469"
     CDS             19545..20708
                     /locus_tag="ACHA9N_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9083962.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            20778..22127
                     /locus_tag="ACHA9N_000470"
     CDS             20778..22127
                     /locus_tag="ACHA9N_000470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9083963.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(22172..22603)
                     /locus_tag="ACHA9N_000471"
     CDS             complement(22172..22603)
                     /locus_tag="ACHA9N_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083964.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            22991..25633
                     /gene="alaS"
                     /locus_tag="ACHA9N_000472"
     CDS             22991..25633
                     /gene="alaS"
                     /locus_tag="ACHA9N_000472"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9083965.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            25833..26429
                     /locus_tag="ACHA9N_000473"
     CDS             25833..26429
                     /locus_tag="ACHA9N_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9083966.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            26551..27699
                     /locus_tag="ACHA9N_000474"
     CDS             26551..27699
                     /locus_tag="ACHA9N_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9083967.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(27737..28150)
                     /locus_tag="ACHA9N_000475"
     CDS             complement(27737..28150)
                     /locus_tag="ACHA9N_000475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9083968.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            28229..28660
                     /locus_tag="ACHA9N_000476"
     CDS             28229..28660
                     /locus_tag="ACHA9N_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9083969.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(28759..28992)
                     /locus_tag="ACHA9N_000477"
     CDS             complement(28759..28992)
                     /locus_tag="ACHA9N_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9083970.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            29175..30845
                     /locus_tag="ACHA9N_000478"
     CDS             29175..30845
                     /locus_tag="ACHA9N_000478"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9083971.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            30992..31378
                     /locus_tag="ACHA9N_000479"
     CDS             30992..31378
                     /locus_tag="ACHA9N_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083972.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            31365..32063
                     /locus_tag="ACHA9N_000480"
     CDS             31365..32063
                     /locus_tag="ACHA9N_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9083973.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            32068..32532
                     /gene="lspA"
                     /locus_tag="ACHA9N_000481"
     CDS             32068..32532
                     /gene="lspA"
                     /locus_tag="ACHA9N_000481"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9083974.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            32529..33440
                     /locus_tag="ACHA9N_000482"
     CDS             32529..33440
                     /locus_tag="ACHA9N_000482"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9083975.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            33675..34214
                     /gene="pyrR"
                     /locus_tag="ACHA9N_000483"
     CDS             33675..34214
                     /gene="pyrR"
                     /locus_tag="ACHA9N_000483"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9083976.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            34228..35511
                     /locus_tag="ACHA9N_000484"
     CDS             34228..35511
                     /locus_tag="ACHA9N_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9083977.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            35524..36450
                     /locus_tag="ACHA9N_000485"
     CDS             35524..36450
                     /locus_tag="ACHA9N_000485"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9083978.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            36469..37749
                     /locus_tag="ACHA9N_000486"
     CDS             36469..37749
                     /locus_tag="ACHA9N_000486"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9083979.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            37754..38830
                     /locus_tag="ACHA9N_000487"
     CDS             37754..38830
                     /locus_tag="ACHA9N_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9083980.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            38835..42020
                     /gene="carB"
                     /locus_tag="ACHA9N_000488"
     CDS             38835..42020
                     /gene="carB"
                     /locus_tag="ACHA9N_000488"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9083981.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            42074..42850
                     /locus_tag="ACHA9N_000489"
     CDS             42074..42850
                     /locus_tag="ACHA9N_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9083982.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            42847..43779
                     /locus_tag="ACHA9N_000490"
     CDS             42847..43779
                     /locus_tag="ACHA9N_000490"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9083983.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            43776..44492
                     /gene="pyrF"
                     /locus_tag="ACHA9N_000491"
     CDS             43776..44492
                     /gene="pyrF"
                     /locus_tag="ACHA9N_000491"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9083984.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            44499..45125
                     /gene="pyrE"
                     /locus_tag="ACHA9N_000492"
                     /pseudo
     CDS             44499..45125
                     /gene="pyrE"
                     /locus_tag="ACHA9N_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            45152..45430
                     /locus_tag="ACHA9N_000493"
     CDS             45152..45430
                     /locus_tag="ACHA9N_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083985.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            45468..46109
                     /locus_tag="ACHA9N_000494"
     CDS             45468..46109
                     /locus_tag="ACHA9N_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9083986.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            46152..46844
                     /locus_tag="ACHA9N_000495"
     CDS             46152..46844
                     /locus_tag="ACHA9N_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9083987.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            46841..47743
                     /locus_tag="ACHA9N_000496"
     CDS             46841..47743
                     /locus_tag="ACHA9N_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9083988.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(47901..49607)
                     /gene="efbA"
                     /locus_tag="ACHA9N_000497"
     CDS             complement(47901..49607)
                     /gene="efbA"
                     /locus_tag="ACHA9N_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9083989.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16254 a         8706 c        10685 g        14118 t
ORIGIN      
        1 tgcttgaaaa ggaatgaaac tacaaacaaa cctttgtgaa ttcacaagca ttaaactgtt
       61 gtatccgttt tatttctgtt atagtagttt tgtcgaagga caaaatgtta atcggaggga
      121 ccataaatga aagtagtagt aattggctgt actcatgcag gtactgcagc tgtaaaaagt
      181 attttaaaaa atcatccaga agcagaagtg acagtatatg aaagaaatga caatatttct
      241 ttcttatctt gcggtatcgc actttatgtc ggcggagtag taaaggaccc agcaggcttg
      301 ttctactcaa atcctgaaga attatcttca ttaggagcaa cagttaaaat ggaacacgat
      361 gtaacagacg tcgatacaga aaataaaaca gtaacggcaa aagatctgaa aacaggagaa
      421 acagaaacgg tttcttatga taaattagtg atgacaactg gttcatggcc tattatccca
      481 ccgattagtg gaatcgatgc gaaaaatgta ctcctatgca aaaactacaa tcaagcaaat
      541 gaaattattg cacaagcgaa agaagcaaaa cgtgtagtaa ttgttggtgg tggatatatc
      601 ggtattgaat tagtagaagc ttttgtcgaa tctggaaaac aagtgacatt gattgatgga
      661 ttagatcgca tcttgaataa atatctagat aaaccattca cagatatctt agaaaaagaa
      721 ttgacagacc gcggtgttac tcttgcgcta ggcgaaaatg ttactgaatt tatcacagat
      781 gaagaaggaa aagtgaaaca agtagctaca ccaacagata catttgatac agatatggtc
      841 attttatgtg tgggcttccg tccaaataca aaactgatcg aaggaaaagt agatacactg
      901 ccaaacgggg caatcaaagt gaatgaatac atgcagacaa gtaatccaga catttttgca
      961 gctggagact ctgcagtagt caactacaat ccaagtggca cgcaaaacta tattcctttg
     1021 gcaacaaatg ccgttcgtca aggtttgctt gtgggaaata acttgacaga tcataaaatg
     1081 gcgtatcgtg gtactcaagg aacttcagga ctttacctat ttggctggac gatcggttcg
     1141 acaggtgtga ccaaagaaag cgcaactttg aacgatctag aagtccaagc gactgtattt
     1201 gaagataatt atcgtccaga gttcatgcca actactgaaa aagtaatgat ggagctagtc
     1261 tatgaaaaag gaacgaatcg tatcgtaggt gctcagttca tgtccaaata cgacattaca
     1321 caatcagcaa ataccatgtc tttagctgtt caaaataaaa tgactgtaga agatctcgca
     1381 ctttcagatt tcttcttcca accacatttt gaccgcccat ggaattactt gaacctactt
     1441 gcacaagcag cgctaggcga aatcgaaact gcacaataaa gaaaaagggt tgtgacacaa
     1501 ttttgtcacg accctctgtc tttttttgtg aaaagtacaa ttagttgtct ttcttcgaat
     1561 tttttgtcac aataagcagc aaagataaag ctgttacata aaagaacaag cagctttatt
     1621 tgattttgaa aagaaaggtg agaaagaaga tgaataaaga atattttgtc caacaagatg
     1681 catttttaga agattataaa agtgcttcga agaaattaac gatcaatatt gatgagttgg
     1741 cagatcaagt actctctcag ttgaatgaaa cgcaagagga atattacacg atgccagcaa
     1801 atgacgccaa agataatgtt tctcatcgtt ttcctttcag tagacgcatc tactcagatg
     1861 aaaatgacgg cacattgaat acagagtaca catatgaagg tcagccatat gtgctagaaa
     1921 ctgatgatga tctttaaaga cagtttttga aaaggccgct catccttaag tgatgacaaa
     1981 aaatcatcac aattaccgat gactgtctct ttactatctt ttaatcagaa aaaggggtta
     2041 tgacaagctt tgtcaacccc tttttttctt ggtttcagat tgggttttcc tacaaaatat
     2101 ttttatcttt gttgaatagg aaacaaaaaa acaatataat gaaagaaaaa gaaggtgtag
     2161 aaatggataa aagacaactg ctaatagaag aatatctgga tttagctaat gagattattc
     2221 atcgtaataa accagaaatg gaaaaagaat ttgcaggatt tactctaaat gaactagaag
     2281 tgattgaaca tatcgggaaa atccctgatc ctaacgtgac aaaattagcg aatgctagtt
     2341 acatgactag aggagccatc agtaagctaa caaaaaaact gatagctaga gggatcataa
     2401 acacttatca aagcgaagag aataaaaaag aaatctattt tcaattgacg ccaatcggac
     2461 aaaaaataag gaatagacac gagcaactcc atcaaaaatg ggtacagcga gatgctactg
     2521 tatttgaaaa tatgtcaaaa gaagaatttg acacagtatt tcgttttatc ggtcgataca
     2581 gagaatttat acgaagtagc aaatgaagct actatttttt attgtagtga taataggcaa
     2641 aaatcataag caaaaatccg aataccgtcg gaagaaatgg tgctgcagca taaagctgtc
     2701 ctgcgattag cgggccgatg acgcgcgata gagattgtat actttgggta ccgcccatta
     2761 caagaccttg ctggttatct gagacagaat tggatagttg gccattgaat acaggagtaa
     2821 aaatagaatc accgaaacca taacagataa tcccaaataa aaacaaaatc ggcagtagaa
     2881 gaattccaga taatgtgatg aatagatagg cgaggatttc actgaacatc cctattcttg
     2941 tgatctgctg ttctgaaaat tttttgagca atcgaggcat aatgaacaat tgcgagacag
     3001 tatccaagat tccaatcaaa gagaagctta aaccaaccaa cccagccttc cactgaaagg
     3061 cgtcaatcga aaattgagaa aagatagatt gaagagagcc agctgccaac caaacagcaa
     3121 aacctgctgt caataaagga ataacggatg aaatcttgaa taattgttgt aattggtgaa
     3181 aaggtcttac atgggagaaa cttaaatcta ctgaacgttt ttttattgga aggctttcta
     3241 gcataaatgt gtagccataa acggcattta agaaagtaaa gagtgctcca ataaatatag
     3301 gaacagaatt ccccaactca gctaatagac cccctataat aggacccagc gttgttccta
     3361 taccgactaa agcacccatc catccaaaaa ctttggttct ttcattaggt tccgtgatgt
     3421 cggcaaagta agcatataag gtggagattt ctcctgccgt caatccttca atgatacgac
     3481 caaggaacaa catccatatc gaatttccta aaccaaaaag caaatatccg atacttgatc
     3541 ctaataaact gataataagg atcggatgac gcccaaaatg atcacttaag ctaccgagaa
     3601 tgggggcaga taagaaggca gctagagcat aaacagacat gagcaaagtg atatagaaag
     3661 cttgctgatg tatggagtga gaataaggca gcgttaaaaa cggaagtaca ggactgacga
     3721 tagtaaatcc taatccagtg aataagacag agacgtatcc aaaaacttta gaatgtttat
     3781 ttgtgaaact gtacatgtaa aaactccttt atgcaataat gttaacttgg aaacaaaaat
     3841 agtgtatagt aattttgttt ccttgtcaac attctaagga acagcgaaat ctacttaatt
     3901 tatttattcc gaaaagtgaa gatacgcatc aaaaacatga acaaacaaaa atcaagtaga
     3961 cgacattgta aatctgaccg atcagtgaag taaatagaaa agaaacctgt tcttgcttcc
     4021 catgtttttt ttacgtatac tgtgattgag cggaggagaa atcatgttga atttagaaaa
     4081 ttacttaagc gctggacaaa cagtcatatc taatctttta cttaaaaatt atcataggat
     4141 cggtctacaa tctgaagaat ttttatttgt tctacagttg catatggcac agcttgaagg
     4201 agatactttc cctgatttgc agatgattgc tatggatatg ggaatcaaac aagatcagat
     4261 ttttcaaata ctggatcgtc tcgtaacaaa tggatttatc aagatagaaa cgacgattgt
     4321 caacggtaaa aaagcagatc gatataatct ttaccccatc tatgatctat taggtgagta
     4381 tttaaaaaca caagaaaaaa aatacgaaga aaaaacacaa gaaaaagaaa tccagtcagt
     4441 gtatcaatta tttgaacaag aatttggacg cccgctttct tcgatcgagt tccagcggat
     4501 cggacaatgg ctagaagaag accattatca gccggaaatt ttgaagctag cactgcggga
     4561 agccgtatta aatcaggctt atagttttaa ttacgttgac cgcattttgc tatcgtggga
     4621 aagaaaaaat ctacgtacaa aacaacaagt ggaagaagac caaaaacgaa gaaaacagca
     4681 gcttcttcaa agagaagtag aacagtcagc tcaacaagaa gaattgccga aagtctcact
     4741 gaaaaattgg ttggaggagt agcggaatat gttaagcaaa caaaaaacaa tggaagctct
     4801 agaaacgatg tatggaatgt ttcccgaagc acatggagaa ctcaagcaca ataatccatt
     4861 tgagttattg attgcagtga tcttaagtgc ccaggcgaca gatgtatcag tgaataaagc
     4921 aacgccagat ttgtttgcct cttttccgac acccgatgcg ttagcagaag cttcaatcga
     4981 tgaaatcatt cttaaaataa aaacgattgg tttgtatcga aataaagcaa aaaacatcaa
     5041 agcgtgtgca cagcagctga ttgaacgttt cgatgggcaa gtgccaacgt caagagaaga
     5101 attgatgtct cttcctggtg taggcagaaa gacagcaaat gtcgtattgg gggatgcatt
     5161 cgggatacca gcaatcgcag tagataccca cgtggaacgt gtctcaaaac gcctgaggat
     5221 ctgcaaacta gatgctacgg tcatggaagt agaagaaaca ctgatgcgga aagtaccaca
     5281 agaattatgg gtaaagacac atcatacact gatttttttt ggtagatatc attgcacggc
     5341 aagaaatccc aagtgcgagg tatgtccttt gctatcaata tgtcaagatg ggaaaaatcg
     5401 gatgcgtcta aaagaaaagg cgctaaaaaa gaaaccacgc ctgtagacgt ggtttcttta
     5461 attgcaaata aatgtatgaa ttatcttgtt attatggaga aaagctgttt cgtttatctt
     5521 ttttgcatga aaaagtaaat gtacatactc agtagctagg atggtaactt attacaattt
     5581 attgcgataa aaataaaagt tgttattttt attgggaaaa tacccgatgt agaaaataat
     5641 gccttccaat atacctataa aataagtcca gtattccaaa ggattatata aataagtgac
     5701 gtatctatct aaaggtgtaa taaaaatgat aagtgataat aaaaaaatac catttataac
     5761 taaacataaa ctaatggatt tgggcggttt caggctcatc agaaaaatat agattctggt
     5821 gaacaacaac cataaaaaag tgagaatcat tgcatgagag cttctttcca taaattttcc
     5881 tttaagagta gtgcttaaaa gtacagaaaa aataataaat gcaaggatag gagcaattaa
     5941 agtaaattta tttaataata aacaaaattt ttcagctttt ttattcttgg aataacgagg
     6001 tattgttttc gataaaaaaa gatggtgcag acaaattatg ataattaagc atactatcag
     6061 ataactcaaa atgaagcctt ctttcaaaaa attattcaaa accatatatt gtatttttta
     6121 tttgataata acaatgggat tattaagaag tggcactaaa taaaagacta gtgtgtattt
     6181 agccgattta attataaaca agtaaatgga taaagtatat gtaatgataa aagaaaaatg
     6241 gattttctaa tttttaagct aaatgttaga tcacacatgt atatgctgga tagtcttata
     6301 aaaaatcatg taatacagct tggtacatga tttttatttg tagctgtttt ttacatagac
     6361 acaaaaaaga aaccacgctt gtagacgtgg tttctttttt actatctgtt tccagaagct
     6421 gaggtgctgc tggatggtgt atggtcattt gcagcatccg ctccgctagt tgctggctca
     6481 ctggaagaag caggaggagc agatgaagac ggtgtcgagc tttcttcgga tgaagaagaa
     6541 ggaaccgttg ttgaagacgc tggctcgctt gtttctgctg tcgtgctttc ttttgaggac
     6601 tcagcagttg attcgctttg actagtagag ctagagcttt gagtcgttgt ctctgttgtt
     6661 gagcttccag gagtctggac atagctggaa gatggaatag ttgttgatgg tgtaattgca
     6721 ttcgatctag ctgtatattg atctttgtag tacagttcac cgcccacacg aattaatccg
     6781 cttggcatct cccagtctgt atttgtcacg ttagctgaaa catactgcat aagctcgcga
     6841 taaacatcag aggctacgtg tgacgattct gaagtgacag gtgtcatttt tttattgtaa
     6901 cctgtccaca cggaaatcga ataatttggc gtatagcccg caaataggat atctggatac
     6961 accccagaag aaataccaag ttttgcatac tcgtcatctg tgtaattgga tgttccggtc
     7021 ttaccggctt gatataaccc agcgatttgc gcattcgtac cagttccttc agtgatcgta
     7081 tcttttaaaa tgtctgtgat catataagct gtttctggag acatggctgt ttttccatct
     7141 ggctcataag tttcttctgt tccgtcttgg aagacgattt tattaacata ttgaggtttg
     7201 taataagtcc ctccattggc gaaagccgca taggctgctg ccatttttaa ggaagaagct
     7261 ccgtattttg tcccatcttg ttcttccgta ttactagaga tcgcattcga ttgatggatc
     7321 gtactgtatt cgatcccaag attttttaag aaagaagcca ctttatctga accgacttca
     7381 ttgaataatt tgactgctgg aacattacgg gataagtaaa gtgcttggcg taaagtaatg
     7441 gtgcccatat attgattatc ccagtttcct acaggagtcg aagtcccttc atagttatac
     7501 ggtgcgtcag taatagtttt tccagtagaa tatttcaaat attcaaaagc tggtccgtaa
     7561 tctgtgacag gtttcatcgt agaaccaaaa tcacgggaag tattcactgc taagttgttg
     7621 cctaaagtta cgtcttctgc gatgtgacgt ccgccaatct gggcttttac tttcccggta
     7681 ttggtgtcga taagggtgga ggcaacttgc atctcgtcgt ctgggtaaga gacatactga
     7741 tccgtgttga cgatatcata tagtttcttt tgggcgtcca gatccaaatt cgtataaatc
     7801 tctaaaccgt ctgtgtagac attcttatct gttttttctt gtacttcatt gatgacttct
     7861 ttgacataat tatcgacgat cttagtattg tcatcggatt gagttagttc ttgcagacca
     7921 tccgttaccg gtacattaac agcttgatca tattctgttt gagagatttt ttcgttttga
     7981 agcatcgtat acaaaacagt atctcgtctt ttcttcgcct gatctggata aacataagga
     8041 tcataggcgg aaggggcttg aggcatccca gcaagcaatg ctgtttgcgg taaagaaagt
     8101 tctgataatt tcttaccaaa gtacatctca gaagctgtct ccatcccgta caagccgttt
     8161 gacatataca ctttatttac gtagtaggtc aagatctctt gtttcgattt tttttgttcc
     8221 aatcttacag ccatccacgc ttcttgggcc ttgcgtttca gagtctgatc ctcggcactg
     8281 gtggagaaga aggaaagttt gatcaattgc tgtgtcaaag tacttccgcc ttgcagtccg
     8341 ccagaggtaa agttagagag ggcagaaccg atgatccgaa tagggtcaac cccgatgtgt
     8401 ttataaaaac ggcgatcttc taccgacacg atcgcatctt ctaatgtttt cggtaattca
     8461 ttcgcagaaa ttttttcacg cttttctgcg cctaaatctt cgaatagttc gccatcttgt
     8521 gtatatagtt tggaagatac ggtagcgtct aattttttat ccgtcaattc gggcgcatct
     8581 tttgcgtaat accagaaaag tcctacacct gctaaaaaga ggatacagaa aaatgagagt
     8641 attcctaaaa ggatcttgat aaataatcct tttttatgtg ttccagagga ctttccagaa
     8701 cctttgccag aattcttctt cacactcttt ttaggagtag gctgtttctg tcgtctagag
     8761 cttctagttt gttcatttgc cattcttgct ttctccttca atagataatg ttgataaata
     8821 gaagtcaacc gcgtccaaat aaggaatccg cggagaaata ccagttttga tctctattcc
     8881 tagttcttcg atcaaagata aaggtagtga tttgcgtcct ttttcctttt gggcgtccca
     8941 gtaagcaatc aaatgaacac tattcaaata aaaacagcgc ttcaaagacg aaaaccataa
     9001 caagacaaag cagaccccat tttgttcgag gcatgctttc atatgttcga tttgatgggc
     9061 atggaagttt ttaaagggaa aagatgtttt gttcttggtt tcttttgctt caaagtccaa
     9121 gtaaaagcct cgatagacgc cattatagtc tgtagttgag gcttgggtaa agtaggcttc
     9181 cttgatcaca gcagcacttc tcttaggata gtctactttc acgatttgga taggggtagg
     9241 ctttttatgg ataaccgcca acccgcggga aagataatat ttattacttt cattgattgc
     9301 ttcttcaaag cgcatccccc gattgccgaa ttcagtgatc tgatttcttt tttcttgaat
     9361 tttttcgacg ggagaggcat tcttataata aggttggcca tttggatagc gcaataccat
     9421 ctttaatcac actcctaggc cacattatag caaaaaagcg gatagaaaga aaaggaatct
     9481 gagatggata agtcaaaagt aatctatgtg tctgggtatc gaagtttcga actcggtgtt
     9541 tttaaagaaa atgatcctaa aatccaagtt gttaaaaatg ttttaaaaaa agaactccat
     9601 cagttagcag aagaagggct ggaatgggta cttgtcagtg ggaatttggg cgtcgaacta
     9661 tgggctgtgc aaatagtagc ggaattgaaa gaagaatatc cggaactatc tcttggtatt
     9721 ttgtatcctt tttctgattt tggtaataac tggaatgaag ggaaccaaga aaaaaaacaa
     9781 ttagcagaac aattagcaga ttatgtcgaa gctgttagcc atcaacccta ccaatcacct
     9841 agtcagctaa agaatcacac tcgttttata cttgagcata cagtgggctg tctgctgatc
     9901 tatgatgaag aattccctgg aaaaacgaag tatttttttg aagatgcaca gaattaccaa
     9961 gcagatcatc cctacgaaat tcggctgatc agtatggacg atcttcaaaa ttttagtgaa
    10021 taatcgatat tgtgtttgat ttatcaactt ttttcctata caatgaaaaa ggtagaaatg
    10081 ttattacgta aatgaaaact agaggtgtaa atatggcgaa tttggtttat agtcctaaag
    10141 acattttaca acaagaattc aaaacaaaaa tgcgcggcta tgatcccgtt gaagttgatg
    10201 aattcttaga caatatcatc aaagattatg aagcttacag caaagaactc cttgctttac
    10261 aagaagaaaa cgatcgattg agtgcaaaag tagctcaatt atctaaaact caaggagcag
    10321 ctcaaacacg cgtgcaacaa acagaggcac caaagagcgc agcagtgacc aattttgata
    10381 tcttaaaacg cttgtcaaat cttgaacgtg aagtatttgg taaaaagctt gatcaacaag
    10441 caagtgcagt caaaccagca caacctaatc caaataacta cacaaatgca gacacaagct
    10501 tagatgataa tgaaaaaaca cgtcaatttt aatttttgat taatgaattt gcggttttcg
    10561 ggtaatcgcg gtctgacttg atcaggctga ggaaagtcca tgctcgcaca ggctgtgatg
    10621 cctgtagtgt tcgtgcttag cgaaaccata agctaaggta ctctttggag taacggcagg
    10681 aaaacaagct aaggcttcgg ctatgcttaa gtatccttga aagtgccaca gtgacgaagc
    10741 ggctggggaa acccatccgg tggaacgcgg taaacccctc gagcgagcaa cccaaacaat
    10801 ggtaggggcg ctttttgtct ggaagaagaa caggacaaaa aggcagtata actgcagata
    10861 gatggttacc gctttgtttt ttctccctgg aaaagcaaag tacagaacat ggcttataga
    10921 aaaccgcaaa catcagggaa ttaggtcgtg acaaaagtaa tgcgttataa gaaacaagaa
    10981 aaacatttga aagctatttt tcaatacttc agatgaatcg ttcttattac aaaacaaact
    11041 tttgtatcac cggttattcg gaatttgagt aaaagtgtgg ggtaggactg atgtcccacc
    11101 tccttttttt ataaataagg agattgtgta tggaaaaaaa caggaagttc aacctcgtcg
    11161 caactgcggc aagcgggcta gaagcactgg ttggtaaaga attaagagat cttggaatcg
    11221 aatgccaagt tgagaacgga cgtgcacgtt ttgaaggaac aatggaaacg atcgctacag
    11281 ctaatttatg gttacgtaca gcagatcgag taaaaatcgt ggttggggaa ttcgatgcgt
    11341 atacgtttga tgaattgttt gaaaacgtaa aagcattgcc ttgggaagat tttcttccac
    11401 tagatgcagc ttttcctgta gctggaaaat cgatcaagtc gaaactatat agcacacctg
    11461 attgccaagc gatcacaaaa aaagcaatcg ttgaacgtct ccgtacctat tatcaccgtc
    11521 cagcttctgt accactgacg gaaactggcg cacattttca gttggaagta gctttgctga
    11581 aagaccatgt gatggtaact ctggatacga caggaccaag tttattcaaa cgtggttatc
    11641 gcttagaaaa aggtggagca ccattgaaag aaaacatggc agcagcactt attatgttga
    11701 caaattggcg aaaagaccgt ccattttatg atcctgtatg tggttcaggt acgctttgta
    11761 ttgaagcagc cttaataggc cataatatcg caccaggatt caatcgtgag tttgcttgcg
    11821 aatcttggga ttggttcagt caagaaatca tggaaaatgt tcgatcagca gccgaagaaa
    11881 aagcagatta tgatattgaa ttagatatta ctggttcaga tatcaatgga cggatgattg
    11941 aaattgccaa agccaatgca gaagaaatcg gattaggctc ttcgattacc tttaagcaac
    12001 aagcagtcaa agacttcaaa acggataaag aatacggggt catcgtggct aatccgcctt
    12061 acggagaacg tctaggagaa gaagaaactg ttcggaaatt atataaagaa atgggcgatg
    12121 tattccgtcc attaaaaaca tggagtaaat atatcttgac tagcgactta gcgtttgagg
    12181 aattttacgg acaaaaagca accaaaaaaa gaaaattgta caatggtgca ttgcgtacgg
    12241 atctattcca gtattgggga actcgtccgc ctagaaaaaa agagaaagac gaataatctt
    12301 actaattgat tcattcaatt ggatgtttgg aggaataggg aatgaaagaa aaagagtttt
    12361 tacaagaagt aaaagaaatc aatcttctac aacaagcttt aggaatcctc gattgggata
    12421 cacaaacagg tatgcctgaa aaagctagtg cataccgaag tgaagtagac agctatcttt
    12481 acagcattta tttttcaaaa aaaatcggtc caaaaatcaa agaagccatt cgctattttt
    12541 ctgaacatcc ggaagaattg tcggaagttg gaactgccgt ttttgccaaa gtaaaagaag
    12601 agtatgattt ggaacatcgc gtaccggaag atcttatgag aaaatataca gctgccgttt
    12661 cttcagcaca cggacaatgg caaaaagcta gagaaacaca acaattttct gattttcaac
    12721 acgcattgaa tcaaaatatt gaattaacaa aacagttgat accttactgg agaaaagatg
    12781 aaaagacgaa ttatgatgtt ttgctgaatc aatacgaacc cgggatgact gtcgaaatcc
    12841 ttgataaggt atttgatcag cttaaagaag gaattctttc gatccgccgt acgctccaag
    12901 aaaaagggac tgaacctgaa acagactttt taaatcggag aatgacgaaa aaacaacaga
    12961 agagatttgt agtgaaagta atcgaacaac ttggttatga ttttagtcgt ggtcgtttag
    13021 atgacactgt ccacccattt atgactggaa ttaatccgaa tgatgcacga atcacgacac
    13081 gatggaacga aacagatttc aaaatggctg tttttggagt gatccacgag gctggtcatg
    13141 gtatgtatga acaagacatc gatgataaat atgcgtatac accggtttat gaaggtacat
    13201 caatgggtat ccatgaatca caatcgttgt ttaacgagat cattattgga agtagtcgca
    13261 gcttctggaa aaagcaatat cctttctttc aagaatgtgc agaaggaaca ttcgatgata
    13321 ttccttttga caaattttat cgttcattga accatacaaa atcaagtttg gttcgtatcg
    13381 aggcagacag tttgacttac cctctccata tcatcattcg ttatgaaatc gaaaaaatga
    13441 ttttcaatga aggggtagat gttgctgaac tacctgaaat atggaatcaa aaatatgaag
    13501 agtatctggg aatacgacca acaaatgatg tggaaggcat cctacaagac gttcattggt
    13561 caggtgcaag ctttggttat tttccctctt atgcattagg gtacatgtat gcggcacaat
    13621 tacttcattc tttgcagcaa gaaatagatg tggaaaatgt tctagcgtca gacgattatt
    13681 ctccaatcaa aaactggatg tcagagcata tccatcaata tggtgcatct agaaaaccaa
    13741 atcaattaat catggatgca acacatgaac cgcttaatcc gcaatacctg atcgactata
    13801 tgaaagacct gtattttaat gtatatcagg taaaataaaa tacaatttac aaagatagct
    13861 gaaattcatc taatctatag atgttttcag ctatttactt aagaaagaag tggaacgaat
    13921 ggataaatta caagtgatcg ctgattatag tatggaccaa ttagcgcatg atcaaacagg
    13981 acatgggagc gatcatacca aaagagttgt caagttagct gaaaaaattt tggagacgga
    14041 accgcaggca gaccgctttg tgacgcttgc agcagcttac ctacatgata cgattgatga
    14101 taaagtcgtt aaaaatgaga acgaggcaaa gcaacagctt cgtgtattcc ttcgctcttt
    14161 gccaatagta gaggaacaga tctcgatgat tttttccatt atcgaaaata tgtccttttc
    14221 taaaaatctc tctgaagcgg ccgaactatc tctggaaggt aagatcgttc aagacgctga
    14281 tcgtatcgaa gcattaggag caatcggcat tttgcgtact gcttatttcg gcggtggaca
    14341 tgggcacccg atttttgatc ctgaactgta tccgcaaaca ttcaaggaca aagaaaatta
    14401 ccgaaaaggc acgacagtga tcaatcattt ttatgaaaaa ctgttcttgc tgtctgataa
    14461 aatgaatact gattacggat atgaagaagc caaacgaaga gaaaccttca tgcgtgaatt
    14521 cttagaagaa ttttttctgg aatggtctat tgacgatgaa aattttactc cagacaattg
    14581 gatcaaaaat aacaaaaaat gatggaggtt gtgacagaag tggtcagctc caagatattt
    14641 cggatcattt tccgtaaaag ttacttctgg aacactgttt accaagaggt cgtgagcctg
    14701 ccgttcagct ccgagtcaca aggaacaatt tcaaaaaata gcttctcgtg tttgatgaat
    14761 attgtaactt gtgattcgga attggcagga gaacaccgtt tattcggaag aataggactg
    14821 tgacaagact tttgtcacag tccttttttc gaattcaaca agtaaaactt ttaagaaata
    14881 ttcagagagt aaaattaaaa attgctgata aacgaagatg gaaattttat gtatttttat
    14941 tggtctatgc aataaataaa ttaaatattc agaatacaat tgattttaac gaaagggctt
    15001 ctatcaatgg ttgtgttgta tttatctgcc caggtagagt aaaatagatc attatctaaa
    15061 aggtaattgt tcatactata taagtcattt gaaagtaaaa agatactgta aataaaaaat
    15121 gtggttaaat aattctcata atcagtgtaa gcgttatcaa aaatatagca tacttgcttt
    15181 gtagatgaat gacacgatat atgtttggta tgttctgcaa gacagatgaa tatggaggtg
    15241 gagacaatgt cttccaaaga actaacaatg tatttagaga aagaattgga tcaactgaaa
    15301 gaaaaggggt tatacaatac gatcgacata ctagaaagtg aaaatggtgc ttgtatcatc
    15361 gtagacggaa aaaagatgat caatctagca tccaacaatt atcttggatt tgccaatcgc
    15421 gaagaattga aaaaagcatg tattgaagca accgaaactt atggtgtagg agcaggagca
    15481 gtccgaacga tcaatggttc gctgaaaatc catcagcaat tagaagaaaa aatcgcagaa
    15541 ttcaaaggga cagaagcagc aatcgccttt caatctggat tcaactgcaa tatgggcgct
    15601 atctctgcag taatgacaaa agaagatgct atcttatcgg atgaattaaa ccatgcatct
    15661 attattgacg gttgccgctt atccggagca aaaatcatcc gaattaaaca tcaagatatg
    15721 aaagaccttg agaaaaaagc aaaggaagcg atagaaagta agaaatataa aaaaatcatg
    15781 tatatcacag atggtgtttt ctccatggat ggagacattg cacgattgcc ggaaattatt
    15841 ccgattgttg aaaaatatgg actgatcacc tacgtcgatg atgcgcacgg ctcaggcgta
    15901 actggaaaag gtgcaggaac cgtcaagcat tttggattaa gcgacaaaat cgatatgcaa
    15961 atgggcacgt tatcgaaagc tataggtgtt gttgggggtt atgtagctgg atcgaaaaca
    16021 ctaatcgact ggttgaaagc tagaagtcgg ccattcctat tttctacttc gctaactcca
    16081 ggtgcagctg cagctgcatt ggcttcgatt actttgatgc aagaacatcc ggaacttgtc
    16141 gaaaaagttt gggaaaatgc taattatttt aaagaagaat tgaaaaaagt aggctataac
    16201 atcggtatgt ctgaaacacc tattactcca gtcattttgg gagacgaaaa agtgactcag
    16261 actttctcta agaagctgat cgaacatggg atttatgcta aaccaatcgt ttatccaact
    16321 gtgcctcttg ggacaggcag gatcagaaat atgccgactg cggaacatac gagggaaatg
    16381 cttgatgaag cagtagcagt ttatcaaaaa ataggaaaag aaatggaaat tatttaacta
    16441 aatttagttt tttcgtctat ttaaaattag agaacatgtt cacgcatata ttgttaatca
    16501 tctgaagata gtagtaaaaa agatcaggga ggatcatcat gaaaaaagta ttggtgacag
    16561 gatgtttagg acaaattggt tcagagttga tcacacggct acgtcaagaa ctaggcagtg
    16621 aaaatgtcat tgcgacagat atacgcatgc ctgaagtcat tgagaaaaac gatatctttg
    16681 agatcctaga tgtaatggat tacgaaaaaa tgcgcagcat agcagaacaa tatcaagtgg
    16741 acacattgat tcatttggct gctcttttgt cagctgttgc agaagcaaaa ccacaattag
    16801 cctggaattt gaacgtgact gggctggtca atgcattgga agtcgctaga gaattccagc
    16861 tgaaattttt tacgccaagt tcaatcggcg cgtttggtcc aaatactcca aaagatcata
    16921 ccccacaaga tactcttcag cgtcctacaa ccatgtatgg ggtaacgaag gtagctggag
    16981 aactactatg tgattattat tatgtgaaat atggcgtaga tactcgtggt gtccgtttcc
    17041 ctggattgat ttcttatgca accttgccag gaggcggtac gacagactac gcagtcgata
    17101 tttattattc agcaatcaaa gaaggaaagt acactagttt cattgatcaa ggtacatcaa
    17161 tggatatgat gtatatgcca gatgcgatcg atgccattat aaaactcatg aatgcagatc
    17221 ctgatcgatt ggttcatcga aatgccttca atatatcggc gatgtctttt gacccggaag
    17281 agatcaaagc atctattcaa aaaatcatac cagattttga aatggattat gatgtggatc
    17341 ctgtacgtca gtcaatagca gaatcttggc caaattcact agatgtttct tgtgcgcaag
    17401 aggaatgggg cttttcgccg aaatacgact tagatgtgat gacaaaggat atgttggaaa
    17461 aactgaaaga aaaattggta gagacggtat aaaacaagaa ccacatccaa aaagtttttt
    17521 atacttttgg gattggctat ggtatgaaag gggacaaggg atggaagaat atcaagccac
    17581 gccagtaaaa gaagtaaaaa tagaaaatga attaaaacgt acgatgggat tttttactgc
    17641 tttatcgact gttatgggca cagtgatcgg tgcaggggta ttttttaaag ccgcaagcgt
    17701 agcagaagtt acaggatccg ccagtttgca tatgttttct tggtttctcg gtggtatgat
    17761 ttctgtctgt gcaggactaa caggagctga actggcagct gctattccag aaacaggagg
    17821 gatgattaaa tatatcgaac ggatttacgg aaatacagca gcttttttat tgggctgggc
    17881 gcaagtcgtg atttattttc cagcaaatgt agcagccttg tcgatcattt tcggtacaca
    17941 gttcgtcaat ctatttggtc tttcccagtc aatgattgtt cccgtagctg ttacagctgc
    18001 tgtatcgatc ctgttgatca attttttagg ttctaaagct ggaggggctt ttcagtcgat
    18061 tacgcttgtc tgcaagctta ttcctttgtt tgtgatcgtc attttcggtt tgttccgaca
    18121 agaaggagta gacttccaac tgtttccgat ccaagcagga gaaaatcttt catttttctc
    18181 agccttggga gcgggcttgc tagcaacgat gttcgcatac gacggttgga tacacgtagg
    18241 aaatatttcg ggagaattga aaaagccggc gaaagatttg ccaaaagcaa tttctttagg
    18301 aatcatcgga atcatgatcg tttatttatt agtaaatgcg gtgttcttaa gaaccgcatc
    18361 tatcgacgga gtcgcaggaa acagcaatgc ggcaagtgac gttgcaaaaa tgatttttgg
    18421 cggcttcggt ggaagactgg tcacagttgg tatcttgatt tctgtttatg gaaccatcaa
    18481 tggctatacc cttactggaa tgcgtctccc ttatgttatg gcaaaagaaa acaacctgcc
    18541 ttttagcaag ctctttgcta aacttcatga taaaactaaa gttcctgtag ccgcaggtat
    18601 cttagagcta gtcatcgcta ttggcatgat gatgatcggt ggttttgata cactaacgga
    18661 tatgctgatc tttgtcattt ggatttttta tacgatggtc tttgttggtg tcattcttct
    18721 tcgtaaaaaa gaacctgatt tgtttcgacc atacaaagtt ccaatgtatc cgtttattcc
    18781 actagtagca atcattggag ggacttttat cgtatcaagc acgcttatca ctcagacctt
    18841 tttagcttcg atggggatag tattgacact tgctggtatc ccgatttatc tctatttaaa
    18901 acgtagatag ctaaaacatt cgaattttat tagaactgca cttctctgcc aaacaaaata
    18961 ttttatttaa atgaggagta cgtggaagaa atgtcaaaag tactcgttaa ttttgatcac
    19021 gtaaatgtcg ttttgtctac gatgaaatag caaagtaaaa gacagaaatc gggctcaacc
    19081 gatttctgtc ttttactttg atttgcgtaa ctgaactatt cggcgtacgc cgcgataaat
    19141 atttaataaa accaagcaac cagaaatgaa aatgggaagc caaatcagat agacagggta
    19201 gccaagcact ttagacaaag catgtggagt cattttatcc atttcaagaa gtgcttgctg
    19261 ccaagtctga agattgaaga ctagcatgaa acccgaaata atcaggagaa taaatcctaa
    19321 ataatgaatt gtagttcttt ttcttacata agcgatcgta gcataaagga acagtaaaaa
    19381 aataggtacg ccaaaaacaa agtacatatt cagcatctcg acaccccctt tgtgtttatc
    19441 ttactttact ttgaaagaaa tgagaaataa tagcctcgtt acttgcttga aacagcaaag
    19501 gactgctaag ataagagaga tacaaagaat caaggaggag tcatatggca agttatatcg
    19561 aaccgattcg ttttgcgctg attgtttttc catttttagc cttagcgatc tcatctgttt
    19621 tttttattta tgaatatcga aagtatggaa catttctagt aacaagggca gttattttgt
    19681 attctttcgt tttttattta ttgtgtgcgt attttttggt gatcttacca cttccggcaa
    19741 aggagacggt tgcccaaatg acagggccaa agatggagct gcatttattt gcttcatggc
    19801 aacatttcag agacaacacg gtattggtct tgtctgaacc tagtacctac ctgccggcaa
    19861 tgaagcagag tgtatttttg gaaccagctt ttaatgtttt attactcata ccatttggca
    19921 tttatttaag atattatttc cggctttctt ttatgaaaac gatgattgct agtttctgcc
    19981 tttctctatt ttttgaattg acgcagctaa gtggacttta ttttatctac ccaagaccat
    20041 accgcttatt tgatgtgaat gatttagtga caaatacatt gggtggaatc attggttttg
    20101 tcgttacgcc gttatttaca tttatgcttc ctacaagaag acgaatggat gaactttctt
    20161 atgaaaaagg aaagactgtc tcattgactc gacgcttggt cgcttatttg atcgactgga
    20221 catttttatc tcttattaat ttggtagcgg cgattttctt taaaattgcg ttcgattcag
    20281 gggcagttac ttctcatatc tggtggcttt tattagaagt aggggtatac tttatgattg
    20341 taccgtttat aaccaatgga tggacacttg gcaaaaaaat tgtgcgtatc cgtttggtgg
    20401 aagaagaccg cgaacgaatt tctttaaaag cattgatgat ccgctacggg tatctgtact
    20461 ttctttttta tggtttaagc tggttggggc agttgcttca gcaaatcatg aattcgacaa
    20521 cgaatacggt gttgcagatg agtgcagtat tcatttctct cggtgtcttg ttactacaag
    20581 gtatttttat tttaaatatc ttgctatcat ttttgagaaa aaaacggatt ctgttttatg
    20641 aaaaagctag tcacacatat actgttagca cgattggcca aacaaaggat gatgattctc
    20701 gaaattagct gagcaaaaac gctttgtctg tgttataatg ccaaagtcgt gataatttaa
    20761 agagaagagg tgcacgaatg aataattttg atatattccg cttcaaaccg tttatcaatg
    20821 aagcgttaac cgcaaagggc ttcaaacagc ccacagaggt ccaggaacgt ttgattcctt
    20881 taattgcaaa aggaaaaaat atcattgggc aatctcaaac tggtacagga aaaacacata
    20941 cgtttttgtt gcctttgttt gaaaaaatca atgcagaaaa agaggaagta caagttgtta
    21001 ttacagctcc aagtagggaa ctagctgcac agatctatga agcagctaca caaatcgcta
    21061 aattttctac accggagatc cgtgtctcga attttgtcgg cggtacagac aaacagcgtc
    21121 aattaaatcg tctgcatcac caacagccac aggttgtagt aggcacgcct ggtagaatat
    21181 tagacatgat gaatgaacaa gcgctgaaga ttcacaccgc atttgcattt gttgtcgatg
    21241 aagcggatat gacattggat atgggttttt taaatgaagt agaccaaatt gcaagtcgat
    21301 taccagaaaa gctgcagttt ttagtttttt ctgccactat tccagaaaaa cttcgtccat
    21361 tcttacgaaa atacatggaa aatccaatca ttgaagaaat caaaccaaaa gcagttattt
    21421 ctgaagacat tgaaaattgg ctgatttcaa caaaaggtaa agatgagaat caaatgattt
    21481 atcaactgct gacaattggt catccttatt tagcaatcgt atttgccaat acaaaacagc
    21541 atgtggatga aattgccgat tacttgaaaa accaaggctt gaaagtagca aagattcatg
    21601 gagacatcac cccaagagag agaaaacgag tgatgcgaca agtacagaat ctggagtatc
    21661 aatatgttgt agcgactgat ttagctgcta gagggatcga tatcgaaggt gtctctcatg
    21721 ttattaatgc agaaattcca gaagatctag acttctttat ccatcgtgtc ggacgtactg
    21781 gacgtaatgg cttaaaagga acggccatta cgttatattc gccaaatgat gagcaagcca
    21841 ttgatgcgat agaaaaattg ggcgtctcat ttgaaccaaa agaaatcaaa aacggggaaa
    21901 tcgtcaaaac atacgatcgt aatagacgtg ccaaacgaga aaaatcaaaa gatgtattag
    21961 atcccacgct gattggtctt gtgaagaaga aaaagaaaaa gatcaagcct ggttataaga
    22021 aaaaaatcga ttgggcaatt gcagaaaaaa ataaaaaaga acgcaaaatc gaacgcagac
    22081 agcaaagccg tactgctcgt aaaaataaga aaaattcaca taaataaaaa acaagaggtt
    22141 gtgacaaaag gcgtgtcaca atctcttgtt tttaagctag actttttttc attccgatac
    22201 aaagtacttc atcttcaata aaaggttctg tgcaagtatg atacccttgt ttttcataaa
    22261 aatctacagc cgtcatttca gctactacat aactagtctg acaaccttct gaaaccgccc
    22321 gtttttcagc agcttcaagc aactgaccgc ctaacccttt tcctcggtat tcttttgcta
    22381 cgcaaaatcg ttccggcttg aagcaagtag gtgttttctt ttcataacgc aaagtagcta
    22441 ctggtctttt ttgatcaaga aatagataat aggtttgttg cgggatatct tttccgtcga
    22501 attctagttc tgcaggaatc ccttgttcta atacaaaaac ttgatatctc aaaaagcagc
    22561 tagctgcgcg tatccattca tccgctccaa ctattttttt catcatattc ctccttgtca
    22621 tgaatagcag agatgtattg attcctttat tcttatcgta ccaaaaatcg tggagtatga
    22681 ccaaaaggaa atgaaaataa aaattgagat attgacagtg atcaatagaa tgcgtatact
    22741 aattaagtac gaaaaggata tgatttgttt tttaagactc acagaaaatt ctttcttggc
    22801 tgaaaaaaga atagtcacta atcaaattgc tccctttttc tattttggta gaaataccga
    22861 cgcagctcag cgttatcgag tttaagaggt aatgattaca catcattgca atcaaggtgg
    22921 taccgcgagt tttcgtcctt ggctgtgatg gtgttttttt gtatttaatt tatagaaagc
    22981 aggtaatgac atgaaacaat taagtagtgc acaagttcga caaatgtttc ttgatttctt
    23041 ccaatctaaa ggacatacga ttgaacctag tgcgtctttg atccccgtga acgatccaac
    23101 cttattgtgg atcaactctg gtgttgcaac attgaaaaaa tattttgatg gttcagttgt
    23161 gccagaaaat ccaagaatca caaatgccca aaaatctatt cgaacaaatg atatcgaaaa
    23221 tgtcggtaaa acagcacggc accatacgat gtttgagatg ctgggtaatt tttcgattgg
    23281 tgactatttc aaaaatgaag ccatccactg ggcatgggaa tttttgactg atccaaaatg
    23341 gatggcattc gatccagaaa aactttatgt aacagtttat ccaaaagata cagaagcaaa
    23401 acgcatctgg catgaagaag ttggcctgcc actagatcac attattgatg tagaagacaa
    23461 cttctgggat atcggtgcag gcccatgtgg tccggatacg gaaattttct atgaccgtgg
    23521 agaagcttat aacgatttag cagaagacga tcctgaaaac tatccaggag gagaaaatga
    23581 acgttattta gaaatctgga acttagtatt ttcagaattc aaccataagc ctgataacac
    23641 atttgaacca cttcctcata aaaacattga tacggggatg ggattggaac gcatggtctc
    23701 aatcgttcaa gatgcgtcaa cgaactttga gacagacttg tttatgccta ttatccacgc
    23761 cgtagaaaag ctaagcaaac aagtaaaata tggtgaaaac ccagtaacag atatttctta
    23821 caaagtaatc gcagatcata ttcgcgccct ttcatttgct attggcgacg gtgcattgcc
    23881 atcgaatgaa gggagagggt atgtcttacg ccgtttgctt cgtcgtgctg tcatgcatgg
    23941 gaaaaaatta ggtattgaac aagcattctt atatcgttta gtccctgttg ttggggaaat
    24001 catggtaagt tattatcctg aagtacttca acaaaaagaa tttattgaaa aagttatccg
    24061 tacagaagaa gaacgtttca atgaaacaat caatgaaggt ttggaaatct taaatcaagt
    24121 gatccaagaa gtaaaaaacg aaaacgggac gactctagca ggaaaagaca tcttcaaact
    24181 atacgacaca tatggcttcc cggtagaatt aacggaagag gtggcagaag atgaaggact
    24241 gaaagttgat catgctggtt ttgaaaaaga aatggatgca caacgcgaac gtgctcgctc
    24301 tgcccgcagt actgaaaaat caatgggggt acaatctgct ttattgacag atatcaaagt
    24361 agaaagtaaa ttcattggat acacagaaca agaatcagat agtcaattat tggtcatcgt
    24421 taaaggtgag tctcttttga atgaagtcgc agaaggtgaa gctcaaatga tctttgatca
    24481 aacacctttc tatgcagaaa tgggtgggca agtagcggat caaggatgga tcatgaatga
    24541 agtaggcgaa atcgtagcag ttgttacaga tgttcaaaaa gcgcctaatg gtcaatttat
    24601 gcacaccgta gaagtaaaag caccaatgag tgaaggacaa acctatcatc tattgatcga
    24661 tcaatcgatg cgcaatcgga tcatcaaaaa ccatacagca acccatctac tgcacaaggc
    24721 attgaaagaa gtacttggcg agcatgcaaa tcaagctggt tctttagtcg ctcctggaca
    24781 tttacgattc gactttaccc actttggtca aatcacttca gatgaattag ctgaaatgga
    24841 aagaatcgtc aacgaaaaaa tctgggaagc tatcccagtt gaaacaattg aaacagatat
    24901 cgatacagcc aaaaacatgg gtgcaatggc tttgtttggt gaaaaatacg gacatgacgt
    24961 tcgagtagta aatattggcg gctggtcaat cgaactgtgc ggaggcacac atgtagcaaa
    25021 tacagaaaac atcggaatct tcaaaatcgt ttctgaatca ggaattggtg caggcgtgcg
    25081 acgaatcgag gctgtaacaa gtaaagaagc gtacgaacta cttgaagaag aagaaaaaca
    25141 actaaaacaa gtagcaacaa ttgttaaatc acctcaattg aaagaagttg tttcaaaagc
    25201 ggaacaattg caacagcaag taagagagct acaaaaggaa aatgaacaat tagctagcaa
    25261 gttagcgaat caacaagctg gtgatgtatt caaagatcca gaaacgatca atggaaaaac
    25321 aatcattgca gcacaagtaa aagtgaaaga tatgaatcag ctgcgtcaat tagctgacca
    25381 atggaaacaa aaaaatattt cagatattct tgttttagca aacgaacaag aaggaaaagt
    25441 cagtctgcta gcggcaatga cgaaagaatc aaatgaagct ggactaaaag ctggcgactt
    25501 gattaaagca atcgcaccga aagtaggcgg tggcggtggt ggacgtccag acatggcaca
    25561 agctggcggg aaaaatcctg ctggtattcc agatgcactt aaagctgcga aagaatgggt
    25621 tgcgcaacaa tagggcgttt acgtttgaca gaaaaacaaa agttcaaaca ttgaacgaaa
    25681 catgattatg ggggcgaaga caaacaagtc ttgccctcat acttttttca attggatggc
    25741 gcatctaatc aaatggtcgg attcggatgg tctttagttg acgaaagtga cagaaatttt
    25801 tacgattagt ataaataaaa aggggtgaaa aaatgaatag tagaaataag acctttcgac
    25861 tagtcttacg tgcgatcttg ttagcaatca tcatcgtaca agcgatggtt ccttggctgg
    25921 ggtttattcc attaggattc attagtttaa caattatcca tatcacagta attattgctg
    25981 cagtagtatt aggcccaaaa gatggcatgg tgattggctt gttttgggga atcgctacaa
    26041 ttgttcgtgc ctatgccatg ccaacaacgc catttgatac attagtgttc acgaatccga
    26101 ttatctcggt agttcccaga gtacttgttg gcttagtagc aggcttggtt ttccactgga
    26161 tttatcaaaa aaataaatca ataacgatca gctcagcctt tgcaggggta ctgggttcat
    26221 tggtcaatac agtcttagtg ttaggattca tgggattatt ttatacaggt gcaacggcag
    26281 atgcctatgg tgtagactct gctcttttat ttaaaacctt agcaggggtt gcagcaatca
    26341 atgggatacc agaagcaata ggtgcgggaa tcataacacc attactagca aaagcgttat
    26401 ttgcagcaac accgttgaaa ccagaataat gaccatcttt taatagtaat aggacgcacg
    26461 atagatagaa aaaggaagag aggagaggaa aacaatcgtc ctctccatct ttttacctga
    26521 gcaacaagca aactagaagg ggtaaaaaac ttggctaaga aacaagaaga aaaaaagaaa
    26581 caaataccgc aacagcggac aaaaaaagga gtactagcgg gtatcttagg attggttacg
    26641 aatctgatct tgtttgtctc taagctaatg atcgggcttt tttcaggaag tgtctccatt
    26701 atggctgatg cagtgaacag tctatcagat actgcttcat ccatattgac tttgatcgga
    26761 tttaaaattg ctgcaaagcc agcagatcag gaacatcctt atggacacga acgcttcgaa
    26821 tatatcagtg ggttatttgt ttcgatcatc attacttatg taggatttca atttttagag
    26881 tcttctgctg aaaaaatcat ccatccagaa aatgtctctt taacacccat cgtctttttt
    26941 gtcctaatca tttccatctt tttgaaattt gcacaaggca aaatgtatca ggttattgcg
    27001 caatccattg attctgaaac attgagagct acagcaacag atagttataa tgatgtcttt
    27061 actacactgg ctgtattgct ttcagcagca atcgaatggg taactggatg gcgtattgat
    27121 ggatatatag gttttgcttt agcattatat atcctttata gtggattgac gatgcttcga
    27181 aattttgtat acgaactatt aggaagtcgc ccaacgccag cagagattga agaaatggaa
    27241 gagcatttaa atgcttgcga tgatatactt ggctatcatg atttattggt gcataactat
    27301 ggacccaata aacgctttgc atcagtccac atcgaagtag atgaatcaat gaatttgaat
    27361 gtggctcata agattattga tctgatagaa aaagacttta aaaagtcttt ggatgtggaa
    27421 cttgtctgtc acttggatcc aatgcctgtt gataacgaaa aatactataa tatcttgcgg
    27481 cagttaaagc aaattcttct tgaaacaggt acagggctta atattcatga ttttagagta
    27541 attcatcaag gaaaaacgct gcaatttgat gtagtggtcc ctgagaaatt ccagtttgtt
    27601 gatacagaac tagaaagaat cttacgtagt caaatagaag aaaaaatcgg agattaccaa
    27661 ttggaaatta cgtttgatca caattatctt ttatattaaa tgagaaacta taaaaagact
    27721 ccttaaagtt tttaatttaa agagtctttt tttcttttaa ttgtttatgg agagcctgac
    27781 gtgcaagatt atctgctcct ttatttttgt tttcgggcaa ccattgcagg atcaataaag
    27841 aaaattcgga tagaagttct tggattcttt gaaggtaggg aaggaagagc tcgttcttcg
    27901 ttgcgttttt gtcgaccgta gaaactagtg ttttgctgtc tgaaaaacag aaaatcgttt
    27961 cattctgtaa gtcgtttttc tttagaaatt ctaaagtttt taaaaagact tcgaactctg
    28021 cttgatggtt tgtccctata gaaagcggga tagatagctg ttcttgttta ccttgataga
    28081 caatcaagat tccacctcca ctaggaccag ggttgccttt tgtagaggca tcaatgtata
    28141 cttttaacat aaaaagagat tctccttttt gttcaaacta atgttatggt aagattatat
    28201 cattactgaa tcggtgagag gttagagaat gaaaaaaaat attcgatggc agcctgaact
    28261 ggcacatacg atcatctatt ggtcattgac cttgatcatt ctgtttttta gtctgattct
    28321 ttctttagaa aacacaggtc cttattggaa aagcaatttt gttttaggta tttttcttgt
    28381 ttttctattt ctaggtttgc ggcgttcttt tattttaaaa tcaaactctg tgattatacg
    28441 ttatgctgct ttttggaaag accgagaaat atctgtcgga cagattcgat ctttgacgcc
    28501 aactaatact ggggtgcaaa tgatgttgca agatgagaaa gccccatctg ttttcatgat
    28561 gcgaaaaaaa gatcaagtag tcctttttga tcattttgaa aaaaacggac ccaaatcaga
    28621 atcaattcct tatccttata cgaaaaaaag aaaaagctaa ttttaaaaat cagaacctaa
    28681 aaaaacgaat ttttacagga aaagaaaaga gtgagacgta cggttcatcc cgtttagcgt
    28741 ctcactctat ttgtttatct attcatgcat actagtatcg gtagtctgaa cgttgccttt
    28801 cttgacatgg gcagcttgtg ttcctcgatt cccttcgatg atctcaaatt cgaccacttg
    28861 attttcttcc aaagttttga atccctcttc ttcaatcgca gtaaaatgta caaaaatttc
    28921 ttctgtgtca tcataactga taaagccgta tccttttttg ttgtcaaacc attttacaat
    28981 tcctgtggtc aaagcactca cgacctttct aaagtctgat catagccttc attataaaga
    29041 gtatttataa gtacgtcaaa taaattaaaa acctgtaatt ttatgttttt tttctgaata
    29101 aaaaaaggta taattggcat atcttaacaa agaagatatt ttgttgaata aagttcgtga
    29161 aaaggggtaa tcaaatgaag aatgatctgc aaatttcaca agaaacagag ctagaaccga
    29221 ttgttgaagt ggctagtaaa ttagatctta aggaagacga tttggaactg tatgggaaat
    29281 ataaggcgaa aatcaatttt tctggaatca atcgatcaaa ggataacgct gaggggcatt
    29341 tgatcttagt tacttccatc aatccaacac cagctggcga aggaaagtca acgatcactg
    29401 tagggttagg agacgcgctc aataaaatca ataaaaaatc tgttattgca ttgcgagaac
    29461 cctcacttgg tcctgtgatg ggaatcaaag gtggtgctgc aggcggagga tatgcacagg
    29521 tattgcctat ggaagatatc aacttgcatt ttactggtga catgcatgct atcacaacag
    29581 ctaataatgc attatctgct cttttagata atcatattca tcaaggaaat gaattgaaca
    29641 tcgatgcccg ccgagtgatt tggaaacggg tagtcgatct caatgatcgt gaactgcgta
    29701 aagtaattgt tggcttgggt gggccaatcc aaggtgttcc aagagaagat gggtttgata
    29761 tcacagttgc cagcgaaatc atggctatct tgtgtcttgc ggcagacctt gaagatttaa
    29821 aggctcgtct tgcaaggatc gttgtcggct atacatttga ccgtcagcct gtgactgctg
    29881 gagacttgaa agcagaaggc gctctggcat tactgctgaa agatgcaatc aagccgaatc
    29941 ttgtacaaac aatttacgga acaccagctt ttgtccacgg gggaccgttt gccaatatcg
    30001 ctcatggctg caatagtatt ttagctacca agacagcatt gagattagca gattacgttg
    30061 tcacagaggc aggatttggt gcagatcttg gcggagagaa atttttagat atcaaagtac
    30121 ctaacttgaa aaaagcgccg gatgctgtgg tgattgtcgc cactgtccgt gcattgaaaa
    30181 tgcatggcgg aatgaaaaaa gatgaactta aaaatgagaa tcttgatgct ttgaaagtag
    30241 gttttgcaaa tttaaagcgc catatacgta atatggaaca atatcagcta cctgtgatcg
    30301 tcgcaatcaa tgaattcgta acagatacag acaatgaact cacactgttg gagcacttgt
    30361 gtgaaaatca ggggattctt gccaagagag cgagtgtatg ggcagatgga gcagaaggcg
    30421 gcgttgattt agcaaaagct gtagtacgat tgattgatag aaaagaagct aattacaagc
    30481 cattataccg cctagaagaa acgatacaag aaaagacaga aacaatcgtc aagaaaattt
    30541 acggaggaaa tggtgttgtt ttcagcaaaa aagcgcaaaa acaaatagaa gagtttacga
    30601 agaatggatg ggacaaactg ccgatttgta tggcaaaaac acagtattca ttttctgatg
    30661 atccttcact gctaggtgca ccggaagatt tcaccatcac gatccgcgaa atcatcccaa
    30721 aattaggggc aggattttta gtagccttga ctggagatgt tatgacgatg cctggtttgc
    30781 ctaaaaaacc tgctgcattg aatatggacg tgacaaatga cggagaagtg ttgggattgt
    30841 tttaagcttg attataatct accatcgaag gagctgtatt ttgactacag cttctttttt
    30901 tagacgatag ggaattaatt ttgtttacac taggatcatt ctttttcttt attgtatttt
    30961 gagctatact aaagcataat aggagggtat gatgaaaacg acatttataa gaaaaaccgg
    31021 tttttacggt atgggtagct cgattcagct aagaatcaac aatgaaaaac gcagtgttct
    31081 cagtcatgaa caaaagataa cgcttgatct ggaagtgccc tttacgatgc aggtcacctt
    31141 ttattggcta aaaagccccg tttatacagt gaaagaagca aagcctgctt atgtgatcac
    31201 aatgaattac ttgattcttc aaatctatcc gtttttattt ctagcaactg ggttgggggc
    31261 cgttttccta caaaatttat ttttcagttt ttttgcagtt attgtgttga ttgcgttctt
    31321 tttctatata aaaaaccggg catatttgat caaggaggaa aattatggca aactctgatc
    31381 tttttttaat cagtttcaat cgaatcgaaa aatggatgag agacgagatg ggaaatgcac
    31441 gaaatatggg atttaccgaa cttgtcagac gcctagctca aagaaaacaa ttaatgatca
    31501 gaaaatacga agatgatctt ttacagcttg ctcagttaag gaatgcgatc gtccatgatc
    31561 ggatcgcagt cgattttgtc attgcagagc caaatgattg gacagtcaag cgaatccagc
    31621 tgatcgaaca agaactgatt cagcctgaaa ccgtgctgcc tcgatttgca aaacgtgtaa
    31681 caggttttga acaagatctt ccgttacttg aactgttaaa gatagtagca gaaaaaaggt
    31741 attcacaatt tccgttatac aataaaggaa aatttgtagc attgattact ttacgtaata
    31801 tcgggttttg gctagcaaaa gaaagccaaa aaggtccggt tgatttaaca aataaacgag
    31861 cgttggattt gatcatccaa aacggaaagt acacaaatta tcattttgtt ccagctaaaa
    31921 cacatattta cgaggttgaa gcgatgtttc gtgagcaagg gactttggaa gccgtactta
    31981 tcactaaaga tgggaatccc gatggtaatc tgctcgggat cgtacgacct cgtgatattt
    32041 ataaacaaat agaaaaggat tgattttttg ttaattattt attggatatt cagcgctttg
    32101 ctagtaggag tggatcagtt ggtcaaatgg tggatcactg ataatctagc gcttggagag
    32161 acgaaaaatc ttataccagg tattttgtca ctgaatcata ttcgaaacac tggagccgca
    32221 tggagtatgc tagaagggaa gatgtggttc tttactatag tgacactcat tgcagtcgtt
    32281 gtgattctca cattgatgat taaaaatcgt gaaaaaggta atcgctggtt catgattgga
    32341 ttgagtttga ttctagctgg tgcgattggc aattttatcg accgtattcg tcttggctat
    32401 gtagtagata tgttccagac agattttatg agctttccga tttttaacgt agcagatgtg
    32461 acacttgtat gtggagtgat ttgtatgtta atttatatta ttttagatga aaaggatcaa
    32521 agaaaaaaat gacagaactt aaaacgacga ttcaccatga atctggtagg atcgataaag
    32581 tattgactaa tttattcacc gattacagtc gttcgcaaat tcagctgtgg ctgaaaaatg
    32641 gagcggtttc tgtcaatgga cagccagtca aagcaaatta caaagtaaaa caaggagacg
    32701 agattactgt cttgatcccc gaaccagaaa cattagatgt ggtcccagaa aatatcccgt
    32761 tgacgatcgt ttacgaagat gaagatgtag cggtgataaa caaacctcaa ggaatggtcg
    32821 ttcatccttc agcaggtcat acgcatggca caatggtcaa tgcgctgatg taccatatca
    32881 aggatctttc tacgatcaac ggcgttattc gtccaggtat tgttcaccga atcgacaaag
    32941 atacttccgg actgctaatg gttgccaaaa atgaccaggc acacgaatca ttagctcagc
    33001 aattaaaaga taagacctct ttgcgcaaat atgttgcttt agtccatgga gagattcctc
    33061 acgaaaaagg aaccatcaat gcgccaatcg gccgttcgaa ggaaaaccgg aaaatgcagg
    33121 cgataagaga agatgggaag tcggctgtca cacattttac tgtattggaa cgtttcgaag
    33181 gatttactct ggttgaatta aaactagaaa ccggaagaac acaccagatc cgtgtgcata
    33241 tgaagtatat cggttatccg ttagctggag atccagtata cggaccgaag aaaactttga
    33301 aaggcaatgg tcagtttctc catgcgcaaa tgttaggttt tacgcacccg actacaaacg
    33361 aaaagatgat ctttgaagca cctcttccag aagtttttga aaaaacttta gaaaaattaa
    33421 gaaaaaacat tgcattttaa tttagataac ggtatagtaa ttaacaacga aagcataaaa
    33481 cttaatagaa ttcctttaag tttagtcccg tgaggctaag aagggtggga gcaaggatac
    33541 gtatggtgcg ccataagtag attgctcatt gacatagtat agctatcagt gtatcagtat
    33601 acgctcagtc taaacattcc tcctgtcctc ttagggcagg agttttttta tgcaaaaaag
    33661 tagggggaat gaaaatgcaa gcaaaagaag tagtagatca ggtaaccatg aagcgagcat
    33721 tgactcgaat tacttatgag attatcgaga gaaaccatag tatccaagat atcgtattgg
    33781 taggtatcaa aacacgagga atctacattg catcaagaat tgccgaacgc ctgaagcagc
    33841 tagaagatat cgatattcct gtaggagaac tggatatcac tttataccga gatgacaaaa
    33901 aagaaaatcc agaagaaccg gaacttcatt cttctgacat cccagtttcc ttagaaggaa
    33961 aagaagttat tttgattgat gatgttttgt atacaggacg aacgattcga gcagcaatgg
    34021 acgcagtaat ggattttggc agaccaagaa aaatttcatt agcagtcctg gtcgatcgtg
    34081 ggcatcgaga attgccaatc cgtgcagatt atgttgggaa gaacattcca acatctcggg
    34141 cggaagagat tttagtagag atgcaagagc ttgatggaca agatcgcatc atgattttga
    34201 aagaggaaga ttagaggaga gagaaacatg tcggagaaaa aaggatttca taatgatgac
    34261 gtggtgttag atatccacga tcgcccaacc ccaggacatt gggtaggatt aagtttacaa
    34321 cacttgttca cgatgttcgg agcaaccgtg ttagtcccca tcttagtagg tatcgatcca
    34381 ggtattgcgc tagtcagttc aggactaggt acattggtgt acttatttac tactaaagga
    34441 aagatacctg cgtacttagg aagcagcttc gctttcattg cagcgatgca gcttttgatg
    34501 aaaacagatg gttatccagc gattgcacaa ggagcagtca caactggatt agtctatttg
    34561 attgttgcgc tgatcatcaa aaagatcggt tcagcatggc tggataaaat cttgcctcca
    34621 atcgtcgtcg gtccagtcat catggttatc gggttgggac ttgcggcaaa tgcagcaaca
    34681 aatgctatgt ataatcaggg cacgtatgat ttcaagtata ttgctgtagc gctgatcaca
    34741 ttaggattaa caattttctt caacatgtgg ctaagaggtt ttctcggatt gattccgatt
    34801 ttactaggta ttattagcgg atacatcgta gccttgctgt ttggaatcgt ggatactaag
    34861 ccaatcgtag acgctgcctg gtttgctgtt cctaactttg agattccctt tgtacaatat
    34921 gaaccaaagc tatatcttgg agcaatcaca acaatggcac ccatcgcttt tgtgacgatg
    34981 acagagcata tcggccatct tatggtattg aataaactaa ctaaacgtaa tttctttgaa
    35041 gaaccgggtc ttcataagac gttgacaggt gatggattag cacaaatcgt ggcagccttt
    35101 gtcggaggtc ctcctgtaac aagttacggt gaaaacattg gtgttctggc tatcacacgt
    35161 gtccatagcg tgtttgtgat cggtggagct gcagtatttg cagtcatttt aggattcgta
    35221 ggaaaattaa gcgctttgat tttaagtatc cctggaccag tcatctcagg aatcagtttt
    35281 atcttatttg gagtaattgc cgcaagtgga atgaagattt taatcgagaa caaaatcgat
    35341 tttgataaaa agaaaaatct gttgattgct tcagttatct tagtagtagg aattggcgga
    35401 ctcgtctttg aagtaggcac atttacattg tcggcaatgg cattagcaac tgtacttggc
    35461 atcattttaa atcttatctt accagaaact tcacgaagcg aagaaaacta aggaggaaac
    35521 attatgatca tcaaatcaga acgtatcagc ttaaagcatt tattgaccgt tgaggcactt
    35581 actgatcaag aagttatggg cttgatccgc agagggcagg aattcaagca aggtgctaat
    35641 tggactccgc aaaagcgcca gtactttgct acaaacctct ttttcgaaaa tagtactcgc
    35701 acacataaaa gcttcgatgt agcggagaaa aaactagggc tagaagtgat tgaatttgaa
    35761 gcaagcacaa gctctgtcca aaaaggggaa acactatacg atacagtact gacgatgtca
    35821 gcattaggcg tagacgtagc ggttatccgt catggtgatg agaactatta tgacgaactg
    35881 atacaaagta aaacaatcca gtgttcgatt atcaatggag gagacggaag cggacagcat
    35941 ccaacccaat gcttgcttga tttgatgacc atttatgaag aatttggtca ttttgaaggt
    36001 ctgaaagttg ccatcgtagg tgatatcact cactctcgcg tagctaaatc caatatgcag
    36061 atgttaaaac gtctaggcgc acaagtattc ttctcaggtc ctagagaatg gtacgacgat
    36121 gaattcgaag tgtacggaca ttatatgcca ttggatgaac tactagatca agttgatgtc
    36181 atgatgatgc ttcgggtgca acacgaacgc catgatggaa aagaaagttt ctcaaaagaa
    36241 ggctaccatc aggaacatgg gttaacagtt gagcgtgcga agaagttgca aaaacatgca
    36301 atcattatgc acccagcacc agttaaccgt gatgtagaat tagctgattc attagtggaa
    36361 ggattacagt cgcggatcgt tcagcaaatg agcaatggag tattcgtcag aatggcaatc
    36421 ttagaagcag tgctaaatgg aaaagcgtaa ttataggagg atgaaaaaat gaaaacactc
    36481 attaaaaacg gtcagatcaa tacacgaaaa aatatgacga caccagcaga aatttggata
    36541 gaagatgggc gaatcaaagc aatcggtaca ggattctcag aagccgaatt tgatgaagtg
    36601 tttgatgcaa agggacaatt gatcacacct ggtttagtgg acgtccatgt tcatttgaga
    36661 gagccaggat ttacatacaa ggagacgatc gaagctggga ctcgctcagc tgctcgtggc
    36721 ggtttcacta ctgtatgcgc gatgccgaat ttaaatcctg tacctgatac ggcagaaaag
    36781 ctgagacaag tatatgacat cattagaaaa gatgcagttg tgaaggtatt gcagtatgcg
    36841 ccaatcacag aaaatctgcg aagtgagaaa ttagttgatc aagaagcact gattgaagaa
    36901 ggtgcttttg ctttcacaaa tgacggtgta ggtgtccaaa cagctgggac gatgtatctg
    36961 gctatgaaag aagctgcaaa aaacaataaa gccctagtag cccatacaga agatgaatct
    37021 ttattatttg gcggcgttat gcacgctggc aagaaggcag aagaacttgg tcttcccggg
    37081 atcttaagcg taaccgagtc ttctcaaatt gctagagatc tgttgctagc tgaagcaact
    37141 ggtgttcatt accatgtttg ccatgtttct actaaagaaa gtgtccgtgt aatcagagac
    37201 gcaaaaaaag caggaatcca cgtaacagca gaagtttcac cccatcatct tattttgatc
    37261 gacgaagata ttcccgaaga tttcggcttt tggaaaatga acccgccgct aagaggacgg
    37321 gaggacagag aagctttgat cgaaggcttg cttgacggta cgatcgattg catcgctaca
    37381 gaccatgccc cccatggatt agaagaaaaa agccaaagtt tcatgaaatc accatttggt
    37441 atcgtaggaa gcgaaacagc gtttcaactg atctatactc attttgtaga aactggtcga
    37501 ttcacgttgg aacaagtgat caattggttg gcagttaaac cggctgagat ttttggtttg
    37561 aatgcaggaa cgctaacggt tggtgccccc gctgatgtag cagtttttga tattacacaa
    37621 acttgcacga tcgataaaga agacttttta tcaaaagggg agaatacacc ttttatcggc
    37681 tggaaagtaa aaggcgaaac acaaatgact tttgtcaacg gaaaacttgt gtggcagaag
    37741 ggagaataaa agtatggaac gtttgcttat attagaagat ggaacagttt tcgaaggaaa
    37801 ggcttttggt gcagatgcta gtgtcttcgg agaaatcgtt tttacaacaa gtatgactgg
    37861 ttatcaagaa actatcactg atcaaagttt taacggtcaa atcattacat ttacctatcc
    37921 tatggtgggt aattatgggg taaatagaga tgattatgaa tcgatttctc caacatgcaa
    37981 aggagtagta gtaaaagaac atgcacgtgt agcttcaaac tggcgaaacc aaatgacgct
    38041 agattcattt ttgaaacaaa aaggaattcc tggaatatca ggtatcgata cgcgcgccct
    38101 tactcgcaaa atccgtgaag tcggaacgat gaaagctagt atagttgatg ctggagattc
    38161 cttcgaacat gcatttgatc agctgaaagc aaccgttcta gcaaccaatc aagtgcagca
    38221 agtctcaact aacaaacctt atccaagtcc aggaacaggt cggaaagtca ttgtcattga
    38281 cttcggcttg aaacatagta ttttacgaga attgagtaaa agaaactgta acctcacagt
    38341 acttccctat aacacagatg ccgagacgat tttagcgcta tcaccagacg gagtcatgct
    38401 gacgaatgga cctggtgatc caaaagatgt gccagaagca atcgaaatga tcaaaaaaat
    38461 ccaaggaaaa gtaccgattt ttggtatttg tctcggtcac cagttatttg ctttagccaa
    38521 cggtgcagat acctataaaa tgaaattcgg acatcgagga ttaaatcacc cggtacgaga
    38581 agtagcgacc ggaagaattg attttacttc tcaaaatcat ggctatgcag tcgatgaagc
    38641 cacgattgat ccagaaaaac tcatcgtaac tcatgtagaa gtaaatgacg gaacagtaga
    38701 aggagtcaaa cacagagatt atcccgcttt cagtgtgcag tttcatccag atgctgcccc
    38761 tggtccgcat gatgcactac atttatttga tgaatttatg gaaatgatgg acgcaggaaa
    38821 ggagcagtaa acagatgccg aaacgtacag atatcaagaa aattatggtg attggatctg
    38881 gtccgatcat cattggtcaa gccgcagaat ttgactacgc aggaacacaa gcctgccttg
    38941 ccttaaaaga agaaggatat gaagtggttt tagtaaattc taatcctgcg acgatcatga
    39001 cggataaaga agtggcagat caagtatata tcgaaccgat cacacttgaa tttgtatcaa
    39061 ggattcttcg aaaagagcgt ccagatgctc tccttccaac tttaggcgga cagacaggcc
    39121 tcaatatggc aatggagctg gcagcatcag gtattttaga agaattgaac atagaacttc
    39181 taggaacaaa gctttctgcg atcgatcaag cagaagatcg tgatctattc aaacagttga
    39241 tgaaagaatt gaatcagcca atccccgagt ctgaaatcgt caatacagta gaagaagctg
    39301 tagattttgc aaataaaatc ggctatcctg tcatcgttcg tccagccttt actttaggcg
    39361 gaactggtgg aggtatgtgt gaaaatgaag aagaattacg tatcgtatca gaaaatggat
    39421 taagtctttc ccccgctacc caatgtttga ttgaacgaag tattgcaggt tttaaagaaa
    39481 ttgaatatga agtgatgaga gattcggctg ataatgcaat cgttgtctgc aatatggaaa
    39541 attttgaccc ggtgggtatc catacaggtg attctatcgt atttgcccct agtcaaacat
    39601 tgtctgacca tgaatatcaa atgcttcgtg atgcttctct ttcgattatt cgagcactta
    39661 agatcgaggg aggctgtaat gtccaactcg ctcttgatcc acatagtttt aattattatg
    39721 tgattgaagt caatccgcgt gtttctcgtt catctgcgtt agcaagtaag gcgactggct
    39781 atccgatcgc taagctagct gctaaaatag cgcttggatt aacattagac gaaatgaaaa
    39841 atcctgttac aggaacgact tttgcagaat ttgaacctgc attagattat gttgtagcta
    39901 aaattcctcg ctggccgttt gataagtttg aaaacggaga acggctttta ggcacgcaga
    39961 tgaaagcaac tggtgaagtg atggctatcg gtcggaatat cgaagagtct ttattgaaag
    40021 ctgtgcgttc tttagaaatc ggtacacacc atatcgaact tcctgagttg gcagacatca
    40081 cggacgatga cctaatccaa aaagtggttc gagcacaaga tgatcgcctg ttttatgtag
    40141 ctgaagctat tagaagaggg tacaccatag aagaattagc ggatttgaca aaaatcgatt
    40201 tattcttttt agataaactg ttgcatatcg tagaaatcga acagcagttg aaacagcagc
    40261 cgttggatga caagatattg aaaacagcga aacaaaacgg ttttacagac aaaaaagtgg
    40321 cggagctgtg ggaaataaac gaagaagccg ttcgccagat gagaaaagaa aatgatttga
    40381 ttcctgtata taagatggtt gacacttgtg cagcggaatt cgaatcaaaa acaccttatt
    40441 tttatagttc ctatgaatat gaaaatgaaa gttcacgatc agaaaagccc tcaattctag
    40501 tactgggatc tggtcctatc cgtatcggac aaggggttga atttgactat gcgactgtgc
    40561 attctgtcaa agcaatccag caggcaggat acgaagcaat catcatgaac agcaatcctg
    40621 aaacagtttc gactgacttt tctatttcgg ataaattata ttttgagcca ttgaccttcg
    40681 aagatgtgat gaatgtgatc gagctagaac agccgatagg agtaatcgtt caatttggtg
    40741 gtcagacagc aatcaactta gccgaacctt tgactgctgc tggtgtaaaa atcttaggga
    40801 caacgatcga agacttggat cgtgcggaaa atcgtgattt attcgagcaa gcgctgaaag
    40861 agttggatat tcctcaacct ttaggggata ctgctacaag taaagaagaa gccgtggtta
    40921 tcgcttcgag aattggttat ccagttcttg ttcgtccaag ctatgtatta ggcggacgtg
    40981 cgatggaaat cgttgaaaac cagcgagatt tagaagatta catggaacat gctgtgaaag
    41041 catctccaga acatccagta ctagtggacc gttacttgct tggtggtgaa tgcgaagtgg
    41101 atgcgatttg tgacggtgaa acagtactca tcccagggat catggagcat attgagcgtg
    41161 ctggtgtcca ttcaggggat tcaatggctg tgtatccacc tcaaacatta tctgaatcga
    41221 tcaagaaaac gatcgaagat tacacgatcc gcttagcatt aggtttgaat tgtgtcggga
    41281 tgatgaatat ccagtttgtc atccatgaaa atgaggttta tgtgatcgaa gtcaatcctc
    41341 gagcaagtcg gactgttcca ttcttaagta aggtgacagg aattccaatg gcacaaattg
    41401 cgacgaaagc tattttggga gagaaactaa agacacttgg ttatgaaaac ggactttatc
    41461 cagaaacaaa agaagttcat gttaaagcac ctgtcttttc ttttacgaaa ttgcagaaag
    41521 ttgatactta cttagggcct gaaatgaaat cgactggaga agtaatggga tctgacagac
    41581 aattagacaa agctttgtac aaggcattcg aagcttctgg attacacttg cctgaatatg
    41641 gagccgtatt atttacgatt gcggacgaaa caaaagaaga agcactagaa ttagctaaac
    41701 gttttacaga aattggctat agtctgatag gaacgaaaca tacagctgct tatttcgaaa
    41761 aaaatggtct ggtcgttgaa acagtagcga aaatttctga ggaagcagca gaaaaaaatg
    41821 tagtcgactt aataagagat ggtaaaacac aagtggttgt caatacgatt gataaagatc
    41881 ggggaaatgc ttctaaagac ggtttcatca tccgaagaga agcagttgaa catggtattc
    41941 cattgtttac ttctttagac acagcagatg cgatcgttcg agtgatggaa tcccgtgctt
    42001 tttcaactaa ccctatttaa cggcgagcac atgttaagac aaaaagacga aaaaccgttg
    42061 caggagggga cagatgaaac aagaaatgat ggaaatcgtt cagcaaaaac agctggcacc
    42121 aaagattttt gaaatgacat tgaaaggtga tctagtaaaa gaaatgcagc aaccaggaca
    42181 gttcttgcat attctcgtgc cgcgtgcaga cttgctatta cgccgaccga ttagcttgaa
    42241 tcatatcgac cacgaaacaa atacttgccg tatcatttat cgggtggaag gtgaggggac
    42301 tagagtcttc tctactcttt ctgccggaga ccatttagat gtcatgggtc ctttaggcaa
    42361 tggttttgat ttgtccaatc ttcatacagg agacgaagta tttataatcg gtggaggaat
    42421 cggtgtccct cctctatatg agttatcaaa acagttagtc gaaaaaggaa ttaagccaac
    42481 gcatttttta ggttttgcca cacatgaagt cgtttattat gaaaaagaat ttaatcgtct
    42541 aggagaaaca agaatcgcta ctgacgatgg aacatacggt atgcacggaa atgtcggtaa
    42601 tttattgtta ggaacgaaga cggaacctac agctgttttt gcatgtggtt caaacgggtt
    42661 attgaagaca gtggaacagc tgttttcttc gcatcaaaat gtccagttat cattagaatc
    42721 ccgcatggca tgtgggatag gggcttgcta tgcttgcgtt tgtcatacgc cggaagatga
    42781 aacaggaaca aaaagtgtga aagtttgtga cgaaggaccg atttttaaaa taggagaggt
    42841 aattatatga atgagaatcc tttagcggtc aaacttcctg gcctagaatt aaaaaatcct
    42901 atcatgccgg caagcggctg tttcggtttt ggcaaagaat acggaaaata ctatgatttg
    42961 aatcagcttg gatcgatcat ggtcaaggca acgactccaa atgcccgctt tggcaatccc
    43021 acgccaagag tggcggaaac accatctggc atgttgaatg caattggttt gcaaaatcct
    43081 ggtctagacg ttgttatgca taaacttctt ccagaattag aggagtacaa tgagttaccc
    43141 atcatcgcaa atgttgctgg tgcttgtgaa gaagactatg tagaagtttg tagcaaaatt
    43201 ggcgaagcac caaatgtgaa agcaatcgag ctgaacattt cttgcccaaa tgtcaaacat
    43261 ggcggaattg cttttggaac agattctgat gttgcttttc aattaacaca agcagtaaag
    43321 aaagtttctt ctgttcctgt ctatgtcaaa ttgtccccaa atgtaacaga catcgttcca
    43381 atcgctcagg caatcgaagc tggaggggca gatgggttta cgatgatcaa tactcttctt
    43441 ggtatgcgga ttgaccttaa gacaagaaaa ccgattttag ccaatcaaac aggtggactc
    43501 tccggaccag caatcaagcc tgtcgctatt cgtttgattc accaagtagc aagtatatcc
    43561 agccttccaa tcattgggat gggcggcgtg cagacagtgg atgatgttct tgagatgttt
    43621 atggctggag caagtgctgt agctataggg accgcgaatt tcaccgatcc gtatatctgt
    43681 cctaaactaa ttaaagagct ccctgtgcga atgagcgaac taggcatcga atcgctggaa
    43741 agattaatca aagaagtgag agaggaacgt gaacgatgaa tcaactagac caacgaccaa
    43801 ttattgcatt agatttttca actaggcaag aggtagaaga ctttcttcga tttttcccga
    43861 aagaagaaaa attgtttgtg aaaatcggaa tggaattgtt ctaccaagaa ggacctgaaa
    43921 tcgtacgtta tctaaaagag gctggtcata atgtattttt agatctgaaa cttcacgata
    43981 ttccgaatac ggtcgaaaaa gcaatgcggg gattggcaaa gcttggtgtt gatctcacct
    44041 gtgttcatgc cgctggcggt attcgtatga tggaggcggc aatgcgagga ttagaagagg
    44101 ggactccaga aggtggaaaa cgtccgctac ttctagctat cactcagctt acttctacaa
    44161 gtgaagaaga aatgcatgcc gatcaactaa tcgaagtttc tttagaaaaa agtgtgatcc
    44221 attatgctag ctgtgccaaa aaagcaggat tagatggtgt ggtttcttct gcttgggaag
    44281 tagaagcgat caaagaaatg gcaggagacg aatttgtttg cttaactccg ggtataagac
    44341 cagaaggaac agtcgcagga gatcagacaa gagtagtcac accttctcag gcaaaaaaga
    44401 tcggctcaac ttttatcgtt ataggtcgac caatcactca gtcaactaat ccttataaag
    44461 catatcaaac gattcaaaca gaatggagct aacctaaaat gaatgttgaa caaagtatcg
    44521 caaaagactt attagaaatt gaagcagtat ttttaaatcc aagtaatcct tttacatggg
    44581 caagtggaat caaaagccca atttactgtg ataaccgaat cacgatgagc tatccaaaag
    44641 tccgaaaaga gatcgcaaaa ggattagcaa gcaaaatcaa agaagcttac cctgaagtac
    44701 aagtgattgc tggaactgca acagcaggaa tccctcatgc cgcatgggta gcggaaatac
    44761 tggatttgcc aatggtctat attcgaagta aggcgaaaga tcatgggaaa ggtaaccaaa
    44821 tcgaaggacg tatatttgaa ggtcagaaga tggttgtgat cgaggatttg atttctacag
    44881 gcggaagtgt cttggaagca gctgaagcag caaaaagaga aggtgcggat atccttggtg
    44941 ttgcggctat ctttacgtat gagttgccaa aaggaaaagc aaatttcgaa aaagccgaaa
    45001 tccctcttat gacgttgacc aattattctg tattgattga agcagcttta gaagatcgtt
    45061 atatcgatga gcaagaatta actctgctaa aagaatggaa aaaagatcct gaaaattggc
    45121 agattgaagc gcgcttgatg aagatagaga aatccgattt gtgtctccag aatcatcaat
    45181 tggaatagca aaaatccaaa aactgattgc gagaagcatc ttttcatcag gtatgaaaag
    45241 aaacttctat cgtgatcagt ttttgatttt tttaaaattc atttttctaa gactggttag
    45301 cagaaagaag agaataatct cttctctttt ttctattata tatagatttt ctctattttt
    45361 tggtaaggga gctgtttcaa aaatggtaaa gagcggaaat gttgcgaata tcagttattt
    45421 aatgatatga tattcggaaa aagaaatctc aaagggaggt aacttttatg gggttgaatg
    45481 aatttatttt aggattgaac aatttagaga cgatcacaag ggcacctgga ttttttaaat
    45541 atacagagca tacagttgca gcacattcgt atcgtgtagc atctattgcg caagttctag
    45601 gagacattga agaagcgaat ggacaaccga tcgattggaa actgctctat gaaaaatcat
    45661 tgaatcatga ctacacagag cgttttattg gtgatatcaa aacgccggtg aaatacgcaa
    45721 gcaaagagtt aagagggatg ctgcaaacag ttgaagaaaa gatgacggac gaattcatca
    45781 accaagagat tccagctgag ttccaagaag tttatcgccg tcggttgttc gaaggaaaag
    45841 atgaaaccgt agaaggagaa ctattagcca tttcagataa ggttgacctt ctttatgaat
    45901 cttttgatga aatcatcaaa aataacccgg aaaaagttta taaggagatg ttcattgagg
    45961 cagtgactac gattaaaagc tttgaacatc ggccttctgt tcagtatttc tttaaagaga
    46021 tatttccgga actattgaac caggaatttt atggaaaaga ggcgttttta gaaaccgtct
    46081 caacgattgt aaagaaagaa gaaaaataaa aatgatatga gatgcagggg gaagtatagt
    46141 tgaaaaagaa tatggatgtc gaatggggct attctggaga caaaggtccg gaaaattggg
    46201 cgagtttatg tgattggttt gcgagaggtg cggaatttcc attgcagtcg cctattcatt
    46261 tgaccaaggg ggaagaaacc aaaatagaag gagacacgct ttcctttcat tatcaaaagc
    46321 aacggtttac tgacaaggaa ttcaaaaaca cgattcattt agtacctttt gaccaactca
    46381 gttatgtaga gttcaagaaa gaacgctatt atttgacaga tattcatttc cacttgcctt
    46441 ctgaacatat catcaacgga aaacaggaag atattgaatt tcattttgtt catatgaaca
    46501 gcaaaaaaga gaatctagtt gtgggtgtca tgtaccagct aatggtacag gaaggttggc
    46561 tgtataatca agaaaatggg gaaagttgga acttagaaaa acatgagcat tgggtaaatc
    46621 cggatgtgtt ctttccggag caaaaaagcc atttccatta tattggctca ttgacaactc
    46681 cgcctaccag cgggccaatc caatggtttg taatggatca tgtaggaaaa ctgaaccaag
    46741 agtttcttct ccccttcgat ggtcagtatg ctcgaccgaa caaccgacca atccagccct
    46801 tgaatggaag agaaatttat tatttcgagg agtttgagca atgaatattc agcagatgaa
    46861 atacgtggtc gctgtagcaa acaatggtag tttcagagaa gcggcaaaga aaatgtttgt
    46921 ctcccagcca agtctttcag ctggtatcaa agaattagaa accgagttag ggatcacgct
    46981 gtttactcga acgaatcgag gggcatattt aacagaagaa gggcaagact tcttaaatcg
    47041 tgccgagaaa atattggtcc agcttgatac attagaaact cactatctaa cgagtgaaaa
    47101 gagagacaat ttttcgatcg ctgcccagca ttatgatttc ttaggtcctt tgaccgcaca
    47161 gctactcgag gaattcaaag acgagatcaa acaatttcgg atcatggaga cgacaacagc
    47221 gagagtgatc gaagaagtca aagagcagca cagtgagatc ggcattattt atatgaatga
    47281 gcataatcaa gctggtatca gaagatattt acaacaagga gagctaagtg gccgcagcat
    47341 tggaaaattc aaaacgcata tttttttaag gcaaggacat ccattagctg aacacgcaga
    47401 gatcaccaaa gaagaattgt tatcttatcc tcaagttcgt tttacacagg atggaaataa
    47461 tttcccgtat ttttatgaag accttatcga gatccctgat caagaaacag tgatttatac
    47521 gagtgatcga ggtactttga tgaatatcgt tcttgaaact gatgcttacg cttcaggatc
    47581 tggtattgtg attggaggga ttcgcgagca cttgcggtta atccctttgg cagaaggcga
    47641 actaaatgaa ttttatatca tttattcggc gaaacgtccg ctcagtgaaa tcgctcaaag
    47701 atttatcaaa gagcttactc ggttactgga ccagcagagc taagaaaagc agatgagaac
    47761 gctgtttatt gaacaccgtt gatcaaaaaa gaggggttgt gacagaattt tgtcacaact
    47821 cctctttttg tagcaaacaa tttataacta attgctgttg tgggaaagta cgcaaatatg
    47881 attctagaaa cactcattga ttacctgttt tgtttcatag ctgtaatttt ttcttcttct
    47941 ggtgtcacga taatcgtctt ttggttttca taaataacat aaccaggctt ggcgccattt
    48001 ggttttcgga tatgctttac ctgtacaaga tcaaccggca cttgggcaga gtatcggtat
    48061 tttgaaaaat aggcagctaa ctcggcagct tctgtaattg tttcatctga gggcttgtca
    48121 cttttaatga tgacgtgcga accgggtata ttttttgcat gaagccaata gtcggttttt
    48181 ttagctgtct tcattgtcag ctggtcattt tgaagattgt ttttaccaac aagaatgagc
    48241 gttccgtcag aagaaaagta ttgatcaggc tgagatggtt tcttacgttt ttgttttttt
    48301 aagttcttct ttttcaggta tccttctgaa gtcaattctt ctttaattgc ttcgatatcc
    48361 attgggccgg caatttccag ttgagataag acagattcaa gatattggat ttcatctttg
    48421 gcttcttgga tttgttcgcc aatcagctta acagcgttct tcagcttttg gtatcgatgg
    48481 aaatatttct gtgcattttg atttggtgtc aatgccggat ctagtgctat tttgattggc
    48541 cgatcttctt cataataatt aggtaaaaca acttcttttg cgccgcgtgg tacttgtgtc
    48601 agaaaagtag taagtagttc gccatttcta cggtaattct cagcgttttc ggattctttg
    48661 agtgtttgtt cacgtttttt caatttattt ttatttcgtt ttagttcatt ttcgattttt
    48721 cgaatcagtt ctccgccttg ttgttttgcc cgatctttct ctgctttttc gtgataatag
    48781 ctatctaata attgactgag tgttggataa gctgttacag cagatgcctg ttcggacaat
    48841 acctgataag caatcggtgt gaaaaaatct ttattttccg tttcgtaaaa agtaggaata
    48901 ggctgatggc tgatggctga gaaaaattcg tcccatacaa ccatcttttc attaggacgt
    48961 tccagtaatc ttttggtcag ctcttgagcg atatcgaaac cgattccctg aaactgacgt
    49021 tggattgctt ttggatctag ttcagtttgg gaaagccgct gaaagatttt ttctttctcg
    49081 gagctaaatg ggttcaagac ctcttgtttt ggcggagcaa catattctac accaggaagc
    49141 aaagaacgat aagtgttttg agagcttcca atgtgtttga tcgcatccag tatttttcca
    49201 gtttctcgat taactaagac gatcgtacta tgacgtccca ttaattcaac gatcaatatg
    49261 atattttgta gatcacccaa ctcgtttcgt ttagcaaagt ggaaatggat cactcgatca
    49321 ttttcgattt gttcgatgtc ttctaaaata gcgccatcca aatattttcg aagcatcatc
    49381 acgaagttag gcggattatc aggattctga taatcgattt gggtaatctg tacacgagca
    49441 taacttgggt gcgcagataa taataatcga tggtttttgc cgcgtgaacg aatcaccagc
    49501 acgatttcat tttcataagg ctgatggatt ttcgagatcc ggccagatag taacgtttca
    49561 cgcaattcgt tgaccatcgc gtgggtaaat acaccatcaa aggacatggt tcattcctca
    49621 tttcttttca taagtatatt cattataacg aaaaaaagaa gaaaaaccaa tttggccgaa
    49681 tagtaaagca aaagtctaga aaaatcttgg actaattaca taaaaaacag tgtttctatc
    49741 gttgaaatcc aaatttttag gta
//
LOCUS       DAYYJC010000009        49210 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000009 DAYYJC010000000
VERSION     DAYYJC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.009"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..527)
                     /locus_tag="ACHA9N_000498"
     CDS             complement(<1..527)
                     /locus_tag="ACHA9N_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9083990.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYF"
     gene            complement(583..3063)
                     /locus_tag="ACHA9N_000499"
     CDS             complement(583..3063)
                     /locus_tag="ACHA9N_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083991.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(3047..3382)
                     /locus_tag="ACHA9N_000500"
     CDS             complement(3047..3382)
                     /locus_tag="ACHA9N_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9083992.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(3396..4430)
                     /locus_tag="ACHA9N_000501"
     CDS             complement(3396..4430)
                     /locus_tag="ACHA9N_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9083993.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(4520..5200)
                     /locus_tag="ACHA9N_000502"
     CDS             complement(4520..5200)
                     /locus_tag="ACHA9N_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9083994.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(5484..6587)
                     /locus_tag="ACHA9N_000503"
     CDS             complement(5484..6587)
                     /locus_tag="ACHA9N_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9083995.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(7216..7686)
                     /locus_tag="ACHA9N_000504"
     CDS             complement(7216..7686)
                     /locus_tag="ACHA9N_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9083996.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            8016..9656
                     /locus_tag="ACHA9N_000505"
     CDS             8016..9656
                     /locus_tag="ACHA9N_000505"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9083997.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            9810..11132
                     /locus_tag="ACHA9N_000506"
     CDS             9810..11132
                     /locus_tag="ACHA9N_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9083998.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(11200..11634)
                     /locus_tag="ACHA9N_000507"
     CDS             complement(11200..11634)
                     /locus_tag="ACHA9N_000507"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9083999.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(11699..12536)
                     /locus_tag="ACHA9N_000508"
                     /pseudo
     CDS             complement(11699..12536)
                     /locus_tag="ACHA9N_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(13278..13904)
                     /locus_tag="ACHA9N_000509"
     CDS             complement(13278..13904)
                     /locus_tag="ACHA9N_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084000.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(14199..14534)
                     /locus_tag="ACHA9N_000510"
     CDS             complement(14199..14534)
                     /locus_tag="ACHA9N_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9084001.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(14521..14805)
                     /locus_tag="ACHA9N_000511"
     CDS             complement(14521..14805)
                     /locus_tag="ACHA9N_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9084002.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(14861..16384)
                     /locus_tag="ACHA9N_000512"
     CDS             complement(14861..16384)
                     /locus_tag="ACHA9N_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9084003.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(16377..17552)
                     /locus_tag="ACHA9N_000513"
     CDS             complement(16377..17552)
                     /locus_tag="ACHA9N_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9084004.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(17556..17741)
                     /locus_tag="ACHA9N_000514"
     CDS             complement(17556..17741)
                     /locus_tag="ACHA9N_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084005.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(17707..19401)
                     /locus_tag="ACHA9N_000515"
     CDS             complement(17707..19401)
                     /locus_tag="ACHA9N_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9084006.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(19398..19871)
                     /locus_tag="ACHA9N_000516"
     CDS             complement(19398..19871)
                     /locus_tag="ACHA9N_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9084007.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(19940..20095)
                     /locus_tag="ACHA9N_000517"
     CDS             complement(19940..20095)
                     /locus_tag="ACHA9N_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9084008.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(20229..20609)
                     /locus_tag="ACHA9N_000518"
     CDS             complement(20229..20609)
                     /locus_tag="ACHA9N_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9084009.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(20613..20828)
                     /locus_tag="ACHA9N_000519"
     CDS             complement(20613..20828)
                     /locus_tag="ACHA9N_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084010.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(20831..21238)
                     /locus_tag="ACHA9N_000520"
     CDS             complement(20831..21238)
                     /locus_tag="ACHA9N_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9084011.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(21504..22979)
                     /locus_tag="ACHA9N_000521"
     CDS             complement(21504..22979)
                     /locus_tag="ACHA9N_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9084012.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(22969..23817)
                     /locus_tag="ACHA9N_000522"
     CDS             complement(22969..23817)
                     /locus_tag="ACHA9N_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9084013.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(23854..24216)
                     /locus_tag="ACHA9N_000523"
     CDS             complement(23854..24216)
                     /locus_tag="ACHA9N_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084014.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(24217..24375)
                     /locus_tag="ACHA9N_000524"
     CDS             complement(24217..24375)
                     /locus_tag="ACHA9N_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084015.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(24516..25140)
                     /locus_tag="ACHA9N_000525"
                     /pseudo
     CDS             complement(24516..25140)
                     /locus_tag="ACHA9N_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(25137..25448)
                     /locus_tag="ACHA9N_000526"
     CDS             complement(25137..25448)
                     /locus_tag="ACHA9N_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084016.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(25492..25785)
                     /locus_tag="ACHA9N_000527"
     CDS             complement(25492..25785)
                     /locus_tag="ACHA9N_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084017.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            25979..26626
                     /locus_tag="ACHA9N_000528"
     CDS             25979..26626
                     /locus_tag="ACHA9N_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084018.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            26687..27832
                     /locus_tag="ACHA9N_000529"
     CDS             26687..27832
                     /locus_tag="ACHA9N_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084019.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(27913..28218)
                     /locus_tag="ACHA9N_000530"
     CDS             complement(27913..28218)
                     /locus_tag="ACHA9N_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9084020.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(28243..28674)
                     /gene="ruvX"
                     /locus_tag="ACHA9N_000531"
     CDS             complement(28243..28674)
                     /gene="ruvX"
                     /locus_tag="ACHA9N_000531"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9084021.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(28671..28937)
                     /locus_tag="ACHA9N_000532"
     CDS             complement(28671..28937)
                     /locus_tag="ACHA9N_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9084022.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(29192..29917)
                     /locus_tag="ACHA9N_000533"
     CDS             complement(29192..29917)
                     /locus_tag="ACHA9N_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9084023.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(30278..30348)
                     /locus_tag="ACHA9N_000534"
     tRNA            complement(30278..30348)
                     /locus_tag="ACHA9N_000534"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30314..30316),aa:Gly,seq:ccc)
     gene            30465..31217
                     /locus_tag="ACHA9N_000535"
     CDS             30465..31217
                     /locus_tag="ACHA9N_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9084024.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            31233..31712
                     /locus_tag="ACHA9N_000536"
     CDS             31233..31712
                     /locus_tag="ACHA9N_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9084025.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(31963..33543)
                     /locus_tag="ACHA9N_000537"
     CDS             complement(31963..33543)
                     /locus_tag="ACHA9N_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9084026.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(33536..34279)
                     /locus_tag="ACHA9N_000538"
     CDS             complement(33536..34279)
                     /locus_tag="ACHA9N_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084027.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(34455..35231)
                     /locus_tag="ACHA9N_000539"
     CDS             complement(34455..35231)
                     /locus_tag="ACHA9N_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9084028.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(35479..36315)
                     /locus_tag="ACHA9N_000540"
     CDS             complement(35479..36315)
                     /locus_tag="ACHA9N_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9084029.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(36326..37255)
                     /locus_tag="ACHA9N_000541"
     CDS             complement(36326..37255)
                     /locus_tag="ACHA9N_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9084030.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            37447..37800
                     /locus_tag="ACHA9N_000542"
     CDS             37447..37800
                     /locus_tag="ACHA9N_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9084031.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(37874..38530)
                     /locus_tag="ACHA9N_000543"
     CDS             complement(37874..38530)
                     /locus_tag="ACHA9N_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9084032.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            38756..40690
                     /locus_tag="ACHA9N_000544"
     CDS             38756..40690
                     /locus_tag="ACHA9N_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9084033.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(40776..41999)
                     /locus_tag="ACHA9N_000545"
     CDS             complement(40776..41999)
                     /locus_tag="ACHA9N_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9084034.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(41993..43864)
                     /locus_tag="ACHA9N_000546"
     CDS             complement(41993..43864)
                     /locus_tag="ACHA9N_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9084035.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(43885..45003)
                     /locus_tag="ACHA9N_000547"
     CDS             complement(43885..45003)
                     /locus_tag="ACHA9N_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9084036.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(45086..45919)
                     /locus_tag="ACHA9N_000548"
     CDS             complement(45086..45919)
                     /locus_tag="ACHA9N_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084037.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(45912..46829)
                     /locus_tag="ACHA9N_000549"
     CDS             complement(45912..46829)
                     /locus_tag="ACHA9N_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084038.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(47056..48180)
                     /locus_tag="ACHA9N_000550"
     CDS             complement(47056..48180)
                     /locus_tag="ACHA9N_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9084039.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            48363..>49210
                     /locus_tag="ACHA9N_000551"
     CDS             48363..>49210
                     /locus_tag="ACHA9N_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084040.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFS"
BASE COUNT        14726 a         9930 c         8245 g        16309 t
ORIGIN      
        1 tggaaataaa aataacaaat ttgactttct ggaccggtca gccgcccgct ttttattttc
       61 agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa
      121 ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa
      181 agaagtatct gatattttgt agaatagcgc aagtagcaat gataataaac atcgactgct
      241 gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt
      301 ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca
      361 actgataagc gggtaagtac gttattaacg gaaatctctc ctttttcctc taataaaagt
      421 tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc
      481 actcctccaa gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag
      541 gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc
      601 tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct
      661 atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc
      721 tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gttttgatcc
      781 agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa
      841 agaagtcaat gtccctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac
      901 atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt tggggagatc
      961 gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag
     1021 aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt
     1081 aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta cagaagtatc
     1141 tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag
     1201 atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa
     1261 ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc
     1321 ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc
     1381 tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact
     1441 atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc
     1501 ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac
     1561 caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt
     1621 atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc
     1681 taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt
     1741 tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact
     1801 atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt
     1861 taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc
     1921 tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac
     1981 gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa
     2041 acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg
     2101 gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc
     2161 acttgttgag atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc
     2221 tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa
     2281 aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa
     2341 aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact
     2401 cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg
     2461 aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa tatctgccaa
     2521 tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc ctgctgataa
     2581 taaactaccg ctagtaacct tgatgggggc accggcaata tacaagtagg cgttgttcca
     2641 gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga tgatcgccac
     2701 aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta gtttcacatg
     2761 gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac catcgttcaa
     2821 ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa gcttcccgcc
     2881 attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt atggatcata
     2941 agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga taaatatact
     3001 taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa
     3061 catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga agccataatt
     3121 ctataccaat gatcaaataa agaaaacgga tcttttcttc attcgtctcc gggaatacgc
     3181 catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat gcgccattga
     3241 ctgaaccact tggatgcgga actggactta gatcataaaa tgtaatcgta cctgaacttt
     3301 tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca ataatccctc
     3361 ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc ttttttcttt
     3421 ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc agcaaccaat
     3481 cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt ttcatttact
     3541 ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc attactgtcg
     3601 cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc accagctaaa
     3661 tcaagattca agtgaaaatt cgaatttggt gcaactaaaa attcattaga cgtattttca
     3721 atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct gataatccta
     3781 ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc gtccaaatct
     3841 cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa aacgactgcg
     3901 agattataag caatacgatt tttgatttgt gctccttctg tagcattttc tgtttcagct
     3961 tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct ttttgctggg
     4021 attttcactt ctataggtat agttgtttct gcatgtgctg atacattgat ttcctttttc
     4081 gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt tgggtttttt
     4141 tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga gacctcaaat
     4201 gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt ttgttccggt
     4261 gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg aaataccggt
     4321 ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa aatgaaaaat
     4381 agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat tgtccaccac
     4441 atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc accgcagttt
     4501 gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa gtcaaatcag
     4561 ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct ggatcataaa
     4621 cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt gtcgctgcac
     4681 ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg ttatctgacg
     4741 tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt
     4801 ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca tctgcaccta
     4861 cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg tctacttgat
     4921 acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcagga actacatcca
     4981 atgttaaaga acctgtctgg ccagttgctg gattttccgg atctgctggc tctactggtt
     5041 ttgaaggatc agtagggtta agaggctcaa cggtatttgt tggttcagta aaggtaatat
     5101 ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg cctagaagaa
     5161 gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac tcctttttct
     5221 tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc atccgcttac
     5281 ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat
     5341 attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt
     5401 attctattag tgacatcctt gaactataaa aataggagta cggcatcatc tgccgcactc
     5461 ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt tatttttttg
     5521 atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc tgttttaaag
     5581 aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata aataacgggt
     5641 ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg ttgtccacgt
     5701 cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc cacatattgc
     5761 aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt agatacccaa
     5821 attccaccag caccagattc taatgatcga tagacatctt cttctgattg aacacctttc
     5881 acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg actaagtttt
     5941 tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg ataagcctga
     6001 acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc acggtttccg
     6061 ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt ggccacttca
     6121 agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca ttgcggtgct
     6181 ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga acttgctgta
     6241 tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt cacatgagct
     6301 aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc ttcgtcaaaa
     6361 tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat caaacgatga
     6421 ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact gctgatgtaa
     6481 ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc catattgata
     6541 aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc aatgccttct
     6601 ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat attactcttc
     6661 gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt cactactctg
     6721 gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca aaagtagtgt
     6781 caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta ttaagtcaaa
     6841 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc gaaactaagc
     6901 gactttttca caacctctcg ataaaacggt gttcaatcag ctgaccctcg gtattagctc
     6961 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
     7021 acgttgactt cacagtctct tccttaaacg gtgttcaaaa acttgcatct gcggtaataa
     7081 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
     7141 cagggcaggt ttttcacgac ctctgattca cggtgtttct ttttcatcaa catttttacg
     7201 aaatactgtg gaagactatt gaaataagtg tggattgttt ctttgtgttt caatcgtttc
     7261 ttgccattct tcatttaatt ttggattacc gttttcttcc ttgaactcat aaggtcctaa
     7321 ttcaaaaatc ccagacagta tttgattcac gatttcatat agttcagtgt gatcagacac
     7381 atgattcttg aagatcatct cattatcacc acgtttgaac ctcacattcg ggctctcatg
     7441 atgaacctct atgcattttt ctcctttttt taactgacgg acttctgaaa acatctcata
     7501 aatatcttct ccagtaaaac cttcagcaag cgccagactg tccacaagac cgcctactgt
     7561 cagttcagag attattgctt gattggaatc taattttaat gtatagcttg tcgcttctac
     7621 tacgccacat gcattgcaac cgccttctga tagttcgctt tttctaatca attgaacttc
     7681 tttcattttc tcttcctcct cttaattttc tacttatgta tttttatcac ctgacatagg
     7741 tgactcctat caacgctatc atgtgctttt ttcctcagaa atcctattta tccattccat
     7801 ctatccctta ttcttctact tttctttctc tagcatataa gagatacgca tagtattcaa
     7861 gcaaggattg tcgatataaa aagaacaaat actgctattt tgatttcttt tggctatatt
     7921 acatataaat aaaatttatc atacctgctc tttttgacta tatcatcatc tgacttctat
     7981 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggtta aacttattaa
     8041 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatatggaa
     8101 tcactggtat gcttccaagt actgtccaaa cactggaaca acaatctgtt caagcttatg
     8161 gtcaatactt aagcaaacaa acagatttgg aaaaacgtat cttcttaatg aatctattca
     8221 atacgaatcg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     8281 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     8341 cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa catcaaagca agtttgaaga
     8401 acgcggcaga tggacgagac attcgtttga ttgttgtgac tgatgcagaa ggaatcttag
     8461 gtatgggcga ctggggtgtg aacggtgttg acatcgctat cggcaaattg atggtctata
     8521 ctgctgctgc tggtattaat ccagcacaag ttttgcctgt atcgatcgac gctggtacta
     8581 ataatgaaga attattgaat aatcccttat acttaggcaa tcgtcatgct cgtgtagaag
     8641 gcgaaactta ctatgaattc atcgatcaat tcgttcaatc agctaccgaa ttattcccag
     8701 aattgttatt gcattgggaa gattttggac gaggaaatgc tgcagcgatc ttagaaaaat
     8761 acgaagatca aatcactact ttcaacgacg atatccaagg tacaggaatt gttgtcctag
     8821 caggtgtatt aggcggattg aatatatccg gtgaacaatt gaaagatcaa ataattttga
     8881 catttggtgc tggtactgct ggtgtgggta tcgcaaacat ccttttagat gagatgatcc
     8941 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacaaggtt
     9001 tattgtttga agatacagaa ggattgactc ctggtcaaat cccattcgct cgcaaacgct
     9061 cagatttcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcgatccatc
     9121 cgacaatcat gattggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     9181 aaatggctgc tcatacacca cgtccgatca tcatgccttt gtctaatcca acaaaattgg
     9241 cagaagcaaa agcaaaagat ttaatagaat ggacggacgg aaaagcatta gttggaacag
     9301 gtatccctgc tgctgatgtt gaatataatg gtgtaactta ccaaatcggt caagcaaata
     9361 atgctttgat gtacccaggt ttaggacttg gtttgattgc ttcaacagct acacgagtaa
     9421 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     9481 caggagcagc tatcttgcct ccagtagcaa aaatcactga attttctcaa acgattgctg
     9541 aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg tgaagaaatc acggatatca
     9601 aagaagcagt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatc
     9661 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     9721 ggcaaaacag cggccagcca actcctgctc ttttgtcgct tttctttggc ttttttcata
     9781 tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaact gcaactgcag
     9841 ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat caccggcgta agtttgccac
     9901 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttagggaaa ttaccgacga
     9961 atatgattgg accaatcgca gttttggtta ttttcggtaa tttatttcat tttatcggga
    10021 caaaaatccc tatcgtcaaa agttatttgg gtggtggttc cgtttttgcg atcttcgctt
    10081 ctgctgcgat tgctacattt gggatcttac ctgaatatgt agtgaaatcc acagaaaact
    10141 ttgtcagcaa tatgggcttt tgggactttt atattgctgc cttgattact ggtagcatcc
    10201 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
    10261 tttcaatggt cgtctccttt tttgccgtag gactcgtggg catgcttatc ggaaatggtt
    10321 ttgcaaattc cgttttatac atctcattac ctgcgatggc tggtggtgtt ggagccggag
    10381 ccgttccttt atcaacgatt tatgcaaatg ttttaggtac ggatgcttct tctatcattt
    10441 cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt gcagcattag
    10501 ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa tggtaaatta atggaaaaag
    10561 gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc cattccacag ttaggtgttg
    10621 gtttgatggt atcattgact ttcttcatct taggacaaat tggtaactat tttgttccta
    10681 aagtccatgc ttacgcattt atgattatta tcgttgtcat ctgtaaaatc gccaacctat
    10741 taccagagta ttatgaagat gcagctatca tgttcaataa cttgattgtc aaaaacttga
    10801 cagcagctgt tcttgccggt atcggtattg cgttgctgaa tctgaatgta ttagcttctg
    10861 ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt tcagttgttt
    10921 caggatttgt cggtcgtttg tttggtttat atccaattga atcaactatt actgctggct
    10981 tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt cttatctgca gctaatcgga
    11041 tggaacttat tgcttttgct caaatgggca atcgacttgg tggtgccatc gttttgatca
    11101 tctctggttt cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac ctgatcatca
    11161 agtatcggct tggtgatcgg gtattttttt gataaagatt tacaggattt tcaaaaatct
    11221 tttttgcatt ttatcggaat gataaccgat gtgttcataa aattggtgtg cttctactct
    11281 gtattctgca gaattaagcc ttattgtcgt tattcctcgt tttttcgctt cttcttctac
    11341 gccagtcatc aaagcctttc cgatgcctct tttttcagca tatttagaca cagcaagtgc
    11401 cataatattg aacattggat cgctaaaaat cgattcatag gactccgctt gtacatagcc
    11461 agctgctttt ttagaagtag gatcttcata aaccaagaaa aaatttttct ctggctcctt
    11521 cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt atatcatacc ctaattgatc
    11581 cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc atttcccgta acatccaaat
    11641 caccagccaa tttctgattt tttaaaatga atttttttat catagtatac tctttttttc
    11701 atttagaaca atatgtttca gtgattttga cttgcgttag tctagattct cctaatgttt
    11761 gtaagatgac aaaggcattg taacaattga ccaaaagaac cacgaggaca attacttgaa
    11821 aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc
    11881 caataacagc agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac
    11941 ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat
    12001 atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg
    12061 gtccaccacc aatacctaaa aaactagcta aaaagcccag aacccccccc acatataagg
    12121 taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca
    12181 aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca
    12241 aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca
    12301 ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca
    12361 gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca
    12421 gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa
    12481 atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg
    12541 tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag
    12601 taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa
    12661 cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    12721 agctcctcat aattttcgtt tggttgaact tattaccgca gatgcaagct tttgaacacc
    12781 gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
    12841 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac
    12901 accgtttaag gaagagactg tgaagtcaac gttttgacct gattattaga gaaacaaatg
    12961 gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat gccgaggggc agctgattga
    13021 acatcgttta tttggaaaaa gggattgtga caagacgttt gtcacaatcc ctttttttat
    13081 atcttattaa ttcattcttt tgttctattc ttcttcttcc gccatgaatg agaaacccat
    13141 atcaaagaac tataaaggtt atcaaaacag cgtttatgac taaatgaatt agagttatct
    13201 atcactattt ttcaagcgat ttaaaccaaa tggcatacaa tatggcatac aaaaacttca
    13261 ataagccgtg ggggttttta aatttcttct tgttctttaa aatcaatgag tggagctaca
    13321 taatttgaat ccactttttc aaacgtcact ttttgcatcg acgaatgttt tctttcccaa
    13381 gataacgtaa atagatttga ctctggactt ataaagtcta cttcaccttc tagtattact
    13441 ttcttatgca aatattcctc taataaatat agacttgcta aagcatacaa cacattttcc
    13501 aaatttgctt ccttataatt tgaagaacga ccatgtttta ctctgttata tattttccac
    13561 caatctggtg tatcttcagg ccacccatct ccaaaaggtt ttagtgaagt caatgttaaa
    13621 gaattcataa ccctaacacc attatccaaa cacattaatt cgctagattt aataacagct
    13681 ttaaaatcag ttatgctttt attgctactg ctatcaaatc cgcaaagttc tttgaaaata
    13741 atctcacact ccgaaccaat cgctagtagt agtttttgat attcaataga gaatgttttg
    13801 aaattattct catcgagcat cacaaaatct gtggtattaa taaactcatt ttctaaatgt
    13861 agataatact tccaataaaa atttgtaaac tctgctttgt ccattaaatc acctcttttg
    13921 aattataaca aaaaaaacac gtccacaaca gacgtgttct ttgatttcta cacaatcagc
    13981 ttgccaaaac catccaatca gtcctagcat gcctgtatta aaattgaacg ttcatcatct
    14041 atcatctttc ttagtccagc atgtttgcaa ccacacctac aattttcagt ccaacaatag
    14101 atttcgacta gctaagtctt gcaaaattca ggcagctaac ccttcattta ctaccttaat
    14161 tatactaaat acccacaacg tttacaagtg taatcattct aagaaatctg tttagctatg
    14221 atcgttgtat aatctttaaa cgccgttcct tcattgattg aaataatttt ataactcttg
    14281 tttttccaag ctactttcat atcgtttgtt agttcataat cttgatcgta tcgaataata
    14341 aacgtcaacg ttccttcaag aatggtacct acactagcaa gcacgtcttt tagcatttgt
    14401 gaacgcacat tagcccaaca ttcaaataca gtttcttcac tatccaccat ctgcccatct
    14461 tcatctttta cccgttttgt ctttttaaac ttgactctct cagtcaattc atttacattc
    14521 ttaatgattg gcataataag cacctctcat ttgttgtact aatgcttgta ccgttactgg
    14581 tatccgttcg ctgctggctt cttgacggtt caaataccaa tgttgagtaa gtaatgacac
    14641 cgccaaatca aattgctgat aattatccat atctttatcc gttgcgctac tatcaatagc
    14701 actttttata tattcgcctg ctgtatcaat caattgttga ataaagtcat catcaatagt
    14761 gtgatcgatc cgcatacttt tttttatgct atccaaaatt accattttat ctctcctaat
    14821 gaaaaaaagg cagtgggata aaccgccgcc caatattttt ctatactttt ggtgttacaa
    14881 ctggcgtaaa ctcaatatac actgaagcat tttgatcaat tttcttataa tcgttgcgaa
    14941 cgatcactgc aagtccttgt gagtagtaat cgaatttttc ccattgagtc gttacttgat
    15001 ttctacgagc aacaaagata gattgtgcaa tatcccccat aatcataggg aatgttccag
    15061 ctttaggatt ggcaaacaat gtatctgcaa tcaatactac tggcataccg aataatgatt
    15121 taccactagg tgctgtcaca tcgggttgta aaatgtaacg tccatctgaa tctttcaatg
    15181 tatctaggtg gtaatatccg ctttggttta gaattaccat tttatttaat gctgggtcta
    15241 atgccacatt gtatagttgt ttcaaatcgt ccaacgtagc agccgttttc ttagtgaatg
    15301 tttttaataa atccatgata tgcttattgt cagtgttttc taccaatttc gctaactgat
    15361 ctttgacctc ttgtacaata ttcactgctg aatcttccac aacctcattt gataaggcaa
    15421 tcttaccagc acgagtttct actttataat caactgaagt aaacatttca gcgtcaatat
    15481 caccaatttc agcaagttca gcttttgttg ctaacactgc ttgttggtta gttgctactg
    15541 gatatttacc ttgtccattt gatactgttt ttactgttgc atattgagct aagttataat
    15601 ttgaacgttt caaatcaaag acttcaccga ttacttcttc gggaacaact gccgctgcat
    15661 ttacagtagt gactccatca cgcacttcgc cttgtgaacg gatatattct tcatacccac
    15721 gcaattctgt tttttcgtta tcgattaaag ttttttccat gttattactc tccttttgtg
    15781 ctttagaagt ggattctaag ctattgtttg tttgttttgc ttggttgtta ctcataaacg
    15841 attcgtaaga acgcttattt acttggacat ttgatgaatc ataagcggga acggtcacga
    15901 cactaatttc attaagtgct ttgattttat ctattgatcg ttcaattgtg ccatcttctt
    15961 ttttgtcgaa tgaatctaat ccaagcacga acccaaagga catagaatca acaacccctt
    16021 ttgagatatt ctcatacaca tcatttgaat aggtcgtgtc ggttagctct gcttcaaaat
    16081 gtaatcctgt atcatcgaca ttcaatttca acgtacctgc tttgacacta gctagtggct
    16141 ttgaataatc gtgtccatat aataagaaca catttgataa atccacctct tttaatgctt
    16201 cgggtgtaat cacttctaca aagccgccta aatcttttga cggttgcccg aatttcaacg
    16261 catagcctga aatagttttg ccgtctgttg gttgtttttc attttctttc tctactttgg
    16321 gagaatcggc tgttagctca gcttcttctg tcagccgttt ttccttttct tcttcattat
    16381 tcaactggtg taactccttc ctgttctttt aattgatatt tctccaacgt atctaaatac
    16441 gtaaagttca aacttgctag taaacgatcg ccaccatcca tggggggtag ccccattttc
    16501 gaccgcccct cattaatcgt gagaagtgac cctttgacct gatccaatac attttttact
    16561 tttgtttcag gatcagtctc tagtaactga tcggaattaa agcggatatt ggtttcaagt
    16621 ttcgtatcta attcactcac aaacacatta aagtaatgaa tcagcgtact ttggatatac
    16681 tggagattac tttgaacggt gctagaatgt tcattttcaa cgcctaaacg ctcaatcggt
    16741 acaccaaacg ccttggcaat ttgttttgtg ttccaatcat tggaattgat aagcttcaaa
    16801 acatcggtgt ttatttctaa tgttttgtag tccattgttt catcaagaat gatcgttcta
    16861 agagcattat ctccactact tgaaccgttg gcttcttcaa acttttcacg aattgcgttc
    16921 tttgctgatc cgtctaaatc tgacttatga accttcaaaa tacctgaccc actgacacca
    16981 cgagtaaaga aattgtgcaa tgtgcggttg ccagcttgtt gaattttcag ttcatcacgt
    17041 aacgcataaa gtggtggtaa tcctgttaaa ccatcttggg tgaaatattt gaagtgcaaa
    17101 atatcgctag acttcacacg tcttttttta tcaccaatct gataagacaa cgtaccatta
    17161 tctaattgag taaccgttac acttgagtta ggtaataagt gaatttcttc taccttttca
    17221 ccgttgcgtt taatctctgc aaagctatta ccgtttagca acatattgac cgccaaagca
    17281 aactttaaat gccaaccatc catttctgaa ttaggtttct cgtttagtaa cttcaccagt
    17341 tcattatcag cttgtggcat attgtttttg accaattgaa tcggacttga agcaatatca
    17401 ctagcaataa ttcgaacggc tgcaaacaca tcactatttc ttaatgcacg aacgcttgta
    17461 aagctggtgg tgtaatcatc cgattggatt gataccacat gttgtaggaa aggatctccc
    17521 gttgtatttt gtctcgcttt aaaaaatgcc atcttttaac ctcccttctg ttctgtattt
    17581 agcatgaccg ctaccccaat aagcactatg cccagcgcta acaagccata gtagacattt
    17641 gttagtaagg tgatagcaac caacacacac accaatccaa gaagtaaaag aacggtatga
    17701 atgtaattag aaagaaaatt cttcacttgt ataaaattca ttgtctgctt tcgctccttc
    17761 cgttgtggtg aagtaatcca ttgcaaacac ataggcattg attaatgcag ctattggatc
    17821 aattttgtta ctgttctttg atttattaat ctgaatgccg ttgttatccg tttttaaaat
    17881 agcgttgttc actgcatggg tgagaatcgt atttttattg tgaataacgt tgccttcata
    17941 aacttgttct ctaaaagttc gggtcggaac gtttagagta atcgttcctt gtcttacttc
    18001 taacattggg tagttggctt tttcagcttt actgataagc gaattagcgt tatacgggtc
    18061 ataacaaatc cccatacatt ccaaatcatt ttcttgaatc aaatcttgaa taaactgaaa
    18121 tacttggtca tagtccacga ttccactttc taattgagta atggaacact caccagcttt
    18181 ttctagttct cgataaggca aaccgtcgcg cttttctttg tcttgaagcc catatttggt
    18241 ggctacaaag ctatgtgaat cacaataaag ttcgccgtta tctagcggta cgatccatga
    18301 aacgctagtt aaatcatctg ttttagataa atccactccg atataaacgg gcttgcctgt
    18361 aatatcttgt ggttctactt cggttgcctg ccagtcttca gcaatcatat agctgtcctc
    18421 actcgcttgt ctccataaat tgaagttttt caccaataca gaattaaggt tattttgttt
    18481 caaagcaagg ttcacatcat cttgaatggt tggaatcatt acttttttga tttcttcact
    18541 ttcaaaaatc ggattggctt tgatccattt ctcttgatcg tgaatttctt cgggatcgtc
    18601 caattcccat atcgctataa agtacctgtc ggcttgttct tcgcctttta gcacacgatc
    18661 taacaataag tattcctcgt acataggaac gtttaaatca agcccagcgg tactaataac
    18721 acacaataag ccgttctttt gttgggtcat tcctgactta atgacgttgt acgttttacg
    18781 agtttttgct tcgtgccact cgtcaagaat tgcggttgtt ggtgcaaacc catctagcgt
    18841 gctggtttca ctagccagtg ccatagcgaa cgaattagag ggcaagtgct gaatcttaga
    18901 gttcataatt ttcagttgtg gtcttaaaaa cttactagac ttgaccacgt ttctaagtga
    18961 attagacagc atatcatagc ccaatttagc ttgtttcaaa gcgttactta caaacaatac
    19021 ttgccttgct tctgctggtt cttgttccat aatcaacgca ttggcagcca tgccacttgc
    19081 gatatacgtt ttcccattct tacggctcat actggtaaac gctcgattga atcgcctgta
    19141 attcccttct ttagtacgcc agccataaag actgcctaga atgaattttt gaaatcctaa
    19201 catcttgatt gctttaccgt ccgtagatgg tagcaactca atgaatttaa tcgctttggt
    19261 ggcttgttct tcatcgaaca cataagggaa gctatctgac ttagaacgct ccaaatcgct
    19321 tatatgacgc tcacacgctt ggataatttt cttgggtgca atcagcttac ctgataggac
    19381 tttctcaatg taatcattca tgaattcatc aactctttga acgggtcttc gggttcatca
    19441 tttttaacct tgttcaaagc tagttttgat cgtgattcta aggtcatacc tagtgaggtg
    19501 gcttgttttt tcaaatcact catagcttgt gattgaaccc gaaccgctgg attggctttc
    19561 tttactccgt tttcgcatgt gataaacgta cctgattttc ttatctcggc ttcggctgtt
    19621 ttcattcgag caaaggctaa acaataggct accaatgaac cataatctgc ttcactcaat
    19681 ggataatctt tttttaatgt tggtacgata cgattccatt cacttctagc gctcgctgcc
    19741 atccagtcag ggggcgtgat atttattaat ggttgctgct tgaacaattc ttctctagcg
    19801 tccacacgga ttgctttttc ttcacttgat atattgcctt ttgtatcgtg taatatttta
    19861 gggtgtccca tgttgtcctc ctttcagaca aaagaaaaag gaacgtaagc aattaagctc
    19921 acattccttc gtttttcgat cagaattttt ctttatctaa tttatctata taaatgattt
    19981 ttccgttctg catttgaatg gtcacttgac caaaatgagg taactcaatg gtgcgctgtt
    20041 tccctgtttt atcagtaata acaattgtat ttttcaataa atctcctact ttcattatat
    20101 actttttcac ttgtgattac aagtgtaatc gttatttgta attaccctta tgccttttca
    20161 caacggttat ttgtaccaaa atgagtaggc atcgattgtc agtaagtttc acactatgcc
    20221 cccttaactc aaagggtact cttttctcgt ctttgtcttt cattggctgt cttggaatta
    20281 tggtgtgcat ggcataaaga acgaaaatta gtttcatcta accgtctatt ccaatcatct
    20341 tttagttcaa ttttgtgatc tacaacatca gctttcctta tcaagccttc ttctaggcaa
    20401 tcctcacata ctggatgatt taaccgatac aagtatgaag cctttcgcca tgttctactt
    20461 tgatagaatt gaaaatattt accttcgctt tgctttcttt cttcacgctt aattgccgtt
    20521 tgctttgctt tatgcttccc acaatacttc tgcctataat caatcaatgt tttacatcca
    20581 gcatgattac attgtttttt aactgccata gattaccaac caaagcgatc tcgttcgtca
    20641 gaatcatctt gttcccattt tgatttgtat tcaatataag gcgaaactat cttcaagcac
    20701 gttccgtctt taaaattaac tttagtgatt ttatcttcct caaaactaat aaagtctaca
    20761 tcttcttgag tgtttgaata ctcaatgtgt ttctcaccat cgaagtatct aatactttcc
    20821 atatgcataa ttattctacc tccaataaat tagttatcaa ttcatcatct gttagtagca
    20881 aagccaagaa cttatcatgg atgtttaaaa acaaattatc ctcattcgat gtaagctctc
    20941 gttgtctcgc tcgatcatat aggttaagca gacaattaaa ttcatccatt agctcacctg
    21001 tatcatcata gttgccacta tctaccaatg atcccactac tagcgattga caccacgcta
    21061 ctgactcctg cagccaatca ggttcttctt ccattatctt taatacttct ggatcaaatg
    21121 tggaataatc ttctgttacc aatcccacac taaatgccag cgtgttcatt agaaaccgat
    21181 ccatttcatc tgtatattga aagttcggtg ctttgtttgt tgaaataata ttgagcattt
    21241 actttcctcc ttgattcgta acacgtggta acacctacgt aacaggtacc cgttaccact
    21301 caatcccaac ggggacaagg gttctagcgt tttggtaaca ggtaacgggt aaaatccagt
    21361 aacattattc cccagcatat tagctaacta tttcaaaagt tttaaggaaa taaggtgtta
    21421 ccccgttacc aaattgctgt atcccttggg gcagtaaggg taaactagta acgggctagg
    21481 tgttactgac ccgttactac ccgttacttt ttgtactttc cagctcgatt tttattttca
    21541 taggcatttg acttattgta aaatcctcgt cgtttatttc tttttggcag ttgcttccgt
    21601 tcccaaccag gaatactgct aataatcaag gcaatctttt tagtttctgc atctgatcga
    21661 ccattcaata aatcctttgc ttgtttatca aaaacagctt ccaaaatatc agcggtgagt
    21721 acgctatcaa ctaaataaaa ctcttgttgc atagtagctc cattatagaa atgctgtaaa
    21781 tttttctcat tacatttact gtcaggatag ttctgataat aagaacgtct cgcccacgct
    21841 ggagcttctt cccatttata cgggatttcc atttccaaat atttagtgat tgcttcttta
    21901 attggattct ccatcttggc atcctcttga tagtcttttg caatttctaa gacttcttta
    21961 tcttcatcgg gactaaaata gattcgttgt cctttatcaa aaagcacctt tgcttcagct
    22021 aacacttgaa gaaaatattc atcctttgtt ttaaacggat tcatctttgg cttattctta
    22081 caaggcaaag gataaaaacg gcggtttcct gtatcatctt tcaaatactg cccatcattc
    22141 gacgttccaa taaatacaca ttgccttgcc caagctatga cattccgacc ataaggaggt
    22201 ctaattttat cgatcttggc actgatgaag ttttttatct gttcaatttt agttttgttc
    22261 attgaagata attctccaag ttcaataatt acattgcctt gaagctgcat ataatcatct
    22321 ttcttttcac ctagcgaatc taatgaatcg gtgaaaaaat catccgtgta aagtaatgct
    22381 ggaacggtac tcttaccaat cccttgcggt ccatccaaaa ctggaacgat ctcaaacttt
    22441 attcctggtt tatagatacg tgcgactgat ccgactatcc aacgttttgt tacttctcgt
    22501 gtgtaaaggt tgtcgtctac ccctaaaagg tcaataaaca gtgtttccag tctaggctgt
    22561 tgatcccatt taactttttc gatacgttct tttactgggt tgaaaacatt ctttctagcc
    22621 acatttacta ctgctttgtg tattttgttg tcatcaaaaa ctacattgta gttttcttca
    22681 atatatgccg ttaaggcatc ctcaaaactg tcttcccatt ctccagcggt acgattgatc
    22741 caaggagaat cttttaatag gtgaatgtat ccgctaaatt cgttataagc aattgttcca
    22801 gctaaattgc tatcattggt taaaatcaat tctgcattca caacactgtt acttttaatc
    22861 tgatcgtctt tattgcatct taatcgtgac atccaggttt tcaattcaat gatattgtct
    22921 tcacgctcgc tggcttcggg aaagtctaaa atactatcta atttatgctt atgagccatg
    22981 agctttcccc ctcaaatctt ttttaatcat cgatcgaata atttgattca cttctttatc
    23041 ttttagcggt ggttcattgt acgcatttgc tgtataaatc aattctgcta aagtttgatt
    23101 atctacaccg tgccatagta accaaccgca taacttagtt aaatgatcgt ttctactacc
    23161 ttcgccagcg ccttgaacaa tcatatctaa tttttttgct gtccctgttt ttgctacggg
    23221 tcggtattga gtctttattt cttgcggttt ttttagcagt aggttcacta gccaatcggg
    23281 agctggtgca atattattta gagatacgtt ttcaataggt tcatatgctc tcccaacact
    23341 tcttgaaata atcatagttg gcgctattgg aactccttta gtgataatat caatcccgct
    23401 taaactttta cctttatcat cacgcccgaa gatatcagca aatgcacctg tttcttgttg
    23461 gataattaca ccatcaggaa ttttaaagaa gaaatggatt cctttacctg gtgtgatttc
    23521 cgtataagta gaaggaaatg gatcatacat tgaagcaagt tttttataat tctcaacgcc
    23581 gtttcgtgtt tcagaatgtt gatctaggtc aactaccacc atttgatgat cttcaagcat
    23641 taacccaata tttctagttg catcttccgt ccaccattca ttaatttgat tagaatcgtt
    23701 agttgcatca ttccaaccac tagagccttt tattggaatt ttagttccag gagataaggg
    23761 ataaattgac catcccgact tttgtaattc cgttgctgct tgataattat ttgccaataa
    23821 acctctcctc ctttttcaaa atactggtat aaatcattca gacacctcca tgagcttcaa
    23881 ctgctcaagt tcgtatttca acccatcccg aacaaattca gcacctttta attgattctg
    23941 atactttacc catgtattaa atgttttcac ccttgcttct tgcatatcag taatgtttaa
    24001 ccatgcggtt aaaatctgat tttttagctc gttgatttct cgacaaatta ttttgtgttc
    24061 tgcatgtttt ttgagtacat acaaaactgt tagtttttca ttgaatgaat ttccaaagta
    24121 ggttttcccg atattatttt ctagttcagc gatcccttga ttaatttcct ctaattcaag
    24181 gctatcgagt tggtttaata gttttgtttt gtacatctac ttatgccctc cagttcttaa
    24241 ttgatagtcg tactcgtcat aagcagtgaa gagatataac gctaatcctg caaagatagc
    24301 tatttttgca attaatggta ataagccgaa aattccgccg attaaaatgc ttcctataat
    24361 taagtttcgt ttcataatgg ttccctccga ttttttaata tggtataatc ggaacaaaga
    24421 atatcgggtt ttatcccaat taagttgcct ttatcctgtc cgccaagact gttatggata
    24481 aggctttttc tttgttctcg atcatgttaa tgctcctaag ttgtctgcaa ttcagcagaa
    24541 cgattataaa ttttctcact cactgaaaac tgcaacccat atttttcttt aacagtcatt
    24601 aattctactg tctcatctaa aataggttca cgatcaacca acatttcagg agtcatatct
    24661 ttctttttta ccattttgga atgaccataa atagttgata cagctttatt ggctatcaca
    24721 ttcgctttaa tgtagtctac tggttttggt tcagtaatag cgtggcttaa tttagtcatt
    24781 gcttcttttt ggtgttcttt gtctagcatg cggaatactt gaaagccttc aagtcctgtg
    24841 gactggcgta gttctttgat gatgtcgaaa atccagtctt gaaatgcctc ggcttctttt
    24901 ttacggcttt tgaatgccat gcggtaaata ttttttttcg ttgatgatgt ttacatcttg
    24961 tgttctgccc aatgaatcga tgaccttact agtagtaacc ccatctttag gcaaagagtg
    25021 aatggctcta gtaacctgtt taagacctaa cgcttccgca acatctttcg ccactgccca
    25081 ccattcatca ttaatatcga caaatcttat aatgtgtccg ttccaaattt cagttttcat
    25141 tcaacgtcct ccagttcaag ccctagcaag ctcgctaaca actcaacttg ctgatacaaa
    25201 atttcttgat actcttcaat agaaagtcgt tctttaaact ctgcttgtac taaatcgttc
    25261 aagccacgca atactccgct taccgttggt tcttgtggta gcaatttttg atagtccatt
    25321 tgttcgtaca aacattcaaa cgtttcttct agcgctttgc cttccaagtt tgctaattct
    25381 gccgctgtgt cttctgtaag aattgttgat aatacttttt cgattgcttt attttgtgtc
    25441 gattccatta ttatttcctc ctattaatcc ctttgccccc acggcttgcg tttatttttg
    25501 gtattcttcc ataaagcgat cacattcatc ttgtgaaatt cttacgactc catcaactat
    25561 aatcaacttt aaaccgttag gaacatattt ctgcatcata gtattgtatg aaacgttcat
    25621 atacttagca gcttgtgcct tgttcatata ttttggaaac tggtactttt tttctaataa
    25681 tgaaagtaac gttccttgag tttgctctac ttgttgaaca actcgatcgt caataatttt
    25741 aaaagctgga tcatcaagcg tgagtttgtc aatttttaat tccatattta tcccccctcg
    25801 ttagcataca tttctataca aagattttaa aaaaatgcaa gaggctcttg aatacatttc
    25861 tatacctctt ataaactcaa tataccatca taccttttca tttgcaagaa ttttaacata
    25921 cttttttgaa cttttatgtt tcacacatat ttaaatcgtg atatagtaga gaaaatatat
    25981 gaatggtggt gaaaatgtgg ctagaaaaaa ataccccgaa gcagtggaga caggacaaag
    26041 aattagactg gctagaattg aaaagggact ctcaatggaa caacttggag gtaaattatc
    26101 cccaccggcc agtaaaggtg ccgttagtaa ttgggaaaat ggctataatc ttcccaataa
    26161 caccaggctt aaacaacttt caaaggtatt agatgtatcc actacctatc tattaaacgg
    26221 atcttataca ttaagagata tgcatatgat gcccgaaatt aagcgaaatg tgcttgaaaa
    26281 gcaattgaaa aaaactattg aaaaaaataa ctctataatt gattctaatc tttctaatgc
    26341 tatcaataac atcgaagaag ataacctgac tgtctcacag aagatttatt taacaaacgc
    26401 aataaaatat cttaaaacag gatctcgaat tgaatttcct gacgggagta ccccaattgc
    26461 ttatctcgca gctcttctaa atacgctttt acgatatgac tcattaatta atgattcaga
    26521 aaaaaataca gatgagaaaa aagagtatca agaattttat gaaaatgatc ttctaaaaga
    26581 gttcacagct cttgttactg agctaaataa acactattca aaataataat aaactgcctc
    26641 ctccaaccgc ccccacggct tgctacacta tggagggaaa actattatgg caacattcaa
    26701 gcaatatgaa actaaaaaag gtaaattttg gctctatgaa gcctatcttg gaatgaacaa
    26761 aatgactggc aaacctgata aggttcgtag acgaggttac aaaacaaaaa aagaagccca
    26821 gcttgccttg tcacgcttgc aagtggacta cgataaaaac ggtattaaaa aagcgaacaa
    26881 cgaaacgttt aaagaagttt atgacttgtg gctagaaaca tataaaacca ctgtccgaga
    26941 agccaccttc atgaaaactg aaatcaagtt tagaaagtgg attcttccaa aatatggaaa
    27001 tcttcgtgtg aatgaagtca cggtaaaaaa agcccaacaa attgtaaata gctgggctga
    27061 aactactgac caatacaagg tacttcattc aacttctaag cgtattttta gctacgctat
    27121 caatcttggg attattgaga ctaatccact agacaacgta ttaatgccta aaagaaccac
    27181 cactaagcat aaagaaaagg tcaaagttta cagtaaagaa caactgatca ccttgtttga
    27241 ttacctaaac agcaaacctg aaacttatcg aaacgacttt gataaaacgt tactacgctt
    27301 tctcttttat agcggggtta ggatcagtga agcccttgca ttgaattggt cagacattga
    27361 ttttcaagaa aatacggtca ccatcaataa aacactaagc caatccaaaa atggctataa
    27421 aatcagtgat cctaaaactg attgtagtgc tggggttctt ccactagatg aaagaacaat
    27481 ttctatatta aaaaaatggc agattaatca gcggaaatat atgctgacac ttggtgtaac
    27541 tgatccgact atgatttttt gtggtatcta taaagaacta atcacacacc acgctattta
    27601 tgcaagattg attactattt gtgaaaaagc tatggttcca tttctcggtg tgcatgtagc
    27661 aagacacact catgcttcaa tgctcttgga cgctggagct actatggtag aagttcaaac
    27721 tagattacgg catacaaaaa ttagtcagac gatcgattgt tacggtcact tggcaaaaga
    27781 aacgaaagaa aagactgttg aaaaattagt acaacactta aatattgact agatggcata
    27841 caatatggca tacagtaaaa aataaaaaaa ggagaaaccc tatcatatca aggtttctcc
    27901 tagctttata gtctattctt cttcttccgc catgaatgta ttgaatactt cttcaatcat
    27961 gtcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
    28021 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag caggatataa
    28081 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagtagga tttcatataa
    28141 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat ggtcgtggga
    28201 atggttatgt tctgtcataa gattacctct tttcttttat tattaaaata attgcttacc
    28261 tgagccgtct aaataatttt gtaagatcat cacagctgct aatttatcga tgactttttt
    28321 tcgtttggct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
    28381 taatcgttca tcttgataat caactggcaa ggagaatagt tctttgatct tttctccgta
    28441 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atatttttag gtaatccaac
    28501 tacaaaccgg tctacttcat attgtgctac taattccttc aaccgatcaa agccaaactc
    28561 ttcttctgct tcattgatcc gaatgatctc tactccttga gctgtccagc caaaggggtc
    28621 actgatagca acacctactg tcttagaacc gacatccaag cccattactc tcataaattc
    28681 actccatgat ttgagaggta gtccttgaca attacttcca tgatctcatc gcgttcgtga
    28741 cggcgaatca aatttcgagc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
    28801 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
    28861 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
    28921 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
    28981 tacaacctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttccgaa
    29041 taaattaaga aacactattt gtatgttttt ttcctttttt ctgtagcttt atcaaaagta
    29101 ctcttttcat aaagtatacg ctatatttct ttgaatgtat gtttttacac aattttttca
    29161 taatattcat aaaaaattcg aatagctgct actaattttt tgtttttgtt gcttgtcctg
    29221 tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
    29281 ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt tcattcccct
    29341 caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata tatgccgcaa
    29401 tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct gtcatcaagt
    29461 ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg cgattatata
    29521 accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata tacaaaggct
    29581 ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt cctactctgt
    29641 tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta agatcctctt
    29701 ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
    29761 gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta atctgctgaa
    29821 agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc acagcagcga
    29881 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagc
    29941 tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat agaaacagca
    30001 aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga ttcctcgtct
    30061 ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt ttcttcatac
    30121 tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag cttgcatctg
    30181 cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg ctgttcttat
    30241 tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg aatcgaaccc
    30301 gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa tacaaaaaca
    30361 gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc tggtatgttt
    30421 tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg ggaaaagaat
    30481 tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa agcaatgcgg
    30541 agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga aatttccgtt
    30601 tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc tcgaccatcc
    30661 gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa gtaaatgatc
    30721 tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc gaactgcaag
    30781 caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc gcggcagtga
    30841 tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct gtgactttag
    30901 tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt agaatcaaat
    30961 ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac agcgtacgtt
    31021 ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct ttagttcctg
    31081 gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc tcaggagttt
    31141 ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt gcttttattt
    31201 tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt cagttttctt
    31261 ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg cgttcattga
    31321 tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt gttcaaatag
    31381 gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc atcagcgatc
    31441 ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg atggttcctc
    31501 tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa tcctatcgag
    31561 aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct ttaggtcttg
    31621 tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt gacgcaatca
    31681 tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa aaggttgtga
    31741 aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga caagtaaccg
    31801 caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat agcttctcac
    31861 atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc tctatctgta
    31921 aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa attttttcca
    31981 aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta aaccgatcac
    32041 aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata agacgataag
    32101 aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac ttcctgaacc
    32161 tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat aatctctagc
    32221 gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca agctagggat
    32281 aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac cagttaaaaa
    32341 actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca tagagattgg
    32401 caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc tgataaaaga
    32461 agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa caccgattaa
    32521 cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa gaataaaaat
    32581 gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt cttttactaa
    32641 ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat gggttctttt
    32701 tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta ctatcagctg
    32761 ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac ccaataaacc
    32821 ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg aaatcggatg
    32881 ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca tgattccagc
    32941 aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc tatgtttttt
    33001 aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa aaagaataat
    33061 caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc ctagatcagc
    33121 tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa tgatggtcaa
    33181 agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca gaggcaaaaa
    33241 tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac tttgagttaa
    33301 acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt taacaaagtt
    33361 catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa aaagattttg
    33421 agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg ctttattcgt
    33481 gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt gtcgcttata
    33541 catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc ttttctagcg
    33601 tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc aattcacctt
    33661 tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca tgtgtagaaa
    33721 aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa tcaaatgctg
    33781 cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga tctggcagca
    33841 acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg attgtttcat
    33901 tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt tctttttttt
    33961 gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc gtcaattgta
    34021 aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc aatcgatttt
    34081 ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata atactaacta
    34141 aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca aaaatttccc
    34201 cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa cttttactta
    34261 cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt tattgtaccc
    34321 ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat agaaacgact
    34381 aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag gtaagatatg
    34441 agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa attctttagt
    34501 ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt tactatttcg
    34561 tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt taaatcttta
    34621 cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc
    34681 tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct cattagataa
    34741 aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt
    34801 tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta actttggatg
    34861 ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt
    34921 cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt
    34981 tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg
    35041 atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat
    35101 tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat
    35161 tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata
    35221 cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac
    35281 ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt
    35341 tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa
    35401 tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg
    35461 tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac
    35521 tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc
    35581 tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg
    35641 catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg
    35701 gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct
    35761 gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata
    35821 gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat
    35881 ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt
    35941 ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc
    36001 tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc
    36061 tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg
    36121 ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc
    36181 caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata
    36241 ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat
    36301 caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg
    36361 caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta
    36421 gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga
    36481 acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa
    36541 aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa
    36601 ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac
    36661 gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca
    36721 gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat
    36781 tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa
    36841 gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct
    36901 gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag
    36961 cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa
    37021 cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa
    37081 aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata
    37141 cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga
    37201 tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt
    37261 tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac
    37321 aagttgctct gtaaattttt acacaactct tattttttac caaagcactt caattgactt
    37381 tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga
    37441 ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc
    37501 gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga
    37561 ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca
    37621 ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa
    37681 ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta
    37741 gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa
    37801 tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa
    37861 tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga
    37921 aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg
    37981 gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact
    38041 gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt
    38101 ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc
    38161 gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat
    38221 ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg
    38281 tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac
    38341 catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat
    38401 ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta
    38461 aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat
    38521 gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata
    38581 gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta
    38641 ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta
    38701 attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat
    38761 attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat
    38821 acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa
    38881 atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa
    38941 aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac
    39001 agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg
    39061 cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt
    39121 aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa
    39181 tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat
    39241 cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca
    39301 ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg
    39361 gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta
    39421 ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta
    39481 caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa
    39541 ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct
    39601 tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga
    39661 ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa
    39721 gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac
    39781 cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc
    39841 gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg
    39901 tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga
    39961 tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga
    40021 aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc
    40081 aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa
    40141 caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga
    40201 agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg
    40261 atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt
    40321 ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga
    40381 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
    40441 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
    40501 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
    40561 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
    40621 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
    40681 tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt
    40741 tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta
    40801 gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc
    40861 ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt
    40921 gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc
    40981 ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc
    41041 accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa
    41101 caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca
    41161 aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct
    41221 tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt
    41281 tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa
    41341 taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt
    41401 tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct
    41461 tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa
    41521 cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt
    41581 attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg
    41641 ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga
    41701 aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct
    41761 gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat
    41821 tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg
    41881 atctcattat tccattcttt ttccattaat tctttataac attcatttat ccatttttct
    41941 ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata
    42001 attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat
    42061 caattttggg acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca
    42121 ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag
    42181 tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt
    42241 taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt
    42301 tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc
    42361 ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc
    42421 cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg
    42481 caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat
    42541 aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc
    42601 ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac
    42661 caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg
    42721 gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat
    42781 tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc
    42841 aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag
    42901 ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca
    42961 ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta
    43021 accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa
    43081 agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt
    43141 ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc
    43201 taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag
    43261 gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac
    43321 aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt
    43381 tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat
    43441 attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt
    43501 tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac
    43561 gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt
    43621 ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg
    43681 ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc
    43741 cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat
    43801 ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt
    43861 tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag
    43921 taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct
    43981 atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata
    44041 gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac
    44101 agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt
    44161 atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat
    44221 tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt
    44281 gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga
    44341 attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa
    44401 ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc
    44461 atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag
    44521 taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc
    44581 tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc
    44641 tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa
    44701 taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg
    44761 ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca
    44821 taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc
    44881 tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc
    44941 atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt
    45001 catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact
    45061 agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa
    45121 atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta
    45181 gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa
    45241 ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat
    45301 tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta
    45361 cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca
    45421 gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa
    45481 tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa
    45541 ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca
    45601 acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg
    45661 ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata atcaagggat
    45721 aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat
    45781 tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat
    45841 atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt
    45901 gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata
    45961 acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca
    46021 aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa
    46081 gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact
    46141 gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat
    46201 ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca
    46261 gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat
    46321 attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa
    46381 aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc
    46441 gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt
    46501 gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct
    46561 gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct
    46621 agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca
    46681 aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc
    46741 aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt
    46801 gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc
    46861 agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc
    46921 aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga
    46981 ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca atagttcata
    47041 gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa
    47101 tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag
    47161 cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa
    47221 acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg
    47281 agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca
    47341 gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta
    47401 gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc
    47461 tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag
    47521 catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt
    47581 tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa
    47641 atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc
    47701 actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg
    47761 atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc
    47821 atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg
    47881 catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc
    47941 tacattcaca atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc
    48001 attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt
    48061 ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact
    48121 taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat
    48181 gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac
    48241 ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca
    48301 tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga
    48361 aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct
    48421 attccataga tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg
    48481 aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc
    48541 ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa
    48601 cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat
    48661 ctttttctat agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca
    48721 aaattgatta tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt
    48781 ttttcatact atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc
    48841 tgcaaaaaat tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga
    48901 caataccttc aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat
    48961 tagttgatcc aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac
    49021 aattagccca aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga
    49081 atggaaaaag tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg
    49141 tgctagaaaa tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc
    49201 atcatttttc
//
LOCUS       DAYYJC010000010        49121 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000010 DAYYJC010000000
VERSION     DAYYJC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.010"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..872
                     /locus_tag="ACHA9N_000552"
     CDS             60..872
                     /locus_tag="ACHA9N_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9084041.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            869..1669
                     /locus_tag="ACHA9N_000553"
     CDS             869..1669
                     /locus_tag="ACHA9N_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9084042.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1830..3098
                     /gene="rseP"
                     /locus_tag="ACHA9N_000554"
     CDS             1830..3098
                     /gene="rseP"
                     /locus_tag="ACHA9N_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9084043.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3166..4875
                     /locus_tag="ACHA9N_000555"
     CDS             3166..4875
                     /locus_tag="ACHA9N_000555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9084044.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5087..9445
                     /locus_tag="ACHA9N_000556"
     CDS             5087..9445
                     /locus_tag="ACHA9N_000556"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9084045.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9590..10063
                     /gene="rimP"
                     /locus_tag="ACHA9N_000557"
     CDS             9590..10063
                     /gene="rimP"
                     /locus_tag="ACHA9N_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9084046.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10086..11261
                     /gene="nusA"
                     /locus_tag="ACHA9N_000558"
     CDS             10086..11261
                     /gene="nusA"
                     /locus_tag="ACHA9N_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9084047.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11283..11576
                     /locus_tag="ACHA9N_000559"
     CDS             11283..11576
                     /locus_tag="ACHA9N_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9084048.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11573..11884
                     /locus_tag="ACHA9N_000560"
     CDS             11573..11884
                     /locus_tag="ACHA9N_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9084049.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11897..14203
                     /gene="infB"
                     /locus_tag="ACHA9N_000561"
     CDS             11897..14203
                     /gene="infB"
                     /locus_tag="ACHA9N_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9084050.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14230..14577
                     /gene="rbfA"
                     /locus_tag="ACHA9N_000562"
     CDS             14230..14577
                     /gene="rbfA"
                     /locus_tag="ACHA9N_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9084051.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14734..15190)
                     /locus_tag="ACHA9N_000563"
                     /pseudo
     CDS             complement(14734..15190)
                     /locus_tag="ACHA9N_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15355..17717
                     /locus_tag="ACHA9N_000564"
                     /pseudo
     CDS             15355..17717
                     /locus_tag="ACHA9N_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18085..19008
                     /gene="truB"
                     /locus_tag="ACHA9N_000565"
     CDS             18085..19008
                     /gene="truB"
                     /locus_tag="ACHA9N_000565"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9084052.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19012..19953
                     /gene="ribF"
                     /locus_tag="ACHA9N_000566"
     CDS             19012..19953
                     /gene="ribF"
                     /locus_tag="ACHA9N_000566"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9084053.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20305..21090
                     /locus_tag="ACHA9N_000567"
     CDS             20305..21090
                     /locus_tag="ACHA9N_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9084054.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21103..21954
                     /locus_tag="ACHA9N_000568"
     CDS             21103..21954
                     /locus_tag="ACHA9N_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9084055.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22078..22530)
                     /locus_tag="ACHA9N_000569"
     CDS             complement(22078..22530)
                     /locus_tag="ACHA9N_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084056.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22995..23363)
                     /locus_tag="ACHA9N_000570"
     CDS             complement(22995..23363)
                     /locus_tag="ACHA9N_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9084057.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23513..23995
                     /locus_tag="ACHA9N_000571"
     CDS             23513..23995
                     /locus_tag="ACHA9N_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9084058.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24032..25042
                     /locus_tag="ACHA9N_000572"
     CDS             24032..25042
                     /locus_tag="ACHA9N_000572"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9084059.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25157..25870)
                     /locus_tag="ACHA9N_000573"
     CDS             complement(25157..25870)
                     /locus_tag="ACHA9N_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084060.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26043..26650)
                     /locus_tag="ACHA9N_000574"
                     /pseudo
     CDS             complement(26043..26650)
                     /locus_tag="ACHA9N_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            27134..27361
                     /locus_tag="ACHA9N_000575"
     CDS             27134..27361
                     /locus_tag="ACHA9N_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9084061.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27377..28543
                     /gene="hemW"
                     /locus_tag="ACHA9N_000576"
     CDS             27377..28543
                     /gene="hemW"
                     /locus_tag="ACHA9N_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9084062.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28777..29820
                     /gene="hrcA"
                     /locus_tag="ACHA9N_000577"
     CDS             28777..29820
                     /gene="hrcA"
                     /locus_tag="ACHA9N_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9084063.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29914..30477
                     /gene="grpE"
                     /locus_tag="ACHA9N_000578"
     CDS             29914..30477
                     /gene="grpE"
                     /locus_tag="ACHA9N_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9084064.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30528..32357
                     /gene="dnaK"
                     /locus_tag="ACHA9N_000579"
     CDS             30528..32357
                     /gene="dnaK"
                     /locus_tag="ACHA9N_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9084065.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32508..33674
                     /gene="dnaJ"
                     /locus_tag="ACHA9N_000580"
     CDS             32508..33674
                     /gene="dnaJ"
                     /locus_tag="ACHA9N_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9084066.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33756..34385)
                     /locus_tag="ACHA9N_000581"
     CDS             complement(33756..34385)
                     /locus_tag="ACHA9N_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084067.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            34397..34570
                     /locus_tag="ACHA9N_000582"
     CDS             34397..34570
                     /locus_tag="ACHA9N_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084068.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34615..35352)
                     /locus_tag="ACHA9N_000583"
     CDS             complement(34615..35352)
                     /locus_tag="ACHA9N_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9084069.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35354..36856)
                     /locus_tag="ACHA9N_000584"
     CDS             complement(35354..36856)
                     /locus_tag="ACHA9N_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9084070.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37115..39109
                     /gene="uvrB"
                     /locus_tag="ACHA9N_000585"
     CDS             37115..39109
                     /gene="uvrB"
                     /locus_tag="ACHA9N_000585"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9084071.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39119..41935
                     /gene="uvrA"
                     /locus_tag="ACHA9N_000586"
     CDS             39119..41935
                     /gene="uvrA"
                     /locus_tag="ACHA9N_000586"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9084072.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42198..43463
                     /locus_tag="ACHA9N_000587"
     CDS             42198..43463
                     /locus_tag="ACHA9N_000587"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9084073.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43467..44198
                     /locus_tag="ACHA9N_000588"
     CDS             43467..44198
                     /locus_tag="ACHA9N_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9084074.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44333..45217
                     /gene="rapZ"
                     /locus_tag="ACHA9N_000589"
     CDS             44333..45217
                     /gene="rapZ"
                     /locus_tag="ACHA9N_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9084075.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45214..46212
                     /gene="yvcK"
                     /locus_tag="ACHA9N_000590"
     CDS             45214..46212
                     /gene="yvcK"
                     /locus_tag="ACHA9N_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9084076.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46246..47181
                     /gene="whiA"
                     /locus_tag="ACHA9N_000591"
     CDS             46246..47181
                     /gene="whiA"
                     /locus_tag="ACHA9N_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9084077.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47724..48374
                     /locus_tag="ACHA9N_000592"
     CDS             47724..48374
                     /locus_tag="ACHA9N_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084078.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48561..>49121
                     /locus_tag="ACHA9N_000593"
     CDS             48561..>49121
                     /locus_tag="ACHA9N_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFM9084079.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16430 a         8417 c        10569 g        13705 t
ORIGIN      
        1 ttagatattg ctataatact taagatagtg ggaatctttt aagtatagga ggaaaaagaa
       61 tgttacgttt ttttccgcaa aaaaacaaat atattcaaga aaaaagcgaa tttcaattta
      121 ataccgatgg accaattcct cagcacattg ccatcatcat ggacggcaat ggtcgttggg
      181 cgcaaaaccg tcgtttgcca cgagttgctg gccataaaga aggcatggaa acagtaaaga
      241 aagtcacaaa aaaagcatca aggcttggag tgaaagtgtt gacgctttat gccttttcga
      301 ctgaaaactg gaagcgacca aaagatgaag taagttttct catgcaacta cctgtcgact
      361 tctttgatac gttcgtacca gaattgatta aagaaaatgt caaagttcat gtcatgggtt
      421 atgaaaacgt attgccagaa catacgcaag atgctgtaag acgtgcaatt gagcaaacaa
      481 aaaacaatac tggaatggta ttgaattttg ctttgaatta tggaagcaga gcagagatcg
      541 tgactgctgt ccaagaaatt gccgaagaag tagcaaaagg agaaatacac gcagaagaaa
      601 tcgatgatga attgattgct aagcatttaa tgacaggttt cttgcctaaa gagttacaag
      661 atcctgagct gatgatccgt acaagtggag aagagcgaat tagtaatttt ctgttatggc
      721 agatcgcata tagtgagctg tattttacaa aagcgctttg gcctgatttt gatggtgcgc
      781 atcttgaaga agcaatcgcc tcttatcaaa atcgagaccg tcgattcggc ggtgtaaaaa
      841 aaacagaaga tgagaaaggg gatcagtcat gaaacaacgt gtggtcacag cagccgtggc
      901 gctagtcttg tttattccga ttatatggat cggaggcata gcggttgagc ttacagcagc
      961 aatcttagca gtaataggag tttacgagct gtttcggatg aaaggattga cactcttaag
     1021 ttttgaagga gttttatccg ccgttggtgc agtgttactt gttttgccaa aagaaagatg
     1081 gtttttcttt cttccagaga atgtaagtac atttatgtta ttttatctta tagttatgat
     1141 tcttttagga acatcagtta tttctaaaaa tacgtatacg attgatgaag caggttttcc
     1201 tgtgcttgtc agcttgtatg tgggaatagg tttccagaat tttgtcaatg cacgaacaga
     1261 aagcttggct gttttgatat ttggtctatt tattgtttgg gcaactgata tcggagcata
     1321 tatggttggt cgacgttatg gtcagcataa gttaagtcct gatatttcgc ccaacaaaac
     1381 gattgaaggt gcgctcggtg gtattggtag tgctgttgta gtcgcatttc tttatttatt
     1441 gctataccca gcaaaagaac tatttaatca tggtatgttt gtcatgcttc tgatgacgat
     1501 tattttttca gcagtcggac aatttggtga tttggtcgaa tctgcaatca aacgtcatta
     1561 tgatgtgaaa gactcgggaa atatcttacc aggccatggt ggaatcttag accgtttcga
     1621 cagtttgctg tttgtatttc ctatcatgca tttatttggc attatttagt aagtatacct
     1681 gcgtttcttt gtgaatttca taaaagacgc aggtttttat ttttcattat attgatacta
     1741 ttggtgtctt tggtatacta tggtaaaatt atagtgtttt atcgttgttt tataaaaaac
     1801 acaaaaaaca agtaaaggag aaaaaagcta tgaaaacgat tctgacattt atcatcgttt
     1861 ttggtatatt agtgattgtt catgagtttg gtcatttctt ctttgcgaaa cgatcaggaa
     1921 tcctcgttcg cgaatttgct atcggtatgg gtccgaaaat ttatgggcat caagcaaaag
     1981 acgggacaac atatacgctt cgcctattac ctattggcgg ttatgttcga atggcaggca
     2041 atggggatga tgaaacagaa atggcaccag gaatgcctct ttctctttta ctaaattctg
     2101 acggcatcgt agagaagata aatttaagta agaaaatcca attgacaaat gctatcccga
     2161 tggaattgag ccgttatgat ttagaagatg agctgactat cactggttat gtgaatggag
     2221 acgaaacaga agtagtaacc taccctgttg atcacgatgc gacgattatt gaaaacgacg
     2281 gaacagaaat cagaattgcg ccaaaagacg tacagttcca atcggctaaa ttgtggcagc
     2341 gtatgctgac caactttgct ggacccatga ataactttat tttggcaatc ctgttattca
     2401 tcattctggc atttatgcaa ggtggtgttc aagtgacgaa cactaatcgc gtaggagaga
     2461 tcatgcctaa tggagctgcg gcagaagcag gcttaaaaga aaatgatgag gtagtcagtg
     2521 tggacggaaa agaaatccat tcatggaatg atctgacaac agtcattacg aaaaatccgg
     2581 ataaaacatt agactttaag atcgaacgag aaggtcaggt gcaatctgtc gacgtgacac
     2641 cgaaatctgt agagtcaaat ggcgaaaaag ttggccaact tggtataaaa gccccaatga
     2701 atacaggttt tatggataag atcattggag ggacgcgtca agctttcagc ggttcattgg
     2761 aaattttcaa agccttagga tcactattta caggtttcag tttagataaa ttaggcggac
     2821 ctgtcatgat gtaccaattg tcttcagaag cagcaaatca aggtgtaaca actgttatta
     2881 gtttgatggc gcttctttca atgaatctcg gaatcgtcaa tctgcttccg attcctgcct
     2941 tagatggcgg gaaattagtc ttgaatattt ttgaagggat tcgcgggaaa ccattaagtc
     3001 aagaaaaaga aggaatattg acattagctg gctttggatt tttaatgttg ttgatggtgc
     3061 tagttacatg gaacgatatt caacgattct ttttctagaa aaagttgaca aatagggtat
     3121 aataacaaac gataactatt ttgaggaaat aaaggagtat atagaatgag acaatcaaaa
     3181 atgttgattc caacattacg cgaagttcca aatgatgcag aagtattaag ccatcaaatc
     3241 ttgctaagag ctggatatat ccgccaagta tcagcgggta tttattcata tctgccatta
     3301 gcaaatcgtg tattggaaaa actgaaacgc attatgcggg aagaatttga aaaaatcgga
     3361 gcggtagaaa tgctgatgcc tgccatcttg ccagcagaac tttgggaaga atccgggcgt
     3421 tacgaaacat atggaccaaa cttgtatcgt ttcaaagacc gcaatgatcg aaaaatgatc
     3481 cttggaccaa ctcacgaaga aacttttact gatttgatgc gtaatgaaat caattcatat
     3541 aagaaattac ctttaaatct ttatcaaatc caacctaaat atcgtgacga aaaacgacct
     3601 cgcttcggtt tgcttcgcgg aagagagttt atcatgaaag atgcgtattc tttccatgca
     3661 acggaagaaa gtttggacga gacatataaa gattacgaaa aagcctacac cgaagtattc
     3721 aagcgttgtg gcttagaatt tcgctcgatt atcggagacg gtggcgcaat gggcgggaaa
     3781 gactcaaaag aattcatggc gatctcagaa atcggggagg atacgatctg ttattcgact
     3841 gaaagtgatt atgctgcaaa cttggagatg gctacaagct actatgtatc aaaaaaatcg
     3901 catgaaacac agcttgaatt ggagaaagta gctacaccta acgtaaaatc aattgacgaa
     3961 gtagcagatt tcttttctgt tgaacctcaa aaaatcatca agtctgtatt gttcattgcg
     4021 gatgaacaac ctgtccttgt tttggtacgc ggagaccatg aagtcaatga cgtaaaactg
     4081 aaaaactttt tatctgctga ctttttagaa gaagcaacag aagaagaagc acaaaaatat
     4141 ctaggtgctg agtttggttc tgtcggacca gtcggtgttt cagatgacgt aaaagtatat
     4201 gcagaccgtt atgtccaaga tttagcgaat gccattactg gagcgaacga aacaggattc
     4261 cactataaaa atgtgaatcc agaacgcgat ttcactgtac tttcttatga agatcttcgt
     4321 tttgtacaag aaggagatcc ttcaccagac aacaatggtg ttcttgcctt tactcgagga
     4381 atcgaaatcg gacatatctt caaaatcggc actcgttaca gtgaatctat gggagcaact
     4441 gtactagacg aaaatggacg agaaaaattt gtgattatgg gctgttatgg tatcggtgta
     4501 agtcgtttat tatcagcaat cgtagaacaa cattcagatg aacaaggaat caactggcca
     4561 aaaggaattg cgccatttga tttgcatatc gtccaaatga atctaaaaga cgaataccaa
     4621 acaaacctga cgaatgaact agaagaagcg atgacaaaag ctggctatga agtactggtt
     4681 gatgacagaa atgaacgcgc aggagtgaaa ttcgcggatt ctgatttgat tggctgtcca
     4741 atcagaatca ctgtcggtaa aaaagcagtg gatggaatcg tagaagtgaa aatcaagaaa
     4801 actggcgaaa tggttgaagt acgtaaagaa gaattggtga atactttacc aatcctcttg
     4861 aaccaaggcg aataataagc gttaaaacac agaaaataaa aatagctgaa tcagctgttt
     4921 ttgacaatct gatgattcgc ttttaaaatt ggaactgcga tttgaatcgc agttccaatt
     4981 ttttaatgta aaactcagaa aaaacgagta tactaaaagg tactgaaaaa gaagaggata
     5041 agatgcgatc aattgcattg tttctcatca gaaagggcgt atatctttgt cagaaaaacg
     5101 agatttattt gaaaaactgt tggaacaaat ccaactagag gaagcagaaa aaaatcatcc
     5161 actgcttact gctggtgaaa tggatagtgt agtggtccat cgtaaatcgc gtttatggga
     5221 atttacgctg catttttcca aaattttgcc tattatgcta tatcggtcat tgactcagca
     5281 tctgactatc gcttttaaag atattgcaca agtcaaactg aatatcttag cagatgacca
     5341 gacattcgat gaacagctct tgcaagatta ctgggcccaa gctttggaaa atcagcaatg
     5401 cgatacacca ctagcacagc aagtattgaa aacgcaggta cctgtgataa aagatcgtaa
     5461 agtcatcttg ccaatcgaca gtacaggagc tatttcttat ttgaagcaac aatacttgcc
     5521 attagtagaa gagttgttcg tcagttacgg attcccaaaa tttcgtatcg aaccagaagt
     5581 ggatgagcag caagcggaac gagtattgaa actttttgaa gaacgcaaac aagaacaggc
     5641 agaagccttt atgaagcaag cagctgaaag tctagttgtc cacgaacaaa agaaaaagga
     5701 aagaaaagag caatcgccag ctcttgaagg acccatccac atccagctag gacgaaatat
     5761 tccagccgat gaaccaacga caccaatgat cagtatcgtt gaagaagaac gaagagtcac
     5821 acttgagggg tacgtattcg acaaagaagt cagagaacta cgttcaaaac gcaagatctt
     5881 gactttgaaa atcacagatt atacttcatc atttatcgtc aaaaaattct cgaacggcga
     5941 aaaagatgaa caagtatttg atgcgatcag tgtcggtagc tggttaaagg tacgaggaag
     6001 tattcaagaa gatacgtttg tccgtgattt ggtcatgaat gcgcaagata tcatagaagt
     6061 caaacataca cctcgcaaag attatgctcc tgaaggggaa aaacgagtag agcttcatgt
     6121 tcatagcaat atgagtacga tggatgcaac aaacagcatc tctgatcttg tggcacaagc
     6181 agggaaatgg ggacaccgag ctatcgccat tacagaccac ggtggtgcgc aagcctttcc
     6241 ggaagcacat agtgctggaa aaaaagcagg tgtgaaaatc ttatacggcg tggaagcgaa
     6301 tgtcgtagat gatggtgtcc caattgctta taatgatgca catgaagcat tgagtgaagc
     6361 cacatatgtc gtatttgacg tggaaacgac aggattgtct gcagtatatg acacgatcat
     6421 cgagctagcc gcagtcaaaa tgtataaagg aaatgtgatc gaaagctttg atgaatttat
     6481 cgatccagga catcctttgt cacgaacaac ggtagatctg actggtatca cggatggaat
     6541 ggttcgagga tcaaagtcgg aagaagaagt attgcggatg ttccttgaat tttcaaaaga
     6601 tacaattttg gtagcccaca acgcggcttt tgatatgggg ttcttgaata ctagctacgc
     6661 aaggtatggt attcctgaag cggccaatcc agtcattgat acgttggaat tagctagata
     6721 tctgtatcct caattcaaac gtttcggatt aggtgtgtta tctaaaaaat ttggtgtcag
     6781 cttggaacag catcaccgag caatctatga tgctgaagca actggtcact tagcatggat
     6841 ctttgtgaaa gaggcaatgg acaatcataa catgctttat catgatcagc taaatgagca
     6901 tatcggagaa ggagattctt acaaaagggc tcgacctttc catgtaacga ttttagcaaa
     6961 aaatcaggca ggactgaaag atttgttcaa actgatttca atgtcgaatg tcgaatactt
     7021 cgaacgcgtc ccacgaatac cacgctccca attgaaaaag atgcgtgaaa atctattgat
     7081 cggctcagct tgtgataagg gagaaatatt cgaagccatg atgcaaaaag gggtagaaga
     7141 ggcaagaaac cgagcaaaat tctacgatta tattgaagtt atgccaaaag cggtctatgc
     7201 gcctttgatc gagcaagaat tggtaaaaaa tgaacatgat ttggaagaaa ttatccaaaa
     7261 tttggtagaa atcggaaaaa gtttggataa aatcgttgtt gcaacaggta atgtacatta
     7321 tctgaacgaa gaagatgcca tttaccgaaa aattttgatc aattctatgg gcggagcgaa
     7381 tccgttgaat cgtcatagtt tgccggatgt ccatttccga acaaccgatg agatgctaac
     7441 tgcatttcac tttttagggg aagaaacagc gaaagagatc gtagtagaaa atacgaataa
     7501 aatcgcagat atctgcgaag aagtgattcc agtcaaagat gaactttata caccgaagat
     7561 tccaggttca gaagatgaaa tatcagaatt gagttacaca aaagcaaaac aaatgtacgg
     7621 agatccgtta cctgagatca ttcaaaaacg attaaaaaaa gagttgaatt ctattaatgg
     7681 taatggcttc tcggttattt atctgatcgc ccaaaaacta gtgcataaaa gtaatgaaga
     7741 tggctatcta gtaggttcac gtggctctgt tggctctagc tttgttgcga cgatgacagg
     7801 aatcactgaa gttaatccgt tagcccctca ttattattgt ccagagtgcc aatattccga
     7861 gttttttgaa gatggtacgt atggttctgg atttgacatg ccagaaaaac aatgtccaaa
     7921 atgtggtgca cgcttaaaca aagacggtca tgatattcca tttgagacat ttttaggatt
     7981 ccatggagac aaagtgcccg atattgattt gaacttctca ggtgattatc aagcagaagc
     8041 ccataactat acgaaagtat tgtttggcga agactatgtt taccgtgccg gaacgattgg
     8101 tacagtcgcc gacaaaacag catatggcta tgtaaaagga tacgaaaggg acaataatct
     8161 gcagtttcga agtgcagaag tcgacagatt agcaaaaggc gctactggag tcaaacggac
     8221 aaccggccag catccagggg ggatcatcgt tattcccgat tatatggatg tctatgattt
     8281 tacgccgatc caatatccag cagacgatca aaattcggaa tggaaaacga ctcattttga
     8341 ttttcactcg attcacgata atgtattgaa attagatatt ctagggcatg atgacccaac
     8401 tgttattcgg atgcttcaag atttatcagg gatcgatcca caaacgatcc ctacggatga
     8461 cccggaagtg atgcggatat ttgccggtcc tgaagtactt ggagtaagtc aggaacaaat
     8521 ttattccaaa acagggacat taggtattcc agaattcggc actcgttttg ttcgagggat
     8581 gctagaagaa acgcatccta caacttttgc agaactactg caaatttccg gactatctca
     8641 tggtacagac gtatggttag gaaatgcgga agaattgatt cgacgaggag atgcgacact
     8701 ggcggaagta atcggctgtc gtgatgatat tatggtttat ttgatccatg ctggattaga
     8761 tagcgggatg gcatttaaaa tcatggaaac tgttcgtaaa ggacaatgga acaagatccc
     8821 tgatgaattg cgagaaacct atctatcggc gatgaaagaa aataatgtgc ccgactggta
     8881 tatcgattct tgttcaaaaa tcaaatatat gtttccgaaa gcccatgctg cagcttacgt
     8941 attgatggct cttcgtgtcg cttatttcaa ggtttatttc cctattttgt attactgtgc
     9001 atacttttct gtacgtgcag acgactttga tctagtctcc atgtgcaaag gaaaagatgc
     9061 tgtgaaacaa gcaatgaaag aaatcacgga caaaggatta gatgcttccg taaaagaaaa
     9121 gaatcaactg acagtgctgg agttagccaa tgaaatgctg gaacgaggat tcaaatttgg
     9181 aatgattgat ttgtataaat cggatgccgt taactttgtt attgaaggtg atacgttgat
     9241 cgcaccattt agagctgtcc caagtttggg aaccaacgtg gcaaaacaaa tcgttgaagc
     9301 aagaaaagac ggtccatttt tatccaaaga agatcttgcc acccgcggta aagtttcaaa
     9361 aacattgatc gaatatatga acgacaatgg cgtattgaaa gatctaccag atgaaaatca
     9421 attatcgtta ttcgatatgc tataagcatc tgttttcctg tctgcttgta attttaagca
     9481 gagtatggta tagttcttat aggtaatgat actaactgtg agtgagcgga tttttcgctc
     9541 actcttttta atggaattac gcacttaatt ttttaaggag gcgaaacttt tgagtagcgt
     9601 cgttgaaaca gtcacagaaa tggtgacacc gattttagat gaacaaaatt ttgaacttgt
     9661 cgaagtcgaa tttgtaaaag aaggaaaaag ctggttctta cgagtattta ttgacaaaga
     9721 aggcggtatc gatatcgaag aatgcgcctt tgtcagtgag aaactgagtg aaaaacttga
     9781 tgcagcagat ccagatccga ttcctcaagc ttatttcctg gaggtatcct ccccgggagc
     9841 agagcggcca ttgaaaaaag aaagcgacta tgaaaaggca cttggtgatt atgtccatgt
     9901 ctccttatac caaccagtag atggcgaaaa acaatatgaa ggatttcttc aatcatttga
     9961 tgctgaacag cttacattga aaatacgcat aaaaacacga gaaaaagaat tgacttttga
    10021 ccgaaaaaat attgcaaaag ctcgcttagc tatccaattt taatcacgga gaggaatcag
    10081 aaaaaatgag taaagaaatg ttaaatgcgt tagacgcttt agaagctgaa aaagggatct
    10141 caaaagaaat cgtgatcgat gctttagaag cagcattggt ttctgcatac aaacgtcact
    10201 atggccaagc acaaaacgtc gaagtggaat ttgatcagaa aaaaggaaag atccatgtct
    10261 atgcggtaaa agaagtaacg gaagaagtga tggattcaca gttggaagtc tcattgaaag
    10321 atgcactttt aatcaatcca gcatatgaga tcggtgatac gatccgtttt gaagtaacac
    10381 caaaagattt tgggcggatc gctgcgcaaa cggctaaaca agttatttta cagcgtgtac
    10441 gtgaagcaga aagaacgatt atttataacg aattcagtgc ttatgaaaaa gacatcatgc
    10501 aaggaatcgt cgaacgccag gataaacgtt atatttatgt caatcttggt aaaattgagg
    10561 cagttctttc taaacaagat caaatgccaa atgaatttta tcaaccacat gatcgaatca
    10621 aagtttatgt ttcccgagta gaaaatacgt ctaaaggtcc gcaagtattt gttagccgca
    10681 gccatccaga cctattgcgc cgcttatttg agcaagaagt accagaagtc tatgatggaa
    10741 tcgttgaaat tgtcagcatc gcccgtgaag ctggcgatcg ttcaaaagtg gcagttcgct
    10801 caaatgatcc aaatatcgat gcagtcggta catgtgtcgg gccaaaggga caacgagttc
    10861 aagcaatcgt caatgaatta aaaggcgaaa acatggatat cgtcgaatgg aatgaagatc
    10921 cagctgtatt cattgcaaat gcattgaatc ctgcacaagt catggatgtg atcttcgatg
    10981 agacaaatcc aaaggcctgc acagttgtcg ttccagacta ccaattatcg ctagcaatcg
    11041 gtaaacgcgg acaaaacgca cgtttagctg ctaaattgac taaccataaa atcgatatca
    11101 aatcagaatc tgacatgacg gaattttatg aaaatcaaga acaacaaaaa gaaacagaag
    11161 aactgcatga tgaagcaatc gttcagtcag atttaacaga cgatgaatat gaaacaattg
    11221 cattcaacaa tgaaacggtc gaagaaaaac cagaagctta actttagtaa ggaggcaaag
    11281 agatgaaaca aaggaaaatt cctttacgca aatccgtggt ttcaggtgaa atgaaaccta
    11341 aaaaagagat ggtccgaatc acccgttcaa aagaagggga agtatcgatc gatccaactg
    11401 gaaaaatgcc tggacgagga gcgtatgtct cacttgagcc ggaagaagta caacaagctt
    11461 gggataaaaa aattctcgat cgagtactcg aaacaacatt aacagatgag ttttatcaag
    11521 aacttcttga ctatgtcact caccaaaaag cacgtaaaga gttatttgga aaatgaatac
    11581 agttaatcgg caaaaagcca tgaatctcat cggtctagcg atgcgcgcgg gaaaactaat
    11641 cacgggtgaa gaactgacca ttgctgaaat ccgcaaacaa aaagcaaaat tggtgtttgt
    11701 ggcaaccgat gcaagcgaga atacaagaaa aaagataaaa gataagagtt catattatga
    11761 agttccttgc tttgagctat tttctgaagc agaaatcacg cagatgatcg gaaaacctcg
    11821 caaggtattc ggaataatgg atgccggatt tgcgaagaag accaaggagc taattgaagg
    11881 ttaggaaggt gattgcatgg gcaacaaaag aatctatgaa ttagcaaaag aactcaaaaa
    11941 atctagtaaa gacgtcgtgg aaaaagcaca acaacttggc atcgatgtga aaaaccatat
    12001 gggtgcgatt actagcaatg atgagaagaa attgcaacaa gcgttcaaaa acccaagcac
    12061 gaaacaacaa gctgcacaac aagcaagcag aaaaccagcc aatcaacagc cgaacgatca
    12121 acgtaaaaaa gaaacaacga acaatcaaca aaaaaataaa aataaccgca gttatcaaga
    12181 tcgcggtcaa gggagcggtc aagtgaatca aggaaaaaaa ccaacaacaa accaaaataa
    12241 tacgggaaat accagcagcc aaaatcgtca aggaacgagc caaggcggca atcaaaaccg
    12301 tactaacaat aacaacaaac caaacaataa caatcgcggc aaatttaata ataataaccg
    12361 taatcgttat aataaaaaag gtaaaaaagg caaacagcaa caatcaacta aaccagctgt
    12421 accaccacgc aaattccgtg aattgccaga tgtattagag tatactgaag gtatgaacgt
    12481 agcagatatt gcgaaaaaaa ttcatcgtga gccagcagaa atcattaaaa aattatttat
    12541 gcttggtgtc atggtcaatc aaaaccaagc actggataaa gatacgattg aactgttagc
    12601 gacggactat ggcatggaac cacaagaaaa agtccaagtt gacattgctg atatcgataa
    12661 attcttcgag gcagaagaag tcgatccaga taaattagtc tcacgtccgc cagtcgtaac
    12721 gattatggga cacgtagacc atgggaaaac aactttattg gatacattac gccattcacg
    12781 tgtaacaagc ggcgaagccg gcggaatcac acagcatatc ggtgcctacc agatcgatat
    12841 tgatggtaaa ccaatcactt tcttggatac accaggacat gcggccttta caagtatgcg
    12901 tgcacgtgga gcaagtatca cagatatcac gatcttagtt gttgctgcag atgatggcgt
    12961 catgccacaa acagttgaag caatcaacca tgccaaagct gcaggcgttc caatcatcgt
    13021 cgctgtaaat aaaattgata aaccaggtgc aaaccctcaa catgttatgc aagaattaag
    13081 tgagtatgag ttaattcctg aagcatgggg tggagaaacg atctttgttg agatctcagc
    13141 gaaattcggc caaaacatcg aagaattgct ggaaatgatc ctgttagtcg cagaagtaga
    13201 agatttaaaa gcagatccaa cacaacgcgc aatcggaaca gtgatcgaag cacgtcttga
    13261 taaaggtaaa ggtcctgtgt caaccttact tgtccaagaa ggaacacttc atgttggtga
    13321 cccaatcgtt gtcggtaata cttacggacg tgtccgtgta atgacaaacg atcttggccg
    13381 tcgcgataaa gaagcaggac ctgcaacgcc agtagaaatc actggcttga atgatgtacc
    13441 tcaagcaggt gaccgtttcg tagtcttcga agacgaaaag acagcacgtg cagcgggtga
    13501 agaacgcgcg aaacgtgcat tattagaaaa ccgtgctgcc acaagccgtg tgactttgga
    13561 taacttattt gaaagcctga aagaaggcga attgaaagaa gttaacgtaa tcatcaaagc
    13621 agatgtacaa ggttcagcag aagcactggc tgcatcattg aagaagattg atgtggaagg
    13681 tgtccgtgta aaaatcgtcc attcagctgt cggagcaatc aatgaaagtg atgtaacctt
    13741 agcagcagcc agcaacgcta ttatcatcgg tttcaacgtt cgtcctacac cgcaggcaaa
    13801 agtgcaagct gatactgaag aagtcgatat tcgtcttcac cgtatcatct ataaagccat
    13861 cgaagaaatc gaaactgcga tgaaagggat gctagaccct gaatttgaag aaaaaatcac
    13921 cggtcaaatg atcgttcgtg aaaccttcaa agtttctaaa gttggtacga ttgcaggtgc
    13981 ctatgtaaca gaaggatata ttcgtagaga tagcggtgtc cgtgtaatcc gtgacggaat
    14041 cgtaatctat gaaggccaac ttgcaagtct gaaacgattc aaagatgacg tgaaagaagt
    14101 caaaatgggt tatgaatgtg gtgcaatgat tgaaaaattc aatgacatca aagtagatga
    14161 cgtaattgaa ggatttatca tggaagaagt caaaaatgac taaaaactga taaaaggagg
    14221 ccaatcatta tggctaacta ccgtgaccgc cgagtcggtc aagaaatctt aaaagaagtc
    14281 aatgatattt tgcgcaaaaa ggtccgtgac cctcgtgtgg agaacgtaac gatcacagat
    14341 gtgaaagtta cgggggactt acagcaagca acgatctatt acagcatttt atctgactta
    14401 gcatcagata agaaaacagc acaagatgga ttgaataaag catcaggatt gattcgtcga
    14461 gagttagggc acaatctaag tatttataag acacctgaat tgttctttga acttgatgaa
    14521 tcagttgtct atggcaacca tatcgatgaa ttgcttcgcg acttgaacaa agactaatca
    14581 aaaactcccg caaatcctgt gaaatcaggg gttgcgggat ttttttgttt tcattaagac
    14641 gtattaccta agagttccat gactttagtc ttgcggcgtg tcagctaacc aaaaaagaga
    14701 aaaagaaaag taaaaaattc gactttcttt tttctagtag tatataaatt tttgcatata
    14761 gataaagatc aagataccga taacggaagc tagattgtaa tctattttag tgacagtcag
    14821 gtatagccaa gtaactagaa gatcagtgga acctgcacca taaaagtttg agtttgtcgg
    14881 tccgttgaca gttaaaagaa aaatcacgtt gaagttattg atatttccga ttacgccgct
    14941 tcgatttggt cacgatccaa attttggata atccctgtag aaattagcat agtagctggg
    15001 ataccaattc acatattgac aaagataaca gagactttta cataaaacgg atcaatcagg
    15061 aaaggaattg ctcgatccag ccccagtttt ccagcaatgt gttaactggc ccagcgtcat
    15121 tcagcaaatt atgcatgatg aggagcgaaa tgaactgcgg aacagccatt gtgataacga
    15181 caagtttttt ggatcaatgt aaaaaacgtc agcgaaagga aaaatttcgt ggttttttat
    15241 acctgttcat ttggctagat ataaaatcca aacaaatgaa cagaagtgct tattaggttt
    15301 caattgggag tatctgcgct agaataagag taataatgaa aaggagtgtt atgtatggat
    15361 tattctagta aagaatactt tgataaaatg acggcttggt ggcgtgctgc taattatctt
    15421 tctgttgggc aactttatct taaagataac cccttactga gacgtacact gaaacccgaa
    15481 gatgtcaaaa aacatccaat cggtcattgg ggaacgattc ctggccaaaa ttttatttat
    15541 gtccatttga accgagtcat caataaatat gatttgaaca tgttttatat tgaagggcca
    15601 ggacacggcg gacaagtcat ggtatcgaat gcttatttag atggaagtta cacagagatc
    15661 tatccagaag taacggaaga tgagacagga atgcaaaaat tattcaaacg attttcattt
    15721 cctggaggca ttgcttctca tgcagcacca gaaacaccag gttctatcca tgaaggcgga
    15781 gagcttggat actctctttc acatgcagtc ggagctgtat tagacaatcc tgaagtaatc
    15841 tctgctgtcg tgattggtga tggtgaagca gaaaccggtc ctttagctgg aagctggttc
    15901 tcaaatgtct ttatcaatcc agtgatagat ggagctgttt taccgatcct tcatttaaat
    15961 ggtgcaaaaa tagccaaccc aacgatcttg gcacgtaaat cagatggaga attagcaaat
    16021 tactttaacg gacttggctg ggaacctttc tttattgaag gaaatgattc agaaaaatta
    16081 aatcctgtta tggctgaaaa aatggatcaa gcaatcgaga aaattaaatc catccaaaaa
    16141 gaagctcgcc tgaagacagc aacagatgtc gtgatgccaa aatggccagt attgatcgta
    16201 cgcacaccaa aaggatggac cggtccaaaa gaatgggacg gggaaccaat tgaaggaacc
    16261 ttccgagcac accaagtacc gattcctgta gatcaagaac atatggatca tgcagatgct
    16321 ttactccgtt ggctgaaatc ctatgaacca gaaaaattat ttgatgcaca aggacgtatt
    16381 ttagaagaaa tcagagaaat cgcgcctact ggtgaccaaa gaatggcaaa aaatcctatc
    16441 accaatggtg gaatcgatcc aagaccgctt attatgccag attggaaaaa atatactttg
    16501 caatttgaaa aaccaggctc cgttaaagca gaagatatga ccgaacttgg taaattcgtt
    16561 cgcgagatca ttgagaaaaa tccagaaaat ttccgtatat ttggaccaga tgaaacaaaa
    16621 tcaaaccgat tgaatcaagt tttcaaaacg acaaatcgcc aatggatgga aaaaatcgaa
    16681 ccagaaaatg atgaatggct ttcgccatct ggtcgcgtca tcgacagtca attatcggaa
    16741 caccaagatg aagggttctt agaagggtac gttctgactg gacggcatgg ttttttgcaa
    16801 gttacgaatc attcttgcga gtggttgact ctatgttgac tcagcacttc aaatggatgc
    16861 ggaaatcaca tgacctttct tggcgaaatg attatccttc gctaaatctc attgcaagct
    16921 caacagtctt ccagcaagat cacaatggat atagccacca agatccagga atattgacac
    16981 atttggcaga aaagaaggcg gagttcatcc gtgaatatct gcctgcggat gcaaatacat
    17041 tgctcgcagt catggataaa gcattccgct cgtcagaaaa aatcaatttg atcatctctt
    17101 ccaaacatcc gcgtgctcaa ttttactcag cagaagaagc agccgtctta gtgaatgaag
    17161 gcttgaaaat cattgattgg gcttcgacag caaaagagga ggaacctgaa ttggtcattg
    17221 ctgctgcagg aacggaatct aacttagaag cattagcagc tgtaactttg ctgctggaag
    17281 aattcccaaa actaaaaatt cggttcatca atgtcgtaga tttattgaaa ttacgtcatc
    17341 caagtcaaga tccgcgtggg ttgagcgatg aagaatttga tcaatatttt acaaaagaca
    17401 aaccgatctt atttgctttc cacggttatg aaacattggt ccgcacgatt ttctttgatc
    17461 gtcataacca tcatttaatg attcatggct acaaagaaaa tggagatatc acgacaccat
    17521 ttgacatgcg tgtagtaaat gaacttgatc gttatcattt ggcaaaagac gcagctttaa
    17581 aaatcaaagg aagtcaagca gaagattttg cgaaaaaaat ggatcagaaa ttacaagaac
    17641 atcaaaacta tattcgtgaa aatggtattg atctgccaga agtattggat tggaaatgga
    17701 aaaatcttga ccaataaaat gataaaatcg atcccgcaaa atagaatttt ttacattgat
    17761 taagaaactg aaaaaataaa taaaaaaaac ggaaaaaaga ctggaagaat ggccagtctt
    17821 ttttttcgtt tttgggaagt gaaaaccaaa ctatttacct gtaagccacc cataaatcca
    17881 atcaaatggt gcaaagaaaa aatcttccat tctattccct cctttaaaaa agcataacat
    17941 gatttctatt tacgtgagca tgaattttgt tacgttttta ttgccgaaaa ataaaaaccg
    18001 ttcattatga tgtcaaattc gtcagtaatt gatgcaatgt gttataataa ttcagttaaa
    18061 tcaaaaatga aatggagaaa agacatggac gggttattac ctttatggaa agaacgcgga
    18121 atgacaagtc atgattgcgt attcaaatta agaaaaatct tacatacgaa aaaaatcgga
    18181 catgggggta cgcttgaccc agacgtagac ggtgtgttgc ccatttgtat tggaaaagcc
    18241 acaaaagtta tcgaatattt gacagattct gggaaaatct atgtaggcga gataactcta
    18301 ggtttcagta caacgacgga agatcgatcc ggagaacggg tagaaacaaa accagtagat
    18361 atgtctttat ctgaaaaaaa aatagatgaa gtgatggctt catttattgg tgagatcacc
    18421 cagataccac caatgtactc agcggtaaaa gtcaacggta agcggttata tgaatatgcc
    18481 cgcaataatg aaacagtgga gcgtcctgtt agaaaggctc agatttaccg ttttatccgg
    18541 acaagtgaat tgacaagaga cgaagtgaca ggaaccattt cttggcgctt cgaagtagaa
    18601 tgcgggaaag gaacctatgt ccgcacatta gctgtagata caggcaagaa acttggttac
    18661 cctgcgcaca tgtctgatct gacccgaaca gcaagtgccg gtatgtccca gaaggatgct
    18721 ctgacattag aagaagtagc tcaagctgtg aaaaatggga caatcgatga ttgtttgctg
    18781 ccaatagaaa aaggagtgga acggttcaaa cgagtagata ttactgatga agtatggcag
    18841 aaagtaaaaa acggtatgag acttgattat actgtatttg cgctcccaga aatgccgtca
    18901 gaagaaattg ctttgtttta taaaggaaaa gttgtcagta tttatcagcc aaacccgaaa
    18961 gaaaaaaaca aattaaaacc tagtaaagta ttgagaaatg aggaataacg aatgaatatc
    19021 atcaagatca gacatcctta ccaatcagaa caaattccag aagacgatgt cgttttagtg
    19081 ctgggatttt tcgacggcgt gcatatcgga caccagaaag tcataaagac aggtatggaa
    19141 attgcacaaa aagaaggatt gaaattagca ttgatgactt tcaatcagca tccatctatt
    19201 gtctttaaaa agatcgatcc tagcagcgtg aaatatttga ctagccttaa gcaaaaagaa
    19261 gaaaaaatgg ccgctttggg tatagattat ttatatgaag ttgattttac ttcttctttt
    19321 gctcatttag caccgcagga atttgttgat caatacatcg tagggttaca tgcaaaatat
    19381 gctgtttccg gttttgatta tacgtatgga ccaaaagaca ttgcagatgt cgctcatttt
    19441 cctgaatatg ccaaaggaag attcaaaatc gtcacagttt ctaaagaaga agaacaaggt
    19501 gaaaaaatca gctctacccg tattcgtgga ttactagaca gcggacaagt aaaagaagca
    19561 gcaaaactat taggttgcac atatgaggta gaaggggtag ttgtccatgg cgatgcacga
    19621 ggcagactac taggattccc tacagcaaat gtcaaagtaa agagcacggt tcatttgcct
    19681 aaagaaggtg tttatgtgtc ggaaataaaa attggggaaa catggtatcc agccatgggt
    19741 tcaattggac ataatgatac attcggtgaa ggacgggagc tgactgtaga gttgtatatt
    19801 ttggacttta atcaagatat ctatggtgaa catgtcgcgg tacgctggaa tcaattttta
    19861 agagatcaag tgaaattttc tagtgtagag gaattgattg aacaactcaa ggaggatgaa
    19921 caagcaacag ctgaatattt ttctcagcaa taaaacatta tgcataaaat ctcttgttta
    19981 tgtataacta gcaattacaa taaaatgggt gataaataag cccaggaaag gggctgtgac
    20041 aaaagtcttg tcacagcctt tttttccaaa taaatggttt ttcagaagta gcttcttcgg
    20101 aaataagccg aaatttcttg aagctgacca cttctgtcac aacctctttc tttatagaaa
    20161 taaaaggaca ctagcaattc atgaaaacga ttgctagagc gtatcatttg ctttagacta
    20221 tatatcttgg tgtacactca ctttaaggcg gaaataaaag ttatccgtaa taacaggttt
    20281 aggtatgaaa aaggagtggg tactatgtac aaggaattag aaaacaaagt cgcagtagta
    20341 actggtggct caaaggggat tggaacagcg attgctcaac gattcgggaa agaaaaaatg
    20401 aatgtagttg tcaactataa ctcggataaa gaaggagcag aaaaagcagt caaagcaatc
    20461 gaagaggcag gcggaaaagc agtctctgta caagcagatg tcagttctga agaaggaata
    20521 cagcgtttac ttgatacggc tttagaaaat tttgggacac tggacgtttg ggttaataat
    20581 gctgggatgg aaaatcagca tccaacccat gaacttagcc ttgaagattg ggaaagagtg
    20641 ttaaaagtca atttgactgg cgtttttatc ggaacgaaag cggctttaaa ttactttaca
    20701 gagaataata aaaaaggaaa tatcatcaat atgtcttctg ttcatgaaca gatcccatgg
    20761 ccaacgtttg ctcattacgc tgcttcaaaa ggcggggtaa aattatttac cgaaacggta
    20821 gcaatggagt atgcaccgaa gggaatcaga atcaatagta taggccctgg tgctatccgt
    20881 acacctatta atgcggcaaa atttgatgat cctgaacaaa aagaactctt ggaaagtatg
    20941 atcccactag gacgcgtagg tgaaccaaaa gaaatagcgg cgacagctgc atggttggct
    21001 tcagacgaat ccagttatgt tacaggtatt acactatttg cagatggtgg gatgacttta
    21061 tatccttctt tccaaggtgg aaaaggataa gggtgaataa aaatgggttt attgattgca
    21121 ttgatcccgg cagttggctg ggggatccag ccactgattt tgaaaaagat tggtggcaga
    21181 ccaacaaatg agatattagg aactggtatt ggagcggtgc ttgtcggctt actcgttcaa
    21241 ttattcgctt ctccagctgg tatttcagtg actacatttt tgattagttt actttctggt
    21301 gcattttggg ttattggaca aacaggacag tatacatctt tcaacttgat tggtgtttct
    21361 aaaacgatgc caatttcgac tgcattacag ttagtcggaa cctcgttgat tggtgtcttc
    21421 gcttttggag aatggtctgg aagtatgggg aaaatcattg gagcagcagc aatcattctt
    21481 cttgtatttg gttctgcttt aacggcaatc agtgatgatc ctggaaaaaa aggcggtatc
    21541 acaaaaggaa ttagtctatt agcatcaaca agtattggtt attgggtata cagtgcattg
    21601 cctaaactag ttgatgcaag cggactttcc attttctttc cgcaaatgat cggtgtcttt
    21661 ttaggggcag tgatttatgt gattttccgt aaaccaaccg cattttctga agacaagagt
    21721 tggaaagcaa cgatcgttgg agccatcttt agtgtgtcag cattggctta tattttttca
    21781 gcaaatgaaa atggggttgc tactgcatat atcattacac agctaaacgt agttatctct
    21841 acattaggcg gtatgtacat cctgcatgaa aataagacac caaaagaaaa aaaattaacg
    21901 attatcggat tattgctgat cgtagtagga agcatggtca cggtctttat ttgaggataa
    21961 agatggtaaa atttcattta ttaaaaaaat atttgtttga accaaacagt aagtacttat
    22021 aaaaaaatag cgctagaatt tccaacatct ctggaatttc tagcgctttt taattgttta
    22081 agatacaagg gaaatatctg tctggatcaa taatttttca ttgatccatt gactaaagtt
    22141 tacgacataa ttgaagttac caccgattag tatctccgca tgattgtctt tataaagatc
    22201 gtattgtaaa agacggtctt ttttatttgc gtaaacatcg ttatggtact ggactaattg
    22261 ttctcggaat tgattgattg ctgtaaaatc acgatattga aggttgaaac gcatcgtgat
    22321 tggtgcattt agcatgaatc gctgcatttc ttttgctgct tcctctgctt gatctccgta
    22381 taggaactca aaaagattac gttcagaaaa tttccattgc ccgccaacct ttgttccttt
    22441 tagttttcct tcatgaagat aatttctgat agaacgtgta gtaacaccta attgttcggc
    22501 taattcattt actgagtaaa aatctttcaa tgttcaccct cctatgtgaa tggttctact
    22561 gacttagttg atatttgctt aatcacgaaa tgtagaaaga acgattttaa ttaaggtcat
    22621 tgtaaaaatc aagtcagaaa attctaactt ttatttctct aaagaaattc gttctctcct
    22681 aaagtgatat tatatttaaa tatacctgaa aataatgggc agtattatga aagatcactg
    22741 atattttctc gtattttttc cttattcaaa agttttgcag gaaaattgct gaaaaaataa
    22801 ttttactatg tagaaaattc aaagccattc aatcagtgat ttttttcagc acaagaaaga
    22861 ctccttataa aaatagcaga tactttgtca attaagtgtt taaccaggga tgataaaaag
    22921 gataggattg aacaaaacct gtataaggtg atgaatagtt tcctcgccag taaaaggttt
    22981 attcgtataa agctttatcg ccaaaattta tcatgacctt ttttatcttc aatattaaat
    23041 gaaatcaatt gttcaatcaa ttcccagttg attacacttt tccaaggaac tttgatgacg
    23101 ttatccgtat atacgtagcc tgcttcttgg atattttttt caagggcacg aatcgctgct
    23161 ttttccggtg cgatagaaaa atgattttta gcaaaactaa atgcaataat aaaggtacca
    23221 tggtcagtaa acataggttg gttccatttt acagttgtgt ctagctgggg atacgtttct
    23281 gttatcttag caaaaaggtc tttgatcttt tgttgatgtt ctttttcttt gatttgattg
    23341 atgatcggct gaaatttttc cattctaaaa cgcctccatt aagtggtttg atttatttta
    23401 ccagaaaaac aatagttatg tataaaaaac gaacaaaaca caagtaatgt tgtaaccgct
    23461 atatttatat gataagatgt ctcaaataat aaaatagaaa agagacataa aaatgattga
    23521 cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc atcgagccat
    23581 ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag gtacagagac
    23641 caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga cttttactac
    23701 gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt ctattgtttc
    23761 atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc cgtttggtta
    23821 tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa aacttgtttt
    23881 tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa acaaaatcat
    23941 ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca attaaccaag
    24001 taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat tagaaagaaa
    24061 tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc attcgttact
    24121 cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg gaaagacgct
    24181 ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg aattcaatca
    24241 aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag aattattgat
    24301 tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag taggttattc
    24361 agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca ctgcacatgg
    24421 aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa tgtgggaaag
    24481 tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa agtatcaaaa
    24541 agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac caacaaattg
    24601 gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg gttgtattga
    24661 tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta aaagaaagta
    24721 taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt cagttgattt
    24781 aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct cggggatcat
    24841 gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg tatacgaaga
    24901 tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc atgtaccgcc
    24961 acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata gaaaagcgac
    25021 tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc aaagaccaat
    25081 aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct tctttttgta
    25141 taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt ttcacgtcat
    25201 tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct tgagttgttt
    25261 ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt gaaggaactg
    25321 gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc tggtaactgt
    25381 atttcccagt gagcataacc acatctttcg gatagactgc actccctgca tcataagggg
    25441 cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc gatgtatctc
    25501 taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt agatcagaat
    25561 atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct attgttgaag
    25621 aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc tctgagactt
    25681 tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta cttgattttt
    25741 cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga tcactatttt
    25801 ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa ataaaaccta
    25861 actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata atcctgaatt
    25921 tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt tgataaatgt
    25981 cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt gataaggagt
    26041 atttaattga agagaatgat taggagaaag gatctccgta ctttttatag taatcataga
    26101 atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca aaatggactg
    26161 cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta atcaaattac
    26221 gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt tccgattcta
    26281 accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg ctctgagcta
    26341 aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac aatttatcga
    26401 ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc ataataaaaa
    26461 ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt aatcctccaa
    26521 tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca ttttggttta
    26581 gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga aacgtaatcg
    26641 cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt tttaacctac
    26701 agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt tttagacaga
    26761 gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg taatcatttt
    26821 tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa ttcccacttg
    26881 cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa cgaaaaataa
    26941 atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag gtaaccagtt
    27001 taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg atttgttcgc
    27061 caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg tctgagcact
    27121 taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact taatgccgaa
    27181 ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta gctccgatga
    27241 caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg cttatttcaa
    27301 tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact tttcgacgta
    27361 aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg tttctgcgta
    27421 cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata aagttttttt
    27481 agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga aaataatgct
    27541 agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga caccaacttc
    27601 tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc tgccttttca
    27661 aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg aaaaacttca
    27721 agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat ttgataatcg
    27781 cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa ctatgcaatt
    27841 tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat taccgggaca
    27901 aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc tgcctcacta
    27961 ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg tacgacaagg
    28021 aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga cgattgaagc
    28081 aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct ccggacatga
    28141 atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg gagctggtgc
    28201 tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac actatttacg
    28261 cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac gaaaaaatca
    28321 aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta aaaaacattt
    28381 ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa gggaattaga
    28441 gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa gagggacctt
    28501 tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag aatatttgtt
    28561 ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat tgaaacgttg
    28621 atataactgg atttacatca attatttaga attagcactc ttgtgtttag agtgctaaaa
    28681 aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag tggattagca
    28741 cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg acaaaacgac
    28801 attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg ttccaaaaca
    28861 ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat gaaagcatta
    28921 gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc atctgcttta
    28981 ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga agatgatttg
    29041 ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat tattaagcaa
    29101 tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg acctgaaata
    29161 aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca gattattgct
    29221 atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc cgaatatatg
    29281 ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt cggagatcct
    29341 ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca taaatatttc
    29401 caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt ttttgaagat
    29461 aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca agatcttgat
    29521 cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca aatgatcact
    29581 ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga tttattgcag
    29641 aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg tacaatagcc
    29701 ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga tgtttatcga
    29761 agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc aaactcgtaa
    29821 tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag agcaatcaaa
    29881 cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga agaactagat
    29941 aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc agaagattcg
    30001 ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc tgagttagaa
    30061 gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc aaatcgagga
    30121 aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa aaaattactt
    30181 ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga tcaaggggca
    30241 ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa
    30301 ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca tcaagcagta
    30361 caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt actacaagaa
    30421 ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc acaataattg
    30481 tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg agtaaaatta
    30541 tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga aacgaagcaa
    30601 aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg
    30661 gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca aatacgattt
    30721 catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat
    30781 atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc gcagaagact
    30841 atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt aatgatgcac
    30901 aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa cgtatcgtca
    30961 atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc gacgaaaaaa
    31021 tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa ctaggagacg
    31081 gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac gactttgata
    31141 acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc
    31201 aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa aaagatttat
    31261 ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa tctggaccat
    31321 tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct gacttagtag
    31381 aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca caatcagaaa
    31441 tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta gaagcagtac
    31501 gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta gttgctatgg
    31561 gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg
    31621 ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa ttgattgacc
    31681 gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca gataatcaac
    31741 cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac aacaaaactc
    31801 taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca caaatcgaag
    31861 taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat ctaggtacac
    31921 aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa gaaatcgaac
    31981 gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa gaggaagttg
    32041 acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg aaagaattag
    32101 aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat gaattgaaag
    32161 ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca ttgaacgaaa
    32221 tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa gctcaagaaa
    32281 acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt gaagaagtcg
    32341 atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt tgctactttg
    32401 gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa aatatgatat
    32461 gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg gcaacaaaac
    32521 gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa ataaagaaag
    32581 cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct gatgcggaag
    32641 aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa aaacgcgctg
    32701 catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt ggagctggtg
    32761 gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt gaagatatct
    32821 tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg
    32881 ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt gtagaaaaag
    32941 aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt gcaaaaccag
    33001 gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat gtagaacgcc
    33061 aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat gggacaggga
    33121 aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag aaagcacatt
    33181 cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt ttagctggtc
    33241 aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc ttccgtgtag
    33301 aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt
    33361 ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat
    33421 tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga aaaggcgctc
    33481 cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg atcacaccga
    33541 aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct ggcggcatca
    33601 aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct tttggcggga
    33661 aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa aaaccagacg
    33721 aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa caaaaaagcc
    33781 agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag agaatggaac
    33841 ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga gcaatttttg
    33901 atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg tacttcttct
    33961 tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga cgggattgct
    34021 ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac agattgatgg
    34081 aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt ctcttgctct
    34141 gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt attaaacttt
    34201 gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt gactaggata
    34261 atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa gaccctttcg
    34321 agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc agaaagttgt
    34381 cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg gtggtatcga
    34441 ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg aacaggtaga
    34501 gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag gtgagaaaat
    34561 aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt tttattaaat
    34621 atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt caaagatttg
    34681 ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc gattggccac
    34741 ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt gttctgctaa
    34801 ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag aagtaggctc
    34861 atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca cacgctgttt
    34921 ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac caactgtctg
    34981 cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca ggtccatcgg
    35041 tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt gttggaatac
    35101 cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca agtgggcgcc
    35161 gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac gcaagaatgt
    35221 actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt tgatggatac
    35281 attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct caactaagat
    35341 tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt gtcaatgctg
    35401 tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt tgtgttgtac
    35461 gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg gaaatgatcg
    35521 ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg atgcgtatcg
    35581 cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa cttctggaag
    35641 cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct gcgatataag
    35701 cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc gtaaatccaa
    35761 cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga attcctcgga
    35821 tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct acactgaata
    35881 atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt aggagaattg
    35941 ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca agaaatgttg
    36001 attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca tattctttaa
    36061 taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg aacatattaa
    36121 gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca gattcttttt
    36181 tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc ggataatcat
    36241 ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca gcagcatcaa
    36301 aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca gcactttcgg
    36361 taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg atcgaagtat
    36421 ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa aaatcatagc
    36481 ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct tgtcctgatt
    36541 ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta aatccttgca
    36601 gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc tttgcgtttt
    36661 gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct tttttgggct
    36721 ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg tcagcttgtg
    36781 caacatgacc aaacgcaaat gtcacaagtc ctgtcattaa gaaaacaaat gagtgaaata
    36841 aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt ataggcgaaa
    36901 agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga gtttctaaca
    36961 ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa gtatgttcgg
    37021 tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta ttggtcgtaa
    37081 aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata cctcccgaca
    37141 ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga ttaaccaatt
    37201 agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga ctggaacagg
    37261 gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac tgattattgc
    37321 ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt tccctaacaa
    37381 tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt atgtgccttc
    37441 aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca aattacgaca
    37501 ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat ccgtttcatg
    37561 tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga ttcgacaggg
    37621 agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat tcgaaagaaa
    37681 tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg agatttttcc
    37741 ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg tcgaacgtat
    37801 ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg tagcaatctt
    37861 cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag cgaatatcaa
    37921 agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc tggaagctca
    37981 acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg gctacacgtc
    38041 gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac cgccttatac
    38101 gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc acgtaaccat
    38161 gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc tagtagatta
    38221 cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag agtttgaaaa
    38281 acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac atgaacaaac
    38341 agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag tgatcgaagt
    38401 tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac gagtcgaaaa
    38461 agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc tcacagatta
    38521 tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa cgctggaacg
    38581 aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg gaatcaattt
    38641 gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg atgcggataa
    38701 agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag ctgctcggaa
    38761 tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc gtctagcgat
    38821 ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac atgggatcgt
    38881 tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag aatcggaaga
    38941 tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag aaaaagacac
    39001 gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg attttgaaac
    39061 agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg aggacaacat
    39121 ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa atatagatgt
    39181 aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg gaaaaagttc
    39241 cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga gcctttctgc
    39301 ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca tcgacggatt
    39361 gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt cgacagtagg
    39421 tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag gacatccgat
    39481 ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg cagatcaagt
    39541 aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg taaagaaaaa
    39601 aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc gcgttcgagt
    39661 cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca aaaaacatga
    39721 tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc gcctatttga
    39781 ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg tgatcggtga
    39841 agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta ctgttggcga
    39901 actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag actgtgatgg
    39961 tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa cgaagacact
    40021 tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc ctcaaatgtt
    40081 agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag aattaccgga
    40141 agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc attatgaaaa
    40201 tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga agaatatcga
    40261 gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc tttatatgac
    40321 agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc tatctgttca
    40381 aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag cagtagattt
    40441 cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga ttttaaaaga
    40501 agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa ctttgagtcg
    40561 cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta cacaaattgg
    40621 atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat tgcatcaacg
    40681 cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca acaccctcgt
    40741 tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg acgtcggacc
    40801 tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag aagtagctaa
    40861 tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc ctgtaccaaa
    40921 agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag aaaacaatct
    40981 gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag gtgtgtctgg
    41041 ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc aaaaattaaa
    41101 taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa gtatcgaaaa
    41161 gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc cagcgactta
    41221 tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg caaaaatgcg
    41281 gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg aagcttgtcg
    41341 cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg ttccttgtga
    41401 agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca aaggaaaaag
    41461 cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca agcacattcc
    41521 aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg tgacgatggg
    41581 gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg ccagtgaatt
    41641 gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga caggacttca
    41701 tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg ctggtaacac
    41761 agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata tcattgattt
    41821 aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac cagaagaaat
    41881 cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg tataaagagg
    41941 atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg ttttattaat
    42001 atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta tagataaaaa
    42061 cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact ttcctttgat
    42121 tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa caagagatta
    42181 tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc cctatattat
    42241 taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca tacggcaaaa
    42301 tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa attgtcaatg
    42361 tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg cttcgtttgg
    42421 caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt ggtgatggat
    42481 atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc tgtccatata
    42541 ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa gtttgtgaag
    42601 aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta gtaaatggac
    42661 atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca aacagtgtgg
    42721 agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag actagagaag
    42781 agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa atgattgtac
    42841 aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat gtagatgaag
    42901 atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct acaccagcat
    42961 cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat caaaccatta
    43021 aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg aagtatgatc
    43081 ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt agcagtttct
    43141 ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga gtgttcaaca
    43201 aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga ttatggatgg
    43261 gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa aaactagctg
    43321 agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa gaccgatcaa
    43381 tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga ttcaaaaagt
    43441 attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta ggcggaatgg
    43501 gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa gctacaaaag
    43561 atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat cgtacagctt
    43621 atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat attgagaaac
    43681 aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac tacttttttg
    43741 aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa gcagcaaatg
    43801 aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa ggaagtatga
    43861 aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg attcctgata
    43921 ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa tcggattatt
    43981 tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta aactgtgaaa
    44041 aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa gataatcatt
    44101 acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt gcattagctg
    44161 aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt tggggcaaat
    44221 atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact ggtaatgata
    44281 cgcccgggat gatataattt tattatgcac aatgtgagag gggttcgtaa atatgacaga
    44341 taatttgcaa ttagtcatca taacaggtat gagtggtgca gggaaaacag tagctatcca
    44401 aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat tgattccaaa
    44461 attctgggaa ttgatcaaag aatcaggtaa agtcacaaaa atggctttag gagtggattt
    44521 gcgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg aaaatacgaa
    44581 cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag tggctcgcta
    44641 caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg aagggatacg
    44701 taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca ttgatacgac
    44761 aacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat caaagggaga
    44821 ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc cgatcgattc
    44881 ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg aactaagacc
    44941 attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg aaacgcaatc
    45001 attctaccat aaatttttgg atcttctgga aactgtattg cctggataca ttaaagaagg
    45061 aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt ctgtggcatt
    45121 gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga cacatcggga
    45181 caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta ccggataaga
    45241 aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct gaaaagttta
    45301 cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg tggaagcagc
    45361 ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa tgtcttagtg
    45421 gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt caaaaaggaa
    45481 gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt ttccgagatg
    45541 cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt agatggacat
    45601 gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tttttaaaga cggaacgact
    45661 gcagtaggag aatctaaaat cgcgatcaac cgtaaaacca ttgaccgtgt gtatgtctgc
    45721 aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc aattatggac
    45781 gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc aaatttagtg
    45841 ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta tatctgtaat
    45901 atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat acgtgtttta
    45961 cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga aaaagtgcct
    46021 gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc acacgacttc
    46081 aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttaga attgaaagat
    46141 ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt agttttcggt
    46201 tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc atttgctgca
    46261 gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa agcggaatta
    46321 gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt tattttgaac
    46381 gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa agatcattat
    46441 caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa caatgtctat
    46501 atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat catggatggt
    46561 atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa aatgcgttct
    46621 tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac gagtcgttat
    46681 catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga tatgttaaat
    46741 tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc gtatttaaaa
    46801 ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc aatgctccga
    46861 tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct agtcaattgt
    46921 gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga aaacatcgaa
    46981 ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga aatcgcagaa
    47041 cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat tccctcaggt
    47101 gcaatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt tgcggaaaaa
    47161 ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag gagatgcgaa
    47221 tgtatcagtt ttttttaaaa gctatacttg cttttcttcc gtcaaactat aattccaact
    47281 ttactggagt aacataatgt tgaaaggctt ctattgtttt ttaatacaat cgtataaaaa
    47341 gcggggagac aactgaatga agattttagt tttatacata aacttttgct taatatagaa
    47401 gtcctgatat agataggatc aagtatttat ctataactgg agattaatta aaaactggaa
    47461 ggaactgctc gatcaacctt gagaactgtt tttcttactt ctggtcaaga ttctcgtggt
    47521 tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca accaggtgta
    47581 attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa tcataagttc
    47641 ataaatagta attgggatgt atggttggta tacgttatac ctgaacttga actatcaaga
    47701 tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaaatg tgctttttta
    47761 caacttttac tatcattatg attgcagtac tttttatttt tctagatata gcaaagcgaa
    47821 atacagcatt tttattatat cgggttttac ttcgagcagg tctcataaca tttatatcga
    47881 tcgttggatt ttttctattc acagttattg tatttatttg gagaactaca gcaccaccat
    47941 tgccagagat tacatatgga gaatttccgt ttagtcttga atatgaaata gatgaggaaa
    48001 gaattttgat tgaagatacg gtgattgcag aatttatagg aagttttaga gggaatgtga
    48061 caactcaacc tagacgaagt tggagtataa aacttttaaa taatagtgga gagttgaaag
    48121 catttcatta tgaattctta ataatagaaa gtgaagatgt aacaatcact ttaagtagtg
    48181 gctctgctgg atattttatg aatgatggtg gaagcaagcc acagctcaca gtccgtgatc
    48241 gtattgcagg ttttatgcat ttttctcact atgacttaga agaagctcgt aaaatacttg
    48301 cagaatatgg gatcacatta atcaattggg aatatgcgcc accgataaga aattttttca
    48361 atgattatta ttagtcttat aaaaaacaga atgtaaaatt tatgtgcgcg agtatttagt
    48421 tttccatttt ttgtttgata gatatatagc attgttgaag catgggatag tggtgccaat
    48481 tttatcaatg aggttggtca ggcagttaac ataattggtg atgctataaa tccatttaat
    48541 tggaattgga ggaattaggt gtggaaacag tgagattatg gatattcatg aaaaattttc
    48601 gttcggcttt gagatatgtt attctggaat caaaatcagg ttattatctg attgacacag
    48661 atcactatac tttttgggga ggctaccttt ttccgatttt taattggata atcccccata
    48721 aggttatgaa aattaacttg cctgcagaag aaataaatag attaatactc aacaaagatt
    48781 tacaaaggaa aagagagaaa gaacattcta atgttgcttg gataggtcca gcatttgcag
    48841 tagtgacttc ttcaattgtc ggtcctatgc tagagagtat ttatagtgat ttttctattg
    48901 gaatacgtta tttatttgtt actttaagta tagctgttct tttgtgcata aagggttatg
    48961 ttagcaagaa gaataaagat attttaacac taatcggtgt aaacaattta tctagtacta
    49021 aaattatact gtttcctgaa tctataaaag aaggatcaag gttgttgata atttttataa
    49081 tgcttatact gttttctgtt ttatttgggg tgatgtttat a
//
LOCUS       DAYYJC010000011        45194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000011 DAYYJC010000000
VERSION     DAYYJC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.011"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..235
                     /locus_tag="ACHA9N_000594"
     CDS             35..235
                     /locus_tag="ACHA9N_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFM9084080.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            262..1455
                     /locus_tag="ACHA9N_000595"
     CDS             262..1455
                     /locus_tag="ACHA9N_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084081.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(1523..3088)
                     /gene="guaA"
                     /locus_tag="ACHA9N_000596"
     CDS             complement(1523..3088)
                     /gene="guaA"
                     /locus_tag="ACHA9N_000596"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9084082.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3147..4070)
                     /gene="coaA"
                     /locus_tag="ACHA9N_000597"
     CDS             complement(3147..4070)
                     /gene="coaA"
                     /locus_tag="ACHA9N_000597"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9084083.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            4266..4871
                     /locus_tag="ACHA9N_000598"
     CDS             4266..4871
                     /locus_tag="ACHA9N_000598"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9084084.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            5179..6195
                     /locus_tag="ACHA9N_000599"
     CDS             5179..6195
                     /locus_tag="ACHA9N_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084085.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            6336..7637
                     /locus_tag="ACHA9N_000600"
     CDS             6336..7637
                     /locus_tag="ACHA9N_000600"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9084086.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            7683..8345
                     /gene="deoC"
                     /locus_tag="ACHA9N_000601"
     CDS             7683..8345
                     /gene="deoC"
                     /locus_tag="ACHA9N_000601"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9084087.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            8366..8764
                     /locus_tag="ACHA9N_000602"
     CDS             8366..8764
                     /locus_tag="ACHA9N_000602"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9084088.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            8830..9912
                     /locus_tag="ACHA9N_000603"
     CDS             8830..9912
                     /locus_tag="ACHA9N_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9084089.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            10124..11686
                     /locus_tag="ACHA9N_000604"
     CDS             10124..11686
                     /locus_tag="ACHA9N_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084090.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            11679..12812
                     /locus_tag="ACHA9N_000605"
     CDS             11679..12812
                     /locus_tag="ACHA9N_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084091.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            12812..13768
                     /locus_tag="ACHA9N_000606"
     CDS             12812..13768
                     /locus_tag="ACHA9N_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084092.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            13867..15033
                     /gene="deoB"
                     /locus_tag="ACHA9N_000607"
     CDS             13867..15033
                     /gene="deoB"
                     /locus_tag="ACHA9N_000607"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9084093.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            15057..15875
                     /locus_tag="ACHA9N_000608"
     CDS             15057..15875
                     /locus_tag="ACHA9N_000608"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9084094.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            15892..16599
                     /gene="deoD"
                     /locus_tag="ACHA9N_000609"
     CDS             15892..16599
                     /gene="deoD"
                     /locus_tag="ACHA9N_000609"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9084095.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(16897..17583)
                     /gene="gpmA"
                     /locus_tag="ACHA9N_000610"
     CDS             complement(16897..17583)
                     /gene="gpmA"
                     /locus_tag="ACHA9N_000610"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9084096.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(17755..18336)
                     /locus_tag="ACHA9N_000611"
     CDS             complement(17755..18336)
                     /locus_tag="ACHA9N_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9084097.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(18469..19152)
                     /gene="rpiA"
                     /locus_tag="ACHA9N_000612"
     CDS             complement(18469..19152)
                     /gene="rpiA"
                     /locus_tag="ACHA9N_000612"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9084098.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            19471..19884
                     /gene="rpsL"
                     /locus_tag="ACHA9N_000613"
     CDS             19471..19884
                     /gene="rpsL"
                     /locus_tag="ACHA9N_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9084099.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            19968..20438
                     /gene="rpsG"
                     /locus_tag="ACHA9N_000614"
     CDS             19968..20438
                     /gene="rpsG"
                     /locus_tag="ACHA9N_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9084100.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            20532..22619
                     /gene="fusA"
                     /locus_tag="ACHA9N_000615"
     CDS             20532..22619
                     /gene="fusA"
                     /locus_tag="ACHA9N_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9084101.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            22791..23978
                     /gene="tuf"
                     /locus_tag="ACHA9N_000616"
     CDS             22791..23978
                     /gene="tuf"
                     /locus_tag="ACHA9N_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9084102.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            24385..24693
                     /gene="rpsJ"
                     /locus_tag="ACHA9N_000617"
     CDS             24385..24693
                     /gene="rpsJ"
                     /locus_tag="ACHA9N_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9084103.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            24724..25353
                     /gene="rplC"
                     /locus_tag="ACHA9N_000618"
     CDS             24724..25353
                     /gene="rplC"
                     /locus_tag="ACHA9N_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9084104.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            25381..26004
                     /gene="rplD"
                     /locus_tag="ACHA9N_000619"
     CDS             25381..26004
                     /gene="rplD"
                     /locus_tag="ACHA9N_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9084105.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            26004..26294
                     /gene="rplW"
                     /locus_tag="ACHA9N_000620"
     CDS             26004..26294
                     /gene="rplW"
                     /locus_tag="ACHA9N_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9084106.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            26333..27166
                     /gene="rplB"
                     /locus_tag="ACHA9N_000621"
     CDS             26333..27166
                     /gene="rplB"
                     /locus_tag="ACHA9N_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9084107.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            27209..27487
                     /gene="rpsS"
                     /locus_tag="ACHA9N_000622"
     CDS             27209..27487
                     /gene="rpsS"
                     /locus_tag="ACHA9N_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9084108.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            27510..27857
                     /gene="rplV"
                     /locus_tag="ACHA9N_000623"
     CDS             27510..27857
                     /gene="rplV"
                     /locus_tag="ACHA9N_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9084109.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            27871..28527
                     /gene="rpsC"
                     /locus_tag="ACHA9N_000624"
     CDS             27871..28527
                     /gene="rpsC"
                     /locus_tag="ACHA9N_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9084110.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            28530..28964
                     /gene="rplP"
                     /locus_tag="ACHA9N_000625"
     CDS             28530..28964
                     /gene="rplP"
                     /locus_tag="ACHA9N_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9084111.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            28954..29142
                     /gene="rpmC"
                     /locus_tag="ACHA9N_000626"
     CDS             28954..29142
                     /gene="rpmC"
                     /locus_tag="ACHA9N_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9084112.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            29167..29433
                     /gene="rpsQ"
                     /locus_tag="ACHA9N_000627"
     CDS             29167..29433
                     /gene="rpsQ"
                     /locus_tag="ACHA9N_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9084113.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            29491..29859
                     /gene="rplN"
                     /locus_tag="ACHA9N_000628"
     CDS             29491..29859
                     /gene="rplN"
                     /locus_tag="ACHA9N_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9084114.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            29896..30204
                     /gene="rplX"
                     /locus_tag="ACHA9N_000629"
     CDS             29896..30204
                     /gene="rplX"
                     /locus_tag="ACHA9N_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9084115.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            30232..30771
                     /gene="rplE"
                     /locus_tag="ACHA9N_000630"
     CDS             30232..30771
                     /gene="rplE"
                     /locus_tag="ACHA9N_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9084116.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            30790..30975
                     /locus_tag="ACHA9N_000631"
     CDS             30790..30975
                     /locus_tag="ACHA9N_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9084117.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            31011..31409
                     /gene="rpsH"
                     /locus_tag="ACHA9N_000632"
     CDS             31011..31409
                     /gene="rpsH"
                     /locus_tag="ACHA9N_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9084118.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            31441..31977
                     /gene="rplF"
                     /locus_tag="ACHA9N_000633"
     CDS             31441..31977
                     /gene="rplF"
                     /locus_tag="ACHA9N_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9084119.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            32138..32494
                     /gene="rplR"
                     /locus_tag="ACHA9N_000634"
     CDS             32138..32494
                     /gene="rplR"
                     /locus_tag="ACHA9N_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9084120.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            32515..33015
                     /gene="rpsE"
                     /locus_tag="ACHA9N_000635"
     CDS             32515..33015
                     /gene="rpsE"
                     /locus_tag="ACHA9N_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9084121.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            33030..33209
                     /gene="rpmD"
                     /locus_tag="ACHA9N_000636"
     CDS             33030..33209
                     /gene="rpmD"
                     /locus_tag="ACHA9N_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9084122.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            33253..33693
                     /gene="rplO"
                     /locus_tag="ACHA9N_000637"
     CDS             33253..33693
                     /gene="rplO"
                     /locus_tag="ACHA9N_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9084123.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            33693..34988
                     /gene="secY"
                     /locus_tag="ACHA9N_000638"
     CDS             33693..34988
                     /gene="secY"
                     /locus_tag="ACHA9N_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9084124.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            35050..35697
                     /locus_tag="ACHA9N_000639"
     CDS             35050..35697
                     /locus_tag="ACHA9N_000639"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9084125.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            35888..36106
                     /gene="infA"
                     /locus_tag="ACHA9N_000640"
     CDS             35888..36106
                     /gene="infA"
                     /locus_tag="ACHA9N_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9084126.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            36139..36255
                     /gene="rpmJ"
                     /locus_tag="ACHA9N_000641"
     CDS             36139..36255
                     /gene="rpmJ"
                     /locus_tag="ACHA9N_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9084127.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            36273..36638
                     /gene="rpsM"
                     /locus_tag="ACHA9N_000642"
     CDS             36273..36638
                     /gene="rpsM"
                     /locus_tag="ACHA9N_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9084128.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            36666..37055
                     /gene="rpsK"
                     /locus_tag="ACHA9N_000643"
     CDS             36666..37055
                     /gene="rpsK"
                     /locus_tag="ACHA9N_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9084129.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            37135..38073
                     /locus_tag="ACHA9N_000644"
     CDS             37135..38073
                     /locus_tag="ACHA9N_000644"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9084130.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            38101..38481
                     /gene="rplQ"
                     /locus_tag="ACHA9N_000645"
     CDS             38101..38481
                     /gene="rplQ"
                     /locus_tag="ACHA9N_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9084131.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            38753..39592
                     /locus_tag="ACHA9N_000646"
     CDS             38753..39592
                     /locus_tag="ACHA9N_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9084132.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            39568..40437
                     /locus_tag="ACHA9N_000647"
     CDS             39568..40437
                     /locus_tag="ACHA9N_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9084133.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            40427..41224
                     /locus_tag="ACHA9N_000648"
     CDS             40427..41224
                     /locus_tag="ACHA9N_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9084134.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            41427..42173
                     /gene="truA"
                     /locus_tag="ACHA9N_000649"
     CDS             41427..42173
                     /gene="truA"
                     /locus_tag="ACHA9N_000649"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9084135.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(42506..45175)
                     /locus_tag="ACHA9N_000650"
     CDS             complement(42506..45175)
                     /locus_tag="ACHA9N_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9084136.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14566 a         8347 c         9747 g        12534 t
ORIGIN      
        1 gttttcctag cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt
       61 agttgagcta ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt
      121 gatgattaca aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga
      181 agctgttgaa gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt
      241 gcacagtaag gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc
      301 tattattttc gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt
      361 agtggattca ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa
      421 ccagaagaat taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt
      481 ttccttccat ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact
      541 attaagaagc atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat
      601 gtgcaaaatt ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt
      661 caaggactat tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat
      721 ccatctaaga ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa
      781 gaagaatttg aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta
      841 tttatctctc tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt
      901 caatgggaag atattgattt tgactcagga gtactaacca tcaacaaaac actctattac
      961 aagaatcaat cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt
     1021 gtattagata aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta
     1081 attcagacag actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca
     1141 tatgctattg aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta
     1201 cggcattctc atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat
     1261 cgtctaggac acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac
     1321 agtaatttcg aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa
     1381 aattatgata cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg
     1441 aaaaaagtgc aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag
     1501 aggatttcta gctctcctac aactattccc actccactgt tgctggtggt ttagaagtga
     1561 tgtcatacac gatacggttg acgtgggcta cttcgtttac gatacgtaca gagatttttt
     1621 gcaagacttc ccatgggata cgggcaaagt cagcggtcat tccgtcgatt gaagttacag
     1681 cacggatacc gactgtgtag tcgtaagtac gtccgtcacc cataacaccg acagagcgga
     1741 tccctggaag aacagtgaaa tattgccaga tatcacggtc aagtccagca gctgcgattt
     1801 cttcacgcaa gatagcatca gattcgcgta cgatttctag cttgtcttca gtgatttcgc
     1861 caagaacacg aataccaaga ccaggaccag ggaatggctg acgccaaacg attgaatcag
     1921 gcatgcctaa ctgagtccct aattcacgga cttcgtcttt gaataatgtg tttaatggtt
     1981 cgatcaattc aaattgcata tcttctggca gtccacctac attatggtga gatttgatcg
     2041 tctgtgctgt ttctgtacca gattcgataa cgtcagtata caacgttcct tgtgctagga
     2101 aagagacgcc ttcttcacca gcaagttttg ttgcttcgtc atcaaaaaca tatacgaatt
     2161 cattcccaat gattttacgt ttttgttctg ggtcagatac acctttcaac ttgtctaaga
     2221 agcgatctct tgcatcgact ttgatgatgt tcaagccgaa ttttccgcct agactttcca
     2281 ttacttgttc cgcttcgcct ttgcgtagca gaccgtggtc aacaaagata gatgtcaatt
     2341 ggtcaccgat cgctttttgt aaaagaaccc caacgacact agaatcaacg ccacctgaaa
     2401 gaccaagcaa gacttttttg tcaccaactt gttcacggat tttttctact tgcatatcga
     2461 taaagttatc cattgtccag ttaccttcac agtgacaaac atcaaaggca aagtggcgca
     2521 gcaattcgat tccgtattcg gaatgacgta cttctgcatg gaattggata ccataaaaat
     2581 tacgtgttgt gtcttggata gaagcaatcg gacaatcttt gcttgttccg actgtttcaa
     2641 agccttcagg aactttagtt accaagtcac catggctcat ccatactgtt tgttgttttg
     2701 gtgtttcttt aaacaagaca gcttgatcgc ttgttacttc taacaaggct ttcccatatt
     2761 cgcggttttt agcaggctct acggaaccgc caagtttgta cgtgatcaat tgcataccat
     2821 agcaaatccc taatacagga atacctaaat tgaagatctc aggatcgatc ccaaatgcat
     2881 tctcatcata tacactgttt ggtccgccag aaagaatgat tccctttgga ttcatcgctt
     2941 ttacttcttc tgcagtaatg cggtggctca ataattcgga aaagactcca agatcacgaa
     3001 tacgtcttgt gatcaattgg ttatactgac taccgtagtc taatacgata atcttttcaa
     3061 cgttcgtcat atcggcaacg tttgtcaaat ttattacccc tatcccttta aattttatct
     3121 caaaatagtc aaactatttt aaaatgctaa tattttcgca tgaaaatcga atcgatgata
     3181 tgattttcag ttttatggag aattatatcg gctcttcctc gtgtaggcaa gatatattcc
     3241 tgtaaatttt tcaaattcac atttttccat acatcttttg ccattttaaa ggcttcttca
     3301 cgatctccga tagcaaaacg ataataataa ttctctggat tttggaaagc cgtatccagt
     3361 aacgcaccaa aacgatccaa ataccatttt tcgatcagca cgggatcggc atcgacatag
     3421 acagaaaaat caaaaaagtc gctgacgtag atttgctgat tcgcaggtaa ttgaagcgta
     3481 ttgatcccct caacgatcaa gatatcaggc tgctggatca attcgtactt gtctggaatg
     3541 atatcataca catcatgtga gtaaacaggt acttttatct cttcctgacc agattttaca
     3601 cggttcaaga aatcgatcag catctccata tcataacttt caggaaatcc tttacggtcc
     3661 atgattccac gctcttttaa tatacgattt ggataaagaa atccgtcagt cgtgatcagc
     3721 tgaacgtttc ttcgtttgaa ggtacgagac aaaatcgtct gcagcagtct ggctgttgta
     3781 ctttttccaa cggctacact tccggcgatc ccgataataa aaggcggtac ttctacgtat
     3841 ttatgaagaa ataaaccttt actcaagaac aaagattcaa attctttcat ataaagataa
     3901 atcagatgag caagaggtac atagatatct cgaacatcct gcatggaaat gcggtcattc
     3961 aaacttttga tattatctag ttcatcttgt gtcaaaggtg cttgcccatt acgataaaat
     4021 tctcgccatt cttctcttgc cacacggtag tagttcattg gatcattcat attagcctcc
     4081 tgcgttctcc catcaatgga actattttaa cacaaaataa acgcaaaccc caccacttaa
     4141 gagagattgc cgatcttttt gtataatctt tataatagaa agaaacggag tcttattctt
     4201 gacttgcgca cggatacata aaaggataga ctgttgtcat gaaagaaatg agaaggatgg
     4261 aagacatgac aaatcattac tattctgaac gcccagatac ggcgcacgat tttgaacaat
     4321 gggcttttga gttaaaaggc agaacctttc aatttgtaac agattctggt gtattttcac
     4381 gtgaaacagt ggattatggt tcacgtgtcc tgattgatgc tttcgattgg gaagagttac
     4441 ctgatgaagg aaagatctta gatgtgggat gcgggtatgg accgattggg ttagctttgg
     4501 catttgcgac acagcggttt gttgaaatgg tcgacattaa tgaacgagcg gtaagtctag
     4561 cgcaaggaaa cgcaaagcgt aaccaaatcg aacatgttga tatccatcaa tcgaatatct
     4621 atgaagcagt tcatgaagaa acctatgcag ctatcgtaag caatccgccg atccgtgctg
     4681 gaaaaaaagt tgtacatgaa attttgacag gcgcatatcc tcggttgaag aagggcggta
     4741 cactgacaat tgtgatccag aagaaacaag gagcgccgag tgcgcaaaag aaaatggaag
     4801 aaacatttgg taatgcagaa atcgtcacaa aagataaagg atattatatc atcaaaagtg
     4861 tgaaagaata actagctgaa aaatagtgga gcgacgaaag agtggaactg ggcgtgcatg
     4921 tacgggtacg tatcagttct ttttttgttg agaagttttt aaaaacaaag aactattttt
     4981 tcttcaaaaa gaaatagtag gaacagagtc gttgtttttt acgataaaat aaataagata
     5041 cagaacttgt atagaaggct gccaagtcgg tagataaagg aggtttttat caaaaaacat
     5101 actttccttt tttctctatt cttttcttgg gggttttggt acactgatag ggaatttaga
     5161 tagaagggag ccaataggat gaagctgacc atcagagata tcgcagaaat ggcaggcgta
     5221 tcagtaacaa ctgtctcaca gattttgaat aacaaaggaa gcagatttag tgataagaca
     5281 agaaaaaaag tgttgtcgat cgtgaatgaa catcattata agcccgatta tttcgcttca
     5341 aatgtgatca atcgacattc aaagaccatc ggaatgatcg tccctgatgt cactgatttc
     5401 ttcttctcca aactgatcga aggtgtagag agctatttga atcctttggg ctatgtgatc
     5461 atgttatgca attctcgtca tagccaagaa aatgagatca agtatttaca agagctttcc
     5521 caccgctcag tggacggaat cttgctggcc acaccaaata ttttacctga gaaatatgca
     5581 ttagacagcg gtttttatca aaaaatgcca atcattttaa tcgatcgcgg attgaatcgt
     5641 cgggataacg gacggcttat cgtaaaggaa tatgagggcg cctatcaagc tgtttcgctg
     5701 tttattgaaa atggccatac gaagatcggc atgctgaagg aaaccactgg atactatcaa
     5761 ttagaagaac gtttcaacgg atatcgtcat gcgctgaaag atcatggcct gccttttaat
     5821 gggaaatttg ttgaacatgg agagttgact gtccaaggtg gatatactgc atcaaaaaaa
     5881 ttactcaaac ataaagatat cacggctatt ttttgcggga atgatgccat ggcgatcgga
     5941 tgctatcagg cgatcgatga attgggcaaa aaaatcccag aagatatctc agtcatagga
     6001 ttcgatggat taaagctgtc agaatacatg attccgcaat tgacgacagt gcagcagcca
     6061 agttttgata ttggatttta tgctgcacgt ttcctgatcg atacgatcga atttcctcaa
     6121 agaaaagttc cgaacaaagt gttcgaaaca aaattaatta ttcgtgagag cgtaaaagta
     6181 ttgacaaagg gctaactcgg cggtatattg cttatgtaag catttacaaa ggctagttag
     6241 tgcttttttt atttttagta aaccagttta ctaaagtggt ttacctttcc taaaaataat
     6301 gtataatacc gaatgaagac aaggaggatc aggcaatgcg aatggtggat ctgattgaaa
     6361 aaaagaggga tggaaacgag ctgtcgaaag aggaaatcga gtacatcgtc acgaactata
     6421 cgaatggaaa gattccagat tatcaagtca gtgcattatt gatggcaatt ttttatcaag
     6481 acatgacaaa tgaagaaatc acgaacttaa cattggcgat tgctaattct ggtgatgtca
     6541 tcgatttgag ttctcttgaa ggaataaagg tagataagca ttcgacaggt ggcgttggtg
     6601 acacgaccac attgatttta gcccccctag tagctagtgt cggagtgaca gtggctaaaa
     6661 tgtctggacg cggtctaggt tacacaggtg gtacactaga caagctagaa gcaattcccg
     6721 gatttcagat cgagctttcg gatgaagaat ttgtacgcat tgttaatgaa agtaaagtag
     6781 cggtaattgg tcaatcagga aacttggcac cggcggacaa aaaattgtat gccttgcgtg
     6841 acgtcacagc gaccgttgat tctcttccgc tgattgccag ttcgattatg agtaaaaaaa
     6901 tcgcggcagg agcagatgca atcgttttgg atgtgaccac tggagacggt gctttcatga
     6961 agaacatcga agatgcaaga cgtctggcaa agaccatgac gagcattggg aaactggcta
     7021 accgagaaac agtggcggtt atctcagata tgtcagaacc tcttggcgaa gctatcggaa
     7081 atagtctaga agttgtcgag gcaatcgaaa cattacaagg aaatggtcca gaagatctag
     7141 tagaaatgtg ctacgcctta ggaagccaaa tggttgttct tgctggcaaa gctaagacaa
     7201 ccgatgaagc aagaacgttg cttcaagaag ctctggaatc aggtaaagcg ttagcaaaat
     7261 tcaaagaaat gatccaaaat caagggggag acccagcgat cgtggaacat ccagaacgaa
     7321 ttttgactgc acgctacaca atggagcttc ctgctaaaca aagcggagtt gtgtcaaaaa
     7381 tcgtggcaaa tgaattagga attgctgcaa tgatgctagg tgctgggcgt aaaaccaaag
     7441 aagaagatat tgatcacgct gttggattga aactgcataa aaaaattggt gacactgtga
     7501 caaaaggtga gtcactattg acgatctata gtaacgacga agaaatcact tctgtcattg
     7561 aattattata taaaaatatt gaaattggtg aatcggcaat gaagccaacg ttgattcatg
     7621 acatcatcac agaataaagg aaaaaagcgg actttaccgt tttaaagaaa aggggaaaag
     7681 aaatggaact aaaccgaatg atcgaccata ctattttgaa agcagacgca tctaaagaag
     7741 ctgtgatgca aattatcgaa gaagcaaaaa aataccattt ttattctgtc tgcatcaatc
     7801 cagcatgggt tgcgctagcg aaagaacaat tacaaggtga gccagtagcc gtatgtacag
     7861 taatcgggtt tcctctagga gcaaacacac ccgagacaaa agcatttgaa acaacagatg
     7921 ccatcaataa cggtgcagat gaagtcgata tggtcatcaa tatcggtgct ttgaaatcaa
     7981 aggatgatca acaagtccaa aaagatatcg aagcagttgt tgaagcagca aaagatcgtg
     8041 cgcttgtcaa agttattatt gaaacatcgt tattagatag agaagaaatc atccgagctt
     8101 gcgaaatagc aaaagctgca ggcgctgatt ttgtcaaaac atcaactggt ttctcaacag
     8161 gcggagcaaa ggtagaggat gtccgtttga tgcgtgaaac tgtaggacca gaaatgggtg
     8221 tcaaagcttc cggcggtatc cataacgaag aagaagcaat ggcaatgatc gaagctggtg
     8281 ctacaagaat cggaacaagc gcaggagtag caatcgtatc tggttcaact ggtgaaggct
     8341 attaattttt tcaaaggaga agctcatggc agaactcaaa caagaatgga tcaacacagc
     8401 tattgaagcg ttagataaag cctatgttcc ttattcccat tttccggtag gcgcttgtct
     8461 cgtaacggaa tcaggcaaga tttatcaagg catcaacatt gagaatgcat catttggatt
     8521 aacgaattgt gccgagcgca cagctttttt caaagcagta tctgaaggag aaaaaacatt
     8581 tacgcatcta gtcgtagcgg ggcacacccc cgatcctatc tctccttgtg gtgcatgtcg
     8641 acaagtaatg gcagaattct gttcccctga tatgccggtt acgctagtag gagacaatgg
     8701 tgtgacgaag gcaacgaccg ttcgtgcact gctgccttat gcatttactg aaaaagatct
     8761 ataactcatg ttcattcagt cagctgactg ttgaaaaata aaacaattat tttaggaggc
     8821 tttacagaaa tgaaaaaagc aaaattattt ggtttaggtg ctgtcgcact agcagcaggg
     8881 ttatttttag gtgcatgcgg caacaacggc tctactgatt catccggtgg caaaagctca
     8941 tcagacacaa caactgcagc attgatcaca gacacaggtg gtgtagacga ccgttcattc
     9001 aaccagtcag cttgggaagg actagaaaaa tgggggaaag atcaaggact ttcacgagga
     9061 aatgatggct tccaatattt ccaatcttca aatgaatcag attacatccc aaatattgac
     9121 caagcattga atgctggatt caaaacgatt tttggtattg gttataaact aaaaccagcg
     9181 atccaagaac aagcaacaaa caatacaggt acgaactttg ttattatcga tgatgtcatc
     9241 gatggattag ataacgttgt atctgcaact ttcaaagaca acgaagcttc ttatcttgca
     9301 ggtgttgcag cagcttatac gactgaaaca aacgtagtcg gctttatcgg cggtgtaaaa
     9361 ggtgaagtaa tcgatcgttt tgatgctgga ttcaaagccg gtgttgatgc aggtgcgaaa
     9421 gaattaggaa aagaaatcaa agtattgaat caatatgctg gtgacttctc tgctccagat
     9481 aaaggacgtt caattgctca aggtatgtat gcacaaaacg cagatatcat cttccatgct
     9541 tcaggcggta caggtaacgg tgtattccaa gaagcaaaat cattgaacga atcaggcgac
     9601 aaaaaagttt gggttatcgg tgttgaccgc gaccaatcag acgaaggcga atacacatta
     9661 aacggcgaaa agaaaaactt cacattgaca tctacactga aagcagttgg aacagtagta
     9721 gaagatttgg cacaaaaatc agcggacggt aaattccctg gcggcgaaca cactgtttat
     9781 ggattgaaag aagacggtgt tggtctaaca gaaggacaac tttctgacga agcgaaaaaa
     9841 gcagttgatg aagcaaaaga aaaaatcatt tcaggtgacg taaaagttcc agaaactcct
     9901 gaagaaaact aaaaccattt ctatagcctt gttagacaca tgtttgacaa ggctatggtt
     9961 tgattagaac aagaattgtt catttatcca agctgccttg tgacagtttg gataagggaa
    10021 caactccctg aagtaatgtt tgtttcaaaa gaagaacgct cactaagcgt gaatcttagt
    10081 caatcgttgc cctagataga aatcgttagg atgtgaaaaa aaggtggttc aagaaaatat
    10141 tgtcatagaa atgcagaata ttaccaaaga atttggaaca ttcaaggcaa atgacaacat
    10201 caatttacaa gtaaaggcag gagaaattca tgcgttgctt ggagaaaacg gtgctggcaa
    10261 atctacattg atgaacgtgc tttccggatt attagagccg acatcaggga aaattttgat
    10321 gcgtgggaaa gaagtaaaga tcacaagtcc gaccaaagcc aaccaattag ggattgggat
    10381 ggtccatcag cactttatgc ttgttgatgc ctttactgta acagaaaaca tcgtgttggg
    10441 aagcgagcct agtcgcgcag ggatgcttga ccataaaaaa gcacgaaaag agatccaaaa
    10501 agtttctgaa cagtatggat tgtcagtcaa cccagatgct tacgttcgtg atatttcagt
    10561 tggaatggaa caacgggtag aaattttaaa aacactttac cgaggagcag atgtactgat
    10621 ttttgatgag ccgacagctg tattgacccc tcaagaaatt gatgaattaa tcgtgatcat
    10681 gaaggaatta gtcaaagaag gcaagtcgat cattttgatt acgcataagt tagatgaaat
    10741 caaagcagta gctgaccgct gtacagttat ccgccgtgga aaaggaatcg gtacagtcaa
    10801 cgttaaagac gtaacctcac aacaattagc cgatatgatg gttggaagaa cggtttcatt
    10861 caaaacgatg aaaaaagaag cgaagcctca agaagtcgtt ttgtctattg aaaatctagt
    10921 ggtgaaagaa aatcgtggat tagaagccgt gaaaaacttg aacttagagg ttcgtgctgg
    10981 cgaagtactt ggtatcgctg gaatcgatgg aaacgggcag tcggagttga tccaagcttt
    11041 gactggtttg cgaaaggcag aaagcggaca tatcaagcta aaaggggaag acatcactaa
    11101 taaaaaaccc cgaaaaatca ctgaacatgg tgtaggacac gtgccagaag accgtcataa
    11161 atacgggtta gtcctagata tgacattatc tgaaaatatc gcactacaaa cctatcatca
    11221 aaaaccttac agtaaaaacg gcatgctgaa ttattcagta atgaatgaac atgccagaga
    11281 attgatcgaa gaatatgatg ttcgaacaac gaatgaactt gttcccgcaa aagctttatc
    11341 aggcggaaat cagcaaaaag caattatcgc tcgtgaagtc gaccgagatc ctgatctgtt
    11401 gattgttgca aatccaactc gtggactgga tgtaggtgcg atcgaattta ttcataaacg
    11461 tctgatcgaa caaagggaca aatacaaagc agtgttactg atcagtttcg aattagaaga
    11521 aattttaaat gtttcggatc gtattgctgt tatccatgaa ggagaaattg tcggtatcgt
    11581 tgatccgaaa gaaacatctg aaaatgaatt aggtctattg atggcgggct ataccttaga
    11641 agaagcacga aaagagttag aaaagggggc tggcgcacat gaatgatcgc agcgaaaaat
    11701 tccgtaattt attagtgcct gtactttcag ttgttttagg attgatctta ggcgcgatat
    11761 tgatggctgc ttttggtttt cagcccatcc aaggttattc ttctatgcta gatgcagccc
    11821 ttggttcgca aagagggata ggtgaaacgt taaggcaagc aacgccgttg atttttacag
    11881 cattaggttt ttctgtagct aactcagctg ggtttttcaa tattgggctt tctggacaag
    11941 cactttgcgg gtggatcgtc agtgtctggg tggcacttgc gtttccagat ctgccaaaag
    12001 ttgttttgct tccgttatgc gtgatcttag gtgccttagc tggtgcgatg gcagcggctg
    12061 ttccaggttt acttcgagca ttttttggaa caagtgaagt gatcgtaaca atcatgatga
    12121 actatatctt attatacgta agcacatttg ctttacatga agtgatgcct gaaagctatc
    12181 gctcaagttt ggactcttcc aatatgatca gtcaaaatgc ctcactgcgg ttaccatggt
    12241 taacacaagc ttttggcggc tctcgcgtaa actcaggatt atttttagca atcatcgcgc
    12301 ttattttcgt ttggctgctg atgaagaaaa cgactttagg atttgaaatc cgttctgttg
    12361 gattgaatcc ttttgcttct gagtatgcag gaatgagtag taaacgtacg atcgttttgt
    12421 cgatggtcat ttctggcgct ttggcagggc ttggcggtgt ggttgaagga ttaggaacgt
    12481 atcaaaactt ctttgttcaa acgacttcgc tatctattgg atttgacgga atggctgttt
    12541 ccttgcttgg ttctggatca gcaatcggta ttcttttatc agcgatcttg ttcagtattt
    12601 tgaagatcgg tggacttggc atgcagacag gagcaggtgt tccgtttgag atcgtcaatg
    12661 tatcgattgc tttgattatc ttctttgtcg ggatcaactt cctgattcgt ttcgtcatgg
    12721 ctaaagtctt ccaagggaaa aaacaagaag agatcgttgc gacaatagaa tcgaaaccaa
    12781 cgaatcaaaa ccatgaaggg ggcgaactat aatgtcgact atcgaattaa ttgccttgat
    12841 ccttacttct acactggttt attcaacacc gctaattcta acgtcactag gcggaacttt
    12901 ttctgaacga agcgggatcg taaacgtagg gctagaggga atcatggtca tgggtgcttt
    12961 tagttccatc gttttcaact tgacgatgag tccatcactt ggttctatga caccatggat
    13021 cgctatttta gtgggtggcg tcgtaggtat cctattttct ctgattcacg cagtagcaac
    13081 agtcagtttg cgtgctgacc atattatcag cgggacagta attaatctga tggcacctgc
    13141 tttaggtgtt ttcttgatca aagcatggta tggtaaaggt caaactgata acatcacgca
    13201 gaatctgggg tatttttctt tcccaggatt agcagatatt ccggtcattg gaccaatttt
    13261 ctttaaaaat acattcttac ctgcttattg tgctattttg attgctgtcc tagcttgggt
    13321 gatcatcttc aagacgaaat tcggcttgcg cctccgttca gttggtgaaa atcctcaagc
    13381 tgccgacaca ttaggtatca atgtatatgc tatgcgttat tcaggcgtac tgatttctgg
    13441 cttgttaggc ggtatgggcg gtgctgtttt tgctcagtcg atttccggta acttttcagc
    13501 tggtacgatc gttggacaag ggttcatctc catggctgct atgatctttg gtaagtggaa
    13561 tcctttaggg gcaatgggtg cctcattgtt ctttggtttt gctcaaagct tgagtatcat
    13621 tggcgcacaa ttaccagtta tttcttcgat tccgccagta ttgctgcaaa ttgctccata
    13681 tttgctgaca atcattgtgc tggttgtttt cttagggaaa gcttctggtc caaaagcaaa
    13741 cgggaagaac tatatcaaat caaaataatc ttttagcgaa taaaaagggt gggttacggg
    13801 aagtgttttt cgtctcgccc tttttgtttt aaaggcaata aaaataaaaa gaaagaagga
    13861 ataatcatgt ttaaacgcgt acatctgatc gtcatggatt cagtaggaat tggagaagca
    13921 ccagatgccg ataagttcgg tgatgttggt tctgatacat taggacacat tgcgaaagaa
    13981 gcaggattga acgttccaca tttagagcag cttggtttag gaacgatccg tcctcttgaa
    14041 ggtgtggaat cagtgaaaga tcatcaaggc tatgcgacga aattagaaga agtttctgta
    14101 gggaaagata cgatgacagg ccactgggaa atcatgggat tgaatatcaa aactccattt
    14161 cgtgtgtttc ctaatggatt ccctgatgat ctgatcaaac aaatcgaaga attttctggc
    14221 cgtaaagtcg tatgtaacaa accttatagc ggaacagctg ttattgacga ttacggtgaa
    14281 caccaaatga aaacaggcga tttaattgta tatacttctg ccgatcctgt ccttcagatc
    14341 gcggcacatg aagaaatcat tcctttagaa gaattatatc ggatttgcca attcgttcgt
    14401 gatataacaa aagatgatcc gtatatgatt ggccgaatca ttgcacgtcc gtacgtgggt
    14461 gaaccaggag acttcacacg aacaagcaat cgccatgatt atgcgttgaa tccattcgga
    14521 cacacagtcc ttgattcttt gaaagaagca ggaaaggacg tgattgctgt tggaaagatc
    14581 aacgatattt tcaacggaca aggaatcact gaatctgtcc gcacgaaaag caatatggat
    14641 ggcgttgacc agctgctgaa agttatgaaa aacgattttt caggaatcag ctttaccaat
    14701 ttagtagatt ttgatgcatt atacggtcat cgtcgggatg tagtcggcta tgcgcatgcg
    14761 attgaagaat ttgacttacg aattccagaa ttgcttgaag cgatggaaga agacgacttg
    14821 ctaatgatta cagccgatca cggaaacgat ccgacgttta ctggtaccga tcatacgcga
    14881 gaatatgtac cgttattggt ttacagtaaa aaaatgaaag aacaaggggc attgcctcaa
    14941 ggatattatt cagatatttc agcaacgatt gctgagaact ttagtgtttc ttcgactgaa
    15001 aatggcgaaa gcttcttgaa attattaaaa taatcttttt aaataggaga aagcaaatga
    15061 ccaagttaag cgagatgtta caagaaacga cccagtttat ccttgataaa ggagtaaaag
    15121 aaattgattt tggtttgatt ctaggttcag gattaggtga attagcagat gaaatcgaag
    15181 atgctgtaat gatttcttat gatgagatcc cgttctttcc tgtttcgaca gtagttgggc
    15241 atgcaggaaa gctagtttat gggtctctgg caggcaagaa agtgttagcc atgcaaggga
    15301 gattccacta ctatgaaggt cattccatgc aaacagtgac ctatccagta cgtgtgatgg
    15361 ccggactagg ggcccattca gtcattgtga ctaatgcatg tggtggtgtc aacgaatcat
    15421 ttgtaccagg agatttgatg ctgattaccg atcatatcaa ttttactggt gaaaatcctt
    15481 tgatcggtcc gaatgaagat gcaatgggcc ctagatttcc agatatgagt caagcttata
    15541 cgcctgacta tcaagcaaca gcaaaagcag cagcagaaaa actaggtttg gatcttaaag
    15601 aaggcgtata tatgggctat tctggaccaa cttatgaaac gcctgcggag atccgaatgt
    15661 ctcggacaat aggagccgat gcagtaggga tgtcgactgt tccagaagtc atcgtagcag
    15721 ctcacagcgg attaaaagtg ttaggaatct cttgtattac caacttagca gcaggtatgc
    15781 aagcgaatct gagccatgaa gaagttgtag aaacaactca gagagtcaaa caatctttta
    15841 aagcattggt taaagaaaca ttgacattat tgtaatagga ggaacaataa aatgagtgtg
    15901 catattgaag cgaaagaagg cgagatcgcc gataagattt tacttccggg agatcctttg
    15961 cgagctaaat atattgcgga gacttttttg gaagatccag tttgttacaa tcaggtacgc
    16021 ggtatgttag gctttaccgg caaatataaa ggagaacgta tttctgttca aggaacagga
    16081 atgggaatgc catcagccac catctatgcc catgaattga tccagtcgta tggtgtaaag
    16141 aaattgattc gagtaggtac atgcggtgca ttgtcaaaag acgtccatgt tcgtgattta
    16201 gtactagcac aaggtgcagc gaccagttct tcgatgatcg aaaaaaattt ccaagccttt
    16261 cattttcctc caatcagtga tttcaatcta ttactgaaag catatgagat agctaaagaa
    16321 aagggctata cggttcatgt aggaaatgtt ctttcggaag attctttcta caaagatgat
    16381 ttgacagaga cgttccaatt agctgaactt ggcgttttag gagtggaaat ggaagcagcg
    16441 gctctttatt acttaggcgc gaaataccat gtgcagacat taagtttgat gaccgttagc
    16501 gaccatttga tcaccggtga agaaacaaca gctgcagaac gtcaatctac gttcaatgaa
    16561 atgatcgaag taggattgga aacagcgata gcagaataag aaggaaatga aaaagctgcc
    16621 ctgcattaag taataagaac atccaagcaa aaatagctcc tcatgttttt gacttggctg
    16681 gacttattat tgcaaatgta agcttttgaa caccgtttac caagaggttg tgaatctgcc
    16741 gtttaactct gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat
    16801 tgtgacttgt gaccgagaag ttggcaggag aaaaccgttt atacgaaaaa agggactgtg
    16861 acaaatcgtg tcacagtccc ttttttatag tgttttttat aggtagtatt ttttagcaac
    16921 tgttaagtca tcatttaatt cataaacaag tggttgacct gttggaattt caagatccat
    16981 gatgtcttcg tcagagatac cttcgatatg tttagctagg gcgcgtaatg agttaccatg
    17041 tgcagctact aaaacagttt tgttgtctag taatgctgga gcaatttcat cttgccagaa
    17101 tggcaaagcg cgttctaatg tcactttcaa gttttctcca ccaggaatat cgcgtttgtc
    17161 taacattgcg taacgacggt catttgctgc agaaccttcg tcagtttctt ccattaatgg
    17221 aggaagagta tcgtaagaac gacgccagat gtgcacttgt tcgtcgccgt atttttcagc
    17281 tgtttctttt ttgttcaaac cttgtaattt accatagtga cgttcgttta agcgccatga
    17341 tttgatttgt ggtacccaaa gttgatctga atattctaag atcaagttac atgttttgat
    17401 tgcacgagtt aaaacagaag tgtaagcaac atcaaattcg attccagctt ctttgatttt
    17461 gcgtccgcct tcttttgctt cttcgattcc ttctggtgct agatttacat cagcccaacc
    17521 agtaaattgg ttcaatgcat tccattcact aagaccgtga cgagaaaaaa ctaattttgg
    17581 catgtttagc tctcctttta agataggttt atatttctga aattttcagt tctgttttat
    17641 tgtacaattt tttggaagag atttccatcg aaaaattaga taagattcta tgagaaaaca
    17701 agtagagaat ataaaaaaac gaacagtgcc cacgcaactg ttcgttttta ggtattagat
    17761 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
    17821 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
    17881 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
    17941 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
    18001 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
    18061 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
    18121 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
    18181 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
    18241 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
    18301 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
    18361 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
    18421 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
    18481 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
    18541 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
    18601 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
    18661 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
    18721 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
    18781 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
    18841 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
    18901 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
    18961 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
    19021 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
    19081 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
    19141 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
    19201 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
    19261 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
    19321 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
    19381 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
    19441 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
    19501 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
    19561 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
    19621 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
    19681 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
    19741 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
    19801 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
    19861 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
    19921 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
    19981 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
    20041 gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata
    20101 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
    20161 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
    20221 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
    20281 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
    20341 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
    20401 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
    20461 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
    20521 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
    20581 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
    20641 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
    20701 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
    20761 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
    20821 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
    20881 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
    20941 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
    21001 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
    21061 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
    21121 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
    21181 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
    21241 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
    21301 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
    21361 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
    21421 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
    21481 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
    21541 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
    21601 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
    21661 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
    21721 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
    21781 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
    21841 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
    21901 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
    21961 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
    22021 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
    22081 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
    22141 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
    22201 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
    22261 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
    22321 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
    22381 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
    22441 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
    22501 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
    22561 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
    22621 ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
    22681 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
    22741 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
    22801 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
    22861 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
    22921 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
    22981 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
    23041 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
    23101 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
    23161 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
    23221 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
    23281 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
    23341 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
    23401 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
    23461 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
    23521 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
    23581 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
    23641 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
    23701 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
    23761 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
    23821 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
    23881 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
    23941 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
    24001 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
    24061 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
    24121 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
    24181 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
    24241 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
    24301 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
    24361 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
    24421 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
    24481 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
    24541 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
    24601 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
    24661 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
    24721 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
    24781 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
    24841 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
    24901 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
    24961 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
    25021 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
    25081 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
    25141 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
    25201 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
    25261 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
    25321 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
    25381 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
    25441 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
    25501 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
    25561 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
    25621 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
    25681 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
    25741 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
    25801 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
    25861 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
    25921 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
    25981 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
    26041 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
    26101 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
    26161 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
    26221 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
    26281 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
    26341 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
    26401 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
    26461 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
    26521 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
    26581 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
    26641 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
    26701 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
    26761 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
    26821 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
    26881 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
    26941 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
    27001 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
    27061 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
    27121 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
    27181 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
    27241 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
    27301 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
    27361 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
    27421 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
    27481 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
    27541 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
    27601 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
    27661 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
    27721 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    27781 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    27841 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    27901 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    27961 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    28021 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    28081 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    28141 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    28201 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    28261 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    28321 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    28381 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    28441 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    28501 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    28561 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    28621 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    28681 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    28741 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    28801 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    28861 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    28921 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    28981 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    29041 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    29101 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    29161 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    29221 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    29281 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    29341 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    29401 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    29461 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    29521 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    29581 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    29641 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    29701 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    29761 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    29821 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    29881 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    29941 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    30001 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    30061 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    30121 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    30181 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    30241 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    30301 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    30361 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    30421 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    30481 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    30541 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    30601 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    30661 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    30721 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    30781 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    30841 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    30901 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    30961 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    31021 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    31081 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    31141 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    31201 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    31261 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    31321 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    31381 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    31441 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    31501 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    31561 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    31621 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    31681 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    31741 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    31801 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    31861 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    31921 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    31981 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    32041 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    32101 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    32161 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    32221 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    32281 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    32341 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    32401 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    32461 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    32521 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    32581 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    32641 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    32701 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    32761 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    32821 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    32881 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    32941 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    33001 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    33061 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    33121 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    33181 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    33241 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    33301 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    33361 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    33421 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    33481 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    33541 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    33601 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    33661 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    33721 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    33781 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    33841 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    33901 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    33961 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    34021 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    34081 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    34141 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    34201 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    34261 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    34321 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    34381 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    34441 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    34501 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    34561 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    34621 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    34681 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    34741 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    34801 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    34861 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    34921 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    34981 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    35041 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    35101 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    35161 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    35221 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    35281 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    35341 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    35401 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    35461 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    35521 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    35581 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    35641 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    35701 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    35761 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    35821 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    35881 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    35941 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    36001 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    36061 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    36121 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    36181 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    36241 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    36301 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    36361 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    36421 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    36481 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    36541 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    36601 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    36661 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    36721 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    36781 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    36841 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    36901 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    36961 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    37021 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    37081 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    37141 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    37201 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    37261 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    37321 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    37381 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    37441 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    37501 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    37561 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    37621 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    37681 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    37741 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    37801 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    37861 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    37921 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    37981 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    38041 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    38101 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    38161 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    38221 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    38281 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    38341 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    38401 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    38461 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    38521 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    38581 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    38641 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    38701 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    38761 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    38821 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    38881 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    38941 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    39001 tggaatgatt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    39061 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    39121 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    39181 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    39241 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    39301 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    39361 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    39421 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    39481 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    39541 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    39601 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    39661 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    39721 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    39781 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    39841 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    39901 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    39961 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    40021 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    40081 ccaacggcag gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt
    40141 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    40201 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    40261 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    40321 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    40381 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    40441 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    40501 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    40561 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    40621 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    40681 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    40741 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    40801 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    40861 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    40921 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    40981 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    41041 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    41101 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    41161 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg
    41221 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    41281 gcaagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    41341 tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac
    41401 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg
    41461 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    41521 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    41581 cagatgccgg agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc
    41641 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    41701 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    41761 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    41821 ttccttaccc acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    41881 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct
    41941 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    42001 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    42061 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    42121 gacctacagc acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta
    42181 tgatagaagt ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat
    42241 tttgcaaaat ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag
    42301 gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca
    42361 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    42421 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac
    42481 agtctgaaca ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg
    42541 gttgttcttc tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc
    42601 agacataaag aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc
    42661 cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga
    42721 atgcgtcttt gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt
    42781 tgtcataagg aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag
    42841 cttttctgcc agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt
    42901 gataggctac aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc
    42961 cataaaaact tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt
    43021 attgaccaga aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac
    43081 tagaactttc taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg
    43141 caggcacatg gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag
    43201 tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat
    43261 agaatgtaaa atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg
    43321 tcaaattatc tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta
    43381 gtggcaatgc attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga
    43441 agccatattt actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc
    43501 catccatcag caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga
    43561 aacctaattt atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga
    43621 taccactgta accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc
    43681 ggtaaaacga gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag
    43741 gatcagtata taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac
    43801 tgttcgtatt gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat
    43861 tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa
    43921 ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc
    43981 cccatgccac cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat
    44041 cgtcttgttc tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca
    44101 gataggcata acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca
    44161 atggaacaag ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat
    44221 ttttcaacgg tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac
    44281 ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt
    44341 tcatgattag atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg
    44401 cctcgccatt tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc
    44461 ctgccagtaa agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg
    44521 tcaatagacg ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa
    44581 aactggataa aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca
    44641 taacacgatg aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga
    44701 tgatctccga atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat
    44761 gcccccagcg aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc
    44821 cgaccttaag caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga
    44881 acaccagtgg cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc
    44941 ctagagaagc attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc
    45001 tggcatgaaa attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc
    45061 caggatagat tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac
    45121 tagctgctgc gtagcgtccg ttttctctaa agaatgactt taaatttttt ttcatattta
    45181 ttttccacca aatt
//
LOCUS       DAYYJC010000012        44939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000012 DAYYJC010000000
VERSION     DAYYJC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.012"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..1189)
                     /locus_tag="ACHA9N_000651"
     CDS             complement(38..1189)
                     /locus_tag="ACHA9N_000651"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9084137.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ACHA9N_000652"
     CDS             complement(1202..1639)
                     /locus_tag="ACHA9N_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9084138.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ACHA9N_000653"
     CDS             complement(1654..3420)
                     /locus_tag="ACHA9N_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9084139.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ACHA9N_000654"
     CDS             3684..4361
                     /locus_tag="ACHA9N_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9084140.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ACHA9N_000655"
     CDS             4364..5491
                     /locus_tag="ACHA9N_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084141.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ACHA9N_000656"
     CDS             complement(5691..6131)
                     /locus_tag="ACHA9N_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9084142.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ACHA9N_000657"
     CDS             complement(6142..8175)
                     /locus_tag="ACHA9N_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9084143.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ACHA9N_000658"
     CDS             complement(8177..9880)
                     /locus_tag="ACHA9N_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9084144.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA9N_000659"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ACHA9N_000659"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9084145.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ACHA9N_000660"
     CDS             complement(10963..11988)
                     /locus_tag="ACHA9N_000660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9084146.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ACHA9N_000661"
     CDS             complement(12197..13066)
                     /locus_tag="ACHA9N_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9084147.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ACHA9N_000662"
     CDS             complement(13525..15417)
                     /locus_tag="ACHA9N_000662"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084148.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ACHA9N_000663"
     CDS             complement(15414..17141)
                     /locus_tag="ACHA9N_000663"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084149.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ACHA9N_000664"
     CDS             complement(17157..17807)
                     /locus_tag="ACHA9N_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9084150.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ACHA9N_000665"
     CDS             18011..18307
                     /locus_tag="ACHA9N_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9084151.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ACHA9N_000666"
     CDS             18479..19099
                     /locus_tag="ACHA9N_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9084152.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ACHA9N_000667"
     CDS             complement(19191..20357)
                     /locus_tag="ACHA9N_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9084153.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ACHA9N_000668"
     CDS             complement(20369..20722)
                     /locus_tag="ACHA9N_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9084154.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ACHA9N_000669"
     CDS             20850..21872
                     /locus_tag="ACHA9N_000669"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9084155.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ACHA9N_000670"
     CDS             21891..22052
                     /locus_tag="ACHA9N_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084156.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ACHA9N_000671"
     CDS             complement(22311..22745)
                     /locus_tag="ACHA9N_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084157.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ACHA9N_000672"
     CDS             complement(22855..24714)
                     /locus_tag="ACHA9N_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9084158.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            24715..24855
                     /locus_tag="ACHA9N_000673"
     CDS             24715..24855
                     /locus_tag="ACHA9N_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9084159.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(24832..25992)
                     /locus_tag="ACHA9N_000674"
     CDS             complement(24832..25992)
                     /locus_tag="ACHA9N_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9084160.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA9N_000675"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ACHA9N_000675"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9084161.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ACHA9N_000676"
     CDS             complement(27739..28437)
                     /locus_tag="ACHA9N_000676"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9084162.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ACHA9N_000677"
     CDS             complement(28394..30055)
                     /locus_tag="ACHA9N_000677"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9084163.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ACHA9N_000678"
     CDS             30273..31673
                     /locus_tag="ACHA9N_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9084164.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ACHA9N_000679"
     CDS             complement(31925..33004)
                     /locus_tag="ACHA9N_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084165.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ACHA9N_000680"
     CDS             complement(33090..34049)
                     /locus_tag="ACHA9N_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9084166.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ACHA9N_000681"
     CDS             complement(34061..35572)
                     /locus_tag="ACHA9N_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9084167.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ACHA9N_000682"
     CDS             complement(35594..36157)
                     /locus_tag="ACHA9N_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9084168.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ACHA9N_000683"
     CDS             complement(36163..37026)
                     /locus_tag="ACHA9N_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084169.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ACHA9N_000684"
     CDS             complement(37040..37936)
                     /locus_tag="ACHA9N_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084170.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ACHA9N_000685"
     CDS             complement(38111..39400)
                     /locus_tag="ACHA9N_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084171.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ACHA9N_000686"
     CDS             complement(39372..40889)
                     /locus_tag="ACHA9N_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9084172.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ACHA9N_000687"
     CDS             41087..41977
                     /locus_tag="ACHA9N_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9084173.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA9N_000688"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ACHA9N_000688"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9084174.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ACHA9N_000689"
     CDS             complement(42886..44358)
                     /locus_tag="ACHA9N_000689"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9084175.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DAYYJC010000013        43448 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000013 DAYYJC010000000
VERSION     DAYYJC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.013"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..453)
                     /locus_tag="ACHA9N_000690"
     CDS             complement(<1..453)
                     /locus_tag="ACHA9N_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084176.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKIS"
     gene            complement(464..1132)
                     /locus_tag="ACHA9N_000691"
     CDS             complement(464..1132)
                     /locus_tag="ACHA9N_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9084177.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1262..1846)
                     /locus_tag="ACHA9N_000692"
     CDS             complement(1262..1846)
                     /locus_tag="ACHA9N_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9084178.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2177..2410)
                     /locus_tag="ACHA9N_000693"
     CDS             complement(2177..2410)
                     /locus_tag="ACHA9N_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9084179.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2425..3375)
                     /locus_tag="ACHA9N_000694"
     CDS             complement(2425..3375)
                     /locus_tag="ACHA9N_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9084180.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3372..4214)
                     /locus_tag="ACHA9N_000695"
     CDS             complement(3372..4214)
                     /locus_tag="ACHA9N_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9084181.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4216..4935)
                     /locus_tag="ACHA9N_000696"
     CDS             complement(4216..4935)
                     /locus_tag="ACHA9N_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9084182.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5483..5785
                     /locus_tag="ACHA9N_000697"
     CDS             5483..5785
                     /locus_tag="ACHA9N_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084183.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            5857..7083
                     /locus_tag="ACHA9N_000698"
     CDS             5857..7083
                     /locus_tag="ACHA9N_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084184.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7171..7563)
                     /gene="rpsI"
                     /locus_tag="ACHA9N_000699"
     CDS             complement(7171..7563)
                     /gene="rpsI"
                     /locus_tag="ACHA9N_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9084185.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7579..8007)
                     /gene="rplM"
                     /locus_tag="ACHA9N_000700"
     CDS             complement(7579..8007)
                     /gene="rplM"
                     /locus_tag="ACHA9N_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9084186.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8541..9008)
                     /locus_tag="ACHA9N_000701"
     CDS             complement(8541..9008)
                     /locus_tag="ACHA9N_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9084187.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9128..10666)
                     /locus_tag="ACHA9N_000702"
     CDS             complement(9128..10666)
                     /locus_tag="ACHA9N_000702"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9084188.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10746..11432
                     /locus_tag="ACHA9N_000703"
     CDS             10746..11432
                     /locus_tag="ACHA9N_000703"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9084189.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11633..12160)
                     /gene="msrA"
                     /locus_tag="ACHA9N_000704"
     CDS             complement(11633..12160)
                     /gene="msrA"
                     /locus_tag="ACHA9N_000704"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9084190.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12244..12921)
                     /gene="pgmB"
                     /locus_tag="ACHA9N_000705"
     CDS             complement(12244..12921)
                     /gene="pgmB"
                     /locus_tag="ACHA9N_000705"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9084191.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12918..14048)
                     /locus_tag="ACHA9N_000706"
     CDS             complement(12918..14048)
                     /locus_tag="ACHA9N_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9084192.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14048..16231)
                     /locus_tag="ACHA9N_000707"
     CDS             complement(14048..16231)
                     /locus_tag="ACHA9N_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9084193.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16228..17253)
                     /locus_tag="ACHA9N_000708"
     CDS             complement(16228..17253)
                     /locus_tag="ACHA9N_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9084194.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17298..18125)
                     /locus_tag="ACHA9N_000709"
     CDS             complement(17298..18125)
                     /locus_tag="ACHA9N_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9084195.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18122..19189)
                     /locus_tag="ACHA9N_000710"
     CDS             complement(18122..19189)
                     /locus_tag="ACHA9N_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9084196.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19215..20051)
                     /locus_tag="ACHA9N_000711"
     CDS             complement(19215..20051)
                     /locus_tag="ACHA9N_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084197.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20048..20917)
                     /locus_tag="ACHA9N_000712"
     CDS             complement(20048..20917)
                     /locus_tag="ACHA9N_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084198.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20987..22270)
                     /locus_tag="ACHA9N_000713"
     CDS             complement(20987..22270)
                     /locus_tag="ACHA9N_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084199.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22284..23969)
                     /locus_tag="ACHA9N_000714"
     CDS             complement(22284..23969)
                     /locus_tag="ACHA9N_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9084200.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24244..25269
                     /locus_tag="ACHA9N_000715"
     CDS             24244..25269
                     /locus_tag="ACHA9N_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084201.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(25864..29517)
                     /gene="rpoC"
                     /locus_tag="ACHA9N_000716"
     CDS             complement(25864..29517)
                     /gene="rpoC"
                     /locus_tag="ACHA9N_000716"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9084202.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(29596..33213)
                     /gene="rpoB"
                     /locus_tag="ACHA9N_000717"
     CDS             complement(29596..33213)
                     /gene="rpoB"
                     /locus_tag="ACHA9N_000717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9084203.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            33941..34909
                     /locus_tag="ACHA9N_000718"
     CDS             33941..34909
                     /locus_tag="ACHA9N_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084204.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            34927..35832
                     /locus_tag="ACHA9N_000719"
     CDS             34927..35832
                     /locus_tag="ACHA9N_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084205.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            35829..36635
                     /locus_tag="ACHA9N_000720"
     CDS             35829..36635
                     /locus_tag="ACHA9N_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084206.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            36792..37760
                     /locus_tag="ACHA9N_000721"
     CDS             36792..37760
                     /locus_tag="ACHA9N_000721"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9084207.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38077..38601)
                     /locus_tag="ACHA9N_000722"
     CDS             complement(38077..38601)
                     /locus_tag="ACHA9N_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9084208.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            38888..40699
                     /gene="scm"
                     /locus_tag="ACHA9N_000723"
     CDS             38888..40699
                     /gene="scm"
                     /locus_tag="ACHA9N_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9084209.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(40854..41840)
                     /locus_tag="ACHA9N_000724"
     CDS             complement(40854..41840)
                     /locus_tag="ACHA9N_000724"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9084210.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(41863..42429)
                     /locus_tag="ACHA9N_000725"
     CDS             complement(41863..42429)
                     /locus_tag="ACHA9N_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9084211.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(42443..42859)
                     /locus_tag="ACHA9N_000726"
     CDS             complement(42443..42859)
                     /locus_tag="ACHA9N_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9084212.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
BASE COUNT        12796 a         9040 c         7796 g        13816 t
ORIGIN      
        1 agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga
       61 aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc
      121 aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct
      181 aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag
      241 cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag
      301 tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt
      361 aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt
      421 atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta
      481 agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat
      541 agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt
      601 cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca
      661 gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta
      721 tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt
      781 tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag acactaacca
      841 acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa
      901 aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa
      961 ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa
     1021 ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga
     1081 ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac
     1141 ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat
     1201 aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta
     1261 attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata
     1321 gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg
     1381 agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga
     1441 tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt
     1501 caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt
     1561 tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt
     1621 tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat
     1681 taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa
     1741 aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag
     1801 tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag
     1861 ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat
     1921 tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag
     1981 agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa
     2041 tagactcatc taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta
     2101 tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc
     2161 ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt
     2221 tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct
     2281 cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct
     2341 tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa
     2401 tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt
     2461 ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt
     2521 aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc
     2581 atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa
     2641 gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga
     2701 aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt
     2761 tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt
     2821 ttcaatatac ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc
     2881 taactgtttt tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg
     2941 aggatcttcc ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact
     3001 tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca
     3061 agcatttcct ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt
     3121 tttttgaaca tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat
     3181 actgtgtaaa tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt
     3241 agtaactact acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat
     3301 tgaacatgcc ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt
     3361 ccattttctc atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt
     3421 agaaaactaa ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg
     3481 ttaaaactat aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa
     3541 ataaatatca tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt
     3601 aacattgcaa caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt
     3661 aatagaacca tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt
     3721 aatggatcaa aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact
     3781 aaaaccgaaa cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca
     3841 aataaaatat gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt
     3901 gctaaaaaag aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt
     3961 tttataaaag taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa
     4021 ttaataccta aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca
     4081 cccataagag acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct
     4141 ataactacag atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata
     4201 aaattttgaa gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt
     4261 taaattttct tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa
     4321 aagaataata ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac
     4381 tgtttttccc tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa
     4441 gtctatccct acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct
     4501 tgctaaaagt acacgttgaa attgtcctcc agataattct cctatttgat gattagaata
     4561 ttttcccata tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat
     4621 tctttgaaaa actttcattc cggcatatgt tcccatagat acacattctt ttacttttat
     4681 tggaaaagta aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt
     4741 attgatatct tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc
     4801 tttaagcaat gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc
     4861 ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt
     4921 tttcacttct aacataactt tctccttcta aatgataatg attatcacga tcatatggta
     4981 tacaaaaatt atattttagt caaccctaaa ttttatagtt tagatttttt tctgttgctg
     5041 aagattagaa tgacaaaact atttgaaact acgcgtatag taagtagctt tcaaaatata
     5101 taaaaaagga gcgttctcaa atgagaacat gctcctaaaa ctactttatt ggcgtatgga
     5161 aaaattatac ttgaagtgga tagcgggttg aataagttga taagcatcaa aacatttata
     5221 aaaattattc aagactatgt ctgttccata tacaaatatg aaaatcagta aactccattt
     5281 aacttttttc gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa
     5341 aaaactattt ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc
     5401 gagctagaaa ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt
     5461 tgttctggga aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct
     5521 cgccgtaaac tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt
     5581 cataactact attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca
     5641 aacctgtgca tttctaggaa tcgataataa tactctagat aagtggattc gtatatataa
     5701 tttcccagta ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt
     5761 tatgaataca tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga
     5821 aatatactgc cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg
     5881 aaatataaaa ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca
     5941 gaaaagtatc aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg
     6001 aagaagaaaa tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga
     6061 aaaataacgt ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa
     6121 acaattagat caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt
     6181 cggttgcata ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact
     6241 gagataatat cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata
     6301 aaaggatatg ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat
     6361 cgaactacta aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa
     6421 agaatacaag aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca
     6481 gtaaatgatg attttaataa tcatttactt acttttcatg attacacact ttttatgatt
     6541 accctgtatc ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat
     6601 tttgaaaaac aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt
     6661 acaaaaggtc gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt
     6721 gaatggaaat cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat
     6781 cagtatctct ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat
     6841 tacctaaact atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct
     6901 ccacacaaac tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat
     6961 caaatttcaa atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca
     7021 ccagatatag ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat
     7081 tgatatgtgt tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa
     7141 agtgccagaa acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg
     7201 tgctttctta agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc
     7261 agcacgttta agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc
     7321 atgacggata gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc
     7381 atatgcgcct tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc
     7441 atgtggaatg tattcttcaa catctttttt atttacagta attttgccag ttcctggtac
     7501 tagacgtacg cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc
     7561 caatgaaatt ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc
     7621 tgcatgtgga tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa
     7681 agtgttttta ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc
     7741 acgcaattcg ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata
     7801 aattttgtca gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac
     7861 gaaatcgcca gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga
     7921 tgcaacaact gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt
     7981 acgttctact tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt
     8041 aagttctgat gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac
     8101 catttaacat attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa
     8161 tttatatgaa actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa
     8221 tgaaaaacaa aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg
     8281 gtgttctcct gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt
     8341 tttttaaaat tgtttcttgt gactcggagc tgaacggcag gcttacaacc tcttggtaaa
     8401 cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta
     8461 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctctttttcg
     8521 gtcaagttca acaacagttt ttacgcgtcg atctctggcg ctttgcccaa atgagcttgg
     8581 atcatccaga tacgtttttc tgtggctgtt ttgaattcga taaagatatc ttgtgttgga
     8641 tcgtcgcctt cttctccaga aacttcgatt cctttttggt aaagatctgc tagataacga
     8701 tagcctgcaa caagtgtttc caaacgttct ggaatagtac gatcccagcg gccaatttca
     8761 tcagggattt ttgtgttatc tgcaaattca cgcaatgtag aataaggtga accgtctaat
     8821 gtgatcaagc gttcggatac ttcatctaac tgagcgttca aatcatccat gaactcatcc
     8881 atcattgggt gcaatgtaag gaaacctggt cctctcatgt accaatgtgt ttggtgaaca
     8941 acaacagaca tttggcttag atctgcaact aattgattta atacttcttt ggtttgttgg
     9001 aatttcatct ttcttgcctc ctaacatttt cttacactta aaataatagt agactttcaa
     9061 tgaaaagtac aacaaaatca tttacctgag aacgattttc agttcgcttt tttatccgta
     9121 aagccaatca tttttcagaa tcagctaatt cttctcgcag ttgagcaaat tgctgataag
     9181 tctgtgctaa atgcttttgt gtcgaaacaa ataatggata gattttctga tattttttat
     9241 gatcagtttc ttgtactcca acttgctctt tcaattctat caattcctgt gcttcactga
     9301 tactttggat ctttccgatg cttctccagc ctaaaatcac agctcctaat gcggatgctt
     9361 ctgtgacagt agggaaaaca agcgggtaac ccagtacatc agccatcatc tgacgaaaaa
     9421 tcggagattg agcaaacccg cctgtagcac ggatctctgt gagctgattt tcctggcaaa
     9481 ctccttttaa aatccgataa aggttgagac agatcccttc tagaatcgcc ttcatcattt
     9541 ctttttctgt atgttgccgc tgtaaaccga taaaactgcc aaacgcatct gcgtcccaaa
     9601 gcggtgctcg ctcaccaagt aaatagggat gaaaaatcag accattggca cccgctggca
     9661 caaaagcgat agccgaaagc agtgcatcat atggattttc tcctttttct ttagcagatt
     9721 gaacgacttc ctgtaaaaat gtatgccctg cccaatcaaa gatgccagca ccattgctag
     9781 tcgctcctcc cactacccaa tgtttctgat ccagaacata acaaaacgtc tccatttctg
     9841 gatggaactg aggctgagta gtaataaaac gaagtgctcc gctcgttcct acggtcaaag
     9901 cggcgatcgt atcaccaaaa gcacctaacc ctagattaga aagcggcccg tctgcccctc
     9961 ccaaaacaaa tgatatatca tttggcaaac ctagttctgc tgctgttttt gatggtagac
    10021 ctgttacttg ataggtggaa ggaaagactt gcggcaattg ctctattcga atgttcaagt
    10081 agtcaagcac ttcttctgcc cattggaatt catggatatc aaaatatcca gttcctgaag
    10141 ctgttccgta gtcgctgatc agttcgccaa acaatctata aaagatatag tctttgatac
    10201 cgacaaaata agcagttttt ttgaataatt caggttggct gttttgcagc cacttgattt
    10261 tgttcaatgg tgtcattgga tgaatcggcg tcccagtctg ccgataaaat tctttgccaa
    10321 gcacggtata tttcagtttt tctgcttcgt ccgctgctcg gttatctgcc caagtgatca
    10381 ttcgagataa cggtgttttc tcttcatcca acaatatcag gccatgcatc gcagaggaga
    10441 aggagacaaa ttgaatgatt gtgtctgaag gcaactgttt aattacttct tttataacgg
    10501 tgcagacacc ttgaaagatc aattcctgat cttgttctgc catcccttgt gtttcttgga
    10561 tcaaagggta gcgaaaataa gcggatgctg cttcttgggc ttcatctgtg aaagcaactg
    10621 ctttggtttg tgtggttccg atatctaatc cgattgaaac gttcattttt tcacctcaaa
    10681 ttttatgtat gtacaaaaat ttagtttaag cttgcgtatc ttggttttga aaagggggag
    10741 atggcgtgtt actttatttt atcattggtt gctgtatcgg atcattttta tgtttaaccg
    10801 cccagcggat tccgttaggt cactctatta tttatcctag atctcattgt gtcaaatgct
    10861 gtcgttcgct ttcttggtat gaattgattc cgattctctc gatcatcgtc caacgatttc
    10921 gctgccgcta ttgcaggtgt cggttgccct tctactattt gttggcagaa gccttatgtg
    10981 gtggtctttt tgcctggttc tttacttttt cttccgcaag aaatttttcg acgttcatat
    11041 ggatgctttc ggctttgcta ttttcgttga tggatttatt ttacttcatg gtccattctc
    11101 acacgctatt ctgttcttgg gcaattttat ggattttttg gttgcaggcc ggcgtcttcc
    11161 aatggcaaag tgtgctgtta acgtttatcg taggcggtgt gtgccttcgc tatggtcaat
    11221 cctatctcgg agctggcgat actctgttga tgctttcttg gagtggtggc ttgtctctag
    11281 aagagttgct tcagatcttg tttcttgcta gtctgtttgg aatatccgtt ttttctgttt
    11341 actttatctg tcataaaaaa aagttggaaa agctgccttt cattcctttt ttgagcagtg
    11401 cgttgctgat tgtccttcat cttaggtatt gaaaaaaggt tgtgacaaac gacttgtcac
    11461 aacctctgat atatagtgtt ctcttaccaa atccttgtga cttggagcta ggcgacaggt
    11521 tcacaacctc tccactaaat ggtgttcaat cagctgaccc tcagtattag ctcaaaatgc
    11581 tggttttaca acctctgata tacggtgttc cataagtagc tttttcggct tcttatttct
    11641 tgacgcccca atgtcgacga aggaaacctg cacgtccaga accttctcga taaagattgt
    11701 aatggagctt gtttttcttg taataatcct gatggtaatc ttctgcagga taaaattcgc
    11761 ttcttggttc gatttttgtc acgattggct tatcgaatcg cccgctgtct tgcaatgctt
    11821 gttttgaacg ctctgcaata tttcgttctt cttcatcatt atagtagatc actggacgat
    11881 aagaatcacc gcgatcagcg aactgtccta atgcgtcagt cggatcggtt tgctgccaat
    11941 agatttccac caactgatca taagaaataa cttctggatc atattcgatc tctactgctt
    12001 ctgtatgccc tgttgtgcct gttgttactt gttcataagt tggatttggt acatgcccgc
    12061 cagtataacc ggatgtcaca gaaatgatcc ctggctgctc ttcaaatggt ttgaccatac
    12121 accaaaagca tccgcctgcg aaaactgctt tttttgtcat ataaaatccc ccttttttct
    12181 catcatacct tgatttccaa aaggaggata acgttttgct tgtgacagaa aaaacggaaa
    12241 ctattatata cgtgatacgg cttctgataa atgccaaaac gaacgtactt gatcaaaaac
    12301 ggttaaatca ggaatcacat attcagcaga aggaagattt atgttttcac ctattcccaa
    12361 tacatgcatg ttaccagcca aagctgcttg acaaccagct tctgaatctt caatgaccaa
    12421 gcatgcattt ggcggtacat tcaattgctg tgctcctttg agaaaaactt ctggatcagg
    12481 ttttgctttt gatacttgcg tgccgtcaat aaggacatca aaataacttg tcaaattcgt
    12541 tttttctaaa atgaggcgtg catttttgct tgctgatccc aatccgatct tgatgtttgt
    12601 ttttttcaag tactcaagaa cagctattgc accaggcaaa acggatgttt catccatttt
    12661 ttgaatcgct tgaagatata gattgttttt ttgctcagca aaggctgatt tctctttttc
    12721 tgagaatttc tgttccattt ttccaaatga cagcaatcgc tctagtgact cggttctgga
    12781 tattcctttc agttgctcgt tttctttctc agtgaatgga atggacagct ggttagctaa
    12841 aaccagccac gcttcgtaat gataacgagc tgtgtctaca agaacgccat ctaaatcgaa
    12901 tagtgctgct ttgattttca tcgattacct ccttgaaaat gaacgacttg aagtaaagat
    12961 gcgatgtttt tagctgtgcg atgaagatta tcagtggctt gctgtaaagc ttcttgtaaa
    13021 ggcacagctg caggaaggat cggaaaaacg gccgaaatgc caacatctgc tgctttttcg
    13081 cttcctttac caacacttcc gcaaatagct atcacaggaa cgccctctgg tactcttcta
    13141 gcaatcccaa ctggcgcttt tccttttaga ctttgtccat ctagacgtcc ttcgcctacg
    13201 attacgaggt ctgcttgctg tacccgttta tcgaactcca gtaaatccaa tacgtagtcg
    13261 atgccagaga ctattttccc tgaagcaaat gctaacagtc ctgcacccat tccaccacca
    13321 gcactactgc ccgatgcctc gattacttga ggggcaaatc tcatataaaa atcatgcatg
    13381 gcttggtcag ccactgctaa ttcattttct ttcaagcctt tttgtggtcc gaaaacaaaa
    13441 gaagcgcctt cttttccgca taaaagatta gtaacatcag taatgacagt gattttggct
    13501 ttaatcagct caataggcac gttcgtttta tcaactgtag caattttacc tagattttct
    13561 cccagtgctt ctactttttg attatcttga tcgtaaaaag cataacctag accttctgcc
    13621 atgccgatgc cgccatcgtt ggtactactt ccgccaacgc cgatgatgat gtgtcgaaca
    13681 ccttgttgca acaattgtcg tatcagttct cctactcctc gcgtggataa cctaagtggc
    13741 tgttgttggt ttagtgggac atctttcaat ccgacgattt ctgccatttc aaaaatcgca
    13801 gtttgaccgt ctgaagcgta ctggatttct ttgtttttgc caaatgcatt agtaacgact
    13861 gctgtttgcc gcttcaacct taaactgtct gctaatgatg aaaccgttcc ttctcctcca
    13921 tcgcctatag gtagcagatc atattcagcc tgaacaaaaa cttcttgaaa acctgattga
    13981 atggcttgtg ctgcctctaa ggctgtcaga ctttctttga aagaatctgg cgcaatcaga
    14041 atacgcatta ttttcccctc cgttccaaga tcagttcttt ttccagctgg tattggtctc
    14101 cattgacaaa aataggtaat gtttctttcg tctctttttt tattactatt tgtttttttg
    14161 tcaaaacaaa tagcaacctg tgatttttcc aaataaaagg aaaagaaatt ttttcccatg
    14221 ctgaaggaag tcttggagaa atagaaagag ttccattcgt tactgtaata cctgaaaaac
    14281 caaatataac tgctagccat agtgctccta atgaagcagc atgaattccg tcatctgatg
    14341 aatgcggatc tggtcctaaa tcaatcaggc acgcttcttg gaataaatga tacgcccatt
    14401 ccatttcttg gatacggcaa gcctctatgg cgtgaatcgc tttactcaac gatgagtcgt
    14461 ggattgtttt gttttcatag taatgcaaat tcttagaaac gattggttga gaaaaatact
    14521 caggtaataa gtataacagc atgacaagat ctgcttgctt gctgacttgg agttcattta
    14581 cttcgctacg ggcataatca agtaaaatag actggctgcc aggacgttct ttgtaagtgg
    14641 tcaaatcaat aactggctta ctgagatacg tgtcatcttg cggaatcagc tgttctttgt
    14701 taggcactgg caaatataac ttatctaaaa aattttgaca ttctttcaaa aaaagcggat
    14761 cgccagcttg atatttaaca gctaaggcca cattataatg cgccagatag ttcgtatatg
    14821 cattattgtc gatatattct gtgtattcat ctggaccaat gactctgtgt atctctaaat
    14881 gatcatcgac ttttacagct ctgctcagcc aaaaagctgc tgtttcatga atgagcgttt
    14941 ttccctttgt ttctaaaaac gattgatcct ctgttgcttg gacatactct tgaacggcat
    15001 aagcaaggtc tgcactgata tgatgttctg cggcagctga agcaacttct tgtcttgttc
    15061 cggttttgat attcaatgca gcaaatttgg gtgtctcttc ctgaccagtg tgtgcgcttt
    15121 cccatgggaa aagtgcacct tgatagtgat aatctcttgc tttttgccgt gcgccttcta
    15181 atcgatcata acggtacagc agcaaattct ttgctacttc aggaaactga tataaaaaga
    15241 aaggcaggat aaaaatttcc gtatcccaga aaacatgccc tttataacct tcgccagtta
    15301 gtcccttcgc ccctacacta acacttggat cattgcgggg agtcataatc tgtaaatggt
    15361 aagcagcgaa gtccatcgct aactgatcga aagaagaatc gccttggatc gttatccgat
    15421 gctgatgcca aaaatctgtc caataagaca tcgactgctc taaaatttct gtataactaa
    15481 cgcttttctg ggcttgggca atacttttct taaacgggtc tgtttgctga agagaggtag
    15541 agagcacgat gatcttctcc agcgtaaagg acgtattttt tctgatcttt tctgaaaagg
    15601 aagcggtgat ccggcgattt tttgcttgga atacacctgc tttattgaat ttagctgcaa
    15661 tcccaatatc aattttactt tctgttgtct ggtaggatgc actgatcaaa tcgtcaccaa
    15721 atactcgcag ttctttttca accaactgct gtgtaccaaa attggtttgc tgtccatcga
    15781 tacccgtttt cacatctact tggcagtctt cggtcaacgg cttgatgacc actttcaggg
    15841 ctgcctgatg gagattattt tgatcagcaa cacgacaagt aatgacttcc actttttttc
    15901 cttgagcagt ttcaatgagc gcatagcgtt taagctctcc catttctaaa tctagtgccc
    15961 gctcgtatgc aagaactttc atcgtctcaa gagaaaaatg ttcaccgtcg atttttattt
    16021 cccatctgat gacatctggg agattcacta actcggctga ttctgctcct aaaggtcggt
    16081 tatatacacc agctgcaaac attcccctta cttgattccg atagtcttcc tcctggcttc
    16141 ctctgatacc aaaaaatcca tttccaatgg tcatctgact cgcatactta ttgatatact
    16201 ctaaatcaaa ctgtttttct tctactttca taaggtaata acttctcctg tttctgccga
    16261 acgatataaa ttttccacca gattctggat gacatatcct tgccagccat ctgcaatcat
    16321 ctgagtctgg tcaccgagcg cccgattcac aaaatgagcc atacttagtt catgtgggtc
    16381 attaactggc gtggtctttt gtaaggaaac cagctttcca tctacatctt cgaatagttc
    16441 gcctggatac agattagcac ctgcctgatc gccacaaaaa gccacattca gtttgtcttc
    16501 tctgatattc aaagcaaacg aagtattcag ttggatgatc ttgccgtttt ctaattccag
    16561 tgtgccaaaa attgcatctt ctactgtgaa tttgcttgga tcccatttcc cgaatttccc
    16621 tgaatttttt ttcgtaccaa ttttttggaa gctgtacgcc cgaacgcttt ttactttagg
    16681 aaatccagta atatacatcg ctgtatcaag catgtgaatg ccataatcga tcaaaggccc
    16741 gcctccttga agttctttgt cgataaacgt tccccagcct ggaacaccag accgacgaag
    16801 cgcattcgct tctacgtaat aaatatcccc gagaacatca agatgattct tgatcattgt
    16861 tgcttcgtca gaaaaacgat gctggaatcc ataagccaaa atctgcccgt ttttatcggc
    16921 tgtctctgcc atctttcttg cttgatcagc tgtcatagct ggtggtttct cgcacatcac
    16981 tgaagcatta gcttcgaggg cagccaatac atgttcgaag tgaaagcgat tgggtgtaca
    17041 gacactcacg atatcgggag aaatctcatt cagcatttgt gtaactgatg tatacgcatg
    17101 agaaaatcct gtacgtcggc aaaagtcttc tgctcgctgg ctgactggat caaccacagc
    17161 ggccaattct aagacatctg atcttttttg ataataagca gcatgtactt tttctgctac
    17221 ttgtccagcg cctatgattg ctactttctt catttatttc tctctccttt gcttatttac
    17281 ttattgttat tctttctcta ttctttctct aaatcattta aatgacgcgc tgtttccaga
    17341 cagtttttca agtattgcaa tgattcctga taaattttca cttcattttc tcctcgcaaa
    17401 cgacattcat ataccatata accttcatac tcattttgaa gtagcgtttg aaaatggcga
    17461 gtaaaatcaa ttgcgccaga tcctggttgg tagcgatgat tatctgctaa atggatatga
    17521 cccacatact ctttttgcgt agtcaatgct tttgaaatat catcttcttc aatattcata
    17581 tgataaaaat cagcgatgat ctgcacatgt ttcaattgat tagagtcaat gtaatgacgc
    17641 gcatccgcta acaaattgat catatggtct tggtatcggt tcaaaggctc caaataaata
    17701 accgtacctg tttcttcggc tactttatct aaataacgaa gtgattcgct gactgcttta
    17761 tagtctcctt ctttgcttcg gggtgaaacc atcggcggta atcgataagt gaacattccc
    17821 caagctgctg ggataacgat tcctttgcct ccaacctcgc ttagtgcacg caaaatctca
    17881 gcaatttgac gtacaccatt aagacgccgt tcttcgataa agtcaccgat ccacccatcg
    17941 tagccgttac aagctgtcac tactgggatc cctgtttctt tgattgcctg tttgactgtg
    18001 tcgatatttt ctattaacaa cttaccgtcg atttcgtatt cttcaaatcc acagtctttg
    18061 ataaatctaa atttttctaa aatgtctctt gggaaaaatg cttgatcttg cgtaccgaat
    18121 ttcattgttc tcctcctcgg acttgaacgc ccattttgat actttcttct gggtgctggt
    18181 ctacataacg catgtaagct tctgcgcttt ctgcaaatgg cacaactgga tcaatgattt
    18241 cttcacaatc caagtaaccg ttcatcaata attcccaaca tgtgtcctcg attcgtttac
    18301 ggttccatct tgggtaatct gggtttggtt cactagatgc tcgagcaaag acgatttttg
    18361 cattattgaa atgtgcttcg cgaccaagat taaacccagc tggaaaaggt ttagcaaagg
    18421 caacataaga aatgatcccg ccatagccga tccctattaa agaagcttgt aatgcggcag
    18481 cactgccact cgtttcaata actgaatcta ctccacgttt atctgtcaat tcctttagac
    18541 gaaaaccgac atctgctttc ataggatcta gcgtgtaatc ggcaccgtgt ttttctgcta
    18601 attctctacg ctgcgctatg ggatcgatcc caacgatggt agtagcacct gcttttttag
    18661 ctaattgaat cgcaatttgc ccgatagcgc ctaaaccgaa gattgctaca gcatcacctg
    18721 ctcgaacatg tgcttcccgg ataccaccta aagcgaattg agcaggatcg taacatactg
    18781 catttttcca ttgatccaac cgatcaagtt tacgtagttt ataattatca acaccgtttg
    18841 taatcagcgt ttcacgaatg ggtccataag tgcagactaa ctctcctttt tgatactctg
    18901 tcaccaaact gcccgtttct tcgatcgtac cgacaatcat atttcctaat tgaaattcgc
    18961 caaataccac accgcgttgg cttccttctt ctcttggcaa gaataggcgc cattcttctg
    19021 aaaattcttc atcgataaat ggacttaatc ctctaaagtc gacaatttct gttcctggtt
    19081 ttggagtagc gaaaattact cggatacgta cttcgttctt ttcgatctca cggtcttcgt
    19141 aatctttgaa tccggctatt cttggttcta atgcaactaa ttttttcatc ttttctttcc
    19201 tctttctttt ctttttatcc ttttacgccg ccttcgacta aatcaccttt aataaatcgt
    19261 tcagataacg catacataat gatgactggt aaagcggtca ccaatgaagc ggccatcatt
    19321 cgtccccaaa tgtagtctgg tgtactgaat aatgtgttca aaccgattgg taaagtaaat
    19381 ttttcggagg tcgacaggaa aatcgaagca aataaataat cattccacga aaccataaag
    19441 caataaacga agacagaaat gattccagaa atagccaatg gtataatgat ccgaataatg
    19501 atctggatcc ggctaagacc atcgatcatt gctgcttctt caatatcagt cggaatcgtg
    19561 tcaaaataac tttttagcat gaatatcgca gtaggaagtg tttgaacgac catcgtgata
    19621 atcagtgctg tattggtatc atacaaacct agaccggaaa tgattttaaa taatggaaca
    19681 acgatcaaaa taccggaaaa catataaacg gtgtaaaagc tagcattgat tttcgagcga
    19741 gctttgaagc gtagttttga caaagcgtat gccccgctga tcccaatcac tactgcagtt
    19801 acggaggcta tcaaggagat cactaaacta tttttgaaat acatgatgaa tggaaaaatc
    19861 tcagcattga aaatatcctg ataatgttca aaagtccatt ctttcggaaa aatagtttgt
    19921 gtcgttgcaa ttgcttcttg tgaatccttg aatgatgtca tcagcatgat caaaaatgga
    19981 aacaaattga cagctaatag caacaaaatc gtcgcgtaaa ataagatttt cctgctcttt
    20041 ttatttttca tttcaagtcc accttctttc gtgagagcaa gacgaccgct gcaataatga
    20101 tgaacaatag aatcgatata gctgctgctt ttccaagatc attgaacgca aaggctgtct
    20161 gatacaaata aacgcctaag atattgactt tattcgtcag tagatagaca tctgtaaaca
    20221 tatagaacat ccagatagat cgtaaaacaa tcacagttgc tagtactggt ttgattgccg
    20281 gcagtgtcac gatcttgaac ttctgccaag catttgcacc gtccatttca gctgcttcat
    20341 aaagcgtttt gtcgactgtt tgcaaaatag ccaaaaatga aataaacgca tacggatagt
    20401 atctccagat agcaaacaaa acgaccataa agaagctagc aactggctgg tcgaaccaca
    20461 gcggtgcttc gttgaaaagc tgcaacctat ctactaacag atcattgacg attccataac
    20521 tattattgaa catatatttc caagcaaaaa tcaaagaaat agatggcgta acataactta
    20581 ataaaatcag tgaacgcacc gttttcctaa atctgaactt gcggttaagc aaaatagcta
    20641 cggctaatcc taaagctgta ctgcctgcta caactaagaa tgtgtaagca actgttaaac
    20701 ctaaagaacg ataaaattca ctatcactta aaatttcaag ataattgctt aatccgataa
    20761 aaacggcgtc tgagttagga ttcagcggca tatcgaaaaa actgatctga atattggaaa
    20821 gcattggaat cagcactaaa acagctaata aaagcatgct tggtgctaaa agtcccatgg
    20881 ctaattttaa gtcagaatgt ttccttggac gattcattga aacctccccc tgataatctg
    20941 ccaatagcta tctacttttt tcgaacacca ttggcagaac ttcttgttat ttcgtatttt
    21001 tttgtgcgtc agctaaagct tgatcgacat cttcgtttcc gacagttact tggttcactg
    21061 cttttccgat cacacctgag cttgtgatat ctcccatttt cgtgaaattc ttttccccga
    21121 ctagtccaaa gacttgtact tcatcaaaag cactagcaat ctcagatggc agctcgccaa
    21181 aagacttgat gacttcattt tctttgtaac catccagttc gactacttgt ttattcactg
    21241 gttgagcacc accaggagac atcaagaccc acttttccat gtttttcggt tgagataaga
    21301 aagctgtgaa tgcttcagca gcctcttttt gtttctcgtc tagaccagaa gaaatcgtca
    21361 atccagatac tgtgccatag acagcttgat ctttttgtgt tgggatagca aaaccgatat
    21421 tttctgagtt ccccgcttca taaacagaag gcaaaatgta agttgaatag attgccattg
    21481 gtgctgttcc attcatgaaa gcatctttga tttctgtcac atcattagag cctggcatcg
    21541 tgtattggga taagttttga taaaacgaca atgcttctct catcttttcc gtatcgatcg
    21601 ttacctcgcc ttcatcatcc aaaacatttg ctccatttga caaagcaaat tgagagaacg
    21661 cttgctcact cattgttcct tcagcagtag gcatagcaat tccatattgt ttattctctt
    21721 tatctgtgaa atgttgagca atttttaaaa tatcatccca attttctggt tctgaaaaac
    21781 cagcttctga taatttttct ttgtcatacc aaataccttg aacccagccg ctaattggtg
    21841 cagcaatata gctttttcca tcttctgaac gaacaaggtt tttggctccg tcataataat
    21901 tatcttctcc agcttcctcg ataacgtttt ggactgccgt ctgatcaatc agttcatctt
    21961 tatccatcac tttagcaaaa tcctgactga cttccattac ttctggtaat ttaccggaac
    22021 gagctaacgt aacgattttt gtattgaacg ctgattcttc taccggcact tgcttgattt
    22081 tgatgtctgg attttctttt tcaaaatcag caaccagttc attgatcaca cttaatcgtt
    22141 cctgttcgac agaagagtgc atgaattgaa tcgttgtctg ttgtgattcg ttagagcttt
    22201 gactttcacc acaagcagta agaagaaatc cagtagctag cagacaggta gtaacaataa
    22261 tttttttcat ttttttccac ctcttatttt attattttca gccacatata ttcatacggc
    22321 tggaatatca actttcgttc ttgactgcaa tggataattt gtccagaaaa caggtctagg
    22381 taatctcctt ttggtaaaag ataacctgct gattgattgg aaaaattatg gataactaag
    22441 actttctctt ccttcgcttc gcgaataaaa ctgataaaag aatctgctga gtccagtaag
    22501 taaatcaaac tattcggatg gaaggctttt tctttttttc gaatagatag catctgggtc
    22561 aattcaccat atactgctgt tcttaatgga tctgtctgca actctttctc aatatctgct
    22621 aacaaaaatt tttgccgatt gatttttcga ttctctcctg ttttttctac gcctttgtaa
    22681 tcatttcgac ttcccagcac actttgaata taaattgccg gcacgcctgg taacgttacc
    22741 aaaaatgaat gagcagctaa aaagcgtttg cttctccatt ccgtcgtatc tgttggagaa
    22801 cttaacgcat ctagatacgt agcgtttatc tcatatggac tggtcgttcc atctgggttt
    22861 ttcttgtagt ttactcgggc accctctttc ttcagctgtt ccactaattc aaggatcgtt
    22921 ttttccggca gaatacctcg taccggattc aatccgatac cgtcatggga ggctaaaaaa
    22981 ttaaaaaaag ttgttttttt cgatgcgaaa tcgatggttt ttaaccattc tttcaactta
    23041 cttgtattac ccgaacgaat cgcttgaagg gtcaaaggtg gtagcgaaaa ctgatagacc
    23101 atctgtgctt cattctcgcc attcccaaaa taactgatat tatctttgtg tggcacattt
    23161 gtctcagtga tcaataccgt tcctggagct gcttcatcag cgattgcacg gaataattgg
    23221 atgattttat gcgtttctgg caggtgtata caagaagttc ctagttcttt ccataaaaat
    23281 cccacagcgt ccaagcgaat aaatgaagcg cctttttcta agtaaaacaa aagaatatcg
    23341 atcattttca ataaaacttc tggattttta aaattcaagt ctacttgatc ttcgctaaaa
    23401 gtcgtccata cgtgtttttc tttcccgttt ttgtcaatga acgttgtaag taacgggctg
    23461 gtccttggtc tagtcacgct gcttgtatct gtatccagtg gcaaatcaat aaaaaaatct
    23521 ttgtacgtat cattcaagtt taaatattcc tgaaaccaag tactttcggc agacatatga
    23581 ttgcagacaa agtcgaacat taaccgtgtt tcgcttctca tttcttctat atcttcccat
    23641 gtgcccaaac ggtcgttgat tgcctgataa tttacaacgg agaaaccatc atcagatgta
    23701 tatgggaaaa atggcaagaa atgtacaact ctaaatgtat ctgccaaata cttttgataa
    23761 aatttcttaa atgtacgcaa agttggttgc tgctcttcaa aaaattgatc cccataagta
    23821 atcaagaaaa tatcttcttc gtcccaatgc agtttgcctg cctggccttt cttctgctga
    23881 taaacactga ctttttgttc aatcatagag gcggttttct cagacaaatc tgagtcgcga
    23941 taaatcgctt gtaaaagttt tgtatacatc ggattcccct ttcttttttg ggagcgttcc
    24001 caacgatttg atcatattat ggaaaacgct tactgtcaat agcatttttt taaataattt
    24061 tgatatacac aaaaatacaa ataagaaagc ttatgtatca ataattaata gcttcttaaa
    24121 aaaatatggt ctctcatgat catgcaagct attcctaaat tttcaaaatc atagacaaaa
    24181 aagaatgtct attgtactat ggaattattc taaaacgctc ccaaaaagga ggtgcatata
    24241 aaattgaaac ctactattta tgacattgcg aaaaaagcta atgtctcaaa atcaactgtc
    24301 tccagagtat taaataacca gccaaatatc tctgataaag caaaagcaag tgtacttcaa
    24361 gcaatcaaag aattgaatta tgaaccgagc aaaattgcaa gaggactgtc aggaaatggt
    24421 tttgacgcga ttctagcatt atctcatcgt acttctcata cgacctatgg caatcctttt
    24481 ttttcagatg ttattcaatc catttctgcc gtttctgaac aaaataactt cgacttgatt
    24541 ctacaaacgg caaaaacacc agaagaagag ctagaaaaat gcgcgaaaaa gatccaagaa
    24601 aaaatgatcc gaggaattat tattttaagc tctacgatgg atgaaacact tctttttgaa
    24661 ttggatcaat ataatattcc tattgtggta atcggaaaag tcggaaacga ctacacgaac
    24721 atctactcag tggacacgga taatttccag gatagttacg atttggtgga aacactgatc
    24781 aagaatggac accgtcatat cggctgtctc catgctccga cagaagttta tgtatccaat
    24841 gatcgtctca atggttaccg tcaatgctta tttgatcacc acctagatat ccgtaatgaa
    24901 tggattatag acggcggctt tcgcttagaa gatagcatgg cagcaatgaa tgatttaatg
    24961 aagctacgag aaagaccaac tgcgattttt tctaccgatg cattaaaatc attgagtatt
    25021 tacaaagtgg cagaacaatc tggaaagcag atacctgatg atctttcgat cgcaggattc
    25081 agtgatccct tattgtcctc cctattttcg ccacagttga ctcggataga cattccgaca
    25141 aatcagttag gaatcaaagc cacagaactt ttattccaac taatccacca ccaataccct
    25201 gaagaaaaaa gaatcattct ttcaaccgag attactctca cagattcgat ccaacaacaa
    25261 gaaatgtaga acagaattat aaaagaacag aaacactgag gataagtcag ttactctgtt
    25321 cttttttcaa tcactcatcc ctcgttaccc cctcccggta ttttagcgaa tttttcttat
    25381 tttccatctg ttttagatag ctgtggataa ctataatgtt tgtgttaaac tattttttcc
    25441 acagggaaga aaaaactatt tttaaccacg ttgtgtataa gtttttatag ttcgtttttt
    25501 taaacacatt tacccttatt tcaatccatg ttatttgggg atacttatct ctaaaacata
    25561 aatagaataa cagacatgtc tactggtttt gttttattcg aggaattatt caataatagc
    25621 tagcaaaata ttaaaaataa ccgctctatc ttcccatcaa tttattttgc attcaaaaaa
    25681 tgatctatct ttacttttgt tgattgactt ctcttggaag atgatcgttc cgatacctta
    25741 attggatacg aaccgatgtt gttaagatcc tttcccactt ttcttctagt tagacaaatt
    25801 attttagaca aaccaaaaaa gaggttgtga caataatttg tcacaacctc ttttttaaaa
    25861 tatctatttt gtttgattgt tcattgcgtc ttctgcagcc atttgcgctt caatatctga
    25921 aatactgtag acattttcgc ttgctacacc gacttctttt ggttccatgt cacgataacg
    25981 ttgcatacca gtaccagctg ggatgatctt acctataata acattttctt tcaaaccaag
    26041 aagaggatct ttcttaccgc ggatcgcagc atccgtcaag acacgagttg tttcttggaa
    26101 tgatgcagca gataagaaac tatttgtttc taatgatgct tttgtgatac ctagtaagac
    26161 aggtcgagaa gttgcaggaa cgcctcctga gactagcgta ttgtagtttt gatctttaaa
    26221 gtctgcgatg tctaataatg tacctggtaa gatatctgta tcacctggat ccattacacg
    26281 aactttacgc agcatttgac gaaccattac ttcgatatgc ttgtcgccga tttctacccc
    26341 ttgcatacgg taaacacgtt gtacttcacg aagcaagtag ttttcaacag ataatacatc
    26401 gcgaacttgc aacaattgtt ttggatcgat tgatccttct gtcaatggtg caccacggtg
    26461 gatcatatcg ccttctgcta ctttcatacg tgctgtataa ggaacagtat acgtacgtgt
    26521 atctgttttt cctttgattg ttacttcttt ggtacgtgtt gcaggatctt ctgagatatc
    26581 gatgacatcc cctgttactt ctgtgatcac tgcttgccct ttcggattac gtgcttcaaa
    26641 gatttcttgg acacgaggaa gaccttgagt gatatcgtct ccggcaaccc cacctgtatg
    26701 gaatgtacgc atcgtcaact gagtaccagg ttcaccgatt gattgagcgg cgatcgtacc
    26761 aactgcttct ccgacttcaa cgtcagagcc agttgcaagg ttgcgtccgt aacaatgttt
    26821 gcagacacca tgttttgtat tacatgtgaa gacagaacgg attgtcactg tttcgacacc
    26881 tgcatcaacg atttgttttg cgatgtcttc ggaaatcaat tggtttcctg caatgatcaa
    26941 ttcatttgtt tcaggatgac gtacttcttt acgggtgtaa cgtcccacta aacgatcttc
    27001 taatgattcg atgatttcat ttccctcgcg gatggcttgg atttcaagac ctcggtcagt
    27061 accgcaatct tcttcacgga tgattacatc ttgggccacg tcaaccagac gacgagtcaa
    27121 gtaacctgaa tcggcagtct tcaaggctgt atcggtcatc cctttacgag ccccgtgagt
    27181 tgagataaac atttccaaga cggatagtcc ttcacggaag ttcgaaatga ttggcaattc
    27241 catgattcga ccattaggag cggccatcaa accacgcatt ccggcaagct gtgtaaagtt
    27301 ggaaatgtta ccacgagctc cagaatcaga catcatgaag attggatttt tcgcttccaa
    27361 accttccatc agtttttgtt ggatgctgtc ttttgcagca ttccaaacag caatgacacg
    27421 ttcataacgt tcgtcatctg tgatcagacc acgacggaat tgttttgtaa tagtttcaac
    27481 ttgtttatgg gcattttcaa tgatttcttg tttttcattc agtacactga tgtcagcgat
    27541 ccctactgtg ataccggcat gtgtcgaatg tttgtaccct aagtctttca tacggtcaag
    27601 catcttagaa gtttctgtta ctttgaatcg tttgaagact tctgcgatga tgtttcctaa
    27661 gtttttcttc ttgaacggta atactaattc ttgctctttg atatgtgcag gaatatcagt
    27721 accagcttca acaaagtatt tgtccggtgt ttggacggtc aagttgaagt ctgttggttc
    27781 attcagataa gggaattcag gaggcatgat ctcgttgaag atgatcttac caactgttgt
    27841 gatcatcgta cgttcttttt gccattcagt aaatggtttt tcacctaatg agttagggtt
    27901 cacaccgata cgtgaatgta aatgaacata tccgttgcgc catgcgataa cggcttcgtt
    27961 ttgatctctg aagaccattc cttcgccttc gcgtccttct tcttccattg tcaagtagta
    28021 gttacctaga accatatctt gagaaggagt aacaactggt ttaccatctt tagggttcaa
    28081 gatgttttga gcagctaaca tcaacatacg tgcttctgct tgtgcttctt cattcaatgg
    28141 aacgtggaca gccatttggt ctccatcgaa atcggcatta taagcttcac atacaagtgg
    28201 atgcaaacgg attgcgcgtc cttgtactaa aactggttcg aatgcttgga taccaagtcg
    28261 gtgaagcgta ggtgcccggt tcagcaatac tgggtgttcc ttgattacgt cttctaaaac
    28321 atcccatact tcgtcttctt gacgttcgat tttacgtttc gcatttttga tgtttgaagc
    28381 tagttcgcgt tgaacaagct cacgcatcac gaatggtttg aataattcaa tggccatttc
    28441 ttttggaagt ccacattggt acatcttcaa gaaaggtcca acaacgataa cggaacgacc
    28501 tgagtagtcg acacgtttac ctagcaagtt ttgacggaaa cgaccttgtt tccctttcaa
    28561 catatgagaa agagatttca atgggcggtt acctggtcca gtaactggac gtccgcgacg
    28621 accgttatcg atcaatgcat ctactgcttc ttgcagcata cgtttttcat tttgaacgat
    28681 gattccaggt gcattcaagt ctaacaaacg tttcaaacgg ttattacggt tgattacacg
    28741 acggtaaaga tcattcaagt cacttgttgc aaaacgtcca ccttctaatt gaaccattgg
    28801 gcgtaaatct ggtggaataa cagggataac gtccataacc atccagcttg gttgatttcc
    28861 tgaagcgcgg aatgcttcta aaatatccaa acggcggatt gcacgtgtac gtttttgtcc
    28921 ttgtgcagat ttcaattctt ctttcaattc ggctgcttct gcatctagat caacattatc
    28981 aagtaattgt ttaatcgctt ctgcacccat agcagcttgg aattcttgtc catattgttc
    29041 acgtttttcg cgatattcgc gttctgtcaa caattgtttt ttctctaaat tcgtatttcc
    29101 tggttcgatg actacatatg aagcaaaata aatgatttct tccaaagcgc gtgggctcat
    29161 gtctaataca agacccatac gtgaaggaat ccctttaaag taccagatat gtgaaacagg
    29221 agctgctaat tcaatatgtc ccatgcgttc acggcgaact ttcgaacgag taacttccac
    29281 tccgcatcga tcacaaacga ttcctttata acggatacgt ttgtattttc cgcaggcaca
    29341 ttcccagtct tttgtaggac caaaaatccg ctcacagaac aacccttcac gttcaggttt
    29401 taaggtacga tagttaatcg tctcaggttt ttttacttca ccgtaagacc agcttctgat
    29461 tttttcggga gaagccaaac ctatttgcat actttcgaat ttatttacat cgatcaaaag
    29521 gggttccctc catttcattt aatggcaaac cgcaagacgg ctgatcttcg accgccttgc
    29581 agctcataga ccggattagt ctaaattatc ttctgcggtt tcaaagtttt ggatgacatc
    29641 gtctttttca tcttccactt gttcagcagc tttcttttct aattctttgg cagtttgctg
    29701 ttcggcaaat tttgtcaaag catcaacagt gatcaaatcg tcgtcttcat cgtccatgtc
    29761 gcgaagttcg atctctgttt cttcgatatc caagacacgc atgtccagac ctagtgattg
    29821 caattctttc accaatacgc ggaatgattc cggtacacct ggttttggaa ttggttcacc
    29881 cttgacgatt gcttcgtatg ttttcacacg tccgactacg tcatctgatt tgtatgtcaa
    29941 gatttcttgt aaagtataag ccgcaccgta agcttccagt gcccatactt ccatttcacc
    30001 aaaacgttgt ccaccaaatt gagctttacc tcccaatggt tgttgtgtaa ccaatgagta
    30061 aggtccgatt gaacgtgcat gcaatttgtc atcgaccatg tgagcaagtt tgatcatgta
    30121 catcacaccg acagacacac gtccatcaaa tggttcacct gtacgtccgt cgtacagaac
    30181 agttttcgca tcgctggcca taccggcttc acgaactgtt tcccatacgt cttcatcact
    30241 tgcaccatca aatactggcg ttgctacgtg gatacctaac tgacgtgcag ccatacccaa
    30301 gtgtaattcc aatacttgcc cgatgttcat acgtgaaggc acccctaaag gattcagcat
    30361 gatatcgatt ggtgttccat caggtaggaa tggcatatct tcttccggca taatacggga
    30421 tacaacccct ttatttccgt gacgaccggc cattttatcc ccttcatgga ttttacgttt
    30481 ttgaacgata taaacacgaa ctaacatgtt tacgcctggt gacaattcat ctccagcttc
    30541 acgagtaaag attttcacat cgtgaacgat accgccgccg ccatgtggta cacgtaatga
    30601 tgtatcgcgg acttcacgag ctttttcgcc aaagatcgca tgcaatagac gttcttctgc
    30661 agataattct gttacccctt ttggcgtgac tttccctaca agcaaatcgc catcttggac
    30721 ttctgcacca atacggatga tacccatttc gtctaaatct tttaatgcgt cttccccgac
    30781 attagggatt tcgcgtgtga tttcttcagg tcctaatttt gtatcgcggg cttctgattc
    30841 atattcttca atatggatag aagtgtatac gtcatctttt accaaacgac ggctcatgat
    30901 gatcgcatct tcatagttgt acccttccca agtcatgaaa ccaactaaaa cgttttggcc
    30961 tagagccatt tcgccttgtt ccatagatgg tccgtctgcc agagtatccc ctttttcgac
    31021 tttctcaccc aaatgaacga ttggacgttg gttgtagctt gttccggagt ttgaacgacg
    31081 gaattttgtt acatcgtatt tatctaatgc accgttgtcg cggcgtacgc ggatttcaga
    31141 agcatcaacg tattcaacga caccgtcatg tttacataac aaagcagctc ctgagtcatg
    31201 cgctgatttg tattccatac cagtaccaac ccatggtgct tgaggattga tcaacggcac
    31261 tgcttgacgc tgcatgttgg cacccatcaa ggcacggttg gagtcatcgt tttccaagaa
    31321 aggaatacat gctgtcgcaa cggcaactac ttgtttaggt gatacgtcca tgtagtctac
    31381 tttatcaata gatacttcta agttttcact ttgagcacga gccataacaa cgtcttgagc
    31441 aaatgttcca tcttcattca atgggctgtt tgcttgggct acgatgtaat ggtcttcgat
    31501 atcagctgtc aagtagtcga tttggtcagt aacacgacct gtttcacggt ctacacgacg
    31561 ataaggtgtt tcgataaatc cgaatttatt tactttcgca taactggaca aactattgat
    31621 caacccgatg tttgggcctt caggagtttc gatcggacac atacggccat agtgggagta
    31681 gtgaacgtca cgaacttcat aaccggcgcg gtcacgagtc aatccgccag gtcctaaggc
    31741 tgaaagacga cgtttgtgtg tcaattcgcc aagagggttt gtttgatcca tgaactgtga
    31801 caactgagaa gaaccaaaga attctttgat acttgccaca actggacgga tattgatcaa
    31861 ttgttgaggt gtcaatgttt cagtatcttg gatcgacatt ctttcacgaa ccacacgttc
    31921 catacgtgct agcccgatac ggaattggtt ttgtaaaagt tcaccgactg aacggatacg
    31981 acgatttcct aagtggtcga tatcatcgac attgccgata ccttccatca agttcaagaa
    32041 atagctcatt gaagcaatga tgtcggctgg acgaaccgtt tttacagctg cttctggata
    32101 gccgtttccg atcacgttta cttcacgttc agggtctctt ggtgaaaata ctttgatgac
    32161 ttggacagtc attggatctg tgaccacacc gtcttctgat ggatagtaag tcactgaatt
    32221 taggccatta tcaatgaaag gagctagtgt ttccatgact tggtgagtca gaactgttcc
    32281 tttttcaacg atgatctcgc ctgtttctgg atcaactaat gtttcagcta atgtcagatt
    32341 caatagacgt gttttcaaat ctaatttttt gtttactttg taacgaccaa cgtttgctaa
    32401 gtcgtaacgt tttggatcga agaaacgagc attcagcaag ttacgtgagc tgtctgctgt
    32461 ttttggttcg cctggacgca aacgttcgta aacgtctttc aaaccttctt ctgttcttga
    32521 gtcgcttgcg tttttatgca agtctttttc gactgtattg cgtaatgttt cgctgtcgcc
    32581 gaagatttca aagatcgtat catcggaacc aaatcctaac gcacgaacta atacggttaa
    32641 aggaatctta cgtgtacggt cgatacgtac ataagagatg tcttttgcat cagtttccat
    32701 ttctagccaa gctccacggt tagggataac agtagaaccg aatccttctt ttccgttttt
    32761 gtcgactttt ccatggaaat agacacctgg tgaacggact aactgagaaa cgattactcg
    32821 ctctgccccg ttgatgatga atgttccttg ttctgtcatt aatgggaaat caccgaagaa
    32881 tacttcttgt gctttgattt cgccagtctc gcggtttgtc aaacgcaatg ttacatgtaa
    32941 tggtgcagag tagtttgcat catgcgcacg agcttctgct actgtatact ttggttcttt
    33001 caattcataa tctacaaatt ccaaagatag attgccattg aagtcatcga ttggcaaaat
    33061 gtcttcaaac atttctctaa gtccctcatc taagaaccat tggtaagagt ctgtttgaat
    33121 ttcgatcaaa tttggtaatt ccaacacttc actgattctt gcgaaacttc tacgttcgcg
    33181 atgttttccg tattttacta cgtgtccagc caagctcttc acccctctaa aatgtcattc
    33241 aaaaagacta cccgatcaat tgttacaata atatttcaaa tattattttt aggtaacaac
    33301 agacattttt tgcggctttt aggcaaaaaa aaaccaaaaa tagaacattc tcttgaaacc
    33361 ttacttcgtg agcttctact tttgtttcct tatattaaag ccgaaacgga caatatttcg
    33421 tttctgcctt ttcttcatca gatagttttt gcaacttcat atactacact tattttctat
    33481 aaaagtcaac acttcctggc gtttcttctg ctttttcgca acattttctt aatgttttca
    33541 attttatgtt ttcaattatc tgacaattct taattgacag acctttttat ctcggttata
    33601 ctttagaaaa ttcattttgt ggaggaactt ctatgcgaac caacatcaac caaatcgcac
    33661 caacacaact gaataacttt tactttagct actttagata ggtttgtatt catcatgatg
    33721 gatacaaacg ttaaacgaca gtttgtatct atgatggcta aagaactttg tattgcagca
    33781 aaaagccacg tagacaaaat gctaagtggc tgagcagtga aaatacaaag taaaagtatc
    33841 aagcattcta ctttttcaat ttattttccg acttttcaat ccatttggac attttgtctg
    33901 aatggatttt ttgtttccca aaattaagga ggaaaaagaa atgaaaaaaa atataatcat
    33961 cggattaggc gctgccctgc tactagcttt aggaggttgc gcacaaaaag acaattccgc
    34021 agaggcatca gatggacaaa aaacaattgg cgtattgcaa cttgtacaac ataactcgtt
    34081 agatgctgcc tataaaggat tcaaagaagg attagcagaa gaagggtata aagaaggcga
    34141 taatctaaca atcgattatc aaaatgccca aaacaatcaa gacaacctga aaagtatgag
    34201 tgagaaactc gtcaaagaag atcctgattt gctacttgga atcgccactc cagcagcaca
    34261 atctttatta aatgaaacaa ccactatccc gatgactgtc acagcggtca cagacttgga
    34321 acaagcccat ttagtcacat ccaatgaaaa acctggcggc aatgtgactg gcacgacaga
    34381 tatggtgcca atcgaaaaac aaatcggctt actgttaaat atcgtgccag acgccaaaac
    34441 aatcggcatc atgtataaca acggtgaagc aaactctaag atccaagctg atttggcaga
    34501 aaaagaattg aaaaaagcgg gcgtcaaagt caaagtactg acagcaaatt ccacaaatga
    34561 tgtccaacaa gtgacgaaaa gtctagctaa agatgtagat ggaatctata ttcctactga
    34621 taacacgttc gcaagtgcgg caagtgttgt cggtgaagtc gcaaaagaaa caaaaacacc
    34681 tgtcgttgct ggcgcagttg atcaagtcaa agacggcggt ctagcaacag taggaatcga
    34741 ctacgaaagc ttgggcaaac aaacagggat catggcagca aaaatcttag acggcaagaa
    34801 tccaagtgaa cttcctgtcg aaacagcgaa tgatttggaa ctgtatgtca ataaagacat
    34861 ggccaaagca ttaggcatca atccagacag catcaaagaa gctgaataaa acacaggagg
    34921 attctcatga caaatttgct ctctcttttc ttatcatctg catcgcaagg ggtcttatgg
    34981 gccctacttg cgattggtgt atacgtcact ttccgtatct tggacattgc cgacttgact
    35041 gctgaaggaa gctttccttt aggtgcaggg atcgcggcag taagtattac aaatggctat
    35101 catccattga cagcttgtct tctaggcttt ttagggggtg cggcagcagg gcttgtttcc
    35161 ggactgcttc acacaaaatt gaaaattccc gcccttcttg cagggatcat caccatgact
    35221 ggtttatatt ctgtcacatc ccgtgtgatg ggtgcgccga acatctcttt attgggtcag
    35281 ccaacgatct ttcattgggt cgagtcattc ggcataacaa aagacaacgc tgtactgatc
    35341 gtcggtctgc tttttgcttt gatcgttgta tgtttacttg ttttgttctt acgtacagaa
    35401 acaggacttg ctattcgtgc aactggcgat aatctggcaa tgagcgaagc aaacgggatc
    35461 aacacagata atatgaagat catcggttat atgatttcta atggatgcat tgctctttct
    35521 ggctccttac ttgcgcaaaa caacggcttt gctgatttaa attctggtat tggaacgatc
    35581 gtaattggac ttgcttctat tatcatcgca gaagttttgt tacgtaacca gccactattt
    35641 ctccgtctct tgactgtcgt tttaggtgcg gtcatttatc gttttatttt agcgttagtc
    35701 ttcgaactga atgttgaacc aagtgattcg aaacttgctt ctgctttcgt gctagtgatc
    35761 tgtctttctt tcccgcagat ccagcaaaaa ttccatttac ctcaaaaaaa gaaaggcggt
    35821 agtgtcaaat gaatgacgtc ttaacattgg aaaatctcca tcataccttt gaaaaaggaa
    35881 cgatcaacga aaatcatgtc ctgaaaggca ttaatctttc cttagatccc ggcgacttcg
    35941 tgacgatcat cggtggaaat ggggctggta aatctacttt gctgaacagc atcgccggta
    36001 cattttctgt cgatcagggt caaattttat tgaatggaaa aaatatcacg aaaaaatctg
    36061 ttgtcgaacg ttcgaaatcc atcagtcgcg tattccaaga tcctaaacta gggacagccg
    36121 ttcgtttaac ggtagaagaa aatcttgctc ttgctatgaa acgaggaaaa aaacgcggct
    36181 tcttccgtgg tgtcaaacca caagaccgaa catttttcaa agaccagctg gttcggctaa
    36241 atttggggtt ggaaaatcgt ttatcctcag aaatcggttt gctgtctgga ggacaacgtc
    36301 aagctattac gttattgatg gccacattac agcgtccaga actgatctta ttagatgaac
    36361 acactgcagc gcttgaccct gctacttcca aaacggtgat ggaattgaca gaaaagctga
    36421 tcactgagca gaatctcact gcttttatgg tcactcatga catggaagac gcgatccgct
    36481 atgggagtcg actgatcatg ctgcatcaag gaaggatcgt cgtagatatc cagcaagagg
    36541 aaaaacagca gctcacagtg ccccaattat tagcgttgtt caaacaaaac agcggcacgg
    36601 cattaaaaga cgatcaagta cttttatctt cataatcaga aagaggatgg gacaaaaaat
    36661 gaacgcccac cctcttcttt tttctctttc cttctttcgt ttttgcttta tgtatcaacc
    36721 gttgtttctt cgaaaactat tttgttctct ccttcttcct gatagaatgg atgggaaagg
    36781 agcttgatgc catgacaacg aaagacaagg tacttcaatt attgaaaaag acaccagatt
    36841 ttctttccgg agaaaagttg gcacagcaac tggaagtttc ccggacaagt atttggaaag
    36901 ccatcaaaga attggaaaag gcaggctatc gttttgagca cgggcctaca gggtatcgtt
    36961 acctgccctc tgacgtacta gatgcaaaag aaatccagca agagctacgg tcgtttattc
    37021 ctgatttgaa agtacaaacg atgttgactt ctgaatctac gatgaaagat gccaaactgg
    37081 cagcagctca aggagattct gtttcgaagt tatttgttgc tgatacacaa gaacaagctc
    37141 gtggacgttt tggacggcct tttttcgctg aacctggacg aggaatctac atgagtctct
    37201 tgctccatcc agacaaacgg tttgatgaat tgcctcaata tacgatcatc gcagccagtg
    37261 cctgtgtgaa agccattgaa tctgtaaccg gtcgttctgc ttcgatcaaa tgggtcaatg
    37321 atatctacat ggatggaaaa aaagtctgtg ggattttatc tgaagcagtc agtgatatgg
    37381 aaagcggcag gatcagtcat atcatcattg ggatcgggct taatttttct gttcctcaaa
    37441 atcattttcc agctgatctc caagaaaaag ccatctctgt ctttcccgac ggacaagcta
    37501 gtgtcacaag gaatcagcta atcagcaaaa tctggcaaga attgttcact tatctttctc
    37561 acccagaaaa agcgttggac ttctataaag aaaaatcatt cgtattggga aaaaccgtgt
    37621 cgttcaatca aaaaggaacg acttatgtcg gtcttgcaaa agaaatcggt gatcatggcg
    37681 aactgatcgt ccaaacaaat gaacagaaga tgagtttgtc ttctggcgaa atcagtctca
    37741 catctatccg tacgcaataa ttatcagatt tcacaaaagg acacctgatc tcatacagtg
    37801 tccttttttc tgaattctct tcaaaagact ctccttattc tatcagctga cagtttctct
    37861 atcctctaga aaaaggtatt ctaaaaatat ctataaagca tggtacactt tctttaaatg
    37921 aatcaatgca aaaaacaaga gagtcatcag tttacacgat caaaagagat agcaggccgc
    37981 ttcctttttc aatcttatag atagaccatg ggtaacgata aaaacaaaaa agaacttgcg
    38041 acttattttg ccgtaagttc ttttttagct agtgccttaa tctcttaatc ccaacaaacg
    38101 tctcgcatag atcacctgac cagagtgcat agcagcatca tcaataatag aaacgagacg
    38161 cattcctcgt gtgactggtg gtgtccagtt ctcgtcaatt acttcatcta gctcggaagg
    38221 ttccagcctt cttaagtaag aaatagcaaa atcagcagct cctcgcaagt atcctagcag
    38281 atcttctatt tgattagctt ggatctgctt tgcttgttcc agactatgct tccaatcttg
    38341 ttcgttatct gctatcgtca tcggaaaccg gctttcccag tcttgactat gccaaattgg
    38401 agaagttcct gctaaatcag caatctggaa atctaattct cgagcggtat gccaagccag
    38461 ccaagtcatt gatttgatct gcggggctct atcttccact ggaaaacgat tggcctcctc
    38521 aatagagacg ccttcaaaca aacgttcaaa ccgatcgtat gcccgatcca cactctcgca
    38581 tagcatatct aaataatcca tttattttcc tcctttttat ttatcctact tactttttcc
    38641 taattctagg atacctactt atatgttact acacaccctt attttttaca ctttaacagt
    38701 caccatcatc aatagtttgg agattgaaag aaaaagatag tgtcgaacta tcaattgaac
    38761 aagaagcaac aatatgaacg tttcattaat attctttaaa taaacaatga tttttcaaac
    38821 gaacagttta agactaggag aaaaagagta taataggaaa atgtttgaaa ggaaaggaag
    38881 gggaaaaatg aaaaaaaggg caagtttact agtcctagtt gctttgttgt tcaatatctt
    38941 ttcgtatggt gtggccgttt cagcggaaac atacattcct aaagaccctg ggaatattta
    39001 taatgatcta actggtaact atggcttgtt aggaattgct agtcaattcc acgtatttgc
    39061 taaaaataag acaactgtga atgcacatac agacggaaat atagcaacac atgaacttga
    39121 tgcaaaaaat aattttggta cagagcttca tgaaggttta ttgaaccaag aaataaatta
    39181 tatacaaaaa actgattctt tcgctaattc ttcaggcata cctacaggta gcacaagaat
    39241 gaataaattt gtcgttggca gtaacgtgga tgtagcactt caagatggtc atcctacagt
    39301 caatggatcc aggcttgatc acttaactcc agatgatttt tataaagatc gtgctgggca
    39361 tgaatatatt gatttcgagc aggaatttgc taaattgaat gtagcagcaa atgatcttgc
    39421 aacgatcact ccagctaaaa catatactgc tgcagatttt ccagacatga acaatcggac
    39481 aatcgattta actgatttat cagataccgg gtttttattg gtaaatatcg atgcagaagt
    39541 gttaacgatg aatacgccac ttcaaatcat taatccaaat gatcaagtag ttgtatttaa
    39601 tgtcatcaac agttcttctg cattgaacgt acagtcacca atcaaataca atgatcgttc
    39661 aaatcatgaa acagaagatt tttcggatgc gaatatctct tggaattttg gaaatgagat
    39721 gactgatttg acaatcagtg ctccttttca aggaacaatc cttgcgccta atgctactat
    39781 ccgcattact caaaaccaag acggaacgat tataggaaca aatgtgattt tagatgcagc
    39841 taccaatcgt tgggatccta acgaaatatt tatcagcgac aatggtacag atacgacaga
    39901 cacaagcgac agtacagata catctaccac aagtgatagc tcagattcat caacttcaag
    39961 cgacagtaca gatactacct ctaccacaag tgatagcacg gatacatcag cttcaagcga
    40021 cagtacagat actacctcta ccacaagtga tagcacggac acatcagctt caagtgacag
    40081 tacagatact acctctacca caagtgacag cacggacacg tcagcttcaa gcgacagtac
    40141 agatactacc tctaccacaa gtgatagcac ggacacatca gcttcaagtg acagtacaga
    40201 tactacctct accacaagtg atagcacgga cacgtcagct tcaagcgaca gtacggatac
    40261 atctaccaca acaagtgata gcacggacac gtcagcttca agtgacagta cagatactac
    40321 ctctaccaca agtgatagct cagattcatc taccactaca agtgatagca cggacacgtc
    40381 agcttcaagt gacagtacag atacatcctc taccacaagt gatagctcag attcatcaac
    40441 ttcaagcgat agtacagaca cgtcgtctac cacaagcgat acacgtcatg caagtgtctc
    40501 cttctcaaaa acaagttcgg ataacaggac gaactttaac ttaactaata aaacaagcaa
    40561 tgacaatgga aacaaatcaa aaagagcact tcctaaaact ggaagtcaat cgaataattg
    40621 gataacactc gcaggcgtta tattacttgt aatcggttta agaatcactt ttatgagtta
    40681 taagaaatcg aaaaggtaaa aaaatattca agataaaaaa gagcaacgtc gctaacggac
    40741 gttgctcttt atttttgctt tttatcataa atacaagaac tacaattcaa tttgtcaaaa
    40801 aacacgtaga tttccttctg agaaatttat ttacgtatcg aacctttccc tattcagtct
    40861 ttgcgatcca aaatcagttg ggtaagggaa aagagattct cttttgttcg aagagaggtg
    40921 gcaggtaatt tctttatttc cttcaaagcc ttttctgtgt aagaggccgc taacttccta
    40981 gcttcttcca cgcctcctgt atgttcaacg attttgcgta cggcgtcgac atcttcttct
    41041 gtcattgccg ctttcttctc taaataaggt agtagctttg gtttgttttt ttgcaaagag
    41101 tagatcaacg gtaaagaata gaccccttga cgcatgtctt ctagtacagg tttgccgatt
    41161 tcctggctct cttttgtata atctaaaata tcatcgataa tctgaaaggc catcccgata
    41221 gaaagtccga tatctcctgc tttttttgcg aagcgctcgc ttgttccact ttcaaaagcg
    41281 ccaacagaac aacttaattg gaacagttca gctgtcttac ctgaaatatt ttccaaatat
    41341 tcttttacag aaagttcata attatagcga tcatccatct gccctaattc gccattcaat
    41401 actttttcca tactgcgaga attcaattgg agacttttca aggaagtgga gtaatcagac
    41461 aatagtttaa aacagcagac aaataaatag tctcctgcat agacagaaac gacattacca
    41521 aactgactac ggatattagg ctgaccacgt cgcagatccg cttcatcaac gatatcatca
    41581 tggatcagtg tggccgtatg gagcatttcg atagaagctg ccaaagcgat ggctttgtct
    41641 cgatcttgct ctggtccaaa ttgtgagaac aatagctgat aggcaggacg caacagcttt
    41701 ccgccggcat ggatcgtttc tttgatggct tgttcgacag gtttgttttt caggcggaca
    41761 gtttcttcca tccgttttag tgtttctgtc aattctttac ttaaagcagg atagttttcc
    41821 cacattggat gtagcttcat aaaaaaactc cttgtgtgac gattattttt tggtaaacat
    41881 tgggctccat ttttgtttgg ttccctgctc ataatctttt tggaatcgtt gaagtgtctg
    41941 cacatgtcgt aaaagataat tcgctgcgat cccgattgca atccctgata aaatccctaa
    42001 aactgaaagg actggcaagt acaacatgac tgtccatgat tgtgcaatga aagaagccat
    42061 caaaagctgt ccgacattat gcatgaaacc gccggccgca ctaattccga tgatactgac
    42121 acgttttggt cctaattgct tgaccaataa cattccaaaa taactaagca aagcaccact
    42181 catactataa aggaatgtgg aaatcgttcc gcctagcaaa gtagtcagga atagtcgtag
    42241 ccaaagtagt aagaagctgt cttgtttttt catggtaaaa atggcaacga tcgtgattag
    42301 attggctaag cctagttttg caccaggcgc aaaagaaaaa ggataaggga tcatattttc
    42361 caataaacca atgatcactc cttgtgcaac aagtagcgaa atataaatca ttttttgtaa
    42421 tttactcatg gtgtgtgaag gtctaataaa tcagactgtc ttcgtcacct ccatctgttg
    42481 aacggacttc gatcactaat ttatgtggca aacagacgat ggtttcgcca tttttcgttg
    42541 cccagcctcg acgtacgcag atctggtcat cgcaatctgc ttcttttatc cgtactcgat
    42601 cgccatctat ctcgatcaag ttataatctc cgtgttcatc tttgtaagta tatgtgttgc
    42661 tctcttctcc agccatcaaa gggaatgttt tgatttctgt gccatccaca cgcagtaccg
    42721 cttctttttc tgctgttgcg gtgttttgct gaagaccaaa aatcatgact ggtaaaaaag
    42781 aaagaacagt caaaagcaaa ataataaaaa catcccaagg tttgagcaga cttttttcag
    42841 caaattcttt aaggctcatt tttgttctcc tttttccaga ttaactcacc caattttagc
    42901 ataaaaacaa gaggttgtga cacctgtgtc ttgctttgag taataagaac gaagaataga
    42961 aaacagcttt ccatgttttc taattctttg ggcttattac cgagaagcag caggtggaac
    43021 accgtttata acaagaggtt gtgacagaag tgggcaactt caagaaataa gaaggaattt
    43081 acgaaaatta cttttcaagt ttttgtgaaa tttcggctta tttttcgaag aagctacttc
    43141 tggaacaccg tttataacaa gaggttgtaa cagaggttgt gacacctgtg tcttgctcat
    43201 ggaggcttat ctcgggcatc ttattccttt aattttacat aaaaaggagg ctgtgacggt
    43261 ttgtctcagc ccctttctat tgaaatcttg cattcaaata acgttcttta tctatttatt
    43321 tcttgccgta ctgggaactt ggtctaccgt accaccaatt acctagtgta cgttgtagcc
    43381 atggaattac ccagcgatcc aacccaatcg cacgtccgga accgttcatc aatgcgaaag
    43441 caacaaaa
//
LOCUS       DAYYJC010000014        41165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000014 DAYYJC010000000
VERSION     DAYYJC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.014"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1233)
                     /locus_tag="ACHA9N_000727"
     CDS             complement(451..1233)
                     /locus_tag="ACHA9N_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084213.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="ACHA9N_000728"
     CDS             complement(1298..2134)
                     /locus_tag="ACHA9N_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9084214.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="ACHA9N_000729"
     CDS             complement(2152..3561)
                     /locus_tag="ACHA9N_000729"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9084215.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="ACHA9N_000730"
     CDS             complement(3575..5398)
                     /locus_tag="ACHA9N_000730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9084216.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="ACHA9N_000731"
     CDS             complement(6241..6567)
                     /locus_tag="ACHA9N_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084217.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="ACHA9N_000732"
     CDS             complement(6613..6975)
                     /locus_tag="ACHA9N_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9084218.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="ACHA9N_000733"
     CDS             complement(7012..7227)
                     /locus_tag="ACHA9N_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084219.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="ACHA9N_000734"
     CDS             7354..7689
                     /locus_tag="ACHA9N_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084220.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="ACHA9N_000735"
     CDS             complement(7765..9762)
                     /locus_tag="ACHA9N_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084221.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="ACHA9N_000736"
     CDS             complement(9860..10135)
                     /locus_tag="ACHA9N_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084222.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="ACHA9N_000737"
     CDS             10367..11101
                     /locus_tag="ACHA9N_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084223.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="ACHA9N_000738"
     CDS             11140..12282
                     /locus_tag="ACHA9N_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084224.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHA9N_000739"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="ACHA9N_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9084225.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHA9N_000740"
     CDS             complement(13773..14243)
                     /gene="sufU"
                     /locus_tag="ACHA9N_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9084226.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="ACHA9N_000741"
     CDS             complement(14230..15465)
                     /locus_tag="ACHA9N_000741"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9084227.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHA9N_000742"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="ACHA9N_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9084228.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHA9N_000743"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="ACHA9N_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9084229.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="ACHA9N_000744"
     CDS             complement(17731..18579)
                     /locus_tag="ACHA9N_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9084230.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="ACHA9N_000745"
     CDS             complement(18603..19289)
                     /locus_tag="ACHA9N_000745"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9084231.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="ACHA9N_000746"
     CDS             complement(19282..20316)
                     /locus_tag="ACHA9N_000746"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9084232.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="ACHA9N_000747"
     CDS             complement(20711..21049)
                     /locus_tag="ACHA9N_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9084233.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="ACHA9N_000748"
     CDS             complement(21051..21407)
                     /locus_tag="ACHA9N_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9084234.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="ACHA9N_000749"
     CDS             complement(21413..22600)
                     /locus_tag="ACHA9N_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9084235.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="ACHA9N_000750"
     CDS             complement(22791..23636)
                     /locus_tag="ACHA9N_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9084236.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="ACHA9N_000751"
     CDS             23870..24835
                     /locus_tag="ACHA9N_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9084237.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="ACHA9N_000752"
     CDS             25212..26255
                     /locus_tag="ACHA9N_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9084238.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="ACHA9N_000753"
     CDS             complement(26317..26781)
                     /locus_tag="ACHA9N_000753"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9084239.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="ACHA9N_000754"
     CDS             27027..27974
                     /locus_tag="ACHA9N_000754"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9084240.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="ACHA9N_000755"
     CDS             complement(28185..30257)
                     /locus_tag="ACHA9N_000755"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9084241.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="ACHA9N_000756"
     CDS             complement(30190..30567)
                     /locus_tag="ACHA9N_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9084242.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31256)
                     /locus_tag="ACHA9N_000757"
     CDS             complement(30696..31256)
                     /locus_tag="ACHA9N_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9084243.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="ACHA9N_000758"
     CDS             31406..32698
                     /locus_tag="ACHA9N_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9084244.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="ACHA9N_000759"
     CDS             complement(32758..33876)
                     /locus_tag="ACHA9N_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9084245.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHA9N_000760"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="ACHA9N_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9084246.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHA9N_000761"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="ACHA9N_000761"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9084247.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="ACHA9N_000762"
     CDS             37082..37681
                     /locus_tag="ACHA9N_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9084248.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHA9N_000763"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="ACHA9N_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9084249.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="ACHA9N_000764"
     CDS             complement(39376..39720)
                     /locus_tag="ACHA9N_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9084250.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..41002)
                     /locus_tag="ACHA9N_000765"
     CDS             complement(39857..41002)
                     /locus_tag="ACHA9N_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9084251.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13472 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttctt
//
LOCUS       DAYYJC010000015        41057 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000015 DAYYJC010000000
VERSION     DAYYJC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.015"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1468
                     /locus_tag="ACHA9N_000766"
     CDS             128..1468
                     /locus_tag="ACHA9N_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9084252.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1686..2129
                     /locus_tag="ACHA9N_000767"
     CDS             1686..2129
                     /locus_tag="ACHA9N_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9084253.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2633..4051)
                     /gene="nhaC"
                     /locus_tag="ACHA9N_000768"
     CDS             complement(2633..4051)
                     /gene="nhaC"
                     /locus_tag="ACHA9N_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9084254.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4454..5677)
                     /locus_tag="ACHA9N_000769"
     CDS             complement(4454..5677)
                     /locus_tag="ACHA9N_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9084255.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5708..6547)
                     /locus_tag="ACHA9N_000770"
     CDS             complement(5708..6547)
                     /locus_tag="ACHA9N_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9084256.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6564..7244)
                     /locus_tag="ACHA9N_000771"
     CDS             complement(6564..7244)
                     /locus_tag="ACHA9N_000771"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9084257.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7246..8319)
                     /locus_tag="ACHA9N_000772"
     CDS             complement(7246..8319)
                     /locus_tag="ACHA9N_000772"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9084258.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8375..8613)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8656..8889)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8966..9637)
                     /locus_tag="ACHA9N_000773"
     CDS             complement(8966..9637)
                     /locus_tag="ACHA9N_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9084259.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9724..12372)
                     /gene="mgtA"
                     /locus_tag="ACHA9N_000774"
     CDS             complement(9724..12372)
                     /gene="mgtA"
                     /locus_tag="ACHA9N_000774"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9084260.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12714..12888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            13061..13498
                     /gene="ndk"
                     /locus_tag="ACHA9N_000775"
     CDS             13061..13498
                     /gene="ndk"
                     /locus_tag="ACHA9N_000775"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9084261.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13520..14386)
                     /locus_tag="ACHA9N_000776"
     CDS             complement(13520..14386)
                     /locus_tag="ACHA9N_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9084262.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14546..16639)
                     /locus_tag="ACHA9N_000777"
     CDS             complement(14546..16639)
                     /locus_tag="ACHA9N_000777"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9084263.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17388..18011)
                     /locus_tag="ACHA9N_000778"
     CDS             complement(17388..18011)
                     /locus_tag="ACHA9N_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084264.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18170..19567)
                     /locus_tag="ACHA9N_000779"
     CDS             complement(18170..19567)
                     /locus_tag="ACHA9N_000779"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9084265.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19599..20570)
                     /locus_tag="ACHA9N_000780"
     CDS             complement(19599..20570)
                     /locus_tag="ACHA9N_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084266.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20944..21369
                     /locus_tag="ACHA9N_000781"
     CDS             20944..21369
                     /locus_tag="ACHA9N_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084267.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21857..22558)
                     /locus_tag="ACHA9N_000782"
     CDS             complement(21857..22558)
                     /locus_tag="ACHA9N_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9084268.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22574..23014)
                     /locus_tag="ACHA9N_000783"
     CDS             complement(22574..23014)
                     /locus_tag="ACHA9N_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9084269.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(23014..23589)
                     /locus_tag="ACHA9N_000784"
     CDS             complement(23014..23589)
                     /locus_tag="ACHA9N_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9084270.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23830..24039
                     /locus_tag="ACHA9N_000785"
     CDS             23830..24039
                     /locus_tag="ACHA9N_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9084271.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(24437..24949)
                     /locus_tag="ACHA9N_000786"
                     /pseudo
     CDS             complement(24437..24949)
                     /locus_tag="ACHA9N_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25075..25776)
                     /locus_tag="ACHA9N_000787"
     CDS             complement(25075..25776)
                     /locus_tag="ACHA9N_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084272.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25884..27327)
                     /locus_tag="ACHA9N_000788"
                     /pseudo
     CDS             complement(25884..27327)
                     /locus_tag="ACHA9N_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(27341..29206)
                     /locus_tag="ACHA9N_000789"
     CDS             complement(27341..29206)
                     /locus_tag="ACHA9N_000789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9084273.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29327..30178)
                     /gene="licT"
                     /locus_tag="ACHA9N_000790"
     CDS             complement(29327..30178)
                     /gene="licT"
                     /locus_tag="ACHA9N_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9084274.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30488..31201)
                     /locus_tag="ACHA9N_000791"
     CDS             complement(30488..31201)
                     /locus_tag="ACHA9N_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9084275.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31269..31595)
                     /locus_tag="ACHA9N_000792"
     CDS             complement(31269..31595)
                     /locus_tag="ACHA9N_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9084276.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31718..31945)
                     /locus_tag="ACHA9N_000793"
     CDS             complement(31718..31945)
                     /locus_tag="ACHA9N_000793"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9084277.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32267..33520)
                     /locus_tag="ACHA9N_000794"
     CDS             complement(32267..33520)
                     /locus_tag="ACHA9N_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9084278.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33581..34321
                     /locus_tag="ACHA9N_000795"
     CDS             33581..34321
                     /locus_tag="ACHA9N_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9084279.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34407..35165)
                     /locus_tag="ACHA9N_000796"
     CDS             complement(34407..35165)
                     /locus_tag="ACHA9N_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9084280.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35162..35626)
                     /locus_tag="ACHA9N_000797"
                     /pseudo
     CDS             complement(35162..35626)
                     /locus_tag="ACHA9N_000797"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(35645..36142)
                     /locus_tag="ACHA9N_000798"
     CDS             complement(35645..36142)
                     /locus_tag="ACHA9N_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9084281.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(36210..39377)
                     /locus_tag="ACHA9N_000799"
     CDS             complement(36210..39377)
                     /locus_tag="ACHA9N_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9084282.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39601..40485)
                     /locus_tag="ACHA9N_000800"
     CDS             complement(39601..40485)
                     /locus_tag="ACHA9N_000800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9084283.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40580..41017)
                     /locus_tag="ACHA9N_000801"
     CDS             complement(40580..41017)
                     /locus_tag="ACHA9N_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084284.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        11740 a         8633 c         6987 g        13697 t
ORIGIN      
        1 aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta tcattagtca
       61 ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta aaagaaagag
      121 gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata tcaccatgtt
      181 agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag cgattgggat
      241 cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat atatcgtgat
      301 gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat tatctggttt
      361 gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct actggtctat
      421 gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc agttgtggtt
      481 cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta cgatcatcaa
      541 tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg ctaaaatcag
      601 tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa tgttgggcac
      661 aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc ctcatggtat
      721 gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt ctgaactgat
      781 tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag caatcaaagg
      841 tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt tgattctttt
      901 tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat ttgaaaaaat
      961 gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt tgttctcatc
     1021 tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga aggatcgaaa
     1081 aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac gtgctgtttt
     1141 attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg gtgacaaatt
     1201 atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga tcattatcag
     1261 tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag gaatcagctt
     1321 ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga actccattgg
     1381 ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc aaaaaaagaa
     1441 tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt atttatgtgg
     1501 aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa ctcctttttc
     1561 cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct gtgcaaaata
     1621 attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa aggagtttgt
     1681 tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg gtagttatgt
     1741 ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt cgtacacgag
     1801 tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg ttcatgtgga
     1861 aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt cttgtttttg
     1921 ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct tggatgaagg
     1981 ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat ttaaaagatc
     2041 ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag atcgacaaaa
     2101 tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa aaagaggatg
     2161 ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg ccgtgaataa
     2221 ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagcttc
     2281 tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     2341 taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg gaaaaatagt
     2401 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
     2461 tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt gtgaaatggc
     2521 tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg aataccgttt
     2581 attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt ttttattgtt
     2641 ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt gagattcccc
     2701 aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa aatacgaatg
     2761 gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg gcaattcccc
     2821 aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct aatacagttg
     2881 gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga ataacagata
     2941 ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt gtaaaaatca
     3001 aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag atcaagccaa
     3061 attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca gtaggcatca
     3121 tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca gtatttgaaa
     3181 caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg ttttgaataa
     3241 aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc ggcacctttt
     3301 tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa atagtaaagt
     3361 ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct ttacttgtgt
     3421 gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt gaccacatca
     3481 tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga tttgttgatt
     3541 cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct ccaacaacca
     3601 gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca
     3661 taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca aatacagaag
     3721 ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt ggaatgatcc
     3781 ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag attgggatga
     3841 ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg tatcttttta
     3901 tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta ggtgagacct
     3961 ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca agtgttgctt
     4021 ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta gttgttctta
     4081 tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg attcctcctt
     4141 tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt accaccttaa
     4201 ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga ctttccatgt
     4261 aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa caaaacacct
     4321 aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag atcatttgtc
     4381 aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact ttcacacaca
     4441 agtttatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact atttcttgat
     4501 gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga gctgtattgg
     4561 cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta gcagcagctt
     4621 tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta ggctggattg
     4681 ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt ctttgttccg
     4741 tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat aagtcatcaa
     4801 aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac tcaaaagtcg
     4861 tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat actttatcta
     4921 atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct ggaatgacgt
     4981 tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt ggcaattgca
     5041 agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca tttactcctt
     5101 tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg actggatcat
     5161 tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat aacgctacat
     5221 cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg ttctcgcccc
     5281 cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca atgaattgct
     5341 tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg catgcatgac
     5401 caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg ggtttattgg
     5461 aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct acccaaaacc
     5521 tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag ttacttactt
     5581 caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga tattggatta
     5641 gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc tatgctagtc
     5701 atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct tttgatgttt
     5761 tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt tgatacgttt
     5821 tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt gcatggtcat
     5881 ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct acagctacac
     5941 cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg gaagcatcta
     6001 attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg ggcgcttgtc
     6061 cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga gctctgccgc
     6121 catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca gctacatcct
     6181 tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc gatactaaat
     6241 cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa gaattcaaat
     6301 caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat ttcacgtatt
     6361 ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca tctgtatctt
     6421 ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct gcttttcctt
     6481 ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa cgaataattt
     6541 ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg attactgata
     6601 aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt tacatctgtt
     6661 tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc acctactgct
     6721 ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac ccgaatgata
     6781 ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact ggttcccatc
     6841 gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc gatttgacga
     6901 gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc
     6961 gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat gaaagggatg
     7021 gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt agctagtaat
     7081 ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat cccgccaatg
     7141 aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa ttcatccgga
     7201 atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc ttcctccttc
     7261 gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt ttacaccaga
     7321 tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta aagtaacgat
     7381 caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa tattcgctgt
     7441 tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat ttccaatata
     7501 acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat gggcaatgat
     7561 cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg tcaattcttc
     7621 ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc catcttccat
     7681 aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga tcaggacgat
     7741 tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa gggtcgtttt
     7801 cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat tcgccaaagc
     7861 tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat aagcagatgc
     7921 tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac gttcctgttt
     7981 cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc ggctttccat
     8041 tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca attctttggg
     8101 agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg gtttctgcag
     8161 caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga cgattccata
     8221 aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct ctttttcttt
     8281 ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc cttatctaaa
     8341 aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac
     8401 cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc atactctagc
     8461 aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat tgctcgatgg
     8521 ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct gtcaaaggtt
     8581 cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa
     8641 cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc ggaagtttcc
     8701 ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg gtcgaacagt
     8761 tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc ttttcttact
     8821 tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt tccttttcaa
     8881 cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa agtcaagtac
     8941 gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac atttcaataa
     9001 cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct tcgacggtat
     9061 aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct cgtgaagcag
     9121 ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct aatcctgttc
     9181 gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact tcgtctccga
     9241 atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct gttgctcttt
     9301 tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag tctgccaata
     9361 tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga taaaaatatt
     9421 cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct ttcactaaaa
     9481 tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct tttgggatat
     9541 ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt tctgtgtcaa
     9601 ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc ctttccaaaa
     9661 caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa ccagccttct
     9721 cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg tcacaagcgc
     9781 cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa gtggcatcaa
     9841 gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt
     9901 agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt tcggtgtacg
     9961 cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa
    10021 taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc cgcccactac
    10081 agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata tcgaacttgt
    10141 tggaccaaag taaaccatga attccccaat actagatgca ttccattttt ttggctcgtc
    10201 caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat catagatcag
    10261 attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat
    10321 cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat atttcatgat
    10381 attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca aatctttttc
    10441 taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta cagaaatacc
    10501 gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa atccgacagt
    10561 atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac
    10621 gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt ccgagatttt
    10681 ctcaccggta accaattcat tgatctctag acctacttga cggcacactg aacgtgtaac
    10741 taattcgttg tccccagtca gaacttttac agctacacca tgttctttca gagctttcaa
    10801 cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa tcaaaaccat
    10861 gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct tttgtgcgac
    10921 accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta cggcttcttt
    10981 tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag aaatgttcag
    11041 catttcttca atcgcgcctt ttgtgatcat ttgtgtttta ccagctggat cagcaaccac
    11101 aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt gataattgat
    11161 cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta acaaattttt
    11221 cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat catcttcatt
    11281 tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg tccccgtttt
    11341 atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat ttttgatcac
    11401 tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg
    11461 aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag cttctagcca
    11521 atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg acataaattt
    11581 gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa ttggtttttc
    11641 agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa caatgattcc
    11701 ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt aacttgtttc
    11761 gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc tttcccctgt
    11821 caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat ccgctggtat
    11881 catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca taggaatctc
    11941 tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt tcagtttttc
    12001 agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga cgataaccat
    12061 agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta atacatattc
    12121 cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg tgatgtatgc
    12181 tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat tttcaccgta
    12241 attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg catggaatag
    12301 ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt gtgtatcttt
    12361 ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc gctgcttttg
    12421 gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag aacagataga
    12481 taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat ccatttgttc
    12541 tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg aatagttatt
    12601 tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa
    12661 acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga
    12721 tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct tttagctacc
    12781 ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct cgacgttttt
    12841 gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg gttttccttc
    12901 gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt tcatactttt
    12961 tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca ttttccacct
    13021 cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa cacttgttat
    13081 tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac gttttgaagc
    13141 aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat tagcaaaaga
    13201 acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt acatgacttc
    13261 cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat
    13321 ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag actacgcgct
    13381 gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg taaaagagat
    13441 cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata
    13501 caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt tttttctcta
    13561 aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat tcttggtagt
    13621 ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa tctgccatca
    13681 atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc atatcttcat
    13741 tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc aggttcatta
    13801 aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc attctttttt
    13861 tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga aaatcagcta
    13921 gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct tcaatgattc
    13981 ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt tcgaccatgt
    14041 gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta cggttattgc
    14101 tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt cgtctttttt
    14161 tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct gcaagtgtca
    14221 tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc actgaatttg
    14281 tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg gactgtgtca
    14341 acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata gttctccttt
    14401 ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa atacacgaaa
    14461 aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag aatgttgtct
    14521 aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca acgtctgttc
    14581 ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag acacttgtcg
    14641 aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt ttatagtcat
    14701 attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag agattttcct
    14761 gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct ttggagattt
    14821 cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt
    14881 ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa gcaactactt
    14941 gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta tcatcgattc
    15001 ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca tgattgactg
    15061 tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc tgtgcgttgt
    15121 caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct ttattatctg
    15181 tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg taaataccag
    15241 attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt gccgttcgaa
    15301 cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat
    15361 ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta
    15421 gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc ccataaccta
    15481 acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa cctaagtttt
    15541 tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca gcgcttgtat
    15601 agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct gacccaagtt
    15661 ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata ttttcagcat
    15721 cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc gttgcttgat
    15781 tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc atattaatca
    15841 aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta gcgatcccaa
    15901 atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact tcatcaatcc
    15961 cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt ccgtcataaa
    16021 tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat gttcgctgca
    16081 gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa
    16141 gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata ggtattttct
    16201 tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag tagatgacat
    16261 catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc
    16321 ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata atattcaagt
    16381 acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct ggctttggct
    16441 ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca
    16501 agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag taggcaatga
    16561 tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga ttttggcaca
    16621 attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc ttattttgct
    16681 cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta ttctttctaa
    16741 agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga ttaaactaat
    16801 gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag ccagtttctc
    16861 ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct tatttcttga
    16921 tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca actcctcggc
    16981 cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt ccttgtggct
    17041 cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag ctgaccctcg
    17101 gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg ttcaaaagct
    17161 tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt tttgaaattg
    17221 ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg gtgttcaatc
    17281 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
    17341 tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca ttgtccatag
    17401 gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt ttcttcactt
    17461 cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga ctcgtttttt
    17521 tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc cgctaaacga
    17581 tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt tccatttttc
    17641 aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta
    17701 aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt
    17761 tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt
    17821 ttatactccg cccaccaata agttgagaat tcttttttga aattatctac tgattctttg
    17881 cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat
    17941 gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt
    18001 tttttcctca tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa
    18061 atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca
    18121 aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa
    18181 atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc
    18241 catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg
    18301 accagttatc catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg
    18361 cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca
    18421 tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa
    18481 agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt
    18541 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
    18601 aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc
    18661 ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca
    18721 aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac
    18781 ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
    18841 tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat
    18901 tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg
    18961 ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg
    19021 gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa
    19081 accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg
    19141 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
    19201 aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat
    19261 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
    19321 attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
    19381 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
    19441 gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc
    19501 cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt
    19561 ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata
    19621 ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc
    19681 tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg
    19741 atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt
    19801 ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca
    19861 aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta
    19921 gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg
    19981 gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt
    20041 tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc
    20101 actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata
    20161 ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc
    20221 ccatcgatca catgcttcat caggtattca attttttgtt caattttttc tggctgatcg
    20281 gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag
    20341 acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt
    20401 cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct
    20461 tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact
    20521 gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac
    20581 tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt
    20641 gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct
    20701 ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt
    20761 tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt
    20821 ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca
    20881 tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
    20941 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
    21001 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
    21061 aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg
    21121 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
    21181 cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
    21241 aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca
    21301 acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac
    21361 agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
    21421 aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
    21481 atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg
    21541 cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca
    21601 aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt
    21661 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt
    21721 ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat
    21781 aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc
    21841 gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat
    21901 attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct
    21961 tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag
    22021 gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg
    22081 tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt
    22141 ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag
    22201 caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga
    22261 tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg
    22321 atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt
    22381 tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca
    22441 atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca
    22501 tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac
    22561 aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat
    22621 catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca
    22681 agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca
    22741 cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa
    22801 caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc
    22861 cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga
    22921 ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg
    22981 aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg
    23041 atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat
    23101 tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg
    23161 ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag
    23221 accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg
    23281 gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc
    23341 tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg
    23401 atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat
    23461 cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa
    23521 cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt
    23581 aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt
    23641 ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga cgccactata
    23701 tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa aaataaataa
    23761 cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt cgtcctatat
    23821 gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa cgaaaaaatc
    23881 attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa aaaagccgac
    23941 gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa aatctttttg
    24001 ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa
    24061 ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa
    24121 cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat aactttcttt
    24181 cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat aaggtatacc
    24241 agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca aggagggtgc
    24301 cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg attctttcta
    24361 actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt ttttcttgta
    24421 atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct tcttcctctt
    24481 tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat aaaatggtta
    24541 ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg
    24601 tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctta
    24661 atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca
    24721 atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca
    24781 atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct aagaaataag
    24841 cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat
    24901 agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac
    24961 agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt
    25021 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt
    25081 ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat
    25141 catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt
    25201 tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga
    25261 gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc
    25321 tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt
    25381 gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg
    25441 atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg
    25501 ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt
    25561 gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag
    25621 ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc
    25681 tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc
    25741 tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt
    25801 actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt
    25861 tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta
    25921 taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga
    25981 tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcg
    26041 atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc
    26101 gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct
    26161 tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga
    26221 ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca
    26281 cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct
    26341 tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat gtagtccact
    26401 ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact
    26461 ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc
    26521 gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg
    26581 gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc
    26641 aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg
    26701 aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga
    26761 aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat
    26821 ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag
    26881 ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt
    26941 tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg
    27001 ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa
    27061 catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatcg
    27121 attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca
    27181 ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagtcct
    27241 tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc caccccataa
    27301 aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa
    27361 catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag
    27421 tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt
    27481 caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa
    27541 agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt
    27601 cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg
    27661 caggtgcaac tagttctcct acttctggat caatagcgat accttttccc atcacgccag
    27721 aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt
    27781 ttaaggtatg ccgtgctgaa gtaatcggtt ttcctgcatt cgcatgtttg ttttctaatt
    27841 gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa
    27901 aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat
    27961 taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg
    28021 aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct
    28081 tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg
    28141 tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag
    28201 cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat
    28261 attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga
    28321 atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg
    28381 agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa
    28441 atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt
    28501 gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg gctagaatga
    28561 ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac
    28621 tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa
    28681 ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga
    28741 caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat
    28801 cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg
    28861 ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca
    28921 aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga
    28981 tcgattgata aacatctgct acattactgc caatcactac ttgatactga ccgccacttt
    29041 gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat
    29101 cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc
    29161 ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct
    29221 ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga
    29281 acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc
    29341 tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc
    29401 ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg
    29461 cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt
    29521 cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca
    29581 aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta
    29641 ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa
    29701 aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca
    29761 cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa
    29821 ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat
    29881 gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca
    29941 gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct
    30001 ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg
    30061 aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt
    30121 ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt
    30181 tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga
    30241 attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat
    30301 cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct
    30361 atctagaaaa caaccaaatc agagatgcac ctctttgcgt ttctcttttt ttcagcaaat
    30421 tggtttttcc ttatcgattc atatcctttg gctgttttta atctgtaacg acttatgtcc
    30481 cagtatccta aaatgttttt gtctctagat tgaaagcaga tctcatatca tctgtcgcat
    30541 cttttatcga tgtaacactt tccagttctc cttgtacaac tagtcgcgtg acggcgtcca
    30601 tgtcgccaca agtaattagt gtgacaattt cttttccctc gacttcattc agtaattggg
    30661 tatctgtagg agcaactttt tctttaaaaa atgttttgta tgtatacaca ttttctaaat
    30721 ctgtcaaata gatcttggca cccatctcta aattttctag cggtgtaaac agcaattcag
    30781 ggttcgttgc acgatggctt gctaaggcat aatttccttt tcccatttct tgatctggag
    30841 ataatgttcc tgctccgtaa agtagtgacg tattatccaa gcctttgaaa accggtaaat
    30901 tgattgaaac tgaagggact gcgatactag caatcactgg tagatctgtt cctctcattt
    30961 gcgacctcat gaccccttct gtcgtcattg gttctaccgc atcaaagtca aaactgacat
    31021 ctttatcgtt attctttttc agatcttctt ttgttacatt cgctatagca tatttatctc
    31081 ctgtctctct gacaaaataa tccttgatct gttcattgaa aatcaaagct agtcctacta
    31141 ataacaagag gaacaagagg atattgacta gccatctttt tgcttttcct ttttttttca
    31201 ttttctctcc ctccagactc attttactgt ttttcttttg ctctttccaa cagccgaatg
    31261 acacatttct acaactctgt gtattttttt cgtacacctg cgcttttttc tacaggatat
    31321 ttcaaattat ttttgatcaa tttttcttca atgatctcat ctagatccag gtgaaggttg
    31381 tctgccatca tgtatgaata gatcaataca tctgccagtt cttctttgat tcgttctaat
    31441 tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa gttctaacaa ttcagatgct
    31501 tccaaagaaa tagagatagc caaatctttt tcgttatgaa attgtcgcca attacgttca
    31561 tctctaaact ggttgacttt ttccatacta ttcatttgct tgccttcttc caatcacttc
    31621 ttacttgatt aatcatacca tttttttaca taaaaaatag atgatgcttt taaaaaaact
    31681 cctgttttct tttatttaaa ataaatgtca tagaaaatca ataatttagg tagccaccat
    31741 gtgtatgcag tagcgtgtct tcttcccttg aggctttttg tttttcgata cgttcttctt
    31801 cccaaacaga caacgctact agaatctctc ttaatgattt tccttcttcg gtgagcgagt
    31861 actctacttt aggcgggact tggttatagg cttttcgatg gatactttca tcttgttcta
    31921 actctcttaa ctgctgggtc agcatttttt ttgtgatcgt agggatttgc ttgcttctcc
    31981 acgcctaaaa taagcgatat tgctcttccc tttatctggt gtaagcaaag cagttagcat
    32041 tttgacgatc gtgggttccg cttttcactt cttatctact tagtatcatg aagtatactt
    32101 tcgtactaat actaagtagc ggaagtgtca actactttgt ttaactacat agcaaaagaa
    32161 acagaagggt gacaacggtc atcacgcttc tgtttctttt gaatatgctg tgttgttgtc
    32221 tctatctccg tgtagacttt cctcaaactt atcctttgac agtaagtcag taggctgatt
    32281 tgctctttct agatgccaga tacatttcta caaaaaaata gtgattctgt tttgaaaatt
    32341 cgatttttag ttctgcattt tcgtaaacat ccaacatttt tttcgcactg tatagaccta
    32401 acccgccatt ttttttagag gtttttcctc ttttgaccat ctccgatagc gtcaaattcg
    32461 ttggatttgc cttgttcgct atactaaaac gtgatccctc tttttcttcc agcagattga
    32521 tcgtaatcgg actctgatcg gctacacgat gatcgaatgc gttagaaata aaaatagata
    32581 aacagcgaat aaaaacaacc agatcaatcg ggattgtttt cacttcgaca ggaataacta
    32641 cttggatcgg gattttctcc gattctgctt tttctagaaa ttttaaaaag acacccctga
    32701 cagctgggga tacaatattt gttagctgtt gtatttgcgg tttttcaaaa atagctgcag
    32761 aatgatcttc taatatttct aaaaattcca acgtttcttt tttattttcc tgaagagtaa
    32821 tttttaatga aaacagtaga cttttataat catgtttgaa ttcctctaga tgatggtaat
    32881 attcttcatt tatttgatac agttgaagcg attcctgatg ttgtctttct tttacttgga
    32941 aactgatcaa cataatggaa aaaaacaaag aaaaaaaact gatgactatc gacaaaagag
    33001 atagaagcag ataattagat gagcggaagt gaacacttcc caataccaag taaatcacgc
    33061 cccagcttaa taaaatacaa atacctccaa aatagtgttc agttggataa gtaaaaagca
    33121 agttgagaaa tctcgtctgt ttgtctatac tttttactaa tagatagaaa ataaatgcga
    33181 taagtagtgc aaaaaaactt gctgttccaa gatgtctctt tcctaaataa tccatcagat
    33241 caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa aagattactg atcaaacagc
    33301 tgataaacag gtttttcgta gtaaaataaa aaagaaacat caataataag ccaactaggt
    33361 aaggaaagct cactttaggg ataggaagta aaagtgtcac caccatcaaa gctatacaac
    33421 caaagctgtt ttttactagc agttgtttcg aaaagaataa atagatacct aaatctaaaa
    33481 tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat cttattcctc cattgttttg
    33541 tttcaaaatg tacacatatt tttttaatag gagcctgcat atgaaaaagt attttatcat
    33601 agaagataac cagtttcaca gcgaatggat catcagagaa ttggtcaatc atcatatcga
    33661 gaatatcgtg cagctcactt ctatctctca aatcaatcag ttgatccagt tgggcactat
    33721 tgataatcac gatgttttct ttatcgatat cgacttgaag caatcactca caggtattga
    33781 tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat attgtttttt tcaccgctta
    33841 tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt ccgattggat atatcagaaa
    33901 agacaggaat ttatctgaac aaattcgtca agtgctagaa cagatcaatt ggaaagaaca
    33961 gcagatcgta aatgtcgata aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta
    34021 tcctcgggaa attttatata ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa
    34081 aacaactaca gaagataaat tagtgaatac ttctttaaaa caattgaaga cagagttaga
    34141 aaagtatgct tactttttac ttttaaaatc ttactgtatc aacttatcgc aattgcagaa
    34201 aattgattct gctcattatg tgttagaatt tttcaacggt gactccttac tcgttggtcg
    34261 aaagatcttt gaaaagacaa aggaacgttt ccatgacttc caaaaaactg cttcgagttg
    34321 atgaataaga gctgagacgt aagcatagca gatttcaggg agtgggcctt gaatcggctc
    34381 cactccctga aatcgttgat atgattttac gcttcctctt ctgtaggagc atctgttaac
    34441 gaccatgtga atgttgaaac gtagttgtct acagcataat tggatacagg aacaaataat
    34501 tccactgatt gtctcccttg ttccacattc tctcctaatg aataaatcca tgtgccaatc
    34561 ccttcatttg ctcctgcagt tactagatct actgcctgat ctgctgggat ttcgattcct
    34621 tcagcgccag ttccagaaac ggtagatggc attccactgc tattagttgc gctatttcga
    34681 atcacggcgt ttttgaattt cacctttgaa ccagttagtt tattcgtatc agatgttgta
    34741 ccaataaatt ctgttcgttt gactgataat ttccaaccgg cataatttcc gcttttatcc
    34801 gttacttgta cgaaattctg tacttgtgtt tgtgctccag tactgtccgt tccgctttgt
    34861 aatgcagcat atactgtttt tgctgtacct aacttgattg ttccaaaatc aagatctgga
    34921 gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga
    34981 gtatcaggat catttggatc aacaggagga actggaacaa cgtcttcagg atttgcagca
    35041 tctggtttgg tcacagaaat cgttccttct gtagtggctg agctcttatt ctcagcaaaa
    35101 acactttgtg tacataataa tagacttcca gctagaacgg tacttaatgc aattgttttt
    35161 ttcatttctt tttccttctt tcttttaaaa taaataatcc agcaaataag ccaataagaa
    35221 tgactccaac aagtatcagc catcgccagt caaatgtttc tggctgattt tttaaatcaa
    35281 tatccttttc attcaattgg tccgctgcat catcagttat ttcaaaatcg gtatggagat
    35341 tccagcgttt ttctttccaa gcacccttca caaccgcttg ataatgtccc ggagctaatt
    35401 tttttccttc taatgggaca agtaaatcaa aatttgtatt gggtgctaca tctatttttt
    35461 ctttttttat tgaaaggtaa ggctcttttg tttctttttt gtagatttca accgatgtag
    35521 tcaagccgaa catataagaa gcatttggat tttgaagatt cgccttaatc acatttcttc
    35581 catcgctttg atccgcataa gcttttttta atttcaaatc tggggaagac tctttttcgc
    35641 tatttcaaat caagattgct ttcacttgag aaaactgatt gacgacacca actgtacttt
    35701 tgttagaaac gttctctttt ttttcttgaa acgtgatccc tcctgcgacc acaccagcca
    35761 gctctttttc tggcgctttc agtgaaagtg tctcagtgac cgcactttga ggaggaacag
    35821 ttatttctcc cactgttttt gctaagtttt tcatatcata acttaatgtt tcatcaaatt
    35881 tactttttga ttctccatat tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca
    35941 atgtctcaac tgttactgat ttatctgttc ggttaaagac cgtgtagggg atttcctgtg
    36001 ttttcccagg ctccatcctt acatttatat atgttttgtt cttatcaatc tgattagccg
    36061 gtaattcaaa atctaccgca aaattgaatt cggaggcatg gacaggtgtc tgccaaagga
    36121 tgaatccaag gagaaggatc ataagtaaag agcagttgtt tcgatgactt ttattccata
    36181 ttcgattcat caatcatcct ccttgatctt tattctgtcg gcgcatctgc taatgtccat
    36241 gttatctctg ctgagtaatt accagctatc tgattttctt tggttaagac aagtttaaac
    36301 gcccgaccgt tatttaaata ttctgaataa tctgcttgca cattcgttga attttgtcct
    36361 ctgacaattt ccagttcttc ttcattcact aattgatact cctctccttt cttgaaatat
    36421 aggacatcct gcatagaaaa tcctgcatca tttgtgaaag gctgagataa ttttatcgtt
    36481 aagatccacg gatttttcgt ttctcgctga tcataaatcc ataatttttc ctgtatatcc
    36541 atttggccaa atgcttcttg tgtcaaacca gttgctttga tctgaccaaa atcgagtttg
    36601 gaaggggcaa gaaatgagag accaccttct actacgatct tcgtagcagg tgcaaaaggc
    36661 ggggcatcat gataaacaac tggcgtatca cttggattgt gattcccaat ttcatcgttg
    36721 gtacttggct tgttctgcac acccttagca gtagcttctc ctgcttggtc gtttaataca
    36781 atttctaata catctcctgc aaaaagttga ttttctggga tagaaagaac aaattcccct
    36841 gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt ttttcaatga aaccaaggct
    36901 cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa
    36961 ttagcaggtt caggaggaat cacatcgact gtcgttttgg cagagtccag ttctgtcact
    37021 tcttggccac ctcttacttt tttagcagca acaacttgca cttgatcctc cgtttttaat
    37081 ctctcatcat tgggtacttg aacttcgaac aagccgcctt gttcgccttc tccccatggg
    37141 ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg tgagagcagt aatttctaca
    37201 gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca cttctacttc gcctgtccaa
    37261 gcatctcttg gctttgcctc gacaccttcg ggaacaacga catgcccata gattttttta
    37321 tctgcattcg ttggcactct taaggattca atcatcggga gctgattatt ggcactgatt
    37381 cgattataat ttttcattcc ggcacctgtc tgagcaaatt catttgttaa acctgcacta
    37441 gggcttgata cgaaattgcc taagtcggtc ccattgatcg ataagtctgt catatcagaa
    37501 aaataatccg ggtctttttc aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg
    37561 atttctgaat gggcgatctt taaagaagaa tctccatttc cttggaatac ccagccgcct
    37621 atgccattgg tctcgcttcc aatccgtttg ttttggaaat caaaataggt aggagattct
    37681 aagtccacat tcaacacatc cccactgatg atgccggtct tatcttcccc tgttttcccg
    37741 accatgacta aataagtttt ttcacctgcc gtaaaattta aatcaatatc tccagttgta
    37801 tctacagttg ctccggaatc tgcgcgtata tcgatatttg atttttctcc agtgactgta
    37861 aaatcagctg aacctggact ttctagaaaa ccagaagcgt cataaaattg gatcgcttga
    37921 tttccttgat tgagtctgtt cacgtaagat ttgccatcac ctgtatttct taccctcaat
    37981 gagcctccat tggttactgt cagcttttgc gtaccattct caaaacgaac tgcgctggaa
    38041 cgcccactct gcttatcgat gtcgataact gatccggctc cttctacgat caaagaaagg
    38101 ccttttccta caaaacgcat cccattgttc ccgggttgat cgtcatctcc agccattttg
    38161 accgagattt gtccaccact tgatacatgg atttgactag aatctccttg caagcgaaat
    38221 ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat tcgatacagt gactttcgat
    38281 cctgaacctc tcatttggat gattccgcga ttgttgtcat tggtaatatt gatctttgta
    38341 tttaccgaaa attcgcttcc cgaaccactg acttcaaaca tgacattacc aggtagcgta
    38401 tcgactgcct ctccttcatc gctaacgcca tctgcccgat cataatcaag agagaccttt
    38461 gaaccgctta agatatggat tcctggcgct gtttttccac taccagtctc atctcctgaa
    38521 tctagaaaac cgtagaattt ccctagatac ggtccataaa gttgactacc attgatcaca
    38581 ttcaattgat agcttctaca aaaataggct ttacccggag cggcattcag tccttccttc
    38641 actggttgga catccaacac accggaaaca ccatctaatg tgacagttgc acgaggagcg
    38701 taaatcagtc ctaatttgga cccatcatac aaatcaagtg tcccggtaaa ttttatttct
    38761 cccgtaaaat gatatctgct tccccaaaaa tcggtcatat aagcaatgat aatggcattt
    38821 ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg ttcctttaaa attctggaac
    38881 tcgatcgact gaggtatctc attgatataa atatggtgtg tgccgaaatt gtgagcgaat
    38941 ccaccaaaat caataacgat atctcgtttg atctctaatt ccctgtctaa agtcaaaggc
    39001 tgaatcagtc ggatcttagt tactgattcg tctgcgagaa gctgcgccag ttcttctgtt
    39061 gtggcagctt cgctgtaccc atccgctgaa acagctcttg ccagtgccgc ttcactgcta
    39121 tcagacgttt gttcagctac ttctgaggtc tcacttgtct tattttcttc ttgcctttta
    39181 ttagtagaag cctcactttg agattctgta gtttcagaag taatcgattc ttctgaaaaa
    39241 gtagtcttgt caaaactttg attctctgat tgcgttgatt cgtttgtttt ctctacatcg
    39301 gcaaaaacgg atgtcaccat caagagaggg aagacaaaca aaatcgacgc aatcagcttt
    39361 tttacacatt gattcattct ccaccatcct ctctgacgtt ttgacaattt tattttatcg
    39421 ttttaatcta tttccaattg tactttcata tttaaaatat gtatttttat ggaaaaattt
    39481 ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca
    39541 cagtccctta tcccgaacaa gcggtgttct cccgccaact cctcggtcac aacctccatt
    39601 ttaacaataa acctgtaaat gtgagggcaa tactttgatt gtcacaggta atttatcccc
    39661 ttcatctcca tcaacattcg tcgtcaattc ttcctcatca gctaatgaca caactaattg
    39721 ctcacaagta aggtaggcta gatttttcgt agattcatct actcctttta ataggtctgg
    39781 caatgtttta agcgtatcga tcaaagaaga atcttttaaa taaaccaaat ggagcttacc
    39841 atcatccact tttgcatttg gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc
    39901 tatcagcaag gtactgctct caatttcttc cttctttcca tcaacttcta cgtgaaacga
    39961 gtaggattgt gtggaagcta actgtttgat tcctgacatg aaataagcta gttttccaaa
    40021 tttcgtcttc ttctccgcat ctacatcatt gattgattca gggatagagc caattgcgac
    40081 gacattcata aaatagtcgt catttatttt tccaatatcc aatggcttga ctgattcaat
    40141 cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc cctaatgctc gagccaagtc
    40201 attcaccgta cccaatggga aaaatccgaa atttggccga tgttcttgtt cagcgattcc
    40261 actgattcct tcgttgactg ttccatctcc gcccatgaca aacacactat gatacccttc
    40321 cgtagccgct tcgcgagtaa agttttttgc atcccctgct ttttttgtat gcaagacgac
    40381 cacttcatca aacaccgatt ccaattttgc tatcgctaat tgctcaaatt cctttgcttg
    40441 ttcaccgcct gaactggggt ttaccactaa taaaactttt ttcatcactt tctttccctt
    40501 ctgattgatt tctttaatat aacgatagca taaagaaaaa aattaggctg tgatgaagta
    40561 ctgttcatga aacattcatt tattttttga caagataaaa catccatact atgaaactga
    40621 ggaaaattaa aacaatgata taggaaataa aagaaaatga taggcattgt atacctaata
    40681 aagtaaaaat cttaggtggc agataaaaac acaaaaatag caagagaatg taatagcgtt
    40741 tcttccactt tttgttatca gaccttatag cgatcagagg aataccaaat gcataactga
    40801 aaaactcttg atgtactgtt aatatgtcac ggccttgaat aataaatgcg acaatccccg
    40861 ctataattag tgtgcctaac accaaaacgg aaaattgatg aatatatttt atatacaaat
    40921 gatccattgt acttcctgcc tttttatttg tgagaaaaac aaatccgtat acgtgaataa
    40981 cttctagaaa taaaagaaat ttgcaaaacc actccattta tctacttcta cgtgcatttt
    41041 ttgatattgt tttcttt
//
LOCUS       DAYYJC010000016        40414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000016 DAYYJC010000000
VERSION     DAYYJC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.016"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA9N_000802"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="ACHA9N_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9084285.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA9N_000803"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="ACHA9N_000803"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9084286.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="ACHA9N_000804"
     CDS             complement(2303..2965)
                     /locus_tag="ACHA9N_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084287.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="ACHA9N_000805"
     CDS             3149..4795
                     /locus_tag="ACHA9N_000805"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9084288.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA9N_000806"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="ACHA9N_000806"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9084289.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="ACHA9N_000807"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="ACHA9N_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="ACHA9N_000808"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="ACHA9N_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="ACHA9N_000809"
     CDS             complement(8944..10023)
                     /locus_tag="ACHA9N_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9084290.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="ACHA9N_000810"
     CDS             complement(10126..10566)
                     /locus_tag="ACHA9N_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9084291.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="ACHA9N_000811"
     CDS             complement(10623..11033)
                     /locus_tag="ACHA9N_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9084292.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="ACHA9N_000812"
     CDS             complement(11213..11647)
                     /locus_tag="ACHA9N_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9084293.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="ACHA9N_000813"
     CDS             complement(11747..12595)
                     /locus_tag="ACHA9N_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9084294.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="ACHA9N_000814"
     CDS             12984..14750
                     /locus_tag="ACHA9N_000814"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9084295.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="ACHA9N_000815"
     CDS             14852..16111
                     /locus_tag="ACHA9N_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9084296.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="ACHA9N_000816"
     CDS             16258..17559
                     /locus_tag="ACHA9N_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084297.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="ACHA9N_000817"
     CDS             17562..18413
                     /locus_tag="ACHA9N_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9084298.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="ACHA9N_000818"
     CDS             18426..19241
                     /locus_tag="ACHA9N_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9084299.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="ACHA9N_000819"
     CDS             19392..20558
                     /locus_tag="ACHA9N_000819"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9084300.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="ACHA9N_000820"
     CDS             20788..21435
                     /locus_tag="ACHA9N_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9084301.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="ACHA9N_000821"
     CDS             21519..22463
                     /locus_tag="ACHA9N_000821"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9084302.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="ACHA9N_000822"
     CDS             22598..24049
                     /locus_tag="ACHA9N_000822"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9084303.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="ACHA9N_000823"
     CDS             24070..24906
                     /locus_tag="ACHA9N_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9084304.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="ACHA9N_000824"
     CDS             24906..25565
                     /locus_tag="ACHA9N_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9084305.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="ACHA9N_000825"
     CDS             25691..26380
                     /locus_tag="ACHA9N_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084306.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="ACHA9N_000826"
     CDS             26386..27342
                     /locus_tag="ACHA9N_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9084307.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="ACHA9N_000827"
     CDS             27386..27898
                     /locus_tag="ACHA9N_000827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9084308.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="ACHA9N_000828"
     CDS             complement(28154..28702)
                     /locus_tag="ACHA9N_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9084309.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="ACHA9N_000829"
     CDS             complement(28791..29972)
                     /locus_tag="ACHA9N_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9084310.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="ACHA9N_000830"
     CDS             complement(30208..31716)
                     /locus_tag="ACHA9N_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9084311.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="ACHA9N_000831"
     CDS             31875..32066
                     /locus_tag="ACHA9N_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084312.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="ACHA9N_000832"
     CDS             32269..33186
                     /locus_tag="ACHA9N_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9084313.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="ACHA9N_000833"
     CDS             33179..34252
                     /locus_tag="ACHA9N_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084314.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="ACHA9N_000834"
     CDS             34400..34918
                     /locus_tag="ACHA9N_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084315.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="ACHA9N_000835"
     CDS             34899..35291
                     /locus_tag="ACHA9N_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9084316.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="ACHA9N_000836"
     CDS             35367..36302
                     /locus_tag="ACHA9N_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9084317.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="ACHA9N_000837"
     CDS             36435..37214
                     /locus_tag="ACHA9N_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9084318.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="ACHA9N_000838"
     CDS             37207..37524
                     /locus_tag="ACHA9N_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9084319.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="ACHA9N_000839"
     CDS             37768..39378
                     /locus_tag="ACHA9N_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9084320.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="ACHA9N_000840"
     CDS             39535..40383
                     /locus_tag="ACHA9N_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9084321.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DAYYJC010000017        35398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000017 DAYYJC010000000
VERSION     DAYYJC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.017"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACHA9N_000841"
     CDS             <1..649
                     /locus_tag="ACHA9N_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084322.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="ACHA9N_000842"
     CDS             708..1148
                     /locus_tag="ACHA9N_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084323.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="ACHA9N_000843"
     CDS             1160..1828
                     /locus_tag="ACHA9N_000843"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9084324.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="ACHA9N_000844"
     CDS             1892..2515
                     /locus_tag="ACHA9N_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9084325.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="ACHA9N_000845"
     CDS             2571..2957
                     /locus_tag="ACHA9N_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9084326.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="ACHA9N_000846"
     CDS             3170..4561
                     /locus_tag="ACHA9N_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9084327.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="ACHA9N_000847"
     CDS             5172..5540
                     /locus_tag="ACHA9N_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9084328.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="ACHA9N_000848"
     CDS             5720..6661
                     /locus_tag="ACHA9N_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9084329.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="ACHA9N_000849"
     CDS             6730..7461
                     /locus_tag="ACHA9N_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9084330.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="ACHA9N_000850"
     CDS             7605..9785
                     /locus_tag="ACHA9N_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9084331.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="ACHA9N_000851"
     CDS             9805..10287
                     /locus_tag="ACHA9N_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9084332.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="ACHA9N_000852"
     CDS             10303..11064
                     /locus_tag="ACHA9N_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9084333.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /gene="murC"
                     /locus_tag="ACHA9N_000853"
     CDS             11195..12529
                     /gene="murC"
                     /locus_tag="ACHA9N_000853"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9084334.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="ACHA9N_000854"
     CDS             12836..13321
                     /locus_tag="ACHA9N_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9084335.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="ACHA9N_000855"
     CDS             13314..13997
                     /locus_tag="ACHA9N_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9084336.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="ACHA9N_000856"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="ACHA9N_000856"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9084337.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="ACHA9N_000857"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="ACHA9N_000857"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9084338.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /gene="coaE"
                     /locus_tag="ACHA9N_000858"
     CDS             17613..18242
                     /gene="coaE"
                     /locus_tag="ACHA9N_000858"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9084339.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="ACHA9N_000859"
     CDS             18294..18626
                     /locus_tag="ACHA9N_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9084340.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="ACHA9N_000860"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="ACHA9N_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9084341.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="ACHA9N_000861"
     CDS             19452..20825
                     /locus_tag="ACHA9N_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9084342.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="ACHA9N_000862"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="ACHA9N_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9084343.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="ACHA9N_000863"
     CDS             21880..22320
                     /locus_tag="ACHA9N_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9084344.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="ACHA9N_000864"
     CDS             22464..23165
                     /locus_tag="ACHA9N_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9084345.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="ACHA9N_000865"
     CDS             23162..24283
                     /locus_tag="ACHA9N_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9084346.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="ACHA9N_000866"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="ACHA9N_000866"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9084347.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="ACHA9N_000867"
     CDS             25662..26570
                     /locus_tag="ACHA9N_000867"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9084348.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="ACHA9N_000868"
     CDS             26605..26877
                     /locus_tag="ACHA9N_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9084349.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="ACHA9N_000869"
     CDS             26888..27934
                     /locus_tag="ACHA9N_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9084350.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="ACHA9N_000870"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="ACHA9N_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9084351.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="ACHA9N_000871"
     CDS             complement(30945..31631)
                     /locus_tag="ACHA9N_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084352.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="ACHA9N_000872"
     CDS             complement(31609..32103)
                     /locus_tag="ACHA9N_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9084353.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="ACHA9N_000873"
     CDS             complement(32123..33343)
                     /locus_tag="ACHA9N_000873"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9084354.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="ACHA9N_000874"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="ACHA9N_000874"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9084355.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DAYYJC010000018        34540 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000018 DAYYJC010000000
VERSION     DAYYJC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.018"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHA9N_000875"
     CDS             212..604
                     /locus_tag="ACHA9N_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9084356.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHA9N_000876"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHA9N_000876"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9084357.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHA9N_000877"
     CDS             2698..2943
                     /locus_tag="ACHA9N_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084358.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHA9N_000878"
     CDS             3165..4775
                     /locus_tag="ACHA9N_000878"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9084359.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHA9N_000879"
     CDS             complement(4752..4964)
                     /locus_tag="ACHA9N_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084360.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHA9N_000880"
     CDS             5067..5936
                     /locus_tag="ACHA9N_000880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9084361.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHA9N_000881"
     CDS             6101..7372
                     /locus_tag="ACHA9N_000881"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9084362.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHA9N_000882"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHA9N_000882"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9084363.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHA9N_000883"
     CDS             8763..9026
                     /locus_tag="ACHA9N_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9084364.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHA9N_000884"
     CDS             9471..9920
                     /locus_tag="ACHA9N_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9084365.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHA9N_000885"
     CDS             9977..11395
                     /locus_tag="ACHA9N_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9084366.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHA9N_000886"
     CDS             11395..12255
                     /locus_tag="ACHA9N_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9084367.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHA9N_000887"
     CDS             12429..13202
                     /locus_tag="ACHA9N_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9084368.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHA9N_000888"
     CDS             13349..13714
                     /locus_tag="ACHA9N_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9084369.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHA9N_000889"
     CDS             complement(13862..15070)
                     /locus_tag="ACHA9N_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9084370.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHA9N_000890"
     CDS             complement(15089..15367)
                     /locus_tag="ACHA9N_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084371.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHA9N_000891"
     CDS             15560..16216
                     /locus_tag="ACHA9N_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9084372.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHA9N_000892"
     CDS             complement(16658..17455)
                     /locus_tag="ACHA9N_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9084373.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHA9N_000893"
     CDS             complement(17452..18636)
                     /locus_tag="ACHA9N_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9084374.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHA9N_000894"
     CDS             complement(18661..19908)
                     /locus_tag="ACHA9N_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9084375.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA9N_000895"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHA9N_000895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9084376.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHA9N_000896"
     CDS             21888..22202
                     /locus_tag="ACHA9N_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9084377.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHA9N_000897"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHA9N_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9084378.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA9N_000898"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHA9N_000898"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9084379.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHA9N_000899"
     CDS             23546..23875
                     /locus_tag="ACHA9N_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9084380.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHA9N_000900"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHA9N_000900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9084381.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHA9N_000901"
     CDS             24863..25687
                     /locus_tag="ACHA9N_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9084382.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHA9N_000902"
     CDS             25680..26027
                     /locus_tag="ACHA9N_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9084383.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA9N_000903"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHA9N_000903"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9084384.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA9N_000904"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHA9N_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9084385.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA9N_000905"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHA9N_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9084386.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA9N_000906"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHA9N_000906"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9084387.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHA9N_000907"
     CDS             31684..32451
                     /locus_tag="ACHA9N_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084388.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHA9N_000908"
     CDS             32441..34483
                     /locus_tag="ACHA9N_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084389.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11105 a         6123 c         7253 g        10059 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt agaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg
//
LOCUS       DAYYJC010000019        34387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000019 DAYYJC010000000
VERSION     DAYYJC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.019"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            342..1031
                     /locus_tag="ACHA9N_000909"
     CDS             342..1031
                     /locus_tag="ACHA9N_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9084390.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1018..2226
                     /locus_tag="ACHA9N_000910"
     CDS             1018..2226
                     /locus_tag="ACHA9N_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9084391.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2520..3791
                     /gene="serS"
                     /locus_tag="ACHA9N_000911"
     CDS             2520..3791
                     /gene="serS"
                     /locus_tag="ACHA9N_000911"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9084392.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHA9N_000912"
     CDS             complement(4340..5824)
                     /gene="guaB"
                     /locus_tag="ACHA9N_000912"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9084393.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6329..7024)
                     /locus_tag="ACHA9N_000913"
     CDS             complement(6329..7024)
                     /locus_tag="ACHA9N_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9084394.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHA9N_000914"
     CDS             complement(7114..8214)
                     /gene="ychF"
                     /locus_tag="ACHA9N_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9084395.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8223..8414)
                     /locus_tag="ACHA9N_000915"
     CDS             complement(8223..8414)
                     /locus_tag="ACHA9N_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9084396.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8435..9325)
                     /locus_tag="ACHA9N_000916"
     CDS             complement(8435..9325)
                     /locus_tag="ACHA9N_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9084397.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9312..10079)
                     /locus_tag="ACHA9N_000917"
     CDS             complement(9312..10079)
                     /locus_tag="ACHA9N_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9084398.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHA9N_000918"
     CDS             complement(10091..10804)
                     /gene="rsmG"
                     /locus_tag="ACHA9N_000918"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9084399.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHA9N_000919"
     CDS             complement(11036..12937)
                     /gene="mnmG"
                     /locus_tag="ACHA9N_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9084400.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHA9N_000920"
     CDS             complement(12954..14351)
                     /gene="mnmE"
                     /locus_tag="ACHA9N_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9084401.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14650..15033
                     /locus_tag="ACHA9N_000921"
     CDS             14650..15033
                     /locus_tag="ACHA9N_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084402.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHA9N_000922"
     CDS             complement(15201..15959)
                     /gene="jag"
                     /locus_tag="ACHA9N_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9084403.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15977..16795)
                     /locus_tag="ACHA9N_000923"
     CDS             complement(15977..16795)
                     /locus_tag="ACHA9N_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9084404.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHA9N_000924"
     CDS             complement(16792..17148)
                     /gene="rnpA"
                     /locus_tag="ACHA9N_000924"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9084405.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHA9N_000925"
     CDS             complement(17266..17400)
                     /gene="rpmH"
                     /locus_tag="ACHA9N_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9084406.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHA9N_000926"
     CDS             17923..19257
                     /gene="dnaA"
                     /locus_tag="ACHA9N_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9084407.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHA9N_000927"
     CDS             19455..20585
                     /gene="dnaN"
                     /locus_tag="ACHA9N_000927"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9084408.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20814..21056
                     /gene="yaaA"
                     /locus_tag="ACHA9N_000928"
     CDS             20814..21056
                     /gene="yaaA"
                     /locus_tag="ACHA9N_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9084409.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21043..22167
                     /gene="recF"
                     /locus_tag="ACHA9N_000929"
     CDS             21043..22167
                     /gene="recF"
                     /locus_tag="ACHA9N_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9084410.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22164..24110
                     /gene="gyrB"
                     /locus_tag="ACHA9N_000930"
     CDS             22164..24110
                     /gene="gyrB"
                     /locus_tag="ACHA9N_000930"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9084411.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24130..26601
                     /gene="gyrA"
                     /locus_tag="ACHA9N_000931"
     CDS             24130..26601
                     /gene="gyrA"
                     /locus_tag="ACHA9N_000931"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9084412.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26837..27136
                     /gene="rpsF"
                     /locus_tag="ACHA9N_000932"
     CDS             26837..27136
                     /gene="rpsF"
                     /locus_tag="ACHA9N_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9084413.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27186..27698
                     /gene="ssb"
                     /locus_tag="ACHA9N_000933"
     CDS             27186..27698
                     /gene="ssb"
                     /locus_tag="ACHA9N_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9084414.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27724..27960
                     /gene="rpsR"
                     /locus_tag="ACHA9N_000934"
     CDS             27724..27960
                     /gene="rpsR"
                     /locus_tag="ACHA9N_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9084415.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28103..30076
                     /locus_tag="ACHA9N_000935"
     CDS             28103..30076
                     /locus_tag="ACHA9N_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9084416.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30082..30534
                     /gene="rplI"
                     /locus_tag="ACHA9N_000936"
     CDS             30082..30534
                     /gene="rplI"
                     /locus_tag="ACHA9N_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9084417.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30808..32175
                     /gene="dnaB"
                     /locus_tag="ACHA9N_000937"
     CDS             30808..32175
                     /gene="dnaB"
                     /locus_tag="ACHA9N_000937"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9084418.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32955..34247
                     /locus_tag="ACHA9N_000938"
     CDS             32955..34247
                     /locus_tag="ACHA9N_000938"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9084419.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10492 a         6689 c         6807 g        10399 t
ORIGIN      
        1 gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa caaataataa
       61 tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac atttctagct
      121 tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc cgagagacgt
      181 aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac caactagatg
      241 tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct gctttttttt
      301 gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt ttgattgctg
      361 acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag gggtatgaag
      421 cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc cctgatattg
      481 aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta gtgaaagagc
      541 tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact gatatggata
      601 agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt aatccgttag
      661 aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt gccccagatc
      721 agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat gaagttaaaa
      781 caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc tacttgcttg
      841 ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt tggaaacaag
      901 aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga gataagattg
      961 aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc tataaaattg
     1021 acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac ttcaaaagaa
     1081 atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct attaaatgtt
     1141 tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac gaatgcgatt
     1201 tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg gaacggccgg
     1261 aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg gcgcttgatt
     1321 cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta catcgcaaac
     1381 ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg agtggtaaca
     1441 agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg taaaatcgaa
     1501 aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc actcacgtcc
     1561 atcattggat acctaggact gatagaagat ggccagtatc actcagaaga agatctttta
     1621 aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt ggacgactta
     1681 tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc ctttgacatg
     1741 atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa aaaaaatatc
     1801 caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac cgaaaaactt
     1861 gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg agcaacgaag
     1921 atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa aaataacgga
     1981 gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg tgtagaagaa
     2041 tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca aagcattgtg
     2101 gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc tttcatcatg
     2161 caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt tattgacgaa
     2221 agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac acagaaccca
     2281 gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa ggataatttg
     2341 gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca acagccgtta
     2401 tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt cagactgatt
     2461 cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga ggaaatcata
     2521 tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa ttgatgactc
     2581 ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat cgtcgtaagc
     2641 tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct gagatcgccc
     2701 aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa gaagtcggtg
     2761 gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg aaacaaattg
     2821 caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat gaagaggaca
     2881 atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca aaaccacact
     2941 gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa gtagccggta
     3001 gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc tacaacttca
     3061 tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct tatatcgtca
     3121 acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat gttttccaat
     3181 tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact aactattata
     3241 atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta agccctgctt
     3301 ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt cttcaccaat
     3361 tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa gaattggaaa
     3421 aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat cgtgtgatgg
     3481 ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta gaagtatgga
     3541 tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa gatttccaag
     3601 cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat actcatactc
     3661 tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa aactaccaaa
     3721 atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg aatctgaaag
     3781 ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga aaaattttgt
     3841 gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac tggcttttga
     3901 acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag agaaaaaaat
     3961 ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt cagctgattg
     4021 aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac aaggacaaat
     4081 tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag gtggcaaggg
     4141 aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
     4201 aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg aaggagttgc
     4261 ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
     4321 cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct tttgtgattt
     4381 gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata aattgcgctt
     4441 catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa cgcaaaccgc
     4501 cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga ccttcgattc
     4561 cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg tcttttgaac
     4621 ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga cgtccttgga
     4681 agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct agcatcactg
     4741 catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc cctccgtctg
     4801 caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata gctgtgagct
     4861 gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt ccgataccaa
     4921 cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc gtcgcaacgt
     4981 ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt tttcggatca
     5041 cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct gcttctaata
     5101 aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct acaagcaatc
     5161 gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg tctttgatcg
     5221 tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg attttatgtt
     5281 tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc actaagttgt
     5341 cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc atgtcgcggt
     5401 ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc acaccactga
     5461 tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc gttaagaaga
     5521 acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca tccgcttgct
     5581 ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc atagcaatgg
     5641 ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata ttcaacttaa
     5701 tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga ctttctgctg
     5761 gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt tcccagttgg
     5821 acatttttag taccactcct ctaatttagt ttatctatta aaataattga ttttttcatt
     5881 taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa tataaagcgt
     5941 ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa gtaataagaa
     6001 cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta ccgcagatgc
     6061 aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt tcgagtagta
     6121 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt aataccgaga
     6181 aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt ttgtcacagt
     6241 cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc cctagatatc
     6301 tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa cttccaataa
     6361 tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg ataatgataa
     6421 ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc gcagaaaaca
     6481 gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc attcctggtt
     6541 tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac atcatataaa
     6601 agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa gctggcgttg
     6661 ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca agcaacagca
     6721 acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct gctggcttat
     6781 tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct gttcttccgc
     6841 ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact ttttcttctt
     6901 cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt tgatttcttt
     6961 tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat tgttctggtt
     7021 ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac aaaagacatt
     7081 ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga ataacattac
     7141 gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag cttcttttgc
     7201 tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg cacggataaa
     7261 tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga ttcctttttt
     7321 gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta agcctaacaa
     7381 atcataagca gcacggatca actgatccaa acctgattct tcgattccta aagcttctaa
     7441 aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac gagcactgac
     7501 cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta cataatgatt
     7561 cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa ttggttttgt
     7621 cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt cgatcgttcg
     7681 ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta gttctgccac
     7741 tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt tgttgataga
     7801 ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta gtggatcaac
     7861 acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt gacaaatagc
     7921 gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt tacttgcgcc
     7981 ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt ttttcggatg
     8041 tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc ctacgttggg
     8101 gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga tcgcattaaa
     8161 caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg ccataaattt
     8221 attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc aaaatctcga
     8281 cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc tgcgcccatt
     8341 cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc aacgatatcg
     8401 tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat tgaatattca
     8461 agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg atcttacctt
     8521 tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa cgatcttcgc
     8581 tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc ttttcttttc
     8641 catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt aaattttcag
     8701 ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct aacagcgtac
     8761 gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga tccggtaaag
     8821 aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga ctagctactt
     8881 ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct tcttctaacg
     8941 gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc gcttcttcgt
     9001 caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac gctcgaaaac
     9061 gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg atgatgggtt
     9121 gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact tcttcaaatg
     9181 ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg acaacggtat
     9241 catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca tcgattcctc
     9301 ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc ttggtagact
     9361 tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact aggtgcttct
     9421 gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa gtatttacgt
     9481 acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt cagtaagact
     9541 ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt attcagtaat
     9601 tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat cgaatcactt
     9661 gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat caaaatataa
     9721 tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct tgccatcatt
     9781 gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat actcaaattt
     9841 tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag aacatcatag
     9901 atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt tccttgtgca
     9961 tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc tagattcacc
    10021 gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat ttgtgccatc
    10081 tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt gggtatttct
    10141 ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag acattcggca
    10201 aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct tgttttgctt
    10261 ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct tcttgtttag
    10321 ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga gcagtgacaa
    10381 ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg tcatgatata
    10441 aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt cgtttattca
    10501 gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt atgctaggaa
    10561 accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct acttcaaatg
    10621 ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc gtaatagctg
    10681 taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg aattgattca
    10741 tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat tcttctggta
    10801 tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct ccctacttta
    10861 acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt gaaaagtaag
    10921 aaaagccgat agactttttc tttctagtat gactcataaa atatacacag aagagagaac
    10981 tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt acgtattacg
    11041 ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg atacttaggt
    11101 ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt tggattttct
    11161 gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg atgttttctg
    11221 gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct tttttgatgt
    11281 agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc aattcttcat
    11341 tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt aaaaagtcgc
    11401 ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag tcttggactt
    11461 cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca actgcttctt
    11521 ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg tgacccattt
    11581 ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca gcacgtgaag
    11641 tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc atgacaccga
    11701 tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc aaagctgcat
    11761 tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta ccatttgttt
    11821 gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg agctggtgtg
    11881 gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact ttttctaatc
    11941 cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta gataaacctt
    12001 gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga tgacgtggtt
    12061 tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg cctacccctt
    12121 caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt tggtgagtcg
    12181 tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca ctatctggtg
    12241 tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta acagaatagt
    12301 cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg atagcaaagc
    12361 caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc ggacctgatg
    12421 aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct gtaataacaa
    12481 ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa acaccatctt
    12541 cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct ttttcgatcg
    12601 tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat gctcggacag
    12661 ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg atattcttac
    12721 ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct ggtccgccta
    12781 cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt aataatgttt
    12841 ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct gcgccaacaa
    12901 cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc cttttatttt
    12961 cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact gtcgccgaca
    13021 acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc cacaggcatt
    13081 cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc taacaacgcg
    13141 atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc gctgaagaat
    13201 aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac ggaaattgaa
    13261 aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg tgctggcaaa
    13321 tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa taattgttca
    13381 tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc tgcttctgct
    13441 aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt ttcccgaatt
    13501 ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc ttcgattaca
    13561 tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa caaatggttt
    13621 aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa cccttcccgc
    13681 agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg gatcatctgc
    13741 gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc tggataatca
    13801 atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga gcggatcaaa
    13861 gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc tgtttttgcc
    13921 cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt caagaatgct
    13981 cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa tagctgcaaa
    14041 atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc ttcccgcgta
    14101 aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg attgctcttg
    14161 ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa tcgtttgttt
    14221 ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa acgaacgata
    14281 ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa ttctaaagta
    14341 atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg cccactccac
    14401 cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg attaaattct
    14461 ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt agatgaagtt
    14521 gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc tttgcgttct
    14581 ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt aaaaaaaacg
    14641 gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa atatgtgagc
    14701 ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga tgtcgtttta
    14761 tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt tgtggaatgc
    14821 ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt aacaacgaag
    14881 aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa ctgtcatttt
    14941 gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc ttatggaaca
    15001 gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa agcggatcaa
    15061 aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact tcatcgtata
    15121 ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg tcaacgtgat
    15181 ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac taaataacga
    15241 taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa tgcagcatgg
    15301 atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc tgtccgtttt
    15361 actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt ttcacggtaa
    15421 ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat gaagacttgt
    15481 gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat gccttgcttt
    15541 tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt gacaagagct
    15601 ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt taaagctgct
    15661 tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc gttgactacc
    15721 tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc tactgtttca
    15781 gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg acccattcct
    15841 aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt ggccaaaccc
    15901 aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata aatcggcatt
    15961 agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc ttaagagcac
    16021 gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt ttgaaagggt
    16081 tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag tataatgaaa
    16141 gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg aaattcatga
    16201 tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta cttgcaaaag
    16261 taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct aagttcaacc
    16321 ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat aacgccatca
    16381 agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg ccattttctg
    16441 catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct cttgaactat
    16501 attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc atgctctttg
    16561 tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat aaaatgatcc
    16621 ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa taaacaatat
    16681 aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa ccggataaga
    16741 cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc ttcacttact
    16801 cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat tggagcgaac
    16861 ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat ccaactccgg
    16921 atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga cttcattgcg
    16981 gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat gaggctgtcc
    17041 cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc ccgtatggaa
    17101 cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta gttttctttc
    17161 cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc ctttcatcat
    17221 ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag ctgaaagaac
    17281 ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag tactcatacg
    17341 tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg ttcttttcat
    17401 atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact ttgatagtat
    17461 aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa aacttatcca
    17521 caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag ggattatcca
    17581 cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt aatccacaat
    17641 caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt ttttatccac
    17701 agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt agtggtgtgg
    17761 aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg tgtggataat
    17821 tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa aatgctaaat
    17881 tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc cctcgatgct
    17941 ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag ctataataca
    18001 tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt tgaggttcct
    18061 agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt tgaagtcggt
    18121 tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga agcagagcaa
    18181 gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct aaaaaaagcc
    18241 atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa ccagatggct
    18301 catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc gctcttcttt
    18361 tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca tcaaatgctg
    18421 caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc taatgatttc
    18481 atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg aaatgttgat
    18541 ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca agaagagttt
    18601 ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac cagtgatcgg
    18661 ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc atgggggctg
    18721 tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg taaaaaagcg
    18781 gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg acaaattgat
    18841 tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc tgcgatgaat
    18901 agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa atcaggaaaa
    18961 ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta ttatcacatc
    19021 caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc tagacaaatc
    19081 gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg agctgaattc
    19141 ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca gctgatggat
    19201 tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact aaattaattt
    19261 gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc aaaagttata
    19321 cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt tttccacaga
    19381 atcaacatgc cctattactt ttattattct ttttaactaa taataaataa acaaacattc
    19441 aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag gaattgcaaa
    19501 ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt gtaaaaatca
    19561 cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca attgaaactt
    19621 ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt tccattgttt
    19681 tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca tttactttag
    19741 aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt atcgtaaatg
    19801 gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac cagatgaaat
    19861 tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc tcacaacatg
    19921 agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct ttattagctg
    19981 tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa gcggctgatc
    20041 attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca ttaacaaatg
    20101 aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa acagaaacga
    20161 tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt ttgattcctt
    20221 caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg atcgaacggg
    20281 cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt cgtccagatg
    20341 cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc ttgagttata
    20401 cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg aaagcagcgt
    20461 tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt cgtccattta
    20521 cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct gtacgtacaa
    20581 attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga aaaaataaaa
    20641 ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt gtttaaatca
    20701 ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg ttttattgcg
    20761 aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg gcattgaaac
    20821 aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag
    20881 ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg
    20941 gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag
    21001 aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg
    21061 tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc
    21121 atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta
    21181 gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat
    21241 caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta
    21301 ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact
    21361 tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc
    21421 agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat
    21481 ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa
    21541 ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca
    21601 gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg
    21661 gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc
    21721 accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca
    21781 cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat
    21841 cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga
    21901 cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag
    21961 acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt
    22021 caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca
    22081 cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg
    22141 aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag
    22201 agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg
    22261 gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg
    22321 ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa
    22381 aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa
    22441 aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg
    22501 gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgttaacg
    22561 cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat
    22621 atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa
    22681 caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt
    22741 caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga
    22801 ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatta
    22861 aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt
    22921 tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc
    22981 attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga acacatgaat
    23041 caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga
    23101 tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta
    23161 tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt
    23221 ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg
    23281 aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc agagctcgtc
    23341 tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc
    23401 tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg
    23461 tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca
    23521 tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg
    23581 caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg
    23641 atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg
    23701 cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag
    23761 ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta
    23821 cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc
    23881 ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc
    23941 aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg
    24001 atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc
    24061 gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga
    24121 ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa
    24181 atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat
    24241 gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt
    24301 gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa
    24361 taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc acagccattc
    24421 agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt
    24481 gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt
    24541 gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca
    24601 gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta
    24661 ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg
    24721 ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat
    24781 tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt
    24841 aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa
    24901 cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt
    24961 tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc
    25021 cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg
    25081 aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc
    25141 gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta tatcgaacat
    25201 cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga agcaagggca
    25261 cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc
    25321 cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc
    25381 gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg
    25441 gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt
    25501 ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa
    25561 cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat
    25621 gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa
    25681 cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg
    25741 ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg
    25801 ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac
    25861 gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc gagtgaaagc
    25921 atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg
    25981 acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat
    26041 ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat
    26101 ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa
    26161 gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct gcgtgaggaa
    26221 gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt gatcacagaa
    26281 aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa
    26341 gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt
    26401 tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta
    26461 tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct
    26521 gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa
    26581 gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa gaatgaccga
    26641 ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt
    26701 cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca gagcttgtga
    26761 gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac
    26821 agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa
    26881 cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa
    26941 cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa
    27001 cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc
    27061 tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt
    27121 taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga
    27181 ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt
    27241 acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa
    27301 accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag
    27361 aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa
    27421 ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca
    27481 acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc
    27541 aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc
    27601 aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt
    27661 caacgatcga catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat
    27721 agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct
    27781 aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa
    27841 cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt
    27901 actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa
    27961 taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg
    28021 taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt
    28081 agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct
    28141 cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt
    28201 tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat
    28261 gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga
    28321 aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc
    28381 aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt
    28441 aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt
    28501 ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt
    28561 taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat
    28621 tggtatttta tctattgata actatgcaga tgccattgat aaattggatg acaaagagat
    28681 ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt
    28741 ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa
    28801 aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca
    28861 gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat
    28921 cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt
    28981 cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa
    29041 tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga
    29101 tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc
    29161 tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa
    29221 cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga
    29281 attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct
    29341 cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca
    29401 atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc
    29461 tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat
    29521 ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc
    29581 agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt
    29641 tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc
    29701 ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc
    29761 tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa
    29821 aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg
    29881 gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat
    29941 catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac
    30001 gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat
    30061 atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt
    30121 aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat
    30181 aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca
    30241 caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa
    30301 aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt
    30361 tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat
    30421 aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt
    30481 aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc
    30541 ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta
    30601 tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc
    30661 ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact
    30721 atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga
    30781 agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg
    30841 caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc
    30901 attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt
    30961 tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa
    31021 gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac
    31081 ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa
    31141 aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag
    31201 caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg
    31261 gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa
    31321 aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa
    31381 ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt
    31441 ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca
    31501 gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg
    31561 ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa
    31621 gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat
    31681 gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa
    31741 aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa
    31801 gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag
    31861 ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac
    31921 aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc
    31981 gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga
    32041 aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca
    32101 cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca
    32161 agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga
    32221 agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg
    32281 gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg
    32341 acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct
    32401 ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg
    32461 ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg
    32521 ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa
    32581 agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt
    32641 ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc
    32701 aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg
    32761 tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt tttaataacc
    32821 attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat aaaaatcatt
    32881 cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta aataatatag
    32941 aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc gatgaaggga
    33001 aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg
    33061 gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg catttgattc
    33121 cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc
    33181 ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt
    33241 tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg gaccaattac
    33301 aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc ggacctgcct
    33361 atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat aaagaaattt
    33421 ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta aaaatgtttg
    33481 attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac ggacaacaaa
    33541 tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat gcaggcaaac
    33601 gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga acttatcctt
    33661 ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt gtagggcctt
    33721 caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac
    33781 catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta ggacgagaat
    33841 acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc
    33901 attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat gtattaagtg
    33961 gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta atctaccact
    34021 atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct
    34081 ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac gcacgtaact
    34141 atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct gtcggacctg
    34201 atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga taaaaacgac
    34261 gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac gataataata
    34321 acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34381 ttccgaa
//
LOCUS       DAYYJC010000020        32178 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000020 DAYYJC010000000
VERSION     DAYYJC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.020"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACHA9N_000939"
     CDS             complement(407..766)
                     /locus_tag="ACHA9N_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9084420.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACHA9N_000940"
     CDS             complement(796..1137)
                     /locus_tag="ACHA9N_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084421.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACHA9N_000941"
     CDS             complement(1134..1808)
                     /locus_tag="ACHA9N_000941"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9084422.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA9N_000942"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACHA9N_000942"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9084423.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACHA9N_000943"
     CDS             complement(4322..5512)
                     /locus_tag="ACHA9N_000943"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9084424.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACHA9N_000944"
     CDS             complement(5533..6060)
                     /locus_tag="ACHA9N_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9084425.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACHA9N_000945"
     CDS             complement(6107..8896)
                     /locus_tag="ACHA9N_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9084426.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACHA9N_000946"
     CDS             complement(9043..9243)
                     /locus_tag="ACHA9N_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9084427.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACHA9N_000947"
     CDS             complement(9696..10016)
                     /locus_tag="ACHA9N_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084428.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACHA9N_000948"
     CDS             complement(10519..11637)
                     /locus_tag="ACHA9N_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084429.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACHA9N_000949"
     CDS             11870..11992
                     /locus_tag="ACHA9N_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084430.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACHA9N_000950"
     CDS             12108..13415
                     /locus_tag="ACHA9N_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9084431.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACHA9N_000951"
     CDS             13536..14735
                     /locus_tag="ACHA9N_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9084432.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACHA9N_000952"
     CDS             complement(14762..16030)
                     /locus_tag="ACHA9N_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9084433.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACHA9N_000953"
     CDS             complement(16200..16841)
                     /locus_tag="ACHA9N_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9084434.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACHA9N_000954"
     CDS             complement(17250..17753)
                     /locus_tag="ACHA9N_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9084435.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACHA9N_000955"
     CDS             complement(17876..18640)
                     /locus_tag="ACHA9N_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9084436.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACHA9N_000956"
     CDS             18748..19023
                     /locus_tag="ACHA9N_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9084437.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA9N_000957"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ACHA9N_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9084438.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ACHA9N_000958"
     CDS             complement(20579..20980)
                     /locus_tag="ACHA9N_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9084439.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ACHA9N_000959"
     CDS             complement(20994..21803)
                     /locus_tag="ACHA9N_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9084440.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ACHA9N_000960"
     CDS             complement(21790..22680)
                     /locus_tag="ACHA9N_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9084441.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ACHA9N_000961"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="ACHA9N_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="ACHA9N_000962"
     CDS             complement(23348..24289)
                     /locus_tag="ACHA9N_000962"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9084442.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA9N_000963"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ACHA9N_000963"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9084443.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ACHA9N_000964"
     CDS             complement(25274..26470)
                     /locus_tag="ACHA9N_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9084444.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ACHA9N_000965"
     CDS             complement(26488..27216)
                     /locus_tag="ACHA9N_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084445.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA9N_000966"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ACHA9N_000966"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9084446.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ACHA9N_000967"
     CDS             complement(28628..29086)
                     /locus_tag="ACHA9N_000967"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9084447.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ACHA9N_000968"
     CDS             complement(29083..30546)
                     /locus_tag="ACHA9N_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9084448.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA9N_000969"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ACHA9N_000969"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9084449.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ACHA9N_000970"
     CDS             31602..>32178
                     /locus_tag="ACHA9N_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9084450.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggt aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAYYJC010000021        31888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000021 DAYYJC010000000
VERSION     DAYYJC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.021"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..173)
                     /locus_tag="ACHA9N_000971"
     CDS             complement(<1..173)
                     /locus_tag="ACHA9N_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9084451.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYL"
     gene            complement(175..372)
                     /locus_tag="ACHA9N_000972"
     CDS             complement(175..372)
                     /locus_tag="ACHA9N_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9084452.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(766..2493)
                     /locus_tag="ACHA9N_000973"
     CDS             complement(766..2493)
                     /locus_tag="ACHA9N_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9084453.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2486..3679)
                     /locus_tag="ACHA9N_000974"
     CDS             complement(2486..3679)
                     /locus_tag="ACHA9N_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9084454.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3866..4510)
                     /locus_tag="ACHA9N_000975"
     CDS             complement(3866..4510)
                     /locus_tag="ACHA9N_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084455.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4604..4738)
                     /locus_tag="ACHA9N_000976"
     CDS             complement(4604..4738)
                     /locus_tag="ACHA9N_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9084456.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4740..6872)
                     /gene="feoB"
                     /locus_tag="ACHA9N_000977"
     CDS             complement(4740..6872)
                     /gene="feoB"
                     /locus_tag="ACHA9N_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9084457.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6869..7342)
                     /locus_tag="ACHA9N_000978"
     CDS             complement(6869..7342)
                     /locus_tag="ACHA9N_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9084458.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7743..7967
                     /gene="nrdH"
                     /locus_tag="ACHA9N_000979"
     CDS             7743..7967
                     /gene="nrdH"
                     /locus_tag="ACHA9N_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9084459.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8126..10285
                     /gene="nrdE"
                     /locus_tag="ACHA9N_000980"
     CDS             8126..10285
                     /gene="nrdE"
                     /locus_tag="ACHA9N_000980"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9084460.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10335..11300
                     /gene="nrdF"
                     /locus_tag="ACHA9N_000981"
     CDS             10335..11300
                     /gene="nrdF"
                     /locus_tag="ACHA9N_000981"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9084461.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11389..12528)
                     /locus_tag="ACHA9N_000982"
     CDS             complement(11389..12528)
                     /locus_tag="ACHA9N_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9084462.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12837..13550)
                     /locus_tag="ACHA9N_000983"
     CDS             complement(12837..13550)
                     /locus_tag="ACHA9N_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9084463.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13804..14505)
                     /gene="nagB"
                     /locus_tag="ACHA9N_000984"
     CDS             complement(13804..14505)
                     /gene="nagB"
                     /locus_tag="ACHA9N_000984"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9084464.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14739..16271
                     /locus_tag="ACHA9N_000985"
     CDS             14739..16271
                     /locus_tag="ACHA9N_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9084465.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16462..17664)
                     /locus_tag="ACHA9N_000986"
     CDS             complement(16462..17664)
                     /locus_tag="ACHA9N_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9084466.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17746..18435)
                     /locus_tag="ACHA9N_000987"
     CDS             complement(17746..18435)
                     /locus_tag="ACHA9N_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9084467.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18541..18909)
                     /gene="rplL"
                     /locus_tag="ACHA9N_000988"
     CDS             complement(18541..18909)
                     /gene="rplL"
                     /locus_tag="ACHA9N_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9084468.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18962..19465)
                     /gene="rplJ"
                     /locus_tag="ACHA9N_000989"
     CDS             complement(18962..19465)
                     /gene="rplJ"
                     /locus_tag="ACHA9N_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9084469.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19669..19796)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19875..20564)
                     /gene="rplA"
                     /locus_tag="ACHA9N_000990"
     CDS             complement(19875..20564)
                     /gene="rplA"
                     /locus_tag="ACHA9N_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9084470.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20668..21090)
                     /gene="rplK"
                     /locus_tag="ACHA9N_000991"
     CDS             complement(20668..21090)
                     /gene="rplK"
                     /locus_tag="ACHA9N_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9084471.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21433..22101
                     /gene="sdaAB"
                     /locus_tag="ACHA9N_000992"
     CDS             21433..22101
                     /gene="sdaAB"
                     /locus_tag="ACHA9N_000992"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9084472.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22114..22986
                     /gene="sdaAA"
                     /locus_tag="ACHA9N_000993"
     CDS             22114..22986
                     /gene="sdaAA"
                     /locus_tag="ACHA9N_000993"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9084473.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23077..24177)
                     /locus_tag="ACHA9N_000994"
                     /pseudo
     CDS             complement(23077..24177)
                     /locus_tag="ACHA9N_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24269..25156)
                     /locus_tag="ACHA9N_000995"
     CDS             complement(24269..25156)
                     /locus_tag="ACHA9N_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9084474.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25233..25778)
                     /gene="nusG"
                     /locus_tag="ACHA9N_000996"
     CDS             complement(25233..25778)
                     /gene="nusG"
                     /locus_tag="ACHA9N_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9084475.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25879..26049)
                     /gene="secE"
                     /locus_tag="ACHA9N_000997"
     CDS             complement(25879..26049)
                     /gene="secE"
                     /locus_tag="ACHA9N_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9084476.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26073..26225)
                     /gene="rpmG"
                     /locus_tag="ACHA9N_000998"
     CDS             complement(26073..26225)
                     /gene="rpmG"
                     /locus_tag="ACHA9N_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9084477.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26387..27031
                     /gene="cbpA"
                     /locus_tag="ACHA9N_000999"
     CDS             26387..27031
                     /gene="cbpA"
                     /locus_tag="ACHA9N_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9084478.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27087..27590)
                     /locus_tag="ACHA9N_001000"
     CDS             complement(27087..27590)
                     /locus_tag="ACHA9N_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9084479.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27603..27649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28187..29152
                     /locus_tag="ACHA9N_001001"
     CDS             28187..29152
                     /locus_tag="ACHA9N_001001"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9084480.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29251..30423)
                     /locus_tag="ACHA9N_001002"
     CDS             complement(29251..30423)
                     /locus_tag="ACHA9N_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084481.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30410..30535)
                     /locus_tag="ACHA9N_001003"
                     /pseudo
     CDS             complement(30410..30535)
                     /locus_tag="ACHA9N_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30643..31722
                     /locus_tag="ACHA9N_001004"
     CDS             30643..31722
                     /locus_tag="ACHA9N_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9084482.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9728 a         6588 c         5729 g         9843 t
ORIGIN      
        1 agataattca ccaatctatt tattaaagga ccgggatcta attcatattt ttgtttttct
       61 aattttaaat ataccttttg taaaacctca agaatatcag aatatttagg atctttactt
      121 atagatatat cattatataa ttcatgaaca atttgcttag catttttttt cattttagca
      181 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
      241 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      301 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      361 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      421 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      481 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
      541 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      601 cgcacccata aattcataat agtttgtcgg cgtctttgga cgtttcgaag atgagctacg
      661 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      721 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      781 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      841 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      901 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      961 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
     1021 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
     1081 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
     1141 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
     1201 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
     1261 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
     1321 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
     1381 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
     1441 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1501 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1561 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1621 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1681 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1741 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1801 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1861 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1921 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1981 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     2041 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     2101 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     2161 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     2221 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     2281 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     2341 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     2401 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     2461 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2521 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2581 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2641 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2701 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2761 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2821 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2881 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2941 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     3001 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     3061 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     3121 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     3181 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     3241 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     3301 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     3361 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     3421 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3481 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3541 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3601 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3661 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3721 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3781 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3841 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3901 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3961 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     4021 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     4081 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     4141 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     4201 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     4261 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     4321 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     4381 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     4441 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4501 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4561 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4621 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4681 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4741 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4801 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4861 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4921 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4981 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     5041 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     5101 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     5161 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     5221 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     5281 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     5341 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     5401 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     5461 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5521 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5581 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5641 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5701 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5761 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5821 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5881 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5941 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     6001 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     6061 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     6121 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     6181 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     6241 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     6301 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     6361 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     6421 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6481 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6541 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6601 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6661 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6721 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6781 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6841 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6901 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6961 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     7021 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     7081 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     7141 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     7201 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     7261 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     7321 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     7381 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     7441 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7501 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7561 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7621 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7681 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7741 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7801 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7861 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7921 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7981 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     8041 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     8101 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     8161 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     8221 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttctatc
     8281 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     8341 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     8401 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     8461 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8521 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8581 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8641 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8701 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8761 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8821 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8881 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8941 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     9001 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     9061 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     9121 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     9181 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     9241 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     9301 tgtgattaac ggcaaacagg aatatgaagt attaggaacg gatatctcat gtaacttagg
     9361 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     9421 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9481 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9541 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9601 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9661 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9721 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9781 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9841 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9901 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9961 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
    10021 tatggacatg cgtaaagtaa tcgatgttta tgcaatggca caacaacacg tcgatcaagg
    10081 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
    10141 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
    10201 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
    10261 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
    10321 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
    10381 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
    10441 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10501 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10561 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10621 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10681 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10741 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10801 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10861 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10921 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10981 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    11041 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    11101 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    11161 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    11221 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    11281 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    11341 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    11401 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    11461 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11521 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11581 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11641 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11701 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11761 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11821 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11881 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11941 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    12001 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    12061 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    12121 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    12181 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    12241 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    12301 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    12361 ataatagata aaaccagcaa caagcaatgg taaaaaaact gccgaaagca ttttcaatat
    12421 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12481 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12541 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12601 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12661 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12721 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12781 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12841 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12901 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12961 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    13021 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    13081 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    13141 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    13201 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    13261 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    13321 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    13381 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    13441 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13501 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13561 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13621 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13681 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13741 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13801 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13861 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13921 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13981 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    14041 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    14101 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    14161 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    14221 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    14281 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    14341 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    14401 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    14461 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14521 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14581 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14641 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14701 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14761 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14821 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14881 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14941 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    15001 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    15061 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    15121 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    15181 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    15241 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    15301 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    15361 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    15421 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15481 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15541 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15601 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15661 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15721 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15781 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15841 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15901 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15961 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    16021 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    16081 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    16141 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    16201 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    16261 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    16321 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    16381 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    16441 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16501 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16561 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16621 gtacataaac ataaatacag tattatttcg gttgcttgct gcttcaccta gcccttgtac
    16681 tcgtgtttga ttggcgactt gaccaaattg attcccgacg tctaataaaa taatagctaa
    16741 aagcaaaatg gccaaatggc ttccgccaac caataaagca ataaagctta ttgtttgcat
    16801 aacaagtccc atatacacga tttttctttc agaataagaa tctgccagtc gtcccacgat
    16861 aggtgcagca actgctccag acaaaccaaa aatcgctaat atccctgctt cgaatgttcc
    16921 ccaattgtag gcgggactgc tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt
    16981 tccaaacatg aaaaatccac tgactgccgc ttctcttaat agacgttgag aagcaactaa
    17041 atgcggcaaa cttttaagtg aatcaaggta tgaaacagct gtactacttt gctggacaga
    17101 acgcggcatt ttccagtaaa gcagaagtgt aagaattccg actgcacata aagcaaataa
    17161 atagatcatc cgccaagaaa aaagactggc aacaatacca gataccgtcc gagaaagcaa
    17221 aatccccacg agtaaaccgc taagcaatgt acccatgacc ttccctcttg cttttggtcc
    17281 cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg atcgaaagta atcctattaa
    17341 aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa aaagcggcca acagagagac
    17401 ggcagataac gctgccattc gaatgatcaa ttttggacga tccaccacgt cccctaaagg
    17461 aactaaaaac agtaacccta acgcatagcc taattgggtc aacatcgtca atatcccgac
    17521 tgcacttgtg ctaacagata aactagctgc tacttttgtt ccaatcggct ggatgtaata
    17581 catattggca acaacaacgc cacaagtgat tgataatagt aaaataagcg aagttgttaa
    17641 aggttttttt tcattactct tcattgattt cccccatttc ctatcttagt ttacaactta
    17701 tggaaaagat tggaaataga aaaatacaac tattcgccac acttattatt tgtgcttttg
    17761 ttttccttgt tttttcttgc gatctttata aatgaagaca actaacccta ctagcacagt
    17821 aacacttgca accaacgtga ctgcagtttt gttatatccg tgaaacagag cttctccacc
    17881 acacgcatac aacacagacg aagggatgac tccaatcacg atcgacaata tgatttgctg
    17941 tagaggcaat ttcatcgcat ctgcagcaaa attgaccata gaagatggaa taaccggtat
    18001 catatagcct aacattacac ctatttttgg atgtttcatt tgtcggattg cttgcaccca
    18061 atgattcgtt tcggttttct tatctaaaaa ttttaagcga tgcataagga aaattgcagt
    18121 gatgttacca agagcattac ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct
    18181 caaaccagca atgactccaa ctactgaagt aggaatacct ggaacagcac acatcaccgc
    18241 tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag
    18301 ctctgcctta ctcgctctcg gatttaaaaa taattggatg tctgtctgat attctaaata
    18361 caaccgatag cccactacca gtatcagaat gattcccaac gagatcaata tcaatcggat
    18421 tattttcttt ttcatatttc ctcctgaaaa aaatctatat tcattaaata atagtttaac
    18481 ataaaaacaa aaaagcaccg aaatccaaca taggatctca gtgcttttaa atagtaaaaa
    18541 ttatttaaca gttacagatg cgccaacttc ttcaagttta gctttcaatt cttcagcttc
    18601 ttctttagat acgccttctt taactggtgc aggagcgcca tcaactacag ctttagcttc
    18661 tttcaagcct aaaccagttg cttcacgaac tgctttgata actttaactt tttggtctcc
    18721 agctgcagtt aattctacag tgaattctgt ttgttcttca gcagcagcag cgccaccagc
    18781 agcagctaca gctacaggag cagcagcaga tacgccaaat tcttcttcaa tagctttaac
    18841 taggtcgtta agttctagga tagttgcttc ttttaactca gcaacgatgt tttcaatgtt
    18901 caatgccatt ataatttcct ccgtttaatt gttctttttt atttaatgct ttatgcggca
    18961 attaagctgc atctccatct tctttgtctg ccactgcttt gacagcgtaa gccacgttgc
    19021 ggactggcgc ttgtagtaca gatagcaaca tagatagaag tccttcgcga tttggcaatt
    19081 ttgccaatgc tgtgatttct tccaatgaag agactttacc ttcgatgata ccgcctttga
    19141 tttctaatgc ttttgcttct ttagcaaatt cgtccatgat tttcgctggt gcgactacat
    19201 cttcgttact gaatgcaaca gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc
    19261 cagctttttt agctgcacgt gaaagaatag aatttttgat aactttcatt tctacgttag
    19321 catcacgaag ttgtttacgt aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa
    19381 cgactactac tgatgcagct gctgtaaatt tttcgcttac ttcatcaacg attgctgctt
    19441 tttttgcgat aattgcttca ctcatttttg agtttcacct cctgaatttt gatggaagag
    19501 ttttttgcac tgaaaaggca agtcactttt atagttactt tgctcaacca gtattattca
    19561 tcacctattc acttcgttca tagtgattct actcgctttg ctcgaaagga aactcctttc
    19621 gctttgctca accagtattg ttcatcatct cttcacttcg ttcatagtga ttcacaataa
    19681 aaaactctat gccaccgcag acatagaggg actattgaat atattcaata aacgtcctcg
    19741 gtaggaaatt aaggcttgat gccacctact gtcttcggta caagttaatt attaacctca
    19801 actagcttag catgctaaac tagttgagtc aagaattatt tttcagttgg atcaatcaac
    19861 attctaatat ttaattagaa agaagcttgg tcaacatgga taccaggtcc aaatgttgtt
    19921 gtgatcgtta catttttcat gtattgacct ttagctgctg atggtttagc ttttagcaat
    19981 acatcatgga ttgctgcaaa gttttcaact aatttttcat cgtcaaatga aacttttccg
    20041 attggtacat ggatatttcc agctttgtcc acacggtaag ttactttacc agctttcact
    20101 tcgtttactg cttttgttac gtccattgta actgtaccag ttttagggtt tggcattaag
    20161 ccttttggtc ctaatactcg tcctagacga cctacagtag ccatcatatc aggtgttgca
    20221 acaactacat caaagtcaaa ccatccgttt tggatttttt gaaccatgtc gtcgtcacct
    20281 acgaaatctg caccagctgc ttcagcttct tttgctttgt ctcctttagc aaaaactaaa
    20341 acagtttgtg ttttaccagt tccgtttggt aatacaacag caccacggat ttgttggtct
    20401 gcttttttag ggtctacgtt caaacggtat gcaacttcaa ctgttgcatc aaattttgca
    20461 atgtttgttt cttttgctaa agctactgct tcggcaacag gataaacttt acttgaatca
    20521 acttttttta atgcatcttg catttttttg ctctttttag ccattttggt tcctccttga
    20581 ttgtggttat aacggttgta cctcccacgt gttctatatc tttcaatata gagccaactg
    20641 agaagctgct gtcttagggt gtatagatta ttctacagtg atccccatgc ttcgtgcagt
    20701 tccttcaacc atgcgcattg ctgcttcaac gttagctgcg tttaggtctt ccatttttaa
    20761 ttcagcgatt tctcttactt gatcgctaga aactttcgca actttatttt tgtttggttc
    20821 gcctgaacct ttttcgattt tagctgcttt tttcaataga acagcagctg gcggtgtttt
    20881 tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata acaaccggaa tgatcaaacc
    20941 tgcttgatca gctgtacgag cgttgaattc ttttgtgaat cccatgatat tgatacccgc
    21001 ttgacctagt gcaggaccta ctggtggagc tggagttgct ttacctgcag gaatttgcaa
    21061 tttaacgatt ttttctactt tctttgccac gagacatacc tccttaagtc cgtgatgtgg
    21121 ttaattggag atgcatattt ctcctcccac gttttatctg atctgaaaaa atccagacaa
    21181 ataatgtgca aaacaatgca cactggtaca gtatagcaaa tttatttgtt ttttcaagat
    21241 ttttttctgc tatttttaca ttttgcttga ataaacgcta aatttattct aaaaaaacaa
    21301 caatttttcg ttctcatttc ttcttttatg ttacacttgc gctagcatgg ttcaacagat
    21361 gaaagcaaac aataacacgg ttttttagac ccttgtacaa agattcctac ataacaaaat
    21421 gaggtggaga aaatgaaaga tttaaggtat agaagtgtct ttgatattat tggacctgtg
    21481 atgattggtc caagtagctc tcacactgcc ggagctgcta gaatcgggaa aatcatacgt
    21541 agtatatttg gcgaacagcc tgattctgta gatatttatc tatatgaatc atttgcaaaa
    21601 acctatcggg ggcacggaac agatattgca ttagttggcg gcctgttagg gatggaaccc
    21661 gatgatgaac gtttagctga ttctttgaaa ttggcatatg aagccggtat ggaagtcttg
    21721 tttgttccta agagtgaaaa agccgaacat ccaaactctg taaagatgct tctaaaaaaa
    21781 ggaacacgaa agctttcagt cactggcatt tcgatcggcg gtggcaatat ccagatttct
    21841 gaattgaatg gattcaaaat ttctttaagc atgggtactc ctaccttgat cgttgtccac
    21901 caagatgttc caggtatgat tgctaaagtg actagtatac tttctgagac caatacgaat
    21961 atcggcacta tgacagtgac acgggaatca aaaggtgaaa atgccattat gataattgaa
    22021 gtagatgatc ctcaagtaga agaaacggta aaaagactca aacagctgcc aaatatcgac
    22081 agcgtaaatt atttcgatta aggagctaaa aaaatgtttt tatcgattga agaattagta
    22141 gatcaagcaa aagcttatcc aagtgtagca gaattgatga ttgcagttga aatggaaatg
    22201 agtggccgtt cccgtagtca catcatcgag atcatggaaa aaaacctctc agtaatggaa
    22261 caatcaatca acgagggaaa agaaggtgtt acctccgtca caggaattac cggtggtgat
    22321 gcaaaaaaat tagatgatta tttaaatgag gcagactttt taagtggaga aacaatctta
    22381 aaagctgtac gcaatgctgt agctgttaat gaagtcaatg caaaaatggg gttgatttgt
    22441 gctacaccga ctgccggaag cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat
    22501 cggcttgatt tgacacatga acagcaattg gaattcttat ttaccgctgg cgctttcggg
    22561 ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag ggggatgtca agcagaagtt
    22621 ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg caaaaaatgg gactcctcat
    22681 caagcagcac aagcagtagc cattacattg aaaaatatga tgggactgat ttgcgatccc
    22741 gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct
    22801 tttatctctg ctgatatggc attagcgggt atcgaaagtg tcattccgcc ggatgaagtc
    22861 gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa
    22921 ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg aagagatttt aaataagagg
    22981 gcgtgataaa gctccttaat ttcgagtatt aagagattag ttatcttcat aacaaaaaac
    23041 gaagagagga ctaattgccc ttctcttcgt tttttattaa tccatcaatt tcgtattttt
    23101 tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag
    23161 gtgttcaaaa acagatgtta ctccttctgc cccacctaga ttcaacccat aaatcactgg
    23221 tcggccaatc gcaactaagt cagcaccact tgccagagct ttgaagacat gctctcctcg
    23281 acggacacca gagtcgaaaa tcactggtac acgtttgttg actacttcgg caatttttgg
    23341 caatacttca aaggacgcag gacctccatc tagttgacgt ccaccatgat tggataccca
    23401 gataccatct gcccctgcag agatagcgat tactgcatct tctggcgatt gtattccttt
    23461 tacgataact ggaagatggg tgatttcttt gattgttttg atgtcatctg gtgtcaagcc
    23521 ttgttttgca gctgcataaa tttctgcgat tccttttcct tcgccattac cacttgcact
    23581 ttgttcgcta tacgcagcta agttaggcat tggtaatgga aattggaatt gattgatgac
    23641 gtcttcttca cggtaaccac cgagcgttga atctgccgtt aaaatgatag ctttagcccc
    23701 tgcttctact gctttgttca aaatgaactc attgaatctg tcgtctttac tcatatataa
    23761 ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg tctttgattg tcgtatttgc
    23821 ataagtgctg atggaaaaga ttgatccagc agcagctact cctttagctg tatcagcttc
    23881 ccctttttca tgtgccaatc cttgggccgc tgatggcgct tggataatcg gtgtatctaa
    23941 ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg attccgcgta atatccgtgg
    24001 catgattttc tttgtattaa aagaagtcgt attttctttc atcgtccact catcttcaga
    24061 accaccgcga atatagccaa aagctcctgc ttccatgcgc tcttttacac gaccttctag
    24121 ttaggcaatg tttacaatat cgattggatg ttcttcatta cttgtatgat aagtcatagt
    24181 agatccttct ttcatcttct tgattacata taaatcatac taaacttaca aaaagtaagc
    24241 aaacatttac tctcagatac ttttatcgct attctttcca aataaatcga cttacaagct
    24301 gatctacttc ttggttttca tgaagcatac tatgagaggc attttcacct gtcactactt
    24361 tttcctctat ttcatttccg cgttgcttca acaacgcata gaccgctaat gcactgttta
    24421 acggtactgt tccatcactc aagtctgttt catctagttg accagcaatc aagaaaaatc
    24481 gagtggttat aggaatatta ccgatcattt gctggtaatc ttgatagcgg ttagaaacga
    24541 cagcaggccc ctttgctaac tcatctgtca gagactgcgc actatcatcg acaaaatcat
    24601 taaaaggcgc gccaattgct gttaatgtct gaacttgtgg caatgacgta tcctgtccat
    24661 acgttcctaa atagcgtagt gcactaactc ctcccatgga atgccccact atgttgactt
    24721 tatctatgcc gtagttttct ttcagataca aaagacaagt tttgatccat tctgcttgat
    24781 tccactcgtt atttttatta tcttcaaaca atacttggac catcggattg tctgcttttc
    24841 cagacaaatc tcctgttgct tgtatttgtc catctgttcc tactgttaaa accaattctt
    24901 ttactgcttg ctgatttttt tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc
    24961 cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt ttctatcact ttactggatt
    25021 ctgccgtttt ttctttatca gaaacttcag tagtatttat cttcgtcgtc tggcaaccac
    25081 ttaacgcaaa caaactgata aggactaaag ctgctgcagt tgcacgtttg cataaatttt
    25141 tgattggttc tgtcatacac ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc
    25201 caatcatatg atcgacattt caagtctctt tattaatcga ttttatctac ttgatcgaaa
    25261 tcaagttctg tactggtttc acgtccaaac atatcgatgt tgactttcaa tttttctttt
    25321 tctccgtcaa cttctgttac ttgtccttct aatccagcaa atgcgccttc gatgattttc
    25381 acgacttcgc caacttctac ttctagatca ttttgtcgca ggctcatacc gatagaacgc
    25441 aagatatgat tgacttcttc tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa
    25501 ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat accatgaagc gtctgtcatg
    25561 atcatttcaa caagtacgta tcctgggaat gttttatgaa cgatttcttt ttctttccca
    25621 tttttgactt cagtttcttt ttcttcagga acaacgacac ggaaaataaa gtcggccatt
    25681 ttcatacttt gcgcacgaga ttcgatattt gcttttactt tgttttcata tcctgaatat
    25741 gtatgcaaga cataccagtt tttttcaaaa gattccattg aaggctcctc atttcattgt
    25801 aaaataaaaa aacctcagta cacccgaagt ttttctttcg cttcattata gcactaaaag
    25861 tacgttgtgg ctagcgagtt attttaaaat ccagccaaat aagctttgga tggctgtatc
    25921 cattacgtaa aacatcaatc cgaaaataat agaagtttcg atgactacta acgtatcttt
    25981 acggagttgt tttctgcttg gccaagttac ttgtttgatt tcatctttta cgctttttag
    26041 aaatttcatg agacaacctc ccttgctact gtttatttcg tttctttatg taatgtatat
    26101 tgattacaat atttacaaaa cttattgatt tccaaacgtt cgccgctttt tccttcactg
    26161 actgattttg tgtagttgcg agacccacaa acggaacaag cgagtgctgc ttttttcttt
    26221 gccatttact tctgccccct tgctgagaat cagacttata cttcataaca gtatcacgta
    26281 actttttact tgtcaatttg tgaaataaac tatctattgt tttggataaa attatgatat
    26341 gattaatttg aaattaagct aaaatgagac aattggaggt atagcgatgc tattaaaaac
    26401 acttgtatat aagaaaaaag atctgacaac cgttacagag actagtacat tagaagaagc
    26461 attaaaaatt ttagaaactt ccggataccg ttgtgtgccg atcttagatt cttcaggtaa
    26521 tattttccgt ggaaacattt acaaaatgca tatctatcgt cataaagcaa atggcggtga
    26581 tatgacgctt cctgtcactc atcttttgaa aaacgctaca aagttcattt acctggatac
    26641 ttctttcttc aaagtattct ttacgatcaa agagcttcct tatattgctg tccttgacag
    26701 tgaaaatcat ttttatggta ttttgacaca tagtacgttg cttaatatgc tctctcaatc
    26761 ttggagcgta gaacaaggaa gctatgtact gacaattgct tccacaggaa agcaaggaga
    26821 tttggctgct attgcaaaaa tcatttcaaa acacagcagc attgccagct gtattactct
    26881 tgatatcgaa aaagacgaat acattcgacg tacattgatc acgctaccgg ctaacactga
    26941 agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa aagaattata aagtggtaga
    27001 aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc gaaaaaagaa aacagctaat
    27061 ctgctttctt ttttcgcttt ttataattag acacgctttt caaaatcaag cgcgcgatct
    27121 aaataataca tcaatggtgc tgaaacagcc atgacgataa actcacttaa tgcaagcgtg
    27181 ccataggttg tccaaaatgc ttggccacct gttggaacta acatataagc aatcagtccc
    27241 atgctgatgg taaagaagat cgtgttcaat actaaacgca tctttacatt tttcactttg
    27301 ttttgcaata aagctgtcag gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca
    27361 tccatgatac caaaaccaaa gaaggcatta tagacgatga ccccgcctaa aactccccaa
    27421 agcaatttac ggttaaagac aactaaatga ttcaaacttt ctgaaatacg aaactggatc
    27481 ggccctgagg atacaggcgc aactaaaata gttaacgcta gataaagagc catgatgatg
    27541 ccatttacaa cgattacgga taaccgttgt ctgcttttcg ttttttgcat tttctctcct
    27601 cttttctcct agttttttta acgcgggatg gttgatgaac cgcggttatg tgaaacaaac
    27661 aataattata acagactttt catttatttc aatccgtctt atctgctttt aaaaaagcaa
    27721 aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa
    27781 ggggtcataa gaattcccag taaagcagac gaagtaggat ctgcaagaac agaattgttg
    27841 tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc aacctcttac tagagaacgg
    27901 tctagcaagc agctgaagat ctaagtggac atgccgaacc ttggaacttc tccttacgta
    27961 agtaatggat catgcgcttg ctaggctttt ttgtgttttt cggtcttcgg acctttttca
    28021 ttttcctaga cttctgctat aataaccaag aattaatttt gtaacagatt tttttgaaaa
    28081 aacaagtttc tttcattcaa aaaatagaaa aagcttgcta attattggtt aaccagcaaa
    28141 caggagtatt aaaatatctt gttttattga atagcaggag gaaatcatga aattccatta
    28201 tcatatattg atcaatccat cggcaggcag cggaaatggc tataaagtag cagaaagaat
    28261 tttaccggta ttaaaaaata agcatattga ctatacactt cattatagtg aatacaaagg
    28321 acacgatgct gagattgctg aaactttggc aaaagaaaca ttgattcctt ggatagaaga
    28381 agaacagaaa acaatcgata ctttccccct tttgatcatc gtaggaggtg acggcacctt
    28441 acatcaggta cttgatactt tctatcagat ggaagtagaa ttcccagttg cttatattcc
    28501 agcaggttct ggagatgatt ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg
    28561 acttcagctg attttagcag ctcaatcacc agaaaaagtc cacataatgg cttatgaaga
    28621 aaaaataagt gaaaaaaaag gaatagctgt taataatttc ggtataggat tagatgcagc
    28681 tatcgtccat gcgacaaatc attcttccac aaaaaagcga ttgaacaaat acaacttagg
    28741 atcattttcg tatttattct ctattctccg tgctttattc actcaaaaag gatttccaat
    28801 tctggtagat gcaggaggaa aacaattaag tttttcaaac gcatttttat gtacagcaac
    28861 gaagcatcct ttttttggcg gaggaatcgc gattgttcca actgctgatg cgtctaaacc
    28921 agtcattgat tttgtcgtgg tcgaacgaat caatttcttt aaaatcttgt ggctcattct
    28981 tttacttata cagaaaaaac aattgaaatc gaaatatttc catcactaca caacaaacag
    29041 attacgtatc gtttcaacaa caccgcaaca tggtcaagaa gatggagaag aaatggaaaa
    29101 acgacctttt gatatcacaa taaccacaac taaacagttg ttttggtgtt aataaaagag
    29161 gttttgtcag aagctacatc tggaacaccg tttattcgga aaaaaggact gtgacacgac
    29221 ttttgtcaca gtcctttttt tactatgcag ttaattcttt gcccaattgg gttgccatcg
    29281 atagtttctt tctttaccca aaataaattg gatcattgaa aacaccgcag caaaagcaac
    29341 taagacacaa ccattgaacc aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc
    29401 actgatcaag ttattatttt gaacaaacca atatgttggt gtgaatgccg ctattttgct
    29461 aaccatttct ggcaagtatt ctgctggtac aaacacccca ccgataaagc aactgcctgt
    29521 gataaaaata tctttgatcc caccgctggt ttctgagttt tttaccaatg aagtgataca
    29581 agccgaaaag ctaatgatcg gcaataaaaa caagaaagta tttaaaatat gataccatac
    29641 cgttatatcc cacgaggtgt ggatcactaa cagaaccatt agaagtaaga agccactgaa
    29701 caacagcagc gagtaactaa tcaaactaaa ggttactttg cgattcaagt ttcgtttggt
    29761 aatgggcgca ctctggtttc gtttactgat ctctttgcga ttaaatgcta gattgatgac
    29821 gctgaaaccg ctaaaaatgg atagaaacaa cccataagac aagaaattaa aggttgtgcc
    29881 cgtcaacagc tttttcatcc gctgactata tcccgcactt agctcaactt ctccagtaac
    29941 cgagagtgcc atttgtgtct gttccatagc tgcatcgatt gttttatctt cttcttgaaa
    30001 cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta acaactgttt tagaaaaggc
    30061 atctgggcga ctctgtatct tgatcgtcgg catctttcct acttcgactt gttcggtaaa
    30121 gtctgcaggc agatgtaaga taatgtttac acgattaaaa taaagagcat catcgatttc
    30181 tttttgtgag gttccatcca atgtaacgac tgtttccttg tcattcaaat atttacgaag
    30241 gccatcgact acaggcgact cttcttcact aatcaaggcg attttcgcat cttgcagaga
    30301 ggtattttca acatcaatat tttgcgcaaa aaagaatgtg gcaacaacag tgataattac
    30361 acctatcagt aaattcgatt tatttgcttt aagcaatgtc caaatcgttt tataaagcgt
    30421 cataacgcac ccctctttcc atccaagcat ttcctaacgc aaaaaggaaa ataaatccga
    30481 tcaaccatag aagattggta taaaaactag ccaaatcagt gtagaaataa agtttaggcg
    30541 gattttataa tgaacttagc catttaaaat aaaaaaaacg ccacgtgaat tccatgttat
    30601 attgaagtta ccacaactct caatagacaa ggatgaattc acatgacgca aacccatgat
    30661 aacacagttt cacgcaaggg aaaacatctt tcctactcag aacgttgcca aatcgctatc
    30721 ctgaaaaagg aaggctattc caaccgtcag gttgccaaag tgctgggacg tgttccgcaa
    30781 accataaaca atgagattga tcgcggaacc atcacccagt taaaacgcca aacgcaaaat
    30841 ggaaaagtgt atgacaccta ctattctgtt tatgacgctg atgcaggaca ggccttctat
    30901 gaaaagcaac gactgaactg tggcagacgc cccaaatggg cgaacacaaa tgccttcatt
    30961 gattgggcag atgacaaaat gttaaatgaa aaatggtcgc cggacgtcgt ggtcggattt
    31021 gccagaaaac acgagctgtt cgattgtgcc atcattcctg gcacaaccgc tctttatcaa
    31081 tggattgacc gcggaatcat gcgaacaaag aacatggact tgcttgaaaa actgtcccgt
    31141 aaaatcaaag cggtccgccc aggacaccgt cccaacaagc gtgttctcgg gacgtcaatt
    31201 gaagaacgcc ctaaagaagt tgaaacacgg gaaacgttcg gtcattggga aatcgacacg
    31261 gtagtgggaa acaagaccaa gtcggatgcc gtgctgctga cgctggctga acggcagaca
    31321 cgctttgaag tcatcctaaa gttgaacggg aaggacaccg aatcggtgga ccaagcggtt
    31381 caacagctaa gagaacgggc tggcgctaac ttcgatcaat tatttaagac cgttacatcc
    31441 gacaatggct cggagttttc tgggctacac gatgctctgc aagatgtagc tgacgtctat
    31501 ttcagccacc cctatgcttc ttgggagcgt gggacgagcg aaaaccagca caaattaatt
    31561 cgccgtttcg tccccaaggg aagcccgatt agtgaggtga gcgagaacca gattttgcgg
    31621 atccagcgtt ggatgaacga ttacccgcgg aaaattttgg gttatgcgac accacatgat
    31681 acattcgtcc aggcattcaa gcaagaacgg ttggtggctt gacccattat tccttgataa
    31741 cgatcctcac tcgggacatg gcttcatgtc tggaaagctg gcctggggcc agaatcgtct
    31801 gaagactatc atacctcagt tcattatcaa ggcctcactg atttagaata acgaggcagt
    31861 tacggtatca aacaaatgag ttacctgt
//
LOCUS       DAYYJC010000022        31546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000022 DAYYJC010000000
VERSION     DAYYJC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.022"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1652
                     /locus_tag="ACHA9N_001005"
     CDS             15..1652
                     /locus_tag="ACHA9N_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084483.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1797..2537)
                     /gene="truA"
                     /locus_tag="ACHA9N_001006"
     CDS             complement(1797..2537)
                     /gene="truA"
                     /locus_tag="ACHA9N_001006"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9084484.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2728..3363)
                     /locus_tag="ACHA9N_001007"
     CDS             complement(2728..3363)
                     /locus_tag="ACHA9N_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9084485.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3510..4730)
                     /gene="sstT"
                     /locus_tag="ACHA9N_001008"
     CDS             complement(3510..4730)
                     /gene="sstT"
                     /locus_tag="ACHA9N_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9084486.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4949..5947
                     /locus_tag="ACHA9N_001009"
     CDS             4949..5947
                     /locus_tag="ACHA9N_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084487.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6143..7978
                     /locus_tag="ACHA9N_001010"
     CDS             6143..7978
                     /locus_tag="ACHA9N_001010"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9084488.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8038..8754)
                     /locus_tag="ACHA9N_001011"
     CDS             complement(8038..8754)
                     /locus_tag="ACHA9N_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9084489.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8981..9523)
                     /locus_tag="ACHA9N_001012"
     CDS             complement(8981..9523)
                     /locus_tag="ACHA9N_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM9084490.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9528..9755)
                     /locus_tag="ACHA9N_001013"
     CDS             complement(9528..9755)
                     /locus_tag="ACHA9N_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9084491.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9834..11714)
                     /locus_tag="ACHA9N_001014"
     CDS             complement(9834..11714)
                     /locus_tag="ACHA9N_001014"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9084492.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11903..12517)
                     /locus_tag="ACHA9N_001015"
     CDS             complement(11903..12517)
                     /locus_tag="ACHA9N_001015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9084493.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12659..13219
                     /locus_tag="ACHA9N_001016"
     CDS             12659..13219
                     /locus_tag="ACHA9N_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9084494.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13524..13838)
                     /locus_tag="ACHA9N_001017"
                     /pseudo
     CDS             complement(13524..13838)
                     /locus_tag="ACHA9N_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14092..15727)
                     /locus_tag="ACHA9N_001018"
                     /pseudo
     CDS             complement(14092..15727)
                     /locus_tag="ACHA9N_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16050..16529)
                     /gene="rlmH"
                     /locus_tag="ACHA9N_001019"
     CDS             complement(16050..16529)
                     /gene="rlmH"
                     /locus_tag="ACHA9N_001019"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9084495.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16957..18246
                     /locus_tag="ACHA9N_001020"
     CDS             16957..18246
                     /locus_tag="ACHA9N_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM9084496.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18285..18890)
                     /gene="ytpR"
                     /locus_tag="ACHA9N_001021"
     CDS             complement(18285..18890)
                     /gene="ytpR"
                     /locus_tag="ACHA9N_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9084497.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19095..19559)
                     /locus_tag="ACHA9N_001022"
     CDS             complement(19095..19559)
                     /locus_tag="ACHA9N_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9084498.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19581..19901)
                     /locus_tag="ACHA9N_001023"
     CDS             complement(19581..19901)
                     /locus_tag="ACHA9N_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9084499.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(20045..21124)
                     /gene="pepA"
                     /locus_tag="ACHA9N_001024"
     CDS             complement(20045..21124)
                     /gene="pepA"
                     /locus_tag="ACHA9N_001024"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9084500.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21324..21674
                     /locus_tag="ACHA9N_001025"
     CDS             21324..21674
                     /locus_tag="ACHA9N_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9084501.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21897..22844
                     /locus_tag="ACHA9N_001026"
     CDS             21897..22844
                     /locus_tag="ACHA9N_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9084502.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22901..24319)
                     /gene="pepV"
                     /locus_tag="ACHA9N_001027"
     CDS             complement(22901..24319)
                     /gene="pepV"
                     /locus_tag="ACHA9N_001027"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9084503.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24468..25313
                     /locus_tag="ACHA9N_001028"
     CDS             24468..25313
                     /locus_tag="ACHA9N_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9084504.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25317..25757
                     /locus_tag="ACHA9N_001029"
     CDS             25317..25757
                     /locus_tag="ACHA9N_001029"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9084505.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            25807..26001
                     /locus_tag="ACHA9N_001030"
     CDS             25807..26001
                     /locus_tag="ACHA9N_001030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084506.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26137..26823
                     /locus_tag="ACHA9N_001031"
                     /pseudo
     CDS             26137..26823
                     /locus_tag="ACHA9N_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(27231..27920)
                     /locus_tag="ACHA9N_001032"
     CDS             complement(27231..27920)
                     /locus_tag="ACHA9N_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084507.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(27895..30024)
                     /locus_tag="ACHA9N_001033"
     CDS             complement(27895..30024)
                     /locus_tag="ACHA9N_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9084508.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(30072..31409)
                     /locus_tag="ACHA9N_001034"
     CDS             complement(30072..31409)
                     /locus_tag="ACHA9N_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9084509.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
BASE COUNT         9660 a         6261 c         5414 g        10211 t
ORIGIN      
        1 aataggagaa aaaaatgaaa aaaaacaaaa accaaataat agatatcatt tttatgttat
       61 ttctaagttt tgcttacgtc atccctctat tcatgtctaa gggaatctat catagtgtca
      121 atcaggatac ttactttcat ttatctcgaa tcataggact agataatgta tggagttctc
      181 cagtaaactt caataacttc gatcatcatg gaacaatgat gaatattttt tatccatggc
      241 ttactcttta tccagctttt ttattttata aaatgatggg aaatcttgtt ttaagttata
      301 atatctacta tttttttatc acttttttaa caatggtcgt ttcttatttt tctatgaaac
      361 aaataaaaaa caatcgttat atttcgctgt tattttcgat tatttataca ttttccgcct
      421 atcgtgccat tgacatattc catcgtgctt cattgggaga agctgtcgct ttaacatttt
      481 tgcctttgat actaatgggg tgttacgaaa tttatattag agattaccaa aaatggtact
      541 ggttgtctat tggcatgacg ctagtagtgt atactcattt actttctgtc gctatggttt
      601 ctgtatttat tggaggaacg ttgtttcttt ctttttattt ttgggaccaa aaaatcgcta
      661 gactcttaag tcttttaaaa gctaccgtcc tcaccttttt tttaagtgcg ggattcttaa
      721 ttccttttat tcaacaatct agagcacaag aattgaaagt cccactggga aaagaattaa
      781 gtggtatggc tcctagtgat atgctgacac atattttaaa taataactat aacaactata
      841 ctattggatt attcttattt ctcggattga ttggtgcttt tatttttata aaaaaattaa
      901 ctgctgatga tttatttatt ttctttcttg gtgtatttgt tcttttttgt tcaaccaatt
      961 tatttccttg gcaacttttt aaccatacac cagttaagag tctacaattt gtctggcggt
     1021 taaatggttt ttcttcttta tttatagcct atacgatgag tatagtgatc tattatattt
     1081 tttctactaa aaataattca tggaaaataa tggtctttac gtttcttgca ctaatattgc
     1141 atctctcagg agtaagcaat tctatccatg ctaacaaaga ttcattaaca cttatagcac
     1201 ctgaagacgc tgttgctata gctgaaaatt acaatcatac tgattatgca aacaaagagt
     1261 caatctatca tcctgatatg attaataatg atcaatattt actaggtaac caggaaataa
     1321 atccaacaac tcattttatg agtaacaaac ttacaattga attagataat tcgaataata
     1381 aaaatactgt cttgacgaca cctatttatc gttataaagg gcaagtggct tctattaatg
     1441 gcaaactagt acaaacaaaa ttatctaagt ttggcacaac tgaattaact attcctccag
     1501 gtataaataa agtcgttatt acttatcagt acacaaaact agcaattgct tcaagatatt
     1561 tatctatcgt tactttgatt ttatttttac tttatcgatt tactttttct aaacaaaaac
     1621 aaccgagaag agaagtacaa cattctcact agatttttat ttaagaaaga ctttttagct
     1681 gtacacataa acaggctaaa aagtcttttt ttatatttct ttaatttgct tcatacatta
     1741 tttaacatcg atatttatac tgtgaaacat gcgtttcatc cattatcaaa gtatttttat
     1801 cgataataaa cttcatccaa aaacagacct tgtgcaggga cggtttctcc tgcatgttgc
     1861 cgcactttgc tttcaaatat ttcatcgatt gtttctatcg gcatggcacc tgtgccgatt
     1921 tctagtaatg tccccatgat aatccgcacc attttatata agaatccgtc tccgacaaaa
     1981 gtaaaatgaa gcatttctcc ctttcgttcg atagataatt catcaatcgt acgcactgtg
     2041 gatttttttg attttttcaa tgcagaaaaa ccaatgaaat catgtgtacc cactaatttt
     2101 tcgcaagctg cattcagctt gtccatatcc aactcttgag ggaaataaaa gctgtggttt
     2161 cgctcaaatg ctgaaggaat cacgttgttc caaacataat agctgtattt tttgcccacc
     2221 gcattatatc ttgaatggaa gcgctcaggc atttcttcga ttcttttgat gacaatatcc
     2281 cgcggaaggt aacgattcat aaagtcgatc atttctgcca gtccaatttc tgtgtttgtt
     2341 ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat ctgttcttcc tgatccaata
     2401 atttctatct cttcatttgt catctgatgc aatacttttt cgatttttcc ttgaatggtt
     2461 ttatcgtcat ctccaagtct ttgccatcct gaatatcttt ttccgtcata ttcgattgtc
     2521 aacttgatgt ttctcatatt ttagtggctc ctctgtcttc catttggtct tctctctacc
     2581 tttttaagat tgtagcacaa aatatagctc cttgcttctc taagacagaa gctatacaag
     2641 aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag ccaccggatg aagtatccgc
     2701 tgactgttca atttgacggc tcaagtttta ctgtttgaga tgcaagatac tcaacctggc
     2761 aatgggcaca taattaaaga aaaaactgat tctttggcaa aaacctattt tattcagtaa
     2821 tggccaagaa tttatagaag gaattcttgc cagtccataa atcgccgcaa aaagcaaact
     2881 gatcacagac agtccaaggt ataagtggct tacgtccatt tgcccatttt ttcgatagat
     2941 caagaaaaga agaactggaa acaataaaaa accactgtaa cctaaacctg atccgatatt
     3001 atggacccat gtcgagacat tccacgaaga ttcatgggta ttgatactga acaaacctgt
     3061 aaagatacaa tcacctatac catagaacgc gatggctgct gtaactaggg tagctaacac
     3121 acgagatgta ctaacaaatg cagcatacaa tgcaggcaat gacaggatga aaaagatccc
     3181 tgaaaccaca gaccaaataa gaaacgcttt tcttacagga ctggaaacct ctccaaatac
     3241 actgatcacg ctcttcattt gatctaattc aggataatac aatcctaata cgtacggagt
     3301 caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa ccaaatttac gtaaaaaatc
     3361 catgatacgc ctccaataac aattacctat gcttttaatt gttattataa acgactttat
     3421 cttgatttaa cagaaacctc tatttacacg aacatctata taactcatta aaaactcgaa
     3481 tcattttaaa aatactaaat atctaacgtt caacgttcac ttaatccatc taaacaatct
     3541 ttcaatttga ttttttcccc acttctttta cgcgcgccta gctctcctat tgcagtaaac
     3601 aacagatcac ttgacgaatt taaggcagtt tccattgaat cctggataac accgataata
     3661 aatccaatac cgacgatttg catcgcgaca tcattagaga taccaaacaa actacatgct
     3721 aatggaatca ataacagtga accgcccgca acacctgaag ctccacaagc agctaaagca
     3781 gacaaaatac ataaaaggac tttcaaaaca aaaggtgctt caatccctaa agaatgtaca
     3841 gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc ccatattgat cgtagcacct
     3901 aacgggatcg atatggaata agactctttt gtcaatttca atctttcagc caacatcatg
     3961 tttatcggga tatttgcggc agaacttcgg gtaaagaaag cgggaagtgc gctttctttc
     4021 aaacaaaaga aaatcaatgg atagggattt tctttaataa acaaaaacgt gaggaatggg
     4081 tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa caagtaaaag ctgcagatac
     4141 tctgccaatc cagcgattcc agtcgtagca attgattgat aaaccaatcc taaaatccct
     4201 atcggagcaa atgcaataat cacttgtacg acttgtgaca acgctataga tatctgatcc
     4261 accaactctt ttgtcgattc tgaacgtgct cgaaaagcta gtcccaacaa tacagcccag
     4321 aataagacgc caaggtaatt cgcatcaata agagattgaa caggattttg tgtgacattc
     4381 gtcaacatag tagataaaac actttccaaa ctttgcggtg ctttttcaga tactgtttct
     4441 tgtaaagtaa taggaacagt aaaaattgaa cttacaataa ctgctacaag tgcagaaagc
     4501 aatgttccca ctaaatataa gatcaaaata ggcttcacga atgtcttatt cccaatttta
     4561 tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa taggtgcgat tgccttcaag
     4621 caactgacaa acaacgttcc caatattgaa ataaatgtcc atgtggggaa aaagatacca
     4681 aacaatattc cgactaaaat accaacaaca atacgttgaa ctaacgacat atccaaaaac
     4741 ttctctaaca aatgaccacc ctttcacaat tttaacaatc attgtatcat tttatgcaaa
     4801 gaaactaaag gtataatttt ctaaaaattc taacaataca aacaaccatt tcttgctaat
     4861 tttgtacttt ttctgtctac acgttgtttt ttgagatatt ctaagttagg atgttattaa
     4921 tgggcaactt atcaggaaac gttttcttat gtttttgtcc aatttttttg tgaggtgttt
     4981 attcttgaaa aaaataaaga aggagagtta tggcttcctg acaatctttg tcgcttgtat
     5041 aatgatggtt ttacagcgta tattgatttc agcagtcact attcctacat atgcaacaaa
     5101 ccctggtcga ttctacatct atacattttt acaaacttcc gttgttattg gtgcaaattt
     5161 gatcccactt tacatgggtt accaatatca aaaaatcgcc cgtttaaaag ttttacatta
     5221 tttaagtcgt tttgccttta tattcctaat agccacacta gtttgcaata tcttttttta
     5281 cgtacaagct ggcagcttga atattcgtga ttattggctt atcctaacac ctatcagtca
     5341 gaattacttt acttatgctg tttcttgtac tttgcttttt tgtagtattc cttatacagt
     5401 tggattttta aataaattat cgaaaacaac aattaaatat ctactattat ttttaacaat
     5461 tggattagtt ggctgctcta ctctatttaa taaagacccg tttgcattac agaatggaca
     5521 gagtatttta tggatttttt atttattttt aatgggttat ggtttgaaag aatggaactg
     5581 gaaagaaaag gtccgctata agcttcctca agtatttatc agcttgattg ttttattcgg
     5641 tttgattatt ttgatgacac aaatttcact catcatcaga ggaaatacag atacagcgct
     5701 gaggttttct aaaccatatt ctctcttcgc aatgtattat actttctctt cgtttttatt
     5761 tttagaaatg ctatctgata aattaggtat aaaaggaaga ggaaattttc ttgctgtatt
     5821 tttgattgtc actcaagtta tatgtaattt ccctttagtt tcttatcgcg tatctacatt
     5881 tatgaaacgt gaatttccaa actcagggaa ggcatggctg ataaatatcg ggcagttttt
     5941 tatttgatat ttattagcag tgtccctcct agttatcttt ttattttata tacaaaaaat
     6001 ccgtttcatt catcgtttca tcgaatactt cgcttttgat tcgattgaac aattaactca
     6061 gcgttggaaa aaaagaattc attggctttt tgttcataaa agttattttt tagttgcagc
     6121 atttttttac tgctttacct tcgtgcagat tttcgtttta gaaccaaaag aaggttggaa
     6181 agaaactatc caggttgcct taaaaatatt tactcagcga caagcaccca tgatcctgac
     6241 aatcatcatt attatgggat ttttcttctt actattgttg atttccaatc gtttttggta
     6301 cgccttgact gctaccttga tcattaactt actgctcact atttcgacag taatcaaaat
     6361 ggaaatgcgg gaagagcctg tctttccttc agatttaaaa atgctgaccg gattgtcaga
     6421 actattatcg atggttagcc cagtcttatt aatcgtgggc ggattgatcc ttctcctatt
     6481 attgattact agtattattg tacaacgtag actacaaaaa cagtattcct tgaagattca
     6541 ttggaaacgt cgatttatca gtatcgctgt attattagga tgtttttcgg gtgtcttttt
     6601 cattaatcac aaaaattcgc cttctttctt gctgtttaat ttatttaaag taaacaagac
     6661 tttctttaac cagcaaggcg ccgtaaaaga taacggacca gtcattcaat tcctaaacaa
     6721 tatcgacatt aaaatcatgg aagaacctgc tggctattcg aaagaaaaaa ttaatggaat
     6781 aatgaaaaag tacaacacaa aggcagatga aatcaaccaa acaagaagtg actggttaaa
     6841 caatcagacg atcgttctca atttaagtga aagcttcagt gatcctgcac gtgtccctaa
     6901 tctgacagta cctacagacc ctattcctta catcaaaagc attataacag aaggaacagg
     6961 aggattgatg ttatccgtcg gttatggtgg cggaacagct aacatggaat gggaaggact
     7021 gacaggcttg agtatcagta acctgtctgc ttcgttagtt acaccttata cccagctagt
     7081 ggagcgtcag aaaataagtc caaatattac cgatctattc gatgaaaaaa tagccatcca
     7141 tccttttaca gctgctcttt atcgcagaaa acaagtgttt gataaatttg gttttgacag
     7201 gttctattat ttagacagtc cggataaatt aacttatacg gataaaatag aacaaagccc
     7261 acgcatatcg gatgattcag catacaaaga aaccttgaaa gctttacaaa gcaatacgga
     7321 aacgtcacaa ttcatccaat tatccactat gcaaaaccat atgccttatg atgattacta
     7381 tagtgaattg aactacactg ctgaaggaac tgctgtcatt cctaatagaa gaaaagaatt
     7441 acaaacttat atgcaaggcc ttcactatac cgatacagca gtaaaaaaat tcatccaaga
     7501 aatcgacaaa atcgataaac cgattacctt tgttttttat ggtgaccatt tacccgcaat
     7561 ctatagtggg aatgattcga aaaaatatgg attagaacag caccagacag attatttcat
     7621 ttataacaac aagtatagtc gtgaacaagc agctacgtta aataaagaag ttgttgctcc
     7681 taataacttt ggtgctatgg ctcttgagca tgcgaatatc aaagtaactc cttactatgc
     7741 gttgttgaca gaagtatcta atcatctacc tgcattaaca attgatccta cagaaagctt
     7801 gtctaatcgt tttaatggaa aacaggtctt tgttacagag gatagtcaaa ttatccaaga
     7861 aaaaaattta actgagaaac aaaaagaaat tctagaagat tatcgtctga tacaatatga
     7921 tcttgttgca ggtaaccagt acagtgcaac atgggcgaca caaaaaataa aagactaaca
     7981 aataaactga gcaaggggtt gtgataaaag acatgtcaca accccttgtt tatctcatta
     8041 atttgcttgt ccataaacga tttgattaaa gccatttttc aattcattat tcgctgtctc
     8101 gttgaagtaa agataattat cgggattccc ctgattttgt tgtgtcacca atacttttgg
     8161 ctgattttgc tgaaacccga atagatagac gtgctttttc agatatggtt ctgtctctgc
     8221 atcggaatag acggctgtga tttgatagtc tgcttgacct gtcccacttt ctgaccattc
     8281 tgcagaaaca ggcactccac caatagccat cttccactcc ccgttcaaaa tagcttgagg
     8341 gactttaaga ccatataagt ccacactcat ttgattcgta taacttttgt attgttgtcc
     8401 cattgtttgg ccccatttgg tcataaatgc ctctaattgt gcggatttgt ctgcattcca
     8461 cagtgtttgt tcaacttgtt cgctgctttc tgaagaggaa gatgaagcct ctacagaaga
     8521 acttgaagta gatgtttgag ttgtttcagt cgttttggaa gactctgacg aagtagaggt
     8581 atctgctgtt tctgtacttg ctgcagttga cttgctctct gtgctagttg ttgtagtagt
     8641 tgcaactgtt gctttcgctg tttcttttgt tgattctttt ttcgattcat tcccgttggt
     8701 acaaccagct aacaaaaaag ctagaccaat tgttactaaa atcctttttt tcaattaaaa
     8761 aactcctttc gattactccc acttctttat tctatcaaat aagcaatgtg attttattga
     8821 tttttcgtat tttctcatca atctttcaaa tcttctaaaa actgagaagc acccgctgat
     8881 gttgtctgta atacatcagc gggtgcttct tttattttaa tcttcgtgat tttccaattt
     8941 acctcacgct ttatatttgt atttgaagaa acatagactt ttagtcatca tcgtcttcct
     9001 catccaaatc ttgtcggaca gtttttcctt ggagtctttg tacttggtag atgatttctt
     9061 tattgaaact taaaagttca acaagcaatt gttgcaagcc atatttttct tctttttcag
     9121 ctaatttgat tgcccgtgtg ataaagacat tttggaaatc aaaatctttt gcaaagtttt
     9181 ccaacattgt tgaggctggt aaatatttgt tttgaccatg ttcgtaaatt tttccataac
     9241 gcatgacttc ttctaaagta gacggaacga tttcattttc aatcactaac tcatgagaga
     9301 ttttattgaa ccatctcgct tcttgatcag catattctga aaaaagggta cttaattctt
     9361 gcgaagatgt acccaaaaca tacaattgtg cttggatcaa tttcatttct tctaatttaa
     9421 aatttgctaa tacatgcccc accatggcgc cagcagttgg tttatgatgg tcgatatctg
     9481 cttgttttgt ttcttgaaca aatttttctt ccactatatt cattgattta cgcctctttt
     9541 acttttgctg attggacttc ataacctaat ttttccacaa ctgcagctaa ttgttcaggg
     9601 tggacttcat ctggatcaaa gtttgctttg attttgctcg cattaaacaa aactttcgta
     9661 ttgcttaccc caggctgttg agaaaccgca tgttcgattt tttgcataca gcttggacaa
     9721 gctagtgtat ctaattgcat aataattttt ttcatcgtag ttcttccttc ctttttcttg
     9781 atagtcttat tctagattac agcttccata aaaaaattga ttcttatcaa gttttacgat
     9841 ttttcttctt tgctttcgtg tccaaaatac aataaacgca tcgcgttaaa gataacgatc
     9901 aagatactag cttcgtgaac gaacatccca ctagccatgt aaataaaccc ggcaaataag
     9961 ccgttgagta aaaataagac gacaccaatg gcaatcacta cattttcacg ggtatttaaa
    10021 acagttttct tagccaagcg gtaggcatga accaacgatt caaaattaga ttgcatcaag
    10081 acaacgtcac tcgtttcgat agcaacatcc gttcctgatc ccatagcaat cccgatatca
    10141 gccgcagcca aagagggaga atcattgatt ccatctccaa taaaagcaac gcgcaatcct
    10201 tgttcttggt attttttcac aaattgaacc ttttcgtctg gtaataattc agcatggact
    10261 tcatcaatgc ctaacatatc agctacataa tcagcagtca tctgattatc acctgtcagc
    10321 atcactaact gttttgctcc tgcttgtttc aacttagcta attgttcagc gacttctggt
    10381 cgaatcacat ctgacacacc tatgatttga gtgatttttt tgtcgattgc tacaataatg
    10441 accgagctgc ctgtttgttg taattgatgt aaatcttgta cttgctttgg tgtgagagaa
    10501 agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa ctgtatgacc atcaatctca
    10561 gctgtgattc cttgtccctt gattgtctga ttatctttga ctgctaattg ttgaaaatca
    10621 atgtctttct cctgagcata atggatcacg gcgcgcccca gaggatgatc ggataacgtt
    10681 tctaatcgag ccactaaagc taagctgtcc ccttcatcct ctgtatagtt cttgatagtg
    10741 gatacagcag tagttccttt ggtcaatgta ccagttttat caaagacaaa agtatcgatt
    10801 ttggaaaaag tatccattgc ttcaccgcct ttgactaaga tgccccgttt cgctccatta
    10861 ccgattcctg ctacattcga aacaggcgca ccaatgacta aagcgccagg acaaccaaga
    10921 accaaaacag tgattgctaa acgaaaatca cgactaatca gaccaacaac caacgcaata
    10981 agtaaaacgg ttggagtata atactgagaa aatcgatcaa tgaatttttc cgcatgggat
    11041 ttggtatctt gtgcttcttc tactaattcg ataattttgg caaaggtcgt atcatctccg
    11101 accttagttg cttcaatcgt cacgtagcca tctgctaaaa tcgttccaga gaagacttca
    11161 tctccactga ttttgacgac ttgtcgtgat tcaccagtca ctgcggcttc atccgcatac
    11221 cctttcccgt caatgactcg tccgtccaca ggaatagctc caccagtttt tactaagacg
    11281 atatcgcctt cttccacgtc atcgatttcc acttcttcag tcgttccatc ttcttggaca
    11341 acactagcag tagatggggc catctcggtc aaagccttga ttgactcccg cgttttggtc
    11401 aatgtttttt gttccagata gctaccaaac aaaaacaaga atgtaacgat agctgactcc
    11461 ttgtattctc cgatcataaa ggcaccaagc acagcaatag taacgagtaa atcaattgag
    11521 ataactttga ctttcactgc ctgatacgca tggatcataa taggtatgac cgcaataata
    11581 gaagctatgg ctagagcaat gttatagaac aagacaaaac cagtaaattt tccgataaaa
    11641 ccaaaaataa tcaacacagc tgataaggcg gtgatatgat ttcgatgctc ttggatccac
    11701 ttttgtaatt tcatctttct ccctcttttc ctttcattct tacgagacta agtatagata
    11761 cttatccatc ataaaaaatt gaccactatc aagtttagtc aaaaaggcgg ttttcttttg
    11821 gtagaataca ctacaaaaaa tagttagcaa gaagactcac taaaagtgat cttgccaact
    11881 attgtcgttt ctatcgaaat tttcattgaa tgattttcct gtacagcata catcggagat
    11941 ctatttcttt atctaagatt tttatcttgc taattttttc ctttagtctt tcaaatccac
    12001 gtcgttcata gaattgatac gtacagccac tatctgtaaa aaggaatatt tcttttcctt
    12061 tctctctttt ttcaaattct gttagcaact tggtaccgat gccttttgtt ttcaaatcag
    12121 gatttgcagc taaaaaaatg atctctccat ctggcgaatg cttctttaaa tatgctgcaa
    12181 gcatttgttc atttgctgtc tcgtaagctc ctttactttc tttagcaaat atattttgta
    12241 aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga acgatgtttc tttttttctc
    12301 cttttatctc cgccagcaaa acacctgcca actcgtcctc tgcatacaaa gcaagaattt
    12361 gagttgctcg attcaactcc atatacaaaa aatatcttcc gtaaagattc agcagaagtt
    12421 cattttccat atatctctca aagttcattc cttcaacagc aaaatagata gcttttttgt
    12481 aatctttctt ttctaagggt ttaaaagtta gtttcatcat ttcgcctcct caacttcaat
    12541 gattgtgtta tacttacatt ataatagaca cagtgtctac tatctataat caatcatcta
    12601 taagtttgtc gattaagcga cagctttgct ttgatatgtc ggataaggag ataggtaaat
    12661 gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct ttggctgaaa cactatccac
    12721 attattagta aataaaaaga ttcaagcaat cactatacgc gaactaacag aaacggcaga
    12781 tgtacatcgt tcgacttttt atacacatta taaggatatc tatgatctat atgatcaatt
    12841 agaaagcaac ttttttcggg atctaaatga aattctctct tatgatccag cccatagcta
    12901 tgaggaacta tacactcgct tgattgacta tgtttacgca aatacttttt tatatcgctt
    12961 atttatcctc aaccatggtg atatttcgtt tcagaagaaa gttaaacaag ttattgaaga
    13021 aaactatctg aacatatggc tatatgaaga caagaaaacg attatcacag aagaaatgag
    13081 attctttact acgtaccacg tccaaggttg tctatccgtt ctcaatcggt gggtagaaag
    13141 caattacaag tactccaaaa aagaattatt agaactctta cagaaactga atgatcacat
    13201 agagaaaatc atgccttgaa taaaaacata ctaagcttag ttaagaagaa ccgtaacctt
    13261 ctcgtataac aactgaatct tatttagcca aattgtttac tgaagatttt ttgagtaaaa
    13321 tttctataat tcctacttat tgaaaagtag tttgtagtgc tgcttctccc tctctaagtg
    13381 atatatcttg ttgtaccaat caaattcacc tcatcattaa taaattactt ttaggctaat
    13441 ttattacaac ttataggttg aagctgtaac caatttatag tcatattata tttttatcat
    13501 ccagtttatt gaattcttct cgtttacctc aattcatttt caaggggaat cagctcatca
    13561 tctaacattt tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg tttcgcttct
    13621 ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga
    13681 attggatctt cacctggcat atattgcaga ttagaagcat gtaatttttt ctcagaaaca
    13741 aaccactcat tggaaaagac atgaatcaaa tcatcactta cttgtgtaaa atagcgacgt
    13801 ttaatgacaa agatatcttc attgctatct agtgtacctt catttctttg tgcttaaatt
    13861 caaagataat tttctcaata aagatttttg attccagtaa gattccttta ctccttttac
    13921 aacatagcgt tgtcctttgt tttgaaacat cttcttcccc tctttgccat taaaaatagc
    13981 ccaattctct ataaagaaga ttaagcttca ttattataat ataaagttta aatttggttt
    14041 attacttact ttttcttatt ctctagtaag ctaatattga aaaaaatgta atcagatcat
    14101 tatttttata tgttgtaact tcctgataag aaaactaatt aaaagaaaga ggtaactaca
    14161 taaactaatt aaaaaagcta acttcatagt tggaggataa atggaagaaa cttcaatgat
    14221 tgtttctcct tgtgttcctt caatcgacac tagtcctatt atcaatctct cttttttgtc
    14281 catttacttt tgcgactgtt ccccaataat caaatacagg aagcgttatt ttttgtcctt
    14341 tagattcatg tactgtatag actattttcg aatccatcac ttgataaggc atagtatgcc
    14401 attccttgcc aattcttact tcatgattct gcaaatgttc tttgtattgt aatgcttccg
    14461 tgggtgcata gtcataaagt cctccgggaa ggagtgtcgt gtaacgaata agattttctt
    14521 gattcattac caagtgatct ctctgagtga caactttatg ttcatagttt gcttttgttg
    14581 cagcagatac agccaacagt aataaaacaa ttgccgttat attaactact tttctttgct
    14641 tagctgatgt gctcttcatg aaaacgaacg ataaaatcaa cgagccaaac agtacttgaa
    14701 agcctaagat tcgccaagga aattgaatca tttgaatggg tgtattttgt aataaagacc
    14761 atggaaaaaa tttggtggat aagagccaag agactaccgc actaaggtaa atcacttttt
    14821 ctttgcgatt aaatccttta aaaaagaaga gtggacagat caaagaaata ataccaatga
    14881 ttcctaccgt aaaggttgtc aggtcattat tcatcgcacc aatcaaacta tccgagacat
    14941 ttaatgcttg gtgttgtaag gtcgttttat ctggctcatt aatcgattga accatgattt
    15001 gtcggaacat cggataccaa aagtatgctg ttaaaagaat cgtcattcct actgcctttg
    15061 acaactccac aatcaaccgg atgctttctt tttttcttgt ttcacttact acaaagccgc
    15121 ctaagactag taagccaacg gctattacac tcataaaagt ggaaagaaca tgtgtataaa
    15181 tgagtaagct catactgact gctaatggaa ttgcacatgg ctttttctca taaatcagtg
    15241 cgtaagcata atacataact atcggtataa ccgtaagtgc aatcgcttca gcaatagcag
    15301 aacgatgata aatatcaatc gtacggtacc cataaaatgt atataaacag ctaaatataa
    15361 aagccgcatc gattcttttt aaaaatttta gtccataatg aaacatcagc atgatagtac
    15421 aaacattcaa tagccaaaca tataagatat aactaacgat taaattatta cttatccaat
    15481 aaaaaatgac tgccggaaaa aaggtcaagt agggataaaa ataattcact ccgctcccat
    15541 aatgattaaa cgtcgtaaaa ttgattggac cagaaaaaat tgaagataac cctttgactc
    15601 tagcaaaatg gaaggcaata tcccccgatg tagaaatagt atgaacattt ctaaaataat
    15661 tgattgaata aagtccagca ataattgcta ggagaatgga caatattatt tttttcttta
    15721 cttgcattac ttaattatat actcctttca tggattgctt tatctagtat aacttaaatt
    15781 ttaaataaat cgttatttat ttttactctc acttaaaaaa gcactaaaat tataagagag
    15841 ggaggtaatg gtctgaacac caagaattgt tccaacatcg aattgcttaa ctaatgagtt
    15901 caaagcttaa ttatgtggct cttatattta ccgagcgtgg attgcgatct ctttagcgaa
    15961 acaaacatct ttcataaaaa aaacagccat atccgctttc ttggatatga ctatttacta
    16021 ctactttact atctttcatt cttcttgtct cacttatgat aaggcgcccc cgcattgatc
    16081 cggaaggcac ggtagatttg ctcgacaagg atcagacgca tcaattgatg tggatacgtc
    16141 atttttccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
    16201 gagccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
    16261 ttagcaaaag attcacttgt aggatttttc ccttcaattg ctaaggcata gacgtattct
    16321 tgttctttga ttttcgctaa gatccgttcc ccttcttttt cttttacttg tctcatttgc
    16381 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gatttttgca
    16441 taggcattca gccgtttgac atactcattg ataccttgga tcaggtattt ttcttttaat
    16501 tttcctactg agataatttt gatattcatg ttttctctcc tttggtcttc atgctcactt
    16561 tgtttattat aatcaatttt ttcgtctata tgtttatttt ggctattttt gttaaaaaga
    16621 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag tgttatttta
    16681 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
    16741 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
    16801 agtatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
    16861 tttttcataa aaagttcaca gtctcaaagt agactaaagt caacaaataa gactatacta
    16921 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
    16981 aagatgaaaa aaagtaaaaa taatagcctc tggcgtaagt taggtctggg gcttgttgga
    17041 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
    17101 aatagttcta ctgctacaag cggcaatcag aattctgccg gtgaaacagt tgtggaaaat
    17161 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
    17221 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg actattcggg
    17281 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
    17341 ggcgtgatct acaaaaaaga cgggaatact gcttatgtag tcacgaataa tcacgtagta
    17401 gatggccagc aaggtcttga agtcttgatg aaagacggta cgaaggtaaa agctgaatta
    17461 gttggaacag atgcttattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
    17521 acagttgcct cttttggtga ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt
    17581 ggttccccac taggatctga atatgcaaat tctgtcactt caggaatcat ttcttccttg
    17641 aaccgacaag tgacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
    17701 acagatgcag caatcaaccc tggaaattct ggtggtcctt tagttaatat cgaaggacaa
    17761 gttatcggta tcaactcaag taagatcgca agtacttctg aatctacttc taatgtaagc
    17821 gtagaaggaa tgggatttgc tattccaagt aacgatgtag tgaatatcat caatcaactg
    17881 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
    17941 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
    18001 atcgtgacaa atgtccaaac agctacaccg gctgaaaaag ctggattgaa acaatacgac
    18061 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
    18121 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
    18181 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
    18241 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatgct
    18301 tctccgacta cggcatcctc aggcaatact aagattcctt tttttgctgg agcatttggc
    18361 aaatgcaatt cacgagcgga acagatcatt ccatagctat ctacccctcg caaagttcct
    18421 ggccagatca tcattccatc tggcatcata gctcctggtt ttgcaacgac aactttttgt
    18481 ccagcttcga tattgggtgc accgcaaacg atttgcaata ctgtaccatt gtctacttct
    18541 gtctcagtaa tcgataaatg gtcagaatca ggatgtggga cgcatgtttt tacgtacccg
    18601 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttgatc
    18661 aatcgattca atttcgctaa ttcctcatct gttaagctta cttggccgtt cccttcgatt
    18721 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
    18781 atccgagcaa cattccattt gcgttcaaca gtgttttctg ttcctttatc gtcggctata
    18841 ataaccatca aggtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
    18901 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgaaattttt
    18961 ttcatgcttt atttccgaag aagcctcttc tggaacacca tttattcgta acaagggact
    19021 gtgacaagac ttttgtcaca gtccctttac tgttttagtt aattgattaa aaaggtcgta
    19081 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
    19141 caaggtgaat gacgtatcac gtagctggct acacttcctg tcataaagcg ttcgactgca
    19201 tttagacctg actgcccaac catgattaga tcgatctcat atttttttgg caatttgact
    19261 gccatggatt cacgaacaga accatatgca ataattcctt ctatatttgt gtaatcaaca
    19321 gatttcccat attctttaca ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg
    19381 actagatttt gatcaatagg tgcatagccc ataaagttat aaaattgctg atcgattacg
    19441 tttgctacat atactttccc attattacgg cgagccactt ctaccgcttt tttgaaagca
    19501 gcaagtgctt gttcactacc gtcgatacct actaaaatat tcttatattc ttgattcatc
    19561 ttgacccctc ctaacagatg tcagcctttt gttacacttt ctaggaacga ttcgatttcc
    19621 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
    19681 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
    19741 tctacttcaa taaattgcca atcagaaaag tcattttcaa tttcaggcat ttgcggtttg
    19801 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactctt tccagtttct
    19861 acgtagcctg ctaattcttc taaactattt gggataatca ttctttgtcc tctactttct
    19921 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
    19981 ctttttataa aaaaaatctg gcaaaaagca atcttttgcc agattttata gtttcactat
    20041 cgcattattt tccgtatacg atcgtattta ctgttgattt atccaaacct ttcagtgttt
    20101 gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat
    20161 ggatataacg tcccactacg ccaatcactg tacttgggat tccttcgttt tgtgtatgag
    20221 cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggata ttgtttgttt
    20281 cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga
    20341 tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat
    20401 ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt
    20461 tagaaccacg caagcctact tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta
    20521 atgtttcatt ttgcaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt
    20581 cccatgattt actgataata ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa
    20641 cgattgtgtc tcctggaagg acaccgtaac tcattgcctc ttctcttgat tcaaacccag
    20701 catcgaacaa aatatcggtt acttctactg atttttgtcc gcttgttcct cgcaataaat
    20761 gtgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa
    20821 aacgctgagc agaaacaacg taaggattcc agccgcctag aggtacgact ttaaaaagcc
    20881 cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta
    20941 cacggggtgc atccgcttct tcatgatggc gcagaccaaa aatgccgccc aaaccatcta
    21001 actgtaactc atctaccagc ggttccatat gactttccat ataagcgcga acatcttgtt
    21061 caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt
    21121 ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg
    21181 ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag
    21241 cggttattta tttcaaagaa aatatgagag tgtggtaaaa taagcacaag gagtaacaag
    21301 tgagaagaag gaggtatgtc cacatgtatg aagaaagcga actaagctat tttaaaggcg
    21361 ggttagcagt tggaatcggt ctaggattat tgagcggggt agcttcaacg atttggtatc
    21421 atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa tatcaagtca tcttttctaa
    21481 aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa ggaacctatt aggaaattcg
    21541 ccgttcggtc gagtgcttat cggggaggtc tttctcgaat agaagatggc gaaactgttt
    21601 attatgaatt tttagcagat gctcatacag gaaccgtatt agaaatttca agaaaaaaag
    21661 gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat acgcacccac tattcaaaag
    21721 agaggtatca tgtccttctt ttgtagtggg tggtttttta gatagagctt tccaaacata
    21781 tattccagta ttttcttttt aaataaatga tatttagcgg gaaaaactag catttccttt
    21841 gattatgagg tatttttaat ttagcttttg ttttctatag ttaaggagag tgaaaaatgg
    21901 aacaacaaga aaaaattgct gttctgattc cttgttacaa tgaggaagca acaatttcaa
    21961 cagtcattgc agactttaaa cgagaattac cagaagcaga tatttatgtc tatgataata
    22021 attcgaccga tcgaacatac gaattggccg ttcaaggtgg agcaatcgta aaaaaagaac
    22081 ctcgtcaagg aaaaggaaat gtgatccgcc aaatgttctt tgatatcgat gccgactatt
    22141 atttaatggt agatggcgat gatacttatc ctgcagaagc tgtacatggt ttgctcgaga
    22201 aattacgttc tggtgaagca gatatggtca ttggtgatcg cctatcaaat ggaacctact
    22261 ttgatgagaa taagagacct ttccacgatt ttggaaataa ccttgttaga aatacaatta
    22321 ctagaatgta taaaacaaaa attcttgatg tcatgaccgg ctatcgagga ttcaatcgta
    22381 tctttgtcaa aagttttcct attatgagtt ctggttttca aatcgaaacg gaattgacga
    22441 tccatgcttt agataaaaaa ttcaagttcg ttgaaatccc aatcgattat cgtgatcgtc
    22501 ctgaaggcag cgaatcaaaa ttgaatacat tctctgacgg attcaaagtg atcatgatga
    22561 ttattaaaat gtttaaagat tataagcctt tgcttttctt tgggatttgg accatcttct
    22621 tctttttatt tggattggtc acggggattc ctgtgattcg cgaatatatg ctgacaagct
    22681 ttattacacg catcccttct gcgattttaa gtactggatt gatgacttta gcacttcttt
    22741 cattagtaac agggttgatt ttggatacag tcgtcacgaa cgctaagaaa gaatatgaat
    22801 tgaatctata tcatgtttat catgaatacc acagtagaaa gtaaaaaaag gatcaagact
    22861 gcaaaaataa tttgcaattc ttgatcctct ttttaattaa ttatttcgcc aaacgataga
    22921 tggcatctgc atagattgct gttgctctga acaagtcatc aagactcata aattcgtttg
    22981 cttgatgcat tgtatcagta tatcctggga acatcgctcc ataagcaacg ccacgtttca
    23041 acaaacgtcc gtaagttccg ccaccgatga tttgttcgta gcctttttct ccagtgtggt
    23101 cttcatagac ttgcaataat gtagccacca atggatcatc cattggtacg taatgtggtt
    23161 ccattacgcg agcacctcga gtaactgttg cgccttcttg ccccactgtt tgttcgatgt
    23221 cactttgcaa tccgtctact gttacacctt ttggaaaacg gaaattcata ttgatgaagt
    23281 tcgcttcctc tgtaccattt tcttcaaaag caaatagtcc agcattcatt gtcaatttgc
    23341 ccattttttc atcttcataa gcgactccta gtttttctcc ataaaaatct tcatggacat
    23401 aagcagctgc tacatggata aattgtttag cagatcctag gaattcatag ttatctaaga
    23461 acaatgccaa gaaagaacct gcattgatac cagattgtgg gcttgcacca tgtgcgcctt
    23521 taccaactaa ttcgattttt acatacgtat tgtccgcttc aatcgttccg cttactggat
    23581 ttgcttcgat gaattggtaa aatgcttctt ccatcgcgat agctgcatct gcacttggta
    23641 cttgaagtgc tgcagttgct gttcctggca ccatattttc gcgaagtccg gaaacaaaag
    23701 atttcaagac ataatctcct gcattgtcgc ctttgaagcg taaagctaaa gaaacatttc
    23761 ctttttcacc attgatgatt gggaattcag catctggtga gaaaccaaaa tctggttctt
    23821 cttcatgttc gaaataataa gccatatcgc cccagccgct ttcttcatcg ctgccgacta
    23881 cgaagcgaac tttttttgat agtttcaagt caagttcttt aatgatcttc atcgcatagt
    23941 aagctgccat acttggacct ttgtcatcgc ttgatccgcg tgcaaagatc tttccatcct
    24001 tgattactgg ttcataagga tctgtgtccc agccatctcc tgcaggaacg acatccatgt
    24061 gtccaaatat ccctagcgtt tcgtctcctt cgccaagatc aatatgccct gcatagttat
    24121 cgacattttt tactttaaat ccatcacgtt cgccataagc taacatatgt ttcaatgcgt
    24181 cgcgaggtcc aggtccaaat ggtgcatctg gtgttacttt tgagtcatcg cgctcgctgt
    24241 tgacacgtaa aagatttttc aagtcttcaa gcaagtcttc tttacgtgct tctacttctt
    24301 tttgccaatc aattgtcata aagttaatcc tccacttatt taagtcttcg tgttcatgat
    24361 accacaaatt acagaaaaaa atagttcaaa aaatctgaca attccttttc ttatctgttt
    24421 ttctctaaaa aaaatatacg ctataatgag taaaaggagg tttttagatg ctgataaacg
    24481 aacaactagt gaaaagagtc atcacacctc gtccaaaaat ttcacataaa ggaacatttg
    24541 gacgtatcgt tttgattggc ggcaatgaac aatatggcgg agcaatcatt atgagtgctt
    24601 tagctgccgt acacagcgga gcgggtctca cgactgtagt aactgatgaa aaaaatcgga
    24661 gtgctcttca cagtcatctg ccagaagcaa tgattgtagt ttggacggat actgaccagt
    24721 ttctttccgt aataaagcaa gcagatgtac tcttgattgg tcccggattg ggtacttctt
    24781 ctaagagttt ggaacgtttc aaacaagtac tagaaaacca atcaaaagaa caatggctgg
    24841 tgattgatgg ctctgcttta agtttacttg ctgctaatca tcttttgcta ccctatcccg
    24901 aacaaactgt acttacacct catcaaatgg agtggcaacg gttgagttct ctaccgatac
    24961 aagaacaacg ggataaagaa aacaaaaaga agcaacagga aatcggtgct gtgctcgttt
    25021 tgaaaagtca tgagacaaaa atttatggtc accagcactt ctacaccaat cctttaggtt
    25081 ctgctgctat ggcaacgggc ggcatgggtg acactttagc tgggatgatc acaggatttt
    25141 tagcccagtt tcctcaaaat gaccaaacga ttgcagcagc tatttatctt catagtttca
    25201 ttggagacca gctggcaaaa gaacaatata ttgttttgcc gacacaaatc agcgaagcac
    25261 ttccgaaatg gatgaattat ttttgtaaaa aatcaatgga aaacgaggca tgagtcatgg
    25321 agaaatttac aggttatgtg cgagaacctt tttattatga aacagatcaa atggggatta
    25381 ttcatcacag caattatatc cgctggtttg aagaggctcg ggtagcctta ttggtacatc
    25441 tagggtttcc ctataaaaaa atcgaagaat ctgggatcat catccccgtt ctggaagtaa
    25501 attgcagcta taaagaaatg atccattacg gagaaaaagt gagcatccat ccaactatcc
    25561 aaaagtatac ggggacacgg ttggatttta gctacgaaat tgtgggaact gaagacaaaa
    25621 aattaaaaac tactggcagt agtaaacact gttttttact ttctgattcg caacgattag
    25681 tgaagctgag caaagtcaat ccggagttag atcagttatt ccaagcgtac gcttctttgg
    25741 atgatgtaaa ttcttaataa agaccgtcta agcatttttc ttgtattgtg tcgagtagtt
    25801 cgtataatga aaagaaaaaa gcaggtgatc cccatgtatg tcgttaaagt aatgcacggt
    25861 tacatcgata aaactggctg tcgcactcgg gaaaaaaact tagacaatct gctcattttc
    25921 aaagacaaaa aagaatccga agcttttgct aaaagaatcg gcggtcgtgt caaaccgatc
    25981 caagaggtac gaccagattg attcaatatt tttcttttcg aggctctaat aggcatgcct
    26041 atcagggctt tttttgcaga ctgtgttttt atgcccacac gatttgtaag cgatttatgt
    26101 tataatagat tatatctgca tagaaaggaa ggttaaatgg ttatcaataa tttgcaacag
    26161 caagcttatg aatctatacg taaacaaatc atttattccg agttagcacc tggaagtaaa
    26221 atctccgata aagtgttaga agaacaacta gctatcgggc gtacaccgat tcgagaagca
    26281 ctgatccaac tgcgcaatca agagcttgta aaaaccattc cacagtccgg cacttatgtt
    26341 tcaaaaatcg atatccgatc tgcttcatta gcccgttata cgcgggaaaa gttggaaaaa
    26401 ccgatcctgc aagaatgcag tgcgaaaatg accaagtaag atcaagctaa actagaaaaa
    26461 atactagaac aaaccgatca ggcagtaatc gctcaaaata aaaaagcttt ttttgatttg
    26521 gatactgatt ttcaccgaac ttgttatgaa attgctggaa aacgagaaat ttgggattgg
    26581 ctagaaagtt atagtacgca cttgaaccgt ttcagatggt tacgtttgac gatttcagaa
    26641 ttagactggg gtcgagtatt agatgaacat cagacaatgc ttcaatcaat gattgatcat
    26701 aactttgatg aagtcggatt tctttgcacg atgcatttac atatgattat cgaggagcaa
    26761 gaatacgtga ttcataatta ccccgattat tttgaagata ttcaggacag acccatcaaa
    26821 taagaggttt tggaatcagc gttttgacct gagtattaga gaaagaaacg gcaaaatagt
    26881 tcctcatatt ttgccgtttt ttttgagcta ataccgaggg tcagctgatt gcacaccgtt
    26941 tattgagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaat
    27001 agcttcttat gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc
    27061 gttttccaag aggtcgtgag cctgccgttc atctccgagt cacaaggaac aatttcaaaa
    27121 aacagcttct catgtttgtt gaatattttg acttgtgacc gaggagttga caggagaaca
    27181 ccgtttattc ggaaaaaaag gactgtgaca cgacttttgt cacagtctct ttatttgtaa
    27241 tctaaatcat actccgtaca tcttttttct atttttttta tttcttctaa gatcccttgg
    27301 ctacgaattt cattatatgg ccaaagtaag actgcctcta atacataagg cgaaaaatcg
    27361 ctttgattca tctcttttga aaaactcatc gctgctatct cgctcaatgc acgaatgttt
    27421 gattgatgtg tataaaaagg caaccgccac agcacaattt tttcgctttg cgtataaatt
    27481 cccttaacag aagcttcgat gtggtgaaat agttcatgtc caataatcaa agcttgcgct
    27541 gtttctttag agatcgaagc tagtcctgaa cattttaatt tttctaacgg ctccgtcatc
    27601 aatcgaatcc cttcatcttc tgcataagtt gcaaaagtga ttcgttcgtt tatttgctct
    27661 gattcacagg aaacgattgg aacagccatt tgttcacaaa tttctcctac atcggcaaaa
    27721 gggtactttt ttttgatgct acatgccgtt tcgataccac aatcgattga ttgttgtata
    27781 agaattttct gcttggacaa aggaatcttt ttccaaaata aatctcgccg aaactggtag
    27841 aatccccaaa tttcttcggg gatctgttgt aattcgttta gtaactcttc taacttatgt
    27901 ttgtttgata tttccggcaa cgacgatcac ttcttcctcc ggttccatag tagatacttc
    27961 cagatccatc aaaagctgta gttgttccag atccgtcgct gtaaaatcag agactcttgc
    28021 tcctaagcag agataaaatt ctggtcgggc aatcaattca gtttgataaa ctgccatatc
    28081 ttcccaaagt tgttcgacag ctgtcatata attggctgca gttgttttca tacatgaagc
    28141 atgtccacgt tggaccttga taaatccttt ttgatcgatg atccgaagac ttccagcatt
    28201 ttttgaacga ttctgttcgc cgcatacata aaagaaaggt gtattttcca ataagcaaac
    28261 ttcattttta tttaatcgca tatcctctgc agcaagctct agtgcttctt ctgttgtgat
    28321 ctccttggtt agatcggttg ctttcacttc cgtagaacca gtagcgatag ccgttacttt
    28381 tgaagtctgt ggatcgattt ctacgtggac ttcgatcgat tctggtgtag cgcctgattc
    28441 aatcgcttta ttcatcgctt catttttcaa cgatcgtata tcttctttac ttggcgaagg
    28501 gatgatacgt tctacaacat ctcggaccat tgcaagtgct acaccaatag aagaaatcac
    28561 ttcggcgttt tcaggaatcg aatatttcac acccatttta ttactaaagt aagtaatcaa
    28621 tgaggcagcg ccgcccccta cacctacaag agaaatctga tctttctcca agtgatactt
    28681 gtctgccaat tctaaaatca ccggttcaat cttagcatag gatttttcca tgatctgttc
    28741 tgcgatgtct tcaaccgtcg tatgacaata atcagctaat gcttggatgg cttttcgtgc
    28801 agatgggaca tttccataag aaaaatgttc ttcttgcact aatccaagga cattggctgc
    28861 acatgtattt gtaatagtca cttcttctcc gtcttccatc actaccttga cgtaatcagc
    28921 agggtctcct tctttaggag agaaaaattc aaccttagga cctttgattt tttctgtctc
    28981 cgtaaatact gcataatcta gaccggcaat atgtgcagaa cgtggaccga catcgatgat
    29041 tcctgactga ttagcccgta ccatcgaacc gcctgccaca cctagaaccc gaacatctaa
    29101 tgaagagata tatgttgggt ggccacctac aattgaataa tcgattgctg gacgtccatt
    29161 tttaatgacc ccgatattgg ttgttgttcc gcctacttca aaataaacac cattagacgc
    29221 tctcaagtac atcaaagagc ccatgactga tgctgctgga ccggaaagca tggttaaaac
    29281 aggtcgtttt ttcatttcat tgatttccat tactccgccg tctccgcgca taatcatcaa
    29341 agaaacattg acccctgctt ctcgtacgga atcttctgta gaggttgccg tatctagcat
    29401 ttttggtaaa atagatgcat tgattgctgc tgtccgagtt cttcttgtca atccatataa
    29461 ttttgtgatg tcagaagcca tcgttgtagg gatcgatttt accgatgctg cttcataaac
    29521 aactcgttct ggttccccat tatctactcc aaatgccata gaagaaacca agacctccgc
    29581 tctttctctt tctaaagaag aaatcgtttc tgaaacacgg tctacattta agtgtttgac
    29641 aggtaagaat gcattgacaa tctctatttt tttcttattc cctaaatcga tatcgttcaa
    29701 tctagtttgt cgttttgcta aaaaaccttc taatccacct tttgccatcc cgattacgcc
    29761 gacttttgct acgtctcctt caatcagtgc attagtcgct tgtgtggtag aatgtgcaac
    29821 aaacacaaca tcttctggac tgatattatt ttccagcaaa caattttgaa aagactgaac
    29881 gacacctgcg gctactccac ggacatcatc gtgggttgtt ttcactgaag attttccgat
    29941 gatttcgtgc gtcgcattat cgattgcgac tgctttcgta tgtgtaccgc caacatcaat
    30001 tcccattcgc acaaaacgtt tcattttctt ttcctccatt catctaatcg tttttatcga
    30061 cgttttgttt actaggcgtt cccgtacaat aagaacgtga tcaggcataa gatcgctccg
    30121 atgatccaac cagttgggat ggatttcttc atatattctc gagaagttac ttttgtataa
    30181 cctaatcccc aagcaaccca agattgtgta atatctaaat gctgaggagc aatcgttgtg
    30241 attgcaaata acgggaacaa aaaggctggc gacatcgttg gattaacagc taatacgaca
    30301 gctaaaatag ctgagcctga accgactaga ttcattggac cgcggaaaaa tcctagtggt
    30361 gtcaatatag caaatacgat tgccaagact aatggtgatt gcggtatcca atctccaaaa
    30421 atagctgaaa aataaggaga agcatacgca gctgaattat tgaacatcgc taaggtcatc
    30481 aagaaaccaa ccattggcgc aacatctact gcgccatctg taaataattt cgcaaactga
    30541 cgacagattt ctttataacc tccgcgcaat tttccagtaa acagcaatgc ccagatacct
    30601 gctagaataa aacctaagat gattggaaga tctaaaagaa cgacccctaa aactggtaat
    30661 aatacggcta accatgagat cattggtgcg tcgccacctt cagctggtac gtttgctgcc
    30721 attgcatagc gtttcttttt gttcattgat atattagcga ccgttagtac cacaactaag
    30781 cttacaatca tcccaatcat accaatttgg aaatagtcat tataagtata gctttcagcc
    30841 gctggattga aaccagcata aatcgtttga tattgtttga aattgacgat attagcaaag
    30901 attccagcca taatcgagcc cataaatgag aacagtgcaa ccggtactga aatacctaat
    30961 gacatcaaga ttggcaacac aataactgca atagaaatca ctggtccgat tcctgtcatc
    31021 gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct tggcttgtcc
    31081 cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc tactagaaca
    31141 cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta ctcagctggc
    31201 cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat cgccgggttt
    31261 ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat gatggcaaag
    31321 ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa tgagataact
    31381 aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg tttttctgat
    31441 aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc tttcattaaa
    31501 gggtgaccat aaatgttcag ttttcaatta aaaaacctta attatg
//
LOCUS       DAYYJC010000023        31233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000023 DAYYJC010000000
VERSION     DAYYJC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.023"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..738)
                     /locus_tag="ACHA9N_001035"
     CDS             complement(25..738)
                     /locus_tag="ACHA9N_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084510.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            913..2337
                     /locus_tag="ACHA9N_001036"
     CDS             913..2337
                     /locus_tag="ACHA9N_001036"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9084511.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2636..3343
                     /locus_tag="ACHA9N_001037"
     CDS             2636..3343
                     /locus_tag="ACHA9N_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9084512.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3330..3653
                     /locus_tag="ACHA9N_001038"
     CDS             3330..3653
                     /locus_tag="ACHA9N_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9084513.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3742..4779)
                     /locus_tag="ACHA9N_001039"
     CDS             complement(3742..4779)
                     /locus_tag="ACHA9N_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9084514.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5100..6083)
                     /locus_tag="ACHA9N_001040"
     CDS             complement(5100..6083)
                     /locus_tag="ACHA9N_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9084515.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6498..7268)
                     /locus_tag="ACHA9N_001041"
     CDS             complement(6498..7268)
                     /locus_tag="ACHA9N_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084516.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7261..8196)
                     /locus_tag="ACHA9N_001042"
     CDS             complement(7261..8196)
                     /locus_tag="ACHA9N_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084517.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8512..10161)
                     /locus_tag="ACHA9N_001043"
     CDS             complement(8512..10161)
                     /locus_tag="ACHA9N_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9084518.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10278..12881)
                     /locus_tag="ACHA9N_001044"
     CDS             complement(10278..12881)
                     /locus_tag="ACHA9N_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9084519.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12893..13942)
                     /locus_tag="ACHA9N_001045"
     CDS             complement(12893..13942)
                     /locus_tag="ACHA9N_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9084520.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13968..14282)
                     /locus_tag="ACHA9N_001046"
     CDS             complement(13968..14282)
                     /locus_tag="ACHA9N_001046"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM9084521.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14298..14744)
                     /locus_tag="ACHA9N_001047"
     CDS             complement(14298..14744)
                     /locus_tag="ACHA9N_001047"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9084522.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14745..16667)
                     /locus_tag="ACHA9N_001048"
     CDS             complement(14745..16667)
                     /locus_tag="ACHA9N_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9084523.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(16879..17775)
                     /locus_tag="ACHA9N_001049"
     CDS             complement(16879..17775)
                     /locus_tag="ACHA9N_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9084524.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(17768..18448)
                     /locus_tag="ACHA9N_001050"
     CDS             complement(17768..18448)
                     /locus_tag="ACHA9N_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9084525.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(18449..19231)
                     /locus_tag="ACHA9N_001051"
     CDS             complement(18449..19231)
                     /locus_tag="ACHA9N_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9084526.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19224..20141)
                     /locus_tag="ACHA9N_001052"
     CDS             complement(19224..20141)
                     /locus_tag="ACHA9N_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9084527.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20305..20559)
                     /locus_tag="ACHA9N_001053"
     CDS             complement(20305..20559)
                     /locus_tag="ACHA9N_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084528.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            21416..24643
                     /gene="fss3"
                     /locus_tag="ACHA9N_001054"
     CDS             21416..24643
                     /gene="fss3"
                     /locus_tag="ACHA9N_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9084529.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            24756..25070
                     /locus_tag="ACHA9N_001055"
     CDS             24756..25070
                     /locus_tag="ACHA9N_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9084530.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            25083..25457
                     /locus_tag="ACHA9N_001056"
     CDS             25083..25457
                     /locus_tag="ACHA9N_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9084531.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            25467..25802
                     /locus_tag="ACHA9N_001057"
     CDS             25467..25802
                     /locus_tag="ACHA9N_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9084532.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            25885..27225
                     /locus_tag="ACHA9N_001058"
     CDS             25885..27225
                     /locus_tag="ACHA9N_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9084533.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            27303..27977
                     /locus_tag="ACHA9N_001059"
     CDS             27303..27977
                     /locus_tag="ACHA9N_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9084534.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            27970..28134
                     /locus_tag="ACHA9N_001060"
     CDS             27970..28134
                     /locus_tag="ACHA9N_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084535.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            28210..29352
                     /gene="dcm"
                     /locus_tag="ACHA9N_001061"
                     /pseudo
     CDS             28210..29352
                     /gene="dcm"
                     /locus_tag="ACHA9N_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            29342..29632
                     /locus_tag="ACHA9N_001062"
     CDS             29342..29632
                     /locus_tag="ACHA9N_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084536.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            29891..31075
                     /gene="mobT"
                     /locus_tag="ACHA9N_001063"
     CDS             29891..31075
                     /gene="mobT"
                     /locus_tag="ACHA9N_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9084537.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            31072..31209
                     /locus_tag="ACHA9N_001064"
     CDS             31072..31209
                     /locus_tag="ACHA9N_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9084538.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         9600 a         6053 c         5785 g         9795 t
ORIGIN      
        1 tctgaaaaaa taattttccc gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc
       61 ttgatataaa tgtctcgata ccgaatactc gaaaggttta ccagacgata gagcaacaac
      121 tttgtctatc tcaatcataa attcacctgg atctattttt aatatttttg cgtcaaattc
      181 attcgcagta tctcctttga tacgtatgtg cgaagtcttt atttccagac ccagttcatt
      241 tgtgatataa gcatatacag atttctttaa atgttttggt tccaagcctg gtatgactgc
      301 taatggcatg aatgtttgct caatcgagta tggttgacaa tcaataaaac ggcttcgtac
      361 aatattatat acatagtctt ttacactgat acttaattta tcagctactt ttttcgatgg
      421 aagttcaatt gagaaagtat ggatttctga atctacgtgt tcttcaccaa ccacgctaaa
      481 caaaccttcg tttggaccaa gaactcgtga gttatcaatt ggtgttctga cgacttcagt
      541 cccataccca cttctacttc gcaaaactcc ttcggaaaca agtaagtcta cgcctttctt
      601 tactgttaac ttgcttgtat gataaataac tgccaattca tctcctgtag gaagtttctc
      661 accaatttta tactctccat ttacaatttt tttctttata tctaagtaaa tttccatata
      721 cttcgcaatt tttttcaatg gtgaccctcc aatcaaaaac atatcttcat ctatacttta
      781 acataaataa accgataaaa tccacatcaa aattatacac acaataaaaa agatatatat
      841 atttaaacaa aactatttac tttaagttgt aagcggtgtt atcattaagt ggaaacagga
      901 ggtaaaaaaa ttatgacaac tatgaaaaag ctaacttttc cagatgattt ttggtggggt
      961 tccgcttggt cagctgagca agcagaagga aaaggagaaa caggaaaagc agagactatc
     1021 tgggatcgct ggtttaaaga acaaccttac cggttttatc aacgaatcga ttcctctatt
     1081 acaaccgatc acatccatca atataaagaa gatatagaac taatgaaaaa aactgggcat
     1141 aactcctttc gcgtttctat ttcttgggca cgaatgtttc caaatggcgg taccggaaaa
     1201 gtaaatccca aagccatttc tttctatcga aaattatttt ccgaaatgaa taataacgga
     1261 atcaaagtat ttgccaactt atatcatttt gacatgcctg ctactcttca agataaagga
     1321 ggatgggaat cacgtgaagt agttgatgct tatgttcatt acgctgaaac ttgttttaaa
     1381 gaatttggtg acttagtttg tcattggttt accttcaatg aaccacttgg gcctatttta
     1441 ggtacttatc ttgaagattt tcattatcca aacctcattg actttaaacg cggagcgcaa
     1501 gcagcattct tcactatact tgcacacgca aaagcaatac aagtatttaa agaactagct
     1561 cttcctagta aaataggcgt tattctaaat ttgagtccta cttttcctcg tagtcaaaat
     1621 cctgctgatg tgaaagcagc agaaatcgct gatttatttt atacgcggag ctttttagat
     1681 cctatggtta aagggaattt ttctaaaaag cttatcctgt tattacaaga atataatcag
     1741 cttcctgaag attatacgaa agaagatctt gtatgtatct acgaaaatac cgctcaaatt
     1801 cttggactaa actattacga gccacgtcgc gtcaaatctc gttcttctat ggtggataca
     1861 tctagtccat ttttaccaga gtggtttttc gaaccacata tcatgcctgg acgacgaatg
     1921 aacgaatacc ggggatggga aatttacgaa aaaggaattt atgatttgtg catggatatc
     1981 aagaaccatt atggaaacat cgaagcattt atctcggaga acggaatggg cgttgcggat
     2041 gaagaacgtt tcatggatga tacagggcaa ataattgatg attatcggat tgagtatatc
     2101 aaagatcatc ttgcttactt atggaaggca ataaatgaag gatgtaacat taaggggtat
     2161 catctttgga catttattga ttgctggtca tggatcaacg cctataaaaa ccgatatggg
     2221 ctggtttcac tcgaccttac tactcaaaaa agaacaatta aaaaaagtgg cgaattttac
     2281 aagcaattaa gtgatacaaa tggatttaga tacgatgtag acttgttagt tgaataatat
     2341 tctaaaattc cacttgaagt gaacacatta gaaaacaaga ataatgagaa ataccaataa
     2401 caaatacttt ttcttcatac ttcatttata cttgtcagta taagagactg tgacaaatgt
     2461 agtgatcctc agaagttagg tctggaaatc taacttgaga agattttttt aaactacgtc
     2521 cagtcttttt tatttgttta aaattgaagt tgacaagtct tcattaaaaa tttagaatat
     2581 tcacaagttt gacgacaatt atgaaaattt atgtcgtgct aaaggagttt tgttattgga
     2641 tgaaaagtta gatataaaaa ctgcgatcaa agacacgtta cctaccgttt ttggttatat
     2701 cggtattgga cttgcatttg gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt
     2761 cacgttgatg tccctgctga tctatgctgg ttctgcccaa tttatcacgg tcagcatgct
     2821 tgctagccat agtccactgc tctccatcgt tctctcgacc tttctagtca attcccgaat
     2881 gattctgatg agtatgacga ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct
     2941 gctgattggc acattgctga cagatgaaag tttcgcactg ggaatgaata aattgaacta
     3001 tactggacaa aagttaaact ttcgttggat gaatacagct aattggattt cttatttgac
     3061 gtgggtgggt tcttctcttg ttggcgcact tttagggaat tttattactg atccgaaaaa
     3121 gtttggtttg gatttcgcca tcgtcgctat gtttatcggg ttactttatc ttcaagtcat
     3181 ttctgataga aacacctcta aacgattaca gttgatcctg attggtctca ctttgattct
     3241 ggtatatgtc ggactgattt ttatcccaag taacttagtg attttagttg taacactgat
     3301 tgggtgcgga ttgggggtgt ggatcaaaca tgccttcttt tgagtttaca ttgctgacta
     3361 ttttaggttg tactttagca acttggcttt ctaggatcct accatttgtc ttactgaaaa
     3421 aattcgattt gccgcaaccg ttgcttgagt atttaagttt tgtcccgatc gtcattatgt
     3481 ctgctttatg gttcagcagt ctatttaccc aaaatatcgg acatctgccg cagatcaatc
     3541 tcgaaaatgc tttggcttca gttcctacac ttttagctgc tattctttca aaaagcttat
     3601 tggtgatcgt cttggtagga attctttccc tttcgttgat tcgcttatta taaataaaaa
     3661 aacaagagct tatggcaaat tactgtcatg cgctcttgtt tttatcaaag tcaaataaga
     3721 aaaaaatcgg cgtattcttg cttatccgtt gaacggacca ctaacaacga cttttcctaa
     3781 tatatgtcct gtttctacgt ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc
     3841 tgaatagacc ttgctcaatg tactgcggat aatcccttca tttgctaaag cagcaatgtg
     3901 cgctaaagct tctccttgcg tttcgatttc atagttatag tctgttttag cgaacatata
     3961 ctcccagtcg aagctgatag attggttttt ccaaacacct atatctaacg gctctttgat
     4021 ccctacgatc gttcccacat gtccaaatgg tcggacaagg tgtttgattt gatcaaagta
     4081 gcgggtaata tcgaataata cagcgacata ctctacttga tcgattccta attcacgaaa
     4141 acttttactt aagtcgtgat gataatcgat tggatagtct acacctgttt ttttcagcca
     4201 ttcaaaattc ttcggacttg cagtcgcgta gacttctagg ccagcccaat gcgctagttg
     4261 attcaaaatc gaaccgacgc ccccagctcc gttgatcact aagatttttt tcccgcggtt
     4321 ggcattttct tctggagtca gaccaaattt ttcaaataac aattcataag cggtcaatga
     4381 ggtcaacggc aaggctgcgg cttcttcatc tgaaagattt tttggtgcaa gagcgacaat
     4441 acgttcgtca accagttggt attcttgatt gcttcctgct cttgtcgttg tacctgcgta
     4501 aaaaacgcga tcgccgacag aaaatttttg cacttcatca cctacagcaa caactttccc
     4561 gactccatca aaccctaaga tacgtaaagc atttcttatc ccgttatctt ggcgaagttt
     4621 cgtatcgaca ggattaacag atactgcgtt gacttttacc agaagatccc gtgaactggg
     4681 tacaggtatc tgttcttttt catcaataaa gctttttgga tcatcgatcg acaacccttc
     4741 ataaaaaccg actaccttca ttaattcctt ttctgacata cgaattcctc cttagcttca
     4801 tcaagttgga gcatccatac agttttcatt tcaatatata tagtatgcta tttgttccac
     4861 ttttattcat cataattatt ctcttactta aagtaaacta tttgcacttt tttggttaaa
     4921 aaagaggttg cgacaagact tttgtcacaa cctcttttaa acggtgttca atcaactgac
     4981 cctcggtatt cgctcaaaaa acgacaaaat atgaggagct atttttgttt ggctgttctt
     5041 attacttgat gcaggacagc tttttcacga cctctaattc acggtgttca agcaagaaat
     5101 cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tgtctaagat ctctcgtact
     5161 tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc tggaaatcct
     5221 ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat gatttctgaa
     5281 taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt ttctgtatgt
     5341 tccctcggtt ctttttcaaa tgcataagga ttcttgaaaa tcccccggcc aatcatgatg
     5401 ccatctacgc catatttttc ggccaattct tctcctgtct ggcgatctgg gatatctccg
     5461 ttgatcgtga tcagtgtttg cggagcgatt tggtcacgta aagcaatgat tttagggatg
     5521 acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg gacagaaaga
     5581 ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga catttccgta
     5641 tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagccgc ttcaatcaat
     5701 tgtgccgcta cttctgggcg aaggatcaag ccactgcctt ttccacgttc tgctacatta
     5761 gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc catagcaatg
     5821 ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat cggctgttca
     5881 tcttctgtaa aggtcaatct tcctcgtaca ctgtcttttc catctggatg acagaaactg
     5941 tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt tacgacatgg
     6001 cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc
     6061 tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag cagtgaagtc
     6121 gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag caaagtgaac
     6181 agcttaaaag atgtttctga ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt
     6241 ccttttccgg aataaatggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac
     6301 gaaaaatagg aggagctatt tttgtttggc tgttcttatt acttgatgca gaacagcttt
     6361 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa
     6421 cgacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg
     6481 atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta
     6541 acggcgtaca aaacaacgat gaatagcaca agtacgatca tcgatgttgc tactggcaca
     6601 tcgattgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact
     6661 ttcgaaacag cttgccaaaa tggcggcaac atagagatgg catagaaaac gccacctaga
     6721 taagttaacg gctgcaagac aaatgtaggc acgatcgata catcgtcata agactgagca
     6781 aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg
     6841 atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg
     6901 agagagccga cgagaatact gcgacccact ccgccaatca caaagcccca aatgatcaca
     6961 tgcgtaggaa caggcgctac caagatttct tcgatgtttt tctggaattt ttgagagaag
     7021 aaggacgaag aaacgttggc atacgaacta gtgatagcag acatcataat caaaccaggc
     7081 acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgaccacc gatcattttt
     7141 ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag
     7201 atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga
     7261 ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaaa acagttcttc
     7321 taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg tcagctgatc
     7381 gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct gttcatcttc
     7441 aaatgtacaa gcgtatcctt caatcacagg tttggtctca tatggcgcta aatcaaagat
     7501 aaatgtttca aattgcagtt tggatagaag ctctttcata ctagtattct cgatcagttc
     7561 acctgattgg atgatcccga tgttgcgaca aagcatttca gcttcttcta aataatgcgt
     7621 cgtcaaaatg atcgttgtac cattttcgtt caattctcgg agaaactccc acatctcgcg
     7681 tctcaattca atatcgactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg
     7741 catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg
     7801 gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatcgc
     7861 ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct ggaccgtttc
     7921 aaagggattg aaattgaact cttgcgggac taatccaatc tgttgctttg ctcggactag
     7981 atccgtatcc agatcataac cgaagacatt cacttttcca gatgttttat tgactaaaga
     8041 agtaatgatt ccgatcgttg tagacttccc agctccattt ggtcctaata gcgcatagaa
     8101 atctccttct ttcacggaaa gatcgattcc tcgcaatgct tcgaccccac ttccataaac
     8161 ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc cttatagaca
     8221 tgattcttta ttgtatcatg gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa
     8281 atcaggttgc tgggctcagg aaaatctcct aatgaaagac atcaatcgga cgaagtcaga
     8341 caaccttttc agaaaaaaca aatcgaagat aaaggattac tattcatatg tgcaacaaaa
     8401 ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     8461 ggaagcaaaa gccaagattt ttgatccaat aaatactttc gatcacttcg cctatcccaa
     8521 tacatgccac tgccgctttt catcaaataa cgcggcttcc gtttttgcgg tttctactaa
     8581 agtctctggg taatccaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc
     8641 ttcttttaat ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg
     8701 gacattctca caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa
     8761 tgctaaagat ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc
     8821 gatcgtattt gtccctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc
     8881 cacttggtcc aacaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc
     8941 aaataaatca tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg
     9001 ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac
     9061 atacgtagat tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac
     9121 cggattcact accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc
     9181 ctcagtaaac tctggtcggc aagtgatggg catataagta cggtaattca atattgcagc
     9241 ggctacttct aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc
     9301 taatttagtg agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata
     9361 ttcaaaaatc aactctgctt cactcccact cttcatacga aatgaaaaac ctaatttagg
     9421 gatgtctctt aatggtttag tttgctgttt cagctcttct tgaagtggtg tcggcagttt
     9481 tgctagctga ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt
     9541 ttctagtgtt tgtttcttca ctagataata gaccgtattg aatactaacc caccgattgc
     9601 caaatagatt cctagtactt gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa
     9661 gccaagaaaa ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata
     9721 ttgttttgca aaattatgat ctttttttcc taaacgagcg aattgatatt ggattttttc
     9781 tctcagttga ggattttcct gataaaaagc aatcagtttt tctaactgct gatcttcacc
     9841 aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa cctacactag aataggtact
     9901 gttgattcct tcgaatactt cgaacatatc caggtcatac caagtaagat catcgatttc
     9961 gctatcccat gaacggaact tcttctccgt gagccatgct tccttcaagc tttcttcttt
    10021 atcaaatctc ggttgataag gaattttttc ccatttgcta cgaactgttt cctttaattt
    10081 caaacgtgaa aaaatgtcta aagcaactaa taaaataaca gctgcaataa taattaaaac
    10141 gataaattct ctttgaccca tatccctctc cgttccttta tttgttagct ttagtatagc
    10201 actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt
    10261 tcttgtatat tacatattta tctctcattt atcaaaattg acaaataatc atagggagga
    10321 attgttgtta attcttgttt attctccagt gcattgacaa ctgtcccaag ggaaaggata
    10381 gcctcaggta ctattttttc ttgttctgtt ggattagcta agcgaagaac aaatccttgt
    10441 tcatgaaaat aagaaggata aatcgatgat acaattaagt cttcaggtaa ttcaatcaat
    10501 gaaaatttac gaggaacatc aagttcattt tgtaatggcc aaatcttgtt atctaacctg
    10561 tgaataaata cattcagttt ctgcttttga taagaaatag attgttctaa ccgtttttct
    10621 gcctgttttg ccactttgaa ctcattaaat cttccttctt ctacattaaa tccaaaagtg
    10681 actttatact cacctatctc ttgagctagc ggtgtttcca tcatgacatg cccttcattt
    10741 gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat taggttttcc aagttcccct
    10801 gttgttgaaa ataaggttat gattaattga ttttctatac gttcatactc ttttaaacca
    10861 tctgcataaa acgtaaaata atgatcattt tctgccacag agacactctt atcgaatatt
    10921 tctaaattaa caggtttctc taccatagtt tctgaccaat tcggaggaat gacatcaggg
    10981 gtattttcaa taaatccgtt ttgaatttgt gcaattgatt tttcagattt atctaaaaca
    11041 ttaattgcta agcgcatacg atgcgagtaa acagtattat gaattttgag tgtgccttca
    11101 acgatttgac tttctcccag actgaacgat atttcataag atatttcttc catttcgggt
    11161 ttttcagata aacgatcttt taaatcttta ggaagttgag cttttccgta aacaactaat
    11221 gtttcttgta aagaatcttt atatacttta gctgtttcta attttagaga tagttctgta
    11281 tctccttcta atggtgaata atcatacgta tcgccatcat ttgctgaatc gatcaaatga
    11341 acgaaatcaa gatattgtct atctccaact atcaaattca attgcccatc tttaaaagaa
    11401 agtttatatt tattgttact tatctcagtt ttgtctatct cgattgctgt ttctaatttt
    11461 tcattatttt tctcaaattc aacaacagtg tatcctagtg ctggcacatt tacatttatt
    11521 tttatgtcaa gttcataata agctggttca tcaaaatatt cgaaacctgt agcaatcatc
    11581 cgataagcat ttttccgttc tggaaaataa acttcttttt caatgatatt atagggacta
    11641 cctttgaagt gtattttctt tgaatcagct actacatgca cgattttcga tccactataa
    11701 ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt ttagctttaa tgcatcagcc
    11761 atccgtttta caataaggtt ttcgattccg tcagctatct cattagcttc tttgattcga
    11821 tggaaaatat cttccgcaac attatctgaa acacagccac caatactatc atgagcctgg
    11881 ttttgcaaaa tctttttcca taaccgacga agtaggcctt tactgatatg gataccattt
    11941 ctttctgcaa tcagcatgag cggctcaact cgttttagaa ttttttgttc caatttaaaa
    12001 ttttctattt tcatttttgt tcttactgag ccaatagaac gatgaacacg agagtaaacc
    12061 ggctgacgaa gttcaccttt atattcaggt aattcttttt tgtttctgat aacttctaca
    12121 taagaaggat aatctgaaat aatattgtgg aatttactgt atttattcaa cttctctacg
    12181 acttgatcaa aatttaaaat cattgacttt tgatctacac ccgaaggtac taaaatatct
    12241 ttgttttttc cttgcatatg aagcatttca actgcttcat ccaacttttc ttttgcaaat
    12301 tcaggaatat ctttaattgc gtctaaggtc atcattccag tcatataacc aaatggaaaa
    12361 ttcatggcat aaacaaattt atctcccaat cctttccaga taaaataagg agatggaacc
    12421 atcgtttcaa agtttaatcc tcgccaacac atgaaattat cgatttctac ttgatgcaaa
    12481 agtgtaggca aattcgcatt gaacccaaac gtgtcaggca aataccctac catcattgga
    12541 tctccatatt taaggatcgt atcactgatt ccaatcatta agtttcttaa aattgattca
    12601 gcatccacta ataaggcatc tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt
    12661 ttcatcagtc gttggataac cttaagcttt tgcggattga tctccaaata ctcatcgacg
    12721 attgaagttt gtccatctaa acaaaagttt actttagggt tcctttccaa ttcatctaga
    12781 acttcagtaa agacctgatc agacaatacg atggcatctt catttgtaaa ataccattct
    12841 ctgtcccagt gcgtgtgttg aattatatgt gcatttacca tatcagattc tcctactttc
    12901 cttcttctac tgaatttact tgttgttctt ttttatactt gatggatttt agtgcattga
    12961 ccattaacgc tgtaaccact cccccaataa caattgatac taaatataaa ggtatattcg
    13021 taacaaaagg aattgccata aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc
    13081 catttagacc acatgctact gcagtgccca ccacgatgct tggtattact cgtaaaggat
    13141 ctttcacggc aaacgggatc gtcccttcag taatccccac aaggcccata atcatagctg
    13201 tctttgcatt atctcgttcc gattcatcat aaagtttttt gtttaaaatt gttgctaaaa
    13261 acattccaaa tggcggaata gcaattccta ataacatggc tgtactaggt ccaaagcttc
    13321 cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt attgataggt cctcccatat
    13381 caaacgccat cattcctcct aaaatagccg acatcaaagc agcatttgct cctgacatgc
    13441 cttctagcca attagtcagt cctgttgtca ttgcttgaat aggcgtagct agtacatacc
    13501 ataatatgat tccaacaact aatgtagtaa caaaaggaat cagcataatt gattttagac
    13561 taataacttg catcggtact tttacttttt tcaatgcatt agctaaatag ccacaagcta
    13621 atccagcaac cattcctcca ataaatccca taccaagatt ttgacacatc atcccagtga
    13681 taaaagctgg tgctagaccg ggacggtcag caattgaata tgcgatatag gctgaaatca
    13741 tcggtacgat aaactgtaat cccgtctgac caatcattcg caggactccc cagaatgtat
    13801 ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc aatagaagtt agtaaccctg
    13861 ctgctacgac aagcgggatc atataggaaa ccccactttg gaaatgattt agaatttctc
    13921 ttccttgttt cttgaattcc attagtaact cccccacgat atatttttta tttgttaatt
    13981 aatgcaattg cttcatcaat cgtctcttca ccatctttta ctacctctga ggtccctact
    14041 gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa catttgtatc tactgcaaat
    14101 atgacaacat ctgcctcgga aacatccgaa attgaaagtt tgttttctat ccccatagcc
    14161 ccctgtgttt ctactttaat atctaaccct ttcttcttcg caaacttttc taaattagct
    14221 tttgccatat aagtatgggc aatccctgcg atacatgctg ttactgctac tatttttgtc
    14281 atatgaaatt cctcctttta aaatgataga gcttttacca attctttttt atttctcgaa
    14341 tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc cagataattc tgctaaaata
    14401 cgtaaatgat tagtaccttt atcagtgtct tttattgcta acaaaaagac gagatcaact
    14461 ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta acttggctac agccactgtt
    14521 gattcaatta ctgctaggct tttgccatga ggaatggcga tatttttccc aattcctgtg
    14581 gtcatatggt tttctctgtc aaatacgtct ttttgaaatt gtttgtggtc agacacaaac
    14641 ccattagtct ctaacaattt cgataatttt tcgactacac tttttttatc agatgcattt
    14701 aaatctatca ctatattatt tatattgatt ttttcactga tcatttataa gccttctttc
    14761 aatatataaa ctaatttttc aaacgacgaa ctcgttttta aatttgctaa taactcatga
    14821 ttcgaagcga tttcattgaa tgtttcataa attttattca attcaggttt tactttttca
    14881 ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat cccaatcaat tccttctgga
    14941 ttaacgtaga cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca
    15001 aaatcattca tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct
    15061 tctttcgcgt aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga
    15121 ttatccttta tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca
    15181 tcttctgttt gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac
    15241 atcaaaggac taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata
    15301 acaagttcat cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc
    15361 tcaaccatat ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat
    15421 ccgctactac aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat
    15481 tgaatatgga gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct
    15541 tcgatttcct ctaataaatc tacacttatc ataaatgacc ttttaaaaga atatttgatt
    15601 ttgtccgtat aagggttata gatatttact cccaatttga gtcttttaac tgctgcattg
    15661 agatgtaata ctagattatt gatcaattct tcatcaggat aaaattcatt taatttaatt
    15721 cttactaact cttgtagctc aacacccata tctaaatagg aaagcttcgt ttgatctaag
    15781 tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca
    15841 tctattggca caataattga aaatgctttt tcaatcattt tgattaaaat aagtgtattc
    15901 tttgagattt cttcttcaga attattacta cgtgataaga aattgcttgt ttcaatcctt
    15961 tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt cataatctgt aaatttcaat
    16021 tgtgtcttat tcatgtatcg attaagcaat tcggctactt ttttcaatat gtccttattt
    16081 aagaaattat tcgcttcacc aatgaaattg atattcaagt ctaagagatc tgttttactg
    16141 ttagatgctg taaagccacc ccaataatag ttcacaactt ctgacatcaa ccttctttta
    16201 cttttttcat cagctttgat gcgcacgcca tttcgatctg aaactaagga catcccatgc
    16261 ttgtttagtc tttttctaac atctttcatt gtgctttcta ccgtcgacct gctcacgtat
    16321 aattcgtcag caagattttg aatctttaga gaatcattag tattcaataa tttagcaaac
    16381 acataagtta gacggtcctc cggagagtct gcgatattga ttttatgact ggataataaa
    16441 tttcgaagtg tttctttttt tccaataagc gaaacgcctt ttttaggttg aaccaacatt
    16501 tgtacatctg gcattttttg gattatttct ttcaaaatta aaatatcttt gctaattgtt
    16561 ttcgtagata catataattg ttctgataat tctttgtaag ttgtgtgctc attttcaatc
    16621 aaaaagtata gtattttcat ttgccgttcg ctaaattcga tgcccattat cttcacctcc
    16681 tgaatacagc ttaagtgtac aattattatc tctattttca tacagaagaa agttcttgat
    16741 taatgtagaa gtttctaata ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt
    16801 tatatcacag gcttttttct ctattaaata tatataagat aatacatttt tctactagtt
    16861 ctcactaatc ttgtataatt aaaccctccg caacttgacg gagatggaga agatgccccc
    16921 gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag
    16981 cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa
    17041 gactttttca acagaggttt gcgttcctgg tttgatagga ttggccactg ttaatcgaat
    17101 gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt
    17161 ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt
    17221 tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga
    17281 gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg
    17341 cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac
    17401 agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag
    17461 taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac
    17521 gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc
    17581 atccaaccat gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac
    17641 tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct
    17701 cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa
    17761 cagccattta atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg
    17821 cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa
    17881 aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat
    17941 gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat
    18001 tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg
    18061 acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa
    18121 gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa
    18181 ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc
    18241 ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca
    18301 aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa
    18361 gtttgtatct ctgttcaaga acctcctcca gtaaggaatg aacagcttca tcgtcttcga
    18421 taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca
    18481 ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt
    18541 gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact
    18601 ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaatgaag gccgttccca
    18661 tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag
    18721 ccactggaac agataatgtc cctaatgtca gcagaagcaa tacaggaaat acttttagaa
    18781 cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag
    18841 caagcatggc tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga
    18901 gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg
    18961 taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa
    19021 tccatgcagc cattttttct accattaaaa tccctgttgc accgttgatg actgtatccg
    19081 ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa
    19141 aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt
    19201 ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa
    19261 taatcttcca gtttttgccc aactaggtga ataccatcca caggaacttt tgcttctacc
    19321 aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta
    19381 gattgtacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc
    19441 gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat
    19501 tctttgatca agtgtccttc atgcaaaata ccatagtgag tggcgatctg tgacaattca
    19561 tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg
    19621 atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc
    19681 aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa
    19741 gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct
    19801 tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca
    19861 gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc
    19921 tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata
    19981 atagttctca taaaggtaga cttacccgct ccattttccc caatcaatcc atagatcatt
    20041 ccacgtttga tttcgattga tactttatct aatgctttgg cagcaccgta ttttttggtt
    20101 ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat
    20161 tgatgagtga agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt
    20221 gtaaattttc caattgaatg tcaaaaaaaa caatcggaca atacgtgtct ttcctattat
    20281 caggcagttt gttatacgct tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt
    20341 ttctctgtat tcctcttcac tgaaaatttt tgcttctttc agatcaagct tgagtaattt
    20401 taccagaccg gttataagga taactatagc aagactccct cccagaaaaa ttagactgtc
    20461 tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg ctaatccaag
    20521 tggactggcc accatggtaa tcgttgttat cgttgacata agtcgtcctt gtatatgcgt
    20581 tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa
    20641 acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag
    20701 gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac
    20761 tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc
    20821 ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt
    20881 gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat
    20941 cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc
    21001 agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta
    21061 actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc
    21121 tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaaac cttgatttat
    21181 aaaggttttc ccgtaaatat attttattat atttgattaa atttgaaatg tttgtgttct
    21241 aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt agattaaatt tttaggctca
    21301 ttcattctca aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa
    21361 gatgttcaag cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta
    21421 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat
    21481 tgttggagca attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt
    21541 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
    21601 tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat
    21661 tttctgtatt gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa
    21721 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
    21781 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
    21841 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
    21901 aaaaataaat caagccattg acgattatca gaagaaacca accttccata atagtacggc
    21961 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
    22021 gttcgatgag gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt
    22081 gataatcacc ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc
    22141 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
    22201 gattgataaa cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa
    22261 gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga
    22321 ctttggaaaa actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt
    22381 ggatgagatt cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta
    22441 tacgattgat accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac
    22501 ttcaaaaaac gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg
    22561 gagtgatctt tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga
    22621 cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac
    22681 accaaaagag attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc
    22741 tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca
    22801 aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac
    22861 cactttgacg aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga
    22921 gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc
    22981 ttttaaacca gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt
    23041 agacgaaaag aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga
    23101 aacaaaagcg cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa
    23161 ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt
    23221 gattcgcttt ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac
    23281 tggcttcaat gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg
    23341 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
    23401 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
    23461 tcaaaagatt acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt
    23521 gaagagtgag tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt
    23581 gaccgttcct tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat
    23641 gctctatgat ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg
    23701 aaacaaagaa ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct
    23761 acatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa
    23821 agctgcccaa gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac
    23881 gttggcgaac aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca
    23941 agttttaaat aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga
    24001 agcaggcgat aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag
    24061 ttgtacagtg gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca
    24121 gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt
    24181 actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac
    24241 aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac
    24301 caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac
    24361 ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt
    24421 gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa
    24481 acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac
    24541 cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat
    24601 ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca
    24661 agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt
    24721 gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag
    24781 atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag
    24841 gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac
    24901 gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag
    24961 accaaccgct aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    25021 gtgggtttac tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga
    25081 agatgagatt aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt
    25141 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg
    25201 taaaagaacg aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta
    25261 tcccagctaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc
    25321 caatcgtgga taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca
    25381 ttaaggcgga tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa
    25441 atgaaagaaa gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc
    25501 tcgatcaata tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg
    25561 atgaaaaaat gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag
    25621 aagactctga tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag
    25681 taaatgaaaa cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc
    25741 cggatgctat tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt
    25801 agcagtaaat gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt
    25861 cgattggagg tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag
    25921 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    25981 gtcttttatt ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt
    26041 gaaatcgttt ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga
    26101 gctatgatat tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga
    26161 caaaagctag ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt
    26221 gaaggctttt tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc
    26281 aaaatctgtt atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg
    26341 aaataccaag atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag
    26401 ttagtagaaa aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc
    26461 gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa
    26521 aatcaagtat gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga
    26581 gggaagacct atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg
    26641 tttatcctcg atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt
    26701 tattcacaaa aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct
    26761 cgtagcaagg caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt
    26821 ggacttccac ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg
    26881 acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa
    26941 tctggtttct ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga
    27001 atccgcgatc aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc
    27061 atgatgtttg gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat
    27121 gtggatacag gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
    27181 gatttcttag attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact
    27241 gactttatac ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta
    27301 atatggaaag taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt
    27361 cttatattat caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga
    27421 gtcctggggc tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg
    27481 tgattattga tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta
    27541 gtctctttca aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc
    27601 atggctattc ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct
    27661 cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa
    27721 tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg
    27781 catttattaa cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc
    27841 cagatgtatt gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt
    27901 atgctacgga taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg
    27961 ttcaattaca tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc
    28021 attgcggatt tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta
    28081 gccgatcagg ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac
    28141 aaattaataa tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg
    28201 aggtaatgta tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg
    28261 gaacaggcga gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt
    28321 tataaagcaa ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    28381 gatgagttta ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa
    28441 gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt
    28501 gaaatcgctc ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga
    28561 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    28621 ttggggtatg acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg
    28681 gagcggatat tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    28741 attggaccgt cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc
    28801 aatcgaggaa tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt
    28861 gatgaaggaa tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta
    28921 ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg
    28981 tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag
    29041 gtgaagccag aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt
    29101 ctgggaaaac agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat
    29161 cagtatcaaa ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat
    29221 tgggcttttg atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga
    29281 aactctgtaa cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta
    29341 catggtttat gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga
    29401 cccagaatta gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa
    29461 agtcgctggc gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca
    29521 agtaattgca gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata
    29581 cttgttagaa atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc
    29641 aacacaactg actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg
    29701 tgctgtgcct gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca
    29761 ggggggtaca aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa
    29821 gatagtaagt aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg
    29881 aggtgagaac ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga
    29941 atacggcgtt tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa
    30001 tcagatagaa aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca
    30061 actggaaagc tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg
    30121 ctttcccaca acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta
    30181 tatgctccat gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat
    30241 tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg
    30301 ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga
    30361 ggattgtcta aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt
    30421 ggggctattg gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt
    30481 gtttcgtacc tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg
    30541 catgggcaat accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga
    30601 aaaagactat gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa
    30661 tcgttttgag attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa
    30721 aggtcgtagc attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga
    30781 taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat
    30841 tggacggaat cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg
    30901 aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga
    30961 tcgattgaat cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg
    31021 acataaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga
    31081 ggaatcgtca tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt
    31141 gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa
    31201 ggaaagtgac aagatgaatt tcggacaaaa ttt
//
LOCUS       DAYYJC010000024        30244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000024 DAYYJC010000000
VERSION     DAYYJC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.024"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..720
                     /locus_tag="ACHA9N_001065"
     CDS             22..720
                     /locus_tag="ACHA9N_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9084539.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(888..3527)
                     /locus_tag="ACHA9N_001066"
     CDS             complement(888..3527)
                     /locus_tag="ACHA9N_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9084540.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4146..5177)
                     /gene="queA"
                     /locus_tag="ACHA9N_001067"
     CDS             complement(4146..5177)
                     /gene="queA"
                     /locus_tag="ACHA9N_001067"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9084541.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5370..6545
                     /locus_tag="ACHA9N_001068"
     CDS             5370..6545
                     /locus_tag="ACHA9N_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9084542.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            6549..7190
                     /locus_tag="ACHA9N_001069"
     CDS             6549..7190
                     /locus_tag="ACHA9N_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084543.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            7187..8104
                     /locus_tag="ACHA9N_001070"
     CDS             7187..8104
                     /locus_tag="ACHA9N_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9084544.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            8101..8772
                     /locus_tag="ACHA9N_001071"
     CDS             8101..8772
                     /locus_tag="ACHA9N_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084545.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(9313..9801)
                     /locus_tag="ACHA9N_001072"
     CDS             complement(9313..9801)
                     /locus_tag="ACHA9N_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9084546.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(10002..10268)
                     /locus_tag="ACHA9N_001073"
     CDS             complement(10002..10268)
                     /locus_tag="ACHA9N_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9084547.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(10268..10531)
                     /locus_tag="ACHA9N_001074"
     CDS             complement(10268..10531)
                     /locus_tag="ACHA9N_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9084548.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            11052..11192
                     /locus_tag="ACHA9N_001075"
                     /pseudo
     CDS             11052..11192
                     /locus_tag="ACHA9N_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(11334..13157)
                     /locus_tag="ACHA9N_001076"
     CDS             complement(11334..13157)
                     /locus_tag="ACHA9N_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9084549.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(13755..16460)
                     /locus_tag="ACHA9N_001077"
     CDS             complement(13755..16460)
                     /locus_tag="ACHA9N_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9084550.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(16766..17713)
                     /locus_tag="ACHA9N_001078"
     CDS             complement(16766..17713)
                     /locus_tag="ACHA9N_001078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9084551.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            17903..18172
                     /gene="rpsN"
                     /locus_tag="ACHA9N_001079"
     CDS             17903..18172
                     /gene="rpsN"
                     /locus_tag="ACHA9N_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9084552.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(18348..18710)
                     /locus_tag="ACHA9N_001080"
     CDS             complement(18348..18710)
                     /locus_tag="ACHA9N_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9084553.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(18721..19842)
                     /gene="alr"
                     /locus_tag="ACHA9N_001081"
     CDS             complement(18721..19842)
                     /gene="alr"
                     /locus_tag="ACHA9N_001081"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9084554.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(19858..20208)
                     /gene="acpS"
                     /locus_tag="ACHA9N_001082"
     CDS             complement(19858..20208)
                     /gene="acpS"
                     /locus_tag="ACHA9N_001082"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9084555.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(20338..21849)
                     /locus_tag="ACHA9N_001083"
     CDS             complement(20338..21849)
                     /locus_tag="ACHA9N_001083"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9084556.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(22171..23523)
                     /gene="murF"
                     /locus_tag="ACHA9N_001084"
     CDS             complement(22171..23523)
                     /gene="murF"
                     /locus_tag="ACHA9N_001084"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9084557.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(23676..24752)
                     /locus_tag="ACHA9N_001085"
     CDS             complement(23676..24752)
                     /locus_tag="ACHA9N_001085"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9084558.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            24948..26693
                     /locus_tag="ACHA9N_001086"
     CDS             24948..26693
                     /locus_tag="ACHA9N_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9084559.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(26973..27410)
                     /locus_tag="ACHA9N_001087"
     CDS             complement(26973..27410)
                     /locus_tag="ACHA9N_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9084560.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(27394..28767)
                     /locus_tag="ACHA9N_001088"
     CDS             complement(27394..28767)
                     /locus_tag="ACHA9N_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084561.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(29089..29577)
                     /locus_tag="ACHA9N_001089"
     CDS             complement(29089..29577)
                     /locus_tag="ACHA9N_001089"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9084562.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(29599..30201)
                     /locus_tag="ACHA9N_001090"
     CDS             complement(29599..30201)
                     /locus_tag="ACHA9N_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9084563.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         8671 a         6464 c         5620 g         9489 t
ORIGIN      
        1 aaagaaaagg agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag
       61 agtctaaaag gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt
      121 attgctacta ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat
      181 ggatcagcga gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc
      241 ggaattattt ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt
      301 gatattttac gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc
      361 aaaggtttgt ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca
      421 ttatggggtt tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc
      481 tattttattt actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg
      541 atcactgcaa gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt
      601 tcatttatcg gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc
      661 agcccataca tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa
      721 aagagagtgt gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt
      781 aaaagaaaca ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc
      841 tttttgtata gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct
      901 tctgcttccg gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat
      961 aaaatcccta ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc
     1021 gtaaatggca gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc
     1081 aaagctttat tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac
     1141 acatgcagaa tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga
     1201 cttgcgtcta cccatgggct gatagtatcg acataactac ctaaataata accaaacaag
     1261 gttacgatgg tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag
     1321 atcccttcat ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt
     1381 cctaaagcaa atcctggaag tccatcagca actacattga caaacagtag ttgcacagct
     1441 gtaaatggtg ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata
     1501 ttacagctta gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga
     1561 ctgacagcgt ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt
     1621 tgcgccacat ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct
     1681 ggtgcatcat tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat
     1741 ttgacgatcc ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca
     1801 cgcgcatcta attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac
     1861 ggatcattca agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca
     1921 gtgatcatga ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct
     1981 ggacgtggtg gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct
     2041 tcagaagaaa tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc
     2101 agtgcccgtt taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta
     2161 aagcgcggag caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt
     2221 tggtgaactg ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga
     2281 tatttttctt ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct
     2341 acttctgttg gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct
     2401 gtacatagtg cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata
     2461 ttcgttactt catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc
     2521 gttcctgttt tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac
     2581 tgtcggatga tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta
     2641 acaataacag tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac
     2701 atctcaagga gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct
     2761 aaagcgatca tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt
     2821 tgcatagtat tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg
     2881 gcagtgacga tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta
     2941 agttggtcac ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct
     3001 tcactttctc cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg
     3061 gcatcggcag aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc
     3121 tctgactcaa cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata
     3181 tcttgcaagg attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag
     3241 ttgatcacaa cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca
     3301 aatgcggtat aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac
     3361 aagcttcgaa agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat
     3421 ccctgctttt ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct
     3481 tctgttacct tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt
     3541 taaaatcttc gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt
     3601 caaataaagg gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt
     3661 gcaaacgcta ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag
     3721 acagaagaat ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc
     3781 acccagacat gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat
     3841 cacgaagtat ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct
     3901 gaaaattgaa taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt
     3961 ttgtttaaca tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca
     4021 tttctgttta tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct
     4081 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
     4141 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
     4201 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
     4261 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
     4321 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
     4381 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
     4441 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
     4501 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
     4561 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
     4621 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
     4681 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
     4741 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
     4801 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
     4861 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
     4921 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
     4981 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
     5041 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     5101 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     5161 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     5221 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     5281 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     5341 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     5401 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     5461 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     5521 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     5581 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     5641 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     5701 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     5761 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     5821 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     5881 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     5941 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     6001 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     6061 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     6121 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     6181 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     6241 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     6301 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     6361 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     6421 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     6481 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     6541 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     6601 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     6661 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     6721 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     6781 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     6841 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     6901 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     6961 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     7021 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     7081 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     7141 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     7201 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     7261 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     7321 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     7381 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     7441 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     7501 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     7561 aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     7621 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     7681 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     7741 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     7801 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     7861 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     7921 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     7981 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     8041 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
     8101 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
     8161 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
     8221 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
     8281 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
     8341 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
     8401 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
     8461 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
     8521 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
     8581 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
     8641 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
     8701 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
     8761 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
     8821 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
     8881 ggtaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
     8941 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
     9001 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
     9061 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
     9121 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
     9181 ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg
     9241 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
     9301 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
     9361 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
     9421 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
     9481 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
     9541 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
     9601 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
     9661 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
     9721 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
     9781 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
     9841 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
     9901 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
     9961 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
    10021 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
    10081 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
    10141 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
    10201 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
    10261 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
    10321 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
    10381 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
    10441 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
    10501 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
    10561 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
    10621 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
    10681 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
    10741 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
    10801 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
    10861 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
    10921 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
    10981 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
    11041 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
    11101 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
    11161 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
    11221 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
    11281 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
    11341 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
    11401 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
    11461 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
    11521 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
    11581 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
    11641 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
    11701 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
    11761 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
    11821 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
    11881 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
    11941 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
    12001 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
    12061 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
    12121 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
    12181 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
    12241 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
    12301 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
    12361 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
    12421 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
    12481 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
    12541 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
    12601 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
    12661 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
    12721 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
    12781 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
    12841 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
    12901 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
    12961 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
    13021 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
    13081 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
    13141 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
    13201 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
    13261 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
    13321 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
    13381 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
    13441 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
    13501 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
    13561 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
    13621 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
    13681 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
    13741 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
    13801 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
    13861 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
    13921 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
    13981 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
    14041 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    14101 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    14161 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    14221 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    14281 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    14341 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    14401 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    14461 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    14521 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    14581 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    14641 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    14701 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    14761 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    14821 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    14881 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    14941 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    15001 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    15061 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    15121 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    15181 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    15241 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    15301 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    15361 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    15421 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    15481 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    15541 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    15601 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    15661 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    15721 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    15781 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    15841 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    15901 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    15961 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    16021 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    16081 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    16141 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    16201 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    16261 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    16321 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    16381 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    16441 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    16501 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    16561 cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct
    16621 ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt atctattata
    16681 cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt
    16741 ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag
    16801 gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat
    16861 tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg
    16921 cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt
    16981 gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga
    17041 tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct
    17101 gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta
    17161 atttttttac cttttcctca tttttactgg atgcactggt taagattcgg attccctttt
    17221 catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag
    17281 tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg
    17341 tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg
    17401 atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac
    17461 ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac
    17521 ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa
    17581 catcataagg attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt
    17641 ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg
    17701 caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt
    17761 tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt
    17821 tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa
    17881 aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca
    17941 gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta
    18001 cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt
    18061 aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg
    18121 tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc
    18181 tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac
    18241 gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca
    18301 gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca
    18361 ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg
    18421 ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc
    18481 tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga
    18541 gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta
    18601 aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat
    18661 cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc
    18721 ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat
    18781 cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat
    18841 aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat
    18901 acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat
    18961 ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg
    19021 agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat
    19081 caattcagaa accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc
    19141 agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc
    19201 atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt
    19261 tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc
    19321 tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa
    19381 tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc
    19441 tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc
    19501 tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt
    19561 cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct
    19621 caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc
    19681 tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa
    19741 ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt
    19801 atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta
    19861 gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac
    19921 attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc
    19981 gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt
    20041 gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag
    20101 tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg
    20161 agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct
    20221 tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc cacaaataag
    20281 tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta
    20341 atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc
    20401 tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc
    20461 gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt
    20521 tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc
    20581 ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa
    20641 tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg
    20701 ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt
    20761 cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt
    20821 aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac
    20881 gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc
    20941 taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga
    21001 acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta
    21061 accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac
    21121 gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta
    21181 ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat
    21241 cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat
    21301 cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat
    21361 atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac
    21421 ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac
    21481 aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta
    21541 aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg
    21601 gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc
    21661 gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg
    21721 tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc
    21781 ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt
    21841 aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc
    21901 cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta
    21961 tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa
    22021 aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt
    22081 ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata
    22141 gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac
    22201 ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt
    22261 ttgtttgata gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg
    22321 ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat
    22381 catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc
    22441 tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt
    22501 tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa
    22561 aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg
    22621 ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc
    22681 aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc
    22741 tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt
    22801 tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg
    22861 ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt
    22921 ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt
    22981 gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc
    23041 catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg
    23101 aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa
    23161 gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac
    23221 ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc
    23281 tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga
    23341 agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc
    23401 aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat
    23461 ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt
    23521 cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg
    23581 catgactctc acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg
    23641 ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat
    23701 tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat
    23761 ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa tcctggcata
    23821 gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc
    23881 aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa
    23941 acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct
    24001 tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct
    24061 tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct
    24121 cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct
    24181 tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa
    24241 gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact
    24301 ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta
    24361 tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct
    24421 aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg
    24481 gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc
    24541 tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt
    24601 aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa
    24661 ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg
    24721 ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt
    24781 tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa
    24841 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
    24901 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
    24961 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
    25021 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
    25081 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
    25141 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
    25201 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
    25261 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
    25321 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
    25381 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
    25441 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
    25501 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
    25561 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
    25621 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
    25681 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
    25741 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
    25801 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
    25861 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
    25921 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
    25981 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
    26041 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
    26101 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
    26161 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
    26221 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
    26281 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
    26341 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
    26401 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
    26461 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
    26521 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
    26581 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
    26641 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
    26701 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
    26761 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
    26821 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
    26881 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
    26941 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
    27001 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
    27061 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
    27121 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
    27181 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
    27241 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
    27301 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
    27361 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
    27421 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
    27481 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
    27541 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
    27601 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
    27661 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
    27721 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
    27781 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
    27841 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
    27901 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
    27961 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
    28021 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
    28081 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
    28141 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
    28201 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
    28261 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
    28321 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
    28381 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
    28441 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
    28501 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
    28561 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
    28621 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
    28681 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
    28741 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
    28801 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
    28861 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
    28921 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
    28981 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
    29041 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
    29101 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
    29161 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
    29221 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
    29281 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
    29341 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
    29401 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
    29461 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaaacttccg gccatacttc
    29521 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
    29581 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
    29641 catgctatac acacccaccg accacggttt caagaaaatc acaatctgca taaattccct
    29701 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
    29761 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
    29821 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
    29881 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
    29941 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
    30001 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
    30061 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
    30121 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
    30181 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
    30241 tcct
//
LOCUS       DAYYJC010000025        29917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000025 DAYYJC010000000
VERSION     DAYYJC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.025"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHA9N_001091"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ACHA9N_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9084564.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHA9N_001092"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ACHA9N_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9084565.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHA9N_001093"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ACHA9N_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9084566.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ACHA9N_001094"
     CDS             1642..2274
                     /locus_tag="ACHA9N_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9084567.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ACHA9N_001095"
     CDS             complement(2276..3181)
                     /locus_tag="ACHA9N_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9084568.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ACHA9N_001096"
     CDS             complement(3266..3925)
                     /locus_tag="ACHA9N_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9084569.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ACHA9N_001097"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ACHA9N_001097"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9084570.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHA9N_001098"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ACHA9N_001098"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9084571.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ACHA9N_001099"
     CDS             6168..7253
                     /locus_tag="ACHA9N_001099"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9084572.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /gene="fni"
                     /locus_tag="ACHA9N_001100"
     CDS             7250..8314
                     /gene="fni"
                     /locus_tag="ACHA9N_001100"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9084573.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ACHA9N_001101"
     CDS             complement(8563..9366)
                     /locus_tag="ACHA9N_001101"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9084574.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHA9N_001102"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ACHA9N_001102"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9084575.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ACHA9N_001103"
     CDS             complement(10244..11251)
                     /locus_tag="ACHA9N_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9084576.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ACHA9N_001104"
     CDS             complement(11258..11902)
                     /locus_tag="ACHA9N_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9084577.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ACHA9N_001105"
     CDS             complement(12002..12760)
                     /locus_tag="ACHA9N_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9084578.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHA9N_001106"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ACHA9N_001106"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9084579.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHA9N_001107"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ACHA9N_001107"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9084580.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHA9N_001108"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ACHA9N_001108"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9084581.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHA9N_001109"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ACHA9N_001109"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9084582.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHA9N_001110"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ACHA9N_001110"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9084583.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ACHA9N_001111"
     CDS             18158..19027
                     /locus_tag="ACHA9N_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9084584.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ACHA9N_001112"
     CDS             complement(19153..20052)
                     /locus_tag="ACHA9N_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084585.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ACHA9N_001113"
     CDS             complement(20217..21041)
                     /locus_tag="ACHA9N_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9084586.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ACHA9N_001114"
     CDS             complement(21052..22347)
                     /locus_tag="ACHA9N_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9084587.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ACHA9N_001115"
     CDS             complement(22363..22842)
                     /locus_tag="ACHA9N_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9084588.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ACHA9N_001116"
     CDS             complement(22848..23876)
                     /locus_tag="ACHA9N_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9084589.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ACHA9N_001117"
     CDS             complement(23893..24999)
                     /locus_tag="ACHA9N_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9084590.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHA9N_001118"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ACHA9N_001118"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9084591.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHA9N_001119"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ACHA9N_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9084592.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHA9N_001120"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ACHA9N_001120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9084593.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
BASE COUNT         8426 a         6551 c         5363 g         9577 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaat
//
LOCUS       DAYYJC010000026        29290 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000026 DAYYJC010000000
VERSION     DAYYJC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.026"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..881)
                     /locus_tag="ACHA9N_001121"
     CDS             complement(33..881)
                     /locus_tag="ACHA9N_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9084594.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACHA9N_001122"
     CDS             complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACHA9N_001122"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9084595.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3382..3561)
                     /locus_tag="ACHA9N_001123"
     CDS             complement(3382..3561)
                     /locus_tag="ACHA9N_001123"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9084596.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3608..5431)
                     /locus_tag="ACHA9N_001124"
     CDS             complement(3608..5431)
                     /locus_tag="ACHA9N_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9084597.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5709..7988
                     /gene="helD"
                     /locus_tag="ACHA9N_001125"
     CDS             5709..7988
                     /gene="helD"
                     /locus_tag="ACHA9N_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9084598.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8081..8728)
                     /locus_tag="ACHA9N_001126"
     CDS             complement(8081..8728)
                     /locus_tag="ACHA9N_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084599.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8907..9626)
                     /locus_tag="ACHA9N_001127"
     CDS             complement(8907..9626)
                     /locus_tag="ACHA9N_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084600.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9757..10908)
                     /locus_tag="ACHA9N_001128"
     CDS             complement(9757..10908)
                     /locus_tag="ACHA9N_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9084601.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11125..11865)
                     /locus_tag="ACHA9N_001129"
     CDS             complement(11125..11865)
                     /locus_tag="ACHA9N_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9084602.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACHA9N_001130"
     CDS             complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACHA9N_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9084603.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACHA9N_001131"
     CDS             complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACHA9N_001131"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9084604.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12826..13476)
                     /locus_tag="ACHA9N_001132"
     CDS             complement(12826..13476)
                     /locus_tag="ACHA9N_001132"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9084605.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACHA9N_001133"
     CDS             complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACHA9N_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9084606.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACHA9N_001134"
     CDS             complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACHA9N_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9084607.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14919..15446)
                     /locus_tag="ACHA9N_001135"
     CDS             complement(14919..15446)
                     /locus_tag="ACHA9N_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9084608.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15626..16411)
                     /locus_tag="ACHA9N_001136"
     CDS             complement(15626..16411)
                     /locus_tag="ACHA9N_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9084609.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACHA9N_001137"
     CDS             complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACHA9N_001137"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9084610.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17274..18653)
                     /locus_tag="ACHA9N_001138"
     CDS             complement(17274..18653)
                     /locus_tag="ACHA9N_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9084611.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACHA9N_001139"
     CDS             complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACHA9N_001139"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9084612.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACHA9N_001140"
     CDS             complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACHA9N_001140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9084613.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACHA9N_001141"
     CDS             complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACHA9N_001141"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9084614.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACHA9N_001142"
     CDS             complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACHA9N_001142"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9084615.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACHA9N_001143"
     CDS             complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACHA9N_001143"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9084616.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22611..22835)
                     /locus_tag="ACHA9N_001144"
     CDS             complement(22611..22835)
                     /locus_tag="ACHA9N_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9084617.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22888..23850)
                     /locus_tag="ACHA9N_001145"
     CDS             complement(22888..23850)
                     /locus_tag="ACHA9N_001145"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9084618.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23860..24300)
                     /locus_tag="ACHA9N_001146"
     CDS             complement(23860..24300)
                     /locus_tag="ACHA9N_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9084619.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24606..25568
                     /locus_tag="ACHA9N_001147"
     CDS             24606..25568
                     /locus_tag="ACHA9N_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9084620.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKRS"
     gene            complement(25641..25871)
                     /locus_tag="ACHA9N_001148"
     CDS             complement(25641..25871)
                     /locus_tag="ACHA9N_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9084621.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25957..26844)
                     /locus_tag="ACHA9N_001149"
     CDS             complement(25957..26844)
                     /locus_tag="ACHA9N_001149"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9084622.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26933..28156)
                     /locus_tag="ACHA9N_001150"
     CDS             complement(26933..28156)
                     /locus_tag="ACHA9N_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9084623.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28173..29219)
                     /locus_tag="ACHA9N_001151"
     CDS             complement(28173..29219)
                     /locus_tag="ACHA9N_001151"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9084624.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8121 a         6417 c         5050 g         9702 t
ORIGIN      
        1 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
       61 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
      121 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
      181 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
      241 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
      301 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
      361 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
      421 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
      481 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
      541 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
      601 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
      661 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
      721 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
      781 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
      841 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
      901 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
      961 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
     1021 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
     1081 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
     1141 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
     1201 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
     1261 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
     1321 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
     1381 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
     1441 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
     1501 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
     1561 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
     1621 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
     1681 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
     1741 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
     1801 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
     1861 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
     1921 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
     1981 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
     2041 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
     2101 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
     2161 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
     2221 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
     2281 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
     2341 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
     2401 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
     2461 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
     2521 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
     2581 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
     2641 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
     2701 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
     2761 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
     2821 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
     2881 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
     2941 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
     3001 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
     3061 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
     3121 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
     3181 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
     3241 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
     3301 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
     3361 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
     3421 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
     3481 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
     3541 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
     3601 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
     3661 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
     3721 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
     3781 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
     3841 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
     3901 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
     3961 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
     4021 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
     4081 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
     4141 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
     4201 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
     4261 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
     4321 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
     4381 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
     4441 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
     4501 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
     4561 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
     4621 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
     4681 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
     4741 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
     4801 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
     4861 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
     4921 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
     4981 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
     5041 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
     5101 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
     5161 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
     5221 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
     5281 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
     5341 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
     5401 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
     5461 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
     5521 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
     5581 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
     5641 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
     5701 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
     5761 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
     5821 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
     5881 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
     5941 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
     6001 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
     6061 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
     6121 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
     6181 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
     6241 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
     6301 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
     6361 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
     6421 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
     6481 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
     6541 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
     6601 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
     6661 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
     6721 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
     6781 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
     6841 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
     6901 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
     6961 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
     7021 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
     7081 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
     7141 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
     7201 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
     7261 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
     7321 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
     7381 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
     7441 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
     7501 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
     7561 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
     7621 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
     7681 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
     7741 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
     7801 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
     7861 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
     7921 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
     7981 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
     8041 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
     8101 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
     8161 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
     8221 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
     8281 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
     8341 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
     8401 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
     8461 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
     8521 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
     8581 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
     8641 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
     8701 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
     8761 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
     8821 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
     8881 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
     8941 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
     9001 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
     9061 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
     9121 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
     9181 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
     9241 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
     9301 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
     9361 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
     9421 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
     9481 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
     9541 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
     9601 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
     9661 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
     9721 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
     9781 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
     9841 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
     9901 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
     9961 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    10021 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    10081 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    10141 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    10201 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    10261 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    10321 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    10381 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    10441 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    10501 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    10561 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    10621 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    10681 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    10741 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    10801 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    10861 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    10921 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    10981 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    11041 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    11101 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    11161 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    11221 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    11281 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    11341 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    11401 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    11461 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    11521 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    11581 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    11641 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    11701 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    11761 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    11821 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    11881 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    11941 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    12001 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    12061 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    12121 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    12181 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    12241 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    12301 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    12361 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    12421 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    12481 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    12541 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    12601 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    12661 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    12721 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    12781 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    12841 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    12901 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    12961 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    13021 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    13081 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    13141 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    13201 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    13261 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    13321 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    13381 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    13441 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    13501 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    13561 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    13621 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    13681 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    13741 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    13801 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    13861 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    13921 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    13981 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    14041 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    14101 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    14161 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    14221 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    14281 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    14341 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    14401 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    14461 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    14521 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    14581 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    14641 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    14701 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    14761 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    14821 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    14881 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    14941 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    15001 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    15061 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    15121 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    15181 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    15241 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    15301 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    15361 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    15421 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    15481 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    15541 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    15601 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    15661 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    15721 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    15781 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    15841 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    15901 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    15961 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    16021 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    16081 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    16141 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    16201 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    16261 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    16321 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    16381 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    16441 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    16501 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    16561 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    16621 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    16681 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    16741 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    16801 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    16861 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    16921 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    16981 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    17041 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    17101 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    17161 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    17221 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    17281 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    17341 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    17401 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    17461 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    17521 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    17581 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    17641 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    17701 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    17761 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    17821 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    17881 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    17941 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    18001 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    18061 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    18121 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    18181 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    18241 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    18301 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    18361 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    18421 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    18481 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    18541 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    18601 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    18661 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    18721 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    18781 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    18841 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    18901 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    18961 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    19021 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    19081 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    19141 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    19201 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    19261 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    19321 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    19381 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    19441 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    19501 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    19561 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    19621 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    19681 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    19741 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    19801 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    19861 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    19921 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    19981 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    20041 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    20101 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    20161 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    20221 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    20281 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    20341 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    20401 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    20461 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    20521 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    20581 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    20641 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    20701 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    20761 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    20821 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    20881 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    20941 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    21001 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    21061 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    21121 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    21181 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    21241 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    21301 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    21361 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    21421 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    21481 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    21541 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    21601 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    21661 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    21721 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    21781 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    21841 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    21901 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    21961 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    22021 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    22081 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    22141 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    22201 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    22261 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    22321 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    22381 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    22441 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    22501 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    22561 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    22621 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    22681 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    22741 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    22801 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    22861 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    22921 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    22981 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    23041 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    23101 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    23161 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    23221 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    23281 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    23341 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    23401 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    23461 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    23521 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    23581 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    23641 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    23701 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    23761 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    23821 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    23881 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    23941 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    24001 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    24061 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    24121 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    24181 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    24241 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    24301 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    24361 acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga
    24421 ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc
    24481 atctatctca catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc
    24541 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
    24601 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
    24661 aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
    24721 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
    24781 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
    24841 aattgatcca acattaccgt cctttttata accgacaaat gaattatttc agtaattata
    24901 attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat
    24961 acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt
    25021 atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc
    25081 tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa
    25141 ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt
    25201 tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga
    25261 tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag
    25321 cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga
    25381 ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt
    25441 tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca
    25501 aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaaaa
    25561 gaagctgaaa agcaactaaa ttagttgctt ctcggcttct tttaggcatc ttcaacttca
    25621 aatgtcaatt atcctaaaat ttattggatt aagtcataga tttccatcgc tacgatatcg
    25681 atattatcaa attcgtattg ctgatgagta tctgtttcag ttaattcaaa tgtatcggtc
    25741 gctttatcat aagttactgt acacttctct acgccttctt tttcaaaacg gcgaacttgc
    25801 agttcattat ctgctgactc gaccatagcc tccagtcgtt ttatgattgc gactaattgt
    25861 gaatgtgtca tcggaggtcc tccttttctt atttctgtta tattgtatca aaagcatagc
    25921 ctacttgcat tgattttctc taaaaaaact cgttttttat gatttttttt ctggtatttt
    25981 tccttcaggc caccaaagtt tgatcaaagc ttgggttccg tcattctcaa aacctttatc
    26041 tgccaactct tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa gatccatttt
    26101 ttctgcttct tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa aaaaaccagg
    26161 tgtgtaatct tcttttaaaa tacgaggaga ataattgctt aaagaccagt tagctgcact
    26221 tccacctgaa agcgtctcaa tcactttctt taaatcaagt ccgttttttt gtgcatagac
    26281 aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg atctgattag ccatttttgt
    26341 atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt ctttgaataa
    26401 aggcagtact tgatcataaa cggcttcttc tccaccgacc atgatcgtca gtgtaccatt
    26461 ctttgctcct aagtcaccac cggataccgg tgcatctaac gcatctgcgc ctttttcttt
    26521 tgctgttttt gcaatttttt ctgccaggct tggtgtactt gtagtcaaat caactaaaat
    26581 atgtcctgag atatctgcag aaaaaatccc cgactgtcca tagtagattt cttctacatc
    26641 tttggggtaa cctaccattg taaagagaat attactttgt tctgctaccg cttttggtgt
    26701 atctgcccag atagctcctt ctgttactaa aggatcagct tttgcttttg tccgattata
    26761 gacagctact tcgtgtcctg cttctagtag atggcgtgcg acagcacttc ccatgacgcc
    26821 tgttccgata aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa tccttgcttc
    26881 tatgctagta tacgctagaa acaaggattt tttcataaaa aatgtttgaa ttttatcttt
    26941 taaatgaatt taattttatt ttgattcgtt caatcgagtc ttcttcttct tttcttcgca
    27001 tctcttcttc atgataaaca accatgtctt gataaaattc gatcatagct tcgccgaaat
    27061 gtgtagatga aatatcataa agtttagctt ctaaaacttt tggatcttta ggaacttcac
    27121 tggaaatata atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa gttttcccta
    27181 atgaaggatg atcgaacaat cggttcaaat aagcatttcc ttccacgaca caaggtgttc
    27241 cggctgccat cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa gcactgacaa
    27301 aataatctgc cgctttataa taaagtgcta catcattgtt gtccacttct cctgtgaact
    27361 gcacaaattc ttctaactgc agttctgaga tcaattcctc taactcttct acataaggtc
    27421 cttttcctac gataactaat ctggcgttag gataagcgga gatgacctga ggaaacccat
    27481 taatcaaggc ttggatattt ttctcataag aaatcctgct taaagaaaga atcatcaaat
    27541 ggttttcctg aagacctagt gactccctta atttgctgat atcctgctga gtgatatcag
    27601 aacgtttgaa tttttcaatg tcgataccag taggaatcac gcgaagcggt gctattacac
    27661 catagctgcg taatgtatca ataacacgtt cacttggaca aactacacct gtcgtatgat
    27721 ttgcaaataa tctagacaag tattttacat gggattgcct gatcactttt cctttaccga
    27781 tataatgaag gtagtcttca tacatcgtat ggtaagtatg gatcagaggt attttcaact
    27841 tcttagctac catcttccca agtatccctg ctccgaattc tgtatgggta tgaataagat
    27901 ctagttctag ttctttggca accaaataag cataccacat gcctctaaca acaactcttc
    27961 gatccttgaa agaaataaat ggtacacttg gcattcgaat cacatctttt tccaacttat
    28021 ccgcattagg atcggtcgtt gtaaaaatat agacttcatg cccatgtctt tctaattctt
    28081 gttttaatgt tttgatcgat gtagcaactc cgctgacttg cggaaagtag gtatcggtaa
    28141 aaaagccgat tttcatgttt ttcgcctcca aactagtcct tgacctgatt tacttttttt
    28201 aatttgctag agactttttc attcagtaca ctttcgtata catttttaag ttcttcaccg
    28261 attttattga tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt actcggtagt
    28321 tcttgattgg taatctgttg gatcagtcgg ataaattctt cgtttgtctt ccccatatag
    28381 cagttcttgc tatcttctaa ccagccatta tacactggaa tatctcgaac aagtacgttt
    28441 tgtttgcttg ctaaagcttc taaaacaacg atcccttctg tctcttcata agaagggaaa
    28501 aagaatagat ctgcacctga atacgcacct tctatgatct ctccacgaat gtaaccagga
    28561 aacaggacat tttcaggatg atcttctttt acaattttcc ggatgttccg aggaatcgag
    28621 tacatgggaa catgaccaaa ccaaataaat gtgtattcgg gcatcttctt agcaatttct
    28681 acgaaatcaa tgattccttt acgttcgaaa aacaaaccga cacaaatgat tactttttga
    28741 tccggagaca gtttgaaata ttctctgaac ttttgttctt tcattggatc agggtgatat
    28801 ttctctaagt cgatcccatt ggaaatagct tgaatcggca cacggatacc atagctttcc
    28861 aacaactgtt ttgaataagg tgttggcgtg atcagatagt ccgctttttg gtacaatcct
    28921 accagatatt tttttacgat aggagaaagc tggttcgatc cgataaagga atttcggaaa
    28981 tcttcctctg tagaatgcgc atgatagaca actttttttc cttctcttct ggctttattg
    29041 accatattat gactattgat tccgtatgta ttgatgtgca ggatgtcata atcttctttt
    29101 tcatctagtg tataagagat accgacttcc gtcagagcac gtttttgatg atcaagcgca
    29161 cgcccgattc cagattttgc aagcatcttt tcactttcaa aatataataa taccttcacc
    29221 ttacgtcccc cttcacttat atagtataac atagcctaca aactgtcact caaacttaag
    29281 ccctattttt
//
LOCUS       DAYYJC010000027        27881 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000027 DAYYJC010000000
VERSION     DAYYJC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.027"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(419..1363)
                     /locus_tag="ACHA9N_001152"
     CDS             complement(419..1363)
                     /locus_tag="ACHA9N_001152"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9084625.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1659..2603)
                     /locus_tag="ACHA9N_001153"
     CDS             complement(1659..2603)
                     /locus_tag="ACHA9N_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9084626.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2823..4037
                     /locus_tag="ACHA9N_001154"
     CDS             2823..4037
                     /locus_tag="ACHA9N_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9084627.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4059..6074
                     /locus_tag="ACHA9N_001155"
     CDS             4059..6074
                     /locus_tag="ACHA9N_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9084628.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6157..7530)
                     /locus_tag="ACHA9N_001156"
     CDS             complement(6157..7530)
                     /locus_tag="ACHA9N_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9084629.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7556..7840)
                     /locus_tag="ACHA9N_001157"
     CDS             complement(7556..7840)
                     /locus_tag="ACHA9N_001157"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9084630.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7842..9857)
                     /locus_tag="ACHA9N_001158"
     CDS             complement(7842..9857)
                     /locus_tag="ACHA9N_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9084631.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10036..10341)
                     /locus_tag="ACHA9N_001159"
     CDS             complement(10036..10341)
                     /locus_tag="ACHA9N_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9084632.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10430..11461)
                     /locus_tag="ACHA9N_001160"
     CDS             complement(10430..11461)
                     /locus_tag="ACHA9N_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9084633.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11668..12543
                     /locus_tag="ACHA9N_001161"
     CDS             11668..12543
                     /locus_tag="ACHA9N_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9084634.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12695..13537)
                     /locus_tag="ACHA9N_001162"
     CDS             complement(12695..13537)
                     /locus_tag="ACHA9N_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9084635.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13629..15506)
                     /locus_tag="ACHA9N_001163"
     CDS             complement(13629..15506)
                     /locus_tag="ACHA9N_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9084636.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15503..16924)
                     /locus_tag="ACHA9N_001164"
     CDS             complement(15503..16924)
                     /locus_tag="ACHA9N_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9084637.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16927..20316)
                     /locus_tag="ACHA9N_001165"
     CDS             complement(16927..20316)
                     /locus_tag="ACHA9N_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9084638.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20573..21964
                     /locus_tag="ACHA9N_001166"
     CDS             20573..21964
                     /locus_tag="ACHA9N_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084639.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22037..22684)
                     /locus_tag="ACHA9N_001167"
     CDS             complement(22037..22684)
                     /locus_tag="ACHA9N_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9084640.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22692..23378)
                     /locus_tag="ACHA9N_001168"
     CDS             complement(22692..23378)
                     /locus_tag="ACHA9N_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084641.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23384..24457)
                     /locus_tag="ACHA9N_001169"
     CDS             complement(23384..24457)
                     /locus_tag="ACHA9N_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084642.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24679..25353
                     /locus_tag="ACHA9N_001170"
     CDS             24679..25353
                     /locus_tag="ACHA9N_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9084643.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25358..26698
                     /locus_tag="ACHA9N_001171"
     CDS             25358..26698
                     /locus_tag="ACHA9N_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9084644.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26847..27833)
                     /locus_tag="ACHA9N_001172"
     CDS             complement(26847..27833)
                     /locus_tag="ACHA9N_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9084645.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8128 a         5268 c         5053 g         9432 t
ORIGIN      
        1 tgataaacct ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc
       61 ttatgcaaaa ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa
      121 tgataaaaac cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat
      181 tgtatttgcc gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc
      241 gcttattgta atcaacagat taaatttcat atgttcatat gtacggatac attttcggac
      301 cgattaattt taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct
      361 taacagctat ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt
      421 ataatacagt gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt
      481 cttcttcgtt gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg
      541 gcacacctgt aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca
      601 atacagcttg ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac
      661 catagtaagt tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag
      721 caatcacatc taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg
      781 gtttaccacc gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta
      841 agatatagcc gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga
      901 aacgagttgt atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt
      961 cgttccaagc gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt
     1021 cgaatcctga tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa
     1081 ctaaatctaa acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat
     1141 cagcatcttt acattcttca tagccacctg accatacagc cacattttca tcgccccaag
     1201 ccataccatc taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca
     1261 atactaattc attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa
     1321 aacctgtacc gacgattact actttacgac ttgttttttt catgaataaa cagctccttt
     1381 tcataactat ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg
     1441 ttaattataa tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac
     1501 agataaatga caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact
     1561 gaaaataaat ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa
     1621 caattttcct tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca
     1681 ttgggagtac atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat
     1741 ccgatacgat tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca
     1801 aagtcgaatt ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat
     1861 ttattactaa gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa
     1921 gaagtaaaat gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca
     1981 tctgctgatg aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat
     2041 gtatagagat ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata
     2101 ttttcaatca aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta
     2161 tgttcccctt tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt
     2221 gccaaatgat tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa
     2281 atatcctctt gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg
     2341 tcactttctt ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac
     2401 atataattga tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt
     2461 ttactgatat gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc
     2521 tgaagatggt aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt
     2581 tcgattgatt cttcgttacg caaaaaaatg tctaattcag ctagttccaa ttgactccct
     2641 cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa
     2701 gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc
     2761 tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga
     2821 acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga
     2881 cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt
     2941 ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt
     3001 tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag
     3061 tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag
     3121 cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga
     3181 ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc
     3241 aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc
     3301 aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg
     3361 ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa
     3421 tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat
     3481 ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa
     3541 ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt
     3601 ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg
     3661 gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga
     3721 tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc
     3781 aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag
     3841 gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa
     3901 cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc
     3961 tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac
     4021 aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag
     4081 atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc
     4141 aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga
     4201 acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc
     4261 aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt
     4321 gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga
     4381 tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg
     4441 aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa
     4501 tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct
     4561 tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata
     4621 tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt
     4681 gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct
     4741 atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt
     4801 tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt
     4861 cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac
     4921 gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt
     4981 tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca
     5041 agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc
     5101 actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat
     5161 taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag
     5221 attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag
     5281 tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat
     5341 atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac
     5401 aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat
     5461 ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta
     5521 acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga
     5581 ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga
     5641 atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa
     5701 ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag
     5761 atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac
     5821 gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta
     5881 atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc
     5941 ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa
     6001 tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa
     6061 aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc
     6121 tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat
     6181 gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat
     6241 gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg
     6301 tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg
     6361 aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc
     6421 ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg
     6481 tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag
     6541 tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa
     6601 aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg
     6661 gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc
     6721 agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag
     6781 ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat
     6841 caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat
     6901 gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata
     6961 accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa
     7021 cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa
     7081 gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga
     7141 aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag
     7201 taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg
     7261 caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt
     7321 ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag
     7381 tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt
     7441 ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat
     7501 tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt
     7561 cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca
     7621 aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga
     7681 agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact
     7741 cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac
     7801 ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt
     7861 atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta
     7921 ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca
     7981 gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt
     8041 tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt
     8101 aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata
     8161 gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg
     8221 attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat
     8281 tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt
     8341 aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc
     8401 gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt
     8461 acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga
     8521 gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt
     8581 tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat
     8641 aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc
     8701 acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat
     8761 agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac
     8821 ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg
     8881 atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga
     8941 taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc
     9001 atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca
     9061 tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt
     9121 tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc
     9181 aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa
     9241 ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa
     9301 atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt
     9361 aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt
     9421 gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac
     9481 gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact
     9541 gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc
     9601 gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat
     9661 cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata
     9721 cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt
     9781 acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa
     9841 ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt
     9901 ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac
     9961 gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga
    10021 attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt
    10081 ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg
    10141 ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc
    10201 accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc
    10261 tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt
    10321 ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac
    10381 aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc
    10441 cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt
    10501 cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga
    10561 cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat
    10621 ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag
    10681 gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc
    10741 ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta
    10801 tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt
    10861 tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa
    10921 cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat
    10981 gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat
    11041 cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc
    11101 tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg
    11161 cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg
    11221 ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct
    11281 caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc
    11341 acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg
    11401 aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca
    11461 tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt
    11521 tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga
    11581 aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc
    11641 agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag
    11701 ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca
    11761 gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt
    11821 cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg
    11881 gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca
    11941 agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata
    12001 aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac
    12061 aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc
    12121 cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct
    12181 tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt
    12241 cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg
    12301 ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata
    12361 tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat
    12421 tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc
    12481 aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact
    12541 taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat
    12601 catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg
    12661 attcgaatag atatattata gaaatgagga atagctaact tctgtttctt attttgaacg
    12721 attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat
    12781 acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat
    12841 gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac
    12901 aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg
    12961 gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat
    13021 agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc
    13081 ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt
    13141 ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat
    13201 ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat
    13261 cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt
    13321 ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat
    13381 tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca
    13441 gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca
    13501 tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg
    13561 tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg
    13621 caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga
    13681 aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta
    13741 cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat
    13801 tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc
    13861 gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt
    13921 gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat
    13981 gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct
    14041 ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca
    14101 gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct
    14161 tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat
    14221 ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat
    14281 ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat
    14341 aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta
    14401 tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat
    14461 gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc
    14521 aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt
    14581 gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca
    14641 ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc
    14701 aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga
    14761 ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg
    14821 acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct
    14881 gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca
    14941 ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta
    15001 taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg
    15061 gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt
    15121 tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact
    15181 ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta
    15241 tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat
    15301 ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt
    15361 aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact
    15421 ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata
    15481 acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc
    15541 agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt
    15601 ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt
    15661 ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta
    15721 aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg
    15781 aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg
    15841 tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga
    15901 atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac
    15961 tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt
    16021 tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg
    16081 tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt
    16141 gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta
    16201 aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat
    16261 agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg
    16321 taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt
    16381 cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag
    16441 gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa
    16501 tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga
    16561 taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg
    16621 caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat
    16681 ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg
    16741 tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg
    16801 ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct
    16861 gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt
    16921 tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca
    16981 accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg
    17041 tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag
    17101 aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt
    17161 aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt
    17221 tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc
    17281 taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc
    17341 ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca
    17401 tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat
    17461 ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac
    17521 tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg
    17581 gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc
    17641 tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac
    17701 ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt
    17761 tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct
    17821 taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa
    17881 tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat
    17941 agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt
    18001 tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc
    18061 tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc
    18121 ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt
    18181 tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt
    18241 aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat
    18301 ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt
    18361 tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc
    18421 attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg
    18481 atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc
    18541 ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt
    18601 gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa
    18661 tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc
    18721 ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc
    18781 gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag
    18841 accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc
    18901 tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag
    18961 attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc
    19021 ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg
    19081 tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc
    19141 atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc
    19201 atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat
    19261 ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt
    19321 gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt
    19381 tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag
    19441 atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa
    19501 tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt
    19561 tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat
    19621 accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc
    19681 tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat attctgtagg
    19741 aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg
    19801 gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac
    19861 tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc
    19921 gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc
    19981 gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata
    20041 agcatagcca tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat
    20101 ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt
    20161 ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc
    20221 tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat
    20281 gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt
    20341 ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta
    20401 ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc
    20461 aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag
    20521 ggttgaggga gaaaaacata tgatcaggtt gttggaagga aactatagaa agatggaaaa
    20581 aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg
    20641 aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg
    20701 aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt
    20761 accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt
    20821 aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat
    20881 cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt
    20941 attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt
    21001 gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc
    21061 aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt
    21121 tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat
    21181 gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat
    21241 aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag
    21301 cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa
    21361 agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc
    21421 cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt
    21481 tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc
    21541 acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt
    21601 ttattccact ctactgaatc agaatctgat ttattattta tctagctata tcttttggat
    21661 gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag
    21721 cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt
    21781 gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct
    21841 tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat
    21901 attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt
    21961 atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac
    22021 acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt
    22081 cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta
    22141 cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat
    22201 gattgattct taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc
    22261 tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga
    22321 tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat
    22381 gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt
    22441 tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg
    22501 acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa
    22561 atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag
    22621 tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag cccttcattg
    22681 tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga
    22741 taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt
    22801 tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc
    22861 aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca
    22921 attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac
    22981 tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta
    23041 acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt
    23101 tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca
    23161 gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa
    23221 atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct
    23281 tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat
    23341 gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg
    23401 taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa
    23461 tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc
    23521 ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc
    23581 caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg
    23641 ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga
    23701 ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg
    23761 cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg
    23821 atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt
    23881 cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg
    23941 catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag
    24001 gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat
    24061 tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta
    24121 ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga
    24181 ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc
    24241 ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt
    24301 gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg
    24361 acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta
    24421 atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga
    24481 ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact
    24541 tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat
    24601 gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca
    24661 aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc
    24721 aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt
    24781 gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg
    24841 ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca
    24901 gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt
    24961 gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca
    25021 ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt
    25081 tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa
    25141 gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag
    25201 atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat
    25261 atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta
    25321 cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa
    25381 gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt
    25441 gggattcatt actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt
    25501 agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg
    25561 cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt
    25621 aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga
    25681 gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga
    25741 tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc
    25801 cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat
    25861 ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat
    25921 gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt acttgtctag
    25981 tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc
    26041 ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac
    26101 caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac
    26161 gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg attctgatca
    26221 gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc
    26281 atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt
    26341 agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc
    26401 attaaaatat acgaatccag aagataagat cattttggat tcatctgtta accataaaga
    26461 atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat
    26521 ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct
    26581 tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa
    26641 ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg
    26701 cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata
    26761 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact
    26821 tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca
    26881 tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca
    26941 tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca
    27001 tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt
    27061 ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc
    27121 ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt
    27181 gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct
    27241 agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca
    27301 ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag
    27361 cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc
    27421 attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc
    27481 tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga
    27541 tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta
    27601 tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt
    27661 tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta
    27721 taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat
    27781 gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt
    27841 cctcctagca aatagattta tgattggtat attgttactt a
//
LOCUS       DAYYJC010000028        25511 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000028 DAYYJC010000000
VERSION     DAYYJC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.028"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..2164
                     /locus_tag="ACHA9N_001173"
     CDS             104..2164
                     /locus_tag="ACHA9N_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9084646.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2247..2816
                     /locus_tag="ACHA9N_001174"
     CDS             2247..2816
                     /locus_tag="ACHA9N_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9084647.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2991..3797)
                     /locus_tag="ACHA9N_001175"
     CDS             complement(2991..3797)
                     /locus_tag="ACHA9N_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084648.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3935..5449
                     /locus_tag="ACHA9N_001176"
     CDS             3935..5449
                     /locus_tag="ACHA9N_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9084649.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5852..6835)
                     /locus_tag="ACHA9N_001177"
     CDS             complement(5852..6835)
                     /locus_tag="ACHA9N_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9084650.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6840..7487)
                     /locus_tag="ACHA9N_001178"
     CDS             complement(6840..7487)
                     /locus_tag="ACHA9N_001178"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9084651.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7572..8204)
                     /locus_tag="ACHA9N_001179"
     CDS             complement(7572..8204)
                     /locus_tag="ACHA9N_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9084652.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8220..8771)
                     /gene="coaC"
                     /locus_tag="ACHA9N_001180"
     CDS             complement(8220..8771)
                     /gene="coaC"
                     /locus_tag="ACHA9N_001180"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9084653.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8768..9505)
                     /locus_tag="ACHA9N_001181"
     CDS             complement(8768..9505)
                     /locus_tag="ACHA9N_001181"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9084654.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9502..10491)
                     /locus_tag="ACHA9N_001182"
     CDS             complement(9502..10491)
                     /locus_tag="ACHA9N_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9084655.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10749..11291
                     /locus_tag="ACHA9N_001183"
     CDS             10749..11291
                     /locus_tag="ACHA9N_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9084656.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11308..12393
                     /locus_tag="ACHA9N_001184"
     CDS             11308..12393
                     /locus_tag="ACHA9N_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084657.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12405..13211
                     /locus_tag="ACHA9N_001185"
     CDS             12405..13211
                     /locus_tag="ACHA9N_001185"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084658.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13212..14039
                     /locus_tag="ACHA9N_001186"
     CDS             13212..14039
                     /locus_tag="ACHA9N_001186"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084659.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14036..15109
                     /locus_tag="ACHA9N_001187"
     CDS             14036..15109
                     /locus_tag="ACHA9N_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084660.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15537..18233
                     /locus_tag="ACHA9N_001188"
     CDS             15537..18233
                     /locus_tag="ACHA9N_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9084661.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18298..18798
                     /locus_tag="ACHA9N_001189"
     CDS             18298..18798
                     /locus_tag="ACHA9N_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9084662.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18810..19304
                     /locus_tag="ACHA9N_001190"
     CDS             18810..19304
                     /locus_tag="ACHA9N_001190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9084663.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19325..20134
                     /locus_tag="ACHA9N_001191"
     CDS             19325..20134
                     /locus_tag="ACHA9N_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9084664.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20131..20952
                     /locus_tag="ACHA9N_001192"
     CDS             20131..20952
                     /locus_tag="ACHA9N_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9084665.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21003..23102
                     /locus_tag="ACHA9N_001193"
     CDS             21003..23102
                     /locus_tag="ACHA9N_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9084666.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23099..24217
                     /locus_tag="ACHA9N_001194"
     CDS             23099..24217
                     /locus_tag="ACHA9N_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9084667.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24256..25134
                     /locus_tag="ACHA9N_001195"
     CDS             24256..25134
                     /locus_tag="ACHA9N_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9084668.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25280..25477
                     /locus_tag="ACHA9N_001196"
     CDS             25280..25477
                     /locus_tag="ACHA9N_001196"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9084669.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8049 a         4444 c         5408 g         7610 t
ORIGIN      
        1 tctcctaata gtccatcttc ttttgatttt atttatataa tgaaatcatc ttatgaaaat
       61 tactacttca tgtttagaca agagtaggga ggactaagaa taaatgtgtt caaaaagacg
      121 ttcaaaacaa ctaaataaac acgcttgtcg tttagataag gaacggtcgt gttctgaaaa
      181 acactacgtt aagtttatga tgcgcacgat gctgatcagt ttatctgtag gtgtctatat
      241 gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc gtacgtacct ctgtttctga
      301 gaccactgat tcccctatca ctattactca tactttcact gatgcaaatg gaaaacagct
      361 acttcctgca ttgacttcct ataataaagt cagttatcct ccaacgataa accactactg
      421 gttaattcct aacaaaacat tatggacatc ggaaaccgga agcagtgtgc cgctttctat
      481 cttttccgat atgactgatc ctacaaaagg tgatcagtta aaacagatca ctgatatttt
      541 gaatcatgct ttgacagata taaaggacgg tggaagcgca caagtttctt atgtatatga
      601 aaaagattat tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga caaaagtttc
      661 gatatctgat ctggtagaaa ggctgaagaa cgcagatgga acacaaggag atatgtccaa
      721 agtaatttca aacaatggga atatcgtgga tacgactaat acaggaacat ctactatttc
      781 cttgtcctat tttgatccaa ttgctttaaa aacaatgact gcatctgctg tgatccatgt
      841 tcgtgctgat caaacagcta tcaaaggaaa agatcttcac ttaagaacag gagagagtta
      901 cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat ggaacttcag gaaataaaga
      961 agaaatcaaa atgaacggga cagtagatac tacaactccg ggagaatatc ccgttgtatt
     1021 aacttatata gactcgaaaa catcaaatgt ggcagaaaca acagctgtag tttatgtaga
     1081 agcatctgct gcttcatcga ctgtcatcac tcattttata gatagtcaaa caaataaaga
     1141 acttcaagca gaaacacata tgagtagtgt aaatcgtatt cctgatttgc gcctacctgc
     1201 tgaattttct gatcgagtgc caaatataga tttatcgact tatgaacaaa atgggaaagt
     1261 cacttctatt tctgattgga tgcaagcaaa cggattagac agaaaaagtc actggtcgga
     1321 gatccttgaa gtacttcact cagaactgat gaataatagg aagcaatcag gaggggaaat
     1381 tacctttact tatgtttata taccagacaa tcgttcatta gaaggtcatg atcttattgt
     1441 tcatccaaac gaaaaaataa acaaagaaga gttgatccaa tcagcaacaa attccttagg
     1501 taaacccgta ccgttaaagg atgtatcggt tacaatcaat gaagtaccat tggaggatgg
     1561 ttatttgacc aaacaaacag gtgattttac gattatgtat agttatcatg acccgtattc
     1621 actccaagaa ctccatgctc agtcactatt acatgtttta ccattaaaca aacagataga
     1681 tcaaaaagaa caaaaaagac gagaagaaag cttgtcagaa aaaaaacaac aaatggctca
     1741 tgcagtttct ccaacaggga aagtaaaaca aacaagaagc ctagagttag caagaatatc
     1801 tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa gctcatttgg ccccacaagt
     1861 ttctggattg gaaaatgaaa ttttagagcc taaaataaaa caacaatcag aaaaagaatt
     1921 tgttgctaaa cagtcggaag atcagaagga atcagatgac cagaaggaaa cgaagaaaaa
     1981 acgcttgtca actattgatt ccaccgatga gcatctgcag ccgcaagtac cacttcctag
     2041 tccgggaaca cccgcacctg gtggaggtat tccttcttct gctttaggag atttcatgcg
     2101 aggtatggca ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag atgactggtt
     2161 ctgatttata tctctatctg ttatagtcaa ataagtagct gagtcatatg ttatttggca
     2221 taatggagac gaggagggat agaaaaatgt tagataaaac gttaccttac gcagaaatct
     2281 ggatgacaag accacttcaa caaaatgttc caatcacgcc tttagcggaa ggttttgaat
     2341 ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga gattgagact tccgttgggg
     2401 aatttcaaac gacagatgaa gcacttgagt attttcaaca aaactttgct ccttatcctg
     2461 aagaacttga aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa gtagcaacct
     2521 gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat tggttagcag
     2581 tgaaaccaga ttaccaagga aaagggctcg caaaagcatt gactgccaga gtcttgcaag
     2641 aatttcctac cctccatacg gacggaccaa tctatcttca cacacagaca tggagccatc
     2701 aggcgattgc tctttataaa aaaatgggtt ttacttttat tgctgaaaat ctagacggca
     2761 cacctaatcc agattatgaa aaagttatgc aggttttaag taaattagat aaataacgaa
     2821 atggtgaaat aagtcctcag tattgggata aaaaacgaaa tagtcattcc tcatatctta
     2881 cttttttgag ttaatactat cggataacta atggcatgcc atacattcaa aaaagaaggg
     2941 gcagtgacaa aagtattgtc actgcccctt ctcttgtttt ttagcatgtt ttatcgtttg
     3001 atttttttag gaataaaatt cttgatgaac gtaaattttt ggtaatcgtt tcgactatag
     3061 aggtaaccaa atacactgaa aacaactgct ccaataaaat taacaaataa atccttcata
     3121 gtatccatga ttcccacgtc taagtaacca ttttggatga ttgttttatc tgttttacca
     3181 tctgcatttt gactttcaat gattgtccga gtgatatctg gaacatgata gacagtgttt
     3241 ccgtctttac ttaactccac actattgatt tgctggatga tcgtatcttt ttgcatatcg
     3301 aattgcagta atttatctgc tgaaaattcc acaaactccc acataacacc aactgtcatc
     3361 gaaaaacaga aggaaacaac tgctagtaat aaaggagtca agttcatggt ttttgtattt
     3421 ttattgagca aataaacaag ggaaaagccg acacccccag ctaaaaatcc gctgaatgca
     3481 tgtagaaaag tatcccacat tgaaaaatag ccataaaaat cacttaactc tcctaaaact
     3541 gttgaactaa aaataaaaga taacaaaatg atttctagca tatttggaat ctgtatcttt
     3601 aatatctttt ccagaaaata agggatttca aataaaatta atgtcagtaa taaaagcatt
     3661 gtatttcccc agtgttggtt ccagacctga tccactagca taataacgac gatgatacgt
     3721 aataaaatat agattccgtt tgtgattcta cctgcagttt ttgtactgtt cgtaaaaaag
     3781 gttttcaaat gattcataaa ataactcctt catgtatatc cttaattata ctaagaagcg
     3841 tgtgtttacc aaagctatat gacttaaaga aaaacagagt tgatattgtg aaaaaaatca
     3901 cttaatatag tataaggaga aaagggggat acaaatgaaa aaattagttg ctggtttatt
     3961 aacagccgtt gcacttcttg ctatgagcgc atgcggcgca gatcagaaaa atacagcgca
     4021 gacaagttct agtacgaagg aatctgccga agtatcatca ggcgcttctg aacaagtata
     4081 tacagatcca aaagaaatga aagaatctta tgatgtcgtg attattggag ctggtggtgc
     4141 agggatggca gcagctatcg aagcaaaaga tgctgggttg aatccagtaa ttttggaaaa
     4201 gatgcctgtt gccggaggaa acacatctaa atcatcaagc ggtatgaatg cttcagaaac
     4261 aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat gccttctatg aagaaacgct
     4321 aaaaggtggt ggaggaacga atgataaaga aatgcttcga tactttgtcg atcactctgc
     4381 tgatgcggtc gattggctag atcaaaacgg tatcaaactg gacaacttaa cgatcactgg
     4441 agggatgagt gaaaagagga cacatcgtcc gtctgatggc tcggctattg gtggatactt
     4501 agtggagggg ttattgaata acgtccatga gcgggaaatc cctatttttg tcaacgcaga
     4561 tgtgacagac attaagaaaa atggggaaac ggtcaatgaa gtgactgtac atgttcaagg
     4621 agaagaacca aaagaggtca ccggaaaagc aatcgtagtt accacaggcg gttttggtgc
     4681 cagcaaagaa tttattgaaa aatatcgtcc cgatttagct gattatatta cgacaaatca
     4741 agaaggatca actggagatg gtatcaccat gattgaaaaa ttaggtggac aaaccgttga
     4801 tatggataaa atacaaattc atccaactgt gcagcaggat gagggggtct tgattggtga
     4861 ggttgttcgc ggagaaggag caatcttggt cgatcaagag gggaaaagat ttgtcaatga
     4921 aatggataca agggataaag tatctgcagc aattacagca ttgcctgaaa aatcagctta
     4981 tttgattttt gaccaaggcg tgcgtgatcg tgccacagct attgaatttt atgaccaaaa
     5041 aggatatgtc acaactggaa agacaatgga agaattagca gaaaaaatcg atttaccaaa
     5101 agaaacacta gcaaaaacag ttgatggatg gaatacggat gttgcggaga aaaaagatgc
     5161 tcaattcaac cgtgccacag ccatggaaca tgacttatcg tctccgaatt attatgcgat
     5221 caaaatcgca ccaggaatcc attacacgat gggcggtgtg aaaatcaata cgaacactga
     5281 agtattagat aaagaaaacc agccaattag ggggttatat gctgctggtg aagtcacagg
     5341 tggcttacat ggaaacaacc ggatcggtgg aaattcagtt gcggagatca ttatttttgg
     5401 gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa aatgaatgaa atagaacgta
     5461 aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac cgagggtcag ctgattgaac
     5521 actgtttagt gaagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaag
     5581 taaaaacagc ttcttatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt
     5641 gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
     5701 ctctaaacac gtataaacgg cggaagcaga agaaattcct tcggaaataa gctgaaattc
     5761 acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc
     5821 tgtcccagcc tcttttttga tcagcatgat tttattcagt tatcttcaaa atgatttttc
     5881 cgatgttttt gtttgcttcc atatattcat gcgcttgttt gactgcgctg aattggaatg
     5941 tacggtcaat gattggccga atcttgccat tttctagaaa aggcccagct ttttgcataa
     6001 aagtttgtgc gagctctgct ttatactgat cacttctagg tgtcaataat gtaccaagca
     6061 gttggacacg tttcccaatc aatacaccaa ggtctatctc aggcacgatg gtcccaccta
     6121 gcataccaat caagacccaa cgtccatcaa cggctataga agctaaattc ttttcccagt
     6181 aagaagcgcc gatgaaatcc aaaataacat cgactccccg accttcagtg atttttcgta
     6241 cctcttcttg aaagtcttgt tttttatagt tgatcaattc atctgcacca agttctttac
     6301 aaaaggcgag tttttcttct gatcctgcag taacgatgat tttcgcgtga gaaagctgtc
     6361 tagctaattg gatcgctgct gttcccacac cacttgctcc tgcatggatc aagacagttt
     6421 cgccttcttg taattttcca agccagtaaa gtgtttgata agcagtcaaa aaaacttctg
     6481 agattccagc agcagaaaca tagtctagcg tatcagggag aaggattgca cgatcggcag
     6541 gcatcacagc atattcggca taaccaccta gattgactaa tccgtatact tttgtcccag
     6601 gagtaaaagt ggagatatca cttttattct caactacctc tccagctact tcgactccca
     6661 aaatagggta gggaggtttc aaagagactg attctctttt cataatatct gttcgattga
     6721 cagctgctgt atggactttg atcagtaatt cgccttgttt tggtttagga acaggcactt
     6781 caatttgttc taatacatct acagaaccag gatgcttgat tgaaatggca cgcatggatc
     6841 tattcctcct ttgcccattc ttggaatgct gtattcaact ctttgctacc ttcagaaagt
     6901 actagagtcg tacgagaaat atccgtttct tccactagag cggcaagtac agcagcaact
     6961 gtagagcgag tgatcgtttt tgatgccttc tcattaggct tagttaattg aatggaagta
     7021 acttcttcat cgttagtcaa tgtgactggt tgaagaatga caaaatctaa gttagttcgt
     7081 ttcagccatt catctgcata atgtttagta atataataat ctgtcatgga ctcaggccat
     7141 tttgctggtt catctgcata aacagcactg accatcagat agcgagagac atgcgcattt
     7201 tcagctgcga tcactgtttt gattgcgcca tccaagtcta cttgcagaag attctttcct
     7261 tgagagccgg cagcaaaaat caatacatcg atgcctttga atgcttcggt cattttttcc
     7321 acggaccaag ttaaatcaaa agagacatac gaaacattct ctttttttac taatgtttgg
     7381 gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac ttggagctaa atcttttatc
     7441 aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa ttttcatttg gatcatcctt
     7501 tcgacgtcaa atctttccat tcaatagtaa caccaaaaga gaaaaaaatc agagatcggc
     7561 tacgatgact atcagttagt aagagaaggg tgcttgaagt atcttaacaa gacattgcat
     7621 actgctgcag tcgcaagacc tgcgacgatt ccttcaggta taccacttgt aaaaataacc
     7681 gctagaatag ctgaataaac agcatttaaa tttgttccga tgactttggc ataatcttgc
     7741 tggaataaaa agtagatcat attcatcact aaaatcgtat tggtcaatga accggaaagt
     7801 ccagctaaga ataaagaaac ggaatctgct ttttgtttca ttagtttgcg cagaccttta
     7861 taaacgaaat aaggaaccac cccaatcaag atgcgaggaa taaacgcgat cagcaaggct
     7921 ttccagcttc catgttctgt tccgatgact gggataaacg gagaaaagac aaaagacaaa
     7981 ggtgtttgga taactgtgct acggatcatg ctgatcagtc caaatgtacc gccaagaaaa
     8041 gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta caggaatatg catggttgtt
     8101 gcattgattg gaccgatagg gataaaacct aaaggcgtaa cagctaataa aatcaaaata
     8161 gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt tcataagtac tccttttttc
     8221 taagcatttc tttcattaag agtcagcatg acctgttcga tgatcgtctg aatatcagct
     8281 aaggcacctt ttccaaaatc tccacaagct aaaagagatt ctcttggttc aatttcctga
     8341 tagccgacag atctcaatgt ttccagatta cgctgagtga tcgggttttg atacatattc
     8401 gtgttcatgg caggggcaat caattttggt gttcccactg gaagcgccat tgcgactgtt
     8461 gaaagaagat cgtctgcaat gccgtttgct agcttgccga taatgtttgc tgtagcaggc
     8521 gcgatgagaa acaagtcagc ttgttttgct agttcgatat gattgattac ctgcggattt
     8581 gattcttgca tgacgtctgt atggactgcg cgcttcgaca gagattgcaa ggttaatggc
     8641 gtgataaaat gtgtgctgct tttagacata atcacatcta cctgggcacc taatttgacg
     8701 aattgacttg taagatcagc gcttttgtaa gcagaaatac ttccagtaac gcctagtaag
     8761 atctttttca ttttgattcc tccagtctaa ttttctcagt aatcaatgca gcaatctcag
     8821 atttactttg agcttcttcc actgttccat ctttagataa gagatagccg tggtgtgtct
     8881 cgccatggat ttccgtcaaa tcatttgcga aaataaaatc agttttgtta tttatcagac
     8941 tattttttgc gactgccaga agagattcct ttggtacatc tactaatagt ttgaaaccaa
     9001 tgatttttgc ttttggctgc cattcacgta gataagaaat gatttttggt gttttcttca
     9061 aaacgagcac aagatgctcg gtgttagaag agatctttga aacagtggta tctgtctgtt
     9121 cattttccgc aaaccaattg tccagttctt cttgcgttga agaagtgggt aggttttttg
     9181 ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc actgactgcc atactgtgga
     9241 taatcgcatc gtagtgttct tttttcgtta gttcttctaa ttgtaaaaat aagtccagtg
     9301 cagattcgat cgtataaatc gttatattgg agtgtctttt cggtttcagt gcttgtttcg
     9361 tagtcacgta atcgattgtg gtatccggat tggcagctaa ataatcagct aatgcttgcc
     9421 ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg atcgattttt tcagtcgttc
     9481 ctccagctgt gattagaatc ttcatttatg gtcacctaca tttactagtg ttcttccttg
     9541 gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat tgatccagag taatctcttg
     9601 gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt tgccataaag aaagacgttg
     9661 atctattggt acattgacag aatcaattcc aatcaactgg atgtttcgca gaataaaagg
     9721 caagacagtc gcgtttagtt tgattccccc agcattgcca cataaagcta ctgcaccgtt
     9781 ataagagatc aatggaagaa gactgcttag ctgctcgcca cctactgtgt cgatgacata
     9841 gtcgatttgc tgttttccta aagcttttgt tgtctctggc agaaattcat caggtcgtac
     9901 gacttgcaaa gcacctagac tttttagcca tgtgacagac tcttcttttc tggaaaaggc
     9961 aatgattgac tggaatccta gtttatgtaa cagggctatc gctgtactgc ctacaccgcc
    10021 agaagcacca gtaacgacaa cttttttatc ggaggtcata ccttgacgga ttaaagcgtt
    10081 cacacacaac atagcagtaa accctgctgt accaaggatc atcgcttgac gcagcgtcat
    10141 attttttggt agaggaacta accagtctcc tggtactttt tgatattggc tataaccgcc
    10201 tggatgggtg acgccaagcc caaaaccagt agcaagtaca agatcttctt ttgaaaaacg
    10261 tggattgtta ctttggacga cagttccgga taaatcaatc cctggagtca tcggatatgt
    10321 tcgtatcacc tgaccagact cgctcattgc caatgcatct ttataattga catcagaata
    10381 ggcaattttt accaagactt cattttcagc caactcagta aatggcagat cactgattgc
    10441 tgtatggaaa acaggttcct ttattacctg aaaagatcga aaagcgttca ttcgtgagac
    10501 actcctttta aaattttctt ttatcatacc ataaagccta ctcttttatt cttgttcttc
    10561 cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca aacaactaga atttattcca
    10621 aaagataaag aaatattcag caaaaaaaat tttacaaagg gttgcctttt tcaataaatt
    10681 tcgtatacta atacgagtgg tagtaaacag gaagtttact attagaaaac ttcgattcgg
    10741 aggaaattat ggaaattggt gagaagttaa gaaatttgcg catccaaaag aatttgacac
    10801 aagaagaact aggcgaacgc acagatttgt caaaagggta tatttcccag ttggaacgag
    10861 atttaagttc accatcaatg gaaacattct tttctattct tgaagtctta ggtgtgactc
    10921 cagaagaatt ttttcatcaa gaagcagcga atctgcaagt ggtttattca aaagaagatc
    10981 atacggttta tttcgatgaa gaaaacgggt atgaattgga atggctcgtg gctgattcga
    11041 atgaaaaaga aatggagcct gtcttgctga cttttgaagg tgcaggagaa tataagacgt
    11101 ttgaaccttc attatctgaa acgtttattt ttgtattaga aggggaatta gagttaagct
    11161 taggagagac ggtctatgtg gcaaaagcag gacagtcgat ctattatcaa gcgacaaaac
    11221 aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt attgattgtt gcaacgcaat
    11281 catatttgta atataggggg taaaaacgtg gggaaaccaa tcatttcatt tgatcaagta
    11341 gtgaaacgct atgatgatga aacagtatta aaaaaagtaa gttttgagat cgaacaagga
    11401 aaattttata cactgcttgg tccttccggc tgtggtaaaa caacgatcct acgtattata
    11461 gctggattca ccgaagctac agaaggggac atctattttg aagggaaacg gttgaatgat
    11521 ttgccagcta ataagcgaca agtaaataca gtcttccaag attatgcact ttttcctcat
    11581 atgaatgttt ttgataacgt tgcttttgga ttgaagatca agaaaatgtc caaatcagat
    11641 atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc ttcctggata cgaaaaacgg
    11701 gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag cgattgcacg agcaattgta
    11761 aatgaaccga aagtattgct attagatgaa ccgttatcgg cgcttgattt gaaactacgt
    11821 acagatatgc agtatgaact gcgggatctg caacaacgat taggaatcac gtttattttt
    11881 gttactcatg accaagaaga agcgttggca atgagtgatg agatttttgt catgaataaa
    11941 gggaaaatcg tccaaagtgg tacacccgta gatatttatg atgagccaat caatcatttc
    12001 gttgctgatt ttgttgggga gagcaatatc gtggacggtg taatgatcga agataatcta
    12061 gttgagtttg ttggtaagca atttgaatgt gtcgatggcg ggatgcgtct aaatgaacca
    12121 gttgaagttg ttttacgccc agaggatttg acgatcacga caccagataa aggaaaacta
    12181 gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt atgaaatcat ctgttatgat
    12241 gaacagcaaa atgaatggat ggtccattca acgaaaaaag ctaaagaagg aagcaaagta
    12301 ggattggcct ttgagccaga agatatccat gtcatgcgtt tcaacgagtc tgaagaagaa
    12361 ttcgatgcac gtcttgacag ctacgaagaa taggggggac aagaatgaaa acaatgcgca
    12421 ggatctattc cgtcccttat attttgtggc tcggtttatt cgtgatcgcg cctgtattaa
    12481 tgatcattta tcaatctttt tttgatatga atgggcaatt cacgctgaat aattatctat
    12541 cttattttac gtcggggaca tcgctttcta tgacgatcaa ttctgtatgg tatgcatttt
    12601 tgatcacgtt tttcacgctg atcatcagtt acccgactgc ttatttttta acaaaactga
    12661 agcacaagca attatggctg atgctgatta ttttgcctac atgggtgaat ctgctgctta
    12721 aagcttacgc atttattggg attttcagtg tgcacggcag cgtgaatcat ttctttgatt
    12781 ttatcgggat cgggccgaaa cagatattat tcacagactt cagttttctg ttcgtggcaa
    12841 cgtatatcga gattccattt atgattatgc caattttcaa tgcattggaa gagatgaatc
    12901 cttctttgat cagtgctagt agagatttag gcgcgaatag ctttgagacc ttccgacgag
    12961 tgatttttcc gttattatta aatggtgtga aaagtggtgt ccaagcagta tttattcctt
    13021 ctctttcttt attcatgctg actcgtctga ttggcggaaa tcgggtgatc actttaggaa
    13081 cggctatcga acagcatttc ttggttacac aaaactgggg gatgggttct acaatcggtg
    13141 tgattttaat cattgctatg ttcatcgtga tgctcctgac aggcgagaag aaaaaaggag
    13201 gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta tttagggatc gtctttttat
    13261 tcttatatgc accgatcttc tatctgattt tttattcctt caatgaagga ggaacgatga
    13321 acagctttac aggatttact tgggaaaatt atgaagctgt ttttgaagat acacgtttga
    13381 tcattatcgt attgaatacg ttcatgctgg ctttcttatc tgcattgatc gccacgatca
    13441 tcgggacatt tggggctatg ggaatctatt acacgaaaaa acgacgtgca cggacagcgt
    13501 tgttgagcat gaataacatc ttattagtct ctcctgatgt tattatcggt gctagtttct
    13561 tgattttctt tacactacta ggattcggat taggttttca gagtgtattg ctttcccata
    13621 ttgctttcag cattccaatc gtcgtcttga tggtcttgcc aaaattgcag gaaatgaacg
    13681 attcaatggt cgatgcggct agagaccttg gtgctaataa tttccaagta gtcaaaaata
    13741 tcattttgcc ttatctttct ccaggaatca tagctggata ttttatggca tttacctatt
    13801 ctcttgatga ttttgcggtt acatttttcg tcacaggaaa tggctttacg acgctctcag
    13861 tagagatcta ttctcgtgca agacaaggaa tcagtttaga aatcaatgcg ctaagtgcgt
    13921 tagttttctt gttctccatg atattggtca ttggatacta ctttattagt aaagaaaacc
    13981 gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg ggtggcacgg ctgcgatgaa
    14041 aaaattacaa tctctgtttt taggtattct catcattatt gcggtgctgc tgcttggtgt
    14101 tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag gtacttacca tctataactg
    14161 gggagattat atcgatccta gtttgatcac gaaatttgaa aaagaatatg gatacaaagt
    14221 gaattatgaa acatttgatt ctaatgaagc gatgttcaca aaaatccagc aaggtgggac
    14281 aaactacgac atcgctatac caagtgaata tatgatccaa aaaatgatca aagaaaaact
    14341 agtacttcca ttagatcatt cgaagatcaa aggattaaat aatattgatc cacgattttt
    14401 agacttggat tttgatccag ataatcagta ttcgattcca tatttttggg gaactttagg
    14461 gatcgtatat aacgataaat tctttgatgc aaatcaaatt aagcattggg atgatttgtg
    14521 gaagccggaa ttaaaagaca gtttgatgct gattgacggt gcacgagaag ttatggggct
    14581 ttctttgaat agtttaggct attctttgaa tagtaagaat atggcacagt tgacagaagc
    14641 agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca atcgtggcag atgagatcaa
    14701 aatgtatatg atcaatgaag aagcctccgt ggcagtgaca ttttctggcg aagctgccga
    14761 tatgatgtgg gaaaatgaac atttacatta tgtgatccca ccagaaggat ccaatctttg
    14821 gtttgataat atcgttatgc caaaaaccgt gaaaaataaa gaaggtgcct atgatttcat
    14881 caactttatg ctagaaccgg aaaatgcggc acaaaatgca gagtatattg gttattctac
    14941 accaaatgaa gaagcaaaag cgttattgcc aaaagacatt tcgtcggacg aacaattcta
    15001 tccatctgat gacacgatct cccatcttga agtctacaaa gatctaggat cgaaatacct
    15061 aggcatctat aatgacctat tcttagaatt caagatgtat aggagataat ggcaaaactc
    15121 agtaagaatg gggacttatc aacgttctaa agagctaaaa atcgtccttt ttctatctga
    15181 gttttccaat cttctaccaa aagcggatga aacgccgctt tttttgtgtt attttatttt
    15241 ttctaccacg tttttctacc agagaaaagt ggacaaaacc attgtaaaat agggcccgag
    15301 acgggctagt aaatctaaga ttgaaaaagt tacagaagga taaagtaatt ggaaactatg
    15361 caaatatact taacccttct gtaatctatt aataaaacta atatatgaat ccagttcaat
    15421 atttgtagct atgaaaaaac ataaaaatca tattagataa aaactaaatt ggtagataaa
    15481 ttattttgtc agatatctga ggcaaatatt cgactatttg tgtttcaaag taaaaattgg
    15541 aatacaaata tagaatagac atattgcagc cgatagttta ttatgctttt agacacatta
    15601 aactggagga aaaaatgaaa aagagcgcta aagatcgagt ttttgatgag ctgatacgac
    15661 taacagggaa tatgtcttct gtcactgccg agaaaatttc gaagaatcta ggtatctctc
    15721 gtcagaatgc cagccattat ttgacgagat tagtggaaga taaaaaggtc gagaaattac
    15781 cgggaaagcc tgttttgtgg aagcctttag atgaatatgc ggttgtagat aatacggagc
    15841 aaataaacga agcctttcat tctgtagttg gtcatgacgg aagtctgaga gaggtcattc
    15901 aaaaatgtat agccgctgtc aaatatccgc ctaacggact cagtgtatta ataaatggag
    15961 ccacgggtgt tggaaaaagt tttttagcca caaaaatttt tgaatacgct atccatgagc
    16021 aaattattga aaaagatgca ccatttgcta tcttaaactg tgccgattat gcggacaatc
    16081 ccgaactgct ttcggcaaca ctgtttggat acaagaaggg ggcctttacg ggggctgaaa
    16141 aggataccga tggtttattg gcaacggcca acaacggcta tctttttctc gatgaggtcc
    16201 atcgcctatc gaaagaaaat caagaaaagc tctttctatt tattgatact ggaaactatc
    16261 gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt tcgttttatt ttcgccacga
    16321 cggaaaaagg tgaaaattat ttattagaca cctttgatcg gcgtattcaa attagtgtta
    16381 tgttgccaac ttttgaagat cgccccattc gagaacgatt agagctgatt caattatttt
    16441 ttcaaaatga agcggatgtt ttacaaaaag atattattgt ttcaagagaa gccttgtctt
    16501 taatgttatc tcatcccttt agtgggaata ttggtaagct gaagaatatc attaaaatca
    16561 gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt aacgattggg aaaaatgaga
    16621 tcttgattca gttaaatatg ctggaatccg aagtagaaag cttgccgatt gctagtttat
    16681 tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa accgtttgat tatgattcga
    16741 ttttggaaaa aattactcag ctgataaatg aaggtaattt caatgaaaat ttttcttcga
    16801 taaagaatga agtacagcat ttggtgcatc acattgataa ggattctttt ttatcaatga
    16861 gtagaaaatt gatgattcag atgtttagaa aggtctgggt atccgtttta ggaaaaacgt
    16921 acggattgac gacgagtgga cctgttgcag aaattattag cagatcctat gtcatttccc
    16981 caagctataa gacagactta accgggataa atcgtacatt agctgcaaat ctacctcgga
    17041 cgacttactt atgtaatcag ttcttggagc atttaccacc gctctcagaa aaggacgaga
    17101 actttctaag actgcttatt gtcgtgactt tatctgatta tgtagatgaa tccatcgagc
    17161 tgaagggcct gttgcttgct cacggagata gtacggctag cagcattcaa gcagtagtga
    17221 atcaattatg cggcaattat gtttttgaag cgttggatat gccagtagag agtaatgtgt
    17281 cagagattgt cgaatcgaca aggaatttcg tgaaacggca agtgaataca gatagtctag
    17341 tgctgatcgt agatatgggc tctttaaatc aaatttacac acagatcaag aacgatttaa
    17401 aaggggagtt acttcttctg aataatttga cgacttccat tgcacttgat attggcttga
    17461 agatgacgat gaatgcgcca tttaaacaga ttgcggagga tgcagcagac aagtattcaa
    17521 tcaatgttca atactttgaa ggcttttctc aaagtagcaa cattatcatt tcctgtatgt
    17581 cgggtttggg gatttcggac aaattgaaag atgtttttcg tcatcattta tcggatgaat
    17641 ccatcgaagt tttcacgaag gactacagag aactacgtga attaatcgac caaaatgatg
    17701 aaacttattt tgacaagaca aaattggtca tcacgacttc ggatcttcca cgttcctttt
    17761 caattcctaa tattaatgtt tatgacatct tagacggtga tggagcagcc aatttggagc
    17821 atattttgag tcctgaaatg aagccggcag aatttaaccg cttgattcag gagctagtaa
    17881 aattcttttc gttggaagga attgcggacc gtttgaactt tctgaaccca acgatcgtga
    17941 tgagtgaagt ggaaacagtc atttctaaat atgaaaatta ctatcatttc actttaaatg
    18001 gccgtgtaaa actaaatctc tatatgcata cggcattgat gatggaacgt ttattcttgg
    18061 ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa accagatgaa gatgaatttt
    18121 attatgtttc tagaagtatt tttcaatcaa tggagatgaa atataatttt aaagtcaatg
    18181 cttatgagct ttcgctgctc tatgaattat taggaccgta tattcaaaaa taaagtgtct
    18241 aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt ttttaatgtg aattaacgtg
    18301 attaaaccaa tgtttttaaa agttggcaca ggaattgctt atataaaagt gaacagaaag
    18361 aaaggaatta accatatgaa gaaaattcta atagcgactc acggtcactt agcttcaggc
    18421 tttcttagca gtattcaatt actgacgggg aaggttcaag agattacggt gattaatgct
    18481 tatgtggatg agtgtgattt tgaacaggag ttgaatgtat ttgtaaacca ggtgaacgag
    18541 caggatcaga tttttgtttt taccgattta tttggcggaa gtgtcaatca gaagattacc
    18601 aaaacctttt tggagaaaga gattgctgta actattttgt ctggctttaa tctgccaatt
    18661 ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg aggatattca ggaactggta
    18721 aataaatgcc aacaggaatt aaagatgacc attcttgatg aggtacatgt agtaaatgaa
    18781 gaggaagatt ttttttagga ggaagaaaaa tgattaaagc agtgcgaatt gatgaacgat
    18841 taattcatgg acaagtagcg atgacttgga caaagagtct aaatttaacc gggttagtag
    18901 tagcaagcga tgaggcggct accaacgata ttcaaaagat gacattgaag atggctgcac
    18961 catcgaatgt caaagtcatt attaaaacag ttgatgacgc gattcatttg ttaaaagatc
    19021 cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa tgtcaaagat gcggtaaaag
    19081 ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt ggggaatgct gggaaaatga
    19141 caccgccatc ggaagataag aaaaccttat caaaggaagt gatgttaacg acgtcagaaa
    19201 ttcaagactt aaaagaatta gttgaacttt atccagatac cgtttttcag cctacaccgg
    19261 cgatggaaaa acgttcagct atctctattt tgagtgattt ttagaaaaag gaaggagaaa
    19321 aattatgtat gatgcatttg tagtcgcttt ggccgttttt attggaattg ctggacatga
    19381 aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt gctccgttag caggagcgtt
    19441 gatgggggat ttgcagacgg ggttgattgt cggggcgtca ctagaagcca tctttatggg
    19501 tgtcgtcaat atcggaatct ctagtacggc ggagccttct ttggctgctg ggctggctac
    19561 agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt attcccattg tctttccact
    19621 agcagtatta ggattacagt taatgaatat gattttttct tttgtatgtg gaccgatggc
    19681 tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa ggaatcatca atctgcactt
    19741 ctttatgtgg tttgtccact atgcgcttta tgctcttatt ccattctttg ccgttttatt
    19801 tggagcagat gttgtcaaaa gcgtcttgga taatattcca gaggtcatca tgaacggatt
    19861 aacagtcgct ggaaatctgc tgccggcagt tgggatggcg atgctgttac ggatgctttg
    19921 gagtaaaaat attgctgtgt ggtttttctt tggggccgtt gttatggcgt atctcgagtt
    19981 accattgatt gcaattgccg cagttggaac gattattgca attgttatag cccaaagaga
    20041 tctacaacta aaaaatatat ctgttcatgc agttcaaagc gatgctgctg taaatgcaac
    20101 gcttgaccaa gaagaggagg atttctttca atgaaattag ctgatggaat ttctaaagat
    20161 gagaaaaaat tggttcggaa gatgttttgg cgctcgatga ctatgtatgc ttcggtgaat
    20221 ccggtgacga tgggcggcgg tggtttcgcc tattctatga tgcccttcat taatcatttc
    20281 tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc atacacagta tttttcgacg
    20341 accattcctt gcgcgtcctt tgtcatgggg attgcggcat cgatggagaa ggaaaattct
    20401 gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca aaacgagttt gatgggacca
    20461 ttggctggaa ttggcgattc attgttctgg ggagtttggc gtgtcgttac cgcgggacta
    20521 gcgatcaact tagccaatgc cggaaatatt ttggcaccaa ttatcttttt agttatgttc
    20581 aatataccta attacttgat gcgatactat ggcggcgttt taggctattc attaggctct
    20641 aaatatattg aaaagctgta tgcgaacggg ttaattgaaa ttctgacaaa agctgctagt
    20701 atcttgggtc tgatcatggt aggtgcaatg acgatcagta tggtcaaatt caataccgtt
    20761 ttgagctttt caatgaaggg cgaagaacta atgaatgtcc aagaaatcct cgatagtatt
    20821 tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt tcaaattact agataaaaaa
    20881 gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg gaattttatt gtcattactt
    20941 ggaatcgcat agtaggaagc tgcagctact aggctgcagt ttttttagca aggagggaaa
    21001 agatgactat taatgaagta atttattcac ccaaacaccc attttttaat gctgagttaa
    21061 acgacggaaa tgtgactact tcaaacgaat acagctacta tcggaatatt cctgatgtaa
    21121 agggattgac cttcgatgta agtaatcaaa agacattagc aaaccttgat gtttgcggga
    21181 cgattcaaac gttgaccttt tatcgagaaa atttactaac tgaggaaaaa ccaggtgttt
    21241 gggtcaataa acagctctct caaaccaatt cgttatcctt aaaggttgaa gtaaacggga
    21301 ttgtctacca tttgtcagaa tcggatcatc gtattgaagt cgatgtgatt caagactgtt
    21361 tgcctagata tattcatcat tattcgacgt ttaaggtcat tgtagtatcc tttgctccca
    21421 tactggctaa taagcgttta agtatgcttg ttcaacaggt ttacattgtg aatgaaacac
    21481 acgaaagcct tcaagtttca gtgaaagatg tgccttcgta tcaagagaag tattcggatc
    21541 agcaaaatgt gttgatttct caaaaaggaa ataaggacac gatcgcacaa caggaagtgg
    21601 caagcttcgc cattgctttt gtggacccaa atgcttttga agaagtaatg acttttcagg
    21661 aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta ttttgctcag ctatttgggc
    21721 agctgtcaat gccaaacgat agagttgtcc atctctatcg tcgtgcactt tatcaaagct
    21781 tttctagttt cggtatgaat cgacagggac aggtcgtcgg cagcaattgg ggcagctatc
    21841 ctgctactaa tcgaatttgg aataaggata tgtattacgc atcattgcca ttcaccatgt
    21901 ttgaaccgga gttatgtcaa aaaacggttc tatggtttga tcaatatggc gttaagtttc
    21961 ctggttcaaa atttacagga ggtatcaatc attctctgag taattcgtta tccagtgcgt
    22021 tactggctag tctttatttt gaacatactg gggatacaaa attctttgag cggtgtccgc
    22081 aggttctact gaatgcttca agaattattg aggacatatt agctcaacgt cggtcagggg
    22141 aaccaatgct ttttccatct gtttggttat cagacgcctt tgcacttgga aagtatcata
    22201 ccggcagcaa tctttgtctt tggaaggcct gtattggact tgcagaaatt tttgaagccc
    22261 tagaacaact gagtcttagc aagcgataca aaaacattgc ctgtaaacta aaggaatcaa
    22321 tttgtaaaca aatgactatc gatggtcctt ttggtgagca atttttggag ggaatcggtg
    22381 atgaggagaa aacaacctat tcagtgaagc actatcaaaa accgattctt gagcaagggt
    22441 taatcttttt aagtgatgtg attgtggatg gaaaaattaa tttattgatg catgatggag
    22501 aagaatcaga cactacgttg atgccttttt atcaattttt agataataaa gatcatttat
    22561 atcagaacac catggccttt tctgctagtt ctttcaatcc aacctatagc gaggaaatca
    22621 aaggcatcac ttggggatta gaatccggcg ccacttttcc tggctttatc actgttttaa
    22681 tgagcgagct gcacaaccct caagctttcg taacccgatt agaagaacta atttgtttgg
    22741 cagatttaga cggttcgtgg tggtggtggc cgtatcgctt agaggctcaa caaggagaag
    22801 tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc gggtcttttt gtcagcctaa
    22861 tgatcagtca atatttcgga ttgaaattca aaaaaggagt attgcaaatt gatcctgttg
    22921 ataatttaac gttttcttgg aagaatttga agttcggtca agccttcatt tcgattgaat
    22981 gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa gccattgaaa ataagtgata
    23041 cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca cattcaaagg agaaaaagat
    23101 gagtatacta aaaaatatta cccaatttaa aaaaatccag agtcgagcaa tcactcccga
    23161 aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca agcgagctag gaccaagccg
    23221 caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa actgtgattg cggatattac
    23281 tggaacggga ataattcgtc atatgtggat gacgattcgt gatcgaacag aatttggcag
    23341 ttttgtttta cgtgatttaa tcttaagaat ttattgggat gggtcggata gccctgctgt
    23401 tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga gagcgttgcg aggtgaactc
    23461 gttgccgata gtggttaatc ctacaggggg ctttaattcc tatttcgaaa tgcctttcca
    23521 aacatccgct aaaattacga tcgagaattt acatccgaag gatatgtcct cattttttta
    23581 tacgataaat tacgctttgg tggacgaatt ggaagaaaat agcctatatt ttcacgcaaa
    23641 ttggcatcgg gaacgcatta ccaaattaca ggaggattat aagttaattg atcgattaaa
    23701 gggtcaaggc tattacgtgg gtacttacct tgcgcttacc tgcctagaac gttattggtg
    23761 gggcgaaggc gagtttaaat tctacattga tggggatagt gagtatccga ccgtaacctc
    23821 aacaggttca gaagattatt ttggcggggc gtgggccttt catgagtacg atgagttcgg
    23881 acgtccacat gcgaaaaact attcaacagc ctttttaggg tatccttacc aatcaaatag
    23941 agatgccaca agagatcgtt ttgaaacggg aaaattgaat gccgttcatg cctttggtga
    24001 cgacgcaatg ccacgacatg gtctttatcg ttggcatctt caagacccca tctgttttac
    24061 cgaagattta tcggtcactt tacaacaaat cggcaacgat gatgttcgct tatttgagcg
    24121 gcaagacgat gttgctagtg tcgcttactg gtattcaaca gaagcttcag gaaatactaa
    24181 acgtcatttc actgatcgag aacgcttgcc gcgttaatga agtgtttttc acatattcga
    24241 agggaggaac ctatgatgat atatggggca attgaagctg gagggactaa atttgtctgt
    24301 gcagtcggta ctgaggaatt aacgattttg gaaagagtca gttttcccac aacaactcca
    24361 gttgaaacaa tgaagcaggt cattgatttt tttaaacctt ttaaggaaca attggcaggc
    24421 atcggggtcg gctgctttgg tccaattgat attcaatgca aatcaacgag tttcggtcat
    24481 atcacatcaa caccaaaact tgcttggcaa aatttcaatt ttgtaggaac gctaaagcaa
    24541 tatttcaata ttccgattga atggacaaca gatgtcaatg cggcttgcta tggtgaatat
    24601 gttgctggtt ctggcaaagg gttagcaggt gttgcatact tcactgtggg aacaggtatc
    24661 ggaggtggtg ccttgttcaa tgggactttc gtggaagggt tcagtcatcc ggaaatgggg
    24721 catatgttga tcaagcgaca tccaaaggac aatttttcag gaaactgtcc cttccaccaa
    24781 gattgtttag aggggatggc agcaggacca gcgattgaaa aacggttggg tgtaaaaggc
    24841 cagaatttat tggctgacga ttcgttttgg cagattgaag ccttttacct tgcccaatgt
    24901 gcatacaata caacactgat gttttcgcca gatagaatta tttttggcgg tggtgtaatg
    24961 aaacaagaac acatgaagaa aaaagttcag gataaatttg ttgagcttat aaatggatat
    25021 gttgaaatac caccaattga tagctacatt attacgcccg aattaggtga taatgccgga
    25081 ataatcggcg gattagcatt ggccagaaaa gcggtaagaa ataagcaacc ataattaatg
    25141 aatgtttcat tgatactcat gtcaaaagta tttaaccgag taatcaaatg atcaaatgaa
    25201 gttggataaa gcgaaaatga attcaaataa aaaggctcta agtcaaatcg tgttgttttc
    25261 attaaatcac tgaaaggaaa tgcttctgcg tttccttttt tctttttgtt gtgcgctgta
    25321 taccttgagg atattcactg gtggcgtaca cttaaatagt aatatattgt gttactttat
    25381 gtctgtggta atagttatag ttgcttacca atgtatgttc atttttcgaa atgttgactt
    25441 cataatcctt ggcttaatgt ttgaattttt ttggtgatag attttttcag ccgtataaaa
    25501 taaatcgtaa t
//
LOCUS       DAYYJC010000029        25490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000029 DAYYJC010000000
VERSION     DAYYJC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.029"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..3648
                     /locus_tag="ACHA9N_001197"
     CDS             121..3648
                     /locus_tag="ACHA9N_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9084670.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3645..7367
                     /gene="addA"
                     /locus_tag="ACHA9N_001198"
     CDS             3645..7367
                     /gene="addA"
                     /locus_tag="ACHA9N_001198"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9084671.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7436..7777
                     /locus_tag="ACHA9N_001199"
     CDS             7436..7777
                     /locus_tag="ACHA9N_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9084672.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8086..9129
                     /gene="pheS"
                     /locus_tag="ACHA9N_001200"
     CDS             8086..9129
                     /gene="pheS"
                     /locus_tag="ACHA9N_001200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9084673.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9134..11554
                     /gene="pheT"
                     /locus_tag="ACHA9N_001201"
     CDS             9134..11554
                     /gene="pheT"
                     /locus_tag="ACHA9N_001201"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9084674.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="ACHA9N_001202"
     CDS             complement(11724..12734)
                     /gene="trpS"
                     /locus_tag="ACHA9N_001202"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9084675.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12988..13725
                     /locus_tag="ACHA9N_001203"
     CDS             12988..13725
                     /locus_tag="ACHA9N_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9084676.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13739..14554
                     /locus_tag="ACHA9N_001204"
     CDS             13739..14554
                     /locus_tag="ACHA9N_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9084677.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14567..15214
                     /locus_tag="ACHA9N_001205"
     CDS             14567..15214
                     /locus_tag="ACHA9N_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9084678.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15230..15886
                     /locus_tag="ACHA9N_001206"
     CDS             15230..15886
                     /locus_tag="ACHA9N_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9084679.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16048..16869
                     /gene="racE"
                     /locus_tag="ACHA9N_001207"
     CDS             16048..16869
                     /gene="racE"
                     /locus_tag="ACHA9N_001207"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9084680.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16872..18227
                     /gene="rph"
                     /locus_tag="ACHA9N_001208"
     CDS             16872..18227
                     /gene="rph"
                     /locus_tag="ACHA9N_001208"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9084681.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18228..18746
                     /locus_tag="ACHA9N_001209"
     CDS             18228..18746
                     /locus_tag="ACHA9N_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9084682.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18853..19353
                     /gene="cbpB"
                     /locus_tag="ACHA9N_001210"
     CDS             18853..19353
                     /gene="cbpB"
                     /locus_tag="ACHA9N_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9084683.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19633..20553)
                     /locus_tag="ACHA9N_001211"
     CDS             complement(19633..20553)
                     /locus_tag="ACHA9N_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9084684.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20679..21731
                     /locus_tag="ACHA9N_001212"
     CDS             20679..21731
                     /locus_tag="ACHA9N_001212"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9084685.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21841..22596)
                     /gene="fabI"
                     /locus_tag="ACHA9N_001213"
     CDS             complement(21841..22596)
                     /gene="fabI"
                     /locus_tag="ACHA9N_001213"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9084686.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22816..23256
                     /gene="fabZ"
                     /locus_tag="ACHA9N_001214"
     CDS             22816..23256
                     /gene="fabZ"
                     /locus_tag="ACHA9N_001214"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9084687.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23371..24132)
                     /locus_tag="ACHA9N_001215"
     CDS             complement(23371..24132)
                     /locus_tag="ACHA9N_001215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9084688.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24321..25415
                     /locus_tag="ACHA9N_001216"
     CDS             24321..25415
                     /locus_tag="ACHA9N_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9084689.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8446 a         4423 c         5382 g         7239 t
ORIGIN      
        1 cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat atagaacaaa
       61 tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag gaggaaaagc
      121 atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt cttgatcgac
      181 gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct tgttcccaac
      241 tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag aagcggaaaa
      301 aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc ttggtatttg
      361 ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc tgcaatgatc
      421 ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg agaaatcaat
      481 aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac aggaaatatt
      541 caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga ccagcaactt
      601 aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac tcaacgtgca
      661 ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca agatttcaac
      721 aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta tcaattactg
      781 caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg tccttataca
      841 gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta tttccgtctt
      901 tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc accagagcag
      961 actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc tagccaaaag
     1021 aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga agctgaaaat
     1081 ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag tgaagaaaac
     1141 taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg aaatatcctt
     1201 cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca aatgatggaa
     1261 aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta ttcttatcgg
     1321 ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga agcagaaacc
     1381 gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga catcactgaa
     1441 aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa agactggcag
     1501 tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt ggaagaacag
     1561 tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa aaaaataaag
     1621 aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat ccaaagtggc
     1681 gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa cttagaagcg
     1741 gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga atatgtagta
     1801 atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac aagtggtcta
     1861 gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat caatcgtttg
     1921 gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa tgatcaagtg
     1981 tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga agtattaaat
     2041 caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat ctcttttgag
     2101 cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct tagttatgca
     2161 caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag aatcacagaa
     2221 tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga ccctaattgt
     2281 tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg attagcttta
     2341 gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt actgaattcg
     2401 tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa tgttcctgtt
     2461 tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac ttcaatttca
     2521 cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg tttgaaattg
     2581 aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta tcatgaatca
     2641 ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa tatgtccgat
     2701 gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga acttcggttt
     2761 gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg acagacgatt
     2821 caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca tcctcttcag
     2881 acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct ggaattaccg
     2941 ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga tgtagcttct
     3001 gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg ttcattcgat
     3061 gtcacagaag cttattatgg catggctatg caattattga cgtatctgga cgtagcattg
     3121 atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc atattattta
     3181 catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac attgaaaaaa
     3241 tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact agatcattca
     3301 ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa agatgaatac
     3361 caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca actattcatg
     3421 gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg agaaatcaaa
     3481 ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc attcagaagc
     3541 atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga gaaaatagat
     3601 aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat caaattcctt
     3661 taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt gatcaaggag
     3721 ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt gttcgaagag
     3781 tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg acatttactg
     3841 aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa tccgtcaata
     3901 gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta ttgcccacag
     3961 cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat tattacttaa
     4021 ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc ttgatgaagg
     4081 aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa cgatttttcc
     4141 aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat ctggtgtttt
     4201 ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa cagttaagcg
     4261 ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa caaatccgtc
     4321 ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg actcagttag
     4381 cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa cagcagatca
     4441 agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct ggtttggaac
     4501 aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa atttccaatg
     4561 aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt aaaagttatt
     4621 ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg ttagtcgaag
     4681 aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa cgggaaaaag
     4741 gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca gaaaaaacga
     4801 aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc aaagaagtaa
     4861 tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac tggctaagag
     4921 aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa tccatctatt
     4981 ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc tcaaagaaag
     5041 aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa gtcctttctt
     5101 ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc aattatgacg
     5161 aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag tttgagccgg
     5221 aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg acggatgata
     5281 tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt cgtcaattaa
     5341 ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg atagagtaca
     5401 aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta gagattttca
     5461 aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa gcgacagaaa
     5521 ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat attccgcttg
     5581 cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc agcattcgat
     5641 tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca tcaaacgaag
     5701 atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg cgagaacttg
     5761 ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg ggatatctag
     5821 agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat gcattggttg
     5881 atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc gtgcgtttca
     5941 ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca atagaagata
     6001 atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca gttgtatttt
     6061 tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat gcttttgaag
     6121 aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta tatgatacgt
     6181 taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa gaaatgcgta
     6241 aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt tcctataaaa
     6301 ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag ttagtttttg
     6361 atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt tacggattga
     6421 tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct cttcttcaac
     6481 attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt gaacaaagac
     6541 agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa gatgaaactt
     6601 tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa gcttcttcac
     6661 aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat ccagatgata
     6721 cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc cgtcaatttc
     6781 gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg caagtgtcag
     6841 cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta gagatgccaa
     6901 ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta gtagacgaaa
     6961 aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca gaacttggtc
     7021 aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc atgctgcttc
     7081 ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta atccacggaa
     7141 tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt aaaacagatt
     7201 ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa tatcaaggac
     7261 agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta gaaaatgttt
     7321 tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta taattgagca
     7381 aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga gtatgatgga
     7441 gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct ctattttagg
     7501 aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc aacgctttgt
     7561 tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac gcttgaagga
     7621 attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga gccgttcaga
     7681 atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa tacaagtttg
     7741 gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa atcttgtaaa
     7801 ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg gctaaaccag
     7861 ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg aagttcactt
     7921 tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa gagtgcggtg
     7981 taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt aacaggaatg
     8041 aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc tctacaagca
     8101 caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca agatttaaat
     8161 gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac agaagtatta
     8221 cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt cgcaaatgag
     8281 atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga agaagaagcg
     8341 ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag aaaagtaaaa
     8401 catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat ttttgtgggt
     8461 atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa tttcgaacgg
     8521 atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta tatttcagac
     8581 gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga aaaacatgat
     8641 ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag agatacagat
     8701 gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga taaaaacatc
     8761 acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt tggggaagat
     8821 cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt agaagtagat
     8881 gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac tggatggatc
     8941 gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg aatcgatcca
     9001 gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat gttgcggtat
     9061 ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag tcagttcaag
     9121 ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag atttatcgaa
     9181 tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag tagagggagt
     9241 aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg aatgcgtacc
     9301 acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag acgaactgtc
     9361 tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg ttgcacttcc
     9421 taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg gccaagtatc
     9481 aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta taccgaaaga
     9541 atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc cagtttttcc
     9601 ttatttggat atggatgatt cgattattga attatctatc acaccgaatc gtgcggatgc
     9661 attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga ctcctacatt
     9721 cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc aagtggaagt
     9781 tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag taagaatcca
     9841 gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc caatcaacaa
     9901 tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac atgcatttga
     9961 ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa atgaaacatt
    10021 agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca ttactaacgg
    10081 caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga tcacgaatga
    10141 aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc gtcgtacagc
    10201 aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa tcaaccatgc
    10261 gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg cgggtgggga
    10321 agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat cagtgaagat
    10381 ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag aagtaaatga
    10441 gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt ccgtagtagt
    10501 accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag ttgctcgtat
    10561 ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg ctggaagctt
    10621 gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa gcggattgag
    10681 cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg ttataaatga
    10741 aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat tacgaatgaa
    10801 tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa atacagagat
    10861 tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta cacatctgcc
    10921 gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa aagattggca
    10981 gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa acttatttga
    11041 acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga aggaaatgca
    11101 tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg tcggtcaagt
    11161 gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg agtttgattt
    11221 atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga caaaattccc
    11281 agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc aggaattaac
    11341 agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat ttgatgtcta
    11401 tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga cattcgtcaa
    11461 cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag tcgaaaaagc
    11521 gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta agtaacgatc
    11581 aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc gtcacatctc
    11641 tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag gcctttacag
    11701 cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc tgattttact
    11761 tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg taagatttcc
    11821 ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg tgcaaattca
    11881 gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc gccgcggcgg
    11941 taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc caagtaggtg
    12001 aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt tggatcagta
    12061 tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata aataccgttt
    12121 cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc cattcctttt
    12181 ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt gaagctttgt
    12241 acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg tacaagattc
    12301 gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa tcctgtaggc
    12361 acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt gcgccgaacg
    12421 cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc cggaatttgt
    12481 gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata gtctaaagca
    12541 acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt cagtgcttgc
    12601 atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc cactcgggtt
    12661 tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc tcccgttaaa
    12721 ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac ttaatatctt
    12781 accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa gaggataaaa
    12841 taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc taagaaactt
    12901 tacaaattac taaactatca gatataatgt attttattat acatctttaa aagaaatctt
    12961 actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca cgtggataaa
    13021 tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa aggagaagtt
    13081 gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg tatcaatgga
    13141 ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca tgataaaaag
    13201 acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt caatttgtac
    13261 ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt gaagcaagac
    13321 caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat gttagataag
    13381 aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc aatcgccaga
    13441 ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc tttggatccg
    13501 gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg tatgtcaatg
    13561 attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt tatcttcatg
    13621 gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga aaatccaaat
    13681 gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga ggaaaaagat
    13741 gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc ttgcactagc
    13801 cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca aatctaccaa
    13861 tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg atattgagag
    13921 cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa agatccttgg
    13981 tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc cattattgaa
    14041 aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac gaaaaaaaca
    14101 ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa agaaaggaag
    14161 ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag tcaaaggttc
    14221 tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag aattagaaaa
    14281 ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga ctacggataa
    14341 tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg gtggaacgtt
    14401 tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct tgaaagctgt
    14461 taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct atcaaaaatg
    14521 gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga gcgaattgat
    14581 acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt cgagtgtctt
    14641 agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat tatcaaagaa
    14701 taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca ttccattatt
    14761 aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc tagatggatt
    14821 ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg aaacagtacg
    14881 ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt ctggttttac
    14941 ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg ttatcccgcc
    15001 attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa tcgtagctgg
    15061 tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata ccttcgatac
    15121 gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt acttaatggt
    15181 ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca tggattttag
    15241 cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg ttactgttga
    15301 agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag gtgtgattcg
    15361 ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata ttatacggaa
    15421 tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa tcggcattca
    15481 gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag ctatgttgtc
    15541 ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag caggattatc
    15601 aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag catttaaatc
    15661 aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt cattagcagt
    15721 aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac agaacaccaa
    15781 ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat gttacgcgct
    15841 ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga tgattctttt
    15901 agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga aaaaaagaga
    15961 tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag tacacaatag
    16021 ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg ccctatcgga
    16081 tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa acaattaccg
    16141 aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc tagacccgcg
    16201 gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca aggaatcaag
    16261 atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat caaagctgct
    16321 ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt aaaaaaaaca
    16381 caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca agcttatgaa
    16441 aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg tcccaaattt
    16501 gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat tgtggcagaa
    16561 acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg cacccattat
    16621 ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact gatcgattct
    16681 ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt gagcaattca
    16741 ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa acttttcgag
    16801 gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat cgaattggga
    16861 ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa ctggagcttc
    16921 aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt gatacaacag
    16981 ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa acaggaacag
    17041 gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga aatcgtagag
    17101 aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt attggtcgtt
    17161 ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc gtagactgcg
    17221 atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca tttgtggcat
    17281 tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat ccaataaaag
    17341 aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc acagatttgg
    17401 attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca gaatctggaa
    17461 aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa cagttgaatg
    17521 aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa aaacatgctt
    17581 tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca atcatcattg
    17641 cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa gcgggatatc
    17701 atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact ggcagcacat
    17761 ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat cagccagtct
    17821 tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga atctattctg
    17881 cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta ttatatgaat
    17941 tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta gtattcgcgg
    18001 cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta gcccgtatac
    18061 catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt aagaaacaaa
    18121 cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct caagcaatga
    18181 aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg cggtatttag
    18241 tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat cgttatcgtt
    18301 ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct gacccattat
    18361 gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc gaacagaaaa
    18421 aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta tcgaatgtcc
    18481 gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat atggtgtttt
    18541 ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt ttgaatcctg
    18601 gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt atcgaaagtt
    18661 tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt gaagagttgg
    18721 ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata aaaataagct
    18781 ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg aaatagaaat
    18841 ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa tcaggaaacg
    18901 tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt gtcccatggt
    18961 ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa agatgatcac
    19021 tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt gcaaacaatc
    19081 agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga tgttccgaca
    19141 attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc gcctttttta
    19201 ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga gctgcttaaa
    19261 gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc gaaaatcgaa
    19321 gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa gataatcaaa
    19381 aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga ggctgggaca
    19441 aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc aaatttttgt
    19501 gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac gtgtttagag
    19561 cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga ttcgttgcat
    19621 cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga aaccagaagc
    19681 tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt gttttccgtt
    19741 caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca ctaatccaaa
    19801 gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac gatttactcg
    19861 atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa tatctaaaag
    19921 tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat tcgggatgaa
    19981 atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag atacgacggt
    20041 tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct gttgttccat
    20101 gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa gaccgattgc
    20161 cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga tagcagataa
    20221 aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta aaagactatg
    20281 taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat taaaagactt
    20341 atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca aaaaaatcag
    20401 atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc cgtcccaaaa
    20461 ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc cagaagagtc
    20521 tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa aatttgattc
    20581 tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac tataatgaag
    20641 gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt ggagaaaata
    20701 acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct tatatcaaaa
    20761 ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga gaattgacag
    20821 aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt cagcaatgtt
    20881 ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt cgacctgaac
    20941 aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg gtccctgaaa
    21001 agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt gtgccgccct
    21061 atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt gaaaacattg
    21121 gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta ggagcgtatt
    21181 tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt gctgctccta
    21241 aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt ccaggaacag
    21301 aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca catacgaaaa
    21361 tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa ttcatcactc
    21421 cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag ttagctgaag
    21481 aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta gatcaatttg
    21541 cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg atttatcaca
    21601 ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca aaacggttgt
    21661 atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc tggattgaag
    21721 tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa atcaggaaga
    21781 gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg caacaaaagt
    21841 ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc cgctggaaag
    21901 tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac cttgctgatc
    21961 aggcgtacga gattcagata actggatcag tttttgatag tctttaacac ctgtgacagc
    22021 taatgttttg attgccccag ctgagattcc attgacccga attcctttag gcgagaactc
    22081 agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca tcatgttata
    22141 attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc tgtttggtgc
    22201 aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga tatcttgagc
    22261 taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt ctttgttggc
    22321 gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga tggcattcat
    22381 tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg cttcttcgcc
    22441 gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga taatttctgc
    22501 cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt tagcgacgcc
    22561 cataatgacg attttttttc cttctagaaa tgacataatg actctccttt gtttctgata
    22621 aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct tgatttctta
    22681 attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa aaaagattgc
    22741 taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca attgcctgaa
    22801 agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga attgattcct
    22861 aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg aaaaaagatc
    22921 gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt tcctgacaat
    22981 ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc gatacttatc
    23041 ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa taaggccaaa
    23101 ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat caccaaaatg
    23161 aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa agtctgtgag
    23221 tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat atagcaaaac
    23281 aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta gcatacgttg
    23341 tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt gcagtatatt
    23401 gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt cgatatttgc
    23461 aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat aatggccatc
    23521 tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat agtcgtcttt
    23581 taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc tgccgttact
    23641 gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa ggtggttgtt
    23701 gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat cgcctttagg
    23761 atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc cgttgtagta
    23821 accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt cgggaaataa
    23881 tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat tttcggcgac
    23941 atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg cagtcgtgct
    24001 ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag tcgtgttgtt
    24061 tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta aaataacttt
    24121 atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa tgataacaaa
    24181 atagagggaa aatacctact taaattattg gaatttatca tttattttaa aagaaaaaga
    24241 aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa aaacgaaaag
    24301 atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca gaacggtcaa
    24361 catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat aaacgggttt
    24421 ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa tttaaagaag
    24481 tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat ccagcgttat
    24541 ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg ggtgcctacg
    24601 gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg cgcaatcctt
    24661 ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac aacatcatga
    24721 gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct catcgatact
    24781 ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa tataatttga
    24841 atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct acgcaagatg
    24901 gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg aagcatttaa
    24961 tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt gaagaagaat
    25021 tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta gatacggaag
    25081 aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt tatcgagggg
    25141 atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa gataaagatt
    25201 tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac aatgaagggt
    25261 acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac gacggacgtg
    25321 tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt ttgaaaccat
    25381 ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc agaaataatc
    25441 cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
//
LOCUS       DAYYJC010000030        25257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000030 DAYYJC010000000
VERSION     DAYYJC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.030"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..809
                     /locus_tag="ACHA9N_001217"
     CDS             162..809
                     /locus_tag="ACHA9N_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9084690.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    938..1154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1202..3847
                     /locus_tag="ACHA9N_001218"
     CDS             1202..3847
                     /locus_tag="ACHA9N_001218"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9084691.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4157..5470
                     /locus_tag="ACHA9N_001219"
     CDS             4157..5470
                     /locus_tag="ACHA9N_001219"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9084692.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5460..6113
                     /locus_tag="ACHA9N_001220"
     CDS             5460..6113
                     /locus_tag="ACHA9N_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9084693.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6168..6719
                     /gene="radC"
                     /locus_tag="ACHA9N_001221"
                     /pseudo
     CDS             6168..6719
                     /gene="radC"
                     /locus_tag="ACHA9N_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6850..7935
                     /locus_tag="ACHA9N_001222"
     CDS             6850..7935
                     /locus_tag="ACHA9N_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084694.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7932..9824
                     /locus_tag="ACHA9N_001223"
     CDS             7932..9824
                     /locus_tag="ACHA9N_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM9084695.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9831..10208
                     /locus_tag="ACHA9N_001224"
     CDS             9831..10208
                     /locus_tag="ACHA9N_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM9084696.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10362..11144
                     /locus_tag="ACHA9N_001225"
     CDS             10362..11144
                     /locus_tag="ACHA9N_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM9084697.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11270..12001)
                     /gene="erm(A)"
                     /locus_tag="ACHA9N_001226"
     CDS             complement(11270..12001)
                     /gene="erm(A)"
                     /locus_tag="ACHA9N_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM9084698.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12059..12118)
                     /locus_tag="ACHA9N_001227"
     CDS             complement(12059..12118)
                     /locus_tag="ACHA9N_001227"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM9084699.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12510..13172
                     /locus_tag="ACHA9N_001228"
     CDS             12510..13172
                     /locus_tag="ACHA9N_001228"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9084700.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13407..13550
                     /locus_tag="ACHA9N_001229"
                     /pseudo
     CDS             13407..13550
                     /locus_tag="ACHA9N_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            13700..13900
                     /locus_tag="ACHA9N_001230"
     CDS             13700..13900
                     /locus_tag="ACHA9N_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9084701.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14154..15833)
                     /locus_tag="ACHA9N_001231"
     CDS             complement(14154..15833)
                     /locus_tag="ACHA9N_001231"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9084702.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15835..16047)
                     /locus_tag="ACHA9N_001232"
     CDS             complement(15835..16047)
                     /locus_tag="ACHA9N_001232"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9084703.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16440..18170
                     /gene="efrA"
                     /locus_tag="ACHA9N_001233"
     CDS             16440..18170
                     /gene="efrA"
                     /locus_tag="ACHA9N_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9084704.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18167..19927
                     /gene="efrB"
                     /locus_tag="ACHA9N_001234"
     CDS             18167..19927
                     /gene="efrB"
                     /locus_tag="ACHA9N_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9084705.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20022..20219
                     /locus_tag="ACHA9N_001235"
     CDS             20022..20219
                     /locus_tag="ACHA9N_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084706.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20372..20857
                     /locus_tag="ACHA9N_001236"
     CDS             20372..20857
                     /locus_tag="ACHA9N_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9084707.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20961..21554)
                     /gene="clpP"
                     /locus_tag="ACHA9N_001237"
     CDS             complement(20961..21554)
                     /gene="clpP"
                     /locus_tag="ACHA9N_001237"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9084708.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21734..22261
                     /locus_tag="ACHA9N_001238"
     CDS             21734..22261
                     /locus_tag="ACHA9N_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084709.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22352..23089
                     /locus_tag="ACHA9N_001239"
     CDS             22352..23089
                     /locus_tag="ACHA9N_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9084710.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23093..24010
                     /gene="miaA"
                     /locus_tag="ACHA9N_001240"
     CDS             23093..24010
                     /gene="miaA"
                     /locus_tag="ACHA9N_001240"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9084711.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            24012..25241
                     /gene="hflX"
                     /locus_tag="ACHA9N_001241"
     CDS             24012..25241
                     /gene="hflX"
                     /locus_tag="ACHA9N_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9084712.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8403 a         4116 c         5308 g         7430 t
ORIGIN      
        1 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
       61 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
      121 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
      181 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
      241 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
      301 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
      361 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
      421 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
      481 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
      541 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
      601 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
      661 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
      721 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
      781 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
      841 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
      901 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
      961 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     1021 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     1081 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     1141 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     1201 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     1261 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     1321 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     1381 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     1441 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     1501 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     1561 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     1621 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     1681 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     1741 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     1801 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     1861 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     1921 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     1981 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     2041 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     2101 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     2161 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     2221 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     2281 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     2341 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     2401 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     2461 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     2521 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     2581 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     2641 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     2701 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     2761 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     2821 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     2881 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     2941 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     3001 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     3061 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     3121 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     3181 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     3241 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     3301 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     3361 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     3421 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     3481 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     3541 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     3601 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     3661 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     3721 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     3781 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     3841 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     3901 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     3961 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     4021 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     4081 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     4141 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     4201 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     4261 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     4321 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     4381 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     4441 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     4501 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     4561 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     4621 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     4681 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     4741 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     4801 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     4861 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     4921 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     4981 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     5041 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     5101 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     5161 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     5221 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     5281 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     5341 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     5401 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     5461 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     5521 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     5581 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     5641 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     5701 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     5761 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     5821 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     5881 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     5941 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     6001 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     6061 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     6121 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     6181 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     6241 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     6301 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     6361 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     6421 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     6481 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     6541 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     6601 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     6661 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     6721 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     6781 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     6841 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     6901 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     6961 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     7021 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
     7081 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
     7141 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
     7201 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
     7261 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
     7321 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
     7381 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
     7441 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
     7501 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
     7561 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
     7621 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
     7681 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
     7741 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
     7801 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
     7861 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
     7921 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
     7981 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
     8041 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
     8101 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
     8161 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
     8221 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
     8281 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
     8341 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
     8401 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
     8461 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
     8521 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
     8581 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
     8641 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
     8701 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
     8761 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
     8821 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
     8881 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
     8941 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
     9001 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
     9061 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
     9121 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
     9181 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
     9241 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
     9301 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
     9361 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
     9421 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
     9481 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
     9541 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
     9601 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
     9661 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
     9721 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
     9781 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
     9841 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
     9901 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
     9961 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    10021 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    10081 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    10141 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    10201 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    10261 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    10321 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    10381 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    10441 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    10501 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    10561 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    10621 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    10681 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    10741 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    10801 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    10861 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    10921 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    10981 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    11041 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    11101 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    11161 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    11221 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    11281 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    11341 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    11401 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    11461 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    11521 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    11581 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    11641 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    11701 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    11761 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    11821 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    11881 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    11941 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    12001 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    12061 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    12121 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    12181 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    12241 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    12301 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    12361 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    12421 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    12481 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    12541 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    12601 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    12661 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    12721 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    12781 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    12841 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    12901 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    12961 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    13021 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    13081 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    13141 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    13201 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    13261 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    13321 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    13381 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    13441 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    13501 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    13561 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    13621 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    13681 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    13741 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    13801 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    13861 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    13921 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    13981 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    14041 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    14101 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    14161 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    14221 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    14281 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    14341 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    14401 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    14461 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    14521 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    14581 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    14641 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    14701 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    14761 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    14821 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    14881 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    14941 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    15001 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    15061 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    15121 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    15181 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    15241 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    15301 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    15361 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    15421 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    15481 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    15541 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    15601 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    15661 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    15721 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    15781 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    15841 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    15901 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    15961 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    16021 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    16081 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    16141 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    16201 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    16261 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    16321 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    16381 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    16441 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    16501 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    16561 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    16621 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    16681 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    16741 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    16801 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    16861 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    16921 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    16981 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    17041 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    17101 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    17161 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    17221 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    17281 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    17341 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    17401 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    17461 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    17521 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    17581 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    17641 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    17701 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    17761 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    17821 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    17881 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    17941 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    18001 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    18061 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    18121 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    18181 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    18241 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    18301 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    18361 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    18421 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    18481 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    18541 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    18601 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    18661 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    18721 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    18781 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    18841 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    18901 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    18961 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    19021 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    19081 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    19141 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    19201 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    19261 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    19321 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    19381 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    19441 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    19501 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    19561 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    19621 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    19681 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    19741 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    19801 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    19861 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    19921 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    19981 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    20041 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    20101 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    20161 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    20221 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    20281 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    20341 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    20401 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    20461 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    20521 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    20581 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    20641 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    20701 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    20761 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    20821 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    20881 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    20941 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    21001 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    21061 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    21121 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    21181 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    21241 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    21301 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    21361 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    21421 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    21481 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    21541 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    21601 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    21661 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    21721 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    21781 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    21841 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    21901 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    21961 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    22021 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    22081 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    22141 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    22201 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    22261 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    22321 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    22381 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    22441 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    22501 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    22561 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    22621 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    22681 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    22741 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    22801 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    22861 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    22921 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    22981 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    23041 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    23101 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    23161 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    23221 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    23281 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    23341 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    23401 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    23461 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    23521 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    23581 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    23641 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    23701 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    23761 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    23821 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    23881 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    23941 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    24001 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    24061 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    24121 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    24181 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    24241 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    24301 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    24361 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    24421 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    24481 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    24541 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    24601 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    24661 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    24721 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    24781 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    24841 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    24901 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    24961 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    25021 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    25081 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    25141 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    25201 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAYYJC010000031        24575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000031 DAYYJC010000000
VERSION     DAYYJC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.031"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACHA9N_001242"
     CDS             complement(152..550)
                     /locus_tag="ACHA9N_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9084713.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACHA9N_001243"
     CDS             complement(547..1320)
                     /locus_tag="ACHA9N_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9084714.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACHA9N_001244"
     CDS             complement(1317..2000)
                     /locus_tag="ACHA9N_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9084715.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACHA9N_001245"
     CDS             complement(2167..3054)
                     /locus_tag="ACHA9N_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084716.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACHA9N_001246"
     CDS             complement(3051..3920)
                     /locus_tag="ACHA9N_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084717.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACHA9N_001247"
     CDS             complement(4057..5343)
                     /locus_tag="ACHA9N_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084718.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACHA9N_001248"
     CDS             5616..6194
                     /locus_tag="ACHA9N_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9084719.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACHA9N_001249"
     CDS             6194..7123
                     /locus_tag="ACHA9N_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084720.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACHA9N_001250"
     CDS             7120..8856
                     /locus_tag="ACHA9N_001250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9084721.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA9N_001251"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACHA9N_001251"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9084722.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACHA9N_001252"
     CDS             complement(9752..10261)
                     /locus_tag="ACHA9N_001252"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9084723.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA9N_001253"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACHA9N_001253"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9084724.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACHA9N_001254"
     CDS             complement(11562..12653)
                     /locus_tag="ACHA9N_001254"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9084725.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA9N_001255"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACHA9N_001255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9084726.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA9N_001256"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACHA9N_001256"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9084727.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA9N_001257"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACHA9N_001257"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9084728.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ACHA9N_001258"
     CDS             complement(16616..17590)
                     /locus_tag="ACHA9N_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9084729.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ACHA9N_001259"
     CDS             complement(17571..19454)
                     /locus_tag="ACHA9N_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9084730.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ACHA9N_001260"
     CDS             complement(19472..21697)
                     /locus_tag="ACHA9N_001260"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9084731.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ACHA9N_001261"
     CDS             21812..22846
                     /locus_tag="ACHA9N_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084732.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ACHA9N_001262"
     CDS             complement(22913..23926)
                     /locus_tag="ACHA9N_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9084733.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ACHA9N_001263"
     CDS             complement(24004..24192)
                     /locus_tag="ACHA9N_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9084734.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DAYYJC010000032        24092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000032 DAYYJC010000000
VERSION     DAYYJC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.032"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..1068
                     /locus_tag="ACHA9N_001264"
     CDS             157..1068
                     /locus_tag="ACHA9N_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9084735.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1324..2241)
                     /gene="rnhC"
                     /locus_tag="ACHA9N_001265"
     CDS             complement(1324..2241)
                     /gene="rnhC"
                     /locus_tag="ACHA9N_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9084736.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2443..2880
                     /gene="zapA"
                     /locus_tag="ACHA9N_001266"
     CDS             2443..2880
                     /gene="zapA"
                     /locus_tag="ACHA9N_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9084737.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            2938..3485
                     /locus_tag="ACHA9N_001267"
                     /pseudo
     CDS             2938..3485
                     /locus_tag="ACHA9N_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3600..5960
                     /locus_tag="ACHA9N_001268"
     CDS             3600..5960
                     /locus_tag="ACHA9N_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9084738.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6047..6361
                     /gene="trxA"
                     /locus_tag="ACHA9N_001269"
     CDS             6047..6361
                     /gene="trxA"
                     /locus_tag="ACHA9N_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9084739.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6543..8321
                     /gene="uvrC"
                     /locus_tag="ACHA9N_001270"
     CDS             6543..8321
                     /gene="uvrC"
                     /locus_tag="ACHA9N_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9084740.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8466..9365
                     /locus_tag="ACHA9N_001271"
     CDS             8466..9365
                     /locus_tag="ACHA9N_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084741.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9358..10620
                     /locus_tag="ACHA9N_001272"
     CDS             9358..10620
                     /locus_tag="ACHA9N_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084742.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10783..12681
                     /locus_tag="ACHA9N_001273"
     CDS             10783..12681
                     /locus_tag="ACHA9N_001273"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9084743.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(12980..13699)
                     /locus_tag="ACHA9N_001274"
     CDS             complement(12980..13699)
                     /locus_tag="ACHA9N_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9084744.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13889..13936
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14036..14368
                     /locus_tag="ACHA9N_001275"
     CDS             14036..14368
                     /locus_tag="ACHA9N_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084745.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14355..16358
                     /locus_tag="ACHA9N_001276"
     CDS             14355..16358
                     /locus_tag="ACHA9N_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9084746.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16377..16847
                     /locus_tag="ACHA9N_001277"
     CDS             16377..16847
                     /locus_tag="ACHA9N_001277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9084747.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16868..17452
                     /locus_tag="ACHA9N_001278"
     CDS             16868..17452
                     /locus_tag="ACHA9N_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM9084748.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17467..18453
                     /locus_tag="ACHA9N_001279"
     CDS             17467..18453
                     /locus_tag="ACHA9N_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9084749.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18443..18754
                     /locus_tag="ACHA9N_001280"
     CDS             18443..18754
                     /locus_tag="ACHA9N_001280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9084750.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18776..20557
                     /locus_tag="ACHA9N_001281"
     CDS             18776..20557
                     /locus_tag="ACHA9N_001281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9084751.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20550..21926
                     /locus_tag="ACHA9N_001282"
     CDS             20550..21926
                     /locus_tag="ACHA9N_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9084752.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21933..22568
                     /locus_tag="ACHA9N_001283"
     CDS             21933..22568
                     /locus_tag="ACHA9N_001283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9084753.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22598..23953
                     /locus_tag="ACHA9N_001284"
     CDS             22598..23953
                     /locus_tag="ACHA9N_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9084754.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         7930 a         4153 c         5274 g         6735 t
ORIGIN      
        1 tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat
       61 tttctacggt tatatctttt acagcccctt ttttgcgtac ttaagctgat tttaagtttt
      121 gacccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt
      181 gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag
      241 atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt
      301 gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca
      361 gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt
      421 tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc
      481 ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat
      541 gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat
      601 gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta
      661 gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga
      721 gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac
      781 tctacggaat atgcgaatga agcaactgga atcacgacat attattacta tggacaagag
      841 gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga
      901 atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag
      961 atgggttata tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag
     1021 cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata aataagccca
     1081 gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa
     1141 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag
     1201 gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
     1261 ttggcatgag aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt
     1321 ccattatttt tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata
     1381 tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt
     1441 cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc
     1501 caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc
     1561 tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt
     1621 aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat
     1681 tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt
     1741 gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat
     1801 ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc
     1861 tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac
     1921 tgttagcgga ccaaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg
     1981 actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc
     2041 ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga
     2101 tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg
     2161 tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc
     2221 tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa
     2281 ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat
     2341 ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt
     2401 acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca
     2461 cgctacaaag cagtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat
     2521 catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca
     2581 ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa
     2641 ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa
     2701 atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa
     2761 gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa
     2821 gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa
     2881 gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg
     2941 ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg
     3001 tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat
     3061 ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca
     3121 acatcaaagt tagcattttt tgatcaggta tttgcttttc acctagatga agctttttat
     3181 gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg
     3241 gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga
     3301 ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc
     3361 tttgttccat tagtgtggtc caaaatcaat tcggcgagca tagtttcgct cgttttatcg
     3421 ttgagagaac gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga
     3481 attaaggaaa atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa
     3541 ggctgcgtag cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa
     3601 tgaataaccg aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat
     3661 ttgtagtgac tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac
     3721 aaacgatcac aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag
     3781 gcgggatccc tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg
     3841 gagcagactt gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg
     3901 aattgaatcg tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata
     3961 tgtgggcaga tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg
     4021 atgaagacgg acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca
     4081 ttcggcgaag tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg
     4141 caaagtattt aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca
     4201 aacaagaata ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga
     4261 ctttatttat tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa
     4321 tcgctgaacg aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc
     4381 atcgtcaaga aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa
     4441 aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg
     4501 tcgtcttgaa acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca
     4561 tcacaatcgg aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa
     4621 cgatcacgtt gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc
     4681 ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg
     4741 aacagtcgat tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata
     4801 ttttatcaaa agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag
     4861 atccgcaaga aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg
     4921 cttatgtgat ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa
     4981 atacgattaa tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc
     5041 tgattggcgt tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata
     5101 cagaagttat tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa
     5161 tgatcacaga tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc
     5221 attttgtttc tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct
     5281 ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag
     5341 aggcggaaga aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg
     5401 ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac
     5461 atcaagaaga aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt
     5521 tgaaaccggg agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta
     5581 aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg
     5641 agctgacgcc tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat
     5701 ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag
     5761 cgctaagtga ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga
     5821 ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa
     5881 accatcggag cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga
     5941 caattgttaa atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat
     6001 agaaatgcta tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat
     6061 tacagataag acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc
     6121 aacttggtgt ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata
     6181 tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca
     6241 acaattcggt atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga
     6301 aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata
     6361 aaaacttaca gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt
     6421 ttgcggttga gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc
     6481 tggattgttt ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc
     6541 agatgaacga acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact
     6601 tgatgaaaga taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc
     6661 gtgtccgctc atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa
     6721 tcgaagactt tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca
     6781 atctgataaa gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc
     6841 ctttccttaa gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa
     6901 aagacaaagc actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga
     6961 agatcctcga tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt
     7021 gtctttacta tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag
     7081 tctatacgaa aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg
     7141 agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg
     7201 cagaataccg agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa
     7261 atactgattt actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg
     7321 tccaagtgtt tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt
     7381 tctataacga agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg
     7441 agcatttcat tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag
     7501 ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat
     7561 tggcgaataa gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac
     7621 aggaacggac gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa
     7681 tacgtatcga gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg
     7741 tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag
     7801 tcaacggacc ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag
     7861 tgattcgcga agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag
     7921 tagatgtggc aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca
     7981 tggcaaaaaa cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag
     8041 taccgcttga gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc
     8101 atcgattcgc aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat
     8161 tagatgaaat tgaaaggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat
     8221 cgttgaaaaa tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa
     8281 atgtcgcaca aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa
     8341 gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta
     8401 aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg
     8461 aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc
     8521 atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg
     8581 ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt
     8641 tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg
     8701 aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta
     8761 gagggaaatc aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg
     8821 taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc
     8881 agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg
     8941 ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg
     9001 gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt
     9061 tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat
     9121 catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa
     9181 tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag
     9241 atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt
     9301 ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg
     9361 cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc
     9421 ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt
     9481 gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt
     9541 cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa
     9601 aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag
     9661 aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta
     9721 ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt
     9781 tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat
     9841 tttgattcaa aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta
     9901 agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca
     9961 caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact
    10021 gcacaaaagc atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg
    10081 gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc
    10141 aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct
    10201 cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct
    10261 ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta
    10321 ggcggatata tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg
    10381 atcacttcgt atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag
    10441 acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta
    10501 ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga
    10561 ttatggagtt cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa
    10621 aatatagaaa aatatagaag gaagctggac atttattgtt cccagcttct tttttgtata
    10681 gacctatttc tattttttta ttatattgga tatatatatt cattgaaaat agagcataac
    10741 aactttataa tgttgagtat accaattttt ggaggcgttt taatggaccg ctatttagat
    10801 ttactttcag agaagttccc tacaacggaa gatgtcatca cggaaatcat caatctcgaa
    10861 gcaattttgc agcttccaaa aggaacagag ttatttttga gcgatataca tggcgaattt
    10921 cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac
    10981 cttttctctg aacaaatgac ggaaacggag atcagccaat tcactatttt tacggcttat
    11041 ccagaatatg tgttgacgac tgattggtac aaaaaacaag ataaatccca gctgatccat
    11101 cgtttgatcg atcttttacg gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa
    11161 ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga tgatcgtatc
    11221 aacgggaaaa aagactatgt aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa
    11281 gaaaaatttt taacaaaact tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat
    11341 atcgtgggtg atatttttga tcgaggaact caagcagcca aagtaatgga tcgaatcatg
    11401 gatttttcct ctgtagatat ccagtggggg aatcatgata ttttgtggat tggtgcgtat
    11461 tgcggctctg aggcatgttt attgacgctt ttacggattg ccgcacgcta caactattta
    11521 tttgaattag agaaagaata tggattaaat ttacgtccat tattttcttt tgcccatcaa
    11581 acctatcaga agaatccagt atttacccct aaaaacagga aagatctatc tgaaagagag
    11641 caagaagaat tagaaaagat ccatcaagca ctttctatcc tccaattcaa atcagaacat
    11701 cagttgttga atcgacgacc tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc
    11761 aactatgagg aatcaacgat cgttttagaa ggtcagctct atgagttgaa agggacttgt
    11821 tttcaaacga tccagccaga tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc
    11881 gatagtttga tgtattcatt ccagcactcg ttaagaatgc aaaaacacat gacgtttttg
    11941 atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tactgttcca tggctgtata
    12001 cctgtagatg aaaaggggca gctcttagca tttgaattag accaatctta tcgcggaaaa
    12061 gaactgctgg gattttttga aaaacagata actgaaagcg caaaagattt gactgcaaat
    12121 gaagatttgg cgaccgattt gatttggtat gcgtggagcg ggccattttc gcctttattc
    12181 ggcaagagta aaatggcaac ttttgagcgc tattttctca cagagcctca tacgcacgtc
    12241 gaaaaaagca atccctatta tcacttgcga gatgaagagt ggttcagtga aaaaattttg
    12301 gcagaattcg atgcaaaaga agaaggttca gcaatcatca atggacacac gccagtaaaa
    12361 gtaaaaaaag gagagtcacc gatcaaagca aacggaaaag catttgttat tgacggaggt
    12421 ctatccaaag cttatcaaaa aacaaccggg attgctggct actcattact atgcaattct
    12481 tacggtttcc aaatcgtgac acattatccg ttcttgccta ttgaacaaca atttgcaaaa
    12541 aaatctgatc aaacgaatgt aaaaaaagtg atcgagcagc agctcccacg caaacttaat
    12601 cggaatacga caaaaggaat ggaattacag caacagatta ctcaactcca aaggctgttg
    12661 gattatcgaa aggacgattg agaaaaatgc tagccaacat tgctcaatag actaaaaagc
    12721 gactgcaaca aaaggcgtgt cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata
    12781 ttagaagcta tttttctgtt tatttctcta atactcaggt cagggcgctg atttcacgac
    12841 ctcttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccaacaa
    12901 acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac
    12961 gacctaatca gaaaaacgat taaaattcat tcacaaagaa atcgaataaa cgctgttcag
    13021 atgaatctac acgatgagaa agttccggat gccattggac accaaggatt ttgtgcatat
    13081 tatctttact ttggactgct tcaatgagtc catcgttgga aaaggcgatg gcttttaaca
    13141 aaggagaaag atctttgact gcctgatgat gataggaatt cacaaagtat gaatcaggga
    13201 gaaggtcact taataaactg tccgaaacga ttttgacttc gtgagttgca aactgcgggg
    13261 cagtgggctg ttgttcgtgt tttacggacc attttggata taatgaaaga tcttgataaa
    13321 gtgttccctc caacacgaca ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt
    13381 ttctttgttt caatgcttcc aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg
    13441 tttccgtcag ttttggatga gggtcttcca tgtaaagttg cggactaaca tcctgaccgc
    13501 ctgcaagaat gagtttgtcg attttctcaa ttagttgagg cgctgaatct ggagacatga
    13561 ctggcagaat aagaggtaat ccttgagcat ctaaaactgc atccacaaat ccctgcggtg
    13621 tatagcttac ttgatttcct tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa
    13681 tgccgatgat tggtttcatt tttattcccc ctaaagtaga ctaattgtaa caaggattta
    13741 aaaaaattta aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat
    13801 ctgtaaagag aattttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt
    13861 tataataaaa agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt
    13921 caaggggtga gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg
    13981 tacatacttt caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa
    14041 gcaagtgtta gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc
    14101 tcgaacccta cggcagcagt tgaagaccta tgcagaagaa aaagagcaag aattggagat
    14161 aatccaaacg aacagtcggc aacagctgaa acagttggta gaagaaaaag aacaattcga
    14221 aaagcaagaa ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc
    14281 aactttgaaa gaccaatatc aaaagcatca gagtcaagca atcggtgcaa tcattgaaag
    14341 ggtgaaagaa acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa
    14401 gaaatcagga gaggatttta caagcagtac aaggaatcca aaatgttgaa atccgggatc
    14461 tgtatcaaaa tagaacaaac aaccagtggg tagaaaaata ttttccagat accacgatta
    14521 ttgataaaga agcaaaatta agcgctttag gcaatcggct gaaagacatc caagaagcta
    14581 tccaattcat acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag
    14641 aactttcctt acaagacttg gaagcaaatt attccgaaga aaatttcatg aataaattag
    14701 aagaagtcct tgatttaaaa gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag
    14761 aagaagagga agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg
    14821 caagtaagac gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca
    14881 aggaagaact aagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac
    14941 aagtttatct ttcgtatgtt gctttaaaaa aatcagttgc tgctatagaa gaaattgctg
    15001 ggaaatacgg gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc
    15061 aagaaaccaa acagcaatta tccattgttc acgagcagca aaaaaagata acgacagcgt
    15121 taggtcagtg cagcggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg
    15181 ttgcagaacg ggaaaaagta aaagaacggt ttatccatgc cacttatctg atcgttttgc
    15241 aaggttgggt agatgcagaa gaaaaacaga atttgatgca tgtgttaaac gaaactgtat
    15301 cagaagaaga tatatacgtt agtttcgaaa caccaaactc agaagagatt cagcaggaag
    15361 tccctactaa attgaaaaac catccgctta tcgaaccttt tgaattgctg actgaaatgt
    15421 acagtctgcc aaaatatgaa gaagtagatc caacgccttg gatgacacca ttttatcttg
    15481 tcttcttcgg gatgatggtc gcagatgttg gttacggatt gctgatgctc attgggacaa
    15541 tactagctca aaaactattt gttttacccc gcgggatgaa acgtttcgta aaattctttg
    15601 agattctctc ggtaccatcg atcatctggg gattgatcta cagttcattt ttcggacagg
    15661 cattaccaaa agaactattt ggtatccgct tgcctttccc aatcctatct acgacggatg
    15721 acgtcaatac gattttgatt ttatccgtga ttttcggtct gatacagatc ttggttggat
    15781 tattcgtggc agcaaaggaa aatatcagga ggaaagatta cttgagtgct gtcagtgatg
    15841 gttttgcctg gcaagggatc ctgattggga tcgttattgc attgattggt gtcatgttga
    15901 taaaaaatac agcatttgtt tatcttggcg cggggctatc gatcattgct gctttatgta
    15961 tcttaatcgt gccgattatc caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt
    16021 ataatcttta cggattgact ggttatatcg gtgatttagt cagttataca cgtttgatgg
    16081 cactaggtat ttcaggcgga agtattggcg cggcattcaa tatgctagtt gctttcatgc
    16141 cgccagccgc tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata
    16201 tgtttttgac actgttaagt gcctatgtcc atggtgcacg attgcagtat gtcgaatttt
    16261 ttggaaaatt ttatacagga ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat
    16321 acgtcaacat caatcataag aaaaaaaaca ataaataatt ggaggatttt tatacaatga
    16381 tggattactt aattagtcaa aatggtggaa tggtatttgc agtattagcg atggcaacag
    16441 cgacgatttt ttcagggatt ggttcagcta aaggtgttgg aatgacaggt gaggcagcag
    16501 cagcgttaac aacaagtcaa cccgaaaaat tcggacaggc tttgattctg caattgcttc
    16561 caggtacgca agggttgtat ggattcgtta tcgccttttt gatttttatc aacttaggaa
    16621 atgatatgtc agttgtacaa ggattgaact ttctaggcgc ttctctgccg attgctttta
    16681 caggtttgtt ttcaggtatc gcccaaggga aagttgcagc agcaggtatc caaattttag
    16741 cgaaaaaacc tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg
    16801 cgattttagg tttcgttatt tccttcctat tagtattgaa tgcctaagca taaaggagga
    16861 aaagcagatg aacgccattg aaaaaatcat ctctcagatg aatgaagcag cagagcaaga
    16921 aagagcggcg ttagagcaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg
    16981 tacgcaaatc gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa
    17041 aaaataccgt cagcttagga atcgccaaca agtagaagta cgacaagaaa atttgaatgc
    17101 gaagcaggaa tttttacgac gattgtttgc agatgcaata gctgaaatgg agaattggga
    17161 tgaatctgaa caaatccagt ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa
    17221 agtaacattc attgctggag aaaaatcagc agcttatctt tctcagacat tgctggatga
    17281 atggaacaat gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc
    17341 gggttttctg ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca
    17401 agatatccaa ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat
    17461 cagtgtatgg aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg
    17521 tccaatgaga catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata
    17581 ttgaaatcca cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag
    17641 aatcttttaa aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct
    17701 gaaatcgttt ggatctatac gatgcgctat actttccata atctgaaggt tttgacaaaa
    17761 gccgagatga caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta
    17821 gaaaccttga aggatgccat tcatacgcaa gtttcaaatg aactccctga aagcattatg
    17881 gacagtatac gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac
    17941 gtgatttatg atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat
    18001 caagaattgc tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct
    18061 cgaggaatat tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga
    18121 agtatcgaaa aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat
    18181 tttctattag agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatctt
    18241 gatctcgtaa agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca
    18301 caaacccagg ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa
    18361 agcaaaaacc ttcgtttact ggttatcggc aaaagaagcc atttcgaacc agaaacaatc
    18421 agaaagcggg tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc
    18481 tgttttgcca ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt
    18541 tcgtcaggca attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca
    18601 atgtgcagca ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat
    18661 cgtattgatt cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa
    18721 cgtagaaaaa gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca
    18781 aattggaaaa atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc
    18841 cagtatccaa gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat
    18901 gcgtggcgat gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga
    18961 accggttcgc tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca
    19021 aatgtttgac ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt
    19081 cttagggcgc ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc
    19141 aacagtagaa gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac
    19201 aaaagttata aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat
    19261 cgaaaaaggt tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga
    19321 caaaaatttg acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa
    19381 attaaacccg gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt
    19441 gacaaaagga ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca
    19501 gcatcaaatt gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg
    19561 tggaaatgag atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg
    19621 agaatcgttg atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc
    19681 tcgagaagct tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta
    19741 tgacgttgct atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag
    19801 cggacgtttg gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct
    19861 tgcggaatac tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg
    19921 aagtataacc gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac
    19981 acaaaataca ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa
    20041 aagacatttt ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg
    20101 gaagtatatg gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg
    20161 tattttgcag gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct
    20221 ttcagataat gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca
    20281 gcaaaatgct tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt
    20341 gaagatgatc ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt
    20401 aagtgagatc atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat
    20461 cccggaaaca gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca
    20521 aacgatcgtt tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag
    20581 tcgtcggtcc attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg
    20641 aagtccgtat gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata
    20701 aagcaatggt ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac
    20761 gatttttagg ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg
    20821 atggtttggg acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata
    20881 ttaacggaga agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag
    20941 gtatctctgc aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt
    21001 ctggctctgg tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac
    21061 tcgattcatc agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag
    21121 ctgaattttt catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta
    21181 tgaatttagc aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg
    21241 ctgcagaata tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga
    21301 ccaactatgc agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa
    21361 gaggatatcc tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa
    21421 tccgcggact aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata
    21481 aaactcatcc aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta
    21541 gagagcttta caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt
    21601 taaaagataa gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc
    21661 agctttttgc agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg
    21721 aatctgcttt atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag
    21781 aatatgtcaa tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt
    21841 gggaactgct atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg
    21901 acaaatatct gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc
    21961 gcatggaatt gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat
    22021 taaaagataa gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc
    22081 agctgcgtca agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa
    22141 atgcttcgat cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg
    22201 atttgtctat cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat
    22261 atgatgaaca gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac
    22321 tggatcggtc aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa
    22381 tcgaaaaaac ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg
    22441 cattagaata tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc
    22501 tggaagaaaa cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca
    22561 ctgactaagt ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat
    22621 aaagaaacgt ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt
    22681 tggcggcggc ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat
    22741 cgatgcgctt ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac
    22801 agcagagcat tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg
    22861 tcttggtttt atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct
    22921 cagcacacgt attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa
    22981 gctcatgctt tacatcctaa aattggcctt tgttatccaa ttgattggta cggttgcttt
    23041 atcttttgta tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca
    23101 tgcaatctct agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca
    23161 ccagcaaaat gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg
    23221 cttcatcgta tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc
    23281 aaaaatcgca ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt
    23341 cacagagcgc aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac
    23401 cttttttatg agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat
    23461 gagccatgca ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc
    23521 gacggccggc ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat
    23581 gttgaaagga cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt
    23641 gcgggcactt actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt
    23701 atcggtgact gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt
    23761 ctctgcgttt ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg
    23821 gaaattgatc attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt
    23881 ctctctgatg acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt
    23941 catgattgga taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata
    24001 aaataatttt tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg
    24061 cactacccgt tgttgaacct cgggtagtgc ct
//
LOCUS       DAYYJC010000033        23884 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000033 DAYYJC010000000
VERSION     DAYYJC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.033"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACHA9N_001285"
     CDS             complement(54..788)
                     /locus_tag="ACHA9N_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9084755.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACHA9N_001286"
     CDS             1115..1525
                     /locus_tag="ACHA9N_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9084756.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACHA9N_001287"
     CDS             1518..2219
                     /locus_tag="ACHA9N_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9084757.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACHA9N_001288"
     CDS             2219..3805
                     /locus_tag="ACHA9N_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084758.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACHA9N_001289"
     CDS             3824..4045
                     /locus_tag="ACHA9N_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9084759.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACHA9N_001290"
     CDS             4042..4803
                     /locus_tag="ACHA9N_001290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9084760.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACHA9N_001291"
     CDS             5183..5692
                     /locus_tag="ACHA9N_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9084761.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACHA9N_001292"
     CDS             complement(5767..6306)
                     /locus_tag="ACHA9N_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9084762.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA9N_001293"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACHA9N_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9084763.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACHA9N_001294"
     CDS             7319..8170
                     /locus_tag="ACHA9N_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9084764.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACHA9N_001295"
     CDS             8198..8833
                     /locus_tag="ACHA9N_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9084765.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACHA9N_001296"
     CDS             8853..9626
                     /locus_tag="ACHA9N_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9084766.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACHA9N_001297"
     CDS             10034..10831
                     /locus_tag="ACHA9N_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9084767.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACHA9N_001298"
     CDS             10809..11222
                     /locus_tag="ACHA9N_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9084768.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA9N_001299"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACHA9N_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9084769.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA9N_001300"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACHA9N_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9084770.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACHA9N_001301"
     CDS             15999..16559
                     /locus_tag="ACHA9N_001301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9084771.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA9N_001302"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACHA9N_001302"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9084772.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACHA9N_001303"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACHA9N_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9084773.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA9N_001304"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACHA9N_001304"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9084774.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHA9N_001305"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACHA9N_001305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9084775.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHA9N_001306"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACHA9N_001306"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9084776.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACHA9N_001307"
     CDS             21906..22484
                     /locus_tag="ACHA9N_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9084777.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACHA9N_001308"
     CDS             22536..23018
                     /locus_tag="ACHA9N_001308"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9084778.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACHA9N_001309"
     CDS             23135..23509
                     /locus_tag="ACHA9N_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084779.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4289 c         5123 g         6663 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtc
//
LOCUS       DAYYJC010000034        23726 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000034 DAYYJC010000000
VERSION     DAYYJC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.034"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..523
                     /locus_tag="ACHA9N_001310"
     CDS             122..523
                     /locus_tag="ACHA9N_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9084780.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            517..762
                     /locus_tag="ACHA9N_001311"
     CDS             517..762
                     /locus_tag="ACHA9N_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084781.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1086..1307)
                     /locus_tag="ACHA9N_001312"
     CDS             complement(1086..1307)
                     /locus_tag="ACHA9N_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084782.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1469..1541)
                     /locus_tag="ACHA9N_001313"
     tRNA            complement(1469..1541)
                     /locus_tag="ACHA9N_001313"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1506..1508),aa:Lys,seq:ctt)
     gene            1617..1847
                     /locus_tag="ACHA9N_001314"
     CDS             1617..1847
                     /locus_tag="ACHA9N_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084783.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1891..3060
                     /locus_tag="ACHA9N_001315"
     CDS             1891..3060
                     /locus_tag="ACHA9N_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084784.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            3634..4203
                     /locus_tag="ACHA9N_001316"
     CDS             3634..4203
                     /locus_tag="ACHA9N_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9084785.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4190..5050
                     /locus_tag="ACHA9N_001317"
     CDS             4190..5050
                     /locus_tag="ACHA9N_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9084786.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5240..5944
                     /gene="yycF"
                     /locus_tag="ACHA9N_001318"
     CDS             5240..5944
                     /gene="yycF"
                     /locus_tag="ACHA9N_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9084787.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5949..7784
                     /gene="walK"
                     /locus_tag="ACHA9N_001319"
     CDS             5949..7784
                     /gene="walK"
                     /locus_tag="ACHA9N_001319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9084788.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7781..9097
                     /gene="yycH"
                     /locus_tag="ACHA9N_001320"
     CDS             7781..9097
                     /gene="yycH"
                     /locus_tag="ACHA9N_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9084789.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            9098..9967
                     /locus_tag="ACHA9N_001321"
     CDS             9098..9967
                     /locus_tag="ACHA9N_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9084790.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            10022..10831
                     /locus_tag="ACHA9N_001322"
     CDS             10022..10831
                     /locus_tag="ACHA9N_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9084791.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10934..11563)
                     /locus_tag="ACHA9N_001323"
     CDS             complement(10934..11563)
                     /locus_tag="ACHA9N_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9084792.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11733..13025
                     /locus_tag="ACHA9N_001324"
     CDS             11733..13025
                     /locus_tag="ACHA9N_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9084793.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(13101..13763)
                     /locus_tag="ACHA9N_001325"
     CDS             complement(13101..13763)
                     /locus_tag="ACHA9N_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9084794.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13988..14272
                     /gene="groES"
                     /locus_tag="ACHA9N_001326"
     CDS             13988..14272
                     /gene="groES"
                     /locus_tag="ACHA9N_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9084795.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14323..15948
                     /gene="groL"
                     /locus_tag="ACHA9N_001327"
     CDS             14323..15948
                     /gene="groL"
                     /locus_tag="ACHA9N_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9084796.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(16037..17176)
                     /locus_tag="ACHA9N_001328"
     CDS             complement(16037..17176)
                     /locus_tag="ACHA9N_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9084797.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(17287..17871)
                     /locus_tag="ACHA9N_001329"
     CDS             complement(17287..17871)
                     /locus_tag="ACHA9N_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9084798.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17998..18429)
                     /locus_tag="ACHA9N_001330"
     CDS             complement(17998..18429)
                     /locus_tag="ACHA9N_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9084799.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(18447..18767)
                     /locus_tag="ACHA9N_001331"
     CDS             complement(18447..18767)
                     /locus_tag="ACHA9N_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9084800.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            19067..19207
                     /locus_tag="ACHA9N_001332"
     CDS             19067..19207
                     /locus_tag="ACHA9N_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084801.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            19505..19762
                     /locus_tag="ACHA9N_001333"
     CDS             19505..19762
                     /locus_tag="ACHA9N_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFM9084802.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(19733..20041)
                     /locus_tag="ACHA9N_001334"
     CDS             complement(19733..20041)
                     /locus_tag="ACHA9N_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084803.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            20119..20865
                     /locus_tag="ACHA9N_001335"
     CDS             20119..20865
                     /locus_tag="ACHA9N_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9084804.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            20880..21083
                     /locus_tag="ACHA9N_001336"
     CDS             20880..21083
                     /locus_tag="ACHA9N_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084805.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            21188..21487
                     /locus_tag="ACHA9N_001337"
     CDS             21188..21487
                     /locus_tag="ACHA9N_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084806.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            21487..21669
                     /locus_tag="ACHA9N_001338"
     CDS             21487..21669
                     /locus_tag="ACHA9N_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084807.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(21675..22151)
                     /locus_tag="ACHA9N_001339"
     CDS             complement(21675..22151)
                     /locus_tag="ACHA9N_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084808.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            22204..22929
                     /locus_tag="ACHA9N_001340"
     CDS             22204..22929
                     /locus_tag="ACHA9N_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9084809.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            23103..23444
                     /locus_tag="ACHA9N_001341"
     CDS             23103..23444
                     /locus_tag="ACHA9N_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084810.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            23437..>23726
                     /locus_tag="ACHA9N_001342"
     CDS             23437..>23726
                     /locus_tag="ACHA9N_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9084811.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         8127 a         3894 c         4678 g         7027 t
ORIGIN      
        1 tccttttttt tataaaaaga ttttctgttt aaagaaggat atttttttct agttttttcg
       61 aaaaaaaggc gctttctgat tgttgtatta gtggaaaggg gaaaaaagga gcatatttat
      121 catgcaacaa aattacaatg agaaatctat acaacatcaa tttgatgcct actgtaaaaa
      181 agtattacga aacgaaacta agtctattcg aaaacgaaac attaacataa cagataatga
      241 ggaaccattg acgaattcaa atgaaggtaa gtactcccaa actcattttg atgaacaaga
      301 tgtctatttc ttatttggaa tggaaatatt aatttcagat caaaagttat cggcagccat
      361 tgatcattta tcggaaataa gaagaaaggt tatcctactt tattattttg caggttttaa
      421 tgatactgaa atcggtaaaa tcctcaatat gagtacaagt ggtatctggt atcaacgaaa
      481 gaaggcagtt gaacaattaa aaatggggta tgacttatgg tagacaaccc caaaaaacgg
      541 cagtatccat tagttcctct atccattatc caatctgctc tagaagaaga tccaatttca
      601 atcaagatga tactcaaaca ttatcatagt tatatagcta agttatgttt gacaaatgga
      661 tttaatgagg cggggcaatt tattacctat gtagatgagt atatgctaag acagctagaa
      721 attaaattga tagaagcgat cttaaaattt aaaattaact aactaaaaag tcccctttcc
      781 acttattctg tttaaaagaa tcttctaata gttaaaggat tagtagattc ttttaaacag
      841 aaaacctatt gatcattgaa atttaaatac attctatcag tacgttaaca cttcaaggag
      901 catattttag ttgcgccatg atttcttact gtaaagaagg agcgataaac attctaaaat
      961 aatttttata gtcaataaaa agcgatgatt tgaagttggg ataatgatac ttccgtaagg
     1021 ctcgccgtaa acttggagag aggtgaaatt cctatgtgct tttgacaaaa gcagctgatt
     1081 aagaattatt tccatatgtt gatttcaaca ttttttctat tatttttaag accaaattca
     1141 gtgaggcaaa tagcatcatg taaatttagt ttttctattg aacgttctcc actttttagt
     1201 ttttttatag tactcaaatt taatcctgta tgtttagaga tctcatttgc agtatggttt
     1261 tgtaaagttt ctcggattat tgttatatta gcaatttcat tacgcataat tatgttcctt
     1321 tcttatcaaa aatatcaatg actacagtgg gcttttagtg aaaaaaaggg gtactacagt
     1381 ctcttttgaa aacaaaaaaa tacagtatat acgttttcta aatgctaagc accaacgttt
     1441 acactgtatt taagggtttg gcacccaatg ggctgtgagg ggctcgaacc cgcgacccga
     1501 tgattaagag tcatctgctc taccaactga gctaacagcc caaattaata ctttgttatt
     1561 ctaacaccta aaataaaaaa gtgcaatact tattatcaga attgagagtg aattttatgg
     1621 attttcaaga ttatgaaaaa atatttgaaa gttatccatc gatattgaaa attactgatt
     1681 taactaaaat gttaaatatt ggaaaaaata aagcttataa tttgctgaaa gatagatcaa
     1741 tagaaaatat aaaaattgga gcaaggtaca aaatacctaa aaaagctgtc attgagtata
     1801 tattgaaaat acaagatgaa ggtaaaaatc atgctataat aaaatagtca aaatttttgc
     1861 cgatatttca gtttaagagg agagattgaa ttgaaattaa gagcaaacat aattgaaaaa
     1921 agaggaacat tacaagtagt tgtatcttat aaagactcta acggaaaaat tagacaaaag
     1981 tggaaagata caaaattaaa aaagaaagga aataaaaaac gagcagaaaa aattagagat
     2041 gaatttttac tagaattgga aagtcaatta gctatggaat gtgaatgtgc taatccaaat
     2101 ttattgtttt atgactattt gcttaattat ttggaagtat caaaaaaaca agtagcattt
     2161 aacacatatg ttgggtataa gcattacgtc cataatcgaa tttacaagtt tttttatcct
     2221 aaaaaaatta aactaaatag gttaaagcct tatcatttac aagagtttta tcaatatatg
     2281 ttaaatgata actgtacagc aaatacggtt attcattatc acgcatttat acgcaaagct
     2341 cttcaagagg ctgttattac agaattgata agtacaaatg tagcagataa agtaagaaag
     2401 ccaaaaaaga aacaatttgt ttctcaagtt tataatcaac aagagatctt gaaattactt
     2461 gacattttgt cagaagaaaa actctatttg gtagttcttt taacagcatt ttatggattg
     2521 cgaagaagtg aagttttagg attaaaatgg tctgctattg attttttaaa caaaaaaatc
     2581 atgataaacc atgtaatcat agaaaatcca gagaatatgt cacagttaat aaagaaagat
     2641 caaacaaaga actcttccag ctatcgtaca ttacctttgg ttgaaacgat tgaacgagca
     2701 ttagtaaatc aagctaaatg gcaacaagat aatcgtattc ttttaggaga tgattatatt
     2761 ttagaagata atgagtacgt atttactatg gaagatggac ggttgatgaa gcctcaatat
     2821 gtaacgcatc gtttgagtaa gttgattaag aaaaatggat tgaaaaaaat tcgctttcat
     2881 gacttgcgtc attctaatgc ttcaataatg ttagatagtg gccagaatat gaaatccatt
     2941 caagaatggc tgggacatgc atcgtattcg acaactgcta atttatacac gcatttaacc
     3001 gcaggagtaa aagatcgtgc agcagaagca ttagaaaacg catttgggtt taaagaataa
     3061 aaaaacaaaa aacaatatct ttggggtatc gcttcttgtt tttagaattt ttttgttggc
     3121 aatttgttgg cagtatgttg gcaatgaaaa aattcacaaa ctaaaaacct atttataaag
     3181 ctttataaaa cttttttcac aaatagagca tttggttaag agtcagctgc tctaccaact
     3241 gagctaacgg cccgtacaaa aagtacttaa taatagtagc actaagaatt ttaaaatgca
     3301 agactacatt taaagaaaat cagaaaaatc tttacttcct tataggaaat cattcataga
     3361 ttattcacat ttgctcgcta ttatataagc ataccaacaa acaaagttct tgcaagagaa
     3421 caaaccctaa cacttttttc attttttact cctttcccgc cgtctccccg gcgggtctcc
     3481 tgttcttaac gataaaagac aagaaggctt agccttcttg ttttttgttt tcatggaatc
     3541 ccgctaatca aatcacctag ttaaagcaac tagattgaat gtttatcata agtctgatat
     3601 catgtttatg aaatgaaaaa aggagcggtt atgatggaaa aaatgcgtca gcaattaatg
     3661 gagtggggga aggaactaga aacattccgt ctgcctcact gggaagaact ccctgactta
     3721 gaactttata tggaccaagt cagaacgtta gttgatcgtt atctttcacc tgtgatacaa
     3781 ggagaaaagc atccattgct tacctcttct atggtaaaca attacgttaa actaggtttg
     3841 ataccagcac cagttaaaaa aagatataac aaagaacacg tagcattttt acttgcaatc
     3901 actacgttga aacaagtatt gactattcct gaaatcaaag aagggatatt gttccaagga
     3961 aaaacagtag gaattcgtga agcctataat ctattttgcg atgaacaaga agcagctgtg
     4021 tggatggtca gccaattagc acaaggtaaa tctcatccgc aaaaattcga ccaggcaacc
     4081 cctgtagaat atattgctgt aagagcggca actttatcgt ttgcaatgaa acttcttgcg
     4141 gaaaaaacga ttgttctaga aaccgaatat ttaaaagagg agaaaacaaa tgaaaaacaa
     4201 taaaatcgca attttagtag attcaggtac ggatgttcca gaagaattga taaaaaaata
     4261 cgagatgtat atgatcccat tgaaaattat ttataaagat caggtatata cagataaaat
     4321 tgacattacc cctgaagaga tctatcaacg tttgccagag gaaatccctg gtacatcttt
     4381 gccggatggt gagtcgatca cagaaatctt tgagcaaatc aaaaaagacg gttatgaaaa
     4441 ggtacttgcg gtaacaattt ccagtggatt gagcgggaca tataatgtgg ttcgtttaat
     4501 tgcacaacag caagaagaat tggaagcata tgtactagat acgaaaaata ttggtattgg
     4561 tgcaggtttt tctgcgatcc aagcagcaaa atggctggaa gaaggagtag aatgggatca
     4621 actgatcagt aatctgaatg agttagtcga aagaacaaaa gttttcttta atgttgcgac
     4681 attggaatat cttcaaaaag gtggacggat cggtttagtt gcttctattg taggaaccgc
     4741 tttaaagctt aacccaatca tctcatgtaa tgaagaaggg atttattaca cagtaggaaa
     4801 agctagggga cgtaaaaaaa gtttggataa aacggttgct tacgtcaaag aaaaaatcgg
     4861 taatgcaaag gtcttcaact tagctgttgc ccatggggat gcgaaagaag aagcaattga
     4921 aatggaagcg agacttaaaa gagagttccc acaagcaaac caaatctact ttggccagat
     4981 ttctccggca ttagtagtcc acactggtcc gggattattg ggtgtcggtg tacaagtttt
     5041 agaaggataa atcaaaaaaa ggaaacactg aggtgaaaat cagtgttttc cttttttatt
     5101 gaagttagaa caaaaaataa taaagtattt tgcactttca ttttaatttt ctcttttgtt
     5161 ttataggcaa agatgaaaca aaagcagaaa tacggtataa tgagaaggta tgacaataat
     5221 atataagagg tggctcttta tgaaaaaagt tttagttgtc gatgatgaga agccgatttc
     5281 ggatatcgtc aaattcaatt tagccaaaga aggctatgac gtttatactg catatgatgg
     5341 ggaagaagcc ttggaaaaag ttgctgaggt agaacctgat ttgattttac ttgatttgat
     5401 gctacctaaa atggacggct tagaagttgc tcgtgaggta cgcaaaactt atgatatgcc
     5461 gatcattatg gtaacagcca aagattctga aattgataaa gtcctaggct tagagttagg
     5521 cgccgatgat tacgtgacca agccattttc caaccgagaa ttagttgcac gtgtcaaagc
     5581 aaatcttcgt cgcggtgcca cggctgcgaa ggaaccagaa gaggcagcac ctgcggaatt
     5641 gacgattggt gatttgacga tccatccaga agcttacatg gtgacgaaac gaggagaaac
     5701 aatcgagtta acacatcgtg aattcgaact actcttttac ttggcgaagc accttggaca
     5761 agtgatgaca agagaacatt tattacaaac ggtttggggt tatgattatt ttggagatgt
     5821 acgaacagtg gatgtaactg tacgtcgttt gcgtgaaaag atcgaagata atccaagcca
     5881 tccaaattat ttagtgacac gtcgtggtgt cggttactat ttgcgtaacc cagaacagga
     5941 gtaattggat gaaaagaaaa gtccgctttt ttcgatcagt caattttaag atcgctatta
     6001 cctttattct gattttattg atctcgatcg aaattattgg tgcctacttt atacgtggtt
     6061 tagaacgttc cacgatcaat acatttatta aagatatgaa tcaaacggtg gaatcattag
     6121 caacaacaat cagcccggaa ttgaatcgaa aagataatgc agatgatgaa gaggtaaatg
     6181 cgaatatcaa aagatttatt gaaaacagtg cgactagcga tatcatcgaa atacgggtcg
     6241 ttgacgaaaa agggatcatc cgaggaacaa ccgatgtcaa tgaacaaagc gctgtgggaa
     6301 agaaaaatga ttatgttgat atcaatgatt tcacgactaa acgttatgtt gctttagata
     6361 acgacgataa acgggtgaat atcaatgttc aaccgatcct gtccccgacc ggcgatgccg
     6421 ttatcggggc tctttacgtc aaaagtaata tcgaacaaaa atattcggaa atcaataaca
     6481 cagcagtcat tttctttact gcctcactaa ttgcggcatt tatttcaatg atcgtatctg
     6541 ttttagttgc tcgttcgatt acacagccga tcggtgaaat gagagaacaa gccattcgaa
     6601 tcgctagagg agactacagt cggaaagtca aagtgcatgg acaagatgaa cttggacagc
     6661 tggctgatac cttcaatcag ttagctgaac gtattgaaga agctcaagat acaatggaag
     6721 ctgaacgcaa tcgtttggat agcgtgctat cccatatgac agacggcgtc attgcgacag
     6781 atcgcagggg taaagtcatt acgatcaatg atatggctgt ttccttgcta gatatcaaaa
     6841 acgaggaagc aatcggacaa tcgattttga cacttttaga tatcgaagaa gaatatacgt
     6901 tgcggaaatt attagaagat ccaaacgaaa tggtacttga ccgttcaacc agtacattgg
     6961 aaagtgatca gatgatcttg cgagtagact tttctatgat ccgtcgcgaa tcaggtttta
     7021 tcagcggatt agtagctgtc ttgcatgatg tgacagaaca agaaaaaaca gaacgggaac
     7081 gtcgtgaatt cgtctctaat gtttcccatg aacttcgtac accgttgaca agtatgcgta
     7141 gttatatcga agcattgagc gaaggtgcgt ggaaagacga ggaagtagca ccaaattttt
     7201 tgaaggtaac gctagatgaa acagaccgaa tgattcgaat gatcaatgac ctcttaaatc
     7261 tatctcgcat ggatacaggt aatacccagc ttcaattaga atatgtcaat ttcaacgagt
     7321 tagtgaattt tgttcttgac cggtttgata tgatggtggg aaatcaggaa aaaaattata
     7381 agatccgtcg tgaatttacg caaagagatc tttgggtcga agtagataca gataaaatca
     7441 tccaagtagt cgataatatc atgaataacg cgatcaagta ttcaccagat gggggaacga
     7501 ttacttgtcg cttattagaa acgcataata acgtgatttt aagtatcacc gatcaaggat
     7561 tggggattcc taaaaaagat ctgaaccgag tgttcgagcg gttctatcga gtagataaag
     7621 cacgtgcaag agcacaaggt gggacaggac ttggattagc tatttctaga gaagtgatta
     7681 aggcgcatcg aggcgctatt tgggcagaga gcaaggaagg aaaagggtcg actttttata
     7741 tctcgctgcc atatgaacct tatgaggagg aatggtggga atgagaatct ctgaaaaagt
     7801 cgttcgtata ggactcattt tgatggttgc gttaagcata tacttctctt atgcaatctg
     7861 gctgagccca gcaggaaaaa gttcaatcaa cgtagatgag acaaacactc aaatgatcga
     7921 gtctcaaagc aatcgaaaaa cttccgaagt ctttcttccg cttcatgtta catggcacca
     7981 ttcgggaatg atccaagaaa ccaacagtga aaatctcgtg taccgattgc aattgatcat
     8041 cgataaagca agatttggaa agctttctga agtagtttca ggagacaaat cacagtttga
     8101 acaggtgaaa aatctgaact ctggttttga gttggcctat aatgcgcctt ttttgctaag
     8161 agaatacaag gaagcatttg atttagattt agatgtgtcg aacttggcaa aagaaagcga
     8221 aaatatgtat tttactagaa tacaattcga tcaaggaaat aaaaaaatcc gatttttgaa
     8281 ttattcgaac catctcgtat atgaagcaaa tatttcgttg aattggacag atatcaataa
     8341 agaattaaca gcaaaagaca caaaatggac agaaatgaca acagaaccat cgacgatcga
     8401 cacgcaatat gctacgaaac attcgatcaa actgaagaag tatagttata tcctgtcaca
     8461 gcaaccctat acacttttta gaaatgcttt tttccagaaa ccagatagcg tgaaaaataa
     8521 tgatgaaagc aatgaattga ttttttacgg tgggaatgaa acaatgtcta tccattcgga
     8581 aacgcaagta gttgattttc gtggagagct gccagagaaa aaagaaaatg ctagtgtatt
     8641 ttctgaaagt ttcccctata ttagcaaatt aggcagttct ttagggaacc tcagatattt
     8701 tgaccgcagt gacaatacga tcgattatcg gatattcgtg gaaggcttcc cggtttttgg
     8761 gacagacagc agaggacaga ttggtgtgga agttggcggt gaaacgaatg gttctgtgca
     8821 agtgaatatc caaaccagtt tgaatacgat tcaagtgccg ataccttcag acgaagaagt
     8881 agaacttgca agtacgaatg aaatcatcca gcagttaaaa gctaagggag cagatagcaa
     8941 caagattggt tcagtgatcg ttggctatac atggcaaaat gtcaaagaaa cgaatcgtgt
     9001 cgttgatctc ttacctgaat ggtatgtgaa atataaagat aactggtatt cagctaacga
     9061 ccttctggaa aaattacctg gattggaggc gaattaattg gatttcaaaa aaatcgaatg
     9121 gatatttttc gtcgcttttt tagggttgaa tgtgttttta ttcagcagtt atcatgacgc
     9181 ggtataccaa gaaaacaatg tcattcgctc taatcagact gaatcaatcc aacaacgtct
     9241 ggctagcgac gatatcactt acacggggaa gatttcgtct gagaacaagc aaggctatta
     9301 tcttagcgcg gagcagacca atcttagcac ttcattagcg aattatcgca aggctggtaa
     9361 aagcggcttg ctggcaagtg gtgtgttttt agacggtgat gttttgacga aatctttatc
     9421 cagttcagat gctgaaaaga acgtgatcga tccagataaa attagtagtt ccttggaaga
     9481 ttttttatct aacaaagaca atattttatt cggtaatgat tatgtgtaca cgaaaaactt
     9541 ttctcattta gaagaagaac cactagaaat ccaagcaagc caagagtatg aaggcatccc
     9601 attcaatgat gatacagcaa aaatcgttct ttctttagaa aaaaacgata atgattatag
     9661 catcacgaaa tacatgcaga cgcatttatc tgatatcgag cagctgcgcg aaaagacgga
     9721 actgtatacg gaagaggaag caatcaatac attgtacatc aataacaaaa taccaagagg
     9781 atcaaaaatt ttatggcgtc agctagctta ttcctgtatt ctgaaagtcc gggaaaaaaa
     9841 tgtgtatgtt cctgtttggc atgtagcgat cgaaacaagc gataaagtgg tccaaattga
     9901 gagcgtcaac gctttcagca atacgatcgt gacaaacaat actgtaccaa aagtggaaga
     9961 tcaataaaaa agcgagtata atgaaaaaag tttacaaagt aagaagggaa gtaggagtca
    10021 aatggctgaa gaaaaaccag cttttaaaat cagtatccta gcaagtggaa gttcgggtaa
    10081 ttcactttat atcgaaagtg aaaaaaaacg gcttttggtc gatgcaggac ttagcggtaa
    10141 aaaaatcact tccttgatgg ctcaaattga tcggacaccg gaccaattag atggaatact
    10201 tgtgacgcat gaacatcgag atcatataca tggcgtgggt gtcttggctc gtaagtatca
    10261 tttggatgtt tttgccaatc agcagacttg ggaagcgatg gaaccactga ttggaaatgt
    10321 accagtggaa caaaaacatc tatttgagat ggggaaagtc agaacattcg gcgatatcga
    10381 tattgagagt ttcggcgtct cccatgacgc agcggctccg caattttatc gtttccataa
    10441 agaaaatcgg tcattcgtca tgttgacgga caccggatat tgcagtgatt atatgcgagg
    10501 tgtcattgaa aatgccgatg gttatttgat ggaaagtaat catgatcttg agatgttacg
    10561 catgggacct tatccgtgga atttaaaaca gcgtatttta ggagatagag gacatctttc
    10621 caacgaagac ggtgcacttg tcatgactga tgtgatcggt aatcggacca aacgaatcta
    10681 tcttggccat cttagcaagg aaaacaatat gaaagaactt gctcatttaa caatggaaaa
    10741 tgttttgaaa gaaaaagatt ttggcgttgg acatgatttt gaaatctatg atacagatcc
    10801 agatgaagca actgagctat tttcgattta aacagttcaa tcaataaaaa tataaaaagc
    10861 agaagataaa atcaatcagg gttggtttta tcttctattt ttattttttt agcatattaa
    10921 aaaaataacc gtattatcga ctgactacac tgatgtgctg cttttcatgt ttctcatcca
    10981 gtatctcatc tactaatgca atcgcgtaat ctgccatgct gatcgtactg tcgctagcag
    11041 cgtttctttg taaataatca tcacttaaca aataagtacc tgtacgtttg ccatgtggat
    11101 caaataaaga agctggactg atataagtcc aatgtagatt tttagattgt ttcaatttta
    11161 acaatccttc gtacatattg aaagctgttg gatagaaggg tgcattttta ggtacttggt
    11221 caatcatacg agtcgtcttt tctgtatcta agaataacga agaggcaccc ccaacgacaa
    11281 ttaagcgtgt atcggttcct tcaagcatct caatcaaatg ggacaaagaa gtttgatgca
    11341 tttcttcttt tcctttggga gcgttgaaag catcaacgac aacatcaaaa gatgcaagat
    11401 cagtagttgt caactcaaat aaatcctttt gtataaacgg aacatctgat ggcaattttt
    11461 ctggatgacg cacgattgcc gtcacgtcta aatctctgga caatgcttcg tctaaaataa
    11521 atgaacctgc atggccagta gctccaatga gtgcaatttt cataaaaaac ctcctttttt
    11581 attcctgtcc attgtaagca gttgctgaat attcgtcaaa gaatccgatt tttgaaaagc
    11641 tcagtgcgca ttcctttcat ggttcgtgat cattgggtat agttaaatta agaaaactga
    11701 ttgataaaaa tagaagttga aggagataag aaatgaaaga gtggttagat cgtgcctttt
    11761 taccaatgat aacatctgtt gtgaatctta gacccttaac tgctttgcgt aatggaatga
    11821 cttacacgat cccaataatt attgttggct cgctgttttt cttatttgct cattttcctg
    11881 tggattacat cgctcagtgg atagacaaaa caggattttc tatgtatttt acacaagtat
    11941 accaggctac atttgatgtg atagcctttt ggatcgtttt tgccattgca tacgcttatg
    12001 caaaagaaga agacatcgat cgcttaccag tcggcataac agccttgtct gcttatctac
    12061 tgcttattca gccactaaat actcagatag atactgtctg gacaggtgct gaagggatac
    12121 taggagcagt tacaactggg ctcatcatcg gatggggcta tgcacgttta gtaagacttg
    12181 cgaaagggtg gaagcaaaga gagaagatac ctgaaaatgt ttggcagtct tttacttcat
    12241 tgattccaat cattgttata gtcactttta cgcttatcgg ttcagcggtt tttcaacatt
    12301 tgttcagaat gactgttgtt caaggagtat ggacatatgt ccagcttccc attcaatcgt
    12361 tcatagacac gttgtttggt gtgcttttat taggtttttt tgttccgttt ttatggttat
    12421 ttggtcttca tggatctgcg atggtcaatg ggttggttag tcccgtcttg caagcgaatt
    12481 cattagcaaa tgcagaaatc ttagcctcag gacaagagtt gacggtggca aatggcggac
    12541 atatcgttac tcagcaattt ctcgatcagt tcatgacggt tacgggtgct ggattgacgt
    12601 taggggctgt attttttatg acggtttttg cgaaatcgaa gaaatacaga gaattaggaa
    12661 atcttgctct tttaccagcc tttttcaaca tcaatgaatc aattattttt tccacaccga
    12721 tcgtgatgaa cccgatgatg gctgttcctt ttattttcgc accggtcgtt tcaggattga
    12781 ttacctattc cgctttatat tttggattcg ttcctttatt tacagcagtc caagtccctt
    12841 ggacaacacc gccgatcctc tcgggtttct tagtaggcgg agtgcctgca gcgattttac
    12901 aaggtatcgt gttgtcaatt agttttttca tttatttccc atttttgaaa aaaatagatt
    12961 ctgcaaactg caaaaaggaa agacagacaa aaaatgatgg agcagcagaa aagatgaacg
    13021 actaaaaaac aaaaatactc ctgtcgcttt agattgggaa aatcaccaat ctaggatgac
    13081 aggagtattt atagtcatgt ttatactagc cctttttgga cagctaattg aacaagaaca
    13141 actaatgtgt tcattcctac gtgtgtgatc atcgatgtcc agatccgacc agttgctttg
    13201 tactctaggc tgaagaagaa acctaggaaa aagtagatta gcagatgacc atcattatga
    13261 gcaaacgcaa acagcaaaga gctgatcact gctccgaccc aaaaatttga atatcgactg
    13321 atgatcccca gtatactgcg gcggaatacg aactcttcca tgatgggtcc agcaacagtt
    13381 gtcgccaaaa taaaagcggg tgcttccatg atcatctgga tgatattttg tgtattctcg
    13441 gaaggggttg ctttgccaaa caatacagat tctaacatga cagcgatgct ttgcagaatc
    13501 aatgcgacaa agattccaac caatccataa ataacaatct ttggagcaga agcttttttt
    13561 accgctgatt cgatcgctaa gggttcacgt tgcttaaaat aaaaaatagc taggacaacg
    13621 gcgcctagta tatacatgac tgcagttgct gtgatcaatt cagacgtaga ttttacaagt
    13681 cctatcgaag ctaggatcaa gggtgagata aaaattagac catagcaaaa aatgctgagg
    13741 aagctatatt tttttaaaga catgtaattc ctccttttaa gggataacaa gcatgcaagg
    13801 caagcttata tcgattgatt tcaattatat cataatttca ataagcagct gtcagaataa
    13861 aatttctttt ctttgattta aaagcttgca aaaaagaggg agaatgagta ttattaaaaa
    13921 tgtaggttag cactcgaagg ttaagagtgc taacagatta aaaaaagagt tatcggaggg
    13981 atttatcgtg ttaaaaccat taggtgatcg cgtcattatc gaagtcgcaa aagaagaaga
    14041 aaaaactgtc ggaggaatcg ttttagcttc tgctgctaaa gagaaacccc aaactgggac
    14101 agttgttgca gtcggtgaag gccgtttgtt agaaaacggt gaaaaagttc ctgccgctgt
    14161 aaaagcaggc gaccaagtta tgttcgaaaa atacgccggt acagaagtaa aatacgaagg
    14221 aaaagaatat ctgatcgtcg caggaaaaga tatcatggcg attgttgagt aaatttggaa
    14281 gataaaaaaa taatcaaaaa taatcaatga ggtgaatcaa tcatggcaaa agaattgaaa
    14341 tttgcagaag acgcacgcgc agctatgtta cgcggggtag ataaattagc agatacagtg
    14401 aaagtaacat taggacctaa aggccgaaat gtcgtactag aaaaatctta cggctctcca
    14461 ttgatcacaa atgatggtgt gacgatcgca aaagaaatcg aattagaaga tcattttgaa
    14521 aacatgggtg caaaacttgt atcagaagta gcatcaaaaa caaatgatat tgcaggagac
    14581 ggtacaacga cagcaactgt tctgacacaa gcaatcgttc gcgaaggttt aaaaaacgta
    14641 actgcaggtg ctaatcctct aggcattcgt cgcgggatcg aattggcaac aaaagcagca
    14701 gtcgaagaat tgcataatat ctcaactgtt gttgattcaa aagaagccat tgcacaagta
    14761 gcagctgttt cttcgggttc tgataaagtt ggacatttga ttgctgatgc aatggaaaaa
    14821 gttggaaacg acggtgtcat cacaatcgaa gaatcaaaag ggatcgaaac agaactagat
    14881 gttgtagaag gtatgcagtt tgatcgtggt tacttatcac aatatatggt aacagataac
    14941 gacaaaatgg aagctgttct agaaaaccca tatatcttga tcacagacaa aaaaatctct
    15001 aacatccaag atattctgcc attgttagaa caaatcttgc aacaatcacg tccattgttg
    15061 attattgctg acgatgtaga tggtgaagca ttgccaactc ttgtattgaa caaaatccgt
    15121 ggaacattca acgtagtagc tgtaaaagct cctggtttcg gcgatcgtcg taaagcaatg
    15181 cttgaagata tcgctatttt gacaggtggt acagtaatta ctgacgatct aggtttagaa
    15241 ttgaaagatg caacaatcga aaacttagga aatgcttcta aagttgttgt agataaagac
    15301 aatacaacga tcgtagaagg ttctggagaa aaagaagcaa tcgaagctcg tgtgcaattg
    15361 atcaaaaacc aaattgctga aacaacttct gattttgacc gcgaaaaatt acaagaacgc
    15421 ttagctaaat tagcaggtgg tgtagctgtt gttaaagttg gtgcagctac tgaaacagaa
    15481 ttgaaagaat taaaattacg cattgaagat gcattgaatg ctacacgtgc cgcagtagaa
    15541 gaaggaatgg tctcaggtgg tggtacagct ctagtgaacg ttatcagcaa agtttctgct
    15601 gtagaagcag aaggagacgt ggcaacaggt atcaagatcg ttgttcgtgc tttagaagaa
    15661 ccaattcgtc aaatcgctga aaacgctggt tatgaaggat cagtgattgt tgataaattg
    15721 aagaacgtgg aactaggcac tggtttcaat gctgctactg gagaatgggt aaacatggtt
    15781 gaagcaggta tcgtagaccc aactaaagta acacgttctg ccttgcaaaa tgccgcttcc
    15841 gtttctgcct tgttattaac gacagaagca gtcgttgcag ataaaccaga accagcagcg
    15901 ccagcagctc ctgcaatgga tccatcaatg atgggcggta tgatgtagta attaagaaag
    15961 aaccctattt taacagggtt ctttcttctt tttttgtttt attttaagca aaaggggcaa
    16021 agaaggggca aaaatatcaa aggctattta gtttatcaat tacttgttgt ttggctcttt
    16081 ttgttacatg gttataaata gacagagttg tgtttgcatc agaatgccct acacgctcca
    16141 taatggcttt aagaggtacg cctaattcag ataatagtga aacgtggcta tgtctgaata
    16201 tatgtgagga tagacttttt tctaattcca actcttcctc tactttatgg agtatagcgt
    16261 taaatgagtg cagcgcaagc ggagtgccac ttgtagatat aaatatatat tgatcgggat
    16321 ctgtgggtct acctgcaaga atattgtcag ctatcacact ttcaatcaat tcttttgcac
    16381 gattgggtaa ttgcacttca cgttgcgaat aagtattttt tggagttgtt tttatagcat
    16441 tatccatttt cacagatgtg taatctaagg tcccattaat ggaaattttc ccatcctcat
    16501 agtccttcat ttgcaaagct agcaattccc catatctcaa accagttaaa tatagaaact
    16561 cagctattat gccgtgtagt tttctgcgag gattggagta tagctgtttc agtatttgat
    16621 caatttcttc tttctccaga tattttttat tcatagaaag ccttcttttt tcttcttcca
    16681 cttttttagg atggatttta actgctagcg cagggtttct ttgaatgtat tttctatcga
    16741 ttgcatagtt tagcataaca gatagagttg tttttgtttg ttttgtgtag ttcagtgaga
    16801 ggtcaccaaa cgtatacata tcttcaatta tcttattaat gagtgtctca tcaatgtttc
    16861 taacgatcgt atcatcgctt atgtgcttag aaacatgttt catcatcatt ggaaccttca
    16921 aatagctagt acgtttaaca tgctgcttat aatattcata ccattcttta tacagctcac
    16981 caaaagtgat atctgattta ttgtaatctt cgagtgcttc tttaattttt ttatctagaa
    17041 ttttctgagc ttttttccac gcttgtggtg aattacttgt aagtgttgta gatttttttc
    17101 gtgttttttc tgtataagga tctacatatc tttcaataaa cttgaattgg ccatctttgg
    17161 tctgttcaac ccacactttt aacatctcct atcatttgct ataataggca taacaaatag
    17221 acctatatag gtttgttttc taaaagcacg ctcttactct ggacggtggg gcgtgttttt
    17281 tatttattat acagttgctt taaaaatagc tgctttttca aatgatacta aaggagagaa
    17341 gtggatttct atttctccct tttggtttaa cgtgaaatga gcagttacat ccatttgttt
    17401 tcctggagca acagatccca ttgtattttc gttggcatat gtctctgatt ttttgtcatc
    17461 tggtccatat acttccacat ctgtacctac agggatatct gaatcgccat cattttttac
    17521 ggtataagta atttttacta cttgtgcagg ttggttttct tcaaattgat ttctttcatc
    17581 agttagttct acactattta gtgtatattc agcatcacca acttttacag tgtcaccaat
    17641 cttatagaag gtatcgcttt tttcttcttt agaagatgaa gcagaggttg aagatttagt
    17701 tactttttcg ccaccattat cacttgcttt attactatct gatccaccat ttaaagcaga
    17761 accaataatt ataattaaaa ttactgctaa tacccaaaac catacttttt tgtaaaaagg
    17821 tttactaact ttatacattt ttccgtcttg acccataact ttttttgcca tttaaatatt
    17881 cctcacttct tgttataata tatttgcgat ctcagaaatg aggtatgagt ccgtgtgcca
    17941 gcacgggctt ttttaatgtt ttgctgaaat cggttttttt aataattctt gatgtattca
    18001 tacatattgc cttgcgtaag aatatttttt cttaaaatag cattggcaga cagcataaca
    18061 agtgtatctg agcttattct gaatagaata atactccata aatttttcga gattaaactg
    18121 agattcatca gtcagttcgt tctcaatata gatatttaat aaaattagaa tagctatttt
    18181 atctgcttct gtttcaaatt ttgagtgaaa agttgtagaa gtatcgtaca aaactgaata
    18241 ttcaaaatga gaagcggtga aatgcgcaag ttcatgagat aaatgaaagg cttctgcagt
    18301 ttcgccatat agattttcat tcaaaaaaat gattctaggc ttcggataat agaagccagg
    18361 ctctttcatt tccatgtaaa ctactttcaa attgtattcg ctcaacattt ctttcaattt
    18421 caaatacata caacccatca ctccaactat tcattttcct ctaaagcttt agcaattgca
    18481 atcgctttac gcattgtctc cttagatatt tcttttccat caaaagaaaa aacagtatcg
    18541 ttttctgata aatccacatg tttaggggtt tctctttctt ctctacctag aaggtagtct
    18601 acagagactc caaagtaatc agctaaccga gctaactcaa tagaattagg agtttgtctt
    18661 ttccaacttc ctaaatatcc attcgaatac ccgaaattca tttcgagctg tctaatggat
    18721 aatccttttt cttttgctaa ctcctttatt atttcgtaag tattcattga taaatcaacc
    18781 tttctgaatg cttacaaaaa aagtttagat aaataatgta aaaatagttg acttatatac
    18841 attattaatc tatactatgt tttgtaaaca agtttaacaa ctaaaaagac aacaaaaaat
    18901 actattgatt aataaatgcc aaccgccaag aaagcttttt aaatcaaagt ttatatgtct
    18961 tatttaacta tgcatttagt atagattatt aatctaataa agtcaacgca gttttttaaa
    19021 aagttgttaa atttgtttac gaatataaaa gaaaggagag aagaatatgg aaaaaacagt
    19081 ctcggcaaaa atcgaagatt tgaaattaga catactgaaa caagcaaaag tggcgatgga
    19141 acacgcggta gataaagaag actctgccat ggttgcagcc atagcagaga ttttagctca
    19201 cgtttagtca tcgtttttac ggtcttctgg aagctgacca tagataacag cataatgaat
    19261 atctaaataa gcttgaacaa tttcttttgg cgaaattgat tcgcctttag tagatacttg
    19321 tgattcgtga tttagccatg caacaactaa atccgcagcg atttcaaacg gcgttacact
    19381 ttgtgtcagt tgccataaag aatttcacaa tatgtatggt cgtggtaata ataccaaaga
    19441 gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa tcatttatta aaggaggcta
    19501 aacaatgaac aaggaaatga gccaaacaat caaggtacaa aaaatgatag atgatctaac
    19561 acatggaatt gatagccaag ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa
    19621 ggatgcaaaa atgcttctca agactatcaa ctttgaaatg aatccaacaa aaagaaagct
    19681 cgccgatgtg ttggaagaaa agttggcttc cgctatcaac gagcaagaat tactatttga
    19741 aaccgatact tttaacagct gatgttttat agatgacagt ttcatccgaa tctatatcat
    19801 cgccgagaga aaagaatgtt gcttttgaca aaaagccgat aagacctaca agcggtcttg
    19861 atgttgcaag agtagatacc ccaatatcac cttgcaaggt attcacgtaa atttgagaag
    19921 aatagaacat tccatctttt tcttcgatat ttgacgcatc atcgtatcca acgaccaaag
    19981 tatcggcaaa tattttgatt ttttctccat caagcatagt aattacaaat tctggtttca
    20041 taaattttca cctccttaac aattatttca gcctgtcaca ctgataagga aattatacca
    20101 aagaaaggaa tgagaaatat gaatacacca caaattttta atttcgagca aaacgaagtt
    20161 cgaactattt tagtaaatga tgaaccatat tttgtaggaa aagatgtagc agacgttcta
    20221 ggatattcga atccgcaaaa agctattcga gatcatgtgg acttggagga taagacgcag
    20281 aacgattcgt tcaccgtcaa tggaacagca gttgttttga ttaacgagtc aggcctttac
    20341 agtttaatct taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacgagt
    20401 gaagtccttc caacaattag aaaaacaggt agctattcaa acgtacctca aagttttgca
    20461 caagcattgc gtttagcagc ggatttagaa gaaaagaacc aattactcga acaacaaatt
    20521 gccgagtacg aaccaaagat tagctactta gatacgattc tttcatcgac agatacggta
    20581 gctacttctc aaattgcagc tgattacgga atgtcggcaa ttgctctaaa caaattgcta
    20641 aacgagttag gtgttcagca taaagttagc ggacaatgga tactttaccg aaaacatatg
    20701 aaccaaggat acacaaaatc gcacacaagt gagataccga aagccgatgg cggcactaaa
    20761 gttgtaatga ataccaaatg gacacagaaa gggcgagtgt ttatttacaa cttattaatc
    20821 gcagagggct attaccctca aatggattta gaggaaattg gttagaaagg agttttagta
    20881 tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa
    20941 gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact
    21001 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga
    21061 ttatcactat gtacgaattg tagagggagg taaagtcgtt ggaagataaa atcattctta
    21121 caaaatcaga gcttcaagaa atgttaaaca tcgagtatgg aacgataatt tttaggaggg
    21181 ataataagtg aaaaaaccaa cactttcaga gctgatagaa gctgctgaga aggcagtaaa
    21241 accagacgac tggtaccgac aaagtttaat cttggagaag ttccacggca tgtcaaaaac
    21301 tactttagtt gaatactgca aggaaatgga aacaattcct gaatttgcag aaggaatact
    21361 tcgtccaggt cactcaacca catttattca ttaccatact tttatttggt ttttaaagtg
    21421 gaaagacgca aacaaatatc gtgtaaaaag tttatcgcct tcagatgttt tgaaggaggc
    21481 aagttaatgg gcaaattcaa cagagcacta gtattcagcg caccgctaat catctacgct
    21541 ttaggcctct ggggaagcag acaagcgttg atagggacaa tcgtttacat ggtttggatt
    21601 tttatggggc ttgatgaagc tgagcacaga gcgaaaaaag ccagtcggga gggactgact
    21661 aaaatatgat ttttttattt ttgttccttt gggctgctat tgcgtacttc atccagctcc
    21721 ttgttgtcaa agttgctctt atttctttta ggaagaacaa aagaaaatag cgctataaat
    21781 gaaagataag tagccgctac attaaactgt tcttttgaaa aagttacagt catcggaatg
    21841 ataattgata ttgaggaagt aatcttaatt aaaaatatag caatattttc aacatcgcgt
    21901 tggtttttat ttaacattgt ccaacatgtt ttagcaacaa taaaaataat tacacatgat
    21961 attagcacac agactgaaaa aagttttaga aatgcgatgt agtcagtgtt attgaactcc
    22021 ttcaaaatac tatttttatt caatgtaata aaaggaattt ttttaattga agacaggaaa
    22081 gataaggtaa taaacagaat tatatatatg gttaaagtcg cagtaagaat gtaattgagc
    22141 tttttcctca tagaatcacc tcactttcta acgaaaatta taccaaaaag gagagaagaa
    22201 ataatgcaag aattagtaat tttgaaaaat aaagaagctg taactacgag cttacaagtg
    22261 gcagaaagtt tcaaaaagaa acataagcat gtactagaag caattgaatc aataaaaaga
    22321 tcggtcgaaa attcggccaa tgttgaagat gggtccaatt ttggacagat gtttgtggaa
    22381 gggaacgagc cagactcata cggaagaagt cggagagttt atttcatgaa tagagacgga
    22441 ttttccttgc tagctatggg attcactgga agtaaagcaa taaatttcaa actaaaattt
    22501 attgaagctt tcaacgaaat ggaagatgtt attcggaaga atactgttcc tcaaacaatt
    22561 gaagacatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg
    22621 cttaaagata ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat
    22681 atgaaagtta tggaagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa
    22741 aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc
    22801 gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttaccagaa
    22861 actgcgatcc gcatggaaat cgatcaactg aacagacaac aaagactttt cggtgatgaa
    22921 aatgaataga gctgaagcgc taagaatagg gacggtaatt gctaatcgct ggtggagaca
    22981 caataaacca aacatcctaa gccaacaaca tatcgaaaag caaaaagcat ggcaacaaat
    23041 aaaaaagtga ctcagccggc aagcatagag tcacaaaaca tatctaagga gaattttagc
    23101 atatgaataa agaactttcc actttagatc aatatttgac tgattctgaa tggggcaagt
    23161 cgaatatcaa ggaaacaaat aatcgaaaaa tcagacgaaa tcttttgacg aacgaagaac
    23221 tatcatgtga tcaagatgac ttgggaaatt ttgtgagtat ttgggatcat gtttacctta
    23281 tccatctatc aaaacattca aataaacctg aatacatcta tgtcatcgaa gatggcttga
    23341 ttgatgcgct agaagagtac aacagagata acttgattga tatctcttat tacggatcag
    23401 gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg
    23461 aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt
    23521 gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg
    23581 gtaactatta atgagtataa acactacagg gttgtttctg gaactcatca agactttctt
    23641 gttgaggaat ataaaccttt tcttatggat gaaacaggga cttatgtatc tgtctcagta
    23701 acggttaaag gacatacgga aacaga
//
LOCUS       DAYYJC010000035        23434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000035 DAYYJC010000000
VERSION     DAYYJC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.035"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1773
                     /locus_tag="ACHA9N_001343"
     CDS             112..1773
                     /locus_tag="ACHA9N_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9084812.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1843..2190)
                     /locus_tag="ACHA9N_001344"
     CDS             complement(1843..2190)
                     /locus_tag="ACHA9N_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9084813.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2274..3215
                     /gene="opp3b"
                     /locus_tag="ACHA9N_001345"
     CDS             2274..3215
                     /gene="opp3b"
                     /locus_tag="ACHA9N_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9084814.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3216..4259
                     /locus_tag="ACHA9N_001346"
     CDS             3216..4259
                     /locus_tag="ACHA9N_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084815.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4265..5335
                     /locus_tag="ACHA9N_001347"
     CDS             4265..5335
                     /locus_tag="ACHA9N_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084816.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5325..6278
                     /locus_tag="ACHA9N_001348"
     CDS             5325..6278
                     /locus_tag="ACHA9N_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084817.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6290..7300
                     /locus_tag="ACHA9N_001349"
     CDS             6290..7300
                     /locus_tag="ACHA9N_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9084818.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7585..8406)
                     /locus_tag="ACHA9N_001350"
     CDS             complement(7585..8406)
                     /locus_tag="ACHA9N_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9084819.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8503..8781)
                     /locus_tag="ACHA9N_001351"
     CDS             complement(8503..8781)
                     /locus_tag="ACHA9N_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084820.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9049..11058
                     /gene="metG"
                     /locus_tag="ACHA9N_001352"
     CDS             9049..11058
                     /gene="metG"
                     /locus_tag="ACHA9N_001352"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9084821.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11537..12124)
                     /locus_tag="ACHA9N_001353"
     CDS             complement(11537..12124)
                     /locus_tag="ACHA9N_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084822.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12299..13621
                     /locus_tag="ACHA9N_001354"
     CDS             12299..13621
                     /locus_tag="ACHA9N_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9084823.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13776..14552
                     /locus_tag="ACHA9N_001355"
     CDS             13776..14552
                     /locus_tag="ACHA9N_001355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9084824.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14545..15114
                     /gene="rnmV"
                     /locus_tag="ACHA9N_001356"
     CDS             14545..15114
                     /gene="rnmV"
                     /locus_tag="ACHA9N_001356"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9084825.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15107..15991
                     /gene="rsmA"
                     /locus_tag="ACHA9N_001357"
     CDS             15107..15991
                     /gene="rsmA"
                     /locus_tag="ACHA9N_001357"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9084826.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16442..17071
                     /locus_tag="ACHA9N_001358"
     CDS             16442..17071
                     /locus_tag="ACHA9N_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084827.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17195..18304)
                     /locus_tag="ACHA9N_001359"
     CDS             complement(17195..18304)
                     /locus_tag="ACHA9N_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084828.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18513..18935
                     /gene="mgsA"
                     /locus_tag="ACHA9N_001360"
     CDS             18513..18935
                     /gene="mgsA"
                     /locus_tag="ACHA9N_001360"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9084829.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19122..19220
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19258..19794
                     /locus_tag="ACHA9N_001361"
     CDS             19258..19794
                     /locus_tag="ACHA9N_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9084830.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19834..21597)
                     /locus_tag="ACHA9N_001362"
     CDS             complement(19834..21597)
                     /locus_tag="ACHA9N_001362"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084831.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21600..23315)
                     /locus_tag="ACHA9N_001363"
     CDS             complement(21600..23315)
                     /locus_tag="ACHA9N_001363"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084832.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7548 a         4293 c         4769 g         6824 t
ORIGIN      
        1 tatgaaaaag acaaaaaaaa gtgttgattt tttaataatt attcattaaa ctcttaaaca
       61 acttcagaaa atattaatta ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag
      121 aaatttacat ttggtttggt tgcggtatgc ggccttgtac ttgctggatg ttatggcggc
      181 agttcagcca caagcagtag ttcagcaaaa agcggttcag cagatggagg aggcgtattt
      241 aacttagtcg tgcctcaaga aatgcctaca gctgacttat cagttgcaac agatacaatc
      301 agttttacag cattgaataa cgtttatgaa ggaatttacc gtcttgatga agacagcaaa
      361 ccacagccgg caggagctag cgagttggca gaagtcagtg aagacggatt aacttacaaa
      421 atcaaattac gagaagacgc aaaatggtca aatggtgatc ctgttacagc agcggattac
      481 gtttatggct ggcaacgaac agtagaccca gctacagctt ctgaatatgc ttacctgtat
      541 gcgccagttg aaaatgcaga agacattaca gctggaaaga aagataaatc agaactaggg
      601 atcaaagcag ttggtgacta tgaattagaa atcaaattga cgaaacaaac accatatttc
      661 caatatctgc ttgctttccc atcatttttc ccacaaaaac aatccgtagt ggaagaaaat
      721 ggggatgcct atgcatctgc aagtgataaa gctgtttata atggaccatt cacattagaa
      781 gattttgatg gaccaggaac agatacagaa tggacttata agaaaaatga tcattactgg
      841 gacaaagata cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga atcttctact
      901 gcattgaact tattcaaaga tggccaagca gatgacgtga tcttgtctgg tgagttagca
      961 cagcaaaacg caaacgatcc agcttatact tctgttaaag aagcgcggac aagctatatc
     1021 gaattcaatc aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag aaaagctatt
     1081 tcttattcta tcgatcgtga agctttagta aaacaagtac taggtgacgg atcagttgtc
     1141 tctacaggat tgatcccatc agatatggta aaaagtccaa caacgaacga agactttgca
     1201 aaagaagctg gaaaattagt ttcttatgac aaagataaag caaaagaata ttgggaaaaa
     1261 gcgaaaaaag aattgggcgt tgattcttta gaatttgatt tgatggcttc tgatgatgat
     1321 tcatcgaaaa aagtaatcga atatgttcaa aactcaatcc aagaaaactt ggatggcgta
     1381 acagtcaaac caacacctgt tccattctct gtacgtcttg accgctcaac ttcgggtgat
     1441 tttgacgcag tactaggcgg atgggctgct gactatgcag acccaagcag ctttactgat
     1501 ttgtttgtca caggtaattc atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa
     1561 gcagttaaag cagcttcatc aacagacgct ggcgatgaac aagcccgttg ggatgatttc
     1621 caaaaagcaa tcaagatcat ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca
     1681 gaaggtcacc tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca
     1741 tgggattaca aatggacata cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa
     1801 acgaggctgc ggcaaatgtc gcaacctcgt tctcatattc acttatccta acaaagaaaa
     1861 aagcatgaaa atagcagaaa gagcaattag tattccggca atgatcagcc aaaagctgta
     1921 cataccgtag ctggcagacg agagcgagga aaattctggt tttttctttc ggttccttgt
     1981 tcgtgcaaga tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa agacccataa
     2041 aaaaccgccg ataatcagaa acggcaatga ccataagaat agatcattag ataattgaat
     2101 gagtgtaaag gaatggttga tgagattttt tattaaaata accagaatca gtagaaaagc
     2161 tactgcgtag gggatagatt tttttttcat ttgattgctc ctttattttt ggttcattat
     2221 ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata
     2281 gttatatcaa atatgtatta aaacgtgtct ttttcatgat catcacgctt tggttaattg
     2341 caacaatcac ctttttcttg atgcaattgc tgccgggcac gccatatacg aatcaggaac
     2401 gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt cggtttggat aaaccagtta
     2461 tcgtacaata tgggatttat ctttctaatt tgcttcaagg agattttgga atttccttcc
     2521 aatttaaaaa ccagccagtc gcacatttgt tagctggaag aatcggacca tccttgcagt
     2581 tagggttaca ggcaattatc tttggaacag tcctagggac gattttagga acgatctctg
     2641 ccatgaagca aaatacatgg gcagatacat cttctacact cgtagcgata ttaggtcgtt
     2701 ccattccaaa ctttgtattt gctgttttat tacaatatat ctttgcaatc aaattacaag
     2761 ttttgccaat cgcaaaatgg gatggctttg tgtacacaat cttaccaaca attgcgttgg
     2821 ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac ggaaatggtg gaagtactgc
     2881 atagtgatta tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa atcgccttta
     2941 aacatggtct tcgaaatagt ttgattccat tgatgacttt attaggaccg ttagctgtag
     3001 cattgatgac cggatcactg gtggtagaaa atatctttgc tattcctggt atcggggaac
     3061 aattcgttaa gtcaatcaca acaaatgatt atcctacgat catggctgta acgatccttt
     3121 actcatttat gctgattttt gttattttga tcgtggatct attgtacggt ctagttgatc
     3181 caagaattcg tgtttcagaa gggagccgag gataaatgga aatgatacca aagcgttatg
     3241 aaacagttgc agatatccct gcggatgaat ttcttccatt acaaaaaaat accgaagaag
     3301 aacgtgaaca gattactgca ccttctttga attttatcca agattcatgg cgccgtttaa
     3361 agaaaaacaa agctgccgtt atttcaatgg ttttactatt ggtcatcatt ttgatctctg
     3421 tgatcacaat ctttgtttct cctcatgatc caactgccca aaacgtggct tatatcaatt
     3481 tgcctcctcg tattccggga atcaatatcg atggattgaa tggaaaagca atggttggtg
     3541 gtgaacttgt tgataaatat gctcaagcaa acgtcccttc aaacgtaaat ttctttttag
     3601 gaacagacgg tttgggacgt gatgtattaa gccgcttatt tatgggaatg agaatctcgt
     3661 tgctgatcgc atttattgct gcattactag atgtaacgat tggtgtagct tacgggttgg
     3721 tttctggttt gttaggcgga cgtgtcgata acgtgatgca acgttttttg gaaatcttat
     3781 caggaattcc aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa ccaggaattt
     3841 tttcaatcgt ggctgctatg gccatcacaa actggatacc aatggcacgt atcgttcgtg
     3901 cacaaacaat gaaattaaaa gatcaagaat atgttttagc tggattgaca ttaggtgaat
     3961 caagaatgaa aattgcattc aaacatattt tgccgaatat ctctagtgtc attattattc
     4021 aaatgatgtt tagtattcca tctgctattt tcttcgaagc ctttttaagc tttatcggtt
     4081 taggtcttac gcctccgtca gcttcattag gaacgatgtt gagcgaaggg tacaagacat
     4141 tcctgtatct gccttatcta ttatggattc cggcagtaac tttatcggtt atcatgattg
     4201 gctttaactt acttgctgat ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa
     4261 gacgatgacg aaaattttag aagtgaaaga cttagaaatc tcttttgata cgtatgctgg
     4321 aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa cattggcgat
     4381 cgttggggaa tctggaagtg gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc
     4441 aagcaacgcc aatatagatg ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa
     4501 atcagaaaga gagatgcaaa aaatccgtgg taaagaaata gcgatgattt tccaagatcc
     4561 aatgacctct ttagatccga cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa
     4621 gcataataaa gtttcaaaaa aagagggaca aaaagctgca ttagaattgc ttaaattagt
     4681 tggaataccc gaagctgaga aacgtatcaa cagttatccg catcagttct caggaggtca
     4741 gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat ccggaaatcc tgattgctga
     4801 tgaacctaca acagcgcttg atgtgactat tcaagcacag attttggagc tgctgaaaga
     4861 aattcagaca aaaatcgata catcaattat ctttatcact catgaccttg gtgtagtagc
     4921 gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag ttggaacatc
     4981 ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc
     5041 gacattagat agtgcgaacg atcgattgta tgctattcct ggttctccac cggatttatt
     5101 gaacccgcca aaaggagacg cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc
     5161 agaacaagaa ccaccatttt ttgaactatc aaaaacacac aaggcagcaa cttggctact
     5221 tgctccgcag gcacccaaag tgacaccgcc agaagaaatc caacgtcgct gggctatctt
     5281 taaagagaaa caaaaacata cttacggtca aggaggaatt gctgatgcca aataaactac
     5341 ttgaagttaa aggattgaaa cagtatttca atgtaggaaa aaagaatgaa gtacatgctg
     5401 taaatgacat cagctttcac atttatgaag gagaaacatt cggtttagtt ggagaatctg
     5461 gaagcggaaa atccactact ggccgtacga tcattcgctt gaatgaacct actgatggtg
     5521 agatattatt cgacggtcaa gatataacga agatcaaaga taaaaaaggt ttgacgaaat
     5581 tccggcatga tgttcagatg atctttcaag atccatacgc ttcgttgaat cctcgtatga
     5641 aagtcagaga tatcattgcc gaaggaattg atgttaatgg actagctaaa tcatctgaag
     5701 agcgtgcaaa aaaagtcgat gatctattga gaactgttgg attgaatcca agccacggta
     5761 ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg tattggtatt gcacgagcat
     5821 tggcagtaaa accgcgtttt atcatttgtg atgaaccaat ttctgcacta gatgtgtcga
     5881 tccaagccca agtggtcaat ttgttgcaag atctccaaaa agaacatcaa ttgacctatc
     5941 tatttattgc acatgatttg tcgatggtta aacatattag tgaccgaatc ggtgtaatgc
     6001 ataacggctt gttattagag atgggaacaa gtgatgaaat ctataatcac ggtgttcatc
     6061 cttatacaga aagtttgtta tctgctattc ctctaccaga tccagaccat gagagaaaac
     6121 gcagaagaat caaatatcaa cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa
     6181 tcgcaccaga acattttgtc tatgcaactg agcaggaagt cccatattac gcaaaaaaac
     6241 taaaacgaca aaaggaatct cttctggttg ctaactagga gggacataga tgctaaaagc
     6301 ttttaaattc cgtatgtatc cgacagaaga acaaaaacag caattgattc gtacttttgg
     6361 atgtgctcga tttacctata accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac
     6421 aggagtagcc aatttttcgc tcacacccgc tacattaaaa aaagaatacc cattcttaaa
     6481 agaagtagac agtcttgctt tagcaaatgc acagcttaat ttggatcggg cttttcgcaa
     6541 ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc
     6601 ttatacgacc aacaaccaaa cccgtacgat ctatctggaa aatggctact taaagttacc
     6661 aaaacaaaaa gaactaatca agataaatca gcatcggcca gttgaaggat cgattcgctc
     6721 cgctacgatt tctgctcgat ataatgaaga attttatgtc gcactacttt gtgatgtttc
     6781 gccagtaaaa aaagaatctt tagctaaatg gatcgggatc gcctatcatc cgaagacatt
     6841 gatccaaaca tctcgacctc tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa
     6901 gctgcaacat gcacagcgga aattgaatgt gaaagtccgc tccgctcacc atagaaagat
     6961 caggttagat caggcaagca attatcagaa acaaaagaga aaagtaatgg atctttatct
     7021 gaaacaaaag aaccagcgag aagattatct tgaacaatta tctgggaaat tggtcaaaca
     7081 atatgactac ttatttgtag aatcttttcc aaaagaagag gcacatgctg atttttctat
     7141 tcacgactgg cataaattaa taacaaaatt gcgttataag tcccaatggt ataataaaaa
     7201 atttcttttg atcaataccg acggagcaga agaaagcaat tccgtaagaa agagccaagt
     7261 agtagaaaaa atgggcagac attcggttat caagggataa aaaatgaatg aaactgatag
     7321 gagcgttagg gttagccttg ttaataaaaa gtcttaatga cttttgtttc taggaatttt
     7381 tcttttgaag aaaagctcac taattatgac aaaccaataa aacaatagca gaaaaagttc
     7441 gtacgagctt tttctgctat tgttaatttt ttattcagaa agtttacgaa tacatcaaaa
     7501 ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct tgaatgatta
     7561 ggtctgaatc ttttgctcac aaccttattc atggtgcccc ggcttgagct gatagatttt
     7621 tgaaggacgg ccgattccta caggacgttc accgatctca tcgaaatatt ggagcatagc
     7681 tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc cctaagaatt tagcaaatac
     7741 ttttcgagct tccgtaatcg tgaaatcttt acctaaaact tgtagaactt gcggctcgtg
     7801 ttccatttta ttgactacac ggttgaatgc tttcaagatg atttgactat ggtcaaaagc
     7861 caaagtatct ttgccagaag actctccagt gactaaatca agcgagatag ccacttctcc
     7921 gctagataaa tggatcttgt tactatgacg ttctagtgtg aaccagcgta cttccttggc
     7981 atcatctccg gcgattagcg gttcttctcc aataaaggct aagtagctga ctgtgacgac
     8041 ccagcctcgc ggatctcgat taggcgtact gaatgtatgc agttgctcaa tattttcttt
     8101 tgaaataacc acaccagttt cttcctttgt ttctcttaag acgctttctc cagttgactc
     8161 atccttttgg acaaagccac caggcaatgc ccatgaattt cgataaggat gcccttttct
     8221 ttggatcaac aaaactttca gctgatcttc ttctttgtta tagcacatca gtacgatatc
     8281 gacagtcaaa gagggcttgt catattctgg acgttcctgt ttttgatacc agttaagaaa
     8341 ttcttcttca cttgctttat gttcataata taatttttct tcttctttag aagcaaaacg
     8401 gttcatctga ccacctccat tgacagtaaa agtacttttt accttattgt agcagactct
     8461 gaaaaaaaac caaggattta cttgaaaaat ctaggttatc atttaaatat gaaaaataaa
     8521 tggaagtatg agattgaata aaaagaaaag cagataaaac ggcaatactt tataccgctc
     8581 ttcacgaatg gcaaagaagc cacaaataac gatatatacg tcaatgataa aacgcaaaat
     8641 ccaagctgct gtcataaaac tgttcaaatg cccatggagg ttaaaataac catacatacg
     8701 aatataaaaa tagctgaata ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa
     8761 aaaaagatgt ttcttctgca tgattgttcc agtcccttac taagatagat agtctttatt
     8821 ataccacgtg tgaggggaga aataaaaagc cgttttttag tagatatgaa acaaaacaaa
     8881 aacccgcatt tattcaacct attcatcctt ttaaaggata gattgaacgt agatagaagt
     8941 cgtttatcca gtctttttca ttttgaaaaa aagctaacag catgcttttt ttatggtata
     9001 ctagcaagga aacgaattcg catcatgcga taaaggagga agagccccgt ggcagaaaag
     9061 gaaacttttt atatcactac gccaatctat tatccaagcg gcaaacttca tatcggcaac
     9121 tcgtatacta cgattgcatg tgacgcaatt gctcgttata aacggttgat gggctttgat
     9181 gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa
     9241 ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg
     9301 aaaacgcttg atatcagtta tgataaattc attcgcacga cagatgatta ccataaagca
     9361 gctgttcaaa aaatatttga tcgtttatta gaacagggag atatttactt aggtgaatat
     9421 gaaggctggt actctgtttc tgacgaagaa ttctttacag aaacacaact tgcagaagtg
     9481 tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa
     9541 ttggtaaaag aagaatcata cttcttccgc atgagcaaat acgcagaccg tctattggaa
     9601 tactatgaag aacatcccga attcatccag ccagaatctc gtaaaaacga aatgatcaac
     9661 aactttatca aaccaggatt agaggatctt gcagtttcac gaacaacttt tacttgggga
     9721 gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc attatccaat
     9781 tatattactg cactaggata tggctccgaa gatgaaagtc tattcgaaaa atactggcct
     9841 gcagatgttc agatggtcgg taaagaaatc gttcgctttc atacgatcta ttggccaatc
     9901 atgctgatgg cccttgattt gcctctacct aaaaaagtat ttgggcatgg ctggctattg
     9961 atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg
    10021 gaacgttatg gtttagatgc actccgttat tatttgcttc gtgctgttcc gtttgggtca
    10081 gatggtgtct ttacgccaga agattttgtt tcccgtgtca attatgatct ggcaaatgat
    10141 ctagggaact tattgaatcg aacgattgct atgatcaaca aatattgtga tggactcgtt
    10201 cctgactatg cctcacttgt cactccattt gatagtgaac tgtctacgac ggctgctaac
    10261 gttgtcggac gttaccatga tgcaatggaa aaaatggaat tcaatacagc attggctgaa
    10321 atctgggtgc tgatttctcg tgcaaacaaa tacattgatg aaacagaacc atgggtatta
    10381 gcaaaagacg aagaaaagaa agctgaattg aatagtgtca tgatccattt agctgaatct
    10441 ttgcgtatcg cagcgatatt acttcagcca atcatgacag aaacgccaga gaagatcttc
    10501 aatcaactag gattggactc tgaaacaatg aatcttgaag gattacactt tggcgaattt
    10561 ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct tcccacgttt agatatggaa
    10621 gaagaagttg cttttattca agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca
    10681 gtcaaatggg atccagaaga aacagaactt gtttcaacga aagaaaaaca aatcaaattt
    10741 gatgtatttg aaaaagtaga actaaaagta gcagaagtca tcaactgtca aaaagtagaa
    10801 ggggcagata agttgctgca attccgacta gatgctggag acagccaaga ccgtcaaatc
    10861 ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc
    10921 attgtagcaa atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt
    10981 tctgcagaag caccagatgg ctctttacaa gtaatcgaag caccaaaatc catgccaaat
    11041 ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc ataccccctt
    11101 tttccgaaat ttgagaagca attttcgtaa attccttctt attacttgaa gccaatcata
    11161 taatacagaa acagagaact atccaaaata aatcctagaa aaagaagtac gagcttcgct
    11221 gatttttgtt aatttagaat aatactaata ggaatcgtaa aaacagaaaa aaagcataat
    11281 gataaagggt cgggcagatt ttaattgatt catctggtac tcttttttat tgtccttaca
    11341 ttcataaagt ttgaatgcaa tgactttatt acatctatat ttatatctaa cagtagcgtt
    11401 gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt
    11461 gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta
    11521 ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc
    11581 ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac
    11641 gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta
    11701 aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca
    11761 tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg
    11821 taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa
    11881 aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca
    11941 atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag
    12001 tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac
    12061 gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat
    12121 ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa
    12181 atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta
    12241 aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat
    12301 gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac
    12361 cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac
    12421 aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac
    12481 agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc
    12541 tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc
    12601 tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt
    12661 atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa
    12721 tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat
    12781 accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt
    12841 atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt
    12901 agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa
    12961 acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt
    13021 ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa
    13081 tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt
    13141 cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat
    13201 ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa
    13261 gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg
    13321 tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg
    13381 gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt
    13441 gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc
    13501 tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg
    13561 tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta
    13621 aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt
    13681 tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt
    13741 atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt
    13801 taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg
    13861 gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat
    13921 taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca
    13981 gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag
    14041 cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata
    14101 aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata
    14161 ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg
    14221 gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag
    14281 ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg
    14341 ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac
    14401 cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga
    14461 ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc
    14521 accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt
    14581 gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca
    14641 atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt
    14701 ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg
    14761 gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa
    14821 aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag
    14881 gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta
    14941 gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag
    15001 ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt
    15061 acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg
    15121 tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa
    15181 aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac
    15241 tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac
    15301 ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat
    15361 ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat
    15421 tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa
    15481 agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc
    15541 agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc
    15601 tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga
    15661 agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc
    15721 agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc
    15781 atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt
    15841 gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac
    15901 agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt
    15961 aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa
    16021 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg
    16081 tttgtttggg cttgttaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt
    16141 gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt
    16201 ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg
    16261 agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat
    16321 acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat
    16381 tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa
    16441 aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt
    16501 cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc
    16561 tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct
    16621 aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc
    16681 acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt
    16741 aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg
    16801 tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat
    16861 gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg
    16921 tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag
    16981 caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat
    17041 tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat
    17101 ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc
    17161 aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg
    17221 tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg
    17281 gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt
    17341 aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt
    17401 caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga
    17461 taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac
    17521 gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt
    17581 tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt
    17641 ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca
    17701 tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt
    17761 gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg
    17821 gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt
    17881 ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga
    17941 tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca
    18001 agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa
    18061 ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta
    18121 gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac
    18181 aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag
    18241 ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta
    18301 ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa
    18361 gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc
    18421 tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa
    18481 gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc
    18541 gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg
    18601 aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc
    18661 atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg
    18721 atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag
    18781 atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg
    18841 gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg
    18901 tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg
    18961 tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat
    19021 acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga
    19081 aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag
    19141 cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt
    19201 ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg
    19261 aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg
    19321 gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt
    19381 attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt
    19441 ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca
    19501 ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact
    19561 tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca
    19621 ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc
    19681 cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga
    19741 cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca
    19801 agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata
    19861 gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat
    19921 agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc
    19981 aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc
    20041 tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat
    20101 catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc
    20161 gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc
    20221 ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc
    20281 tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa
    20341 tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt
    20401 gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac
    20461 tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt
    20521 ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc
    20581 caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc
    20641 tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa
    20701 tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga
    20761 cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg
    20821 atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt
    20881 ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa
    20941 agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat
    21001 aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag
    21061 gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat
    21121 aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga
    21181 aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga
    21241 gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa
    21301 ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac
    21361 tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg
    21421 gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac
    21481 tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc
    21541 agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac
    21601 tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct
    21661 tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct
    21721 tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc
    21781 gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc
    21841 agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca
    21901 gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca
    21961 aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc
    22021 ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca
    22081 agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt
    22141 ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc
    22201 ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata
    22261 ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata
    22321 attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct
    22381 gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg
    22441 aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga
    22501 acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag
    22561 atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa
    22621 acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt
    22681 tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca
    22741 ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata
    22801 aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag
    22861 tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa
    22921 cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc
    22981 aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt
    23041 agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa
    23101 atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg
    23161 atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc
    23221 aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta
    23281 cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt
    23341 ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag
    23401 aacaagcctt ttcataaatg aaagttttat tatg
//
LOCUS       DAYYJC010000036        23258 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000036 DAYYJC010000000
VERSION     DAYYJC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.036"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..686
                     /locus_tag="ACHA9N_001364"
     CDS             <1..686
                     /locus_tag="ACHA9N_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9084833.1"
                     /translation="LYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLS
                     WVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSI
                     IYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGL
                     FYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIAR
                     RFDSDSALF"
     gene            698..1618
                     /locus_tag="ACHA9N_001365"
     CDS             698..1618
                     /locus_tag="ACHA9N_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084834.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1659..3116
                     /locus_tag="ACHA9N_001366"
     CDS             1659..3116
                     /locus_tag="ACHA9N_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9084835.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3461..4075
                     /locus_tag="ACHA9N_001367"
     CDS             3461..4075
                     /locus_tag="ACHA9N_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9084836.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4068..5792
                     /locus_tag="ACHA9N_001368"
     CDS             4068..5792
                     /locus_tag="ACHA9N_001368"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9084837.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5789..7240
                     /locus_tag="ACHA9N_001369"
     CDS             5789..7240
                     /locus_tag="ACHA9N_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9084838.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7713..9866)
                     /locus_tag="ACHA9N_001370"
     CDS             complement(7713..9866)
                     /locus_tag="ACHA9N_001370"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9084839.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9868..10914)
                     /locus_tag="ACHA9N_001371"
     CDS             complement(9868..10914)
                     /locus_tag="ACHA9N_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9084840.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11064..12353
                     /locus_tag="ACHA9N_001372"
     CDS             11064..12353
                     /locus_tag="ACHA9N_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9084841.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12366..15068
                     /locus_tag="ACHA9N_001373"
     CDS             12366..15068
                     /locus_tag="ACHA9N_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9084842.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15053..15910
                     /locus_tag="ACHA9N_001374"
     CDS             15053..15910
                     /locus_tag="ACHA9N_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9084843.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15981..16838)
                     /locus_tag="ACHA9N_001375"
     CDS             complement(15981..16838)
                     /locus_tag="ACHA9N_001375"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9084844.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17049..19220
                     /locus_tag="ACHA9N_001376"
     CDS             17049..19220
                     /locus_tag="ACHA9N_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9084845.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19288..19629
                     /locus_tag="ACHA9N_001377"
     CDS             19288..19629
                     /locus_tag="ACHA9N_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9084846.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19905..21659)
                     /locus_tag="ACHA9N_001378"
     CDS             complement(19905..21659)
                     /locus_tag="ACHA9N_001378"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084847.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21670..22506)
                     /locus_tag="ACHA9N_001379"
     CDS             complement(21670..22506)
                     /locus_tag="ACHA9N_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9084848.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22508..23191)
                     /locus_tag="ACHA9N_001380"
     CDS             complement(22508..23191)
                     /locus_tag="ACHA9N_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9084849.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7644 a         4157 c         4784 g         6673 t
ORIGIN      
        1 ttttatataa tgtagcattt atccttttga atctgctgat ctcagtcgta tttgcaatca
       61 ttatgagcga gttgagaaat aaacgactcg ttaaagttta tcaaacgatg tccttactac
      121 cttattttct atcttgggtc attatcagtt actttgttta cgctttttta agtcctgaca
      181 aaggaatatt caatcagtgg atcactggac atggaggaga agctgtcaat tggtataatg
      241 aaccgaaata ttggccgttt atcttggtgt tcattggtac atggaaaggg attggttata
      301 acagcattat ttactttgca tctgttatgg gaatcgatcc gacttattat gaagctgcta
      361 tggttgatgg agctagcaaa tggcagcaga tcaaacatgt gacgatacca caacttattc
      421 cactaatgac gattttaacg attttagccg taggaaacat tttccgagct gattttggcc
      481 tgttttataa cattcctagg aattccggtg cattatatga tgtgactcaa gttttggata
      541 cgtatatcta caatggcctg acatccacag gagattttgg tatgacggct gctgcaggac
      601 tttaccaatc agttgtcggc tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt
      661 ttgatagcga ttctgcatta ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag
      721 agtccaagtc cgctctttca ataagacggc taattttttc tctaatataa tgattgcact
      781 ctttgctatt tcctgtattt tgccatttat atttgttatc gccatctctt ttaccgatga
      841 gtccatattg tcgactcacg ggtacagttt ttggccagaa aagttcagta tatttggtta
      901 tacgtacttg ttcgaccaga tgcaggataa aattttccaa gcgcttttcg tcactgtact
      961 tgttactgtt ttaggtacac tgatcaatag cacggctact tctttatatg cttatgcgat
     1021 ttcacggtca aactttccat tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt
     1081 gttctcacca gggatggtgg ccaattatct tgttatgaca aatcttcttc agttaaaaga
     1141 tacgatttgg gcattgatct tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg
     1201 cacattcttt aaacgttcgg tctctgattc gattattgaa tcggcacgga tcgatggagc
     1261 tagcgaactg cgaatatttg ttcagatcgt tttaccgtta gctgttccag gtattgcgac
     1321 aatcagttta tttgccgcat tgggttactg gaatgactgg ttcaatgctt tactatacat
     1381 ccaagatgat aagctagttc cactacagta tcttttgatg aaaatccaaa gtaatattca
     1441 atatctgaca caaaattcag gagcaggaag ccaattgaca ggcggattag cctcagttcc
     1501 aggagaatct gccagaatgg cgatcgtagt catctcgacg ttgcctattg cgatcagcta
     1561 tccatttttc cagaagcatt ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag
     1621 aaacaaaaat ataaatcata aaattaggag gaagaaaaat gaagttatgg aagaaactag
     1681 cctttacagg tgtttcagcg ttgttgttgg gaacattagc agcatgcgga ggttctggtt
     1741 ctaaagacca ggcagaagct agcaattcgg acactttgca gatgtatcaa atcggagata
     1801 aaccagataa tttcgatcaa ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg
     1861 gtgtaaaagt caatatcaat tacattggct ggggcgatta cgaaaagaaa atgaacgtca
     1921 tcatttcttc cggggaaaat tatgatatcg cctttgcgaa caactatgtt tccaatgccc
     1981 aaaaaggagc ctttgctgat ttgactgaat tagcgcctaa gtacgctgaa aaagcttata
     2041 atgatttaga tgaagcatat attaaaggaa atctggtcaa tggaaaatta tatgctttcc
     2101 cagtcaatgg aaatgtcttt gctcaacaag tacttacttt taacaaagct ttactagata
     2161 aatacgatct ttcaattgat ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg
     2221 aattccatga aaaagaacca aacacggcag cttttgccat tggacaaggc ttcaaagtcc
     2281 aagggaactt tgattatcct ttagggaata cgttaccatt tgctattgat ctggatggag
     2341 atgattcgaa aatcatcaac caatacgata atgaaacgta tatcggactg ttacgtacaa
     2401 tgcatagctg gtacaaacaa ggactcattc caagtgacgc tgcaacgagc aataaggatt
     2461 atccactaga aggaaatact tggctaatgc gtggagaaac acaaggaccg tatgattatg
     2521 gcgatacaat tttgacaaat gcagcaaaac aagaacttgt ctcaaaagca attactgttc
     2581 cactaaaatc tacagctcaa gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa
     2641 ataaagaaaa atcagtagaa cttctagggc tgcttaacag tgatccagaa ttattgaatg
     2701 ggcttgtttg ggggatcgag ggagaagcat gggaaaaagt cgatggttca gatgataaaa
     2761 tcaaattgct agatggttat cagccaaata cacatatgtc tgcttggaat acaggaaaca
     2821 acaagattct atatacacaa gagtctatta cggatgacat gattgccaaa cgtgatcaat
     2881 cgattgctga tgcagaaact tcaccgattt taggattcag cttcaacact gacagtgtca
     2941 aaacagaatt aagtaatatt tctaatgtca tgaatcaata tttagatgga ttgaatacag
     3001 ggactgttga tccagatgaa acattaccga aattgaaaga cgctttggac aaggctggct
     3061 atgataaggt attgaaagaa atgcaaaaac aatatgatga gttccgacaa gaataataat
     3121 gaataaagac aaaggggttg tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg
     3181 gtgttcaaaa gccagtttct cggtattaag ctaaatatat gtaaaaatgt ggaaagccat
     3241 attcacaaaa tttttcttac tactcgaaac taagcggcta tttcacaaac tcttggtaaa
     3301 cggtgttcaa tcagctaacc ctcggtattc gctcacaaaa cgacaaaata tgaggaacta
     3361 tttttccgtt tgtttctcta ataatcaggt caaggtgctg attgcccaac ctttttttgt
     3421 ataatgagaa agtataaact taatgtttgg aggagaagat atgttaggga aggttttgga
     3481 agagttattt atccggatct gggtcattat aaaactaacc ttgtatttct ggatatatac
     3541 atttgccggc gggatcatct ttggattagg tgcggcatgg aaaacagtca atgaactatt
     3601 ttatttgtac ggattcgagt ataaagaaat cacaataaaa agaggctgga atatatataa
     3661 aagaaatttt ttaagaggca atctgctatt tagtttattt ttatctggta ctgctttatt
     3721 gagttacaat ttatatttat ctgttcaaat caaagggctc atttttttag ctattgattt
     3781 tattttactt tttgccttgc tctgcctatt tgctacttat caatacagta tgattttaga
     3841 tagtgaatat acgatctctg ttcctaatct gctaaagcta agttttattt cggttttttc
     3901 cagcttttcg tcttttctaa aaataattat tggtagcggt ataattttag gcgtgacttg
     3961 gcaattcaaa ggattgatcc tgtttggtgt cattggtctt ttgacggttt ggaatggtac
     4021 gatgacaaca cattggcgtg aagaattaga taaacagttg gaaagttatg aataagtttc
     4081 ggattctatt ttcaaaagga accttactaa atcgtctgtt gcggaaatac gcggttatca
     4141 tgatcggtgt aacgatgact gctaccgtta tttttagtat ccatacttgg gaacaaaatc
     4201 aaaaacaggc agaaaacatg acaagtgatg ccgttcaaag cacaagcaga atgctgaatg
     4261 ataaaacaac cttgagtagg atcatcaaaa accagcttgt agggaattcc gaaaagatcg
     4321 aaaatgtgac gacctactta acaaaaccca tagatcagta tctgatgtat gtatacgaac
     4381 agcaaaacag taccgatgaa ttagtttctt tcccaaatca aatcaaagat ctctacgcaa
     4441 attatgaaga actaagtgcg atatatatcg tgttgaatca actaccggag tattatgaat
     4501 cttctagaga aaacaaaggt ggggaaataa aaaaggggac accaaaatta ctagacgcct
     4561 tttatatcaa gttgcctatc acacaaggag gagctgagtc aggaagtatt tttattggtt
     4621 ttcaaaagaa agaactagat atgattttag aaaatctaac gtcattcaat ggactctctt
     4681 tatacatgat ttctggaacg acaaatcgac tatacacatt ccatgataat aaaatagcta
     4741 aagaaacgtt tgaaagacaa gaaatgatca tcacagagag tctgaagaaa aattcacaat
     4801 tgccgattga cgatctggaa aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt
     4861 atcgtatatt agctgtacta gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg
     4921 ccccgctgat cattggtatc attattttag acaatatact gcttatcttt ctttataaaa
     4981 cattcaaacg atattcacag caagtatcta tcgtcatgca ggccatggat caaacagcag
     5041 cagggaattt agagacgcgg atcgacataa caaacacgga atatgaactg agggaacttt
     5101 caatcggtat caacgaaatg ttggatagca tcaatcagtt tgtagaagac atctacaaat
     5161 tagaaatcaa acagcaagac gcacatatgc gggcgcttca agcacagatc aatccacact
     5221 ttttatataa tacattagaa tatatccgaa tgtatgcaat cagcgaagga agtgaagaat
     5281 tagcagatgt tgtgtatgcg ttttccgctt tgcttcgaaa caatacgaat caagagaaaa
     5341 cgatcacttt aaaagaagaa ctggactttt gcgaaaaata tgtctatttg tatcaaatgc
     5401 gttatcctaa tcgagtagcc tatcatttta tgatcgatcc tgatttggaa aagatcgaag
     5461 tacctaaatt tgtgatccag ccattagttg aaaattattt taaacacggt attgatttta
     5521 ctcgattcga taatgcgcta agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca
     5581 tcatcaaaga taatggaaaa gggatgactg aaaaacggct aaaacagatt gaagaaaaac
     5641 tgtctcatcc taaagtggaa cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt
     5701 tacgtgccag cttcggcagc agttattata tgtcgcttga gaataatgag acaggtggac
     5761 tgaccgtttc tattacattc aaggagggat gagcgtgtat caagtgttat tggtagatga
     5821 tgaatacatg attttgaatg ggttaaaaaa gataattgat tggcaaagtt taggctttca
     5881 aatagtagca acagcagaga atgccaaaga aggactagct gtacttgaac agcgacagat
     5941 tgatttagta gtaacggatg tgacaatgcc agaaataaat ggactggaat ttattgaagc
     6001 ggctcaaaaa gaacgtcata attttgaatt tatgatttta tcaggatacc aagaattcga
     6061 ttatttgaaa ggaggtatgc aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa
     6121 gttcgaattg gttgaaagcc tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca
     6181 aaaaaatcaa caagaaatct atcaagaact gttattcagt caatggttga atgaagaatt
     6241 ggatgaaata agtgaagaag aaataatagg acaaataagt gagaagcagc ataggatagt
     6301 attgattcaa ctggcaagaa ctcatgatac tcttatcaat agatggttgc aagaacggca
     6361 acaaatcttt tactatcaac gaaattacgg agaccgtgta ttgatcacgt tgttgttgga
     6421 ggcagatgaa gttgatggct tctgttgttt tgttcaagaa aactttcctg atcaggaatg
     6481 gctgatcagt atcggggaag agacagattc tttggacaaa atccctgaaa cattccaaca
     6541 agcgaaagac aatctacagt tgcatcaatt ttatggagat aaagacgagc atgtttttta
     6601 cgcagtacaa gcagaaacaa aagatcaaac catcgatttt tcaagtttca gacgcgtgct
     6661 gcaaaataaa caattggaag tcgctcaaaa aatgatcact gatttttttg aacagtttca
     6721 gttagcagcc atgatgccgg aagatatacg atattccagt tttttactgt ttatggagat
     6781 tcaacgggaa ttgattgatc tggaagatga ggaatattta caaggaatcg aaaaaatcca
     6841 tcaggcaaaa acggtgcaag aacttcagca gctacttctg tcattcatca aaaagcatca
     6901 gagacagaaa aagtacagtg acaatgtgga aaaagtaatc gtaatcctgc accagcatta
     6961 tcaagaacca cttacactca aagaagtgtc agaaaacctg catttaaatg tcatgtatct
     7021 aggacagctg ttcaaaaaag aaacgaaaaa aagtttttca gcttatttga accatttacg
     7081 tatggaaaaa gcgaaacaac tgcttttgca cagtaatcaa aacatcaatg agatagcaag
     7141 tgagattggc tacaacaaca ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag
     7201 atcccctaaa gagtaccgag aaaacagtgg agaatcataa aagaaaaagc caatgtaatg
     7261 aaataactta taaaaaattg tctagaaatc taattttggg ctgttcttat tacttgaggc
     7321 aaggcagctt tttcatgacc tctcgataaa cggagttcaa aagccagttt ctcggtatta
     7381 agccaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt aatactcgaa
     7441 actaaacagc tttttcacga cctctccact aaacggtgtt caatcagctg accctcggta
     7501 ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc atttgtctct ctaatactca
     7561 ggtcagaacg ctgatttcac aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg
     7621 taataagacc aaccaaacga aaaatatgag gatctatttt tgcttggctg ttcttattac
     7681 ttgatgcagg acagcttttt cacaacctct tgttaattat agtttgataa ggaaaaaggc
     7741 aaatcttttg cttgatattt cttagggtga gggatgatcc ctaattggta agagatcgtg
     7801 ccgcctttca gcaaatcttg atgggtaaaa tataatttcg tatattctct gtccatccgt
     7861 ttgattttat ggataaattg ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc
     7921 tttccatttg acaaaagcaa aacagccttg tcaattaatg gcataccgat aacatattca
     7981 ccggttccag gcgtgacagg ataaaatccc agactattga agatatacca agcggccata
     8041 cttccattgt cctcatctcc agggtaaccc attggagaat cgtcgaaggt ctgagtcatc
     8101 aactgtttaa gcagtggctg tgccatttca ggttgtccta tataactgaa caagtaagga
     8161 tagtgaaaac ttggctggtt tgaaatagct aattgtccaa attctatcgc ggccatctca
     8221 ctcatttcgt ggatctcaaa accatatcct cctgggtcaa acgttggggg ctgattgcac
     8281 agctcaatga gtttttcttt atatctctca ctgccaccat attttccgat cagtcctgaa
     8341 aaatcgtgat aaacagcaaa gctgctttgc caagcacttc cttctgcata atcttgtccc
     8401 caacgattag ctgaaaatgg ttctctgaac ttgccatctt tatcttttga acgcataaat
     8461 cctgactcat agtcaaagat attttgatag ttcaatgatt gctgctgata tttcttcgtc
     8521 atttcctctt cttctagtac cttggcaact tggctaatac aaaaatcgct ataagcatag
     8581 tctaatgtgt gattgactga ttcgtggtag tgattaggaa catagccata tgtcaagtaa
     8641 tcttctgttc cttggcgtcc ataatttgga tgtttgcttt gaaccgtagc acctttttcc
     8701 attgccgtta gaaattcagg catcaagtct cgtcggatat cttttacagc agcatctgca
     8761 atgaccgcat cgatcaatgt cccaggcata agtccacgtt catcaggaga cagccatttt
     8821 ggcaaatagc cactttctct atagctgttc aaaaaacctt ccagcatttc ttcgtatttt
     8881 tctacagcaa tcaaagaata aagaggataa accgttttat acgtatccca aaacccatta
     8941 tttgtataga gaacaccagg ttttatagat cgactagtcg tatcatagtg gattttttgc
     9001 ccactaggat caatttcata aaatgtttgt ggaaataaaa acacccgata taaattatga
     9061 taaaacgtcc gcaattgttg ttgatcatgg tgtgtgagct gtatacgatc aaaataggac
     9121 tgccattgtt cttgactgtg agcaaggtaa gtctctttgc tttggttttg ttctagttcc
     9181 aggttataag ctgcttgttc cgagctgata aaagatgtcc caaatcggat agtttgtttt
     9241 tttgtctgtt caaaatacaa cgggatcgag tcgctatttt tgttggaaac agctaactca
     9301 tcaattgtta aaggatattc aaatctcaat ataaaataaa aagaaaaatc aggatcttct
     9361 gaaccagcaa aatgtgtgat ttttccttcc actgtgtaag gatctataac aaagagctga
     9421 tggcgtcccg gaaaggataa aagtaaaccg ctgtcatttt tgctgtaatc aatagataag
     9481 attccgccat acatgctagg tatcaatgta gaacagatgc cataacgtaa ttgacagatc
     9541 gacagatgag tcgggcaaaa tatactttct tctgggcggt aagaactttg agcatggaaa
     9601 agggtgttct cctgtagttt tccattgatc ggcgtaaaca gcatatgact gaaatcaccc
     9661 atccaaggac ttggttgatg ggtaagacgg tagccttgaa aaacgcggtc atcaggatga
     9721 aaccaccagc tgccgttttg atccgtagtt tgcggtgcaa aataattcat cccaaaagga
     9781 acgccagtat aaggctggca gttcccatta gagaaatttg gctgattcgc tgttgcatgt
     9841 ctagtatcta tttgaccgat attcatttta tttctccttt gttgattgtc ttttgatgaa
     9901 tgaaactgga aatttactat cggatggtcg atcttttgtc tctaaccaag tgaccaaagc
     9961 atctcccgca agcttaccta actgttcaaa atcttgtgcg atggttgtca gtggcgggtc
    10021 aacaagagct gctgcttgga tatcatcaaa tccgataatt gaaaaatctt cagggatatg
    10081 ataaccatgc tgtctcaaga gattcatggt ctggatagcg acaacatcgt tttcacaaac
    10141 aaaagcagtt acttcatact tagtgatata agaaagcaat tcatctaatg tcgcgctttt
    10201 gtcatccagt gaagtgtgga aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag
    10261 gtaacgctgt cgaacggatt gtggatgagt ctgcgtacca aacaaataag cgatttttga
    10321 atgtccctgt tcgatcaaat aagaggcagc aagcgcaccg ccttggaaat tatcggaaag
    10381 aacggtagga aaaggtaact catagatttt tttatctaat atgacgacag ggaattcttt
    10441 caatgaaagc tcaaaaagta agtcaagatg ttgttctgta tcaagagcat aataaatcaa
    10501 tccgtcaaat tcattgatta gctcttttgc tgttttattt tgtagaaagt caagcgtcgt
    10561 catcatgaca tcaatctgtt tatcttgcat cacagggttc aatcccttgc taaaatcacc
    10621 aacagacaaa tcagcgacat atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga
    10681 agcaagaatc tgttccggag aagcggattt aacgaaactg cctttcccgc gaacgcgata
    10741 aataagtcct aattgctcta attcagtcaa tgcacgtttg gaagtgattc tgcttacttt
    10801 ataggtacgt gaaagttctt tttcagttgg tagttggctg tctacaggta attcaccagt
    10861 taaaatagcc gtttgcaaat cattaaatat tgtctggtac aatggttgag tcatataaac
    10921 acctcttaat gatatatcat attcaaataa taccataatg gatgaaaaag taaataaaaa
    10981 gatatttttt gaaagcgtag acataattgg ctgtatgaag tataatcttt aaagatatat
    11041 caaatagaaa gaaggtttta ttagtggttt ataaagatat accaaaatcg gtgcagaaat
    11101 ttatggaaga tatcacacaa aaatgtgggg atatccatgc ggactgggca aagaatttta
    11161 cagcagcatt tgccaatact ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct
    11221 ttttattgac gggagacatt ccagcgatgt ggttaagaga ctctactgca caagttcgtc
    11281 cttatttagt gattgcaaaa gaagatgagg aattagctga catgatcagc gggttagtca
    11341 aaaagcaatt tttttatatc aatatcgatc catatgcgaa tgccttcaac gaagaagcta
    11401 acggtgctgg ccaccaaaca gatcatacaa aaatgaacga ttggatctgg gaaagaaaat
    11461 atgaaattga ttctttatgc tacccaatac agttggccta tcttctttac aaaaatacag
    11521 gaaaaactga tcaattcaat caatctttta ttgaaggtat caaaaaaatc ttggacgtgt
    11581 tcaaaacaga gcaagatcat gaacactctc cttatacatt tgtacgagac acgacaagaa
    11641 aagaagatac gctgatcaat gacgggcgag gtgcaaaaac agcttttaca ggaatgacat
    11701 ggtctggttt ccgtcctagc gatgatgctt gccgttatgg atatctggta ccatccaata
    11761 tgtttgcggt ggtcgtgtta ggttatgtgg aagaaatttt tcaaacgata ctcgatgata
    11821 aagaaattgt acagcaaaca cagaaattga agaaagagat ccaagagggg attcagaatc
    11881 acggacgaac ggttaatcaa aaagggcaaa cgatttatgc atatgaagta gatggaatgg
    11941 gaaacgccac agtcatggat gacagtaatg tgcccaatct gatcgccgca ccttacttgg
    12001 gatattgttc tgaacaagag gaagattatc tgcaaacaag aagaacattg ttgagtaaag
    12061 aaaatccata tttctatgaa ggaaagtacg caaaaggaat tggatcctcc catacgccag
    12121 aaaaatacgt ttggccgatt gcattagcta tggaaggaat gacaacgtca gacaaatctg
    12181 aaaaagagcg gatattgaat ttacttgtag caactgacgc aggaacacac cttatgcacg
    12241 aaggattcga tgtgaacaat cctgaaaatt atacgagaga atggttttct tgggcaaata
    12301 tgatgttttg cgaattagtc atggattact ttgatattag agtagaaaag taggaggtat
    12361 cgtaaatgaa aaagaaagta tatatcattt ctcatagcca ctgggatcgt gaatggtaca
    12421 tggcttacga acagcatcac atgcgactga ttgaattgat agacgatcta ctagagctat
    12481 ttgaagttga tccgtccttc aacagttttc atttggatgg gcaaacgatt attttagatg
    12541 actatcttca agtaaggcca gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa
    12601 aattgagaat tggaccattt tatatcttgc aagatgattt tcttatcagc tcggaatcga
    12661 acgtgagaaa tatgctgatt ggcatggaag aaagccgtaa atggggaacc cctgtcatgt
    12721 taggctattt tcccgataca tttggaaata tgggacagac gcctcaatta atgaaacaag
    12781 ctggtatttc agcagcagcg tttggccgtg gtgtaaaacc aattggcttt gataatcaag
    12841 ttttggaagc agaaaactat tcttcccaat actcagagat gtggtggaaa ggccctgatc
    12901 aaacagcaat ctttggtctg ctatttgcca attggtatag caacgggaat gagattccag
    12961 tagaaaaaga agcagctctt gccttttgga agcaaaaatt agcggatgca gaacaatatg
    13021 cctctacgaa ccacttattg atgatgaatg gtgttgatca tcaaccggta caaaaagata
    13081 tatcaaaagc aatccatctc gcaaatgaac tgtttccaga ttacgagttt attcattcta
    13141 attttactga ctatctggaa gcagttcaaa aagatgtgcc agaagactta ggctcagtag
    13201 aaggagaatt gacaagtcaa gaaacggatg gctggtatac tttagccaat acagcatctg
    13261 ctcgggttta cttaaaacaa tggaatacca aggtccagcg acagctagaa aatattacag
    13321 aaccacttgc gacgatggct tatgaagtta gtggaaacta tccccatgat caattagatt
    13381 atgcttggaa gacattaatg caaaatcatc ctcacgacag tatttgcggt tgttcagtcg
    13441 attctgtcca tcgggaaatg atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc
    13501 ttgcacaaga ttccttagaa cagctgaccg cagcaatcga tacgaccgga ttcccaaaag
    13561 acagctttcc atttgtcatt gtaaatactg caggaatgga taaaactggt gaagcagaaa
    13621 taaccatcga attagaaaga aaaagattcg ctgaaggaat acctgaacaa ctatatcaag
    13681 agttagaaaa tttgcctaaa cgtaagtatc atgtagaaac aaaaagtggg gccactatac
    13741 cagctatttt atctgaagaa actgtccaat ttggttatga tttgccgaaa gaccgtttcc
    13801 gtgtgccata tatggcaaga atgatcaaag tgactttacc attagaaaac atgccagcat
    13861 tttcttggga aacattcgcg cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa
    13921 tgattcatca gagtggacga atcatcgaaa acggccctct acatttgacg attgaaaaaa
    13981 atggtaccat tacaatggaa gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt
    14041 ttgaagatat cggcgatata ggaaatgagt atatctttaa acaacctttc tgtgatcagc
    14101 caatcctatc cagtaacaaa gaaaactctg aagtaaaagt attagtagat acaccggaaa
    14161 tagcaaaaat aagtattcta caggaaatgg aaatccctgt ttctgcagac gagcgtttag
    14221 aaaaagaaca gcagatggtt gtggaatttc gttatcgaaa agcagaacga tccaaagaaa
    14281 aacgaatcct tcaaataaaa acaatcatga caatcagaaa agatagtaaa aaaatcgact
    14341 ttgaaacgac tctcgacaat cagatgaaag atcatcgctt acgtgtcttg tttcctacaa
    14401 agttacatgt ggaacatcac gaagcagata gtatttttga agtggtgaag cgcccaaacc
    14461 atgtgtctaa aagttgggaa aaccccacga atccgcaaca tcagcaagca tttgtcaata
    14521 ttcatgatga agagtacggt gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa
    14581 cagaagacgg acaaatcgca gtgacgctac ttcgcagtgt gggagaacta ggcgattggg
    14641 gatattttcc aacaccagaa gcacagtgtc taggtgagca tcggttcaat tacagcattg
    14701 aactacatgg accagaagaa aaattctcta cttatttgca tgcatacgca gcacagattc
    14761 ctttttccac tcaacaaatc aaacatcatg aaggaacatt gatttcaaaa caacaatatt
    14821 taacgataaa aagtgaaacg tttgcaatta cagctttgaa acgcagcaag tttagtgata
    14881 aagtagtagt aagaggtttc aatatgtcta gtcatttaga aaaattggaa ataactaaag
    14941 acaatggaaa aactgtcata ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc
    15001 caataatcca accttatgag attcggacaa tcggattcga ggaggaaaac gaatgtatgg
    15061 cggaatagaa gcaggcggaa caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt
    15121 agaaaaatta agtatcccta ccacaacacc agaagaaaca ttggatcagg tatttgaatt
    15181 ttttgatcgt tatgaattga agggaatcgg gattggctca ttcggcccga ttggtatcga
    15241 caaaacaaaa gataattacg gatacgtttt ggcaactccc aaaaaagggt gggcacaatt
    15301 cgattttctt ggcacaatca aggaacgcta caatattcct gctgcttgga caacggatgt
    15361 caatgctgct gcatatgggg aattgaagca aggagcagca caaggaaaaa atagttgtat
    15421 ttaccttacc gtgggaacag gaatcggtgc aggtgtcgtg atcaatgaag aaattttttc
    15481 agggatcgca catcctgaaa tgggacatat ttgggtgaaa cgccatccag atgaccatta
    15541 tgaaggtact tgtccttatc ataaagattg tttagaagga ttagcagcag gaccgtctat
    15601 tgaggcacgc acagggatca aagggcaaga tctgccgcaa gatcatcctg tttgggctat
    15661 tcaggcttat tatattgctc aagcactgat caactatacg ttgacgttag cacctgaaaa
    15721 gattatttta ggcggtgggg taatgaatca ggatcatctc cttcaaaaaa tccgtcagca
    15781 atttgttgaa ttgatgggtg gttatatgga aacacctcaa gtaagtgaat atatcgttcg
    15841 ttggggactg ccgaatgaaa gtgggattat cggtagtttg ctgttagcag aacacgaaca
    15901 tcaaaagtaa gcagacaaat tacacaaaaa gaacagagtc ttatcattat ctgctgataa
    15961 gactctgttc ttttttattt ctattcccat aatccaggta aagctttacc gactattttt
    16021 tcttgagtaa atggatggat catgtgcagt tctaatgcat ggagcatcag tcgttcagct
    16081 gttctatttt ggtagagagg gtctccaacg atcggatgtc ccttatgagc taaatgaact
    16141 ctgatctggt gtgtacgtcc ggtttccaaa acacaataga cttgtgtttg ttcagaaagt
    16201 actttgttga cagtgacatg tgtcaaggcg ctctgaccat ttttatcatc tacgaccctt
    16261 tttctccgat catggcgatc tcgtccaatt ttattggtga tggtcatatc tttggcaatt
    16321 tctccccata cgatggcttg atatctccga tagatcattt tttgttccaa cattcttcca
    16381 agtatcggca agacaaatgg attttttgca aataaaacgg caccgcttgt ttctttgtcc
    16441 aagcggtgga caacgtaagg tcgttgctgt ttagtgtcga gataagcagc taaatgattg
    16501 agtaaggtac tgttttctgt cggttcattt ggatgagtct tcattcctgc aggcttatcc
    16561 actataatca aatgctcatc ttcatataaa acacggatgt gcttcgcatc tcctaattga
    16621 acttcttgat aattataatc tgtttcttcg aataacaatg tgatctggtc attgcccttt
    16681 acttcatgat ggaacaaagc aggttgttga ttgatccaaa cgtttttacg tatacgcaaa
    16741 aagtgtcgaa cctttctagg aactaaccat tccttttcaa gcaagtcgcg aattgttgtt
    16801 tcaggatggt tcataggtaa agtgatcgtg attttcacaa gtgtactcct ttcagtgcaa
    16861 ttgcagacta attgtaccat ttttaaaaca tatttaagaa aaaacttaca tttttctgtt
    16921 cattcttatg ttactctact tgcactaacg taaaaaaaca tattgaacta caacttaagc
    16981 aggaagaaaa cactctttca ctatggtaaa ataagacgat tgaatcaaat agacaggaga
    17041 agaagcaaat ggattttcaa agcattttag aaaagataaa agctgctttg atcagttttt
    17101 ggcgttggat cagaccatat ctcggacaat tccatcgatg gcgaaagcgc atctggaaaa
    17161 aatatcatgt gaacaaattg attttgctga ttggtttgat ctgtgtctta gtgacaagta
    17221 tctacctctt tattttagca aaacaagcaa atgtcgaaac gttaaaatca ggattaagcc
    17281 aatcgacagt catttatgat aagaaaaacg aagaagctgg cacactgtac gcccaaaaag
    17341 gaacatatgt agaattaaac gcgatttctc cctatatcca agatgcagta atctcgacag
    17401 aagataggaa tttttacgag catcacgggt ttgatatcaa agggattgcc cgtgcagcag
    17461 tccgaatggt cttgtctggc ggaacatcag ggggcggagg aagtacgatc actcagcagc
    17521 ttgccaaaaa tgcgtatctg acattagacc aaacgtttga tcgaaaagcg aaggaattat
    17581 ttttggcaat cgagatcgaa aagaagtaca gcaaagaaga aatcttgacc atgtatctta
    17641 acaatgccta ttttggcaac ggggtatggg gtgtccaaga cgcttccaga aagtattttg
    17701 gagtagatgc aacgaatgtt acgctgtctg aggctgctac tttggcagga atgctgaaag
    17761 gtcctggtat ttataatccg atcgatcacc caaaaaatgc gaatgatcgg aaaaacacag
    17821 tactaagtgt aatggaagaa aacggaaaaa tcacaaaaga acaagaacaa gcagaaagcc
    17881 agacagatat cagcgcgtat cttaatgaca catacgataa ttcagattct ggttatcgtt
    17941 atccttatta ttttgatgca gtgatcgatg aagcagttaa cgagtatggt cttgatgaag
    18001 aagatgtcat gaataaaggc tataaaatct atacagcttt agatcaggat tatcaaaagc
    18061 aattagaagc tacttatcaa aacagtgcat tattcccagc aaatgcttca gatggtgcca
    18121 tggtccaatc tgcttcagta gctttagatc cgaatacagg tggtgtacaa gcactagtag
    18181 gacgaagagg agatcatgtc tttagaggat tcagctttgc tacacagatg cgtcgttcac
    18241 caggatcaac gatcaaacca ttaagcgtat atacaccagc actggaagct ggatacaagc
    18301 cttccagtat cttgaaagac gaaccgcaaa gttattacga tgcgcataat tttgacggga
    18361 cataccaagg agaagtaccg atgtatcaag ctgttgcaca aagtttgaat ttgcctgctg
    18421 tatggctttt gcatgagatc ggtttacaaa aaggatatga taaagcagaa gaattcggcc
    18481 tgcctttagc aaaatctgat aagtattatg gcttagccct tggtggactg gaaaaaggag
    18541 tatctccatt gatcatggcg ggtgcttaca gtgcatttgc caatgatggt cagatgtata
    18601 cgccgcatct tatcacgaaa atcgttgatt cgactggagc ggtgatcgtc gataacacaa
    18661 acaaaaaacc aaaacaggtc atatcaaagg aaacagccga tgaaatgacc agcatgcttc
    18721 ttggtacatt ttcaaatgga acagcggctg ctgcgaatcc tgcaggttat acgatagcag
    18781 ggaaaacagg aaccacagaa actaatttcg atgccacaaa agcaaatgac caatggatga
    18841 tcggttacac gccagacgta gtgatttcga catggatggg ttacgaagaa tccagcaagc
    18901 tgcactattt ggaagggaca agtggaaatg ttgtcgggaa agtcttcaaa tcagcggcag
    18961 aaggcatcct tccgtatact tcaaaaacag gatttagtgt agcagatgcc tatgccacag
    19021 gaggacaagt ggtagctgca gatcaagttg gaaacacagg aaattctaat ggagagtctg
    19081 gaaattggca agataacttg aatcaatatg gtgaacaagc aaaagaaggg ctgaaaaact
    19141 tcggcgatat ggtgaaagaa ggcgttcagg gaattggtga agcggctaaa gacctctggc
    19201 ggaaatatca aggagagtag cccctttgaa aacgaataac cccatgttac aatagagagt
    19261 aactactaaa agaaaagagg gcataacatg tcaaacatct acgatagcgc gaatcaaatc
    19321 gaacgtgaga tccgtgaatt accagaattc aaagcgttga aagaagcgta cgagaaagta
    19381 caagcaaacg aagaagcaca caaactgttc aaagacttcc aagcgatgca aatcgaatta
    19441 caagaaaaac aaatgagtgg acaagagttc agcgacgaag atgcaatgaa agcacaagaa
    19501 atggctgtga aaatccaatc agaagaagta atcaatgact tgatgcaaaa agagcaaggc
    19561 ttcagtacaa tcatcaatga cttgaaccgt atcatcatga caccagttcg cgacctttac
    19621 agcgaataaa actattttat gaaacaacag agggtgtgaa agagacgttc ggccttgagc
    19681 gatgacaaaa ttgcttctta ttgccgatag gctgtctctt gaacaccatt taccaagagg
    19741 tcgtgagcct accgtttaac tctgagttac aaggaacaat ttcaaaaaac agcttctcat
    19801 gtttgatgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcaga
    19861 aataggagct acgacaaaag tcttgtcata gctcctattt tttgtcattc atttttcaag
    19921 cggtagaaat agcctttttg agctaacagt ttttcgtaag tacctatttc aacgattttt
    19981 ccttcatcga tcacaacgat tttatctgcc tctttgatcg ttgaaagccg atgagccaca
    20041 ataaaagtag tgggtgtttc tgtcaggtaa ttcagggctt gctggatttt tttctctgac
    20101 tggttatcta aagctgaggt tgcttcatct agcaaaatga tttttggctg acgtatcaac
    20161 gctcgtgcaa tggagataca ttgcttttgg ccaccagaca gcttgttgcc atgttcgcct
    20221 actaaggtat ccagaccatc aggaagctgg tcaatcagtg aagaaagttg cgcagcttcg
    20281 atgacttcat ctaaacgttc ttttgatacg tttttcaagc cataagtgat gttctctttg
    20341 atcgaagcgg agaacaacaa agtggtttga ggaacaacgg ctaaataatt tcgcacactt
    20401 cgcatatcca gttcatccaa aggctgatca tctagaaaaa tttttcctga agtcggctgg
    20461 ataaatccaa tcaataagtt aaggagtgta gattttccag agccagatgg cccaacaaaa
    20521 gcaattgttt ctccagcttt cacctctaga gaaaaatgat cgataatcgg tttgagtgag
    20581 tccggataaa gaaaagaaac atcctcaaaa cgaaaatttc catccaaatg ttggattttt
    20641 ttcttaccgt gataagtttc tacatcgcca gcaatcaaca gttcgccaat agaagtgata
    20701 gattccatgc cttttgaaat cgtaggaatc agcgtgatca ttccagaaac ggaattgacg
    20761 atcgtcgtga aatacgtctg atacatcacg acatcgccgg gttggatgcg cccttgtaaa
    20821 gctaatatcc cagtgaaaat caaacaaatc agctgaaata tctgaaaact agcccagctg
    20881 actgatccga aaacagattg caggatatct aagtggaatc ctttttcata gatttgagta
    20941 aaatgcttat ttaagcgagt tgtttcgtgt ttttctaacc catgagctcg agcaatcggt
    21001 gctaattcga tcacttcgat cacttttgca gaagtctctt ccatttcttg cctgaagtct
    21061 gagttggact gtctgatcct tttacggaaa aagagagtaa cgaatgcggc gagaggaaca
    21121 gtcaataaga aaaacatgaa aacaacagga cttttataaa gcgtgatacc taaagctacg
    21181 aataaattga cagcgatatt taatccgctg acaaatactt gagaggacaa cgtttggata
    21241 gcttctacat cacggatgat ctttgactgg attcgtccag attgcaattc tttttgataa
    21301 ggaatagata gttcttggat tttcttgata agggtcgctc ttagtccagc ttctacttga
    21361 cggatcgaag tgctatggta ttttacatga aaataattcg tcaatatatt ttgaccaatc
    21421 agacaagcca tcaaaaaagc gttgaaataa atggcctgca actgagaaga atctttcgat
    21481 gaagctgcat tgattacatt ggcaatagcg atcggcatta accaagatgg aagatgtttg
    21541 ataataaaaa agagtcctga catggcaaaa cgccaataat ttccctgata aagatgcagc
    21601 aatgtctgaa aagcatttgc atgatatttt tgataacttt tctgtattcg ttcctccata
    21661 atcttctcct taacaatctt ggtaaataaa ctgcaagtgt tctattgctt caggaacata
    21721 aatctcagga gtagcttcaa gagtggcgta cattagagga cgctcatatt tgatataatg
    21781 cagtaccggg cggaaatcta aacagccctt gccaacaggt accatgcgga tctttccatt
    21841 ttccatgata tagtctttga gatgaagcgc tgtaatataa ccatgtaaat tgtccaaggc
    21901 acctcggatc actttttcct gatcagcatg attttcttta tgcatcaaat tcgcacaatc
    21961 taaaatgatt tttaaatttg gtgaagctac ttcatcgatt aaacgtttag ctaactgata
    22021 gttatagatt ggatgattga tccctgcttc aacagcaaca gttacgccaa aattttctgc
    22081 atgagcgact aattcaatga tagaatttct ggcgatctga taagcttctt cagtaaaatt
    22141 ttcttttgtg taaccgacag agacactacc ggtttccgta gcaatcatct tggcttgata
    22201 ggcagagcac agagcaattt gctgtttgaa agttgccaat gcttgtgcac gaacttcttg
    22261 atttttgctg gataggttga tatagcatcc taaaatggaa agttcgatat tgtttttttg
    22321 gaagtattca ccaaaatagg cagcactccc aagtgtcatg tcagttaatt taagttgttc
    22381 aggaaatgct ttttgtggtg caaattggat atgggacaag tgaagctgtg atactttttg
    22441 aacgatttct tcacgatttc gaacatctaa atcgtgggca cggatgccta aatttaatgg
    22501 aaacatctca aactcctttt tgtaaataat tagcaagagg cagacgggat tcttttatca
    22561 attcaacaat tattcgggca actttatcag cgccttctgg actaaagtgg gtattgtctt
    22621 ttagtccttc tgggtaattt ttacaagtat caggcataag atgcaggtag aatttttttg
    22681 tctcttcttt tgagaaggtc tggaacaatt cttgtgatct tgagaacaca tctaaaagag
    22741 gaatatggtg tttctcagca aatgcacgca tcgcttttgg atagtctcca agtatatctg
    22801 gattcaatgt tccgttttct aagtaatctc gtcgcgtcac agatgtcagt aagaccattt
    22861 ggacgttcgc tttttttgcg cattcaaaaa atatctgcag attttttaaa tagctaccga
    22921 agggaagcgt acctctttct tcttggattt tctggtcgtt atgtccaaat tgtacaaata
    22981 agaaatcatt cggctgaaac tgtgaagcta gacgggctaa ccttccttcc tggataaatg
    23041 atttggtgct gcgtccattc tttgcttggt tattgatctg gatctcgggt gaaaaataag
    23101 cagagatttt ttctccccaa cctgtctcgg gacgcttgtt ttcatctttt tctgccgcag
    23161 tagaatcacc ggcaatatgg atagtaggca tgttatctct tctttcataa aggtttatag
    23221 gcaatgaagc agaacagcaa gagaagaaga cggagcaa
//
LOCUS       DAYYJC010000037        22208 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000037 DAYYJC010000000
VERSION     DAYYJC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.037"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..322
                     /locus_tag="ACHA9N_001381"
     CDS             <1..322
                     /locus_tag="ACHA9N_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9084850.1"
                     /translation="SSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIP
                     HWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEF
                     DYNI"
     gene            522..836
                     /locus_tag="ACHA9N_001382"
     CDS             522..836
                     /locus_tag="ACHA9N_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9084851.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            857..1234
                     /locus_tag="ACHA9N_001383"
     CDS             857..1234
                     /locus_tag="ACHA9N_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9084852.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1244..2017
                     /locus_tag="ACHA9N_001384"
     CDS             1244..2017
                     /locus_tag="ACHA9N_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084853.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2039..3442
                     /locus_tag="ACHA9N_001385"
     CDS             2039..3442
                     /locus_tag="ACHA9N_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9084854.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3624..4808
                     /gene="mobT"
                     /locus_tag="ACHA9N_001386"
     CDS             3624..4808
                     /gene="mobT"
                     /locus_tag="ACHA9N_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9084855.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4805..5095
                     /locus_tag="ACHA9N_001387"
     CDS             4805..5095
                     /locus_tag="ACHA9N_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084856.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5092..5313
                     /locus_tag="ACHA9N_001388"
     CDS             5092..5313
                     /locus_tag="ACHA9N_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084857.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5355..6134
                     /locus_tag="ACHA9N_001389"
     CDS             5355..6134
                     /locus_tag="ACHA9N_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM9084858.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6195..6695
                     /locus_tag="ACHA9N_001390"
     CDS             6195..6695
                     /locus_tag="ACHA9N_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9084859.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6751..7389
                     /locus_tag="ACHA9N_001391"
     CDS             6751..7389
                     /locus_tag="ACHA9N_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM9084860.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7461..7856
                     /locus_tag="ACHA9N_001392"
     CDS             7461..7856
                     /locus_tag="ACHA9N_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9084861.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7840..10293
                     /locus_tag="ACHA9N_001393"
     CDS             7840..10293
                     /locus_tag="ACHA9N_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9084862.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10290..12317
                     /locus_tag="ACHA9N_001394"
     CDS             10290..12317
                     /locus_tag="ACHA9N_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9084863.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12314..13336
                     /locus_tag="ACHA9N_001395"
     CDS             12314..13336
                     /locus_tag="ACHA9N_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9084864.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13353..14282
                     /locus_tag="ACHA9N_001396"
     CDS             13353..14282
                     /locus_tag="ACHA9N_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9084865.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14596..14643
                     /locus_tag="ACHA9N_001397"
                     /pseudo
     CDS             14596..14643
                     /locus_tag="ACHA9N_001397"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14659..15459
                     /locus_tag="ACHA9N_001398"
                     /pseudo
     CDS             14659..15459
                     /locus_tag="ACHA9N_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            15697..15933
                     /locus_tag="ACHA9N_001399"
     CDS             15697..15933
                     /locus_tag="ACHA9N_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084866.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15940..17892
                     /locus_tag="ACHA9N_001400"
     CDS             15940..17892
                     /locus_tag="ACHA9N_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9084867.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17964..18461)
                     /gene="dfrG"
                     /locus_tag="ACHA9N_001401"
     CDS             complement(17964..18461)
                     /gene="dfrG"
                     /locus_tag="ACHA9N_001401"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9084868.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18736..19863
                     /locus_tag="ACHA9N_001402"
                     /pseudo
     CDS             18736..19863
                     /locus_tag="ACHA9N_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            19961..20149
                     /locus_tag="ACHA9N_001403"
     CDS             19961..20149
                     /locus_tag="ACHA9N_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9084869.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20207..20560)
                     /locus_tag="ACHA9N_001404"
     CDS             complement(20207..20560)
                     /locus_tag="ACHA9N_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084870.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21091..21513
                     /locus_tag="ACHA9N_001405"
     CDS             21091..21513
                     /locus_tag="ACHA9N_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9084871.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21510..21740
                     /locus_tag="ACHA9N_001406"
     CDS             21510..21740
                     /locus_tag="ACHA9N_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084872.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         7553 a         3689 c         4283 g         6683 t
ORIGIN      
        1 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
       61 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      121 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      181 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      241 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      301 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      361 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      421 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      481 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      541 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      601 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      661 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      721 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
      781 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
      841 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
      901 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
      961 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1021 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1081 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1141 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1201 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1261 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1321 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1381 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1441 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1501 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1561 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1621 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1681 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1741 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     1801 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     1861 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     1921 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     1981 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2041 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2101 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2161 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2221 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2281 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2341 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2401 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2461 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2521 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2581 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2641 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2701 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     2761 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     2821 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     2881 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     2941 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3001 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3061 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3121 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3181 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3241 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3301 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3361 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3421 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3481 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3541 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3601 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3661 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3721 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     3781 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     3841 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     3901 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     3961 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4021 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4081 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4141 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4201 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4261 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4321 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4381 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4441 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4501 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4561 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4621 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4681 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4741 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     4801 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     4861 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     4921 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     4981 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5041 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5101 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5161 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5221 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5281 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5341 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5401 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5461 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5521 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5581 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5641 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5701 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     5761 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     5821 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     5881 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     5941 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6001 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6061 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6121 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6181 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6241 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6301 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6361 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6421 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6481 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6541 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6601 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6661 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6721 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     6781 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     6841 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     6901 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     6961 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7021 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7081 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7141 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7201 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7261 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7321 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7381 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7441 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7501 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7561 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7621 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7681 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7741 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     7801 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     7861 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     7921 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     7981 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8041 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8101 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8161 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8221 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8281 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8341 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8401 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8461 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8521 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8581 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8641 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8701 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     8761 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     8821 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     8881 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     8941 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9001 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9061 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9121 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9181 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9241 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9301 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9361 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9421 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9481 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9541 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9601 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9661 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9721 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
     9781 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
     9841 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
     9901 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
     9961 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10021 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10081 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10141 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10201 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10261 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10321 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10381 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10441 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10501 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10561 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10621 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10681 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10741 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    10801 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    10861 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    10921 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    10981 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11041 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11101 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11161 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11221 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11281 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11341 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11401 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11461 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11521 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11581 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11641 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11701 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    11761 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    11821 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    11881 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    11941 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12001 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12061 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12121 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12181 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12241 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12301 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12361 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12421 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12481 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12541 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12601 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12661 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12721 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    12781 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    12841 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    12901 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    12961 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13021 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13081 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13141 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13201 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13261 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13321 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13381 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13441 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13501 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13561 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13621 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13681 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13741 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    13801 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    13861 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    13921 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    13981 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14041 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14101 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14161 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14221 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14281 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14341 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14401 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14461 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14521 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14581 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14641 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14701 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    14761 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    14821 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    14881 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    14941 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15001 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15061 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15121 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15181 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15241 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15301 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15361 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15421 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15481 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15541 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15601 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15661 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15721 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    15781 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    15841 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    15901 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    15961 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16021 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16081 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16141 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16201 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16261 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16321 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16381 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16441 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16501 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16561 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16621 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16681 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16741 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    16801 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    16861 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    16921 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    16981 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17041 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17101 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17161 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17221 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17281 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17341 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17401 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17461 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17521 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17581 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17641 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17701 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    17761 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    17821 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    17881 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    17941 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18001 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18061 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18121 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18181 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18241 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18301 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18361 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18421 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18481 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18541 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18601 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18661 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18721 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    18781 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    18841 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    18901 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    18961 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19021 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19081 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19141 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19201 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19261 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19321 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19381 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19441 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19501 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19561 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19621 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19681 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19741 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    19801 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    19861 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    19921 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    19981 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20041 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20101 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20161 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20221 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20281 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20341 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20401 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20461 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20521 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20581 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20641 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20701 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    20761 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    20821 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    20881 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    20941 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21001 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21061 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21121 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21181 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21241 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21301 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21361 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21421 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21481 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21541 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21601 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21661 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21721 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    21781 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    21841 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    21901 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    21961 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22021 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22081 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22141 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22201 ggagtttt
//
LOCUS       DAYYJC010000038        22031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000038 DAYYJC010000000
VERSION     DAYYJC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.038"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACHA9N_001407"
     CDS             367..555
                     /locus_tag="ACHA9N_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084873.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACHA9N_001408"
     CDS             545..805
                     /locus_tag="ACHA9N_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFM9084874.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACHA9N_001409"
     CDS             846..1073
                     /locus_tag="ACHA9N_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084875.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACHA9N_001410"
     CDS             1138..1710
                     /locus_tag="ACHA9N_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084876.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACHA9N_001411"
     CDS             1711..2022
                     /locus_tag="ACHA9N_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9084877.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACHA9N_001412"
     CDS             2013..2273
                     /locus_tag="ACHA9N_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084878.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACHA9N_001413"
     CDS             2434..2793
                     /locus_tag="ACHA9N_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084879.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="ACHA9N_001414"
     CDS             2790..4433
                     /locus_tag="ACHA9N_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9084880.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="ACHA9N_001415"
     CDS             4468..5550
                     /locus_tag="ACHA9N_001415"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFM9084881.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="ACHA9N_001416"
     CDS             5594..6766
                     /locus_tag="ACHA9N_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9084882.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="ACHA9N_001417"
     CDS             6753..7457
                     /locus_tag="ACHA9N_001417"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9084883.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="ACHA9N_001418"
     CDS             7462..8622
                     /locus_tag="ACHA9N_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9084884.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="ACHA9N_001419"
     CDS             8698..8976
                     /locus_tag="ACHA9N_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFM9084885.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="ACHA9N_001420"
     CDS             8957..9328
                     /locus_tag="ACHA9N_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFM9084886.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="ACHA9N_001421"
     CDS             9344..9748
                     /locus_tag="ACHA9N_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084887.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="ACHA9N_001422"
     CDS             9745..10155
                     /locus_tag="ACHA9N_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084888.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="ACHA9N_001423"
     CDS             10216..10803
                     /locus_tag="ACHA9N_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9084889.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="ACHA9N_001424"
     CDS             10876..11343
                     /locus_tag="ACHA9N_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFM9084890.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="ACHA9N_001425"
     CDS             11389..11697
                     /locus_tag="ACHA9N_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084891.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="ACHA9N_001426"
     CDS             11892..18626
                     /locus_tag="ACHA9N_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9084892.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="ACHA9N_001427"
     CDS             18630..19502
                     /locus_tag="ACHA9N_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM9084893.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="ACHA9N_001428"
     CDS             19499..20548
                     /locus_tag="ACHA9N_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084894.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="ACHA9N_001429"
     CDS             20552..21376
                     /locus_tag="ACHA9N_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084895.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="ACHA9N_001430"
     CDS             21394..>22031
                     /locus_tag="ACHA9N_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9084896.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DAYYJC010000039        22028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000039 DAYYJC010000000
VERSION     DAYYJC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.039"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACHA9N_001431"
     CDS             complement(61..1371)
                     /locus_tag="ACHA9N_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9084897.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACHA9N_001432"
     CDS             complement(1383..2801)
                     /locus_tag="ACHA9N_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9084898.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACHA9N_001433"
     CDS             complement(3041..4609)
                     /locus_tag="ACHA9N_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9084899.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACHA9N_001434"
     CDS             complement(4613..4894)
                     /locus_tag="ACHA9N_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9084900.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACHA9N_001435"
     CDS             complement(4891..5814)
                     /locus_tag="ACHA9N_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9084901.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACHA9N_001436"
     CDS             complement(5811..6689)
                     /locus_tag="ACHA9N_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9084902.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACHA9N_001437"
     CDS             complement(6676..8013)
                     /locus_tag="ACHA9N_001437"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9084903.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACHA9N_001438"
     CDS             complement(8016..9119)
                     /locus_tag="ACHA9N_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9084904.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACHA9N_001439"
     CDS             complement(9193..10701)
                     /locus_tag="ACHA9N_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9084905.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACHA9N_001440"
     CDS             complement(10667..11359)
                     /locus_tag="ACHA9N_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9084906.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACHA9N_001441"
     CDS             complement(11381..12355)
                     /locus_tag="ACHA9N_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084907.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACHA9N_001442"
     CDS             12655..13527
                     /locus_tag="ACHA9N_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9084908.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ACHA9N_001443"
     CDS             complement(14109..16307)
                     /locus_tag="ACHA9N_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084909.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ACHA9N_001444"
     CDS             16539..17531
                     /locus_tag="ACHA9N_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9084910.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ACHA9N_001445"
     CDS             17591..19012
                     /locus_tag="ACHA9N_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9084911.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ACHA9N_001446"
     CDS             19005..20504
                     /locus_tag="ACHA9N_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9084912.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHA9N_001447"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ACHA9N_001447"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9084913.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAYYJC010000040        21988 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000040 DAYYJC010000000
VERSION     DAYYJC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.040"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA9N_001448"
     CDS             <1..395
                     /locus_tag="ACHA9N_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9084914.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACHA9N_001449"
     CDS             398..1147
                     /locus_tag="ACHA9N_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9084915.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACHA9N_001450"
     CDS             1159..1428
                     /locus_tag="ACHA9N_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084916.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACHA9N_001451"
     CDS             1433..1597
                     /locus_tag="ACHA9N_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084917.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACHA9N_001452"
     CDS             1590..1892
                     /locus_tag="ACHA9N_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084918.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACHA9N_001453"
     CDS             1889..2050
                     /locus_tag="ACHA9N_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9084919.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACHA9N_001454"
     CDS             2047..2352
                     /locus_tag="ACHA9N_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084920.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACHA9N_001455"
     CDS             2352..2708
                     /locus_tag="ACHA9N_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFM9084921.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACHA9N_001456"
     CDS             2668..2913
                     /locus_tag="ACHA9N_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084922.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACHA9N_001457"
     CDS             2910..3329
                     /locus_tag="ACHA9N_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9084923.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACHA9N_001458"
     CDS             3326..3883
                     /locus_tag="ACHA9N_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9084924.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACHA9N_001459"
     CDS             3880..4176
                     /locus_tag="ACHA9N_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084925.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACHA9N_001460"
     CDS             4253..4666
                     /locus_tag="ACHA9N_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9084926.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACHA9N_001461"
     CDS             5012..5386
                     /locus_tag="ACHA9N_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084927.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACHA9N_001462"
     CDS             5423..5590
                     /locus_tag="ACHA9N_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084928.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACHA9N_001463"
     CDS             5616..5960
                     /locus_tag="ACHA9N_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9084929.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACHA9N_001464"
     CDS             5965..6246
                     /locus_tag="ACHA9N_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084930.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACHA9N_001465"
     CDS             6349..6663
                     /locus_tag="ACHA9N_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9084931.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACHA9N_001466"
     CDS             6641..8335
                     /locus_tag="ACHA9N_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9084932.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACHA9N_001467"
     CDS             8355..9533
                     /locus_tag="ACHA9N_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9084933.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACHA9N_001468"
     CDS             9496..10182
                     /locus_tag="ACHA9N_001468"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9084934.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACHA9N_001469"
     CDS             10182..11342
                     /locus_tag="ACHA9N_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9084935.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACHA9N_001470"
     CDS             11352..12227
                     /locus_tag="ACHA9N_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084936.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACHA9N_001471"
     CDS             12224..12535
                     /locus_tag="ACHA9N_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084937.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACHA9N_001472"
     CDS             12525..12878
                     /locus_tag="ACHA9N_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9084938.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACHA9N_001473"
     CDS             12868..13269
                     /locus_tag="ACHA9N_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084939.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACHA9N_001474"
     CDS             13262..13666
                     /locus_tag="ACHA9N_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084940.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACHA9N_001475"
     CDS             13678..14286
                     /locus_tag="ACHA9N_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9084941.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACHA9N_001476"
     CDS             14306..14668
                     /locus_tag="ACHA9N_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084942.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACHA9N_001477"
     CDS             14671..14853
                     /locus_tag="ACHA9N_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084943.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACHA9N_001478"
     CDS             14870..18289
                     /locus_tag="ACHA9N_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9084944.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACHA9N_001479"
     CDS             18340..19077
                     /locus_tag="ACHA9N_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9084945.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACHA9N_001480"
     CDS             19087..21378
                     /locus_tag="ACHA9N_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9084946.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACHA9N_001481"
     CDS             21402..>21988
                     /locus_tag="ACHA9N_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9084947.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAYYJC010000041        21912 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000041 DAYYJC010000000
VERSION     DAYYJC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.041"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA9N_001482"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHA9N_001482"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9084948.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHA9N_001483"
     CDS             complement(1172..1867)
                     /locus_tag="ACHA9N_001483"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9084949.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHA9N_001484"
     CDS             complement(1880..2596)
                     /locus_tag="ACHA9N_001484"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9084950.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHA9N_001485"
     CDS             2926..3534
                     /locus_tag="ACHA9N_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9084951.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHA9N_001486"
     CDS             3859..4182
                     /locus_tag="ACHA9N_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9084952.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHA9N_001487"
     CDS             4364..5734
                     /locus_tag="ACHA9N_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9084953.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHA9N_001488"
     CDS             complement(5864..7165)
                     /locus_tag="ACHA9N_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9084954.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHA9N_001489"
     CDS             complement(7519..9510)
                     /locus_tag="ACHA9N_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9084955.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHA9N_001490"
     CDS             complement(9654..9920)
                     /locus_tag="ACHA9N_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9084956.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA9N_001491"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHA9N_001491"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9084957.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA9N_001492"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHA9N_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9084958.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHA9N_001493"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHA9N_001493"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9084959.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHA9N_001494"
     CDS             complement(14080..14601)
                     /locus_tag="ACHA9N_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9084960.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHA9N_001495"
     CDS             complement(14604..15446)
                     /locus_tag="ACHA9N_001495"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9084961.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHA9N_001496"
     CDS             complement(15439..16164)
                     /locus_tag="ACHA9N_001496"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9084962.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHA9N_001497"
     CDS             16340..16636
                     /locus_tag="ACHA9N_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084963.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHA9N_001498"
     CDS             17204..18796
                     /locus_tag="ACHA9N_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9084964.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHA9N_001499"
     CDS             18809..19549
                     /locus_tag="ACHA9N_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9084965.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACHA9N_001500"
     CDS             19717..21066
                     /locus_tag="ACHA9N_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9084966.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACHA9N_001501"
     CDS             complement(21194..21784)
                     /locus_tag="ACHA9N_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9084967.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DAYYJC010000042        21863 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000042 DAYYJC010000000
VERSION     DAYYJC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.042"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..1104)
                     /locus_tag="ACHA9N_001502"
     CDS             complement(205..1104)
                     /locus_tag="ACHA9N_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9084968.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="ACHA9N_001503"
     CDS             complement(1114..1470)
                     /locus_tag="ACHA9N_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9084969.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="ACHA9N_001504"
     CDS             1825..2121
                     /locus_tag="ACHA9N_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9084970.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="ACHA9N_001505"
     CDS             complement(2765..3718)
                     /locus_tag="ACHA9N_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9084971.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="ACHA9N_001506"
     CDS             complement(3741..4496)
                     /locus_tag="ACHA9N_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084972.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="ACHA9N_001507"
     CDS             complement(4493..5452)
                     /locus_tag="ACHA9N_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9084973.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="ACHA9N_001508"
     CDS             complement(5445..6395)
                     /locus_tag="ACHA9N_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9084974.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="ACHA9N_001509"
     CDS             6545..6997
                     /locus_tag="ACHA9N_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9084975.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHA9N_001510"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="ACHA9N_001510"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9084976.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHA9N_001511"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="ACHA9N_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9084977.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="ACHA9N_001512"
     CDS             complement(10549..11358)
                     /locus_tag="ACHA9N_001512"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9084978.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHA9N_001513"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="ACHA9N_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9084979.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHA9N_001514"
     CDS             complement(14968..15654)
                     /gene="rnc"
                     /locus_tag="ACHA9N_001514"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9084980.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="ACHA9N_001515"
     CDS             complement(16002..17783)
                     /locus_tag="ACHA9N_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9084981.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="ACHA9N_001516"
     CDS             complement(17809..18723)
                     /locus_tag="ACHA9N_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9084982.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHA9N_001517"
     CDS             complement(18750..19712)
                     /gene="opp4B"
                     /locus_tag="ACHA9N_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9084983.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="ACHA9N_001518"
     CDS             complement(19712..20656)
                     /locus_tag="ACHA9N_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084984.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="ACHA9N_001519"
     CDS             complement(20657..21661)
                     /locus_tag="ACHA9N_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9084985.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6119 a         4656 c         3862 g         7226 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaa
//
LOCUS       DAYYJC010000043        20630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000043 DAYYJC010000000
VERSION     DAYYJC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.043"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..1572)
                     /locus_tag="ACHA9N_001520"
     CDS             complement(109..1572)
                     /locus_tag="ACHA9N_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9084986.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1636..4653)
                     /locus_tag="ACHA9N_001521"
     CDS             complement(1636..4653)
                     /locus_tag="ACHA9N_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9084987.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4804..5643)
                     /locus_tag="ACHA9N_001522"
     CDS             complement(4804..5643)
                     /locus_tag="ACHA9N_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFM9084988.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5788..6033
                     /locus_tag="ACHA9N_001523"
     CDS             5788..6033
                     /locus_tag="ACHA9N_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084989.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6096..6518
                     /locus_tag="ACHA9N_001524"
     CDS             6096..6518
                     /locus_tag="ACHA9N_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084990.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6612..6929)
                     /locus_tag="ACHA9N_001525"
     CDS             complement(6612..6929)
                     /locus_tag="ACHA9N_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084991.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6956..7162)
                     /locus_tag="ACHA9N_001526"
     CDS             complement(6956..7162)
                     /locus_tag="ACHA9N_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084992.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7143..7325)
                     /locus_tag="ACHA9N_001527"
     CDS             complement(7143..7325)
                     /locus_tag="ACHA9N_001527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084993.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7339..7689)
                     /locus_tag="ACHA9N_001528"
     CDS             complement(7339..7689)
                     /locus_tag="ACHA9N_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084994.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7715..8017)
                     /locus_tag="ACHA9N_001529"
     CDS             complement(7715..8017)
                     /locus_tag="ACHA9N_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084995.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8020..8253)
                     /locus_tag="ACHA9N_001530"
     CDS             complement(8020..8253)
                     /locus_tag="ACHA9N_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084996.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9157..9687)
                     /locus_tag="ACHA9N_001531"
     CDS             complement(9157..9687)
                     /locus_tag="ACHA9N_001531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9084997.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(9684..10799)
                     /locus_tag="ACHA9N_001532"
     CDS             complement(9684..10799)
                     /locus_tag="ACHA9N_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9084998.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10805..11203)
                     /locus_tag="ACHA9N_001533"
     CDS             complement(10805..11203)
                     /locus_tag="ACHA9N_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9084999.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12232..12618)
                     /locus_tag="ACHA9N_001534"
     CDS             complement(12232..12618)
                     /locus_tag="ACHA9N_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9085000.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12615..13136)
                     /locus_tag="ACHA9N_001535"
     CDS             complement(12615..13136)
                     /locus_tag="ACHA9N_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9085001.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13138..14172)
                     /gene="sppA"
                     /locus_tag="ACHA9N_001536"
     CDS             complement(13138..14172)
                     /gene="sppA"
                     /locus_tag="ACHA9N_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9085002.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14205..15620)
                     /locus_tag="ACHA9N_001537"
     CDS             complement(14205..15620)
                     /locus_tag="ACHA9N_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFM9085003.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15589..17742)
                     /locus_tag="ACHA9N_001538"
     CDS             complement(15589..17742)
                     /locus_tag="ACHA9N_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFM9085004.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            18000..18236
                     /locus_tag="ACHA9N_001539"
     CDS             18000..18236
                     /locus_tag="ACHA9N_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFM9085005.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18255..18470
                     /locus_tag="ACHA9N_001540"
     CDS             18255..18470
                     /locus_tag="ACHA9N_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFM9085006.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19173..19925)
                     /locus_tag="ACHA9N_001541"
     CDS             complement(19173..19925)
                     /locus_tag="ACHA9N_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9085007.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(19940..>20630)
                     /locus_tag="ACHA9N_001542"
     CDS             complement(19940..>20630)
                     /locus_tag="ACHA9N_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9085008.1"
                     /translation="ITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKFARFRHDYKN
                     LLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLY
                     QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYRDD
                     KQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDP
                     YQFTTQVILLF"
BASE COUNT         5969 a         3980 c         3069 g         7612 t
ORIGIN      
        1 atacgagatt tcaaattaaa aaacgaaaat gagagataca aatatatcaa atatttaaaa
       61 agtaaccaca ttttctatac cactccttat cagaaacagc cttattcttt agaataagac
      121 tgtttctggg ttgattaatt tgtcttttgt taaactacca tctaaggcaa gttctaaatg
      181 taaatgtggt cctgtactat ttcctgttga acccacataa ccaataattt gtccttgttc
      241 aactgtttgt cctacgttca cgcaatattc ctgttggtgc gcataaagag ccacacttcc
      301 gtcttcatgc tcaattgtga cataatttcc tgacaaagta tgatacgaag cttctatgac
      361 cgttcctgcc ttgctcgcat agattggttc tccttgattg gctgccatgt ctaatcctct
      421 atgaaattct tctcctcgcc aaccgaagga actagaaatt gaataatttt ttagaggtac
      481 catatagcct tctttgctta gttgtggacc agctacttct ttatcatatt tgattaagtc
      541 ataggtttcg attaacccat ttaatttttt atcgtagcta gtgtctgttg cgtatcttcc
      601 tgttaaaaat ttagtcgctt cctgataggt ttttgcctta cttttccaaa caccatcata
      661 aaaagaggta tttccaacta agccttcttt taacagtttg gcatagtctt ttaagctttc
      721 ttcatagctc tcatattgcc gaaaagttgc ttgggtcgta taaagtgtcc cattgcctaa
      781 atcttcttgt gtcgcaaaag agacgccctt tccgttatgt gtgcctttga ttccaaatag
      841 attgtagttt ggtgcctgag ccaattgact ttgtccgctt gcggattcta aaatggcttg
      901 cgcgatcata acagaagcgt ataaatcatt ttcttgtcca attttacgag cagattcacc
      961 gattttacgg ataaacgttt ctacagacgc gtctttttca aaatgaatag tcccttcgtt
     1021 atcttgtgtg ctggaaggga ctaattcttg tatcggtgcg cttggtgctt ggtggtagtt
     1081 ttttgtctct tctgaaccga ttgattgtgc ctgcggtacg tcattcatag gtacagtcac
     1141 actctgttca ggtgcctttt gtgggcgttc cgtttttgtt ggcggttccg cttctgtttc
     1201 ggtacttggt ttttgaggag tctgtgagct ttcatcagaa ggttgctcat ttggttcttc
     1261 tgacggttcc tcctcaggtt gttgattgtc ttctggtttg tctggttgat tcggtgtttc
     1321 tggttgttct tcagaacctg ttggtggagc gttcatttct ggattatctg gtgtatctat
     1381 tgtatcttca gtcggtgttt ctggttgatt ttcttctcca gtggaatttg ttgtcgtttc
     1441 ttcagtgatc gttgcagatt gcgaatttgt cagttgattg ttgccttgtt cattagccaa
     1501 aaccgctggc gtatacccaa atacttgacc tactaataac aaagcagata aacatttact
     1561 cgtttttttc acaagattgc ctccattctt caatcaatca tctagagaca gctgattgct
     1621 gtctctagtc atactttaat tgctttgttt cattttacga cgaaccagaa cagcccccac
     1681 accaacaaaa agaaacagtc ccaatgcaat taacacatcg ttcactgttt ctcctgtttt
     1741 tggtaagctt tgttttggtg tgctcgtcgg cgtttgtttg acaggggttg tcggcgtttc
     1801 ttttttaatg ccttttgctg tcaatgtttg tttttcattc ttcaaatcaa agtgacgtgc
     1861 ttcttcctct ttatggtctt tatccttata gacaatatga gttgcgacca atttctcccc
     1921 gtcttttaac gtgcctgctt tgtagtcaaa ggatacttta aactcttcag acgctttaga
     1981 aactttacgt gcagtttcta ccgtaccaac gactgtatct tttccatttt tatcaatctt
     2041 atggaattga gtcacccagt atttggtttc ccctaccgta gattctggaa attcttgcga
     2101 tacatgatcg atcaactctt ggtctttgtc tggatcaaat gtttggtcgc cattcactgt
     2161 aataaagcgt gtttcgattt ttggttcttt cgcagtcact gtttgatctt tattggttaa
     2221 atcaaaatga cgagcgaatt cttcttcctc ttctttgtcg ttatagacaa tgtgcgttgc
     2281 gacaatcgtg tcaccgtctg aaaccatgtc tgctttgtag ttgaacgtca catcaaatgt
     2341 ttctttttca tcggtcactt ttcgtgtttc ttttaccgta tctaatacag tgacttttcc
     2401 atcggcagta attttatgga attgcgtcac ccaatatttc tctgtcccaa ccgcagattt
     2461 agggaatact tgggacactt tatccactaa ttcttggtct tttttcgggt tgaaatcttt
     2521 tccacctgtc gttgtcacaa acaaagtaga aatttctggt tggtcttctt ctggaaccac
     2581 gatttcttcg tttttcacag taattggttg tacttctccg tctttgaccg ttactaaaat
     2641 cgtttctgtt tgtcctgacg cttgcccata atgctcaggg aacacaattt ccgtaaattt
     2701 atacgtaccg tctggtaatt ttagtttttc tggttcttct tttgtttcat acacaaagga
     2761 cacattttta ttatgtgcag tcataccttc aatcaggaat ttcgctcctg gtaattgtag
     2821 atgattgatt gaatcgactt tattgaagtc tacttcaccc gtttttaaca agttgtcaat
     2881 cacatctaat tcaaacgtaa gcacaggcgt cgtgttatcg ccataggcca cagagaacgt
     2941 ggtttgttct tcagaaggtt gacggtagtt gtctggcaca tttttttcaa tcaattggta
     3001 tttgttttga tcgccattgt agaatttttt gtttgccttc cagtgtccgt ctttgtcagt
     3061 ttttactgaa tccaagactt ctcctttttt cacaaggact gtttctttat ctggcgcagt
     3121 aatatctgtt ttggcgataa tatcaaattc gatattttct gcaggaacgg tatcataaac
     3181 aggtttgcct tgattccagg attcttccgc attatagact tctttgactt tctttccttc
     3241 tggaatgaac tgttgtaagt cattttttgc agttaacgtc acttctttgt ttggttcaat
     3301 cgtaatcgta tgagtttgtt tgtctagaat atacccagct ggtgctttga cttctttgac
     3361 atccaattta ttttgaacat attttccgtc tttttcatag aatgcaggga tttcttttaa
     3421 ggcgatttcc cctttggcat ccgttgtata ttgttttgtt tctcccgtat catgacgttt
     3481 gatttcatac gtcacatttg gaactttttt ccctgtgaca gcgtctagtt tggttagttt
     3541 gaagtcgcca ttttttagaa ctttaatagt tagtccagct ctattaggat ctggcacata
     3601 aagctttcct agattttgag caacaggatc tacataggca aaacttgaac ctacccctct
     3661 agaatttgga tccagtgaag aaacagaaat tcgtccgtca tttgagtttt gatttgcttt
     3721 tataaacaaa ctatttcctg attttcttac ttgaacgtta gctgtgtttt caacaaccga
     3781 cccatcccat tgagaaaaca caccttttgt atccttaact tctacttctt cccctacttt
     3841 aaccgtcact gtttggctat caaaagaagg atcttcaagt aaattgttta tttttctatc
     3901 tacagtctgt ttgaaagctt cataattaga ttgtccaata gttccataat taacaaatga
     3961 accacctaaa acttcccaaa tatatgtttg tgctaataca tagttatcca ttgaaccact
     4021 agaatagtag ccaaaataac caattaaaga aatctttttt ctaatagcac ttgtcgtaat
     4081 taaattttcg agtggtgtca catcatattg aatacccgca ctcgtgattg cgccttcttg
     4141 aatgcaaaaa acatcttctc cgtcaatttg ccagcgaacc atgtaatcat gagtatacat
     4201 tgttccttca ggagtaattc gagtcatatt cacatcttta cgaccatttt cagtcaatgt
     4261 tagattggta gcacgtgtct gtgcattctt ttccaagatt gcttgccatt cttcagattc
     4321 ttgcgccaat cgttcttgtt cagaaaaaga agtcgctttt tgaatatctt caatggttat
     4381 aaagtatttc tcataacctt cagggacctc ttgagtcatt acattgggcg gagaaacatc
     4441 ttctgtcgtg tcctgcgtag ttgtctcttc tgttgtttca cttgtggttt cagtactgat
     4501 cgcttcttca ctactttttg attcttcagt cttcattact tcttcagtag aatttgattc
     4561 atttatcgtt tctgcaaatg ccgttacaga tcctaacatg ggattgagaa atgtaacgaa
     4621 aattgccaac caagttgcca ttcttttaaa catatttttt tcgttccttt cattttattt
     4681 ttcagtcttc taaagtaatt tcttatggac ttcgacaccg aaacattttt atcctcctca
     4741 aaacaaaaaa agactagaac ttataattct agtcttcatc aaccacttac ttttctttta
     4801 tttttagtag aaatctacag tgtgtttttg tgacccgtca ctgtaaataa taacatccac
     4861 cataaaacct ttgttgtacc actgcgaatt tggatcgcta acctctgcat tagcccaatc
     4921 ataggcttca tctacagtgt taaatacctt tccactatta ccaatctctc caactacaat
     4981 tgttggctct tcaggtgtcg ttgtgccacc atttccgcct tgatcgcctc caccaggatt
     5041 gcttggttct tcaggcgttg ttgttcctcc acctgtgctt gggttagaag gagttgtttc
     5101 tcctccagta tttccaccat tacctggttg tgtaggtgtt tgagtagtgc caccattgtt
     5161 ttctggtgta cttggcgttg ttgtaccacc agttgttcct gtttgatctc cagctggtgt
     5221 tgttggtgtc gttgtatttg gtgtcgtttg tgtatttgaa gtcgttcctt gtcctactgg
     5281 ttgattcgga tcaactttgt ctttctcttg aatcactgtt tgagccacag tttcacctgc
     5341 gctgttttgt acacttgcta cattgccatt aaatactaat ttatttcctg catagattaa
     5401 atccacattt gtaatcttat tcatgtccgc taatttttgg acagtaatat ttgttgcttg
     5461 actgattgcg cttagggtat ctccccagac aattgtgtat tcattccctt taatttctgc
     5521 tttgatttgt tccgccgaac gaggtgtcca aactttgttt tctgctgctt cagcattcgt
     5581 tcccacagca agactaactc ctccgactac gattcctagt gccaatactt tcaacatttt
     5641 cattgttttt cgcctccgat ttgaataaat ttatttacaa aaacatagta ccaaacttat
     5701 gtctatcagt cgatatattt tttgtagatt tttttatttt ttgatatact aactttggta
     5761 ctaaaataac taaaaggaat gtcacacatg caacttaata atttaaatga aatacatgat
     5821 tgggctcaaa ataatttact taatcgtaaa caagctgcca aaatcactgg acaaaactat
     5881 gatgcttttt ctagctcttt acgtagaaat aatatcaaac catttctaga aataggcgac
     5941 gaacctggtc cttccatcat tcgcttatat ctaagagaag atattgaaaa atacgcaaaa
     6001 cagctcgaag aaaaaagaca gaaacgtcaa tgaggcgttt tctgtcttgg atttacaagt
     6061 aaaacaataa taaacacttt tacaacggag gttctatgaa taagaaatac attgtttcag
     6121 ttggttcaat cgtactgatt gtactcgcta ttattgcttt ttctgtatcc aacaaaacag
     6181 aaacacagcc acaaaatacg gttcaatcga ctactacaag tcaatctagc gatcattccg
     6241 tctatccttc actaaatagt ttaaaagaaa cgcatggaca agatttaatt gatcttggtt
     6301 cttctcaaat tactactgtc ttatttgaaa agaaaaatat tgcgtttatt actcctaaag
     6361 aaggaactga ccttgaattt tctatcaaag taacaaatac caaagaaaat ttattctcca
     6421 ttcctgacat tcaatatacc cctaacaagt taaagattgg ggatacgttt ggtttggcta
     6481 aagtaaatga acaatacttt gcttactcac tgaaataagt ttctaatagc aagaggaaaa
     6541 accctcttgt tatttttatc acataatcat tttataaaaa cgcttcgaaa atatcaacat
     6601 ttttgaagaa actatttttc taatttctca atttcttcca agggattttc gctaatgacc
     6661 catacaggca cagattgctc tcgaggctcc ccttcttcta accaccaacc tgcataatga
     6721 acttcttcct cctcagcatt accatgatac caaccaacaa aggattcatc tacaacgaat
     6781 gaaatttctt cttctggatc aaataattca tttttccatg cttcaattgc ttgttctttg
     6841 gtgtactttt gtttgttaaa tacaccacaa atttcgtcat ctacataaaa aatttcaaaa
     6901 tcaaaattac tcgttccttt tactggcatc ttaacttttc ctcctttgac tcacttcaat
     6961 agatatacca ctcatgttca ttccattgtt tactagttaa ttggagtttt atcttagggc
     7021 tttgtgcact ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa aaaaggtaat
     7081 ttcttacatt cggtactaaa tgtttttcct gcatttcttc atgaaaaaca tataattctg
     7141 cttcattttt caaccaattc attattcatt ccctcattca cttcttcata ccgccattca
     7201 gatagtgaga tttctttttg tttctccact tgtcctataa aaatcgctgt attcaacaag
     7261 ctgatcacac ttaataataa agcaatgaaa ctaatggtta cacttactct ttcacatttt
     7321 gtcattttgt ttccttcttt actcgtttgt tatttcttgt aataggtcta ataactcctt
     7381 caccgaaatt tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac acattaccag
     7441 accttcaact gatatatcac tgcgacaata aaattctgga gaaacactga tttctgattc
     7501 tttcttacaa ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt cttccacaac
     7561 tatgttttgg gattctacca agaaagggag tgcctgttct aatgcttctt ctggtgtttg
     7621 agcagataga ataattgctt cttgttcttt tatttcattg ttctcaaata ctttataact
     7681 ttttttcatt gttagttcct ctttcattcc tctctcaatt agctaagccg ttactggtac
     7741 gccattctct aaatttagta tcgctaatcc ttaatgctcg tcgaatatca atcattcgat
     7801 aacctattgc acgaagttct ttatatcttt tagcagttag cgtctctaac gtcatttcag
     7861 gcaatgggtt cgaatcaaaa atagaattcg aaccgattat ttgggccaat tgttgtactt
     7921 gtaacagctc ttctgaactt aattcagtga ttgctcgatt gcctatcatt gcatacattt
     7981 tctgtcgaat atacttcttt tcttgtttag acaacatttc tatccactcc tattttcatt
     8041 atcatttgtt acctccactt cagtaaatcc acaaataaat gaattcatat tccaagtacc
     8101 attcccttca actacaactt ttctcctgtc ctctttcgga tattcaagaa tcagaccgtt
     8161 taccaatatg tttttcacta ctactaattt gtttgtatac cgtggaattg ctgttccgat
     8221 atacttcact ttatttccta cgctaattgt catttcctca ctcctattct tttttatcca
     8281 aaactttttc ctgtcctatt cctgccaacg aatatacttc actgcatgct taaacaaaac
     8341 aaaatgttgc cctgttttat ttccgtcttt tacttgttcc acatacagtt catatttggt
     8401 cacacctttt aaaacgccag atactttttt tcatccattg tcacaattag ttttttcatc
     8461 aatcgattga tactgcactg ctcatttttc ttgttcatat agtattcctt tctttttcaa
     8521 aactagcctc ttttgtttac tagttttcat caatcatcaa cgatttaaca gattttttct
     8581 ttcgcagggt ttctagcgtc agcaacccca aactctaaaa aagtgccttt gctcccttga
     8641 aaaacgagcg gacgattgta ctcgtcatca tatgtttttc atactacctg atccacgcaa
     8701 ataacctttt tttgttctgt gccacaccca tccatacgta tcgcttgaga ttcaccatac
     8761 ttttttaggt ggtttatcct tcctaagtcc ctgttagaca cgctgattaa cttagaaagt
     8821 cctgtttgta tccaattttc aaagatctcc caaaagaaat aagtaggttt acgtgtatcg
     8881 cttctgttca aagtacctgt ttgtacttga gtatttctac tcccctcgtt caagggattc
     8941 cttccaccag ctagcttttc ctttcatcat caaatgttcc tgaaaagcat ttacgtttcg
     9001 tgtccgtgtg cttacttatt cttttttcga tagcgattat ccgctcgcta ctcgctattt
     9061 tatactttgt ttgttagctc aaataaaaac taacaaacaa aaaaggctag tttctttccg
     9121 ttttattttt ttaactgtcg ataaatagtt gttagtttaa ttccaagttc taaaaccaat
     9181 acgcccaaca aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg gatagttttc
     9241 atgtcttccc tctttctttt tggtgtcgct gtggtcgttc ttgccgttca atacttcgat
     9301 acatttttta ttcattattt gctcgttttg ttttcctctg gactgctttt cttgcttttt
     9361 gaatacttta tacgcttttc gtgcctgaat taataggtct tgtcggcgtt tcattcgtga
     9421 gaaaacaata tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg caataatttt
     9481 cccatcaaaa aaaatagttt tcacttgaga aaaaaacagt tggaaatctt gttttgttgc
     9541 taattttttt gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga ttcttcgaac
     9601 aatccgattc gataacttcc aacgttgccg attaaacaat gttacttcac aacaaatatg
     9661 acgtaagtga ttaggggtca ttttcatgtg tccaattcct ccaaccaatt atgcatcgaa
     9721 attttgggca attgtgacag cccataaatc tgcgtttcca ggtcttcata actacaagaa
     9781 cgcttggctt gaagtaatga atattctgct tgatctgata gttttgttgc cattggatct
     9841 agattcaatt ctcttacaca acattcccaa aaattcaata gcgctctaaa ataaaatcct
     9901 tttcgattct gaattgcttt tccttcatga cgagcaattt tttcattttg aatacaccgt
     9961 tgatacgttt tgcttaaatc ttctgcaaat gtttccccta aaattcgata ctcgcttcca
    10021 tcagataaac catgtgtttg attatacttt cgttgccgct tttctataat tcgtttactg
    10081 tcgtatatca tgtttagctg ctctttcgct tcacgccatt cagctgaact gcgaacaatc
    10141 catgtcaaaa catctttcgg aacaaacgta ctttcgctgt aatgagcaat aaactctttt
    10201 tgccaatcat cagccatttc aacaggacta gtcgtatcga tttttgtatc tagattttct
    10261 gtatctagat tgttagaaga attattaaat aattgtgcag aattttcaca tggatttttt
    10321 tcgtcaaaat catcgaaaat cgctaaatta tcaacagatt cctcctcttc ttttccttgt
    10381 gcagaatcta cactggttga gtcaacagga cctaatgaat acacatcatt ttctgtcgta
    10441 tctaattctc ctagatacaa ccgagctttg tttctttttc ctgtttgttg atcaaatccc
    10501 atatcctcta tttctagtag ctcatattct ttcaattctt ttttgattgt attaacagaa
    10561 cgtctgcaac aattcaatgc attggctaat tcttgttccg taaattcaaa ataaacaaaa
    10621 ccttgactat ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg aaaaaccata
    10681 tacgcgattt ttgctttatc agacaatgag cgatattttt cacttgtaat aaataccttt
    10741 ggaaattgat agtataattc attgtattta cgagttgcgg taaatctttt tgttgccatc
    10801 gttattacac ctcatcttgt ccatatatta agaaagaatt ttggtgttgg tagacacgaa
    10861 catcttccat tgtagcaatc aattctttta gataaattcg accgaattca attgttagta
    10921 agtcgtaatc ttctaattca tttttgattt cttcaatttc ttcttcagac acattgagta
    10981 aaatggctaa ttcttgattg gtataagaaa tatagacatg tttcttttca tcaatccgtt
    11041 tttcaattaa ggattcttct aagtactcat tcagtaagct ataggcgtat tttgcttgtg
    11101 cagtcatcgc ataatatttt gacgatttta taaagaatag ttttggcata tgaaaagttg
    11161 cttgtgtcag cacatctgtt gcgcgaaatt tgttttttac catatttttt cctcctagaa
    11221 aaacacgcaa aaaaagctct gtataaatcg aattacacag agtttcttct tgttgatatt
    11281 taatttctaa aatacgtttc gggtaaaaac taaacactta ctaatattta gattgtagaa
    11341 tcttcgcctt catttattca ttttaaagag aaaaagatca agtgaaatgt gcccaaaagt
    11401 ttgattctat taatttttaa acaccattca taatatattt cctataatgt aaaaagcgtc
    11461 gatacttatt aaagcatcga cgttattttt atagtaaatt tatcaaaatt tttgccaaca
    11521 ttttttccga tttttttccc tatttctatc tttccttatc tttcttagtt aatattattt
    11581 gatgttaata tatcactact aatattaaaa aacattgatt tatcatagga taaagagtga
    11641 tagtttaata cttaaagaga catattaatt ttatttaaaa attgacatta atgactctta
    11701 atcagcgggt cgcgggttcg agcccctcac ggcccattgg gtgccaaacc cacggaacag
    11761 gtaaatgttg gtgcgaaagc atttcgaaaa catttacgct gttctttttt tatttttcaa
    11821 aataactagg gctttttatt tttaactcaa agtccactgt agccctttgg actttgagtt
    11881 aaaaaagaag cataattatg aacagtaaag tttctaatat aacaataatt cgagaagctt
    11941 tgcaaaatca tgctgccaat gaactctcta aacatatagg attaaatcta agtactataa
    12001 aaggttaaaa agtgaagaat atttaataga aaaactaaat ttacttgctg ctttcccctt
    12061 ttccattaat acaacaatca gagagtgtct ttttttcgaa aaaattagaa taaataaaag
    12121 acacgtagtt tttttatcta catgtcttcc atcgactaca agttatcatt tctcaaaacg
    12181 tatttagctt ttaattgatt aatcgtttca cgttgtctac acattgccta attaagattt
    12241 tcttattaga agcatcaatc ccgcaataat acctaaaact ccaggaatta cccacaataa
    12301 tgttaaaaga aaaaatggaa taatagataa aatgagtaaa ctaataccat agaccactgt
    12361 tttttgattt tttcttacta aaaaacctat tatgaaaatc attatcgaaa aaactagggt
    12421 aattcctcca aataaaacaa cattcccgct tagattgact aacatatcta tatccttact
    12481 ggttagttct gtagtattta aagtagttga atcaatagaa gatagaaaca taccacccat
    12541 caatgctaaa agactacata aaatccctgt tattccacta attaaaatca aaataaactc
    12601 tgtttttctc ttattcattt aattctctcc agattaagtg ttttagaatt agaaattttt
    12661 tgaccatagt taatagcttc taatgaacga ataacaacca ctgacgtgtt agaaaaataa
    12721 tccccaatat gttgtttctt gggcgtgaaa gcagccacca aatacccaat ttgaaataca
    12781 aaatttttta atataaacct acatatacac tcacgaataa agatagtttg ccaacttagc
    12841 tctttttcct caaagctcac tactcttatc ccaaaaatca ttttcccaat tgtttgtcct
    12901 gaattcagct tagtcaaaag tacaaaatag gttaaataaa tagctaatga cgctatacca
    12961 taaaaagtaa taatggattt gtcagattcc agtcccatga ttttgtaaaa gagacctata
    13021 gtagcatggg tgatgatgct aatacaaatt aaatcaataa gaaaagcaaa aaatcgtaac
    13081 caaaatcccg caaatacata attaggcaaa gtttcaatgc cactcttctt attcatttta
    13141 tagccctcca tagtaataca ttggttttgg agtatcagaa aatccaagtt tttcaaaaac
    13201 tgaaattaca cgatctgatg tagttgcctt tagcccttgc cattcagcaa tcttattacc
    13261 taaccaagaa gaagcaaagc cagttgtgtc atttttatat tctataaccg tcgcatcagt
    13321 taatttcttc tcttttttta gggaatctaa tgcatcttca gagtaaccaa tttgatccac
    13381 caacccattt tctactgctt ggttaccatc ataaattctt ccatccgcaa gttctttcac
    13441 tgcattagta tccatatttc ttccttttgc tacaacattg acaaaacgat cataagcact
    13501 catcacaaat tcctgtataa ctttattctc ttcctctgaa ggtttgtgtt gaggtgacat
    13561 catatctttt aatgcaccac ttttatacgt cgcatcagtt acgccaagtt tttctaataa
    13621 gccagaataa tttaaaccag aaataatgac accaattgat ccagttgttg tttcttccgt
    13681 agcaaatatt ttatctgaac cagcagaaat gtagtaccct ccacttgccg ctgtattttt
    13741 aaatgcagta tagataggaa tatctagctt tttaattttt gccatttctt tagcaatctc
    13801 agcactttca tatattcctc ccccaggtga atttacttct aataacactc ccttaaccgc
    13861 tttgtcttct tgaattttct ttagctgtgt taaaaaattc tgatggttat attgttccct
    13921 actaaaaaga ttgctttcac ctgtatctgc aataactcca ttgacggata gtttgacaat
    13981 ctttttattt gatgcgcctt cttctaacgt ttcttctgtg atttcgttag atccgtaaaa
    14041 aatcttattt attcctgtta gggttgtatt tgatgcttcc tctttatctt ttcctggaat
    14101 tagcgcgacg ataatggata gtaacaataa tccgcacgct actcccacag caatccatct
    14161 tcttttattc atataaactc ctcctatatt cttagtaaaa ataattattt tttatttaga
    14221 aacacatcga caatttgttg ccaatatgtt ttttctccta caattaaaga caactctcca
    14281 gttaatccat aacgaaacgg aaacgattga gttgttttta acgttccttt cactgcataa
    14341 aaaacacctt tctcagtggc agtagaattt tctgaaattt catctaaagt tccgtcgatc
    14401 gtaatcggag ccccaccttt ttgatccaat ttaaaatgaa ccttcatccc ttctttaata
    14461 taggtacttt cattcgcagg taaaagtgct gtaaatttca tttttgattt ttcgtggatc
    14521 ggataaattt ctgcaagcac tgttcctttg gctagttctg cttgtccctt tgtttcttca
    14581 tttaaatgaa ccgttccaga tacaggagct tttagtattc cttttgaaag ttgatcgtcc
    14641 aaacttttga taactgactg gttcttctct tgtttttcat ttagctctgt aactgtttgt
    14701 tttacagttg ttattgcttg ttctagctct tgctttcctt tttccgtttg actaattctg
    14761 tcattctcag aagtagttgg tttaactaat ttagcttttt ctgactgata ctgttctact
    14821 tcttttttta gttgctcaat ttgttcattt atgcttgctg aaattgtcag tttcacttga
    14881 tttttttgat catcagaaac attatttagt tgttcttgcc aattctcata gtttgccatc
    14941 atttcttttg aaaaatcttt taatgattga ttattactcc aagctacctg tacttgttgc
    15001 cattcttgta atttactatc tgaactctca atttgttttt ctagttgtct ttttgtcttt
    15061 tcgtatactt ctaattgctt ttgatcattt aattcacttt gcttcaaagc gtagcggagt
    15121 gattcttttt ctgacaacat gctctttaat tgattactgt agccaaaact gtcatccgtt
    15181 gaaaacaaat tttttccttt acttatactt tcaataaata gctgagccat ctttatttgt
    15241 tgagtaatac gttggttttc ttgttctatc tgagcctttt cattctctat taaagaacaa
    15301 tccaatctaa ccaacacatc gtccttatga acaaattgat tttcttttag gaagttttcc
    15361 attattttag cttctaatgg cacttgtaac ttctctattt tttcggggtt caattgagcc
    15421 gttgtccgaa taacaacctc ttttttagca aaggttaaaa acaatccaat aatacataaa
    15481 aaaagaatta cagggtataa cacccaacgg tagaagctat gatgttgttt agtatagata
    15541 ctagaatgtt cgaaccaatt attattctgc ataaattgtt tccctctcct agatttccca
    15601 taatttttga tagataccat cggcaaaacg aagttcatcg tgcgttcctt tttctaccaa
    15661 atgcccttca tggagaacta aaatttgatc acatgctttt gctattggta aatgatgcgc
    15721 aataaaaata atcgtttttt cttttaaatt gaggagataa ttcataattg catgttctaa
    15781 caaggtatct aatccactag ttgcttcatc taaaattaat atgtccggat tttttaataa
    15841 agcccgtgct aaggctaatc gttgtcgttg ccctcctgaa agattatttc caccttcttc
    15901 caacaatgtc tcgtaacgta atggctgttg attaataaat tcatttagtt ggactatctc
    15961 acaaacttct attatttgtt cgaaacttgg tgggctatct aaaccgaata taagattctc
    16021 aatgattgtt ccactaaaga aaaatgactc ttgaggcaaa tatgtcactc gatcgcgtaa
    16081 tatctgaaaa ggaatgtcta agtgattaat gtttccataa tggatcgatc cttctgatgc
    16141 ttcataaaaa cgaacaaata actttgcaag agttgatttt ccagaaccac taactccaac
    16201 tatagccgtt ttactgtgcg aaggaattgt acatgaaatt tcgttcaacg ttggggcctt
    16261 catgctatac gagaatgaaa cgttttttac agtgatatct tgttgaaata attgttcaga
    16321 aagcatatat tcaaaagact ggttcgattt ttctgaatca atcgcgaaaa tttcatttaa
    16381 acgattattt gcaacttgag ccgtttgcat tttcacttgt aaattaataa tgttttgtaa
    16441 aggatctgtc aaaaatacta gtaaagcatt gtatgtgata agttgaccta gactaattct
    16501 attatccata acaaaataag aacctagcca taaaattaat gcactgctaa ttaattgaat
    16561 caaatgttta attccttgtt gaatattatc aagcgtaacc gtacgaaatg attttttcat
    16621 taatttaaca aattcatggt ctatacggtt atatactttc tcttcaccgc tatatgcttt
    16681 aattgtttct attcctttaa gactttctat gatactagaa tttacagctg caccggctgc
    16741 catttcttcc ctgttcgatt tttcatagct tttcacgaaa gcgaatattg caactacata
    16801 aaaaggcaaa gagcatagag tgattaaaaa caacgtacta ttctgaatag ctaacgtaac
    16861 cccaacaagc aacaccatac caatatctaa aaatattgat aacgtagcac ttgctaaagc
    16921 atcaataatc ttactggcat caagaaaacg agaaataatt tcccctgatt ttctcgtagc
    16981 aaaaaaattc attggtaaag ttaacacatg tttgaaatac tgaagcataa ttgccatact
    17041 cattcgctgt cctaaaataa taagaagata actacgacta tattcaaaca atacccgaaa
    17101 taaatagaca gcgatcaagc caatggaaat tatatttaaa gttgaacgtg cttgattagg
    17161 gatgaaaaaa tcaagaatac cttgaaaata atatgagctt cctatgccaa aaaacgtaat
    17221 gaaaatcgat gctaaaacga tgtggaatac taatcctttt tgtttccaaa ttataggcaa
    17281 aaaagaactt aatccagcta ctttttcttt aataggaata taagtttctc taggcatagg
    17341 taataaaaga atacctgtcc attctttttg aaacgcttca atcgtttttc tattttttcc
    17401 attagcggga tctgcaatcc atagtacgtt tcccttcgtt ttataaatta caacgtagtg
    17461 catatagctg ttatcaacca atatatgtgc aattaatggt aagataagat ctctttcttt
    17521 ccagatctca ctatctgctt gaatagccat acaatcaaaa gacaattttt caaatgtctt
    17581 tttcaatcca aatgctgaag taccctctaa atcagtccca gataggtccc gtaatttatg
    17641 aatcgggata tcagttttaa aatattttaa aatcatcgaa aggcaagcaa caccacaatc
    17701 tttttcatct tgttgctgca caaaaggaaa tgattttatc acttatctaa cacaactcct
    17761 tctaaaaaat ttttatagaa aaattctcaa tatagcttta atacagtgaa aatattgtct
    17821 cataattcca caaaaacttt gaaaacagtt cttgaacact aactttttca ttcctgaatc
    17881 aaatttattt tggaaatttt actattcaag aataaacttg ataagtttca ggaagatttt
    17941 tactgttaat caaatacgta tgttacagta gcaccgtaaa aaatattatt taaagaagcg
    18001 tgtttaaaca tgcaaaatgt aaaggaacta agtattgtgg aaatgcacaa actgttggtg
    18061 gaggcaataa tgcttgggga aaattggggc aagttgtagg tggacttacc acaggggcag
    18121 taggtggtgc aggtttagga acagcaattt gcggaccagc ttgcggtgtt gttggagggt
    18181 tatatggagc tgtcgctgga ggtgctgcag ctggctggga tgctcgaaaa aaataaaaaa
    18241 ggagaataag tagtatgcat attaaaaaca caaaaacaac atttatatta tcttcagaag
    18301 agttgaaaaa tattcaaggt gggagtgccg tgggagtttt aggcactact ttttctgggg
    18361 caacagcagg agtaaaactt tgttcagccg gaggacctta tgcaatagct gcttgtggtg
    18421 taggtggagc tttattaggt gctggattca gcatgtggac cggtgcgtaa acaattaaaa
    18481 aatatcaaac ctcaagattg gtttgtgtcg attacgaaca taggtatgtt ctttatcttt
    18541 tcaaaagttt ggaatggaca gaacttttta ctaaagcttc ttgtgctagt ttttataatt
    18601 atattaaatt tttatctttt attaaagtca gataaaaatt ctttggaaat gtaatggtag
    18661 agatattttt acaaaaggac ttgcagcgct gtagttggtg ctgcaggagg acctatttcg
    18721 gccgttacag gaggtattgg taatgtactt gtcggatgtg cattaaagcc tgttcctgca
    18781 agttaaattt ataaaaacag gaaagtaatt atctcaaatc ctatcaagta caatcatagg
    18841 attgcacttg acaggacaaa ttttttaact aaaaaatgaa aaacactttc taaaaattag
    18901 tagaatttgc caaatctttc gcatcttcca ccatacattt aattttaatt cccctttatg
    18961 gtacttaata aaaacaagaa aagaactatt catttggccc ttttttggtt ccagaccata
    19021 gaaatattcc tccagtaact aatattgctg ttatgcacgt aactaataac agctataaaa
    19081 attggtacaa ctttaatagg attcagaaaa atttgcacta gtgaaggaat ctattttatt
    19141 agaaaaatgc aatctcccta aagaaaaaac cactaaacag tgctttcttt caacttctcc
    19201 ttaatttttt gtgctttccc taaagtaaag tttctggcca actctgaatc aaaatatagt
    19261 gaacgttttt taaaatctat ttctttaacg ttaaagatat ttactaaaca cgctcgacta
    19321 attcttgtta acataggata tttcttttca agctcactaa ttcttccata aaattcgtac
    19381 tgcccatctt tagtacacaa tgataatcta tgtggtaatt ttgaagcttc tacaaaataa
    19441 atgtcattta tatcaaaagt aaatgtttga gaacctatgg aaaaaacaaa tgcttgattc
    19501 gtaacctgtt ttgttgcatc tatcctttct tgcgctaata acaataactc aacaatctcg
    19561 tttcgatact catctagtgt ttgatctttt gttacaaatc ctaacgtttc aactcttctt
    19621 tttattgtta cgggtaacat ctcatcatga gtagttgtaa aaatgatttt tgcttgaaca
    19681 tcatattttc tgattacttc tgctaattct ataccgttaa cttcatgatt taaatcaata
    19741 tctaagaaat atattccttg ctttggtcgg aattgtttga ggtatttttt aacttctaac
    19801 ggactttgtg ttttcaatac tatcttaaat actttatcgt gaaaaagaat gaaattatca
    19861 ataattcgtt ctaactgatt taattgtata atttgatctt cacataatat tattggataa
    19921 ctcataaatc ctccttccct caaaacaaca aaataacttg agtagtaaat tgatacggat
    19981 cgttttgata gttcacaaac atattgggat tctttttatt gatttcctct atactagata
    20041 aacctaaccc ttgatgtccc ttttttgttg ttattccttt tttcattaga gtatctatag
    20101 agagtagtat ctctctacaa ctattcgata tcgaaatttc tatctgttta tcatctcggt
    20161 aaatagctag agacagttct ctatcttgac tctctataga tgcttcaatt gcattatcga
    20221 gaataattcc aagcacccta atacagtcaa atattggaac aggaacttca ataatagtac
    20281 ttggacattc gaatttacaa caaatcttat tgttatttgc ttggtatagc ttcgctatca
    20341 acaaactttt caaatattca ttttttatat tttttaaata acgatattca aacttattgg
    20401 ttagataaga attagaatag atctctaatt cttttatttc tttttccaat atcgaatcat
    20461 ggttttgatt agcgatttct tttaaactca gcaataaatt tttatagtca tgtctaaatc
    20521 tggcaaattt ttcttgatct ttttccagtg aatcagtata tttttttaga taaaccaatt
    20581 cttttctttt caattgctgt tcatattgtt ctcgttgcct aatagttatt
//
LOCUS       DAYYJC010000044        20364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000044 DAYYJC010000000
VERSION     DAYYJC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.044"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..260
                     /locus_tag="ACHA9N_001543"
     CDS             135..260
                     /locus_tag="ACHA9N_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085009.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            285..1400
                     /locus_tag="ACHA9N_001544"
                     /pseudo
     CDS             285..1400
                     /locus_tag="ACHA9N_001544"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            1483..2379
                     /locus_tag="ACHA9N_001545"
                     /pseudo
     CDS             1483..2379
                     /locus_tag="ACHA9N_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            2395..3219
                     /locus_tag="ACHA9N_001546"
     CDS             2395..3219
                     /locus_tag="ACHA9N_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085010.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3251..4561
                     /locus_tag="ACHA9N_001547"
     CDS             3251..4561
                     /locus_tag="ACHA9N_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9085011.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4709..5968
                     /locus_tag="ACHA9N_001548"
     CDS             4709..5968
                     /locus_tag="ACHA9N_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9085012.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5978..6850
                     /locus_tag="ACHA9N_001549"
     CDS             5978..6850
                     /locus_tag="ACHA9N_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085013.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6862..7692
                     /locus_tag="ACHA9N_001550"
     CDS             6862..7692
                     /locus_tag="ACHA9N_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085014.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7732..9915
                     /locus_tag="ACHA9N_001551"
     CDS             7732..9915
                     /locus_tag="ACHA9N_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9085015.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9976..11196
                     /locus_tag="ACHA9N_001552"
     CDS             9976..11196
                     /locus_tag="ACHA9N_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9085016.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11193..12653
                     /gene="gtfA"
                     /locus_tag="ACHA9N_001553"
     CDS             11193..12653
                     /gene="gtfA"
                     /locus_tag="ACHA9N_001553"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9085017.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12730..13836
                     /locus_tag="ACHA9N_001554"
                     /pseudo
     CDS             12730..13836
                     /locus_tag="ACHA9N_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14158..14367
                     /locus_tag="ACHA9N_001555"
     CDS             14158..14367
                     /locus_tag="ACHA9N_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9085018.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14360..14509
                     /locus_tag="ACHA9N_001556"
     CDS             14360..14509
                     /locus_tag="ACHA9N_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9085019.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14663..15328
                     /locus_tag="ACHA9N_001557"
     CDS             14663..15328
                     /locus_tag="ACHA9N_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085020.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(15612..16697)
                     /gene="xerS"
                     /locus_tag="ACHA9N_001558"
     CDS             complement(15612..16697)
                     /gene="xerS"
                     /locus_tag="ACHA9N_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9085021.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17031..17264
                     /locus_tag="ACHA9N_001559"
     CDS             17031..17264
                     /locus_tag="ACHA9N_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9085022.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17261..17668
                     /locus_tag="ACHA9N_001560"
     CDS             17261..17668
                     /locus_tag="ACHA9N_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9085023.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18805..19323)
                     /locus_tag="ACHA9N_001561"
     CDS             complement(18805..19323)
                     /locus_tag="ACHA9N_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9085024.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            19690..19938
                     /locus_tag="ACHA9N_001562"
     CDS             19690..19938
                     /locus_tag="ACHA9N_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085025.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         6898 a         3141 c         3790 g         6535 t
ORIGIN      
        1 agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc
       61 tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa
      121 caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa
      181 ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa
      241 ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg
      301 aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat
      361 attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc
      421 actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta
      481 tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc
      541 ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca
      601 aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac
      661 tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac
      721 atattggaac agagactggc tactggccat ttatttatca aaatggggga gaagtatttg
      781 ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt
      841 atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg
      901 ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg
      961 caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta
     1021 agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa
     1081 aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa
     1141 tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg
     1201 cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc
     1261 gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta
     1321 ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg
     1381 cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt
     1441 tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt
     1501 ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta
     1561 ggattaattt tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa
     1621 acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca
     1681 gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct
     1741 agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg
     1801 tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg
     1861 atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata
     1921 ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt
     1981 gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa
     2041 attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt
     2101 ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt
     2161 atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc
     2221 atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac
     2281 aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca
     2341 attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa
     2401 aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg
     2461 gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa
     2521 attccaccta caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat
     2581 ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt
     2641 ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg
     2701 aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt
     2761 actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata
     2821 ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta
     2881 cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc
     2941 aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg
     3001 tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct
     3061 ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg
     3121 gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa
     3181 ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga
     3241 gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa
     3301 cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga
     3361 tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc
     3421 gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc
     3481 tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt
     3541 tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg
     3601 gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga
     3661 agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg
     3721 tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt
     3781 accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg
     3841 gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga
     3901 tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt
     3961 acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc
     4021 agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat
     4081 tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg
     4141 agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata
     4201 tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa
     4261 aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa
     4321 aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg
     4381 gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa
     4441 aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat
     4501 caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta
     4561 aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag
     4621 taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc
     4681 attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg
     4741 tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag
     4801 cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga
     4861 gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc
     4921 gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa
     4981 tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt
     5041 aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga
     5101 ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac
     5161 tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt
     5221 agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac
     5281 tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa
     5341 caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa
     5401 agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg
     5461 tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa
     5521 tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt
     5581 tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt
     5641 atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac
     5701 aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg
     5761 cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg cttttactga
     5821 tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac
     5881 aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt
     5941 taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa
     6001 tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta
     6061 ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa
     6121 ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa
     6181 atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg
     6241 tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg
     6301 gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt
     6361 taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct
     6421 tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat
     6481 tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga
     6541 agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta
     6601 cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt
     6661 tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt
     6721 acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt
     6781 agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt
     6841 tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg
     6901 cttttattat tagggggctt catgatttta gttcccttag tttcaacgct attcggttca
     6961 tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg
     7021 gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc
     7081 atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta
     7141 gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga
     7201 atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt
     7261 ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta
     7321 tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa
     7381 attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca
     7441 atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca
     7501 ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca atataattat
     7561 acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc
     7621 acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga
     7681 gcagtgaaat aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata
     7741 tttgacaatc acacacaaac atttcattta caaaataaaa aaattagcta tctattagga
     7801 atcgaagaaa ataattattt aacgcatctt tattttggaa aaaagattac gcactattct
     7861 ggtgggtatc gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa
     7921 gatcgtttat tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat
     7981 tttagagaac cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt
     8041 tatgatagct acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat
     8101 acggaagatg atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga
     8161 ttgactttac agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg
     8221 caattagtaa ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt
     8281 gattttccaa atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt
     8341 caaatgacta gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca
     8401 agcagccatc aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa
     8461 ggagaggcat atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa
     8521 gatccatttg accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa
     8581 ctaaaaactg gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt
     8641 ctgaatacgt tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac
     8701 tatcgtttaa aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt
     8761 aacgaagaaa aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt
     8821 gttttagatg atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg
     8881 gttgaaacag aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat
     8941 aacgggatga aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta
     9001 tttcgagcac atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga
     9061 aatcaatatg ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg
     9121 acggcgattt ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg
     9181 acagaagtct attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat
     9241 atgttagggt tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt
     9301 gaaagttgtt cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa
     9361 acttggacga gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc
     9421 ttagtgtatc caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca
     9481 ggaagagaaa cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat
     9541 gagctagatc taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc
     9601 ttttataaaa agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg
     9661 tttgaaggca atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc
     9721 ttttatttcc gagtattagc agaagctgcg catccattag tcacattgaa attagcagga
     9781 ttagcagaag aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg
     9841 aatctcggtt tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat
     9901 ttgaaacaga tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt
     9961 ccaaacgaag aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat
    10021 tgggaaaatg aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa
    10081 ggactaggcg gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc
    10141 atctttgaat ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag
    10201 tcgggcattg aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt
    10261 atgacggtag cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat
    10321 ggctattatt cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt
    10381 gaatggttta aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat
    10441 caattagtta cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca
    10501 cgttcaatta ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg
    10561 gcgacgcttt ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga
    10621 atgaccaaca tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag
    10681 tataaacggg cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa
    10741 cgaagtcggg ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc
    10801 tcaacttctg ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa
    10861 gctcagttaa ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga
    10921 aaaaatagtg tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa
    10981 aatgaacata ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta
    11041 aacgttacag caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc
    11101 aataattatg aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa
    11161 agtattgtat tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca
    11221 tcacctatgc agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac
    11281 atttacaagg tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc
    11341 gaggctttgc gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg
    11401 tggaagcatt aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc
    11461 gtgaatcaaa atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa
    11521 tgtttatccg tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt
    11581 taatctacaa gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga
    11641 cagaagaggt ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag
    11701 ttgtgaaaga atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa
    11761 ttcgattaga tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg
    11821 aaccagatat ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg
    11881 aattattacc agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt
    11941 attatatcta tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat
    12001 cagaacgttt agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata
    12061 cgcatgatgg aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt
    12121 acacctctga ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca
    12181 attacaataa tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata
    12241 atgaccgtag ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa
    12301 tttattacgt tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag
    12361 aagggcgtaa tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac
    12421 ggccagtgat tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg
    12481 atttggatgg aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta
    12541 aaaataaaaa cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta
    12601 tcattgagtc taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc
    12661 catagaacaa aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag
    12721 gactcataca tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata
    12781 aaattaggaa aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt
    12841 aaaaaactgt cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa
    12901 gaaagtttgc gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat
    12961 gtgtcatcga ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg
    13021 ttagaagaaa tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag
    13081 cgaaatcatc tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag
    13141 tgggcgactt tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg
    13201 gacgatacta cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc
    13261 aatcaactac cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat
    13321 taggactttg aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt
    13381 aaaaaaaaca ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt
    13441 ctacgagacg agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta
    13501 gtcactgttc aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc
    13561 tcttcaaaag aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag
    13621 cacatcaaac aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag
    13681 aatcaattga ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg
    13741 aaaacggaaa aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc
    13801 tttgaatgtt gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac
    13861 ttttggtttt cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc
    13921 agcacagttt tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt
    13981 ttatgcaaca ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt
    14041 aaggagcaag tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa
    14101 cgctactatt tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg
    14161 gtggttataa caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga
    14221 atcatacgac aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga
    14281 agattaggac tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta
    14341 gaggaggatt ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt
    14401 tactgctagt ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg
    14461 gttaaaacag ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag
    14521 aagcagaaac ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac
    14581 cttattatca agggaaagta gaagaaagaa catataaaaa tcggttatct acaatcaaaa
    14641 aaacttttct tatttcttta aaatggagtt aagagaaata cgtccaattg acattcaaaa
    14701 gtggcaaaat aatttattga gcaaatataa aaattcttat gtaagaaaca tgtatgggat
    14761 ttttcagatg tcattagatc gagcagttgt tttgggacta cttgaaaaaa atcctgcaaa
    14821 agttattgga aatgtgaaga aaaggaaaac tacagttgat ttttggacaa aagaagaatt
    14881 tacaaaagtt atttcaactt tttatattga tgatttgtac caacattttg gttttatttg
    14941 tatgtggtta ctttttatga ccgttatgcg tttgggagaa gccactgcat taacttggaa
    15001 agatattgat tttgaaaata aaaacttgcg tgtgaataaa acactctatt ataagagagc
    15061 caaaacctat gagtttgtag aacccgaaac ggcttctagt attcgtacaa tcgaactaga
    15121 cgacaataca attacctttt taaaagcttg gaaggagcgt caagtttcaa taggaggcat
    15181 gtcgtttgta ctttcttata atggaacacc tactcagaaa tatacaatcc gtcatattat
    15241 gagaagacat gcaacgatcg ttggtgttca tataatccgc atacacggat taaggcatag
    15301 tcatgcttct ttgcttatat cacaataaaa agatgcactt cgaattaaag aaaggttaaa
    15361 acatgctgat gtacaaacga ctttaggaac ttatggtcat ttgtatctaa attcattgag
    15421 agagataaca aatgatccca cagattatat ccaaactaat ttttctaaaa tcaaaccgac
    15481 acctatttac gataataaaa aataaatgat aaacaaaagt aggatcacac caaaagaaac
    15541 acactagaaa aactataata caggcttttc tagcgtgttg tttcttattt ccgcctattt
    15601 atacattata ctcataaagc atttaatgca tcgatcgtag ccgcattgtc tatatgggta
    15661 tataaagttg ttgtattggt acttgaatgt cccaattgtt gggcagttaa taccaatgaa
    15721 tttgtctgtt ggtagagtct agtagctaat gtatgacgca gtttgtgagg gctgacttga
    15781 attctaaagc cttttgaata ttttgctacc atccgttcga ttgcaatacc ggatatacgc
    15841 ttggcttctc cttggtaaat tgctagaaat aaagcttttt cgttttctgt tactttatat
    15901 cgttctttcc gaatagctag ataattagct aaatattcca ttgcgaatgc tgctatattg
    15961 acagcatctc ttttatttcc ttttctagtt actaccacat tattcttatc taaagatagg
    16021 tcatctaaat taatatttat aacctcggag agtctcaatc cagatcctaa aatcagtgca
    16081 ttgatcgcca ggtctctttc tacatctcgt tgataataga tcaaagctcg tttactcaaa
    16141 gtatttttat attcattggc aatataatct agataatttt gtgtatcctt atctagaaat
    16201 aattttttct caatggcatt ggcacgtgca gctaacgtag ttttatctgt gatggttgct
    16261 acttgatcca taggattatt gtctaagtat ggttttccta gaggattatt ggaacttttc
    16321 gaaagatatt taaatagaac ctttaatgcc gtgatatttc tattgatagt agaagtagat
    16381 agacctaagt tattattatc tttgagctta gtttttgttg tctctttttt tacattttct
    16441 tttgtgcgac gtttcaaatg agatttgtac aagtttaggt cataggcagt taactgtctt
    16501 atcgaggtga tagaagtatc aggaacgata gactcactca gaagccattc ataaaagagc
    16561 ttataatttt ttagatattc taaagtggtt cgaggtgatt gatctttatc ctcacgatct
    16621 tgaacaaaat cttttatata atcgggcata tgaagtatga ttgtatctat tttttgatat
    16681 atttctgtat ttttcatgtc attcatcctt tcaataagta ggggagaaga gtatctctat
    16741 aattggacta taccaattat agaatgtttt gtcaattggt atgctctaaa atttattaat
    16801 ttgtttcaaa aaaagcatat aaacaggtat acgtttaata atttaaaata aatggtatac
    16861 ataaataggg aaggaaatag aagggagcaa tctaaatttt ttgaattttc ttaattaatt
    16921 atatagaaag aacaatcttt ctatagagtt agacatgaga gggactatgc ttgatttaaa
    16981 atcaacaagc gtatatactg tatatacaaa gaaaaaggag ggagaagact atggcaatta
    17041 aagaaagaaa atttagggaa gtggggaact cagtagtatt aactatttca aaggagtttc
    17101 ttaaagaaaa tgggttaaat cccggagata cggttttttt agatgaagaa aaacttcgag
    17161 cggcaattac aaaaaaacaa gccttatctg atgaaattga tttagcaata tctcaagcat
    17221 taactgaata tgatactact tttagagagc ttgtcgataa atgagtattc attacctaga
    17281 agccaaagat attataaaaa tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc
    17341 ccttggtgta aaggatgcga atgctttaga aatgtgtgta gggcaacttc gagcctcagc
    17401 ttttggtgaa gaggtttatc cttcgattta tgaaaaagca gctatcctac ttatacaatt
    17461 gataaagaaa catccttttc acaatgcaaa taaacgtact gctttcttag ctacttttgt
    17521 ctttttgaaa atcaatggac agcttttagc tattgatcaa aaagatgctg taaatttagc
    17581 ggtatatatt gctacgtatg ataaggattt cgatcaacta aaatatgaag tgatcaacac
    17641 tataaagaca catacgcagc ccatttaagg agttactgta tgtgtcttta tagggttatt
    17701 agctgctata gcgaaataat tgaagtaaat tacaaatagc tataattcaa atccaactat
    17761 atagaaaacg taaattttct atatagttgg attaagataa cgactattta ttttatcata
    17821 tttttactat accctatact tttacttggt atatttatga ataatcgcaa atttacccct
    17881 atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt cttttattaa
    17941 atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata aaaataacag
    18001 ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt gcgtatgaac
    18061 ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat cggtactggg
    18121 attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta ctatttgatt
    18181 tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt atagaattta
    18241 tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc ctttgatggt
    18301 tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa caaaggctat
    18361 ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc ttcctaaccc
    18421 aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg ttgagattcc
    18481 atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa aaataaaaaa
    18541 atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca taatctcatt
    18601 ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa tctaacgatg
    18661 caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa ccggcagtta
    18721 gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc tcctttccac
    18781 aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc ttatctttgt
    18841 tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata cttcccatac
    18901 agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata gatcttcatc
    18961 actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa tttcctctaa
    19021 ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact ctcctaactg
    19081 attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt catcattttc
    19141 ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag caggtgttct
    19201 atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa attctaaaat
    19261 atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt tatatgttct
    19321 cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata atttcatcca
    19381 cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct aactcatatg
    19441 tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg tcagcaagga
    19501 gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc atatcatttt
    19561 cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac taccacttta
    19621 agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc attgttaaac
    19681 ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg ctctttcttc
    19741 gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac gttccttacg
    19801 gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa tttaattcta
    19861 aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca agatttacct
    19921 gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat ttataatact
    19981 acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca gatacacttg
    20041 caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa tcttccgaaa
    20101 tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt tggcaaaaaa
    20161 ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt tgacacggtg
    20221 tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct aaaaagtcta
    20281 tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca acttatatgt
    20341 tttagtgaac tacccccact tagc
//
LOCUS       DAYYJC010000045        19811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000045 DAYYJC010000000
VERSION     DAYYJC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.045"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA9N_001563"
     CDS             6..305
                     /locus_tag="ACHA9N_001563"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9085026.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA9N_001564"
     CDS             378..1739
                     /locus_tag="ACHA9N_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9085027.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA9N_001565"
     CDS             1761..2021
                     /locus_tag="ACHA9N_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085028.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA9N_001566"
     CDS             2035..2787
                     /locus_tag="ACHA9N_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9085029.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA9N_001567"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA9N_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085030.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA9N_001568"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA9N_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9085031.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA9N_001569"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA9N_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9085032.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA9N_001570"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA9N_001570"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9085033.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA9N_001571"
     CDS             7161..8558
                     /locus_tag="ACHA9N_001571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9085034.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA9N_001572"
     CDS             8639..9079
                     /locus_tag="ACHA9N_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9085035.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA9N_001573"
     CDS             9227..9553
                     /locus_tag="ACHA9N_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9085036.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA9N_001574"
     CDS             9554..10303
                     /locus_tag="ACHA9N_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9085037.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA9N_001575"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA9N_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9085038.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA9N_001576"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA9N_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085039.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA9N_001577"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACHA9N_001577"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9085040.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA9N_001578"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACHA9N_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9085041.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACHA9N_001579"
     CDS             14770..17436
                     /locus_tag="ACHA9N_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9085042.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACHA9N_001580"
     CDS             17800..18552
                     /locus_tag="ACHA9N_001580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9085043.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACHA9N_001581"
     CDS             complement(18574..19728)
                     /locus_tag="ACHA9N_001581"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9085044.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6326 a         3472 c         4058 g         5955 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat a
//
LOCUS       DAYYJC010000046        19759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000046 DAYYJC010000000
VERSION     DAYYJC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.046"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..606)
                     /locus_tag="ACHA9N_001582"
     CDS             complement(73..606)
                     /locus_tag="ACHA9N_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9085045.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(700..1878)
                     /gene="mutY"
                     /locus_tag="ACHA9N_001583"
     CDS             complement(700..1878)
                     /gene="mutY"
                     /locus_tag="ACHA9N_001583"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9085046.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(1871..2668)
                     /gene="recX"
                     /locus_tag="ACHA9N_001584"
     CDS             complement(1871..2668)
                     /gene="recX"
                     /locus_tag="ACHA9N_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9085047.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2775..4157
                     /gene="rlmD"
                     /locus_tag="ACHA9N_001585"
     CDS             2775..4157
                     /gene="rlmD"
                     /locus_tag="ACHA9N_001585"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9085048.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4331..4909)
                     /locus_tag="ACHA9N_001586"
     CDS             complement(4331..4909)
                     /locus_tag="ACHA9N_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9085049.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5231..5701
                     /locus_tag="ACHA9N_001587"
     CDS             5231..5701
                     /locus_tag="ACHA9N_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9085050.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5809..7035)
                     /locus_tag="ACHA9N_001588"
     CDS             complement(5809..7035)
                     /locus_tag="ACHA9N_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9085051.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7789..7989)
                     /locus_tag="ACHA9N_001589"
     CDS             complement(7789..7989)
                     /locus_tag="ACHA9N_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9085052.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8123..8731)
                     /locus_tag="ACHA9N_001590"
     CDS             complement(8123..8731)
                     /locus_tag="ACHA9N_001590"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9085053.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8816..9286)
                     /locus_tag="ACHA9N_001591"
     CDS             complement(8816..9286)
                     /locus_tag="ACHA9N_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9085054.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9334..10248)
                     /locus_tag="ACHA9N_001592"
     CDS             complement(9334..10248)
                     /locus_tag="ACHA9N_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9085055.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10394..11197
                     /locus_tag="ACHA9N_001593"
     CDS             10394..11197
                     /locus_tag="ACHA9N_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9085056.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11375..12322
                     /locus_tag="ACHA9N_001594"
     CDS             11375..12322
                     /locus_tag="ACHA9N_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9085057.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12390..12683)
                     /locus_tag="ACHA9N_001595"
     CDS             complement(12390..12683)
                     /locus_tag="ACHA9N_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9085058.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12711..13376)
                     /locus_tag="ACHA9N_001596"
     CDS             complement(12711..13376)
                     /locus_tag="ACHA9N_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9085059.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13516..14148)
                     /locus_tag="ACHA9N_001597"
     CDS             complement(13516..14148)
                     /locus_tag="ACHA9N_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9085060.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14155..15222)
                     /gene="liaS"
                     /locus_tag="ACHA9N_001598"
     CDS             complement(14155..15222)
                     /gene="liaS"
                     /locus_tag="ACHA9N_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9085061.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15219..15950)
                     /gene="liaF"
                     /locus_tag="ACHA9N_001599"
     CDS             complement(15219..15950)
                     /gene="liaF"
                     /locus_tag="ACHA9N_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9085062.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16119..16598)
                     /gene="greA"
                     /locus_tag="ACHA9N_001600"
     CDS             complement(16119..16598)
                     /gene="greA"
                     /locus_tag="ACHA9N_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9085063.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16734..17909)
                     /gene="mltG"
                     /locus_tag="ACHA9N_001601"
     CDS             complement(16734..17909)
                     /gene="mltG"
                     /locus_tag="ACHA9N_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9085064.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18113..19282
                     /locus_tag="ACHA9N_001602"
     CDS             18113..19282
                     /locus_tag="ACHA9N_001602"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9085065.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19549..>19759)
                     /locus_tag="ACHA9N_001603"
     CDS             complement(19549..>19759)
                     /locus_tag="ACHA9N_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085066.1"
                     /translation="LTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKIS
                     LFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         5964 a         3957 c         3152 g         6686 t
ORIGIN      
        1 tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact ggcttactgt
       61 tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa tatcaatata
      121 gccttccgag aacgggccat ctccattatt gatccagtca accagaatct tcacattttc
      181 ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca tctcatattc
      241 atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca agtcatagtc
      301 gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac aataatagga
      361 aacccccttt tctctgatca ttgctattac attgaaccag tattttttat gaaaataaac
      421 aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta atgtgtgatc
      481 attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc gccacgttcg
      541 atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt cttttggaac
      601 tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg ttcattacac
      661 cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat tcctattgtt
      721 tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa cgaccgaatc
      781 aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt gttctgctcc
      841 ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa aaatatgagt
      901 aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa atgggagatt
      961 ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg tttcccattc
     1021 ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg tccacatatt
     1081 cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat gattttgaag
     1141 agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta caggaaaact
     1201 cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag tctcacactt
     1261 tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt tgaattcacc
     1321 tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc tgctagaagc
     1381 ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca cgttgccgtc
     1441 aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc
     1501 tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat caaattctga
     1561 catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac caaggccctc
     1621 ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg taggaaacca
     1681 ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct gaagcatgat
     1741 ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt ttcttttttc
     1801 ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat ctgtccattt
     1861 ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg atcatatcca
     1921 aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt ttaggttcaa
     1981 accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca tattctattt
     2041 cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct tgagaaaagc
     2101 ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt tttccgtgat
     2161 tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct tcaaatgtat
     2221 attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc atctgctgcg
     2281 ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa ctttccccat
     2341 agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta tttcgatctt
     2401 cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat cgcaactgat
     2461 aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc ttcttgatct
     2521 cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt actaatacat
     2581 cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa gggcctttgc
     2641 ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata ttctctatca
     2701 taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca gaaaaaagaa
     2761 aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa
     2821 tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg atcatatttg
     2881 ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca actcctcgct
     2941 atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat
     3001 gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat caagctcaat
     3061 tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca gcaggctttc
     3121 gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat agaaacaaag
     3181 cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt ttatacgaag
     3241 caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct cgaacacaaa
     3301 aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc tatgatgaac
     3361 ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa actggggagt
     3421 tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg
     3481 agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag gcacaaaaaa
     3541 cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc attgaagaac
     3601 aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc aatcctcagc
     3661 agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct aatgaaacga
     3721 tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa caagccaaag
     3781 aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa
     3841 gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt ctgcctaaat
     3901 ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt acgggtttgg
     3961 ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt tatatctcct
     4021 gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc aagattgaat
     4081 atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga
     4141 cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat caagtaataa
     4201 gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt gaccgaagag
     4261 ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt gtcacagccc
     4321 tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc
     4381 tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga agtgtgatcg
     4441 tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa
     4501 aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg tagtaatccc
     4561 aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt cctggattaa
     4621 ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag ccgctgactc
     4681 ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata ctttgcggtt
     4741 cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt cctaggtatg
     4801 cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta aaaccgaaca
     4861 accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg tagaaacctc
     4921 cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat tatgctttta
     4981 taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata acagaagtag
     5041 acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt tcaaaaacat
     5101 cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt ttttcttact
     5161 tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat tatagaaagg
     5221 agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta taggaattgt
     5281 catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt actattcttt
     5341 gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga ccttactgta
     5401 tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa aacagcgaaa
     5461 caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt ctgctctatt
     5521 ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag ttattttctt
     5581 gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg acaatgtggc tcatgattcc
     5641 ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt ttttaaacta
     5701 agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc taagttagat
     5761 tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct agtacccact
     5821 tgttgttcca tactcattat tctggtcata accataacca ttttgttcct gtccagcgta
     5881 aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat cttctgtttg
     5941 ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat aggaagaata
     6001 atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat cttccgaatc
     6061 atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat cgcttgtttt
     6121 tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt ttcctaaaat
     6181 ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt gtttgattgt
     6241 ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt
     6301 cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc catctaaact
     6361 taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc gaccgatatc
     6421 gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc cattcaatgt
     6481 ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat tattcacttt
     6541 gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg ttacataatg
     6601 atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga caccaccaaa
     6661 tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg tataaatatc
     6721 tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta ctaccatcat
     6781 cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac ctaatagcaa
     6841 aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg aggatattcg
     6901 attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa atctgattcc
     6961 gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta atagaacttt
     7021 ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt ctcatataac
     7081 tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca tttttgtgta
     7141 acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa
     7201 gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat gtattttttc
     7261 cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg atcgcacata
     7321 cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg atttcacgac
     7381 aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt tatttccagc
     7441 tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca tcttttaatg
     7501 gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag aattgacatg
     7561 gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat ccaaaaactg
     7621 aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata attagttagg
     7681 acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt cataaataga
     7741 caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt ataatttagt
     7801 cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc
     7861 ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa atacgtcatc
     7921 tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt taaccgttcc
     7981 gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta
     8041 tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat tatttttaag
     8101 actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt acttcatccc
     8161 aattaataac attccagaat gctgcaatat aatctggtcg gacattttta tatttcaaat
     8221 aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc atcaatggag
     8281 aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca agccaagccc
     8341 atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat
     8401 caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca ccagcatttg
     8461 gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg ttacgtacag
     8521 ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct attgtttttt
     8581 cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa gtattgtgat
     8641 gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat gcgtcatatg
     8701 cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa
     8761 taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt
     8821 cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta aaaataaaca
     8881 acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt ttcgccacat
     8941 actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc aatccgtaag
     9001 aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta aaattcggtg
     9061 attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca gaacaaaacc
     9121 acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg agagcaaaac
     9181 taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt atctcccaat
     9241 atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc caacctacct
     9301 tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt tatttaaaaa
     9361 aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc
     9421 atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct cgcttagtag
     9481 ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat actgccaaat
     9541 atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt ccattttttc
     9601 atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga tcaccattat
     9661 cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga tccagtagat
     9721 atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca gtgtatcact
     9781 taacaaatgc aaataactag attttatatt caaactttca tctgcttctt gtcgtaataa
     9841 taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca tcaaatgact
     9901 atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa acaaggataa
     9961 tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct ggtagccata
    10021 cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag aaaaaataat
    10081 tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac tccctgacat
    10141 aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta ctccaaatag
    10201 tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt tatttatatt
    10261 ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt cagctgtttc
    10321 cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg acactactag
    10381 atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac gattcaaaga
    10441 cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt tagttttgag
    10501 tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca acattcaatt
    10561 ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg ggcagcttca
    10621 aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca catttgatcc
    10681 agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt ttgttagtgt
    10741 cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga cttctgcttt
    10801 gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa acttaacagg
    10861 taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa aagtcctgac
    10921 atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc tatttaccaa
    10981 cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc ttgattgttt
    11041 aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct tgatggtctt
    11101 tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta tttttgctca
    11161 agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa ctcgttattt
    11221 ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc cttttttatt
    11281 tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc
    11341 tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca tttatgcaga
    11401 tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg gttatatctt
    11461 taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg gctggaactc
    11521 agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat ctatctcagc
    11581 tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac tttcaaatgg
    11641 tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc ttgggtattc
    11701 tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag gttcatcggt
    11761 ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc atattggaaa
    11821 aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca atacatccgg
    11881 tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg aagttcttcc
    11941 tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg ggctctttga
    12001 tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg gccgtggtgc
    12061 ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt cctttgcagc
    12121 attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga aaatggacaa
    12181 tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga aattaatggc
    12241 tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa ccaatcaagt
    12301 aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa aaagagaagg
    12361 tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata ggttggctcc
    12421 tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca ttcatctgct
    12481 tcctcgataa aaaaaagtgt cccatccatt tccactttct ttacaggatg ttctggctgt
    12541 tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta gacttttcca
    12601 aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca cttttgtgct
    12661 tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt ctattgattc
    12721 atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag attatcattt
    12781 tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac tcttcgtatg
    12841 gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt tttgttgaaa
    12901 aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa ctctaggtaa
    12961 tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc tcgctcggga
    13021 tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc attttgggct
    13081 tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa gatactggct
    13141 tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc taatgatcga
    13201 agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt catatattca
    13261 ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc gatcagtgtt
    13321 cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg tttcaccgtt
    13381 ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa caaaagcaag
    13441 catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt ttgattatcc
    13501 tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag cggcttgtgt
    13561 acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta ctgttttcag
    13621 tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa tcagcattaa
    13681 aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc gccgactcat
    13741 tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac gttgcgttgc
    13801 ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc ctgctgcacc
    13861 tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga taatgatttt
    13921 agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca tttcttccat
    13981 gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat agccttgtcg
    14041 tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat aagaagagac
    14101 gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc ttatctatgc
    14161 ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa aactaatgat
    14221 ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc cataacttcc
    14281 cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta acaaaagatt
    14341 attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc ttaatgtatt
    14401 tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag atagttttac
    14461 atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct tcaataactg
    14521 ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat gaagaagcag
    14581 cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca actgcttgcg
    14641 aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca tcatcatcat
    14701 tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc gatgtctttc
    14761 taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc tcgttacaat
    14821 ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt ccttatcaat
    14881 atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca atttggattc
    14941 ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg
    15001 aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg ctaacaaatg
    15061 gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc aatgactttg
    15121 attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag ccgcaatccc
    15181 cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat cacctcgaca
    15241 tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc ataatcactg
    15301 ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa caagacattt
    15361 cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa gatccgtgtt
    15421 ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag cgtattgccc
    15481 aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca ttcatagacg
    15541 tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt attatgtgtt
    15601 tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc atttttggtt
    15661 aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc aaatacagcc
    15721 atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc
    15781 aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata tatattgaaa
    15841 ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca aacagcaaat
    15901 aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat cgctctttct
    15961 ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc tataggactt
    16021 tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat ctagagtgta
    16081 tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc tactttgacg
    16141 attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt tttacgtttg
    16201 ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa cggatctgct
    16261 tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc gatgaatgta
    16321 actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat gatttgagca
    16381 aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg ttcatctttc
    16441 gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat tttgattcgt
    16501 tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc taatttttct
    16561 ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc cttttttcga
    16621 taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt accacgcttt
    16681 tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt tttctaagaa
    16741 ttatttacat atttttccac taattccata tgttcttcat aagttttagc gaaataaaca
    16801 tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg ttctaatact
    16861 gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc ggtattggta
    16921 tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc tttgtgttca
    16981 cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt gattcggtta
    17041 aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt ttcaactaga
    17101 gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc tgaataatag
    17161 ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt aagcgaagta
    17221 ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta ttttgtgttc
    17281 tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc gttaaagaaa
    17341 gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac cttcgctacc
    17401 cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc atctgcaact
    17461 tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc aagagtcata
    17521 ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa ttttgtataa
    17581 taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc accaatctgc
    17641 ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga tttatctaaa
    17701 ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc gccaataatc
    17761 aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc ttttttacgc
    17821 agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc ttttttatct
    17881 ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca agttcgtaac
    17941 tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa tattctatca
    18001 aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca agatacaagc
    18061 tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta ttatgaaaaa
    18121 aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg tgttgtttag
    18181 ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata atgaacactc
    18241 tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta ccaatgaaca
    18301 acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg gctcaattta
    18361 cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga ataaactcac
    18421 gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa aagcattgac
    18481 tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata ctaagctggc
    18541 tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact tgctgtatat
    18601 gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg aagaagtcgt
    18661 tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt atgattcatt
    18721 gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt atgaaaaatt
    18781 attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt atgaccaagt
    18841 caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg attactttaa
    18901 accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta atatcagtat
    18961 gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac tagtttccaa
    19021 agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata gtggtggggt
    19081 atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg atacatctgc
    19141 tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa aagctgacaa
    19201 aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga tggaaggaac
    19261 tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt ctttgaatag
    19321 catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca aatctaaaaa
    19381 tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt gttcttcttt
    19441 ctgttctgtc caaattattc taagatttct ctttaccaac attatttgac aaagggccta
    19501 ttaaatctaa aatagtatct ttagattgct aaaatatgtg ctagcaaatc attcatggct
    19561 tgttcttatt tttaatttta tgcaataaaa aagaagaaca attagcgtga ttatagaaat
    19621 aaataatgag attttagaaa aaaatgtgta tgaatacctg attgttactt tatttattcc
    19681 actttcactt tttactgtaa caaaaggacc tccttgtttt agtactgtag cattttttga
    19741 attaacagta accgttaat
//
LOCUS       DAYYJC010000047        19359 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000047 DAYYJC010000000
VERSION     DAYYJC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.047"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(645..1148)
                     /locus_tag="ACHA9N_001604"
     CDS             complement(645..1148)
                     /locus_tag="ACHA9N_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9085067.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1507..2532
                     /locus_tag="ACHA9N_001605"
     CDS             1507..2532
                     /locus_tag="ACHA9N_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9085068.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2855..3733
                     /locus_tag="ACHA9N_001606"
     CDS             2855..3733
                     /locus_tag="ACHA9N_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFM9085069.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4209..4325
                     /locus_tag="ACHA9N_001607"
     CDS             4209..4325
                     /locus_tag="ACHA9N_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9085070.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5252..5590
                     /locus_tag="ACHA9N_001608"
     CDS             5252..5590
                     /locus_tag="ACHA9N_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085071.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5684..5914
                     /locus_tag="ACHA9N_001609"
     CDS             5684..5914
                     /locus_tag="ACHA9N_001609"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9085072.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6314..6685
                     /locus_tag="ACHA9N_001610"
     CDS             6314..6685
                     /locus_tag="ACHA9N_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9085073.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6959..7651)
                     /locus_tag="ACHA9N_001611"
     CDS             complement(6959..7651)
                     /locus_tag="ACHA9N_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9085074.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7654..8439)
                     /locus_tag="ACHA9N_001612"
     CDS             complement(7654..8439)
                     /locus_tag="ACHA9N_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9085075.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8676..10076
                     /gene="lacG"
                     /locus_tag="ACHA9N_001613"
     CDS             8676..10076
                     /gene="lacG"
                     /locus_tag="ACHA9N_001613"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9085076.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10101..10418
                     /locus_tag="ACHA9N_001614"
     CDS             10101..10418
                     /locus_tag="ACHA9N_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9085077.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10431..12104
                     /locus_tag="ACHA9N_001615"
     CDS             10431..12104
                     /locus_tag="ACHA9N_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9085078.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12118..13749
                     /locus_tag="ACHA9N_001616"
     CDS             12118..13749
                     /locus_tag="ACHA9N_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9085079.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14621..15811
                     /locus_tag="ACHA9N_001617"
     CDS             14621..15811
                     /locus_tag="ACHA9N_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFM9085080.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15945..18134
                     /locus_tag="ACHA9N_001618"
     CDS             15945..18134
                     /locus_tag="ACHA9N_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9085081.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            18131..>19359
                     /locus_tag="ACHA9N_001619"
     CDS             18131..>19359
                     /locus_tag="ACHA9N_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9085082.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         6378 a         3084 c         3824 g         6073 t
ORIGIN      
        1 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
       61 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
      121 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
      181 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      241 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      301 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      361 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      421 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      481 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      541 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      601 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      661 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      721 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      781 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      841 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      901 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      961 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
     1021 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
     1081 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1141 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1201 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1261 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1321 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1381 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1441 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1501 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1561 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1621 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1681 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1741 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1801 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1861 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1921 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1981 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     2041 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     2101 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2161 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2221 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2281 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2341 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2401 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2461 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2521 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2581 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2641 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2701 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2761 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2821 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2881 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2941 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     3001 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     3061 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3121 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3181 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3241 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3301 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3361 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3421 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3481 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3541 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3601 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3661 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3721 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3781 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3841 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3901 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3961 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     4021 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     4081 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4141 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4201 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4261 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4321 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa cgtttttcgg ctattttcaa
     4381 taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata
     4441 tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata
     4501 gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc
     4561 aattcccttt caacctctta ttttgaccag tagtattgat cactttacct tttaacacct
     4621 ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt
     4681 ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca
     4741 ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat
     4801 ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact
     4861 ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa
     4921 taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga
     4981 attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt
     5041 atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt
     5101 agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt
     5161 ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat
     5221 tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca
     5281 tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga
     5341 gactccatta gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat
     5401 acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga
     5461 gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga
     5521 cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt
     5581 gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat
     5641 gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg
     5701 aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa
     5761 agaattcaag agtcgctact caattaactc gactccgaca tttacagttt acagagaagg
     5821 atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga
     5881 agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg
     5941 acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc
     6001 agtataaaga gtatccagag tattttattt taacttttgt atatttagta agtagctaca
     6061 cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc ttaattagct
     6121 tattgattct ataatatttt tgatttatat aggaaattaa aaatattata gaatcaacac
     6181 tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa tttctcctgg
     6241 ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact ttgcggagta
     6301 tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca tcaatcaatc
     6361 ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca ttatttcttc
     6421 agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata aagacaaaat
     6481 tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa
     6541 tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag atacggaaac
     6601 gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc agcttggtgg
     6661 agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag tatatcaaaa
     6721 aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca ctatttttaa
     6781 tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat
     6841 acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt cactggcagt
     6901 tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt tgaatcgttt
     6961 aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg
     7021 ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg aatggatcaa
     7081 ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag taagcatctt
     7141 cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta agaaaaatat
     7201 ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc
     7261 agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg
     7321 caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca agcccttttg
     7381 tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt tctttttctt
     7441 ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt ttcaaaatct
     7501 ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa ccatcttttg
     7561 tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc aaaaacattg
     7621 ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg tattttcggt
     7681 tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct cccgcgagat
     7741 ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat aaaaatcatc
     7801 tcgattgaat tttttattat caacgagtag atatttctta cgggaattat ttagagcgat
     7861 ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt gaacgccatt
     7921 tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc
     7981 gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt ctggtccatc
     8041 tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa ttttcttgtt
     8101 cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc
     8161 ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa tctgcagagt
     8221 ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt gagcccctcc
     8281 atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc gaacggtcat
     8341 atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt ttttattgac
     8401 catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct ctaatttaag
     8461 gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt aaacatttag
     8521 ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt tatgattatt
     8581 tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac aatgcaatca gttagaaaga
     8641 gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca gaagatttta
     8701 tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag
     8761 gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc gatccggcaa
     8821 gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg
     8881 gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg aaaccaaatc
     8941 cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc
     9001 cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat ggagattttt
     9061 tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt catgaattca
     9121 aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc aatcaatatt
     9181 tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc gcctgtcttc
     9241 ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac gaacttccgg
     9301 gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac gcgccggaag
     9361 ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta gatgctacct
     9421 atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt gaagcaaatc
     9481 aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta
     9541 atgactactt agggatcaat cactaccaat gccattttgt caaagcttac gatggagaaa
     9601 atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa gtgaagggaa
     9661 tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg ctgatttatc
     9721 cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa
     9781 tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc attattatgg
     9841 atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag gcgataacag
     9901 ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc tcatggacaa
     9961 atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag aagcgttacc
    10021 caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt atttaataag
    10081 tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt tttgagatcg
    10141 ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa gtaagacaag
    10201 gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta acccttgcac
    10261 ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg gagatgggat
    10321 tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga gatctgattc
    10381 aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct atggatggaa
    10441 tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt aatagttatt
    10501 taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt tcaagcattt
    10561 tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa tctgtctcca
    10621 catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc gtttcagcaa
    10681 ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac aaagtaatca
    10741 acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc gtctctccga
    10801 ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct tcctttgtcg
    10861 ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt accattaaaa
    10921 tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt cctttctctt
    10981 tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc caaatctcat
    11041 ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac gggtacttag
    11101 gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt
    11161 cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc aatctgcaat
    11221 tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg aattttgtag
    11281 gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta ctctttgcta
    11341 agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt ttcgctgtca
    11401 acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt attccatttt
    11461 tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga
    11521 acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg gttctaggaa
    11581 cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt gacttcctga
    11641 tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag
    11701 cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg
    11761 cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg
    11821 gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag
    11881 caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg caggattttg
    11941 atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa gaagacaccg
    12001 atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga ttaacgagag
    12061 atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg agccataatg
    12121 aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg ggctttgggg
    12181 gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc agatatttcg
    12241 atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat ttcaaaagat
    12301 gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt gtggcaggat
    12361 ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga aacgacgatt
    12421 gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt tcactacagt
    12481 gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg tctgaccttt
    12541 gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga aatgaagcag
    12601 ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg
    12661 ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc tgcttttttg
    12721 aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt aatgctccac
    12781 ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat cacgagtcga
    12841 ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg ttcaatgaat
    12901 gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt
    12961 gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc ttttggtgaa
    13021 gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga gtatttgctg
    13081 gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt cttctattgg
    13141 gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat gaattatctc
    13201 agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga ttttgctgga
    13261 aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa
    13321 aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca aacctggttt
    13381 caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt tggggactac
    13441 aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa tgttagtggc
    13501 ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga gtttgatact
    13561 aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt tgttaaaaca
    13621 agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt ttgtgaaatt
    13681 ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt ttcattggtg
    13741 aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag ccacatcgtt
    13801 agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac cagaaacgat
    13861 ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact ttgtattatg
    13921 ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga
    13981 taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat atgatttgag
    14041 cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga acttcttttt
    14101 atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg aaccatctta
    14161 tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag ggattccccc
    14221 tccatattat gttggctctt tattcccact ctgttttcaa cgtaaagtgt ataaaacaga
    14281 atgcgatact atcaagggag ttaaaacaat atacagaaca gtaaaacact ataaataaaa
    14341 ataaaagtgc aataaaagtg caatttttgc acctaatcat actctgttta tatatgaaaa
    14401 ctagattctg aatccaatat ttggttaacg agaggcgttc gaatttgaac gtcttttata
    14461 tattactttc aaataaatat gaggtagaat actatctatt ttctttttaa cccaattgtt
    14521 ataatggaga gtgtaaaata ttttgtgtaa atagaaaaaa ggaagtccct tctgtagaat
    14581 agagttacca caacacattc acagaagaga ggacttccct atgaacgatt ttactacaga
    14641 aattctaaag actctagcga acaaaggcga tttgaatgaa ttattccgtg tccatttgga
    14701 gaaagcagtc aatacgcttc tcaaaacgga gttaacggct ttcctagatt acgaaaagta
    14761 tgatcgcatt ggttttaaca cgggtaattc tcgtaacggc tcctatgacc gtacggtcaa
    14821 gaccgagtat ggggaacttc atctccagat tccgcgcgac cgcaatggtg agttcaagca
    14881 acagactgtt cctgcttata gacggacgaa tgacacgtta gaggagaccg tcattcacct
    14941 cttccgaaaa ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca
    15001 ttactacacg ccccaaacca tgtccaatat aacaaaatca tttacagaag aggtaacggc
    15061 gtttaatggg cgggagcttc atgaccgtta tgctgctatt tatatggacg caacgtatat
    15121 tccgttaaag cggaaaaccg tcgccaagga agctattcat atcgcagttg gcattcgccc
    15181 ggacggatca aaggaagtat tgagctatgc gattgcaccg actgaatcca tcacgatttg
    15241 ggaggaaatt ttattggacc ttcaagagcg cggtttgaaa aatgtcctcc tgttcatcac
    15301 ggatggctta aaggggatgg taggagcgat tagtcggttc tatcccaaag ctcgttttca
    15361 acattgttgt gtacacgttt cccgtaatat cagtcataaa gtgcgtgtcg atgatcgtaa
    15421 ggaagtctgt gatgatttta aaatggtgta tcaagcctca tctaaagagg tggcgttgga
    15481 agcacgtggt acttttgcgg aaaaatggaa aaccagctat ccaaaagtgg ttgaatcgat
    15541 tctttcgaac gatcacttgc tcactttcta tgatttcccc ttggccatac gcaagagtat
    15601 ttattctacg aacttgattg aatcctttaa taaacaaatc aagaaataca gccaccgcaa
    15661 ggaacagttc caaaatgaag agtcgatgga acgtttctta gtctcgtctt ttgatactta
    15721 caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa caggccgaag gcgagctcga
    15781 acaaatgcta agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt
    15841 acacaaaatt attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa
    15901 cttactatat aatcatatta attaaatagt taggagatct gcttatgttt atatctactc
    15961 ttcaaattcg aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa
    16021 atactattat aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct
    16081 tattagatga tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg
    16141 gaattcaaaa tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca
    16201 gcgaagaaga atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa
    16261 caatcttaga agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta
    16321 ttagacctaa caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc
    16381 atcaatttaa cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat
    16441 ctaaatcaaa tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca
    16501 atgacaatat agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg
    16561 acatgtcaga tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc
    16621 ttagagaaat gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa
    16681 aaatatcatc agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta
    16741 ttaccagtgt tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca
    16801 taattggttc agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg
    16861 gttctttagc aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag
    16921 ggttaggaca tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc
    16981 gttcaagaga actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc
    17041 atattcaaaa aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca
    17101 tgaccacaca ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa
    17161 aatcgtttaa tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta
    17221 aagaaaagtt aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg
    17281 ttaaaagaaa tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag
    17341 aaatacttat tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa
    17401 taggtttgat taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg
    17461 atagaataca gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag
    17521 aacatcgatt gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat
    17581 cctccctttt taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa
    17641 ttgagttttt agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg
    17701 acgcattcaa agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat
    17761 atttagaaag aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg
    17821 caattgagtt aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg
    17881 ctattgcttt tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc
    17941 atacaatcga cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa
    18001 caagtgatac agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag
    18061 atgaggatgt tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg
    18121 aggtttcaaa atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa
    18181 agatgttgtg gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac
    18241 aagggtactt acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag
    18301 aataattgct atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt
    18361 acttagtata gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga
    18421 atttattatt tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat
    18481 tgatgattat actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct
    18541 caaaacgtat gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga
    18601 agttcagtat actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat
    18661 tgattttgat atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc
    18721 acgtaatatt gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga
    18781 attacaatac aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt
    18841 tgttggagat attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga
    18901 agaactagaa atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag
    18961 gtctaatcag agattagtag acttctactc taattttcag agttgtcctt acgagattga
    19021 aagcagaggg aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa
    19081 ggaggattta cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca
    19141 tgaaaaagat atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta
    19201 tttaaagaaa gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt
    19261 aaataatcat agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa
    19321 ggttcgaaga agaaagctaa ttgcaaacga tattttaat
//
LOCUS       DAYYJC010000048        19355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000048 DAYYJC010000000
VERSION     DAYYJC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.048"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..2728
                     /locus_tag="ACHA9N_001620"
     CDS             257..2728
                     /locus_tag="ACHA9N_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9085083.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2831..4567)
                     /locus_tag="ACHA9N_001621"
     CDS             complement(2831..4567)
                     /locus_tag="ACHA9N_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085084.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4564..5268)
                     /locus_tag="ACHA9N_001622"
     CDS             complement(4564..5268)
                     /locus_tag="ACHA9N_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9085085.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5399..5902
                     /locus_tag="ACHA9N_001623"
     CDS             5399..5902
                     /locus_tag="ACHA9N_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9085086.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5862..6200
                     /locus_tag="ACHA9N_001624"
     CDS             5862..6200
                     /locus_tag="ACHA9N_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9085087.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6221..7639
                     /gene="ffh"
                     /locus_tag="ACHA9N_001625"
     CDS             6221..7639
                     /gene="ffh"
                     /locus_tag="ACHA9N_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9085088.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7751..8329
                     /locus_tag="ACHA9N_001626"
     CDS             7751..8329
                     /locus_tag="ACHA9N_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9085089.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8333..8854
                     /locus_tag="ACHA9N_001627"
     CDS             8333..8854
                     /locus_tag="ACHA9N_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9085090.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8933..9487)
                     /locus_tag="ACHA9N_001628"
     CDS             complement(8933..9487)
                     /locus_tag="ACHA9N_001628"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9085091.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9740..10015
                     /gene="rpsP"
                     /locus_tag="ACHA9N_001629"
     CDS             9740..10015
                     /gene="rpsP"
                     /locus_tag="ACHA9N_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9085092.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10027..10278
                     /locus_tag="ACHA9N_001630"
     CDS             10027..10278
                     /locus_tag="ACHA9N_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9085093.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10403..11194
                     /locus_tag="ACHA9N_001631"
     CDS             10403..11194
                     /locus_tag="ACHA9N_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085094.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11364..11885
                     /gene="rimM"
                     /locus_tag="ACHA9N_001632"
     CDS             11364..11885
                     /gene="rimM"
                     /locus_tag="ACHA9N_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9085095.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11875..12636
                     /gene="trmD"
                     /locus_tag="ACHA9N_001633"
     CDS             11875..12636
                     /gene="trmD"
                     /locus_tag="ACHA9N_001633"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9085096.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12772..13119
                     /gene="rplS"
                     /locus_tag="ACHA9N_001634"
     CDS             12772..13119
                     /gene="rplS"
                     /locus_tag="ACHA9N_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9085097.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13251..14387)
                     /locus_tag="ACHA9N_001635"
     CDS             complement(13251..14387)
                     /locus_tag="ACHA9N_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085098.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14503..15417)
                     /locus_tag="ACHA9N_001636"
     CDS             complement(14503..15417)
                     /locus_tag="ACHA9N_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9085099.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15508..15936)
                     /locus_tag="ACHA9N_001637"
     CDS             complement(15508..15936)
                     /locus_tag="ACHA9N_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9085100.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15941..16315)
                     /locus_tag="ACHA9N_001638"
     CDS             complement(15941..16315)
                     /locus_tag="ACHA9N_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9085101.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16601..16786
                     /locus_tag="ACHA9N_001639"
     CDS             16601..16786
                     /locus_tag="ACHA9N_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085102.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16798..17046
                     /locus_tag="ACHA9N_001640"
     CDS             16798..17046
                     /locus_tag="ACHA9N_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085103.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17077..17256
                     /locus_tag="ACHA9N_001641"
     CDS             17077..17256
                     /locus_tag="ACHA9N_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085104.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17299..17769)
                     /locus_tag="ACHA9N_001642"
     CDS             complement(17299..17769)
                     /locus_tag="ACHA9N_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085105.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17856..18554
                     /locus_tag="ACHA9N_001643"
     CDS             17856..18554
                     /locus_tag="ACHA9N_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9085106.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18732..19073
                     /locus_tag="ACHA9N_001644"
     CDS             18732..19073
                     /locus_tag="ACHA9N_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085107.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19066..>19355
                     /locus_tag="ACHA9N_001645"
     CDS             19066..>19355
                     /locus_tag="ACHA9N_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9085108.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6374 a         3380 c         3811 g         5790 t
ORIGIN      
        1 taggatgacg ttttggtcga ctaaatttta ccaaaagtga gtctcttttt gagttgaaac
       61 gaagaaaaag tgttagtaga aaatcgcctt cgattttcca ccaacacaaa atattataga
      121 accctttttt tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac
      181 actttccata tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat
      241 caatcgaggt agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac
      301 acaaagatca tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc
      361 agatcaagtc aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt
      421 caaaacaaat cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct
      481 tatcttagcc attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt
      541 tatcatagcc aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga
      601 cgaactaaat gttttaaata cagcaaaagt caatgtcata cgagacggga tagaacaaac
      661 aatagatata gaagaccttg taatagatga cattgttatt ttagagactg gatatcaaat
      721 tccagcagat gcagaggtcg tggaagggaa actgcaagtt aatgaggctt tattgacagg
      781 cgaagcagat gaaataacca aagaaatagg tgatccatta atgtccggca gtttcattgt
      841 ttccggaaaa gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct
      901 gacagtcaaa gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa
      961 tcaattgatc aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca
     1021 aagttacttt tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt
     1081 gatcggtatg atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc
     1141 gactaaacta gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc
     1201 aagggtaaat gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt
     1261 tcaaaaaatg atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc
     1321 atgcattgct gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa
     1381 agcatatttt acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt
     1441 ccaaaaattc agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat
     1501 ggttttgcgc gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg
     1561 ctatcgcgtg ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga
     1621 agcagtcaca ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc
     1681 cacatttgat tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc
     1741 gctcaccgta tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga
     1801 tgcatctaaa ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg
     1861 acgcgtcaag ccagaacaaa agaaaatctt cgttcaattg ataaagcgta acaatacagt
     1921 agctatgaca ggtgatggcg tgaatgatat tctagccatg aaagaagcag actgcagtat
     1981 agcgatggct tccggaaatg aagccgccat gcaggcctca caagtggtct tacttgattc
     2041 tgacttttca cgcatgccag aagttgttgc agaaggacgg cgcgttgtga acaatattga
     2101 gcgatcagcc agcttgtttt tagtgaagaa tattttctcc tttttgctct cactgttttc
     2161 agtgatcttt gctctgacct atcctttaga accatcacaa atcacattga ttagtttgtt
     2221 tactatcgga cttccatctt tcttgcttgc tctagaagaa aacaaaaagc gaatcagagg
     2281 gaaattcatc atgaatgtga tggaaaaagc tgtgccaggt ggactaacag atatgatcgt
     2341 agtcggtgcg ttggtgatct gcggggtgac tttgaatcta aataaaacag atgtttctac
     2401 agcatctacc atgcttttga ttgcagtagg gttcctcgtt ctctataaaa tctgttcacc
     2461 gctgaataaa ttcagaagtc agatcattct tttctgtgct agcggtaact ttttttcggt
     2521 catctttttg cataagctgt tttcaatcac cggtatctca gctgtatcac ttttgctgtt
     2581 gagcattcta tttttctcgg cagattcgat ttttaggcat ttgaccacat tggtagaata
     2641 tttgtttgaa cgtaaaaatg aaaaagagca aaagccgttt agtcgcgtag ctaaaaaatt
     2701 tctatcgata tttaaacgag aaaattgacg aaaagtatct caaaaaagag taaaaaagaa
     2761 caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac gcacgagcag
     2821 ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt ccaagatgac
     2881 tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc gctaacccta
     2941 atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa cgctcaaaga
     3001 tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa aacgatagat
     3061 catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat tggacagcat
     3121 tttcaattag atttttgata attggataaa ggatatctgt tttagtagta aatggttcgt
     3181 tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga taattttgaa
     3241 cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa ttctcgccat
     3301 cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca tctttttgca
     3361 tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc agtaaagtct
     3421 cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg ttgccaacga
     3481 aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc acccctagaa
     3541 tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat gtctgccgac
     3601 cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact tgatgaatca
     3661 gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt tgatagctta
     3721 ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa tcagcatcat
     3781 caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc tcttctgtgg
     3841 tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc gtctgataca
     3901 attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga ttttgcacca
     3961 ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag atcagataat
     4021 agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc caacggcgaa
     4081 aactctcagt tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa atggcttcta
     4141 actggccact ttttttgact ggtaatgcga catataaaag atcctcattc aaagtagtac
     4201 ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt tcgggacggg
     4261 agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt ccagtagcat
     4321 ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca gttttgtttt
     4381 gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct ttttttgtca
     4441 ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc aattgccagc
     4501 caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa atttttttcc
     4561 atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca ccaagtactt
     4621 tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta catctacgat
     4681 tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt ctcggtcgat
     4741 cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg gtgttagttc
     4801 aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga tttgtccaat
     4861 cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa ttcgtctgaa
     4921 tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca aatagtcgtc
     4981 agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg tcagcatcaa
     5041 aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc catccatcgt
     5101 tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta attctaatcc
     5161 ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat tgaacgtcaa
     5221 gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc aaatctcctt
     5281 taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg ctattttaca
     5341 aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag agcagtcaat
     5401 gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac agaaaaaaga
     5461 agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct gtccagaagt
     5521 gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa atggattcga
     5581 tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa cggaagcgta
     5641 caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg atctagtcat
     5701 catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa gtttttccgg
     5761 aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa aaatgactgt
     5821 gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc gaaaagacca
     5881 atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa caaatgaact
     5941 acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa gaatacgaag
     6001 taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg gaggattatg
     6061 aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg gaaaatatga
     6121 ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat gtacagcaaa
     6181 ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg aaagcttaac
     6241 tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat cagaagctga
     6301 cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg ttaacttgca
     6361 agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag aagtgctgga
     6421 aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga cgatcacact
     6481 tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta tcatgatggc
     6541 tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt atttaaagaa
     6601 aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag ctgcgatcga
     6661 ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg gaactgaagt
     6721 cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga aaaacgacta
     6781 tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg atgagttgaa
     6841 acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg ccatgactgg
     6901 tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta caggggtcgt
     6961 catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc gctcagtgac
     7021 aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag aagtattcca
     7081 cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat tgattgaaaa
     7141 agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa tgcgggaaaa
     7201 ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta tgggcccact
     7261 ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg aaaacgtcaa
     7321 ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga cgccagcaga
     7381 aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg ccggttctgg
     7441 aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa gaaaaatgat
     7501 gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt ttggttcagg
     7561 cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa ataagaagaa
     7621 aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa aatgtctact
     7681 gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc taaacgaaga
     7741 ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc aatatcatga
     7801 tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat tgatgcttga
     7861 tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag cattcgatga
     7921 agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa aaatcagtga
     7981 actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag ccgtccaaaa
     8041 cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt atctttggtc
     8101 gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag tcccaacaac
     8161 aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta aatttatcgg
     8221 aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc accttgacag
     8281 ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa gcatgcgaaa
     8341 aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa cggatgatct
     8401 tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt tttatgaaac
     8461 aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat atttggagac
     8521 cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac aattacaatt
     8581 gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc tacaaatgac
     8641 agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc tattggaaca
     8701 tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg acgggattcc
     8761 tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga cacgattcgg
     8821 tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa aaaggcaact
     8881 atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct ttttactctg
     8941 ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa cttaatgttc
     9001 cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata tatgcttccg
     9061 gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg tggtttgcgc
     9121 caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta tgatcccaaa
     9181 cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact gctggcacag
     9241 aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact tcctcacgaa
     9301 aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg taaaatggca
     9361 gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc gcaaaagctt
     9421 ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat ccaatttttt
     9481 ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa gataagtaac
     9541 tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta cttattttta
     9601 acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa tcaagctgta
     9661 aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt gaataaaaca
     9721 atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg gttctaaaaa
     9781 gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg gacgtttcat
     9841 cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt taaaagaaga
     9901 cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac gtaacatcct
     9961 ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga aataaggtga
    10021 ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct agtcacttat
    10081 cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta caatctaaca
    10141 gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc taaagcaatc
    10201 cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt gaatatcatc
    10261 gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca gcttttttgt
    10321 tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc gtatccttat
    10381 aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat ttgctctatt
    10441 gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat caagcagtat
    10501 atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca ccactgaaca
    10561 aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga aagaacaaca
    10621 gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca atatcgcagc
    10681 gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc ccctcgggtt
    10741 gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc aatatgggtt
    10801 tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca caaatggtca
    10861 gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag gttcaagttt
    10921 tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata tagaaggcca
    10981 agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca gtctaccagc
    11041 acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc gagccgtaga
    11101 agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc ctatgtctgc
    11161 gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc tgagcccatc
    11221 atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt gcataatttt
    11281 ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa ataaaacaga
    11341 caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa atcgtaaaca
    11401 cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca gaagaacgtt
    11461 acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg gaattaacag
    11521 tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga catccttcta
    11581 tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac aactctgtag
    11641 atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta ttagaagatg
    11701 gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat gtttgggtcg
    11761 ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc gtcaaagaag
    11821 tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt gacgatgaag
    11881 attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga atccatcatc
    11941 ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg cgattattca
    12001 gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg tatgctgctc
    12061 aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc ggatgtcaaa
    12121 aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat ggcagaagaa
    12181 ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata tgatgaacgt
    12241 atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac tgggggagaa
    12301 ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt gttagggaat
    12361 cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc ccaatatact
    12421 cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg aaatcataaa
    12481 ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg tcggcctgat
    12541 cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga aatcaagaaa
    12601 gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc ttctttacag
    12661 taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact attacgatat
    12721 tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac catgaatcca
    12781 ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt ccgccctggt
    12841 gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat ccagttattt
    12901 gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac agtacgtaaa
    12961 gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg tgttgcaaaa
    13021 atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct acgtgcatta
    13081 cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc agactgagaa
    13141 agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat taacgatttc
    13201 tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag ttacagtttt
    13261 tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata aatcttttca
    13321 gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg agttcctagt
    13381 tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt tttatcaatc
    13441 ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg gaaaggagtt
    13501 cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag ctcattgtaa
    13561 atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt ttttgtagaa
    13621 tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc atttataact
    13681 actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat tgcttctcca
    13741 ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt attgtgtgaa
    13801 gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc tttaattttt
    13861 atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt tttttctaca
    13921 tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat tacactgacg
    13981 tacttatttg acaagtcatc tttatatatc atatcttcaa aaatattatt taaatctact
    14041 gtattaatac cagaaacgat gtaagtctca ggtatctttt tctttatagt gtttaagata
    14101 ttattcgtag gataatatgt agactcctta acttgtcttt tgtaaataac taaccattct
    14161 tcgacaagct catgaaatgt tagatcaggc ttctgaagtg ttttttcgtt taattttata
    14221 tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga tttattttta
    14281 taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg ctcattatat
    14341 ttatatactt tttttccgtt tttatcagtt ttggtttcaa tccacattgt tttcatctcc
    14401 tatttgttgc tataatagga atggataagt aagccaatta tagcaggttt atttttcatc
    14461 acgtccacaa actttggcga gggagggggc gtgttttttt gtttagtagc tacctttcca
    14521 tgaagtatct gcgaagaaca cttcaaatgt tgttccatca tttttcactt caaaatatgc
    14581 tgttccttgt gcggatttac ccggttggat ggtttccgaa aagtaatctc tactagctaa
    14641 atcagattta atatctgatg aatcataaaa ttctaaataa cttgaacttg catcaaaagg
    14701 tgtagtccct gtgttttcta caagaaaatc tacctttgcg taaaataggc cttctggttt
    14761 atagaaatca tcaccgtaat cttttgatac agactggaca gttacgttaa tagagttacc
    14821 attttcagaa gtaaaaggaa ctgcttctcc tatactatgt gcaactgatg ccgtctgggg
    14881 ttctgaagac gaagttgtag ttgtagttgt agtagagctt gaatctttgg attttccatc
    14941 tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg gactaaccca
    15001 aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca tctctaattt
    15061 ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt tataataatc
    15121 tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag cattgtctaa
    15181 ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg tctgataaac
    15241 ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta gtttttttgt
    15301 ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat taggactagt
    15361 ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt ttttcatttt
    15421 taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag gtttttagtc
    15481 cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc tttctggaag
    15541 tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt cttctttgta
    15601 tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt tgcttctaga
    15661 tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcgt gacctaattc
    15721 gtgagcgcaa atgtagaatc tttcctcaga gtctctcagt tcatcattta agaagattat
    15781 tgcacgacct ctaatttctt gaaactgtcc tttgggattc tcgataaaag gaacgtattg
    15841 aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt atttccgctt
    15901 caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt ttgccttttt
    15961 ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt acgcgttgga
    16021 cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa tctagtaatt
    16081 tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt ctaccgagta
    16141 aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta gtactaggag
    16201 ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct tccaattgag
    16261 ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa ttcattttcg
    16321 cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg acaactaata
    16381 aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact tgataactaa
    16441 taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc tgtgtcttat
    16501 ttagctatgt ctatatacta ataaaaatat tagttattgt caacgaatat tagctaattt
    16561 tttagcttac agattatttt tttagaaagg agctattttt atgtctgaga atttagactt
    16621 aaaaattcga gcggagatga gaaaaagaag aatgactttc aaagaactag ctgcgcttat
    16681 cggtatttca ggagcttatt tatcagatat tctaaacggc aatcgtgatg gaaagaaagc
    16741 acaacagcat atcgaaacag tgaaaaaaat attggacatc cgataggggg gtaggcgatg
    16801 acaaaactaa aaaaacaaga ttttgtaaaa aaatacaatt attctccatc tacttatcaa
    16861 cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca
    16921 ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat
    16981 aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat
    17041 ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcattg
    17101 gtattcagcg caccgctaat catctacgct ttaggacttt ggggaagcag gcaagcgttg
    17161 ataggaacga ttgtttacat ggtttggatt tttatggggc ttgatgaagc tgaagctgag
    17221 tacagagcga aaaagccaac cgagggggct gactaaaaat gaatagaaaa gagaaactag
    17281 aatgtatatt actattactc agtttaattc tttcactaat ttctctattg ggaagtttgt
    17341 atttttgata tcaacaattt tgtggatttg ggttctattt agagtaaata tcatttttgt
    17401 ttggtgatta aggagtttat taaaattaat tttccctact tcaaatgcaa ggtaacaaga
    17461 tatagcactt ttaggaggaa tatttagcgg tacagcttca gagtatattc tattatcatc
    17521 gactgatcct cctttagcaa ttaatttctt aaatggagta gctgaccatc tgcggtttaa
    17581 gttagcggat gaaagttcta aattaactaa catagctggt tctgttgaat agttagaaat
    17641 gattactttt gtataaatca tatctgcagc gaagtaagaa gcattaagtt caacttctag
    17701 ttgagggcgc attcctttta tttttatccc tgttaaaaac gtactcaata taaaacttat
    17761 tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc aaaataaaca ccaccagttt
    17821 ttaactaaat tataccaaaa aggagagaag aaataatgca agaattagta attttgaaaa
    17881 ataaagaagc tgtgactacg agcttacaag tggcagaaag cttcgagaaa aaacatcaac
    17941 atgttttaag agatattgat gcactaaaaa aagatgtgtc caattttgga cagatgtttg
    18001 tagaaggtaa tgaaccagat tcatatggca gaaatcgacg agttttcttc attagtagag
    18061 atggattttt cttgctggct atggggttta caggaaagaa agctatctac ttcaaacaaa
    18121 aatacattga agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa
    18181 caattgaaga tatgatgatc tatcaattag aagaaatgaa agatgttaaa aaagatgttt
    18241 ccatgcttaa agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag
    18301 gaaatatgaa agttatggga gttctagggg gaaaagaaag ccgagcttat gaagaaatca
    18361 gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg taccttttca atcccaagat
    18421 atggcgagtt acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc
    18481 cagaaactgc gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg
    18541 atgacaatga atagagctga agcgctaaga atagggacgg caattgctaa ttgctggtgg
    18601 aaatactata aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa
    18661 caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga
    18721 gattttagca tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt
    18781 ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga
    18841 atgaagaact agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg
    18901 tttatcttat ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag
    18961 atggcttgat tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt
    19021 acggaccagg taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa
    19081 gggacaacga actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa
    19141 ggaaattttg attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca
    19201 caggcaacgg taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa
    19261 gactttcttg ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct
    19321 gtctcagtaa cggttaaagg acacacggaa accga
//
LOCUS       DAYYJC010000049        19199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000049 DAYYJC010000000
VERSION     DAYYJC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.049"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..2542
                     /locus_tag="ACHA9N_001646"
     CDS             101..2542
                     /locus_tag="ACHA9N_001646"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9085109.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="ACHA9N_001647"
     CDS             complement(2872..3576)
                     /locus_tag="ACHA9N_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085110.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="ACHA9N_001648"
     CDS             complement(3763..4008)
                     /locus_tag="ACHA9N_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085111.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="ACHA9N_001649"
     CDS             complement(4025..4876)
                     /locus_tag="ACHA9N_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9085112.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="ACHA9N_001650"
     CDS             5042..6046
                     /locus_tag="ACHA9N_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9085113.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="ACHA9N_001651"
     CDS             complement(6287..6643)
                     /locus_tag="ACHA9N_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9085114.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="ACHA9N_001652"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="ACHA9N_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9085115.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="ACHA9N_001653"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="ACHA9N_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9085116.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11764)
                     /locus_tag="ACHA9N_001654"
     CDS             complement(11003..11764)
                     /locus_tag="ACHA9N_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085117.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11931..12563
                     /locus_tag="ACHA9N_001655"
                     /pseudo
     CDS             11931..12563
                     /locus_tag="ACHA9N_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            12622..13482
                     /locus_tag="ACHA9N_001656"
     CDS             12622..13482
                     /locus_tag="ACHA9N_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9085118.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13667..15982
                     /locus_tag="ACHA9N_001657"
     CDS             13667..15982
                     /locus_tag="ACHA9N_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9085119.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /gene="dnaG"
                     /locus_tag="ACHA9N_001658"
     CDS             16164..18005
                     /gene="dnaG"
                     /locus_tag="ACHA9N_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9085120.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="ACHA9N_001659"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="ACHA9N_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9085121.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5553 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaa
//
LOCUS       DAYYJC010000050        19130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000050 DAYYJC010000000
VERSION     DAYYJC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.050"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..703)
                     /locus_tag="ACHA9N_001660"
     CDS             complement(<1..703)
                     /locus_tag="ACHA9N_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9085122.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="ACHA9N_001661"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="ACHA9N_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="ACHA9N_001662"
     CDS             1758..2837
                     /locus_tag="ACHA9N_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9085123.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2834..3462
                     /locus_tag="ACHA9N_001663"
                     /pseudo
     CDS             2834..3462
                     /locus_tag="ACHA9N_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(3572..3799)
                     /locus_tag="ACHA9N_001664"
     CDS             complement(3572..3799)
                     /locus_tag="ACHA9N_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9085124.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="ACHA9N_001665"
     CDS             complement(3902..6490)
                     /locus_tag="ACHA9N_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9085125.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="ACHA9N_001666"
     CDS             complement(6510..7169)
                     /locus_tag="ACHA9N_001666"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9085126.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA9N_001667"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="ACHA9N_001667"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9085127.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="ACHA9N_001668"
     CDS             complement(7942..8808)
                     /locus_tag="ACHA9N_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9085128.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="ACHA9N_001669"
     CDS             8917..9558
                     /locus_tag="ACHA9N_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9085129.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA9N_001670"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="ACHA9N_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9085130.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="ACHA9N_001671"
     CDS             complement(10999..11898)
                     /locus_tag="ACHA9N_001671"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9085131.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="ACHA9N_001672"
     CDS             complement(11900..12697)
                     /locus_tag="ACHA9N_001672"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9085132.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="ACHA9N_001673"
     CDS             complement(12675..13379)
                     /locus_tag="ACHA9N_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9085133.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="ACHA9N_001674"
     CDS             13510..14085
                     /locus_tag="ACHA9N_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9085134.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="ACHA9N_001675"
     CDS             14203..14880
                     /locus_tag="ACHA9N_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9085135.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA9N_001676"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="ACHA9N_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9085136.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="ACHA9N_001677"
     CDS             complement(16943..17941)
                     /locus_tag="ACHA9N_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9085137.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="ACHA9N_001678"
     CDS             complement(18167..18820)
                     /locus_tag="ACHA9N_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9085138.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DAYYJC010000051        18972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000051 DAYYJC010000000
VERSION     DAYYJC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.051"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACHA9N_001679"
     CDS             complement(17..538)
                     /locus_tag="ACHA9N_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9085139.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACHA9N_001680"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACHA9N_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9085140.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHA9N_001681"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACHA9N_001681"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9085141.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACHA9N_001682"
     CDS             5502..6671
                     /locus_tag="ACHA9N_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9085142.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /gene="glmM"
                     /locus_tag="ACHA9N_001683"
     CDS             6695..8050
                     /gene="glmM"
                     /locus_tag="ACHA9N_001683"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9085143.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHA9N_001684"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="ACHA9N_001684"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9085144.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="ACHA9N_001685"
     CDS             10336..10626
                     /locus_tag="ACHA9N_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085145.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="ACHA9N_001686"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="ACHA9N_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9085146.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="ACHA9N_001687"
     CDS             11667..12326
                     /locus_tag="ACHA9N_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9085147.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="ACHA9N_001688"
     CDS             12483..13415
                     /locus_tag="ACHA9N_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085148.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /gene="tig"
                     /locus_tag="ACHA9N_001689"
     CDS             13569..14858
                     /gene="tig"
                     /locus_tag="ACHA9N_001689"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9085149.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="ACHA9N_001690"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="ACHA9N_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9085150.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /gene="yihA"
                     /locus_tag="ACHA9N_001691"
     CDS             16438..17025
                     /gene="yihA"
                     /locus_tag="ACHA9N_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9085151.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="ACHA9N_001692"
     CDS             complement(17099..17254)
                     /locus_tag="ACHA9N_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9085152.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18718
                     /locus_tag="ACHA9N_001693"
     CDS             17390..18718
                     /locus_tag="ACHA9N_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085153.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18729..18938
                     /locus_tag="ACHA9N_001694"
     CDS             18729..18938
                     /locus_tag="ACHA9N_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085154.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6218 a         3377 c         4082 g         5295 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    18901 atatgaaaaa aacaagaagc gaaaataaaa aacgataaaa cggcttgatg atagacaaaa
    18961 aaataaaaaa cc
//
LOCUS       DAYYJC010000052        18735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000052 DAYYJC010000000
VERSION     DAYYJC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.052"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..526
                     /locus_tag="ACHA9N_001695"
     CDS             251..526
                     /locus_tag="ACHA9N_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085155.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="ACHA9N_001696"
     CDS             539..2611
                     /locus_tag="ACHA9N_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9085156.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2779..4662
                     /locus_tag="ACHA9N_001697"
     CDS             2779..4662
                     /locus_tag="ACHA9N_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9085157.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4674..5621
                     /locus_tag="ACHA9N_001698"
     CDS             4674..5621
                     /locus_tag="ACHA9N_001698"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9085158.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="ACHA9N_001699"
     CDS             5641..6168
                     /locus_tag="ACHA9N_001699"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9085159.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="ACHA9N_001700"
     CDS             complement(6231..6884)
                     /locus_tag="ACHA9N_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9085160.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="ACHA9N_001701"
     CDS             7016..7858
                     /locus_tag="ACHA9N_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9085161.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="ACHA9N_001702"
     CDS             8016..8912
                     /locus_tag="ACHA9N_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9085162.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="ACHA9N_001703"
     CDS             8915..9628
                     /locus_tag="ACHA9N_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9085163.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="ACHA9N_001704"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="ACHA9N_001704"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9085164.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="ACHA9N_001705"
     CDS             10163..10390
                     /locus_tag="ACHA9N_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9085165.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="ACHA9N_001706"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="ACHA9N_001706"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9085166.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11718)
                     /locus_tag="ACHA9N_001707"
     CDS             complement(11176..11718)
                     /locus_tag="ACHA9N_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9085167.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="ACHA9N_001708"
     CDS             complement(11955..12353)
                     /locus_tag="ACHA9N_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9085168.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12613..13473
                     /gene="ylqF"
                     /locus_tag="ACHA9N_001709"
     CDS             12613..13473
                     /gene="ylqF"
                     /locus_tag="ACHA9N_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9085169.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13466..14233
                     /locus_tag="ACHA9N_001710"
     CDS             13466..14233
                     /locus_tag="ACHA9N_001710"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9085170.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14289..15146
                     /gene="dprA"
                     /locus_tag="ACHA9N_001711"
     CDS             14289..15146
                     /gene="dprA"
                     /locus_tag="ACHA9N_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9085171.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15268..17346
                     /gene="topA"
                     /locus_tag="ACHA9N_001712"
     CDS             15268..17346
                     /gene="topA"
                     /locus_tag="ACHA9N_001712"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9085172.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17315..18691
                     /gene="trmFO"
                     /locus_tag="ACHA9N_001713"
     CDS             17315..18691
                     /gene="trmFO"
                     /locus_tag="ACHA9N_001713"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9085173.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3898 g         5088 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta
    12421 gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag
    12481 aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt
    12541 gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga
    12601 atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa
    12661 gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct
    12721 ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta
    12781 ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt
    12841 aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta
    12901 gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc
    12961 aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg
    13021 atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa
    13081 ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta
    13141 tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa
    13201 gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat
    13261 tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca
    13321 ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc
    13381 agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt
    13441 tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt
    13501 gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa
    13561 aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa
    13621 ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg
    13681 gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc
    13741 agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga
    13801 gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg
    13861 gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat
    13921 gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac
    13981 cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat
    14041 tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt
    14101 gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga
    14161 aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa
    14221 atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg
    14281 gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca
    14341 gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg
    14401 gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa
    14461 gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt
    14521 gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt
    14581 tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac
    14641 acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg
    14701 gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa
    14761 tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg
    14821 ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc
    14881 ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt
    14941 tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac
    15001 agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac
    15061 attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag
    15121 atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg
    15181 ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca
    15241 cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca
    15301 cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt
    15361 gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat
    15421 gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca
    15481 gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt
    15541 gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc
    15601 aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta
    15661 gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc
    15721 agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc
    15781 gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat
    15841 tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga
    15901 atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga
    15961 attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct
    16021 gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga
    16081 acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa
    16141 ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca
    16201 aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat
    16261 caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg
    16321 agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt
    16381 tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca
    16441 atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa
    16501 ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa
    16561 aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa
    16621 caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa
    16681 gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc
    16741 tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat
    16801 tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa
    16861 ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc
    16921 tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc
    16981 aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta
    17041 gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca
    17101 atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga
    17161 aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact
    17221 tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag
    17281 aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa
    17341 aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc
    17401 cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat
    17461 gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc
    17521 caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg
    17581 ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc
    17641 cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc
    17701 attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc
    17761 aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga
    17821 tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga
    17881 taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat
    17941 gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact
    18001 taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca
    18061 gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa
    18121 aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt
    18181 atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg
    18241 gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag
    18301 ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct
    18361 gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact
    18421 acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca
    18481 agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca
    18541 accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa
    18601 agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga
    18661 attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat
    18721 agtctcgctc ttttt
//
LOCUS       DAYYJC010000053        18368 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000053 DAYYJC010000000
VERSION     DAYYJC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.053"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACHA9N_001714"
     CDS             complement(111..566)
                     /locus_tag="ACHA9N_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085174.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACHA9N_001715"
     CDS             complement(720..1928)
                     /locus_tag="ACHA9N_001715"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9085175.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACHA9N_001716"
     CDS             complement(2040..2372)
                     /locus_tag="ACHA9N_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9085176.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACHA9N_001717"
     CDS             2520..3395
                     /locus_tag="ACHA9N_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9085177.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACHA9N_001718"
     CDS             complement(3477..4058)
                     /locus_tag="ACHA9N_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9085178.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACHA9N_001719"
     CDS             complement(4064..5323)
                     /locus_tag="ACHA9N_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9085179.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACHA9N_001720"
     CDS             complement(5325..6371)
                     /locus_tag="ACHA9N_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085180.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACHA9N_001721"
     CDS             complement(6606..6881)
                     /locus_tag="ACHA9N_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9085181.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHA9N_001722"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACHA9N_001722"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9085182.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHA9N_001723"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACHA9N_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9085183.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHA9N_001724"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACHA9N_001724"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9085184.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACHA9N_001725"
     CDS             complement(10709..11344)
                     /locus_tag="ACHA9N_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9085185.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACHA9N_001726"
     CDS             complement(11410..12813)
                     /locus_tag="ACHA9N_001726"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9085186.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACHA9N_001727"
     CDS             complement(12800..13831)
                     /locus_tag="ACHA9N_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085187.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACHA9N_001728"
     CDS             13862..14083
                     /locus_tag="ACHA9N_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9085188.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACHA9N_001729"
     CDS             complement(14248..14985)
                     /locus_tag="ACHA9N_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9085189.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACHA9N_001730"
     CDS             complement(15138..16478)
                     /locus_tag="ACHA9N_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9085190.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACHA9N_001731"
     CDS             complement(16715..18238)
                     /locus_tag="ACHA9N_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9085191.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5183 a         3914 c         3105 g         6166 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttccc
//
LOCUS       DAYYJC010000054        16583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000054 DAYYJC010000000
VERSION     DAYYJC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.054"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(428..1363)
                     /gene="murB"
                     /locus_tag="ACHA9N_001732"
     CDS             complement(428..1363)
                     /gene="murB"
                     /locus_tag="ACHA9N_001732"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9085192.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1436..2356)
                     /locus_tag="ACHA9N_001733"
     CDS             complement(1436..2356)
                     /locus_tag="ACHA9N_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9085193.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2469..3221
                     /locus_tag="ACHA9N_001734"
     CDS             2469..3221
                     /locus_tag="ACHA9N_001734"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9085194.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            4085..4618
                     /locus_tag="ACHA9N_001735"
     CDS             4085..4618
                     /locus_tag="ACHA9N_001735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9085195.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(5108..5632)
                     /locus_tag="ACHA9N_001736"
     CDS             complement(5108..5632)
                     /locus_tag="ACHA9N_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9085196.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5625..6098)
                     /gene="tsaE"
                     /locus_tag="ACHA9N_001737"
     CDS             complement(5625..6098)
                     /gene="tsaE"
                     /locus_tag="ACHA9N_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9085197.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(6221..7201)
                     /gene="pta"
                     /locus_tag="ACHA9N_001738"
     CDS             complement(6221..7201)
                     /gene="pta"
                     /locus_tag="ACHA9N_001738"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9085198.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7354..8034)
                     /locus_tag="ACHA9N_001739"
     CDS             complement(7354..8034)
                     /locus_tag="ACHA9N_001739"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9085199.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            8160..9029
                     /locus_tag="ACHA9N_001740"
     CDS             8160..9029
                     /locus_tag="ACHA9N_001740"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9085200.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            9055..9570
                     /locus_tag="ACHA9N_001741"
     CDS             9055..9570
                     /locus_tag="ACHA9N_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9085201.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9617..10180)
                     /locus_tag="ACHA9N_001742"
     CDS             complement(9617..10180)
                     /locus_tag="ACHA9N_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085202.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(10235..10414)
                     /locus_tag="ACHA9N_001743"
     CDS             complement(10235..10414)
                     /locus_tag="ACHA9N_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085203.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10491..11000)
                     /locus_tag="ACHA9N_001744"
     CDS             complement(10491..11000)
                     /locus_tag="ACHA9N_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085204.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(11096..11476)
                     /locus_tag="ACHA9N_001745"
     CDS             complement(11096..11476)
                     /locus_tag="ACHA9N_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9085205.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11806..12555
                     /gene="pnuC"
                     /locus_tag="ACHA9N_001746"
     CDS             11806..12555
                     /gene="pnuC"
                     /locus_tag="ACHA9N_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9085206.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12739..14016)
                     /locus_tag="ACHA9N_001747"
     CDS             complement(12739..14016)
                     /locus_tag="ACHA9N_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085207.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(14095..14739)
                     /gene="pcp"
                     /locus_tag="ACHA9N_001748"
     CDS             complement(14095..14739)
                     /gene="pcp"
                     /locus_tag="ACHA9N_001748"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9085208.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14938..15882)
                     /locus_tag="ACHA9N_001749"
     CDS             complement(14938..15882)
                     /locus_tag="ACHA9N_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9085209.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15884..16570)
                     /locus_tag="ACHA9N_001750"
     CDS             complement(15884..16570)
                     /locus_tag="ACHA9N_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9085210.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4776 a         3433 c         2913 g         5461 t
ORIGIN      
        1 taaatttaga caaacctaat gtttcatata acttaggcag cactacataa ttctttttct
       61 caaattctaa atatctagct agcagcagtt tttctctatc ttcctcttct ataaggtaat
      121 ccaacttcat atcctacacc tcaaaaaatg cctcatacca attacttgat atgcagatga
      181 aagttcatct tttcacctat actgatttta tgatggatta tgcttgtttt ttcatcagtt
      241 attctcattt tttatctttt ttttgagaat attgcaaaaa atggaaaaat ttatgaggaa
      301 atattctata ttttttgttt tattgtgtta aaatgtacaa gatttgcgcc atttgcgtaa
      361 acaaccacaa aaaagagcag gacggccacc gtcctgctct tttacttatt ttaagttcca
      421 ttacttatta attggctaaa cttctgcttg tttctgatat tgcttgtttt tctccaatga
      481 tccgcacttc tgtttctaaa gacacatcaa atttttcttt gattacttct tggatgtgcg
      541 caatcaattc aatataatct gtcgctgtcg catgatcgat attgacgatg aatccagcat
      601 gtttttcaga gacttgtgca ccgccccatt tcaggccttg caatcctgct tgttggatca
      661 gctgacctgt atagtgtcct actgggcgct taaagacact tccgcatgaa gggtattcta
      721 atggctgttt tgattgacgc aattcggtca actcatccat tcttgctttg attgcttcat
      781 aatcccctgt ttgcaaactg aatcgagctt ccaatacgat agcacgtaat tcttggactt
      841 tactgtgacg ataagaaaat accatttcgt cttttgtcaa agttttcaat gttccatctt
      901 caagcaaaag atccacttca gcaaaaacat ctttgatctc tccgccatac gcacctgcat
      961 tcatataaac cgctccaccg acacttcccg ggatgccgca agcaaattca aatccagtca
     1021 atgattctgc caaagctgca taagtcgtat caatcagtga ggcacctgct tccgcaatga
     1081 ccatcgttcc tttgacatga atctgtttca tttcagtcag catgatgact acaccgcgaa
     1141 tcccgccatc ttgaacgatc agattgcttg catttcctaa taccatccaa ggtatatcat
     1201 ggattcggca ataatcgatg atttgcttta cttcttcttg ttttttcggg aatgcaagga
     1261 catcagctgg tccacctgtt ttcgtaaacg tatagttcat caatggttca tcaaacaata
     1321 tagtaatcgt ggctaagctt tcaactattt cttctttatt cactgaggat tttcctttct
     1381 tttctcaaac gtcctcatta ttctatcacg cttaatacaa tcttgccagt cttgttcaaa
     1441 gttgatgacg gtagtgatct ttaagcactt cctcacataa ttcatgactt cttacaactt
     1501 ctttttgttt aagcaattct gtttccgatt tacctaaacg gatctgagaa aagaaggcct
     1561 ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt ccagtcgcca aattctttca
     1621 ctttttttcc atcagcctct tgaatgacaa gctgggtcag atcgtttact tcataagtac
     1681 catttttact tgttacttga tatttttcta tattagcgcc acttgctaaa tccatcgtta
     1741 aaatagccgt ttgattttgt gtttctaatt gtaaaatggc atattccaaa aattcctttg
     1801 tttctcttat ctggtatttt gttctcaaaa tgggttcatc taataaatac acagctgtat
     1861 caataagatg taaaaacaaa tcataaagga cgaactctgt cgtttctttt gccgctattc
     1921 gatttttttg caggtggatc aaccgcttat ctgggatacg tttcaattct tctaccatag
     1981 gagcaaagcg ccggttgaaa ccgatcatca aaattatttg gttcttatca gctaattctt
     2041 gcaattcacg aacttcattt agatggacac tcagtggttt gtcaatataa acatggatac
     2101 cattttctag acattttttg gctaattcat aatgtacttt tgttgctgcg tggatcatac
     2161 atgcttcgat tttttcttct aaaagctcat cgatcgtttc atatagatgt tggaacccgt
     2221 actctttttg aattctttct tttgtttctt tatttcttgt tgcaaaataa aaatcaagtg
     2281 ttccttgttt tttcgcataa gttggtaaat atgctttttc tgcgatattc cctacaccga
     2341 ttacaccaat cttcatctac tttcctcctg gatcatttag ttactttcat cttacaccat
     2401 tttttactaa aatgaggtac aatagtatcg caaaggtaag gaaattaatt cgaaggagtg
     2461 tgcttttatt gaaatttatc tcttggaacg taaacgggct tcgtgccatc gtaaataaaa
     2521 attttttaga agtttttcat gagttagacg ccgatttttt ctgtctgcag gaaacgaaac
     2581 tccaagcagg acaaattgat ttagatcttc caggttatta tcaatattgg aattacgcgg
     2641 aacgaaaagg ctattccggc acagcgattt ttgctaaaaa accagcattg aatgccactt
     2701 acggaatggg tatcgatatc catgatacag aaggccgtct gattactttg gaatattccg
     2761 atttctttct tgtaacctgt tacacgccaa attctcaaag cgaattaaaa cgtttagatt
     2821 accgattaga atgggaagaa gccttttata attatttgga aaatctaaaa aaacaaaaac
     2881 cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa gattgatttg aaaaactgga
     2941 aaacgaatca aaaaaatgct ggattcactc cagaagaaag agcggctctt tctcgactgt
     3001 tggataacgg gtttatcgat acattccgtt atttttatcc aacccaggaa ggtgtctact
     3061 cttggtggaa ctaccgattc aattcaagga aaaacaatgc tggttggcgg atcgattact
     3121 ttttgacgag caaagattta gagccaggac tagctgatgc aaagatccat acggacatta
     3181 tgggcagtga tcattgtcca gtagaattag atttgaaata aagaggttgt gaaaaagtcg
     3241 tttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt tgcataaatt
     3301 tggcataata ccgagaaact ggcttttgaa cactgtttat cgagagattg tgaaaaagct
     3361 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
     3421 ttgggcttat taccgcagat acaagctttt gaacaccgtt tattaagagg ttgtgaaaaa
     3481 gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     3541 tggttgggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtgaa
     3601 aaagctgcac tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc
     3661 gtttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt gagaagttgt
     3721 gaaatcagcg tcctgacctg agtattagag aaacaaatgg caaaatagtt cctcatattt
     3781 tgctattttt tgagctaata ccgagggtca gctgattgaa caccgtttag tggagaagtt
     3841 gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt tgaaaatggt tcctcacaac
     3901 tttgcataaa tttgacttaa taccgagaag tcatgatccc catctaactc caaatcacaa
     3961 ggaatgagtt caaaaactag cttttctcaa caaatattac ataaaaaatt ctatgttttt
     4021 tgttaaattt atttgctttt ttcaaaagta tgttacagta atttcccaag gaggttttac
     4081 agaaatgaat tttgttgcca tggattttga aactgccaat catcagcctt atagtgcttg
     4141 ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt gatgaattct atacattgat
     4201 acagcctgag actccttttt tctggcgaaa tgtccaaatt cacggaatcc atcaagaaga
     4261 cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact atccaaccat attttcaaaa
     4321 aaatcgttta gtcgttgcac ataatgctgc ttttgataca aaggtcttag cgggatgctt
     4381 agattattat catcttgaac agcctaatta tctttcttta tgtacagtga aaagtagccg
     4441 tcgcctgttt cctgaatttc caaatcatcg attgaacact gtgtgcgaaa atttgaacat
     4501 ccaattagat catcaccatg atgcattgga agatagtcgt gcatgtgcgg aaatcctgct
     4561 ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa cttgtgacta taaagtaaaa
     4621 gaatacttat gaaacacaca aagaggtcgt gagcctgccg tttcactccg agtcacaagg
     4681 agcaatttca aaaaatagct tctcatgttt attaaatatt gtgactggtg accgaggagc
     4741 tggcaggaga acaccgttta ttggagaggt tgtgaaaaag ctgttctgca tcaagtaata
     4801 agaacagcca aacaaaaata gcttctcata tttttcattt ggttggactt attaccgcag
     4861 atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa tcagcgtttt gacctgagta
     4921 ttagagaaac aaacggcaaa atagttcctc atattttgcc gttttttgag ctaataccga
     4981 aggtcagctg attgaacacc gtttattcaa aaaaaatgga ctgcgacacg acttttgtca
     5041 cagtcccttc gtgtgttttt ctgccttctc aaaaggatga cagtttctca ctcttttcct
     5101 atttcttcta ttcaatcaaa taactcattt tatatacatc taaaaattca cctagatctg
     5161 ttcttgcccc tctaggcatg accgcttcga tttggaagcc catttttcgg taaaggtgta
     5221 cggctctttc attacgaact tgaacgtcaa gctccaatcg aaaaagtaca cccatttcct
     5281 tcgcccaact gatgatttct tctagcataa gagtgcctag tcccattccc caatattcct
     5341 gcagaatact gataccgact tctcccacgt gagacaaacg aaattgactg tctgccttga
     5401 cagatgctgt tccaataatc gtcccttcat agattgcaag cagcaataaa ttattattgc
     5461 tttcgtatat ataatccaac tcttcttcta aggtttctgg tggtaacagg agttctgctt
     5521 catccaaaac taaaaattct gtctccttat taacttgtcc catcatcaaa agaatgtctt
     5581 ttgcatctcc tggcactgct tcccgcaaga tgatcccaac ttcttcactc atgattttca
     5641 gcccattttt ctagaattcg ctgtttgaag gtttcacttt gctccccgta tgagattagt
     5701 ttcacatacc gcttttctaa atctgtatca cttttttcta aaatcagttc caaataatcc
     5761 tccggcagtg cttcttctaa tagatttccc cattcaacaa cggaaagccc atcaccttca
     5821 aaatattcat ctaaccctaa atctgctccg ctagctgcta cccgataaat atccatatga
     5881 tacaacggaa tccgaccttg actatattct ctaataatcg tgtatgtcgg acttttgatc
     5941 atttgcgtta tccctaatcc ttgggcaatt cctttcgtca acgttgtttt acccgcacct
     6001 agatcaccag tcaatatcag attgtcacct ggcattgcgg aatgaccaat tgcctctgcg
     6061 aattgttctg tcattgttaa tcctgatagt tcgatcattt ttcatcttct cccgctcttc
     6121 tttctttttg ccagtatatc gcgtaatgaa gtttttttaa agaaaaaatg aactagtcaa
     6181 gaacagcagc tgctgttttt ggctagttcg cgtgtatttg ttaatcaatc aatgtttgtg
     6241 ctgctgtgat aatcgataat ttatatacat cttcttcatt tgctccacga gacaaatcag
     6301 aaaccggttt gttcaagcct tgtagaattg gtccgattgc ttcaaagtta ccgaaacgct
     6361 gagcaatttt atagccgata tttcctgact gcaattctgg aaatacaaag actgttgctt
     6421 gaccagctac ttctgaatct ggcgctttca gttgtgcaac tgaaggaaca taagaagcat
     6481 caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag ttttttagcg attttagttg
     6541 cttcaactac tttgtctact tctggcgatt tcgctgagcc ttttgtcgag aagctcatca
     6601 ttgctacttt tggttcgata tcgaacaatt ctgctgtttt tgcagaatct acggcgattt
     6661 cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca atccgaaaag acatattttt
     6721 cttgatctct tccacgtacc atgatcatcg ctccgcttgt ccgactgatg ccaggctttg
     6781 ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt cgaatggatc gctccagaga
     6841 ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc aaagtaattc acatctttta
     6901 aaatcttttc tgcttgttct ttggatgcct ttccatttct acgttcaaca aaagtatcca
     6961 ccatttcatc ccacttatcg taattatcag ggctgatgat cgtaaagcta gatgttttga
     7021 ttcctctggc atgcgctgca tttcttattt cttcttcgtt ccctactaaa atgggttcca
     7081 caagttcttc tgctttcaac cgggcagctg cgcctaaaat acgagcatca gtagcttccg
     7141 ggaaaacgat tttgatccca cggcgaacaa ttttaaatct taaactatca aacaattcca
     7201 caagatgacc tccagacgtt tttatatgta ttatcataca ctattccaga aaaaaagaac
     7261 agtttatttt atctgtatca gtcaaaaatg ctggaaaaat taagtttttc acatacaaag
     7321 cgaactgcac tataacagga aagcgctgtt ttattacact gtttcaggca gttgccaatc
     7381 aatcggttct tctcctaacg caaggagcgc atcattcgtt ttcgagaagg gtttggaacc
     7441 aaaaaagcca cgacttgcag ataacggact gggatgtgga gatgttatga tgatatggcg
     7501 actcttatcg atcattttga ttttttcttg tgccggtttt ccccacaaga taaacacgat
     7561 tggtttttcc cgttcattta atttttcaat gatcgtgtct gttaatcttt cccaaccttt
     7621 cccgcgatga gaatacgctt gcccttcccg aacggtaaga acggtgttga gtaaaaggac
     7681 tccctgttta gcccatgaca ctagatttcc atgttgaaca ggtgcgattc caagatcatc
     7741 ataaagctct ttatagatat ttcttaacga tggcgggatc tttacgccag gttgcacaga
     7801 aaaagataag ccatgagctt gattttctcc atgataagga tcttgtccta aaatcaccac
     7861 tttaacttct tcataaggag tcaattccaa tgcttcatag atatgataca tatctggatg
     7921 gatcttttgg gacgcatatt ctttttttaa aaattctctt agatgcagat aataatcttt
     7981 tgaaaattct gaggacaaaa cttcttgcca actgttatga atgatttctt tcaaaaactc
     8041 cacctcctaa taaaaagaat aactcacatt ttcttcaatc acaagggaca atcttcaccc
     8101 tcagcacttg attaatatgg taaactagga acaagaagca gaaaggaacg ataatcatta
     8161 tgatcaaatt aattgcatca gatatggacg gcacattatt agatgcccat atgagtgttt
     8221 ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc cggtgttgag ttcatggtcg
     8281 ctactggtcg aaactatcaa gaagctcgcg cagctttaga tgaggtagga atcgattgtg
     8341 cgatgatcac tttgaacggg gcacaggtat ttgataaaga aggaaattca ttgtttaccg
     8401 tgtcgattcc aaataatcaa gcaatctctg ttttagatat tctagatgct aacggtattt
     8461 actatgaagt agcaaccaac gatggtcttt attctgaaaa tcaagcgaaa cgaattgaaa
     8521 gttttgcttc catggtggct acccatttac ctcaccttac gtataaaatg gcgattgcca
     8581 tggcctcagc taagctggag ttattgcaca tcacatacgt ggacagtatt cgtgctattt
     8641 tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt tcatacagaa gggccagctg
     8701 tattaggtcc agttggaaag cagatttctc ttttaggcga tctagccgtt acttcctctg
     8761 gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa aggaattgct gttgcccatg
     8821 tcgctcacga gcgtgggatt tctttagaag aagtcatgac gattggcgat aacttcaatg
     8881 atgtcagcat gctgcaagta gcaggagtca gtttcgcaat gggaaatgca gaaatcgaag
     8941 taaaagatta tgccaaatat acaacggaca caaaccttga atctggcgta ggaaaagcca
     9001 tattgcgcgc gattgcagaa gacttgtagt tttatctaga aaagagggat atagatgtca
     9061 gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc ggacaattgt caaaaacgaa
     9121 aaaggacatt ccctgttttt gatggttggt cgctggggaa caaagggcga tgcattgtct
     9181 ctttatgcca tgaatggcga tctcgttgct cacatcaagc agataagcct gacattcggt
     9241 acgcgttttg agctgtataa agagttcgaa aaggtgggaa ccatgcagaa aatattcaat
     9301 tggcccggtg acttttatta tattcgtaga cttcactgga cagtccaagg tgatatctat
     9361 aatcatcaat atcaaatcca tcatttcaat cagcaaatca tgaaaatgga taaagcaaca
     9421 cttttttcag gagattacta tgtattggat gtctcaaatg atgaagatgc tccagtttgt
     9481 atttgtattg ctgcggtttt agattactgg ctctaccgta aagacaaaag tcgcgataag
     9541 cctgtttctt accgacttaa cttaagttaa aaagatgtac ctgaaatgcg gaaatacgca
     9601 tttcaggtac atctttttag atattctttt gcatatgatc ataaagatga ccactgatca
     9661 tccgctgacc gacaactttg aagtcatggc gttcgtataa ttttttagcc tttggatttt
     9721 tttcatccac acttagaccg atgacggagc gattagcttt cttcgccaat ttaggtaatg
     9781 cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc ttctgataca ctgatagagt
     9841 ccaaatacca ttcgttggga aacgtttctt tatcaatgaa caaacgtgta gacgcatcta
     9901 gctgatattt ctctaagata ggtaccagtg gctgatcgat gattgattct tcttcatctg
     9961 tataaccgta agcaactcct gcaactttac cgtctatttc ttctacgata cctcgtgaat
    10021 aactgtaacg ataatttgga tcagtgattg cttcttctaa aatctctaaa gtcttttgtt
    10081 cccctgtctg cttcaaaaat ggcagttcca tatcttttag aatcactaag atcaatgggg
    10141 cgatttctgg agcatcttct tttctcgcaa aacgaatcat agtttcctct ccttttctcc
    10201 attctaacat gcagaaaaag aaatcgcaaa atcgttattt taaaaaatct tcaatctttt
    10261 cagtaatgaa ataaccatga gtaacgattc ttgccgcttc agttcggcaa taagggcaga
    10321 cgactggcat ttctttatca ttatgttcga ctacattatt gacttcttcg tatgcaaatt
    10381 tttttccgca attagggcat ttcatttttt ccatgttatc tcaacttcct ttcatcttta
    10441 ctctcttagc ttactcttat attgtaaaaa agagaagcaa tatccttctt ctatgcagat
    10501 atccatacct gacggtcctg ttctccatct attaatcgga ctttataata aggacgttca
    10561 tctttgacta gaagtgtcca ctctttcact tttgcttttt tcccgtagta gctgactgct
    10621 tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt tttctatatc aaaaagatgt
    10681 tctgcggcct tttccgtgtc atttcgtacg gttgttttcc cagtggatgg attgactgct
    10741 atgacttgtc cttcacaaaa aaactggtat tcatatgttg cattcgtagg attctccaaa
    10801 tctttttcaa ctgcaaaatg aatagcagat atgttatgtt cttttgtttc gttttttacg
    10861 attgctgctg cttttttcaa tgagatccga tattcctggt cactaatatc atccatgtga
    10921 aaagcggcat tgagcagtcc cgtcataccg gtaatcccta ttacgatgag ggcagcaaca
    10981 ccaaacatca tttttttcat tcttcattct cctgtttctt ttttttaaag aatatcaaga
    11041 aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg acaattttgt tatctttatt
    11101 cgtgcagttc taatggcaat ccgtctggat caaagaaaaa cgtcattttt tttcctgtaa
    11161 aggtatcttc tctaatcggt tctgattcga tccctaagga ctgcaattct gcaattgttt
    11221 cttcaatatc gtctactttg aaagcaagat gacgcaagcc acaagcttct ggatagtttg
    11281 gtcgttgtgg cgcattagca attccaaaaa tctccaattc agcacttcct agtttcagat
    11341 ctaatttata gtcatctcgt tctttgcggt aattttctct gattacttca aatcctaatt
    11401 tattaacata aaaatcacgt gatttctgat aatctgaaac aatgatggct atatgatgga
    11461 tcgttgtaag gttcatatga gcactcctat tcttcgttta agctcggatc tatattaatc
    11521 tccagttcca ttgtaaatgt gtcagattga aaaaaatagc tgtttttcct ttttataaga
    11581 aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac tcttcctgtt cccaatataa
    11641 taaatgaaag taagaaatta tattttcaag aaaacaaaca gaaaattatc ataacacaaa
    11701 ttatttataa ttgaaagact agtttttttt caagaaaagc atacaataga ttgtgttagt
    11761 aaatacagaa cacactacat ttagtgtctt aaggagtcct catacatgaa gtcatatttt
    11821 cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa attattatct tttctttttc
    11881 agtcttggct gccaggtcat gactcttgtc aatagtccca tcacttggct gtccgttatt
    11941 acatttatcg gtaccacgct tggtgtattg tgtattttat ccatcaatgc ggcaaaatca
    12001 gtcaacggta ttttaggtat tttgtctgcg atttgtttta ttatcgttgg gttttcagca
    12061 aagaactatt taagtatcgg tgaacagatt gcttatatga tcacattgga tatccccgta
    12121 ttgttaagta aagaatggaa tcataatatg gcttcaaaaa tccgaaaatt cactggaaaa
    12181 agctggttta tcgcaatcat cagtactttc cttgtctatc ttgtttcagg attcctgatt
    12241 ggtcgttata cagatgatcc tcggccgtgg gtagatgcca ttgctttttc tattagcttg
    12301 actgctggta tcatcagttt cttgcgttac aacaaccaat attactggtg gttagcttca
    12361 gggcttgctc aaatggtcct ttggtatatt tcatttacgc atggatctgc aagtttggcg
    12421 atgttcgtga acagctctgt ttacttgctg aatgatgtcc ttgcatttac cgtttcacct
    12481 tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg aagaagaata ctacaacgca
    12541 caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta aagaacactt ttgtctcacg
    12601 ctctaaacac gtataaacgg cggaatcaga agcaactcct tcggaaataa gctgaaattc
    12661 acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc
    12721 tgtcccagcc tctatttttt atagtaattc cttcagatac tttcttgcct catcattgaa
    12781 acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag cttcagtaaa
    12841 acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag attggatatc
    12901 tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat cagcagattg
    12961 tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta tccgaggaat
    13021 gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa taaatagata
    13081 gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag cagtgacttc
    13141 tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa aataagcatt
    13201 aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta aaaatgcgcg
    13261 atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt cgtcaaatag
    13321 ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta ttttcttgat
    13381 ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt ggcgaaattc
    13441 tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc gacgattttg
    13501 gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat tcttctgctt
    13561 aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat ggatcgaagc
    13621 tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta gttttaggat
    13681 ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac cacactcaca
    13741 atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga agtcagaaaa
    13801 cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga ttagatatcg
    13861 gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga tctcccaacg
    13921 ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga tttcttttgg
    13981 aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc tttctaatta
    14041 caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc taaattagtg
    14101 agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt caactgcata
    14161 agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg gacgatcgat
    14221 cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg ggaattgtgt
    14281 ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc ctgctgtata
    14341 agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt tgattggcaa
    14401 tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg gctgatttcc
    14461 ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga acgagactgc
    14521 tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct gttggatggc
    14581 ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt tgatgatttc
    14641 agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat tcactttgtc
    14701 cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct ccgtccgatt
    14761 gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta aataaaggaa
    14821 acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa aaatagtgtt
    14881 ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc tcttctatta
    14941 tttaaataga atcatataag caatttggaa aactagcata aataacgcaa taaacaactg
    15001 gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca cgatattgaa
    15061 gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg caaccattcc
    15121 tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc caaccgtcat
    15181 cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca tgccaagagc
    15241 gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta ctgcattgga
    15301 aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta aaagcattgg
    15361 caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta aaaatgtact
    15421 tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa caccgacgcc
    15481 cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa agacaccgat
    15541 actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt ccatatattc
    15601 ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg gtatggtcat
    15661 cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg caataactac
    15721 ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt tatctgttaa
    15781 atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga tctccagtaa
    15841 tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc ccctttcctg
    15901 gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt gccacaacta
    15961 aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt acgtcatatc
    16021 ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg aatagtactt
    16081 ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt tcttcttcat
    16141 gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc ggtaaaatga
    16201 ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca aagatcattc
    16261 gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat cgcttgatca
    16321 aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc aacataatga
    16381 tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt tctaacattc
    16441 cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt acgatcaata
    16501 tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa aggctaagat
    16561 aattttccat atattttcct cca
//
LOCUS       DAYYJC010000055        16501 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000055 DAYYJC010000000
VERSION     DAYYJC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.055"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACHA9N_001751"
     CDS             complement(29..727)
                     /locus_tag="ACHA9N_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9085211.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACHA9N_001752"
     CDS             complement(720..2072)
                     /locus_tag="ACHA9N_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9085212.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACHA9N_001753"
     CDS             complement(2295..3695)
                     /locus_tag="ACHA9N_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9085213.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACHA9N_001754"
     CDS             3856..4593
                     /locus_tag="ACHA9N_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9085214.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACHA9N_001755"
     CDS             4718..5197
                     /locus_tag="ACHA9N_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9085215.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACHA9N_001756"
     CDS             5194..5961
                     /locus_tag="ACHA9N_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9085216.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA9N_001757"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACHA9N_001757"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9085217.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACHA9N_001758"
     CDS             complement(6864..7466)
                     /locus_tag="ACHA9N_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9085218.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACHA9N_001759"
     CDS             7572..7802
                     /locus_tag="ACHA9N_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085219.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACHA9N_001760"
     CDS             7793..8041
                     /locus_tag="ACHA9N_001760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085220.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACHA9N_001761"
     CDS             8147..8731
                     /locus_tag="ACHA9N_001761"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9085221.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA9N_001762"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACHA9N_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9085222.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA9N_001763"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACHA9N_001763"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9085223.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACHA9N_001764"
     CDS             10632..10811
                     /locus_tag="ACHA9N_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085224.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACHA9N_001765"
     CDS             10833..11858
                     /locus_tag="ACHA9N_001765"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9085225.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA9N_001766"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACHA9N_001766"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9085226.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACHA9N_001767"
     CDS             13430..14377
                     /locus_tag="ACHA9N_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9085227.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACHA9N_001768"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACHA9N_001768"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9085228.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACHA9N_001769"
     CDS             complement(15279..15521)
                     /locus_tag="ACHA9N_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085229.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACHA9N_001770"
     tRNA            15642..15714
                     /locus_tag="ACHA9N_001770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="ACHA9N_001771"
     CDS             complement(15833..16471)
                     /locus_tag="ACHA9N_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9085230.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5624 a         2947 c         3317 g         4613 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 a
//
LOCUS       DAYYJC010000056        16492 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000056 DAYYJC010000000
VERSION     DAYYJC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.056"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACHA9N_001772"
     CDS             <1..1452
                     /locus_tag="ACHA9N_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9085231.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ACHA9N_001773"
     CDS             1801..2433
                     /locus_tag="ACHA9N_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9085232.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ACHA9N_001774"
     CDS             3021..4460
                     /locus_tag="ACHA9N_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9085233.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ACHA9N_001775"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ACHA9N_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5222..5437
                     /locus_tag="ACHA9N_001776"
     CDS             5222..5437
                     /locus_tag="ACHA9N_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085234.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ACHA9N_001777"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ACHA9N_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ACHA9N_001778"
     CDS             complement(6097..7008)
                     /locus_tag="ACHA9N_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9085235.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ACHA9N_001779"
     CDS             complement(7027..8031)
                     /locus_tag="ACHA9N_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9085236.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ACHA9N_001780"
     CDS             complement(8028..10151)
                     /locus_tag="ACHA9N_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9085237.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ACHA9N_001781"
     CDS             complement(10156..12603)
                     /locus_tag="ACHA9N_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085238.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ACHA9N_001782"
     CDS             complement(12590..12979)
                     /locus_tag="ACHA9N_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9085239.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ACHA9N_001783"
     CDS             complement(13039..13542)
                     /locus_tag="ACHA9N_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9085240.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="ACHA9N_001784"
     CDS             complement(13555..13779)
                     /locus_tag="ACHA9N_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085241.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACHA9N_001785"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="ACHA9N_001785"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9085242.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DAYYJC010000057        16275 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000057 DAYYJC010000000
VERSION     DAYYJC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.057"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..553
                     /gene="hslV"
                     /locus_tag="ACHA9N_001786"
     CDS             5..553
                     /gene="hslV"
                     /locus_tag="ACHA9N_001786"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9085243.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            567..1967
                     /gene="hslU"
                     /locus_tag="ACHA9N_001787"
     CDS             567..1967
                     /gene="hslU"
                     /locus_tag="ACHA9N_001787"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9085244.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1985..2776
                     /gene="codY"
                     /locus_tag="ACHA9N_001788"
     CDS             1985..2776
                     /gene="codY"
                     /locus_tag="ACHA9N_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9085245.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2858..3733
                     /locus_tag="ACHA9N_001789"
     CDS             2858..3733
                     /locus_tag="ACHA9N_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9085246.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="ACHA9N_001790"
     CDS             complement(3865..4503)
                     /gene="plsY"
                     /locus_tag="ACHA9N_001790"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9085247.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4630..5064
                     /locus_tag="ACHA9N_001791"
     CDS             4630..5064
                     /locus_tag="ACHA9N_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9085248.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5230..7275
                     /gene="parE"
                     /locus_tag="ACHA9N_001792"
     CDS             5230..7275
                     /gene="parE"
                     /locus_tag="ACHA9N_001792"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9085249.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7287..9737
                     /gene="parC"
                     /locus_tag="ACHA9N_001793"
     CDS             7287..9737
                     /gene="parC"
                     /locus_tag="ACHA9N_001793"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9085250.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10014..12245
                     /gene="pflB"
                     /locus_tag="ACHA9N_001794"
     CDS             10014..12245
                     /gene="pflB"
                     /locus_tag="ACHA9N_001794"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9085251.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12489..13250
                     /gene="pflA"
                     /locus_tag="ACHA9N_001795"
     CDS             12489..13250
                     /gene="pflA"
                     /locus_tag="ACHA9N_001795"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9085252.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13335..14261
                     /locus_tag="ACHA9N_001796"
     CDS             13335..14261
                     /locus_tag="ACHA9N_001796"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9085253.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14443..15336
                     /locus_tag="ACHA9N_001797"
     CDS             14443..15336
                     /locus_tag="ACHA9N_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9085254.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15470..15595
                     /locus_tag="ACHA9N_001798"
     CDS             15470..15595
                     /locus_tag="ACHA9N_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9085255.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15729..>16275)
                     /locus_tag="ACHA9N_001799"
     CDS             complement(15729..>16275)
                     /locus_tag="ACHA9N_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085256.1"
                     /translation="YLKTSHAPSSALGWLLLDHSDITEAEENPFFLILSSLCGLLFSV
                     YFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFIMNGLVVILSIFT
                     YFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQVAGNLLDMLFI
                     VSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5770 a         2778 c         3478 g         4249 t
ORIGIN      
        1 aaacatggtc gaatcaccat ttcattcaac cactatctgt gccgtcgaaa aagacgggaa
       61 atttgctatg gctggagatg gccaagtaac aatgggtgaa tctgttgtca tgaaaggcac
      121 tgcaaagaaa gtacgacgta tctacaatgg agaagttgtt gtaggtttcg ccggaagtgt
      181 tgcagatgcg tttactttag aagaaaaatt tgaaggcaaa ctaaacgaat ataacgggaa
      241 tctacaacgc gcagctgtcg aattagctca agaatggcgg acacagcaat ctatgcaaaa
      301 acttgaagcg atgctgatcg taatgaatga taaagaaatg cttcttgttt caggtacagg
      361 agaagttatc acaccagatg atggtatctt agctatcgga tcaggaggga attttgcgtt
      421 ggctgcagcc cgagcaatga aaaactatgc tcaaaacgac atgagtgcga aagaaattgc
      481 agaaaatgcg ttaaatattg ccgcagatat ctgtatcttt actaatcata atattatcgt
      541 agaagaactg taagaggtga gtagatatgt atgaattaaa acaaacacca agacaaatcg
      601 taagtgaatt agatgaatat atcgtaggac agcacgcagc aaaaaaatct gttgccgttg
      661 ctttgcgtaa ccgctatcgc cgtttgcagt tagatgaaaa aatgcaacaa gatattacac
      721 caaaaaatat gctgatgatc ggacctacag gggtcggtaa aacagaaatc gcacgacgtt
      781 tggcaaaaat cgtcaatgca ccatttgtca aagtcgaagc aacaaaattt actgaagtcg
      841 gttatgtcgg aagagatgta gaatcaatgg ttcgggattt agtagaaaat gcgatccaaa
      901 tcgtagaaaa agagcagtac agccgtgtat acacaagtgc taaaaaaaat gcagaaaaac
      961 gtttggtaaa aatcttggta cctggtatca aaaaagaaca gaaacaatcc actaatcaat
     1021 ttgaacaaat gatgaatctt tttaatgcta gtcagcaaaa taccgctgaa ccgcaagaag
     1081 aagtgacaga agatattcga atcaatcgcc gtacagtctt ggaacaacta caaaagggtt
     1141 tactggatag tcgtgaagta accattgaag ttgaagacca aaagaaacct gcaccaatga
     1201 tgaacaatgg attggagcaa atggggatcg acctaaatga aactctaggt gctttgacac
     1261 cgaagaaaaa agtggaacgg acggtaacag tcaaagaagc tttagagtta ttgatcaagg
     1321 aagaatcagc aaaattagtc aaggatgctg atatccatag tgaggcaatc aaattagcgg
     1381 aagaaagtgg aattattttt atcgatgaaa tcgataagat cacttctaaa tcacagcaat
     1441 ctggagaagt atccagagaa ggcgtccaaa gagacatatt gccaattgtc gaaggttcac
     1501 aagtaaacac aaaatacggg atcatccaaa cagatcacgt cctattcatt gcttcaggag
     1561 ctttccattt gtctaaacca agtgatctaa ttccagaact tcaaggacgc ttcccgatac
     1621 gggtagaatt agatgatttg acagctgaag attttgtgaa gattctgacc gaaccaaata
     1681 atgctttagt aaaacaatac gtcgcgttga tcggtacaga aaatgtgtca gttacgttta
     1741 caaaagaagc aattgaacgg atcgctcaaa tcgctttcga tgtcaaccgt gacacagata
     1801 atatcggcgc acgcagactg cataccattt tggaaaaact actagaagat ctattatttg
     1861 aagcaccaga tatgcaaatg ggagaaataa cgatcacaga agcatatgtg gatgacaaac
     1921 ttggctcaat tgcggcaaat gaagatttaa gtcgctatat tttataaaaa atcggaggac
     1981 gacaatgaca gacttgctaa cgaaaacccg caagataaac acacttttgc agcaaaagaa
     2041 tacttttgac atgcaagcag atttgcctta cgataaaatg gctgtcacac taggtggtat
     2101 tcttgacagc aacacctata tcataggaag tgacggagta ttactaggtt ataatgaacg
     2161 tcacgatgtg aacaatgaac gaatcaaaac aatgtttgtt gagaaacaat ttcctgaatc
     2221 ttatactgat acagtggcac agttgatgaa aactgaagct aatatcccaa ttactagtga
     2281 tattactgca tttccggtag agtcaagaga gaaatatcca tttggtctga cgactgtcgt
     2341 tccgatcttt ggagctggcg aacgtttggg tacgattatc ttatctcgta tggaacaatc
     2401 gtttgcagat gacgatctag tcttagcaga atatggagct acggttgtcg gtatgcagat
     2461 cctttatcag aagtctcgaa gcatcgaagc agatgtacgg agtgcaacgg ctgtccaaat
     2521 ggcaatcaac acattatctt atagcgaatt gaaggctgta caagctatct ttgaagcatt
     2581 ggatggagat gaaggtcgtc tgactgcttc taatattgcc gatagtatcg gcatcactcg
     2641 ttctgtgatc gtcaatgctt tacgaaaatt ggaatcagct ggaatcattg aatctcgttc
     2701 gttaggcatg aagggaactt atttaaaagt attgaattcc cgttttaaag acgagttagc
     2761 aaaacacagt tattagaagt aaggggactg gggcagatag aaatgtttgt tccagccttc
     2821 ccattttaaa aagtaagtcg aagttggagg aatggcaatg actgttatta ttcaaaatga
     2881 ccagctgatc gccgaaatcg ctgaacacgg tgcggaattg atcagtctga aatcaaaaga
     2941 acatggttta gagtatatct ggcaaggtga tccagcgtat tggggcagac atgcaccggt
     3001 tttattccct tttgttggac gtttaaaaga tgatcagtat gtttatcaag ggaaaacata
     3061 tcaaatgggg caacacggat ttgctcggga tatggatttt gaagtcattg aacatggaca
     3121 agaaatggct agtttcatct taaggagtac gccggagaca ttagaaaagt atccatttga
     3181 ttttgaatta gtgatcagct atgaattagg aggagacggc atcacggttc attatcaagt
     3241 ggcaaatacc ggtgaaaaag aaatgttctt ttctatcggt ggacatcctg ctttcaatgt
     3301 acctcttgaa gaaggattgt catttgaaga tttctatctg gcattttctc cattgaaatc
     3361 aagattacgc ctaccattga aaggcccatt catcgatatg gatcaaaaga cgttaggtca
     3421 gacaaatacg aatctggctt tgaatcgtga actttttgag caagatgcct tggtttttga
     3481 aacaaggggt ctaaatgcgt acgccattcg aagtgaaaaa tcttcccata gcgtaacttt
     3541 aagctataaa gatatgcctt atgtagggat ctggtctcct tatccaaaag aagcgccttt
     3601 cgtttgtatc gaaccgtggt gcggaatcgc tgacacggtg aacagtagtg gaaacttgat
     3661 ggaaaaagta gggatcaatc aactaggtgc ccatgaaatc ttcaagacaa aatattccat
     3721 cacagtgaag taagaggttg tgaccgagaa gttagcaggg aacaccgttt accaaggtgt
     3781 gtaaaaaagc tatcctgtaa gtaataaggg agtgtgacat cgatttgtca cactcccgtt
     3841 ttatctcttt ggaatgaaaa agtatcagga ttttttcttg ctgctattca aaccaaacgg
     3901 gattcgactt tcagtatggt ttctgatacg cttgatattg tctttatgcc gataaaagat
     3961 aaaggaagaa actaaaaagg caatgatcgt cagtaaccaa ttgggcttag ccaaaatcac
     4021 aggagctaca taaggaagga cgatcgtaga taaagtgatc aatactgcac tgatcatact
     4081 gctcaaactc accatacttg tacaaaataa actaacgata aagatgatac aagaataaac
     4141 aaaaaataca ggattaaatc ctaaaagcat tcctgcactt gtcgcaacag cttttcctcc
     4201 tttgaaccca gcaaaaatcg gaaatgtatg gccaagaatg gcgcataccc caaaccaaag
     4261 agggttaatc ccagaaaggt ggaagatcat cggcaaacaa gtagccaatg taccttttaa
     4321 aatatccata agcaggacaa caatcccggc tgttttgcct aatacacgaa atgtattggt
     4381 cgttccagta ttaccgctcc cgtgttgtcg gatatctttt ttgaaaaaca atttgccaat
     4441 ccagacaccg gaaggaatag aacctaaaag atatgctaaa ataagtaata agatcaattt
     4501 cattttttac cctctttcaa gttgaaaact atcctttatt ttagcatgaa taggtaacag
     4561 gaacaattga aaaaatgaaa gatacaagta taatagatgg aataaaaaga aaagcaaggg
     4621 gtggcagaaa tgtctgtaaa aaatccaaca caagaaacga ttttcaacta tttggaagaa
     4681 gcaaaaaata ttgccgtcgt aggcttgagc aataaaccag aacgaacaag ttacatggtt
     4741 gcacaggtat tgcaagacaa tggatataaa atcataccag taaatccatt attggctggt
     4801 gaagaaattc taggcgagaa agtctatgcg aatttgacag aaatcgatga tccaatcgat
     4861 atcgtagatg tatttagacg gagtgaattt ttacctgaaa tagcaggtga ttttttgaaa
     4921 actgatgcta aagtgttctg ggcacaatta gggattgaaa acaaagaagc agctgagatg
     4981 ctaagaaaag caggacgtac agatatcgta atggatcgtt gtattaagat tgaacttggt
     5041 aagttgaaag aaaaccaaaa ataaaaatga ccacaaagaa gagatgatcg atagagggga
     5101 atgtcgaaaa agcggttagg agataattaa aattgatttc tcgtctaccc tctttttttt
     5161 cacgaccttt tctagtatga taatcgtggt gcggttttgc cgcaaagtat tattgaagga
     5221 gcgtttgcct tggcaaaaaa agtaaacaac gaatacaatg actcgtccat ccaagtattg
     5281 gaaggattag aggcagttag aaaaagacca gggatgtaca ttggatccac tgacagccga
     5341 ggcttgcatc atttagtcta tgaaatcgtc gataatgcag ttgacgaagc attatcaggt
     5401 tatggcagtg agattgatgt gaccatccat gaagacaata gtataactgt tgcagatagc
     5461 ggacgtggga tgcctgtagg aatgcatgct tccggaatac caacagttga agtgattttt
     5521 actgtgctgc atgctggcgg aaagtttggt caaggagggt acaaaacttc tggtggactg
     5581 cacggtgtag gagccagtgt ggtaaatgcc ttatcgaaat ggctaattgt aacgatcgtt
     5641 agagatggcg tagagtatca gcagaaattt aaaaacggcg gtaaacctga cggtactttg
     5701 aaaaagatag gaaaaacaaa aaaagcaaac gggacgactg tccatttttt accagatgat
     5761 acaatattca gtacaacgaa attttcatat gaaattttag ccgaacggtt aagagaatct
     5821 gcctttcttc taaaaggcgt taaaatctct ctttctgatc ttcgtggaga agaaccagtc
     5881 aaggaaattt ttcattacga agaaggaatc aaagaatttg ttgattatct gaatgaagaa
     5941 aaagatacat tgacaccagt tgtttatttt tcaggagaaa aagaaggtat cgaagtagag
     6001 gtcgcttatc aatacaatga cggttattca gaaaatgttt tatcttttgt caataacgtt
     6061 cggacaaaag atgggggaac ccatgaagct ggtatgaaag cggcgatgac gaaatcttat
     6121 aatgaatatg cgagaaaagt tggtttgctg aaagaacggg acaagaatct ggaaggtagt
     6181 gatttcagag aagggttagc tgctgtactt tctatccgtg tacctgaaaa tcttctgcaa
     6241 tttgaagggc aaacaaaaga aaaacttggt acgccagttg ctcgtaccgt agtagataac
     6301 gtcatcagtg aacaaatggg cttttatttg caggaaaata gcgaaatgag ccagatgttg
     6361 gtccgtaaag caatcaaagc cagagaagcg agagaagcag cacgtaaagc cagagaagaa
     6421 agccgaaatg ggaagaagcg taaaaaaggc gaatctcttc tttcaggaaa attgacaccc
     6481 gcacaatccc gaaaccctaa gaaaaatgaa ttgtatttag tcgaagggga ttctgctggc
     6541 ggatctgcta aacaaggaag agacagaaag ttccaagcaa ttctaccttt acgaggaaaa
     6601 gtcatcaata ccgaaaaagc taaaatgcaa gatatcttga aaaatgaaga aatcaatacg
     6661 atgatctata cgattggtgc aggagtcggt cctgagtttt ctatcgaaga ctgtaattac
     6721 gacaaagtaa tcatcatgac cgatgcggat acagacggcg cccatattca agtattactg
     6781 cttactttct tttatcgtta tatgaaaccg ctgattgaag ctgggaaagt atatatcgcg
     6841 ttaccgcctt tatataaagt atccaaagga caaggaaaga agcaagtcat tgaatatgcg
     6901 tggacagatg acgaattggc agcaatgatt aaaaaagtcg gcaaaggata tatgctgcag
     6961 cgatataaag gacttggaga gatgaatgcg gaacaattgt gggaaacaac gatggatccg
     7021 acttcccgta cgttgatccg cgtccgtatc gatgatgctg cgcaagctga acgtcgagta
     7081 accacactga tgggtgacaa agtagaacct cgaagaaaat ggatcgaaaa tcacgtgcaa
     7141 tttacgctcg aagaagatgg cagtatctta gataaaaaag aggatactga aatctctcct
     7201 tcagtttcta atgatttatt agatgaagaa cgagcagata aaaatgaaaa caatcagtta
     7261 tttgaagtag aataggaggt cacctgatgg aaaatcgaca agaagtacaa gaattaactt
     7321 tagaagaagt gatgggtgac cgcttcggac gttattccaa atatattatc caagaacgtg
     7381 cactgcctga tatccgtgat ggattgaagc cagtgcaacg ccggatatta tttgccatga
     7441 acaaagatgg caatacgtat gacaaaggat tccgtaaatc agcaaaatct gtcggaaata
     7501 tcatggggaa ttatcatcct catggtgaca tcagtattta tgaagcgatg gtccgcatga
     7561 gccaagactg gaagttgcgg gaagtactga tcgaaatgca cgggaataac gggagtatgg
     7621 acggtgatcc acctgctgcg atgcgttata ctgaagccag attatccaag ttaagtggag
     7681 agatgctagc agatatcgag aaagaaacag tcgatctcgt gtggaatttt gatgatacag
     7741 aaaaagaacc aacagtcctt ccagctcgat acccaaacct tctcgtcaat ggatcgacag
     7801 gtatatctgc tggatatgca acagaaatcc cgactcataa cttagctgaa gtgatcgatg
     7861 gcacaatcta tatgatcgat catccacaag cgagccttga aaaattgatg gaatatattc
     7921 ctggacctga ctttccaaca ggagggatct tgcaaggaaa agatgaaatc aaaaaagctt
     7981 atgaaacagg acgaggaaaa gttattcttc gttcgaaaac aaagatagag gcaatgaagg
     8041 gtaataaaca gcaaatcgtg atcacagaaa tcccatatga agtaaataaa gcgaccttgg
     8101 tgaaaaaaat ggatgagatc cgtttgaata agaaaatcga tggaatagct gaagtacgag
     8161 atgaaagtga tagaacaggt ctgcaaattg tcgtcgaatt gaaaaaagac gccaatgctc
     8221 aaggaatttt gaattatttg ttcaaaaaca ctgagctaca gatcaattac aatttcaata
     8281 tggtcgctat cgaccatatg actccgcatc aagttggact gaaagacatt ttaagaagtt
     8341 atatcgagca tcgaaagcaa gtcataacaa aacgtagtca attcgattta gccaaagctc
     8401 aaaaaagaca gcatatcgta gaaggattga tgaaagcttt atcgattctg gatgaagtaa
     8461 tcgcaacgat tcgtgaaagt aaagacaaaa aagacgccaa aaagaattta gtcgacgtct
     8521 ttcaatttac agaagaacaa gcagaagcaa ttgtcacatt gcaattatac cgtttgacga
     8581 acacggatat cacagagctt caaaaggaat ccgaaagctt gattgcgcaa atcacagagt
     8641 tgaataaaat cctttcaaat gacaaagagc ttttctctgt tatgaaaaaa gaattgcgag
     8701 aagtgaaaaa aaattattct tccgctcgtc taacgacaat cgaagacgaa atcgaagaaa
     8761 tcaaaattga tacgcaagtc ttagtggcac aagaagacgt aattgtcagt gtgacgcgag
     8821 aaggctatgt gaagcgaaca agccttcgtt cttatagtgc ttccaaacca gaagaaatcg
     8881 ggatgcgaga aggcgactac ttgctatact caggtgaatt gagtacattg gaccatgttc
     8941 ttctgatcac aaataaagca aatgtgatct accgccctgt ccatgaacta cccgatttga
     9001 aatggaaaga tgctggtgag cacatttctc aaacaatctc caatctgtct gtagacgaat
     9061 cgattttagc tgttttccca tatcaaaaaa ttcaagcaga gaaaacattt gtattcatca
     9121 gcaagaatgg attgatcaag caaacacgaa tgacagagtt cgaaccatgg cgcacctata
     9181 agagccgtcc gctcagctgt atgaagctaa aggcggaaga agatgaactg attgctgtgt
     9241 atctgaaaga agatcagcca ggcttagatg ttttcttagt aacgcatcaa ggaatgggtt
     9301 tgcgctatcc tttaacagat gttcccgttg ttggagcaaa ggcagctgga gtgaagtcta
     9361 tgaatttgaa agatggagat aggattatca acgggctgct tgtgttagca gaaggtgata
     9421 cgccgatcgt aatcgttact cagcgaggag ctgttaaacg catgcttgct caagaaatca
     9481 gccaaacttc acgtgcgaag cggggagtga cggtcatgcg tgagctgaaa aaaaatccgc
     9541 accgtattat ctatatgtcg gaaggaagat caaaagaaat aaccttgatg aatcaaaaag
     9601 ggcagcagtt aacgattgac ccaactgatt tccctatagg tgatcgaaca tcgaatggat
     9661 catttgcgat ggatgagaaa aaaggtggag aagtcatcga agttatcgat gctcctgaga
     9721 ttttcatcga gtcgtaactg ttgtagtatt ttaatgaaga gacgccatct gtattaatac
     9781 aggtggcgtc tctttttcat tcatttttaa taaatcctta tacgtttgca aaaaaagatg
     9841 aaagtgttca caatgcttaa atgttggtat gtcaacgttg atttaaatat atgtaaataa
     9901 aacataaaaa ttgttaaata aaaaacaaga aaacccttgc aaagataggg tgctgtgtta
     9961 tactaatcac gtaaaaactg ttacataaaa cagaaattta ggaggtccat acaatggagc
    10021 aatggaatgg atttaaaggt accaaatgga aagaaggcgt agacactaga gactttattc
    10081 aaaataacta cactgaatat cgcggtgatg acagcttttt ggaaggccca acagaagcaa
    10141 cagaaaaatt atgggatgaa ttgcaaaagt taaatgatat ccaattcgag cgtaacggtg
    10201 tctatgatat ggacacagaa atcgtatcaa ctattacttc acacgatgca ggctatttgg
    10261 ataaagattt ggaaaaaatc gtcggtctac agacagacaa acctttgaaa caagcattca
    10321 tgccgttcgg cggaatcaat atggctaaca atgcgctaaa aagcaatggc tatacaccaa
    10381 acgatgaatt gacacatatt ttcactgact ggcgcaaaac acataatcaa ggtgtatttg
    10441 acgcttatac tgctgaaatg cgtgctgctc gtaaaaacaa aatcattaca gggcttccag
    10501 acgcttacgg tcgcggacgt atcatcggtg actatcgtcg tgtggcattg tatggaatcg
    10561 actatttgat ggaacaaaag aaaaaagatc atgatatgac tggttatcat acaatgagtg
    10621 aagaagtgat tcgtttacgt gaagaaatca ctgaacaata tcgtgcatta gcgcaaatga
    10681 aagaaatggc tgcgaaatat ggctatgata tttctaaacc agctgcaaat gcaaaagaag
    10741 ctgtccaatg gctatacttc ggatatttag cagcaatcaa atcacaaaat ggtgctgcga
    10801 tgtcaatcgg acgtatctca gcattccttg atatctatat ccaacgtgat ttggataatg
    10861 gagtaatcac agaaaaagaa gcacaagaat tgatcgacca cttagtaatg aaattacgta
    10921 tggtcaaatt cgcgcgtaca cctgaatata accaattatt ctcaggattc cctatctggg
    10981 ctactttatc cattgcaggt atgggattag atggtcgttc attagttaca aagaatgatt
    11041 tccgtctgct tcatacatta acaaatatgg gaccttcacc agaaccaaac ttgactgttt
    11101 tgtattctga acaccttcca gaaggattca gaacatatgc tagcagaatc gctatcgaaa
    11161 gttcttcgat tcaattcgaa aatgataatc agcttcgtga acaatggggt tctgatgatt
    11221 gtgcgattgc ttgctgtgta tcaggaacag ttgtcggaaa agacatgcaa ttctttggcg
    11281 ctcgtgctaa cttggctaaa acactattat atgccatcaa tggcggagtc gatgaagtga
    11341 ctggcgccca agttggacca gaatatcgtc caatcacatc agaaatacta gactttgaag
    11401 aagtgaaaca taacttcatg aatatgatgg attggttagc agaactatat gtcaatacgt
    11461 tgaacatcat tcattatatg catgataaat attcttatga agcagctcag ctggctttga
    11521 tggattctga cttgcaacgc acatttgcta caggtattgc tgggatctct catgcggcag
    11581 acagcttgtc agcgatcaaa tatgcaaaag taaaagcaat ccgtaatgaa aaaggaatag
    11641 cagttgattt tgaagtggat ggcgacttcc ctaaatacgg aaatgatgat gaccgtgcag
    11701 atgaattagt ttcatggatc ttggaatact tcatgggaca aatcaaacgt cataaaactt
    11761 accgcaactc aaaaccaaca acttcattat tgacgattac ttcaaatgtt gtttacggta
    11821 aaaatacagg aaatactcct gatggccgtg aagctggaaa accacttgcg ccaggagcaa
    11881 atccaagtta caatgcggaa caaagtgggt tattagctag cctgaactca acagctaaaa
    11941 tgaattacgg ctgtgcattg gatggaattt caaataccca aacaatcaat ccttcaggtc
    12001 ttggaaaaga acaagataca agaatcgaaa acttgcgtaa tgtcttagat ggatacttct
    12061 caaaaggagc ttatcatttg aatgtcaatg tattcagtaa tgatttattg cgtgatgcaa
    12121 tggaaaatcc aatgaaatat ccgaacttga cgatccgggt atctggttat gcagttaaat
    12181 tccgtgactt gacaagagaa caacaattag atgtcttgat gagaacttca cacgacagaa
    12241 tgtaaaaagt tcgtgaaaaa agtttttcga cttagcgtcg aaaggacgtt gaatagccat
    12301 cgcatcaaaa tcgaataatc agtatgctta tttgaaagaa acgatttaca ggggcaggac
    12361 ttgtatgttc cgtccctgta aattttacac ataacgaagc ggcaattgcg aaaaattttg
    12421 ttcagaaagc ttcgcttcga cagctaactt gtacttaaaa tgaaatcaaa gaaaagatgt
    12481 gaacttaaat ggaagaaaaa acaatcggct atgttcattc tatcgaaact ttcggttcag
    12541 tagacggacc tggactgcgc tttgtcgtgt ttatgcaagg atgccgcatg cgttgccaat
    12601 tttgtcacaa tccagatact tggaatattg gcggagggaa agaatacaca gcagatgaac
    12661 ttttagataa agcagaacgc ttcagacctt actggggaga taaaggtggt attactgtta
    12721 gtggtggaga acctctattg cagatcgatt ttttgatcga actatttaaa aaagcaaaag
    12781 agcggaagat gcatactaca ttggatacat gcggaaaacc ttttacgtat gaagaacctt
    12841 tcttttcacg ttttcaagaa ttgatgaagt atacagattt actgttattt gacatcaaac
    12901 acatcgataa tgaagagcat aaaaaactaa cgcattggga caatgaaaat attttagaaa
    12961 tggctcaata tttgtcaaaa atcaacaaac cggtttggat tcgccatgta ctagttcctg
    13021 agcgaagtga ttatgatgaa tatttgatcc gactagacaa ctttattcaa accttgtcaa
    13081 atgtagacag agtagagatt cttccttatc atacgatggg aaaatacaag tgggaaacac
    13141 ttggtttgaa atatccatta gaagggatcg aaccacctac aaaagaacgt gtcgaaaatg
    13201 ctaaacgatt gcttcataca gaagagtata caaaatattt aacgcgatag gtcgcatcgg
    13261 aaagaagata agtattgctt ctttccgttt ttttgctatt attaaatagg agaaatcaca
    13321 aaggagcgaa tgacatgtca aaaattttag tttttggtca ccaaaatcca gatacagatg
    13381 caatcggggc agccattgcg ttttcacact tgcaaaatga attaggtaaa gaaacagaag
    13441 cagttgctct aggtacacct aatgaagaaa cacaatacgc attagaccac tttcagctat
    13501 ctgcaccacg agtagtagag tcagctaaag cagaaaatgc agatgtcatg cttgtggatc
    13561 ataatgaatt ccaacaaagt atttcagata tcagcgaagt aaatatttta gctgtagtag
    13621 accaccatcg tattgcaaac tttgaaacag ctaatccatt gtactatcgt gcagaaccag
    13681 ttggatgtac aagtacgatt attttaaaac tatataaaga aaaccaagta acaattccta
    13741 aagaaatcgc aggaatcatg ctctcagcta tcatttcaga tacattgttg ttcaaatcac
    13801 caacatgtac gcctgaagat gaagcagctg caaaagaact tgcagcaatc gctgaagtcg
    13861 atctaaacac ttatggactt gaaatgttga aagcaggaac aaatctaagt gataaatcag
    13921 ctgcggttct tcttgatttg gatgcaaaaa gtttcccaat gggcagttcc aacgttcgta
    13981 tcgctcaagt caatacagtg gaccttaatg aagtgatgga tcgacaagca gagctagaag
    14041 cagcaatgga agatgaaatg aaagcaaatg gctacgatct gttcgtatta gttgtcacaa
    14101 acatcttaga cagtgattca gaattacttg ttgctggtaa aggaaaagaa aaagtagaag
    14161 aagctttcca aacaactttg accaacaacc gtgctttgct taaaggtgtc gtttcacgta
    14221 aaaaacaagt cgtacctcaa ttgacggctg tatttagcta atcaaatggt agtatgaagc
    14281 attcaaaatg aatggataag cgattgatat aaaaagaaac atagaacata ctaaaggccg
    14341 aagaaatgga ggagaataac agtcaattat gactcttgtt cttcctcctt tttacgtaaa
    14401 agccttttaa gtaaaataaa acggatagaa aagagaacat atatgaacta ttattcaaaa
    14461 aataaggaaa acttttatca aaaactgacc gatgatccgc ttttttcttc attgaccgat
    14521 tatctatatg aacatcgaga acaagaaacg attctaagag aattgaaaaa agaattttcg
    14581 caaaataaat tttcccattt tcttgattta ttgatcgatg caggattaat caagagggaa
    14641 gaacgaagat atcatttgaa ctttccaata tttgatccta aagattactt gcaacaagcc
    14701 acttcggctg ctgaaaccat agctgaacaa ttgaagagac tctctgttga tgaacaaaaa
    14761 ttagcgatgg gagaagttat ttgggcgtat tgtttcgaag atgaacggaa agaagcgtat
    14821 ttttatggag taagaaacag ccgagagaca gaattgctga ggacaactgc aggaaatgag
    14881 aagtaccgtt ttatcacact ttcctctata gaacacttcc cgcttacttt ggctaattac
    14941 ttttttgtac agaagaatca gcttcctgta acgaaggcat tcaaagaatt agccgaactt
    15001 attggagacg taaatgaatc gtactttttt gatcaaatag aagttatcgt tgacaggata
    15061 cgtaagaata aatataaaaa tcgccggcct agtattttcc atcagtctct acttgtgacg
    15121 aatactataa aggaagaaga atcattcaca ttagaattac ctattgtcga aaaaaacaat
    15181 tttgagattg aattaccaac attggatcct tcattgacaa tggaggaaat ggcctttctt
    15241 aaacgacaaa tcttttctga gctgagcaag aaatttatac cgcatgcatt ttcttatatc
    15301 aaagaatatg gaacagtttt agtgagtaaa acctaagaga gcagtaagca aaaattatac
    15361 tgactcttgc aggaggagcc tttctcttcc tttatggtta aaagaattat ttgagtattt
    15421 tgcctatcta ccacctatta gattcactac atggtctgga aacaaaggaa tgcaaaaaaa
    15481 tggtgagaaa tgtggaatga caaaggaagt agtgataaga aaagttagat ttttaaataa
    15541 tcaatattat gattcagtca agtatggcat tttatgggaa gaactagctg attagattag
    15601 gaagtccatt tgataatcca gaatcactaa gaaacgaatg gcgagtcatt aataatttat
    15661 ccaatcttgg gttattcttt tagattgaag tgtttaaaac taaaaaagca tttatgaagt
    15721 cgtaatagtc aggcttcaaa atgcttttta ttttgtttgt tttttctttg ccacataaat
    15781 acacctagac tgacgatgaa gagcatatcc aacagatttc ctgcgacttg gatcgtcgaa
    15841 gggttggtca gtggcattaa agcaaggaca aaaaaggaaa acaagctgta aataaaacag
    15901 gtgacaggaa ctaatcgaaa gaagagcgga catttcaatt ggctaaaaat gagtgaaaaa
    15961 ataaaataag taaaaatgga taaaatcacg actaaaccat tcatgataaa gaagaaaagc
    16021 gtattgtgat taaacgaata aaaaagaatt gctaaaaaag aaaacgtttt aaatgcaatc
    16081 aatacgcttg aagcttcgtt taacgtacca ggatgtcgat gaaaaaaata ggcaccgaaa
    16141 taaacagaaa acagtaagcc gcaaagagaa gacaaaatca aaaaaaacgg attttcttcg
    16201 gcttctgtga tatcagaatg atccaaaagc aaccagccta atgcactaga aggcgcatgt
    16261 gaggtcttaa ggtat
//
LOCUS       DAYYJC010000058        16092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000058 DAYYJC010000000
VERSION     DAYYJC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.058"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACHA9N_001800"
     CDS             226..894
                     /locus_tag="ACHA9N_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9085257.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACHA9N_001801"
     CDS             complement(1094..1480)
                     /locus_tag="ACHA9N_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085258.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACHA9N_001802"
     CDS             complement(1452..2834)
                     /locus_tag="ACHA9N_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9085259.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACHA9N_001803"
     CDS             complement(2990..4177)
                     /locus_tag="ACHA9N_001803"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9085260.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACHA9N_001804"
     CDS             complement(4184..5215)
                     /locus_tag="ACHA9N_001804"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9085261.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACHA9N_001805"
     CDS             complement(5231..6685)
                     /locus_tag="ACHA9N_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9085262.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACHA9N_001806"
     CDS             6860..7765
                     /locus_tag="ACHA9N_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9085263.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACHA9N_001807"
     CDS             7856..8533
                     /locus_tag="ACHA9N_001807"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9085264.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACHA9N_001808"
     CDS             8530..8700
                     /locus_tag="ACHA9N_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085265.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACHA9N_001809"
     CDS             8814..9710
                     /locus_tag="ACHA9N_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9085266.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACHA9N_001810"
     CDS             complement(9765..11135)
                     /locus_tag="ACHA9N_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9085267.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACHA9N_001811"
     CDS             11373..12119
                     /locus_tag="ACHA9N_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9085268.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACHA9N_001812"
     CDS             12116..12955
                     /locus_tag="ACHA9N_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9085269.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACHA9N_001813"
     CDS             12956..13906
                     /locus_tag="ACHA9N_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9085270.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACHA9N_001814"
     CDS             14047..14799
                     /locus_tag="ACHA9N_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085271.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACHA9N_001815"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACHA9N_001815"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9085272.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5219 a         3009 c         3232 g         4632 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt tt
//
LOCUS       DAYYJC010000059        15951 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000059 DAYYJC010000000
VERSION     DAYYJC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.059"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..809)
                     /locus_tag="ACHA9N_001816"
     CDS             complement(387..809)
                     /locus_tag="ACHA9N_001816"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9085273.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACHA9N_001817"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="ACHA9N_001817"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9085274.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="ACHA9N_001818"
     CDS             complement(2252..3154)
                     /locus_tag="ACHA9N_001818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9085275.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACHA9N_001819"
     CDS             complement(3168..4724)
                     /gene="atpA"
                     /locus_tag="ACHA9N_001819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9085276.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="ACHA9N_001820"
     CDS             complement(4747..5289)
                     /locus_tag="ACHA9N_001820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9085277.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACHA9N_001821"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="ACHA9N_001821"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9085278.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACHA9N_001822"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="ACHA9N_001822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9085279.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACHA9N_001823"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="ACHA9N_001823"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9085280.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACHA9N_001824"
     CDS             complement(7180..7644)
                     /gene="smpB"
                     /locus_tag="ACHA9N_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9085281.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACHA9N_001825"
     CDS             complement(7666..10023)
                     /gene="rnr"
                     /locus_tag="ACHA9N_001825"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9085282.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10026..10781)
                     /locus_tag="ACHA9N_001826"
     CDS             complement(10026..10781)
                     /locus_tag="ACHA9N_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9085283.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACHA9N_001827"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="ACHA9N_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9085284.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="ACHA9N_001828"
     CDS             complement(11238..11516)
                     /locus_tag="ACHA9N_001828"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085285.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="ACHA9N_001829"
     CDS             complement(11629..12597)
                     /locus_tag="ACHA9N_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085286.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="ACHA9N_001830"
     CDS             12880..13854
                     /locus_tag="ACHA9N_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM9085287.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="ACHA9N_001831"
     CDS             13859..14434
                     /locus_tag="ACHA9N_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9085288.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="ACHA9N_001832"
     CDS             complement(14501..15640)
                     /locus_tag="ACHA9N_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9085289.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5295 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DAYYJC010000060        15855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000060 DAYYJC010000000
VERSION     DAYYJC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.060"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..835
                     /locus_tag="ACHA9N_001833"
     CDS             83..835
                     /locus_tag="ACHA9N_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9085290.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            832..1746
                     /gene="pfkB"
                     /locus_tag="ACHA9N_001834"
     CDS             832..1746
                     /gene="pfkB"
                     /locus_tag="ACHA9N_001834"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9085291.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1764..3695
                     /locus_tag="ACHA9N_001835"
     CDS             1764..3695
                     /locus_tag="ACHA9N_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9085292.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3806..5365)
                     /locus_tag="ACHA9N_001836"
     CDS             complement(3806..5365)
                     /locus_tag="ACHA9N_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9085293.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5520..7754
                     /gene="pcrA"
                     /locus_tag="ACHA9N_001837"
     CDS             5520..7754
                     /gene="pcrA"
                     /locus_tag="ACHA9N_001837"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9085294.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7771..9807
                     /gene="ligA"
                     /locus_tag="ACHA9N_001838"
     CDS             7771..9807
                     /gene="ligA"
                     /locus_tag="ACHA9N_001838"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9085295.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9913..10218
                     /gene="gatC"
                     /locus_tag="ACHA9N_001839"
     CDS             9913..10218
                     /gene="gatC"
                     /locus_tag="ACHA9N_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9085296.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10218..11690
                     /gene="gatA"
                     /locus_tag="ACHA9N_001840"
     CDS             10218..11690
                     /gene="gatA"
                     /locus_tag="ACHA9N_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9085297.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11687..13117
                     /gene="gatB"
                     /locus_tag="ACHA9N_001841"
     CDS             11687..13117
                     /gene="gatB"
                     /locus_tag="ACHA9N_001841"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9085298.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13127..14182
                     /locus_tag="ACHA9N_001842"
     CDS             13127..14182
                     /locus_tag="ACHA9N_001842"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9085299.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14293..15666
                     /gene="rlmD"
                     /locus_tag="ACHA9N_001843"
     CDS             14293..15666
                     /gene="rlmD"
                     /locus_tag="ACHA9N_001843"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9085300.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15669..15797
                     /locus_tag="ACHA9N_001844"
     CDS             15669..15797
                     /locus_tag="ACHA9N_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9085301.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5392 a         2903 c         3481 g         4079 t
ORIGIN      
        1 aatttttatg aacgtttttg actgatttat attgctagta gaaaaaaaat atgctattat
       61 ttgtattgag gtgatcgtga aaatgcttac agaggaacgc agacaaaaaa tcctagactt
      121 gcttgaacag cagaaaatcg tgaaatcaca agatcttgta aaccttttga atgcttctga
      181 atcaacgata agacgtgatt tacaagagct ggagaatagt gggtttctag aaagagttca
      241 tggcggagct aagaaagagc aattattgaa ttttgagcag aatatgagtg aaaaatcact
      301 caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca gctgaaacca ttaatgacga
      361 agatgtcatt tatttagacg ctggctctac tacgttagaa atgattcctt ttctgcaagg
      421 aaaacagatc acagtagtga caaattccgt tcaacatgcg gcaaatttag ttgatttgga
      481 gcttgctacc attattttag gtggaacgat aaagctggca actaatgctg ttttaggatc
      541 taatgcgata gaacaactaa aaaattttca ttttaataaa gcatttatgg gaatgaacgg
      601 tgcccatctt gaacaaggat tcacgactcc ggatccagaa gaggcagctg ttaaacgttt
      661 agccatgaaa aattccgaag agacatttgt attgattgat cataccaaat tcaaccagtt
      721 gtccttcacc tctgttgcac ctttggaagc agccacgatc ataaccgagc gttgtccatt
      781 agaattcgtt aatgaattcc gtgaaaagac aacaattaag gaggcaatac aatgatttac
      841 acagtaacac ttaatccatc aattgattat atcgtacgcg tagatgggtt aaaactaggc
      901 gatttgaacc gaatgaagga agacttcaag ttacctggag gaaaagggat caatgtttcc
      961 cgtattttga aacggatcga ttctcaatct acagcactag ggtttttagg agggttcact
     1021 ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc agtctttgtt cacaaccgta
     1081 aaagaagata caagaatcaa tatcaaacta aaatcagaca ctgaaactga gatcaatggc
     1141 ttaggcccat ctatttcaga tgaagaaatc gatgagttga agcaagcatt aggaacagta
     1201 ggacaaggag atatcgtcgt attgtcagga agcgcgccag ctagcctgcg aaaaggattt
     1261 tatcaagaac tgattgatat cattcgagaa aaaggtgcag atttcgtgat tgatacaaca
     1321 ggtgatgacc taaaaaatgc acttgttaag aaaccgttgt tgatcaaacc aaacaatcac
     1381 gaattagctg agctttacaa cacaaccttc caatctgtag aagaaatcat tccgtatggg
     1441 aaaaaattac tagctgaagg ggcacagaat gtgctgatct caatggctgg agacggtgct
     1501 ttgctcttca cggaacaagg aacttataaa acgaatgtat tagttcgccc attgaaaaat
     1561 tcagtaggtg caggggattc aatgattgct ggctttattg gggcttacag ccgtacgaaa
     1621 gatccaatcg aagcctttaa atggggagtt gcttgcggaa gcgcaacagc tttttctgat
     1681 gacttggcag ctcgcgaatt gattgatgag ttgctgccag aagttgaaat cacaaaaatt
     1741 gattagtttc aaggaggata atcatgaata tcaaggattt aatggtcaaa gaagcgatga
     1801 tcatggattt gcaagcgact gataaaaaag gcgcaatcga tgaaatggtc caaaaaatgt
     1861 ttgatgccgg ccggatttcc gatatcgaaa catataaaga aggaattcta gcccgcgaag
     1921 cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc tcacgcgaaa aacagtgcag
     1981 ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg agtagattat gaagccttag
     2041 acggacagcc aacttatctg tttttcatga tcgcagctcc agaaggcgca aacgacacac
     2101 atttgcaagc attagcagca ctatctcgat tgctgattga tgcagatttc gtaggaaaat
     2161 taaaagaagc aaatacacca gaagaagtcc aagacttgtt ccaaacagct gaagagcaaa
     2221 aagaagcaga agaaaaagca gaacaagaaa cacaagctgc agaagcttct gctcatccag
     2281 aaagaaaatt cgttgttgcc gttacagctt gtccaacagg gatcgctcac acttacatgg
     2341 cagaagatgc tttgaagaaa aaagcgaaag aaatgggtgt ggagatcaaa gtcgaaacga
     2401 acggttcaga aggaatcaaa aaccgattga ctgctgaaga catcgcccgt gcagaaggtg
     2461 taatcgttgc agcagacaaa aaagtagaga tgaatcgctt tgacggaaaa gaactgatca
     2521 atcgtcctgt cagtgatggt atccgtaaac cagaagagtt gatcaatcta gcccttagtg
     2581 gtactgcacc agtattccat ggtgatggaa aagaaagcag ttcagaagaa ggaaatgcgg
     2641 atggtactat cggacaaaga atttacaaag atttgatgaa cggcgtatca cacatgttac
     2701 ctttcgttat tggtggagga atcgctattg cgctttcttt catgatcgac caatttattg
     2761 gcgtgccaca tgatcaatta tcaaatctag ggaattacaa tcaagctgct agctggttca
     2821 accaaatcgg acaagctgca tttggtttca tgctgccggt attagcagga tttattgctt
     2881 caagtatcgg tgaccgccct ggtttgatcg ttggatttgc tgctggtgca ttagctaata
     2941 caggcggtgc aggattctta ggtgcactga ttggtggatt cttagctggt tatgtcatcg
     3001 tatttttacg taaattattc aagaacttac caaaatcatt ggatggaatt aaaacgatcc
     3061 tcttttatcc agtgtttggc ttactgatta ctggtttctt gatgttgctg gtcaatgtcc
     3121 ctatgaaagc aatcaatgat gcattgaacg gtttcttgac aagtatgagt ggttcgaatg
     3181 ctgctttact tggtgcatta ttagctggaa tgatggcggc agatttaggc ggtccaatca
     3241 acaaagccgc ttatgtgttt ggtacagcaa ctttagctac gactgttgca actggcggaa
     3301 gtgtcgtgat ggcttctgtt atggctggag gaatggtacc tccattggca attttcgttg
     3361 ctacacgttt gtttaagaat aaattcagca aaacagatca agatgctggt ttgacgaaca
     3421 tcgtgatggg actatcgttt gtcacggaag gatcgattcc atttgcagca gctgacccga
     3481 ttcgtgccat tccaagtttt attattggtt ctgctttaac tggcggatta gtcggagctt
     3541 tcggaatcaa attactggct cctcacggcg ggattttcgt tgtattctta ctgagtcacc
     3601 cggtcatgta tttggtgttt attgctatcg gagcagtcgt atctggtgtg atttatggtt
     3661 tattgagaaa agctcctgca gtagcagaag catagaaata aaaaagaaga ggctgggttt
     3721 tcccagcctc ttcttttttt atagaatcat atagaatcaa gtagtgtaga tgaatagttg
     3781 ttgtaaaata gtttttccat gttcgttaaa atgattgctt caaatttgtg atagaagatt
     3841 tagcctcgat ttcctgcttg attcttggca agtttgcttc atcagtcaag ataaccaaaa
     3901 tatctcctac attcatgatc gtatcaccgt gagtcaagat ttcctgattt cctctgcgta
     3961 tagagattag cagcatattt ttaggccaat tgaagtctcg taccattaat ccgtctaaag
     4021 aactctctgc cgttactgga aaatcaaaag ttgtccttgc accttttagt gaggcggtct
     4081 gagtatttcc aagtagacgt tccaataatg attcgtaaat tggattaccg ccgagaaggt
     4141 cattgacgat ataagcaaca agagcagcca atcctaaagg catcagatga ttaagagagc
     4201 caaccatttc tgtcactaaa ataattgctg tcagcggtgc ttttccaatc gctgtgaaat
     4261 aaccagccat ggcaataacg acaaaatcct tgatgtagat tggatccatc ccgaaatcaa
     4321 ccataatgtt tccataaaag ctaccgatta aagcccccaa tgtcaatatc ggaaggaaaa
     4381 tcccccctgg aagaccccca ccgtaagaaa tcatggaaaa aacgaatcga agaacaaaca
     4441 aaccgataat cataatgatt ggcaaggaag catgtccaaa agctaaaacg atttgattgc
     4501 ctccgcctag agtactaggc cataaataac caattggcaa aatcagcaag aaaggaacga
     4561 acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa gccagcaaga
     4621 cattctgata aataaaacct aagatgccta gaagtactcc taagatcact aaggaaccat
     4681 aatatttcaa cggcaaagaa ggaaggtcac ctagatataa aacaggtctc aagccgaaaa
     4741 agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc caaacaagtg
     4801 gagaaaagga gtgatggatt tcttctaaaa caaagagtaa tcctgcgata ggagcattga
     4861 atgctgctgc aagtccgcta cttgcaccgc tagaaattaa aatcttttct tctgaagatg
     4921 gactccgaaa caattgtcca gctccttgtc caatggatgc gccaagctgt atagaaggtc
     4981 cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg aactttttcc
     5041 ataaaacaga gaaccagttc attgataatt ccccgcgaag ctgtccctct acttgtggaa
     5101 tcccgcttcc cttgatatcc ggttcatttt tgaccagata acccacgaaa aatgctgcaa
     5161 ggatgcagca aatagctagg ccaattaagt aaatcggctg ttcccgactc atttcataga
     5221 tacttcgaac taaatcgctt agcttttcga tagataatcg aaacaggcta acgacgattc
     5281 ctgtcagcag tccaacaaat attcctttta gaataaaata tatctgagtc ttatctaatt
     5341 tcttgatttt tccttttttt tccattcaaa cgcctcgctt tcttttaatt tagcgcgaaa
     5401 tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa ctagctagtt
     5461 taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg atgttgttaa
     5521 tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa gctgttcttc
     5581 acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg agagtgctga
     5641 cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac atcctagcga
     5701 ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg attctcgcat
     5761 ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt attttaagaa
     5821 gagatgttga ttttatcggg tataaccgga actttacaat catcgattct tctgaacagc
     5881 tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa tatgatcctc
     5941 gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct caggattttg
     6001 caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct gcttaccaaa
     6061 aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat acgattcgat
     6121 tatttgaaga acatcctgat tcactcacgt attatcaaaa caaattccat tatattcatg
     6181 tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta ttggcaggta
     6241 gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc tggcgcggtg
     6301 cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca gtaattttgc
     6361 tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa gtcattgaaa
     6421 acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga aacaagatca
     6481 cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagac cggatgcaag
     6541 aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac cggacgaacg
     6601 cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat aaaatggttg
     6661 gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat ttgaatgtgc
     6721 tggtaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc aaacgaggca
     6781 tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa tggccactgc
     6841 ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga cagcaattag
     6901 gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg acagtgacag
     6961 aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa atccaaaata
     7021 cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc acacaagaat
     7081 tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa gaaaaactaa
     7141 cggtcttctt aaatgatctg gcacttgttt ccgacattga caatttagaa gaagatgctt
     7201 cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaatttcca gttgtattcc
     7261 tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa gaaagcgaac
     7321 tggaggaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa gcgttgtatt
     7381 taacaaatgc tttctccaga acattatatg gacggacgca atataaccga ccttctcgtt
     7441 ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct acgcctaaaa
     7501 aaacgcctgt attcgctaaa aaaacagcat atagctataa acagccagag acagcggttg
     7561 ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga gataaagtca
     7621 aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg aaggatctag
     7681 aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc tttgcgccaa
     7741 ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat tgacgaagct
     7801 gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga atactacgta
     7861 gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga attagtagca
     7921 atcgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg agtaggcgga
     7981 aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag tttgaacgac
     8041 gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc agtgggacgt
     8101 gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt gcgttatgaa
     8161 gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga aaacatcact
     8221 gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat gaacatcgaa
     8281 gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca agacagagaa
     8341 gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc tttgcgtcag
     8401 ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac agtagctgat
     8461 ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga aaaaattgga
     8521 ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg gtcatacatt
     8581 gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat cgtcatcaaa
     8641 gtgaatgaat tctcgctaca agatcaactt ggttttacta tcaaagcacc gagatgggca
     8701 gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat cgaatggaca
     8761 gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg agtagcagga
     8821 acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa ggatatccgc
     8881 ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt ttcgcaagtc
     8941 gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca ttgtccagtt
     9001 tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat caatcctaaa
     9061 tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc gatgaatatc
     9121 gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt agcagatgtt
     9181 gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat aaaagaaaaa
     9241 tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt cgagcggttg
     9301 attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt agcagaacat
     9361 ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc gcttgatacg
     9421 atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga agtccatgaa
     9481 ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct tcgaaatgcc
     9541 caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac tggtaaacta
     9601 acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg gaaagtgact
     9661 ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg cagcaagctg
     9721 accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc agatgcattg
     9781 gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat ttcaataaac
     9841 aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac tcgtttagaa
     9901 agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa attagcaaaa
     9961 cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa aatcatcgat
    10021 atggttgaaa tgctggaaga agtagatacg gaaggtgttc catttacgtc gaatgtggtt
    10081 gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg cgatgaatta
    10141 ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat catcgataat
    10201 ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa ctgcatcata
    10261 tgcttgtatc gaaagaaatc actgtacaag atttgacaaa aacaacattt gaacgaatca
    10321 aagaaacaga gcctgaggtc gaggcattca ttacattgaa cgaagaaaaa gcattggaac
    10381 aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct ggtattccaa
    10441 ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca tctaagatcc
    10501 tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat caagcagata
    10561 tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt acagaaactt
    10621 cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt ggctcttctg
    10681 gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt agtgatactg
    10741 ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa cctacttatg
    10801 gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa atcggaccaa
    10861 tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt tatgacgaaa
    10921 aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg acaggcgaca
    10981 tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg gcccctggcg
    11041 taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct gttgtggaag
    11101 aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt gcctcatcag
    11161 aagcaagttc caatttgcaa cgattcgatg gtatccgcta cggttatcgc tctgaaaatg
    11221 tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt gaagaagtaa
    11281 aacgccgtat catgttaggt acattctcct taagcgcggg ttattatgac gcttacttca
    11341 aaaaagcagg acaagtccgt acattgatca aacgagattt tgaaaaagtc tttgcagatt
    11401 atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt gagaacatta
    11461 acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac ttggctggac
    11521 ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga ttgcagatca
    11581 tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt gaacaagcaa
    11641 cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga attttgaaac
    11701 agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt tctctccagc
    11761 gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact ggggttatcc
    11821 aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag cagctttggc
    11881 attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact tttacccaga
    11941 taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg acggatggat
    12001 cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc atttagaaga
    12061 agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt tgaaccgaca
    12121 aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag aagaagcata
    12181 cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg atgtgaaaat
    12241 ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg gacaagaaga
    12301 gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga aaaaaggttt
    12361 agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa tccaacaaga
    12421 aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca aagaagggtc
    12481 tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg atgacgaatg
    12541 gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg aacgctatat
    12601 ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa aagaaatgtc
    12661 tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat ctaactggtt
    12721 gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg agacaaaatt
    12781 gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga tcagctcaaa
    12841 aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc aagaagtagt
    12901 tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa tcattaatga
    12961 aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag atcgtgcggt
    13021 tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc ctggtgtcgt
    13081 gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga aaaaagcacg
    13141 cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg cggatatcct
    13201 acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac cagaaccaga
    13261 ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag tagtggcagc
    13321 tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg aacaccgtcc
    13381 taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat taaaaattcc
    13441 tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg taaaaatgga
    13501 catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct atttgacaga
    13561 attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg cttatcttgc
    13621 gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga agtatgatga
    13681 aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact ctgttggagg
    13741 attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt taatcattgt
    13801 gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga atggcggtcg
    13861 acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg ttgaaacaga
    13921 agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg gggaagcacc
    13981 gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg atcagattcc
    14041 tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca aagaattcgt
    14101 aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa tagcagacga
    14161 aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg tcagtgccgg
    14221 ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa aataggctta
    14281 aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata tacagtcgaa
    14341 atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta tccattattt
    14401 atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc aggcaataag
    14461 tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca gcctgtagaa
    14521 aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta tgaacaacag
    14581 cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa gatgccagaa
    14641 gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa taaagcacag
    14701 atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa gaatagccat
    14761 gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca agcaatcaaa
    14821 gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga aaaaaatgaa
    14881 ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat gatggtcgtt
    14941 ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc tattcatgaa
    15001 gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac aaatgtgatt
    15061 ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca gctattagga
    15121 aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca agcagagtat
    15181 ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt gatcgacgct
    15241 tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa ggtgtatggg
    15301 atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa taataaaatc
    15361 gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg gcaagaaaaa
    15421 gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga tcatcatttt
    15481 attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg taatccggca
    15541 acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa gaaagttcag
    15601 cctgtagatc ttttcccgca aacccatcat atcgaattag tcgcttcatt cgttaaagga
    15661 gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa caaggtggag
    15721 gccgatccaa acaaatggaa acacctaaga atgatcgtaa aaacatgaga aaaggcccta
    15781 aaatctttaa taaatagacg tgctaaagaa agactaagaa gtgttaactt ttgttattgt
    15841 ttcttagtct tttta
//
LOCUS       DAYYJC010000061        15707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000061 DAYYJC010000000
VERSION     DAYYJC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.061"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..220
                     /locus_tag="ACHA9N_001845"
     CDS             <1..220
                     /locus_tag="ACHA9N_001845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085302.1"
                     /translation="CLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKC
                     LNVFLNAKSMQKILYFSTFLSLTKIKKR"
     gene            complement(266..632)
                     /gene="ssrA"
                     /locus_tag="ACHA9N_001846"
     tmRNA           complement(266..632)
                     /gene="ssrA"
                     /locus_tag="ACHA9N_001846"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(803..1576)
                     /locus_tag="ACHA9N_001847"
     CDS             complement(803..1576)
                     /locus_tag="ACHA9N_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9085303.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1720..2070
                     /locus_tag="ACHA9N_001848"
     CDS             1720..2070
                     /locus_tag="ACHA9N_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9085304.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2051..2767
                     /locus_tag="ACHA9N_001849"
     CDS             2051..2767
                     /locus_tag="ACHA9N_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9085305.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2767..4215
                     /locus_tag="ACHA9N_001850"
     CDS             2767..4215
                     /locus_tag="ACHA9N_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085306.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4285..4794)
                     /locus_tag="ACHA9N_001851"
     CDS             complement(4285..4794)
                     /locus_tag="ACHA9N_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085307.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4784..5509)
                     /locus_tag="ACHA9N_001852"
     CDS             complement(4784..5509)
                     /locus_tag="ACHA9N_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085308.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5732..7852)
                     /locus_tag="ACHA9N_001853"
     CDS             complement(5732..7852)
                     /locus_tag="ACHA9N_001853"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9085309.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8082..9260
                     /locus_tag="ACHA9N_001854"
     CDS             8082..9260
                     /locus_tag="ACHA9N_001854"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9085310.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9276..10034
                     /gene="aroD"
                     /locus_tag="ACHA9N_001855"
     CDS             9276..10034
                     /gene="aroD"
                     /locus_tag="ACHA9N_001855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9085311.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10057..10542
                     /gene="ybaK"
                     /locus_tag="ACHA9N_001856"
     CDS             10057..10542
                     /gene="ybaK"
                     /locus_tag="ACHA9N_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9085312.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10613..11866)
                     /locus_tag="ACHA9N_001857"
     CDS             complement(10613..11866)
                     /locus_tag="ACHA9N_001857"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9085313.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11984..13333
                     /gene="gdhA"
                     /locus_tag="ACHA9N_001858"
     CDS             11984..13333
                     /gene="gdhA"
                     /locus_tag="ACHA9N_001858"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9085314.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13445..14791
                     /locus_tag="ACHA9N_001859"
     CDS             13445..14791
                     /locus_tag="ACHA9N_001859"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9085315.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15099..15485)
                     /locus_tag="ACHA9N_001860"
     CDS             complement(15099..15485)
                     /locus_tag="ACHA9N_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9085316.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15534..>15707)
                     /locus_tag="ACHA9N_001861"
     CDS             complement(15534..>15707)
                     /locus_tag="ACHA9N_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9085317.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5128 a         2812 c         3160 g         4607 t
ORIGIN      
        1 atgcttaaac cataattgtg tttttccttc catcagcata aaagttcacc tcaaaaagag
       61 tgtacgaaca aaagttcttt ttgtaaacct taaaatagat tttcctgaat acgtattggg
      121 gatttctaaa tgtttgaatg tttttttgaa tgcaaaatca atgcaaaaaa ttttgtattt
      181 ctctactttt ctctccttga ccaaaataaa aaaacgttga tttaacaacg tttttcatac
      241 atgtttatct gtctcaacca tacatatatg gagacggcgg gagtcgaacc cgcgtccaaa
      301 cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc actttacagc
      361 ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca acttacagac
      421 ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat gggcgatgcc
      481 gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt ttcgttttta
      541 gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg caattcaagc
      601 tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta cgtgtatttt
      661 tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg aatcatatcc
      721 cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt cttaagaaaa
      781 gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg tggacaattc
      841 cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg tcataggcgt
      901 tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc ggaccatcgc
      961 caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt ttgacacctg
     1021 ccccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc cgataaaaag
     1081 ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag atcagcaaca
     1141 tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct gaatgaatga
     1201 agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc tgatcgttat
     1261 agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga cattcttctg
     1321 aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa ccattcatgg
     1381 tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct tctgtacgtc
     1441 ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct tttgccactt
     1501 cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag aaagtgattg
     1561 cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat cttaacaaag
     1621 aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc agtcaacaat
     1681 ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt cagtattggt
     1741 atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg aaatctgcct
     1801 ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg gctggctcaa
     1861 aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat cggctattat
     1921 ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct agtatttcca
     1981 attatgccta tttttattat gacagaaaag aaattacaga aatatgacct ggaaactgga
     2041 gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg aaatgatgca
     2101 gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa aagaagggct
     2161 cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc tgaatttgcc
     2221 cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta ctgtgccgat
     2281 tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag agatgggggc
     2341 agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa tgaaagcttt
     2401 gcatcgccgt agtgagttgg cagaagcaag agaagaagca gcagtttcag atgatcattt
     2461 tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt atctggacaa
     2521 ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt tagcaaaaaa
     2581 accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt atcagtactt
     2641 tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa tcgaaaaggt
     2701 cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg attctggtgt
     2761 tgcctaatgc gttatttgta tcagcaactc ttagcttttt gcggaatggt cgcgttgatc
     2821 ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga agaaaacaat
     2881 taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac ctatgccgat
     2941 acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct gacagagcaa
     3001 gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg tgtcatttat
     3061 ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa aaatctgagg
     3121 aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc acaggcgacc
     3181 tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact tgtagttagt
     3241 caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct gttcaaagga
     3301 tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc attccaagtt
     3361 aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa ttttgatgta
     3421 cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt caataaaatg
     3481 acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg tcgaagacaa
     3541 tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa cggacttctg
     3601 gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat taaattgatg
     3661 aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta tgaaaaaatc
     3721 cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac attgaaaaat
     3781 attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac attgaaagcc
     3841 gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat ggtcaatatt
     3901 attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt agaaaaaggc
     3961 tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga acaacaaatg
     4021 aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac aaaatatggt
     4081 gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa aggaaaatta
     4141 gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc tgacgtagaa
     4201 atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt ctataccgct
     4261 tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa tataaggact
     4321 ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt catgagctaa
     4381 ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat caaatgtatc
     4441 ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca gctttacttt
     4501 ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga tatcaagttt
     4561 ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca acaggacatg
     4621 gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg caaaattatc
     4681 cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct gtttttcgag
     4741 aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt tcattacgtc
     4801 caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga ctaagaatga
     4861 gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata tctgtgaaaa
     4921 aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga attgtaagtc
     4981 cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt aagacaagaa
     5041 aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc cacaataaaa
     5101 ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa aggaataatt
     5161 tggttttaga aagaccagtc aggcgccaat tgatcaattc ctgcttgttt tgcctgactg
     5221 aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg aatatgcctt
     5281 ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat gcagaagcgc
     5341 caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa tggtaaagag
     5401 tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag agtattgtct
     5461 gctttttgta ataaattaat cgagcagtag catagcggat tattttcata cttttcacct
     5521 ctataaaaag tatagaaaat gaatatgaat aaagtatgaa attcctttag tgaaatcacg
     5581 aattaaaacc agaaaatatt ttgtaaaaag caagaggttg tgaagaagct gccctattct
     5641 attaccgtag atgcaagctt ttgaacactg tttattcgaa aagaggagca gcgacaataa
     5701 ttttgtcgct gctcctttta aatttatttt gtcattcgga agaacttgtg gtcgttccgt
     5761 gaagttcttg gtacgtttgc gttttataaa gatcaacagt tgattgtccg ttgtgttcac
     5821 taaagacact agttgacttg tcaccaagag atttttcagc ttcttttaat ttctctaagc
     5881 cattcttgta gtcataatca ctggtatcaa ttgggtctaa tccactattc gtatagaacc
     5941 gaagaagatc accattattg atttggtcag aaacagctaa ctgtttatca actttttctt
     6001 tcagtgaatc aatttcattc tgtacttcct cagtaggttc agtaatagct tcaccagttt
     6061 cgttactata gatcgtacca ccgtagtaag tatattttgg tgtaacaaaa tcaccatcac
     6121 ggaaagaaac aagctgttca tgatctttcg agaatagatc ttgtcctaac tgaatataat
     6181 tttttgtatc tacacctagt aagtgaagta atgtaggcaa ggcatcaact tgtccgccgt
     6241 aagtgtgatt gattccgccc tgtgtttgtc cagggatatg gatcatataa gggacacgct
     6301 gcatttgtgc attgtcgtag tttgtccaag tagaacttgt ttttccaact aattcagcta
     6361 gatttttatt tcttgaattc gagacaccgt aatggtcacc gtaaaggacg atgactgaat
     6421 tatcatacaa gccgctttct ttcaaataat tgaagaattc ttgtacggcg gtatctaaat
     6481 aattggctgt cgcaaagtaa ccgttgatcg tttcatcatt tgtattggct aacgggaatc
     6541 ctgcttcgtc attggtgaat tttgaataag gataatggtt agatacagca atgaattttg
     6601 aataaaatgg ttgttgcaga cgttccaagt attgtacgga ttgttggaaa aatggtttat
     6661 catgcaaccc gtattggaac gaattgtcat cattaacatc ataataagaa gaatcaaaga
     6721 aataatcata tcctaaattc ttgtatgtct cgtttcgatt ccagaatgta ccagcgttcc
     6781 catggaatac cgcactagtg taaccttgtg tttgacttaa aatatcaggt gcagcttgga
     6841 aagtattttt tccgcctaat tgtgtgaaca aggagccttg attcaagcca aacaatgaat
     6901 tttccatcag cgtttccgca tcgcttgttt ttcctgcttt tacttgatgg aaaaagttgt
     6961 caaaactgaa cgtgctgttg ctgtggtaaa gactattgat gaaaggtgta acttcatgct
     7021 ggagaccatt ctcatctgtc aatttataat cgatcaggaa ttgctggaag ctttctaaat
     7081 ggatataaat cacatttttt cctttagcga gcccgaacaa atcgtcattt ggtgctgcat
     7141 aatgttcttt tacataattt tctacttctt tcatatcatt gggacttgct tctgcccgca
     7201 cttggtttgt ctggtaagtt tgaacaccgt cataaaccat gaaagcattc aagccaagat
     7261 atttgaccaa ataatcacga gagaatgttc gagtcagcaa ttctggacga tcagcttccg
     7321 ccataaaaag atttcctgag aaaatcataa ctgctaatgt agagatcgca aaagccattc
     7381 ttgctcggat cgggcgaggg tccaacttga ttttttttgc aatcagcaga acagtcaaga
     7441 taataaaatc gatccaatag atcacatcat atgggcgaaa cagtcgaatg gcactttctc
     7501 ccagaccact cgctactttc ccagcaccta aaatcgtgtt gatcgtaatg aagtccgtga
     7561 attctcgata ataagtgaca ttcgaaaaaa caagcaatgt caaaagaaaa taaatgacca
     7621 tcatcgtgat ataagcaact ctagggcgtc tgatataaag agcaattcca agtaataaca
     7681 atgtagttgc aatagggttg ataaacaaga taaagtattg aatgccactt tcgattccga
     7741 gatggaaatc aactaaatag gcaaaaatcg ttttcaacca aagtaaaacg actagtaaag
     7801 aaaaaaatcc gagcctctta ttcaaaaagg caggtgtttt tatacatttc aagataatct
     7861 tcctttctgc acttcttaat cacagaagtg tcatgaaacc atgttacaca gctcacttat
     7921 tctcatttat tttactagta tgacaatata gtgtcaattg aaaccagaag tttatgctgt
     7981 ttcattaagg ttttctttaa gcttcatgaa aaaatgagca gtaagacaag tcctatgttt
     8041 tttgttatac ttgggaagag aaagctaaag caggtgaagg aatgaaagta aaagtaaaaa
     8101 aaagagcaag caatcgattg aaaaaaggat atccactgat ccaaaaagaa gaccttgctc
     8161 atccccaaaa agaaaccacg caatgggtgg aattagttga ttcgcaaaac caatttatcg
     8221 caaaaggata tctaggaaag cagaataagg gaatcggttg ggtattgagc cagcagaaca
     8281 tcccatttga tcaatctttt tttgaagaat tttttgtgaa gtcaaaagaa aaaagaactt
     8341 tctttttttc agatgattct actaccgctt ttcgtatatt caatggagaa ggagacggaa
     8401 tcggcggtct gattgttgat tattatgcag gttatgctgt tttttcttgg tataacgaaa
     8461 cgttatatgc acatcgtgaa ttattgattc aagcttttcg tgaaatctac ccggaaatcg
     8521 taggaggcta tgagaaaatt cgatttgaat cagtaaatct gcctgagagc cagttcattt
     8581 acggaaaaga ggcaccggaa cccttgatcg taaaagaaaa cggagtaaaa tttgccgttt
     8641 atttaaatga aggattaatg actgggatct ttttagacca aaaagaagta cggggatcat
     8701 tagcagaagg tttcgcttct gggaaaacag ttttgaacat gtttagttat accggcgcct
     8761 tttctgttgc tgccgcaatg ggaggggcaa tcgaaacaac aagtgtcgat ctagcaaaac
     8821 gcagtttaaa aaaaacacag gaacagtttg aagtgaatgg tttggatgtc gatcagcaaa
     8881 aaatcattgt tatggacgta ttcgagtatt tcaaatacgc caaaagaaaa caatttttat
     8941 atgacatgat tgtattggat ccaccaagtt ttgccagaaa taagaaaaaa gtctttcgcg
     9001 tagcaaaaga ttatggggaa ctggtcaagg atagtctgga tattttagca gaagacggga
     9061 tacttattgc gtctacaaat gctgcgaatg tctcgataga acaattccaa acaatgattg
     9121 aagaggaatt ccatgccaaa aatgtttctt ttgaacaact tgctttatat cgtctgccaa
     9181 aagatttcca gacgacggat acctttttag aaggcaatta tctgaaagtg tttgtttatc
     9241 aaattaaaaa taaccattag aaggagcgat gcagaatggt ttgggtaaag ggattgaata
     9301 taggaagcgg tagaccaaag atttgtgcgc ctcttatcgg ggccgtccga gaggaagtat
     9361 tacgtgaagc agttcttgca cgtgaagcag gggccgattt agtcgaatgg cgagtcgatc
     9421 attatcgtga agttttttct tttgatgaag tagcagaaac attggttctt atccatgaga
     9481 tgcttgatgg actgccgtta ttatttacgt ttcgtacctt agctgaaggc ggagaacaag
     9541 acatatcgat ccgtcagtat cgcgaccttt atgaacatgt gatacacaca ggattagtag
     9601 atatggtcga tatcgagttg ttcaaagtag aaagtctagg aaaaagactg atagaggaaa
     9661 tcaagtcttt aaacattcct ttaattatca gtagtcatga ttttaaagaa acaccagcag
     9721 accctgtctt gctttaccga ttgaatgtta tggaacattt tggagccgat atcggaaaac
     9781 tggcagtaac tcccaataat gaacgagatg ttttgcggtt gatggagttg actagacggg
     9841 caaatgcttt tgtctccatg cctattatta cgatgtccat gggaaatctt ggtaaaattt
     9901 ctcgtctggc tggcggaacg acgggttccg ttatgacatt tggttcctta agcagcgatt
     9961 ctacttctgc tccaggacaa ttaccggtca aagcattgaa gcaaattatt caattattaa
    10021 atgaaccgtt gtagaacaag tttgaggtga tattttttgg caaagaaaaa aatagtaaaa
    10081 acgaatgcaa tccgtatggt cgaacaaaaa aagattccct atacagagca cgaatatgag
    10141 tgggacgaaa gccacctaag tgcgtcatcg gtcgctgaac agcttccaga aagccaatct
    10201 cgtatattta aaacactggt tgctgtgggc aatgtgacgg ggcctcttgt tgctgtgata
    10261 ccgggagaag ccgaattgaa tctaaaaaaa ttagcaaaag tttcagggaa taaaaaagta
    10321 gaaatgctcc atttaaaaga tttggaagca acgaccggat acatacgtgg tggctgctcg
    10381 cctataggta tgaaaaaact atttcctact tatttagatc aaattgcaga aagctatgaa
    10441 caaatcattg tctcggctgg cagaagaggg ctacagatgg agttagcacc tcaagatatc
    10501 tgtgcattaa cgagcggaca atttgcagat ataaaacaat aaatcatacg tataaaagaa
    10561 agaaggaggc cgggatgttg tttgtccagt ctccttcttt cttttttata cattatgctc
    10621 tttgcccggc attcattcct gctacatgcc ctgtagcgaa agccgctgtg atattaaagc
    10681 cgccagtata accattgaca tccagtaatt ctcccgcaaa atacagacca tcaatgattt
    10741 tactctccaa tgttttagga taaatttcct ttagagaaac ccctccgcca gttacaaatg
    10801 atttctcaat aggaaaagtt ttgttgattg atagcttaaa ctctttgcat agttgaacaa
    10861 aatcttgaat ttgtttttga gaggcttgtt gtcctgtcag atgatccatt tcaagccgct
    10921 caagaaagaa aactaacaat cgctcaggca atataccatg ccaagcattg accaagttct
    10981 ttttggttgc tttacttttt tctgtcaata tatgaataag ctcttgtttg gtttgtgtag
    11041 ggaaacaatc cagagaaagt gtcactggtt ctcccgtttt ctctaattct ttattgacaa
    11101 aacttgaaca acgcagtgca gccggaccag aaataccgaa atgggtgaaa agtaaatcca
    11161 tcgtatgttc agtcaataca cgaccttttt tatttaaaac gcgtaaagtg atgtcttgta
    11221 aagaaagccc ttgaagtgtt ttctcctgaa tatacggttc atccgatatc aagggggact
    11281 ctgtcgcata aagaggcgtc accgtgtgac ccactcgctt cacgatcttg tacccatctc
    11341 cagtagaacc agtagatgga taagtccgtc caccagttgt taagataatg gcaggagcat
    11401 ggaactcttc gaattccgtg cgtataccgt aaattttatg ttctttgtgg atcaatttct
    11461 ctactcgagt agagaataga agcgtcacat ctagttcttt cagacgattg agcaatgctt
    11521 cgataatcgt ttttgattta tttgttacag gaaacatccg tccatgatcc tcttctttta
    11581 aatgtacgcc ttgtgattca aagaagttca tgatatcgaa attattccat tgcgcaaacg
    11641 tactatataa aaactttcca tttccaggaa tatgtgcaat caaatcatca acaggacgat
    11701 tgttcgtaac attgcaccga ccaccacctg tcatcagtag tttttttcct gcttttttgt
    11761 ttttttcgat caagagtacg cgtgcgcctt gttcagctgc actgatcgct gccatcatcc
    11821 cgcttgttcc agcgccgact acgatcacat catacagttt ttccattccc tcacctcaag
    11881 gaaagtgtag catatctgaa gaaagaaaac agaaaaaaaa tgttttgagc caaaagagaa
    11941 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
    12001 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
    12061 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
    12121 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
    12181 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
    12241 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
    12301 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
    12361 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
    12421 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
    12481 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
    12541 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
    12601 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
    12661 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
    12721 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
    12781 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
    12841 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
    12901 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
    12961 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
    13021 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
    13081 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
    13141 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
    13201 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
    13261 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
    13321 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
    13381 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
    13441 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
    13501 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
    13561 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
    13621 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
    13681 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
    13741 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
    13801 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
    13861 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
    13921 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
    13981 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
    14041 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
    14101 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
    14161 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
    14221 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
    14281 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
    14341 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
    14401 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
    14461 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
    14521 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
    14581 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
    14641 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
    14701 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
    14761 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
    14821 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
    14881 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
    14941 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
    15001 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
    15061 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
    15121 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
    15181 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
    15241 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
    15301 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
    15361 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
    15421 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
    15481 ttcatgaaag atctcctttt ttatttttat tatacccgtg gtgctagtta attttaatat
    15541 cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa tagaacactg
    15601 taagccagta atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa
    15661 ttttgacaac ctaatttttc taaggaagaa aaaacagtct caactgt
//
LOCUS       DAYYJC010000062        15494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000062 DAYYJC010000000
VERSION     DAYYJC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.062"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..427
                     /locus_tag="ACHA9N_001862"
     CDS             77..427
                     /locus_tag="ACHA9N_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9085318.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            526..1056
                     /locus_tag="ACHA9N_001863"
     CDS             526..1056
                     /locus_tag="ACHA9N_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9085319.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1210..2361
                     /locus_tag="ACHA9N_001864"
     CDS             1210..2361
                     /locus_tag="ACHA9N_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9085320.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2470..4509)
                     /locus_tag="ACHA9N_001865"
     CDS             complement(2470..4509)
                     /locus_tag="ACHA9N_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9085321.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4567..5124)
                     /locus_tag="ACHA9N_001866"
                     /pseudo
     CDS             complement(4567..5124)
                     /locus_tag="ACHA9N_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(5272..5438)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(5663..5869)
                     /locus_tag="ACHA9N_001867"
     CDS             complement(5663..5869)
                     /locus_tag="ACHA9N_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085322.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5885..6499)
                     /locus_tag="ACHA9N_001868"
     CDS             complement(5885..6499)
                     /locus_tag="ACHA9N_001868"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9085323.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6646..7329)
                     /locus_tag="ACHA9N_001869"
     CDS             complement(6646..7329)
                     /locus_tag="ACHA9N_001869"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9085324.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7438..7896)
                     /locus_tag="ACHA9N_001870"
     CDS             complement(7438..7896)
                     /locus_tag="ACHA9N_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9085325.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7980..9743)
                     /locus_tag="ACHA9N_001871"
     CDS             complement(7980..9743)
                     /locus_tag="ACHA9N_001871"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9085326.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9964..11424)
                     /locus_tag="ACHA9N_001872"
     CDS             complement(9964..11424)
                     /locus_tag="ACHA9N_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085327.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11632..12558)
                     /gene="glsA"
                     /locus_tag="ACHA9N_001873"
     CDS             complement(11632..12558)
                     /gene="glsA"
                     /locus_tag="ACHA9N_001873"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9085328.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12581..14008)
                     /locus_tag="ACHA9N_001874"
     CDS             complement(12581..14008)
                     /locus_tag="ACHA9N_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9085329.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14039..15253)
                     /locus_tag="ACHA9N_001875"
     CDS             complement(14039..15253)
                     /locus_tag="ACHA9N_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9085330.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4435 a         3094 c         2662 g         5303 t
ORIGIN      
        1 aataatcgct ttttcttctc tttttcttat atactataag tagtaaataa tttgaaaaaa
       61 agaaaggtga ctgattatgg cgcaaaatcg tatttttacg acaagttttg caagcgttta
      121 tcctatgtat gtagcaaaag cggagagaaa agatcgtata aaagaggaag tagatgaaat
      181 catttgttgg ctgacgggtt attcccaaac agaactagaa tatcaactac aaaaacaagt
      241 gactttcgaa caattttttg cggaagcccc tgtccccaat ccttcacgtt ccttgattac
      301 aggtgttgtt tgtggtgtac gagtagaaga catcgaggat aacttgatga aagaaattcg
      361 ttatttagat aaaatgatcg atgagctagc aaaaggcaaa aaaatggaaa agatcctcag
      421 aagttgattt ttccagagat attcttctcc ttcgctcatt ttgtaacatt taatttggta
      481 aaatgactag ttcacgttaa aataagtcaa aggaggtttt cttatatgtt tgagacgatt
      541 aaacaaaata ttcaaaaata cgccttatta agagctgctg tttatattat ttctgggatt
      601 gcgattgtgc taaacccttc tgctgttttt cattttatcg gttatctgat caccgcctat
      661 cttgttttgc ttgggttgtt gaatcttttt gaggcttacc gttttaaaag gaaaacaggt
      721 tcttggagta tcggtctgtt cagtggtctt tttttcctga tcttagctct gatcgtctat
      781 gcttttgcac ctgcaatcgt ttctatcctg ccgattcttc ttggattagc tgttatcatc
      841 aacagcttgt tccagttgtt cttcagcatg aatacaaaag ctaaaagttg gagtgtttat
      901 agtagtattc tgttgttgat tggcggattc gtcttattgt tcaatccatt caaaagtttg
      961 atgcttctat tccaaatatt cggctttatt ttgattttca tgggcatcca ggagattatc
     1021 aatttcatca gaataagaaa aatgactata cgttaatcat ttttagttat taaaatagac
     1081 cgtataaaaa ccaacaatcc aaaaaaatct tttggattgt tggttttttt gtgagtatat
     1141 tttcctattt tttcaataat gtatgttatg ctaagttcgg gctatatttt atacaaagga
     1201 atgatgagaa tggaatttat tggtatttta tgcctgatat tgatttcaac aacgattggc
     1261 tcacatatct cgcggagatt aggtatccca gcagtgattg gacaattact tgttggtgtt
     1321 ttgctaggcc aagctggatt gaattgggtc aaacctgata ttctagtcca tgacttttcg
     1381 gagattggtg tgatcttatt aatgttttta gctggtctgg aaagtgattt atctcttttg
     1441 aaaaaatatt tccgacctgg tatgttcgtt gctttgcttg ggatcatctt cccggttgtt
     1501 tttggctggt tgacaggcaa agcattccaa gtttcgggaa atgaagcgat tttctttggg
     1561 attattcttg cggcaacttc tgtcagtatt tcagtagaag tattaaaaga actaaacgta
     1621 gtgaatacaa aagaaggatc aacaattttg ggtgcttctg tggtagatga cattttagtc
     1681 gttttagttc taagtttcag cctatctttt ctaacggggg aagcttctag caatgcttct
     1741 ttaccgcttg ttttgattga acagctaatt tattttgttt ttattttctt attagtcaaa
     1801 tggattgcgc ctttcctact ttcatttgca gaaaaagtgt acgcaaattc ttctattatc
     1861 atcgtatcgc tagttatttg tttaggaatg tcttacgtag cggatcttgt tgggcttagc
     1921 tctgtcattg gtgctttttt tgctgggata gctgtcagtc agacaaaggt taaagaagaa
     1981 gtataccaca atgtagaggc tttaggatat gcagtattta taccagtatt ttttgtcagt
     2041 gtaggattag aagttgattt ttctgacttt agtgaacaac tactctttat ttctatattg
     2101 acgcttgtag cgattttgac caaattgctt ggaggttatt ttggtgctca gattgccggt
     2161 ttttctcaac atagttcatt gatggtcggt gctggtatga tttcgcgtgg agaaatggct
     2221 ttgatcattt tacaaattgg acagcaaagt caattgatcg aaccacagta ctattcacct
     2281 ttagttatcg tgattttatt aagtacactt atttctccac ttgttctcaa atattttaca
     2341 aaaaaagttt atgaagtgtg agtaaatcaa aaagaagttg tgaaagagac atcgtgaaaa
     2401 ccgtttattt gaaaaaagga ctgtgacgat aacgccacag tccttttact gtacttaaaa
     2461 agcaattttt tattgataat tttggttgag gtattgcaat agttccgatg ctcgtttggt
     2521 tgctgaatca tcatcttctt tattttccaa catgctgacc atcatataac cttggttatc
     2581 aagattgaaa gcaaacaaga aactgttctc ttgccccttt acatcctgtt tttctttgat
     2641 ttccgctgta ccagttttcg ctgccaacgg aatccctaaa gcagaaagag aatgtgctgt
     2701 accatttaca tcttgcacaa cttctctcag atctggcacg atcgtttgta ctgctgtttc
     2761 gctgatgaca ttcttcttat cttttgtctc tttatctgca atcaatttag gatagacaag
     2821 tgtgccattg ttggtaaaaa cagaatacat tgctgcttgc tggataggag taattagaag
     2881 ttcgccctgt ccatatccag tatcagctag caagatatct gagttaaagc tatcttcatt
     2941 agaaatttgt gctggattca tactaattgg caaatcaagg tcttcaccaa aaatgaattt
     3001 atctaagcct gtacgaaatt ttttctcacc cattttcaac gtttcttgtg ccgcatatat
     3061 attatcggaa tagatcaaag cagtttttaa gtctacttgt gatacatcac tactgacacg
     3121 cgttgtttta aaagatcccc aagaactgtc tttttgccat ttaagcccgt tgatcgtcaa
     3181 cacttcattt ggatcgatag tgccgttgtc gagaccgatt gctgctgtaa tcattttaaa
     3241 cgtagatcca ggagcataac ctgtcgcaaa tcggctgatg aatggatgtt caggattttc
     3301 ttcataagct ttgtaatctt cttgtgagat cccgtttgtc attttgtttg gatcatagct
     3361 tggagagcta gcaagcgcaa gaagatcacc ggtttttggc gttgtcgcaa cagttgatgc
     3421 agcttttcct cctagactgt caaaagctat tttttgtgcc ttcgcatcga ttgtcaattt
     3481 gatatctttg ccattttgaa cttcatgttc gatcagaact tttttctcta caccgtctgc
     3541 atctgtaatg cttaatttcc cacctgttgt cccacgaaga tccttatcaa aagccatctc
     3601 caaacctgag cgaccgattt ttccattact gcttaattct ggattcttat caatatcttc
     3661 tgctgtgata tctcccacgt aaccaatcag ctgagcagct gcttcaccca aaggataata
     3721 tctgccgtct acttcttgaa tggtagctcc agctggaagt tctggcgttg ctccatcaat
     3781 gattttcaat gggacaaagt aatcgggttg tacccagctt tgtgaaatcg cctgattgat
     3841 agcatcttct gttaagtcga aggaggaagc aatcgctttg atattggctg ttttttcgcc
     3901 tccatctcca agtttacttg gaacgactcc taattgtttt agtttgcctg ttgttgctaa
     3961 tggttcacca tttcgatcta taatattccc tcttgctgct tcttgtgtgg tcaggcttac
     4021 tttgtcattt ccttccattt ctggaaaaac caagttgggt tgccagttga tcgtggtttg
     4081 accatcattt ctgtctaatg ttcctttata agaaagatct ttcaattcac ctaatgaggt
     4141 attcatcttt gctttatatg aaaaactgta agtagaatcg tcttttttat ctacttttag
     4201 attcgatatc tgaagtcctt tgacatcagc agcaccgtat atattctggt atttatctaa
     4261 gatctctttt tcagataatg cacttttatt tgccgccttt tttgatgtca tttctgcagc
     4321 tttgttataa tctcccttgt tcaaagcttg gacaaattgc tcaactgtct tttctccagc
     4381 ttctactgct tgggtttctt gatagtgctg gtagtaaaaa taaccgccac ccgcagctgc
     4441 tattaagatc actgcgccgg caatataagc gcctgttcga tttttgccgt gcttgtcact
     4501 tcttttcatt ttttcctcct tctcttgctt gtcattcctt tatttcacta tttatacctg
     4561 tttattctaa catatgtctt tgaggaaatc cataccacaa tccagaagca cactttctgg
     4621 aaggttcgtt tggatactac caagtaattt cccaaccgct ttgggtagct tgaatattga
     4681 agttacgtct ttcagctgct gagagatagc tttgatcact tgctgctgcc ttctgatcct
     4741 cccgaagtct ccttctttgt cttcccgaaa tcgagcatac tggagtaaag tattaccatc
     4801 catcacttgc tctcctttgt ttatatatac gccatctaaa tctagagatt tttctgcatc
     4861 gatcttgact ccaccaggaa acagttcatt aacgcaatcg ataaaatctt gaaatgtgat
     4921 acttgcataa tagggagctt caaatttgaa attttccttt aatgtttgat ttaataaatc
     4981 aactccccca taagagtaag ctgcatttat cttattcatt ccgtaacctg gaatatcgac
     5041 atatgaatct ctcatgatag agatcagttt tgcttgttgt gttttctggt tgtattgtgc
     5101 aaccagaagt gaatccgagc gacctccacc aatcgatctt ttttcaccgc atctcctcct
     5161 cacacagcaa tccacttgtc aggtgaggag cgagtttgtc tcatcaaata agtcagtcac
     5221 aaaaatataa agaaaaggtc agaaaccttt tcgaacaaga attctgacct agcatcatct
     5281 catcgtccga gttttagcac tatacaacgt aaagaaaaca ttcttagcta ttggaaaaag
     5341 ccttaatcca acagtttctg ttttacccat tggcgacttt cgacgtttct gggcaacagc
     5401 ctatgtttgt ataggagcct cacctaacaa ggttattact atattctatt tacgagttca
     5461 tgatagactt ttcctaatga ttaggcaatg gaatttgaaa tgaaatagtg aaatttaaca
     5521 atctttaata ttttttagaa aaaaataaca aataagttga aagaatcccg ttgcaaattc
     5581 tttcagttgc accaggattc tttgataatt ttttagtttt atgtgttttt ttatatgttt
     5641 tttcactttt cggaataaat atttatttgt taaagtaatt aaacaatccg ctaatcgttt
     5701 gatctggcat ttctatttcc ttaccatcta aagtaacagt agtaacgaca aaatggtctt
     5761 gcaatggcgg aacttctaag tactgccctt gcagaaaatc attcactgtt tcatattctt
     5821 ttttcatttc ttttgaatct gtcgatggtt tatagcttat ttttaacata actagtgctc
     5881 ctttttattc gggacgtgca atcgtgaaaa tttctcctat tgctgcataa tcatttcctg
     5941 ccaaacggct tacagcattc aattttcttg gatcgatcct tcctgtctta tcatcataga
     6001 ctgtttcatt taaatgatat ttctgtactt ctagcaaaaa taaatcagct gttactttat
     6061 ttttattccg tatctcgaca tgttcatata aggatgtttc aaaacgaact tttgcttctg
     6121 tcaatgctgg tactgaaatg attactgaat cagttaaagt caagtcagtc aggtacagtt
     6181 cgctttcctc attaggcaga ttggctgctg ttttatttac ttcttctacg ttgtcctcat
     6241 caaggatatg gataacagct tctcttgtct caagaatatt ctttgctgtg tcttttactg
     6301 catcttgttt tctctggatc gccaaggata agatgggtcg attcgaactg acgatattga
     6361 agtaactgaa gggtgcgata ttcactatat cttttgctga tttagtcgtt actactgcta
     6421 ctggtctagg gatgactgaa ccaatcagca acttgtaatt ttcacgttct gtcaattctg
     6481 aaggactccg ttcaaacatg gtttcgcctt ctttctataa aattcgttaa aaagcagatc
     6541 ttgaagacaa aatgaaaagt aatgcttttt tcatcttctt agaatctgct ttaaactatc
     6601 tatcaatttt tcttttttgt caagaatctg tgccgtgcgt gttttttagt tatttgcttt
     6661 acttgcggct tcgacatatc ttttagtgat caattgcgga cgggtgataa tagatccaac
     6721 tacgactgaa aaagcaccta attcatatgc tttctctact tgttcaggag agtcagtatg
     6781 tccttccata attactggga tatcgacttg ttctatgatt tgattcaata aatcaaaatc
     6841 aggtgtctgc gtctttcgtg aatattctgt atagcctgct aaagtcgtag acaaaagatc
     6901 aaaaccatct tcttgcgctt gaagcgcttc ctcaaaagtc gaaatatctg ccatagctaa
     6961 ccgaccgtgt tcgtgaatca tttggacaag ttcagcagtt gttttttctg tcggacgttt
     7021 acgagaagtt gcatccagtg caatgatttc tgcttgcgtc tccaaaagtt cttccacttc
     7081 tttttctgtc gcagtaataa acacttctga cccatcgtaa tctcttttga taatccctat
     7141 taccggtaaa tcaacgattc gcttgatttc attgatatct tctgtcgtat ttgctcgaat
     7201 accaactgct ccgccttctt ttgcagccaa agccattctc cccataataa aagaactgtg
     7261 tagcggctcg ttttccaaag cttgacaaga aacgacgact ccgcctttga tttgttccag
     7321 tgtatccaca atatctcctc cagtaatgtt atataaaaat acagcttcat ataaaaatgt
     7381 aacccttttc tattcaggag acaataatga gctatttctt tcagcagacc tttcttacta
     7441 aaagaaaatt acttcaaatt cttcgtatac gaccagcttc tcttctgtaa attctatgtt
     7501 caacgcttct ttataagcat gtttaaaaaa ctcagcatgg actttccgcc aataagctaa
     7561 tgtccgatcc ccttctcctt ctttccaagc atgctctttc gtgacttcac taaaaggcaa
     7621 gatggtcaca tttatatttt tgatgataca aactggttca ttttttccat tcaggaccac
     7681 gtctaattga ccaacttttg gtaaaggctc attctcttgt ttatacagat cgtaaccact
     7741 tgatgtacca gtctttatct tttttacaac tagttcgctc aactcgtctg ccattttttc
     7801 tgtatcccca aatgaaaaga aacctaattt ttctgattga taactaggat atttttcttg
     7861 gaactttttg acatattctt ctacttgctg atacataaca tccacccctt tgctttttat
     7921 aatgaaaaaa ccatccagat actagtataa acaattatct ggatggtgag cataatgaat
     7981 tattgaatat tcaagacacg cagactatct tcaacgattt gttttggtgt taaatgataa
     8041 cgttcataaa gctgccctac aggaacgcta tccgtgaatt ctttctctgc accaaaattt
     8101 aatacgttca ttgaagaatc accataataa cgactgatat tttcgccaaa tcctcctgac
     8161 aaactaccat cttctaatgt gatgactaat tcgtgtgttt cttttaattt atctaatgtt
     8221 tctacgtcat tttctgtgat cgttcctgga ttgatcaacg ttgcttcgat tccttttttt
     8281 ctaaaagctt ttactgtttc ttttcctaat tcaagaaaac cgcctaaagc taaaattgct
     8341 acttttcctc cattatttac tgtttgatag gctggaatac tataatcatt tgcgatagct
     8401 gtaccatgtt ctacaccatg tgtaggaatt cggattgcta caggctgcgc tgtttgttcg
     8461 aacgcccaat ctagcataga gatcatatcc tcttttgttg ctggagcaag atatttgatg
     8521 ttcgggatac ttgataagta aggaatgtca aatgttcctt ggtgtgttcg gtcactagct
     8581 gagatcgttc cgccaccgac taaaataatc actggattct cgttgatcgc taaatcatgt
     8641 gataattgat catatgcccg ttgcaagaaa gtacttgatt ggaagacgac tggacgtact
     8701 ccctgagaag ccaaagcaga agcgaaagtg atagagtgct gttcagcgat ccccacgtca
     8761 aaataatgat ctggatgttt ttcttggaat gttttcagtc caaaggcgcc tggtatcgct
     8821 gcattgactg cggcaatagg agctgtactt gtttgcattt tcttatccaa ataatccatg
     8881 atgatcatat taaatgtttc tccagaagtt acttgctttg cctccccggt ggacagatca
     8941 aatggtacat gccaatgata tttcatttta ttatcgattg ccggctgata accgcgacct
     9001 ttttccgtat ggacgtgaag tacaatcgga tgatcaatat cttttacttc ttcaaataaa
     9061 cggatcaccg tttccagatc gtttccatta tccacgtaac gataatcaaa tcccattgct
     9121 ttaaataggt tattgctgct tttaccgttg gtttgacgaa gctcagctaa gtttttatat
     9181 aaactgccat gattctcatc aatagacatc tcattatcgt ttactaatac gattagattg
     9241 gattgtaaaa caccaccatt gttcaatcct tcaaaagcta agccaccact tagggagccg
     9301 tcaccgataa atgctaccac atttcctgat tctttcttca gatctctcgc tttagctaaa
     9361 cctgttgcta agctgataga tgtagatgtg tgccccactg taaaatagtc atgtttactt
     9421 tcatgttggt tggaatatcc gctcacttca cggtaatgtc ctttttcaaa accatttttt
     9481 cgaccagtta aaagcttatg accataagac tgatgggaaa catcccaaac gattttatcc
     9541 tttggtgaat caaatacata atgaaacgca atcgttaatt ctactacacc tagattcgga
     9601 ccaacgtgcc cacctacatc gctgtccttt tgcaagattt tttggcgcat ctcttctgcc
     9661 aactgtttca tttcctcaac agataatttt ttgacatctg cggggctatt gattgcatcc
     9721 aatacatgta atactgtact cataatacat tctctcctcc gtactatctt cttttaatga
     9781 aaatagattt gttacacttt atctattcta ctacttagag ttgactctaa gtcaatagca
     9841 ttttaaaaaa atttcagaga aagcttgtta tataagtaga tcttcgcacg aatcaggact
     9901 caaataaaaa ccttctacag acaattaaaa agctttgtag aaggtctcct aattaaggtc
     9961 tcatcaagaa atatgttttt ttaataggtg aagttcctga gcaatatgat acaaatggat
    10021 gtttgcacct tttaatgaga ttggatagat ctttgtcttt tcagtttcta atccattttg
    10081 atagctagca gcatcgcaca aaaccactct ttttttcgca catagacatt catcaggcaa
    10141 ttcttctaat gaacgaaata aaataggatt tatttcagcg atgccttctt ccgcatacaa
    10201 atgaaattgg gttcgataaa aaaattgttt gactgaactt tctgaaacaa caaaaatttc
    10261 tatttcctgt ctccccgctt gttcactgtt gatcaaagaa agacttttga cgaattttcg
    10321 aatgaaattt tcattccatc ttagttggat ttgatttttc tttttccact cttccaatat
    10381 ttctacaatt tttccgtaca gaagttgttc tttttctgat agaagataga tcctttccgg
    10441 taaaaatact tgtccatctg cccacaacgt acggacaaat tgtaaaaagg attgcttgaa
    10501 aataaaattt tctgcatcgt cgatttcagc gatactaata atctgtaaaa tcaattcatt
    10561 cagtgatagg tcattgagaa caaaattttt ataaacaact tcgaaatctt ttcttaacag
    10621 ctccatgttc tcattggtat aatttctact gttgaacaaa gaaaaaatat aatagacatc
    10681 ttcttcttgt aagtctagaa ttcctttttg ctcttccaat ccttgacaga ctaattgaaa
    10741 taacggcttc gtttctgcgc ttttcttatc ttcttctttc agtccaaggg gaaatgcctt
    10801 tcttccgatt gcgatctcta ttcctcgtat gataaaggac ttttcatctt ccgaatatct
    10861 tcttttaaaa aaatgagtct ctacataatc aatcaactcg ttacaagctt cttctacata
    10921 cggattttta ttttcatagc cttcccaatt gacataacgt actaaagcta gcaagatgtt
    10981 ccgcgagtca aattctggga taatgatttc tttatttttc aaataatcat tttctttaaa
    11041 aaaatccaat acgatttttt tgattgtata tactttgctc aaagaaataa attcaagatc
    11101 agccagttct gtactcgtga atataccatt aaaataataa cttaaaaact ttaaaaaata
    11161 agattgaccg tacagaaact gtgtgagctc agaaagagca ataccatgtt tgaatacgag
    11221 tttatactta tgcttctctc ttacttgttc ttttatacaa tattctagac actcggctag
    11281 actttctaga tcgtgaagga tcgttggtgt agtcaccctt agagaaatag ctgtttgttc
    11341 gatgtctatt tcttggaatt caaataattg ttcacacaga tacacttttc taaatatatt
    11401 tcgctcgatg taattttcca gcattcctga tacacgcctc cagtttttct tatgatttta
    11461 tttaatcata tctgtaaatc gtatttttgt ttccttttta tttagaaaag ccctttcaaa
    11521 aaccaataaa aaaagaaact gggacaaaag tcaaacaagc tcacaatttc agcctcattg
    11581 taggtagctt tgagccgttt ggacttttac cccagcctct ttcaagaatg gttattcaaa
    11641 gatgtcagcg tctagttctt tgactacatc tttcaacaat tggataccag ctgcgctgtt
    11701 accaaatgga tctagtgccg ggctgaagac acctatgccg taacgatttg gcacagctgc
    11761 catcaacccg ccgcctacac cgctttttgc aggtacacca acatggacgg aaaactctcc
    11821 tgattcatca tacaaaccag cagttgtcat aattgatttg acgattgtag cactttcttc
    11881 agtaatgatt tgtttgccat tccaaggagc aattccttta tttgccagca cagcagctaa
    11941 ttttgctatg cctgtagcgg tgacattgag ggagcattgt ctgaaataag catcgagtat
    12001 ctccggtacg tctccttcga tcatttgatt acctttcata tagtaagcaa gagagcgatt
    12061 gatatcacct gttcttgatt cagaatgata gatttcttca ttcaatgtga tattaggatc
    12121 atcacagatc tctttcataa atgacaagat tcgctcaaat ttttcatctg catctttacc
    12181 ggcaatcaac gaggttgttg caatcgctcc tgcattaaca aaaggattca ttggatgttt
    12241 gcgatgattg atttccatat tcatgataga attgaaggca aaaccggttg gttccgtgtt
    12301 gattgtttga aagacttttt ctattccgtt gtctttgatt gctaataaca atacgggaac
    12361 ttttgacatg ctttcgatgg caaagcgcac atcggcgtct cctccctcaa tcagttgttt
    12421 tgtatccaaa tcataaatcg ccatacctaa ttgtttggga tccacttcag ctaatgctgg
    12481 aatgtaatcc gccacttgtc ctgtttgata atagtggaaa ttttttccaa tcaattgttc
    12541 taattctttt acttccattt gaataaccag ctttcatttt ttatttttct gcatgctgga
    12601 atttatgacg gcatgcgtag atcgtaaatg gaataactaa gacgataaga taagaaacaa
    12661 tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat actagcaggt ggaacaaatg
    12721 agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca agcaacgatt acttttacaa
    12781 cttttccgcc tggaacctga taggctcttt caagactgtc atctttaaaa accagcttca
    12841 aatagcccgc gaaaaacata aagtacaaca tcaggtagat tacaacagtc aatgacatcg
    12901 ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt aaagacggct gcccagattg
    12961 ttgcaacgat cccatttgcg atcatcaaat gagcaggaac accgtgtgtg ttttcttttg
    13021 caaaatattc aggaagtaat ccttcttctg ctgctacgtg aagtgctttt gaaggagagg
    13081 ttacccaaga agcaatttct ccgatgacac ctaaacaaac aagaatcgca agaattttcg
    13141 caatccatgc aagatttgga tcaatgtgtc ttaataggaa ttctaacgat tgaatcaccc
    13201 ctgtattaag agacaaacca tgttgaggta ctgtagtagc gacggtcaat ccgccaaaag
    13261 tatctaaaat gattgccaga ataaccaata caaacattgc taatggataa tcctttttag
    13321 ggttttccat ttcatttgca tgactggcag atgtttctac gcccatatat gctaaaataa
    13381 aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc aggaatcaat gttttcatag
    13441 acagtatcgt ttgtagtgga gcgcctgaag cgacataatg gatccctaag ccaaacaaga
    13501 ctaatgctgg caacaagata ccagctacaa aaccgatccg agcaatcaat gaagtgtatt
    13561 tggttccttt taactgtgag agtaaaatag cccagaaaat aatcaatagg attgccaatt
    13621 tcagatattt attgttttgt atagcaccaa tacccgttgc ataggataat gcaccagtta
    13681 agaaatacag catggtcaag tagccaactg ttatctcaaa ccattggaaa aagattgcgg
    13741 caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt atagacaccg cctttttccc
    13801 atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac catagcaaac
    13861 cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg ggatattcat
    13921 ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt aatttttttg
    13981 caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg aatttgtttc
    14041 aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct tcttttgtag
    14101 tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc acataggcat
    14161 acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag ctgcttggat
    14221 tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct gtgaatccgc
    14281 ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt ttacgaaaat
    14341 caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca ctttttgctt
    14401 ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct gttagtacgt
    14461 cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta atcatcgcaa
    14521 atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct gctcgaagtg
    14581 caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg atagcagctg
    14641 ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga ccaagtgtga
    14701 ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca ccagcgaaat
    14761 ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa acagggaaat
    14821 aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc aatctccctg
    14881 caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg taaagaaaga
    14941 acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta acatgtctca
    15001 tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca aaggccaatc
    15061 cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa gattgagaca
    15121 tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct agaccaggag
    15181 tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct gtattgatag
    15241 gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag aaggtgtagt
    15301 tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca gacaattaat
    15361 aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca aaaaataaac
    15421 ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa agatattttt
    15481 tcataaggaa taaa
//
LOCUS       DAYYJC010000063        14669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000063 DAYYJC010000000
VERSION     DAYYJC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.063"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1448
                     /locus_tag="ACHA9N_001876"
     CDS             21..1448
                     /locus_tag="ACHA9N_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085331.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACHA9N_001877"
     CDS             complement(1540..1905)
                     /locus_tag="ACHA9N_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9085332.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACHA9N_001878"
     CDS             complement(1902..2267)
                     /locus_tag="ACHA9N_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9085333.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACHA9N_001879"
     CDS             complement(2459..2707)
                     /locus_tag="ACHA9N_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085334.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACHA9N_001880"
     CDS             2706..2834
                     /locus_tag="ACHA9N_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9085335.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACHA9N_001881"
     CDS             2891..4585
                     /locus_tag="ACHA9N_001881"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9085336.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4858
                     /locus_tag="ACHA9N_001882"
     CDS             4685..4858
                     /locus_tag="ACHA9N_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085337.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="ACHA9N_001883"
     CDS             5236..6369
                     /locus_tag="ACHA9N_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9085338.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="ACHA9N_001884"
     CDS             6400..6720
                     /locus_tag="ACHA9N_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9085339.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="ACHA9N_001885"
     CDS             complement(6749..7213)
                     /locus_tag="ACHA9N_001885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085340.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="ACHA9N_001886"
     CDS             complement(7345..8598)
                     /locus_tag="ACHA9N_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9085341.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="ACHA9N_001887"
     CDS             complement(8627..8944)
                     /locus_tag="ACHA9N_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9085342.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="ACHA9N_001888"
     CDS             complement(8970..9290)
                     /locus_tag="ACHA9N_001888"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9085343.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="ACHA9N_001889"
     CDS             complement(9754..11169)
                     /locus_tag="ACHA9N_001889"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9085344.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="ACHA9N_001890"
     CDS             complement(11186..12502)
                     /locus_tag="ACHA9N_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9085345.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="ACHA9N_001891"
     CDS             complement(12652..13350)
                     /locus_tag="ACHA9N_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9085346.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14628)
                     /locus_tag="ACHA9N_001892"
     CDS             complement(13639..14628)
                     /locus_tag="ACHA9N_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9085347.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         4600 a         2988 c         2520 g         4561 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag attaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttcttt
//
LOCUS       DAYYJC010000064        14602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000064 DAYYJC010000000
VERSION     DAYYJC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.065"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..993
                     /locus_tag="ACHA9N_001893"
     CDS             97..993
                     /locus_tag="ACHA9N_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9085348.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1143..2084)
                     /locus_tag="ACHA9N_001894"
     CDS             complement(1143..2084)
                     /locus_tag="ACHA9N_001894"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9085349.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2210..3331
                     /locus_tag="ACHA9N_001895"
     CDS             2210..3331
                     /locus_tag="ACHA9N_001895"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9085350.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3589..5091
                     /gene="eat(A)"
                     /locus_tag="ACHA9N_001896"
     CDS             3589..5091
                     /gene="eat(A)"
                     /locus_tag="ACHA9N_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9085351.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5158..5685
                     /locus_tag="ACHA9N_001897"
     CDS             5158..5685
                     /locus_tag="ACHA9N_001897"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9085352.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5896..6957
                     /locus_tag="ACHA9N_001898"
     CDS             5896..6957
                     /locus_tag="ACHA9N_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9085353.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6960..7856
                     /gene="murQ"
                     /locus_tag="ACHA9N_001899"
     CDS             6960..7856
                     /gene="murQ"
                     /locus_tag="ACHA9N_001899"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9085354.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7870..9852
                     /locus_tag="ACHA9N_001900"
     CDS             7870..9852
                     /locus_tag="ACHA9N_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9085355.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9917..10363)
                     /gene="nrdI"
                     /locus_tag="ACHA9N_001901"
     CDS             complement(9917..10363)
                     /gene="nrdI"
                     /locus_tag="ACHA9N_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9085356.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10574..11998
                     /locus_tag="ACHA9N_001902"
     CDS             10574..11998
                     /locus_tag="ACHA9N_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9085357.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12179..12952)
                     /locus_tag="ACHA9N_001903"
     CDS             complement(12179..12952)
                     /locus_tag="ACHA9N_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9085358.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12949..13854)
                     /locus_tag="ACHA9N_001904"
     CDS             complement(12949..13854)
                     /locus_tag="ACHA9N_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085359.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14008..14328)
                     /locus_tag="ACHA9N_001905"
     CDS             complement(14008..14328)
                     /locus_tag="ACHA9N_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9085360.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4665 a         2604 c         3095 g         4238 t
ORIGIN      
        1 aaattttcat acaaatatag gaaacgtttt actggctttt tactatttaa acaggctatc
       61 ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga acaaaaatga cgtactgcta
      121 gttgcaaaaa ataaatcact ttattttttg ccttccatcc gctctttaga tgtttggtcc
      181 ggaaaagatt tactacagtc ggaattcatt aaatcagcta gacaacagtt ggcaaattat
      241 cagctgatcg tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgccca
      301 tttaccaatg caaaaattgt gttatttttt tggaatcact tcaaagcaga acacgaggcg
      361 atgctgaagc aggcacaaga tgaaccggca atcgatgaga tttatcattt tgattttttg
      421 gaagcaaaag aacttgggct gaaacataat agttcatttt attcgaaaca tatgaatttt
      481 tcttctagcg aaccaaagac ggatctattt tttggtgcaa cgaataatgg gagaaaagaa
      541 cgagcagaaa gctataagca ggaatttata aaaagagatg ttacgaccaa ctatttcatt
      601 cttccacatc gagggaatga acaacctgga tatcttactt atgaagagta cctgaaaaaa
      661 acaggggaat cgcgcgggat tctggaactg ttgcgggagg gtcagcaagg agtgactttg
      721 cggacatttg aatccatgta ttttcaaaag aaactagtga cggataatca agcgattgct
      781 cattatcatt tttatcgtcc tgaaaatatt tttttgcttc gagaacgtga tttagatgaa
      841 ttaccggaat ttctgcatac accatttaag ccaatcctat cagagctatt ggaattttat
      901 gatgcagaaa attgggcaaa acgatttatc actaacgatt ccactattta tgagcaatac
      961 gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct acattttgaa gagattatgt
     1021 actgaaacaa acaagcacaa ttcttatggc atcatgagag ttgtgttttc tttttgatct
     1081 tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca tcagagagcc
     1141 ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa aaaggcacaa
     1201 tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt gatgtagtcg
     1261 atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta atgcaacccg
     1321 atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg atctagtatg
     1381 attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac catgctctct
     1441 gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca taaaccattc
     1501 aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg gctatagatc
     1561 ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc ttcgccttct
     1621 ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt tccgggacca
     1681 gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg cacatatttt
     1741 tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt attgtcagta
     1801 ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt taatgctacg
     1861 atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat tggcagtttt
     1921 gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat ggcttcaatg
     1981 tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag catcaatccg
     2041 attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt tctctccttt
     2101 attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt gttttgtttg
     2161 aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga tggatatttt
     2221 ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc aggcagttct
     2281 aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag atggcggaga
     2341 aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca ttgatgggat
     2401 ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc aaggaaaaaa
     2461 caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata aaccaacgga
     2521 tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag atgcactgct
     2581 tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg atggtggtct
     2641 aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa atcctttatt
     2701 atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag taaaattgat
     2761 tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt ggcaatttgg
     2821 aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag caggaaagat
     2881 tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag gaagtggagc
     2941 agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt ctggtttcga
     3001 tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg tcatcattac
     3061 aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg gtgtagcagt
     3121 gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag accaagagct
     3181 agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc caataggttt
     3241 agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag gacagacact
     3301 tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt tatgctatcg
     3361 taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag tcgattgatg
     3421 aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga ttttcgggat
     3481 tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg catcctcttt
     3541 agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat gtctaaaatc
     3601 gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt tgaacaagca
     3661 aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag
     3721 acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag
     3781 caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg
     3841 ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa
     3901 acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa
     3961 gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg
     4021 aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa
     4081 ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta
     4141 gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag
     4201 aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt
     4261 aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa
     4321 acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg
     4381 ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc
     4441 cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg
     4501 aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat
     4561 gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt
     4621 tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac
     4681 ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa
     4741 aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac
     4801 caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag
     4861 atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag
     4921 ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa
     4981 caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa
     5041 accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa
     5101 tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg
     5161 ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg
     5221 gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta
     5281 gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat
     5341 acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta
     5401 gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca
     5461 cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg
     5521 atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc
     5581 cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc
     5641 tttacattca tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt
     5701 atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga
     5761 caattctttt gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa
     5821 gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag
     5881 aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag
     5941 aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg
     6001 catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa
     6061 gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg
     6121 ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg
     6181 gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg
     6241 aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc
     6301 tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa
     6361 tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta
     6421 ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat
     6481 cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt
     6541 tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa
     6601 gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta
     6661 gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt
     6721 tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt
     6781 actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat
     6841 ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg
     6901 atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa
     6961 tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat
     7021 tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg
     7081 ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt
     7141 cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg
     7201 tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc
     7261 taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg
     7321 agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta
     7381 tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg
     7441 gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt
     7501 tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag
     7561 gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta
     7621 aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct
     7681 caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg
     7741 aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag
     7801 aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg
     7861 gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa
     7921 ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg
     7981 aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt
     8041 ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta
     8101 gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt
     8161 acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa
     8221 atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac
     8281 atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg
     8341 attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa
     8401 caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat
     8461 acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg
     8521 gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt
     8581 gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg
     8641 tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca
     8701 ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca
     8761 ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca
     8821 tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa
     8881 attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca
     8941 attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc
     9001 aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga
     9061 atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct
     9121 tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc
     9181 gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc
     9241 tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt
     9301 attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa
     9361 gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt
     9421 gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg
     9481 atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc agttactggg
     9541 gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta
     9601 gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg
     9661 aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa
     9721 gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt
     9781 gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat
     9841 gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc
     9901 agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat
     9961 cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat
    10021 attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac
    10081 caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga
    10141 tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa
    10201 aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt
    10261 caggattgac aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa
    10321 aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc
    10381 tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta
    10441 ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt
    10501 attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt
    10561 ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc
    10621 tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc
    10681 cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat
    10741 gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt
    10801 tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg
    10861 gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata
    10921 tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt
    10981 tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc
    11041 tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac
    11101 gttttcgatc caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg
    11161 acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt
    11221 tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg
    11281 gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct
    11341 tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg
    11401 ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat
    11461 catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa
    11521 catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc
    11581 aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt
    11641 cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg
    11701 gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt
    11761 tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa
    11821 tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg
    11881 taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt
    11941 gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa
    12001 gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga
    12061 aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag
    12121 acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc
    12181 atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta
    12241 aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg atctcttcgt
    12301 ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt
    12361 tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc
    12421 ctgctgagat ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta
    12481 aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg
    12541 tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca
    12601 acacagtgac aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc
    12661 atttgcttac taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac
    12721 catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc
    12781 aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt
    12841 ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag
    12901 ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc
    12961 ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg
    13021 ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa
    13081 ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc
    13141 tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc
    13201 tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat
    13261 cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt
    13321 gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa
    13381 accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat
    13441 tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc
    13501 catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa
    13561 cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc
    13621 tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt
    13681 tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc
    13741 attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag
    13801 ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat
    13861 tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc
    13921 attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt
    13981 tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat
    14041 actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa
    14101 gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag
    14161 actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat
    14221 cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca tcatcgttga
    14281 ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac ttacattcct
    14341 ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa
    14401 tgatcatcct ctttatccta cccggtggca gattgtatat ctttttctgt agtatagcac
    14461 acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc actgacggat
    14521 atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa gttgttattt
    14581 attaatcatt taaaaaaaac ag
//
LOCUS       DAYYJC010000065        14344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000065 DAYYJC010000000
VERSION     DAYYJC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.066"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..83
                     /locus_tag="ACHA9N_001906"
     CDS             <1..83
                     /locus_tag="ACHA9N_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFM9085361.1"
                     /translation="DTIVLHQRKLDLLKEQKKGFLQKMFV"
     gene            110..445
                     /locus_tag="ACHA9N_001907"
     CDS             110..445
                     /locus_tag="ACHA9N_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085362.1"
                     /translation="MKFLNFLNYKYLNWVLYGITLFFIIYYFFTDDDIFLILAILFLV
                     SSNFSDNLIEVLTEPEIRSMTDEEQQIIKAMISENKKDVVVIKKIREYTNLGVLQAKK
                     VYEKKKKGL"
     gene            622..1572
                     /locus_tag="ACHA9N_001908"
     CDS             622..1572
                     /locus_tag="ACHA9N_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9085363.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2500
                     /locus_tag="ACHA9N_001909"
                     /pseudo
     CDS             2090..2500
                     /locus_tag="ACHA9N_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2943..3755
                     /locus_tag="ACHA9N_001910"
                     /pseudo
     CDS             2943..3755
                     /locus_tag="ACHA9N_001910"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3750..3914
                     /locus_tag="ACHA9N_001911"
     CDS             3750..3914
                     /locus_tag="ACHA9N_001911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085364.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            3854..3976
                     /locus_tag="ACHA9N_001912"
                     /pseudo
     CDS             3854..3976
                     /locus_tag="ACHA9N_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(4538..5044)
                     /locus_tag="ACHA9N_001913"
     CDS             complement(4538..5044)
                     /locus_tag="ACHA9N_001913"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9085365.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RYWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRNSKILVPINILKSKISSLVSPLTEEL
                     QKIAMKVM"
     gene            complement(5049..5816)
                     /locus_tag="ACHA9N_001914"
     CDS             complement(5049..5816)
                     /locus_tag="ACHA9N_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9085366.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="ACHA9N_001915"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="ACHA9N_001915"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFM9085367.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="ACHA9N_001916"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="ACHA9N_001916"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFM9085368.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="ACHA9N_001917"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="ACHA9N_001917"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFM9085369.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="ACHA9N_001918"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="ACHA9N_001918"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9085370.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="ACHA9N_001919"
     CDS             9217..9534
                     /locus_tag="ACHA9N_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9085371.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="ACHA9N_001920"
     CDS             9534..11240
                     /locus_tag="ACHA9N_001920"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9085372.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="ACHA9N_001921"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="ACHA9N_001921"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9085373.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="ACHA9N_001922"
     CDS             12811..13083
                     /locus_tag="ACHA9N_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFM9085374.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="ACHA9N_001923"
     CDS             13104..14003
                     /locus_tag="ACHA9N_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9085375.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DAYYJC010000066        13943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000066 DAYYJC010000000
VERSION     DAYYJC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.067"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACHA9N_001924"
     CDS             136..309
                     /locus_tag="ACHA9N_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085376.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACHA9N_001925"
     CDS             949..1422
                     /locus_tag="ACHA9N_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9085377.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACHA9N_001926"
     CDS             1431..1658
                     /locus_tag="ACHA9N_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085378.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACHA9N_001927"
     CDS             2294..2665
                     /locus_tag="ACHA9N_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085379.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACHA9N_001928"
     CDS             complement(2921..3163)
                     /locus_tag="ACHA9N_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9085380.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACHA9N_001929"
     CDS             complement(3195..4097)
                     /locus_tag="ACHA9N_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9085381.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACHA9N_001930"
     CDS             complement(4110..4298)
                     /locus_tag="ACHA9N_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9085382.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA9N_001931"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACHA9N_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9085383.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACHA9N_001932"
     CDS             complement(4913..5806)
                     /locus_tag="ACHA9N_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9085384.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACHA9N_001933"
     CDS             complement(5884..6807)
                     /locus_tag="ACHA9N_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9085385.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACHA9N_001934"
     CDS             complement(6856..7206)
                     /locus_tag="ACHA9N_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9085386.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACHA9N_001935"
     CDS             complement(7239..8141)
                     /locus_tag="ACHA9N_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9085387.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACHA9N_001936"
     CDS             complement(8134..8991)
                     /locus_tag="ACHA9N_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9085388.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACHA9N_001937"
     CDS             9325..10134
                     /locus_tag="ACHA9N_001937"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9085389.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACHA9N_001938"
     CDS             10174..10671
                     /locus_tag="ACHA9N_001938"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9085390.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACHA9N_001939"
     tRNA            complement(11056..11129)
                     /locus_tag="ACHA9N_001939"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ACHA9N_001940"
     CDS             11316..11639
                     /locus_tag="ACHA9N_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085391.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ACHA9N_001941"
     CDS             11803..12057
                     /locus_tag="ACHA9N_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085392.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ACHA9N_001942"
     CDS             12127..12372
                     /locus_tag="ACHA9N_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9085393.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ACHA9N_001943"
     CDS             12448..12813
                     /locus_tag="ACHA9N_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9085394.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="ACHA9N_001944"
     CDS             complement(12874..13437)
                     /locus_tag="ACHA9N_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085395.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atg
//
LOCUS       DAYYJC010000067        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000067 DAYYJC010000000
VERSION     DAYYJC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.068"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA9N_001945"
     CDS             131..505
                     /locus_tag="ACHA9N_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9085396.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA9N_001946"
     CDS             complement(619..1248)
                     /locus_tag="ACHA9N_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9085397.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA9N_001947"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA9N_001947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHA9N_001948"
     tRNA            1668..1741
                     /locus_tag="ACHA9N_001948"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHA9N_001949"
     CDS             complement(1879..2043)
                     /locus_tag="ACHA9N_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085398.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHA9N_001950"
     CDS             2338..3666
                     /locus_tag="ACHA9N_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9085399.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHA9N_001951"
     CDS             3868..4701
                     /locus_tag="ACHA9N_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9085400.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHA9N_001952"
     CDS             4717..5454
                     /locus_tag="ACHA9N_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9085401.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHA9N_001953"
     CDS             complement(5609..6577)
                     /locus_tag="ACHA9N_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9085402.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHA9N_001954"
     CDS             6809..7642
                     /locus_tag="ACHA9N_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9085403.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHA9N_001955"
     CDS             7676..8515
                     /locus_tag="ACHA9N_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9085404.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHA9N_001956"
     CDS             8541..9101
                     /locus_tag="ACHA9N_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9085405.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHA9N_001957"
     CDS             9300..11021
                     /locus_tag="ACHA9N_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9085406.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHA9N_001958"
     CDS             11186..12328
                     /locus_tag="ACHA9N_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9085407.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA9N_001959"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHA9N_001959"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9085408.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYJC010000068        13905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000068 DAYYJC010000000
VERSION     DAYYJC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.069"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..170)
                     /locus_tag="ACHA9N_001960"
     CDS             complement(<1..170)
                     /locus_tag="ACHA9N_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9085409.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGF"
     gene            complement(649..1368)
                     /locus_tag="ACHA9N_001961"
     CDS             complement(649..1368)
                     /locus_tag="ACHA9N_001961"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9085410.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1417..3063)
                     /locus_tag="ACHA9N_001962"
     CDS             complement(1417..3063)
                     /locus_tag="ACHA9N_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFM9085411.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3242..3832
                     /locus_tag="ACHA9N_001963"
     CDS             3242..3832
                     /locus_tag="ACHA9N_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9085412.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3924..5429
                     /locus_tag="ACHA9N_001964"
     CDS             3924..5429
                     /locus_tag="ACHA9N_001964"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9085413.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5513..6865)
                     /gene="celB"
                     /locus_tag="ACHA9N_001965"
     CDS             complement(5513..6865)
                     /gene="celB"
                     /locus_tag="ACHA9N_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9085414.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6865..7383)
                     /locus_tag="ACHA9N_001966"
     CDS             complement(6865..7383)
                     /locus_tag="ACHA9N_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085415.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7399..7725)
                     /locus_tag="ACHA9N_001967"
     CDS             complement(7399..7725)
                     /locus_tag="ACHA9N_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9085416.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7738..9717)
                     /locus_tag="ACHA9N_001968"
     CDS             complement(7738..9717)
                     /locus_tag="ACHA9N_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9085417.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9738..10052)
                     /locus_tag="ACHA9N_001969"
     CDS             complement(9738..10052)
                     /locus_tag="ACHA9N_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9085418.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10220..10513
                     /locus_tag="ACHA9N_001970"
     CDS             10220..10513
                     /locus_tag="ACHA9N_001970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9085419.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10591..11703)
                     /locus_tag="ACHA9N_001971"
     CDS             complement(10591..11703)
                     /locus_tag="ACHA9N_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9085420.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11856..12704
                     /locus_tag="ACHA9N_001972"
                     /pseudo
     CDS             11856..12704
                     /locus_tag="ACHA9N_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13057..>13905)
                     /locus_tag="ACHA9N_001973"
     CDS             complement(13057..>13905)
                     /locus_tag="ACHA9N_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9085421.1"
                     /translation="APGAYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVE
                     TQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFE
                     LQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQE
                     VAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKF
                     RKKRNI"
BASE COUNT         4335 a         2839 c         2383 g         4348 t
ORIGIN      
        1 aagaacccat ctgctgaaat atgaaaattt ttgaatgcta cgacattcgc cattacaggt
       61 atgtaaaaga aaaaaatcat ccaaagcata cccggtacag ccatcaggac aagtgcttta
      121 taacgccata gattcataaa aaaatgattg aacccctttc gttttttcat ctaaaaaccc
      181 ctttcgattc attcgcaacg taattatagc gctttcaata atgagaataa ataaacaaag
      241 tatagggaaa atgaaacaaa gtataggaga ggttgtgaaa tcagcgtttt ggcctgagta
      301 ttagagaaaa aaacgggaaa atagttcctc atattttgcc gttttttgag ctaataccga
      361 gggtcagctg attgaacacc gtttatcaga ggttgtgaat tcagcgtttt ggcctgagta
      421 ttagggagat cgtgtcctta ctatctaact ctgagtcaca aggaacaatg gggactgtaa
      481 caaaagtctt gtcacagtcc cttattccga acaaacggtg ttccagaagt agcttctacg
      541 gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc agacggacaa gtctgctttt
      601 cagatggcgc tacaactact ctgcttattc ttttagctat ccattcaatt atttttctct
      661 cacttgttct agctcttctt tggtcatggg tcgatattgc cctttattca aatctttagg
      721 cagccaaaat tttcccatac ggattctttt taaatataag acttcttttc ctactgcttt
      781 gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa atcagacgga taattgaagt
      841 ttctgtctct tgatctactt gatcaatttg gagttttgcc ggcaatgtct gctcatcgcc
      901 atcaataatc agtccttctg caaataaacg gatatcttct tctgtcatta taccgctaac
      961 ctcagctaga tattctttct ccacatgttt tttaggtgat agtaactgat gggcaaacac
     1021 cccgtcattc gttaaaatca agaggccctc tgtgtcttta tctaatcggc cgactggaaa
     1081 aagatcttcc cgataatcct ggtcttccaa taaatcgatt acggtttgat catgattatc
     1141 ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg tagtaaaaat ctttttgata
     1201 cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat tcatttactt gcgctttatc
     1261 gcttttcacc acttgtccat tgacttcgat cgttcctttt ttgattaatc ctttgacttc
     1321 tttgcggctg ccaagtccca tatctgctaa aaacttatct aaacgcatgc gtttccccca
     1381 tttcaaaaga gcatcaatct agctcttctg attcacttat tttattttta atttatgtcg
     1441 taaacgcgct attcctggtc ctagtagtct ttctgccaag cggattttca aagctaaata
     1501 accatacact aaagccccta tacctgctac tacaagaatc aacaatagag actggaattt
     1561 gctatcttcg tttaaaaaag caccaaaaat catctttgtg atccacgaag ccaaaagcat
     1621 aatcaaggta agaataaaga tcagcaatgt cctgcgccag acaaaacttg gtttaaagcg
     1681 ggctacttca tggattctct ttaagatcaa ggtacaagac acagcaaatc cgatcattgt
     1741 cgccaataaa gggccgtata cttcaaataa acgaatggct gggtattgaa gaaggagttt
     1801 cactagaaac ccaataacca aataattaat tgccgccttg ttttcaaaca ttccttgcag
     1861 catgttggaa actagcatat atagtcctag gaagagtcct acaaaacttg cttggatcag
     1921 tacattactt cctagagaat ccggtgtata aaataatgta tttagcggat aggcaagtaa
     1981 cataacacca aatgtcgccg gaaacataaa aaatgaaaac aactgtaaat ttccgcttgt
     2041 caattttgcc agacctactc gatctttcaa cgtaacagct tctgtaatca acggtaaccc
     2101 agtagacgcg atcgatgttg ccaatgcaat aactaccatc gtcaacttat ctggattggc
     2161 actaaaaatc gaaaaaagat ccagtaactg tgcattggaa tactcggtgg atgcagacat
     2221 gatcttcata aaagtgaact gatcgactaa tttgaagatc gttacccctg aacccacaat
     2281 aataaacgga atcgcttctt taacggtatc taacaacaat tccttcgttg caatatttat
     2341 tttgttttcg ctgtagtgaa taaacgccga tgtatacgct tgatgtttct tcaaaaaata
     2401 caaaaggaca agaatacttg ctagcatacc gataaatgcc gcgaaagttg attgcgtgac
     2461 cgccgtcaca tagtcaccat ccatgacttt catgatgatg aatgtagcca atagcatata
     2521 aaaaatacgt gccacctgtt ctacaatctg cgacagcgca taaggcatca tttcttgatt
     2581 gccttgcaaa taccctcgta tcacactcat acaaggaaaa accaatacag cgatactcaa
     2641 agaacgcatc gttggaatca gttcttctcc gccaccagaa gcatgagcta gccaaggaga
     2701 ggcgatgtac atgaacaaag caaacaacag gcctaacccg cccatcatct gtaatgcacg
     2761 gataaataat cgtcgtgaag tgccatattc attcaatgaa ttatagcgtg cagtttgttt
     2821 tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga aacaatgcat atatattgta
     2881 tcccatattg aataatccat tggcagcctt agcattttcg cccatccaag cataccaggg
     2941 aatgatatag acagcgccta gcaatcttga aatgatatta ctggcggtca tccaagcaga
     3001 gcctcgtgcc attttttctt gcggtgttaa ttgatctaat gctggttttc tttgattatc
     3061 catcggtcag attccaactt tctacaaaac attactatca ttttaattgg taaaacagaa
     3121 actggcaatc gattttatat aacttttata agaacaattg tttttttgaa ttcggtttcg
     3181 tttttttggt aaagtaaaga aatatacagc cttttatggc taacatttat aggagatatc
     3241 aatgacacaa gaaataaaaa aactacccgt tatattttta ggtcttttct ttatcacagt
     3301 tggattgaat tggttcttgc tccctcacga tatagcttcc gccggagtgg gcgccatcgg
     3361 acatttagtt gaaacggcct tttctatcca acgctcagtt actgtttgga gcatcaatct
     3421 tatgatgctg cttctagccg ctgtcctatt aggaaaatca gtatttttta agactgtagc
     3481 gggaagtctg ctttttcctg ttgttttaga gattgttcca aaagtggaaa tgatctcctc
     3541 acatttcttt tcactgattt caggaagtct tttgttcagt cttggggttt acacattgta
     3601 cacagtcggg gcctcaaatg gcggtataac gataccgccg attattttcc acaaattttt
     3661 tcggcttcca atgccgcaag gacttttact taccaatagt cttatcgtct ttttgaatta
     3721 ttatgttttt ggattgttgg agacactatt cgttctccta tctattacag tcagttcatt
     3781 gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga aaatttgaat aaagctggat
     3841 tttgtcatag cggaaacaaa cgatatatta aaaagagtgt gctataataa accaagaaat
     3901 tgaataaagc gagggatcaa tcaatgagct tctctttaga agacatccgt catttactat
     3961 taaaagaaaa tctattaaaa gaatttgttt caacaagagg ctggcatctt gatgtaccag
     4021 aaaaagctga atttcttcag ttaagttatg attcaagaaa agctgatgct tctactttgt
     4081 ttttctgtaa aggaatgaat ttcaaagaag aatatttgga ttcagcaatc gaacagggta
     4141 ttcaatatta tgtatcagaa caaccatacg aaaattcagc agtaggaatc atcgtaacgg
     4201 atatccgtaa agcaatggcg cttcttgcta tggcttttta cgactatccg caaaataaat
     4261 tgaaagtgat cggattcact ggaacaaaag gtaaaacaac tgctgcgtac tttacaaaag
     4321 caatcttgga tcacacaaca aataaaaaaa cagcattgct ttctactatg aatacaacgt
     4381 tagatggcaa aacatatttc aaatcccatt tgacaacgcc tgagtcatta gatttatatc
     4441 gtatgatgtc agaagcagtt gaaaatggta tgactcatct agtgatggaa gtttcttcac
     4501 aagcttacaa aacacaacgc gtatacggct tgactttaga tgtcggaatc ttcttgaata
     4561 tctcgcctga ccatatcagt ccgatcgaac atccgacatt tgatgactat ttctattgca
     4621 agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa tcatgaaagt gactattttg
     4681 atttacttag ggaaacagcc gatctattcc agattccaac gatcacttat ggtcgttcgg
     4741 aagaaagcaa ttatcaagtg atccgttcag ataaaggtac tcacggtttt actttatctt
     4801 ctcatgaaga tcgcttagct atctgcgata ctgcttatga tattttgctg gctggaggat
     4861 tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc actaacaggt gcctccagtc
     4921 aagatgcaca agacggatta aaagaagctc gtgtaccagg ccgaatggat caattgatcc
     4981 aatccaatgg agcgcatgtc tatgtggact atgcacataa ttatctaagc ttgaaaacat
     5041 tgctgaaatt tgccaaaaat gaacatccag atggccgtgt aattgttgta ttaggcagcc
     5101 caggaaacaa agcaatctcc cgtcgacatg attttggaaa agtgctatca gaaacagctg
     5161 acatcgcctt tttgacggca gatgacccag catttgaaga tccgaaaaaa atcgcggaag
     5221 aaatcaatga agcaattact aatccagatt tgatcgttca ttatgaaatg gatcgtcctg
     5281 aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga ttcagtcgtt attgcgggaa
     5341 aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga accttatgaa ggggattatg
     5401 caatcgtgaa gcggttgatt gaagaataat ggtatttata tactagtggt ttcccatttc
     5461 taagaaaaaa agggactgtg attttttgtc acagttcctt ttttcttaga aatcatttta
     5521 acttttcact agctaattct gctgcctttt ctctttcttc atttaataat ttattatcat
     5581 agaatttgat aaatgggaac cagataatga atgcgatgat tgcacaaatg actgctaaca
     5641 cagcagcttt ccaatcacca ccactaccaa caaatgctcc gatcccgact ggtgaaggcc
     5701 acggcatctg tgcaagcatc ggtttgacga tttccagccg aatggcaaaa taagcgagtg
     5761 tagcagaagc catcggagct aagaaaaacg gaaccgctaa atagggattg tagacgattg
     5821 gcatcccgaa aatgattggt tcattgatat tgaataatcc tggtacaagt gaggcttttc
     5881 ctaacacttt cagctgatca gatttcgcga ggaatgcaat aaagatgatc aatcctaaag
     5941 tcgcacctga tccaccaacg gttacataag aattgttgaa ttctcctgcc aacggataat
     6001 ttgcgccttg tgcgttagct gccatattag ctaatacgat cggggtcaag aaagaagtga
     6061 tgatattcgc tccatggatc cctacgatcc acaatgcatg gatcaagaaa taaatcacca
     6121 tgacaccaag ccatgtattc gtcaactgcg taacaaaccc aaatgggata gcaatcattt
     6181 tgaagatatc cgttcctagt gcaactaata atccattgat gatcatcacc acaaacgcaa
     6241 tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac tcctggcgga acaacatctg
     6301 gcatttttat gacccaattt ctcttgacac acatacaata caattcgacc gcaataatcg
     6361 acataataat agctgtaaag attcctgaag ttcctaaacg ttcgacaaaa gagcccatcc
     6421 taataccatt tactacagtt tctgtctcat tgatagaagc aatcaagctg attttaccat
     6481 cagtcatgat caattcaggg atacacataa agaatgcaaa aacagatagt aatgcccccg
     6541 tgagcggatt aacatttaat ttttgttcat ctcgttcaat tgacgttaat tcataagcaa
     6601 acacaatggc aaagtaaata gataaaatcc ccatcgtcat tgtattgacg atcatgtaca
     6661 agtcactaac tttgaaaaaa gttgcattaa aaaatccttc taaaaatggg aacggtaatg
     6721 gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt gaaaggaata gaagccatcc
     6781 ccgctgccat gacagcccga acgattttaa gttgcgcaat tttccccata ggtcccatca
     6841 ggtatttctg caaaaaatca aacattagtt attctccttt cctaaatata acgctttttc
     6901 atattgttta atacggatat actgttggtc ttggttgagt gaaattttct ttaaacgata
     6961 ctgtataaat gcactggcaa aaataccgct tacaacaaga atgaaaacaa ataactgtcc
     7021 tccattgtta tttgccataa aaggatacaa gaatgtaaat cctgaggtaa acaacactgg
     7081 aatcactaat aaattaaaga cactctggaa caaaaaatac cactttgtcc atggaacgtt
     7141 cgtttggtgt tttcgataaa gctttacttg ttctcctact gcaggcaaaa tagctaaaat
     7201 cagaatcatc ggtaaactac ttcctaaact tttagctgat agaagcagaa tcatccattg
     7261 catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg attagataac gattgaaata
     7321 catgtttttc aaactaagtt gctttttttc taattcgatt ttccttttta ctgtatcctg
     7381 cacaatgatc cttcctttct atttattttt ttgatacaaa taattcattt cctgtgcaac
     7441 ttccaataat gtcatcgtcg tcatcagatg atcttgtgca tggaccatga taatttccat
     7501 ttctattttt gtcccgctgg catattcttt cagaagttga gtttgtgact gatgcgcatg
     7561 aagaatctct tcatttgctt gtcgcaactt ctcttctact tcttcaaaca tctctttacg
     7621 catcgagcga aatgcttcat gtatcagtgt ccgtgcattt ccgctatgga aaatgatatc
     7681 aaaagaagtc acttgtagtg tttcactgtt tattttttca gccatctgtt ttcctcctta
     7741 ttcgatcagt ttgataaatt tactgctgaa attctcaaaa ataggttcag ctaagatttc
     7801 ctgctgtact tttagctggt caactaacct aacgattgct ttagtcacga ccgtaattcc
     7861 ttcattttcg acataagaag gcgaaactaa aaaaacaaaa tgaatttctt gatattgatc
     7921 ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt gtttcgattc caactggaac
     7981 agcgggatgt gggactgcca ctgtatcact gaatatgatt tgtcccatat tttcacgttg
     8041 ctggatctgc tttcgcattt ctcttatata tccttcttct tcatttatgc tcagcttttg
     8101 cagcaaatca tgaagaattt cttccttttt cggttcgtac gtataagtct tgaaaaattt
     8161 ttcagacaac tgttcggtgt aaatctgctt tttttgctgt agagaaaatg ctggtatcgg
     8221 tctatctgag gaatgatagg gtatagattc tccaactacc tttcgtattt tacgaacatc
     8281 atcttcattc aaaaaaatac tgacatgtaa aactggaatt ttgaaaaaca ttgtcgaaag
     8341 atcaatagaa gaaataatca ggtcgattcc ttttaaagtt gtttcattga tctcataata
     8401 gccttttaca cttaccactg taatattttt accgaattca ctgactacgc gattctttaa
     8461 tagttgggca ctgccgtaac ccgttgcaca gataatcaat gcatgtagtt tatgcgcagc
     8521 tctttctttt tccaatgccg ccatgagatg caaagccaaa taggcccatt catcttcatt
     8581 gatggtatag tttttcaaag ccggcatgtt gctgaaatgc ctttttactg cgtcaaagac
     8641 tttgggattt tctttcttta tctcctcagt caatgggttc tcaatgcgaa tacctcggct
     8701 caatcgaatc atcatcggtg acaaatgatc gagtagtcca ttgaaaagct ggagatcctc
     8761 tactaatgaa aaatgaaatt cctgagacaa tcttttcaga actttcgcta attcctttga
     8821 tagttcctga tcattttgat tttctttgtg attcgactta gccatgagat gtaatgtcaa
     8881 ataagcaatt tcttcttcgg gaaacgatac acctgcgatt acctcaatac gttgaacgat
     8941 ccgctgcgcc acttgatact ctactcgctt agaaacttct tgttcgatgc ctaattcctt
     9001 gatttcaaac ccttctcgga gccgtttgat ccccaaagcc aagtgtaaag tcagattctg
     9061 tatgataaaa tccgaaactt ttaacttagc ttctcttgtt tcatcgagaa tgataatgac
     9121 tagctcttca aaactgatgt ctgggaaaaa acggctttgc cctaaatatt ctttcaatga
     9181 atttccataa tggtttccaa aaaaagtatc catgataaaa tgccgtcgat ttctttcttc
     9241 cccttcgatc catgtccctt gaccatgctt agaaacgatt tttagtgaat atggaatcaa
     9301 tcgctccttg atttcttgca tgtcctttgt caaactggaa cgactgatat aaagctcttc
     9361 tgccaaatca tccataaaaa gaatggcatc ttccaaaagc aattggttca atatatactt
     9421 ttgtcgatct tcggactcat aaaatccttg cggttctcta gcatatccag gtactatttc
     9481 ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa atatgcagtt ggtagccata
     9541 acctggttta gcaatgatct tacctccgtt tttttctact aactctttga tccgctgcaa
     9601 gtaattacgg attgtccgat cagataataa caattcagaa gctaaacgtt gactggtgac
     9661 atactccttc ttgtgatcaa tcaaatatct caacagcaat ttttccttct gtttcattat
     9721 catccactcc tattccatca tatgttctct tcgatcacct ctgctaactt ctggatgcct
     9781 gtagggattg ggacatacgc ttggaaaggg atactaacaa caggctttcc aacagattct
     9841 cctaattttt tgaatttatc caaaaacatc gtcgtttgcg ggctgatcag aaataaatca
     9901 aaatcactat tcttaatcat attatcgcct tctgtagctg aaactgcatc tacgagtacc
     9961 tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg ccatcatcga ggatgacatt
    10021 cctgccgcac aaataatcaa tgcttttttc ataaatcaat cactcctttt atttttataa
    10081 accaagcata tcttgtaagc gttttctatt caaaatatat atttccgtag cattgcggaa
    10141 aaaatgtgta tcatatcttg aataataatt tctggcaccg tgatatacta gtgatagaat
    10201 tttttcagga ggggatttag tgagtatcac agtgaatatt ttgtatacaa gtacaaatgg
    10261 acaagccaga aaatttgcag aagaaatggt cgaaagaaac attgtcaaag cgattcgtgc
    10321 agaagaagga aacgagaaat atgactatta ctttccacta gaagatccag aatctctttt
    10381 actgatcgac cgttggaaag atgaagaagc aatcgaaaag catcacaaat ccgaaatgat
    10441 ggctcaaata aacgaactcc gtaaaaagta taaattaaaa atgaaagtcg aacgtttcac
    10501 tgatttaccg taaaaagata cacatggaat gatagcaatt tatcatccat gtgtatcttc
    10561 actttttcct tattttttat tcgatctcat ctaatgattc aaaccgtgtt tttctcttat
    10621 ttttcggata agtatttcag tcaccccaat atacatgatt cctaaacata ccccaaggat
    10681 attctcaaaa atgcggatcg ttactgctcc tggaaccccg aataaactgg cagtgattgc
    10741 caaagcacca aaagaattaa aaactgtttt atatctatag gtagaacaaa cacctaaagc
    10801 tagaccacct aatataccga cccagttaaa aggaataaat tggctgatac caataaataa
    10861 aacacatcca atcaatgagc caatgattcg gtctactgct cgttctttga aaccaatcga
    10921 catcaatcca tagctagata caatagaaga aaatgcaaac cctgcccaca tcaatcgctg
    10981 aaaagggaca tactctcctg caacgatcaa aagactaatg ccaaatgcat agatgagcat
    11041 ccaaattttt tcctgcgaat acatgccttt caaaaaaatc tctttaaaca agcttttgtc
    11101 tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa aaccaacaaa aaaacaaaaa
    11161 taataagcta cttgtttgtg taaagaaatt cttgttcaac gaatctttag gtaacgaata
    11221 aacaataaag agatatgaaa agccgtacaa agaagcattc cccattttgg gatcactagc
    11281 ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc aacatgccca taactgacca
    11341 tttgatcagc tggacatacg gtgctactaa tagtgagaac atgacgattg ctaaacctat
    11401 gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga atactcatca acatacagaa
    11461 taaaaccaca gcgtatggtt tatgcttcga ttcaaataat gttgtaatta taatgataaa
    11521 acaaatggca aacaatacga tgaataatga tcgtaaaaac aatgaacaaa tatacatgca
    11581 cttttctttt ttagaagctg cttctcgtat tttttgtttt aaaataaacg gatctagttg
    11641 tagagactga taaaaatcca cctgttgtcg tcctttcttt ttacttttat tttttacagt
    11701 catctatgac aggttgtaaa tactatggaa catcatatgg cgttgtcatt ttcttgtcaa
    11761 ctatttttcc tattcatgtt tttataaaaa aacatatcgt tttcttgtat gattaaatca
    11821 ccacaaaata aacaactgag agaacgatat gctttatgaa tggttctatc aaaaaaattc
    11881 tgagatcaat agacaaaaat ttgatgatta cagaggtctc ttacgaaacc ttacagaaga
    11941 aaaaaacatt gatcgttgct gcagttctct tccctgctcc atgtgtctgt agaaattgtg
    12001 gttctgctgt ggtagatgga aataggaaat taatggtggt gaaaaacaag aagaaggaaa
    12061 ccattgtcca ctttgaataa tataatcata tgccaatggt tatacgccta aaaaagcagc
    12121 gctatacctg taaaaactgt cgaagccatt ggaacgctca aagttatttt atccgtccaa
    12181 ggcactcgat ttcaaatcat gtcagacata aaatcacttc tttactgact gaaaaagtgt
    12241 cgttattttt tatttcgaaa agttgttagg tatctttgac aaccgttatt cgcacattga
    12301 aagagttgag aagctattta ccaaagtctt ccaaaagaat tctgtcttga gtgttgaggg
    12361 ttgtgaattt cgttctcacg tttccgttga ggataaactg agttttattt ccgcggatgg
    12421 cgagacagaa aaattaatcg agaaaattta cctacacaaa aaattcctcg attaacaaac
    12481 tatttcctta gctgcactaa tcctaaagaa atagaattct tagttacaga catgaacgcc
    12541 acctactttc agctcaccaa acatgttcta ccaaatgcga aattggtgat tgatcgatta
    12601 cttggttttt ccacgccttt caaggaggcg taacctttat tcacgagttg gttgaagctt
    12661 tccgagacaa aagacccgac ttgtttttct ccttattgac aaagagccat taaaactaga
    12721 gaaaaaatta caactgatcg ctgattattt gtagaaagaa aatcaaagta ttacccaata
    12781 aacttgagtc attaggcaaa aaacactgcg aaactcaaga atgattttcg cagtgttttt
    12841 gtatattttt tattaactag tattactaca aaggtttatt tttcacttct caacgcagca
    12901 caaaattgtc tttacttatt tactggttgg gttcaacctt ctctactccg atactaatat
    12961 ttgtttgtat accccttcta aaatcggtag tttccattca attgatgggt actagttgat
    13021 tttaaaaact atgcttattc aattttttat tttagctcat atatttcgtt tctttctaaa
    13081 ttttatactg atgaaaacaa gtagcacacc caatattgac aatataaact ggctaatgat
    13141 ggtttctcca gtttttggaa gatgcttatt tccactgctt gttggattgt ttcctcttat
    13201 atctgagcta tcagcaactt ttctattctc ttttgttaaa aagatcgcca cttcttgttc
    13261 tttttcgatc ttgaaagtta ctggtgtcgc atccaattca taccccgttg gcgcttgagt
    13321 ttctaccagc tgatatgctc ctggtgctaa gccatctatc gccagttttc catctgctcc
    13381 tgtggttaga cctgactgca gaacggttcc attggcatct tgtaactcaa aaacagctcc
    13441 ttgcaggact tctccgcttt ggtcatcgat ctttgtcaac acgacaccgc ctggtgtaag
    13501 tcgattttct ttcgtcaatt ctaccaccgc tgtctgactt ctttcgatct caaactcgat
    13561 tggtgtcgca tccaattcat accccgttgg cgcttgggtt tctaccagct gatacactcc
    13621 tggtgctaag ccgtcaatcg ccagttttcc atctgctcct gtggttagac ctgactgcag
    13681 aacggttcca ttggcatctt gtaactcaaa aacagctcct tgcaggactt ccccgctttg
    13741 gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt cggttttctt tcgtcaattc
    13801 taccaccgct gtctgacttc tttcgatctc aaactcgatc ggtgtcgcat ctaattcata
    13861 ccccgttggc gcttgagttt ctaccagctg atatgctcct ggtgc
//
LOCUS       DAYYJC010000069        13841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000069 DAYYJC010000000
VERSION     DAYYJC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.070"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACHA9N_001974"
     CDS             139..477
                     /locus_tag="ACHA9N_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085422.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACHA9N_001975"
     CDS             complement(682..1257)
                     /locus_tag="ACHA9N_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFM9085423.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACHA9N_001976"
     CDS             complement(1989..2570)
                     /locus_tag="ACHA9N_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9085424.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACHA9N_001977"
     CDS             complement(2753..3379)
                     /locus_tag="ACHA9N_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9085425.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACHA9N_001978"
     CDS             complement(3401..4732)
                     /locus_tag="ACHA9N_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9085426.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACHA9N_001979"
     CDS             complement(4887..5288)
                     /locus_tag="ACHA9N_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9085427.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACHA9N_001980"
     CDS             complement(5374..5580)
                     /locus_tag="ACHA9N_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085428.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACHA9N_001981"
     CDS             complement(5675..7177)
                     /locus_tag="ACHA9N_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9085429.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACHA9N_001982"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACHA9N_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9085430.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACHA9N_001983"
     CDS             complement(7748..8365)
                     /locus_tag="ACHA9N_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9085431.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACHA9N_001984"
     CDS             8586..9959
                     /locus_tag="ACHA9N_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9085432.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACHA9N_001985"
     CDS             10034..11146
                     /locus_tag="ACHA9N_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9085433.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACHA9N_001986"
     CDS             complement(11218..12855)
                     /locus_tag="ACHA9N_001986"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9085434.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="ACHA9N_001987"
     CDS             complement(13023..13631)
                     /locus_tag="ACHA9N_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9085435.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3752 a         2900 c         2468 g         4721 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta t
//
LOCUS       DAYYJC010000070        13770 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000070 DAYYJC010000000
VERSION     DAYYJC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.071"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..2193
                     /gene="acm"
                     /locus_tag="ACHA9N_001988"
     CDS             28..2193
                     /gene="acm"
                     /locus_tag="ACHA9N_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9085436.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2465..2950)
                     /locus_tag="ACHA9N_001989"
     CDS             complement(2465..2950)
                     /locus_tag="ACHA9N_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9085437.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3040..3385
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3430..4071
                     /locus_tag="ACHA9N_001990"
     CDS             3430..4071
                     /locus_tag="ACHA9N_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9085438.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4090..4725
                     /locus_tag="ACHA9N_001991"
     CDS             4090..4725
                     /locus_tag="ACHA9N_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9085439.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4743..5558
                     /locus_tag="ACHA9N_001992"
     CDS             4743..5558
                     /locus_tag="ACHA9N_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9085440.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5877..7313
                     /locus_tag="ACHA9N_001993"
     CDS             5877..7313
                     /locus_tag="ACHA9N_001993"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9085441.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7502..9949
                     /locus_tag="ACHA9N_001994"
     CDS             7502..9949
                     /locus_tag="ACHA9N_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9085442.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10179..11246
                     /gene="rlmN"
                     /locus_tag="ACHA9N_001995"
     CDS             10179..11246
                     /gene="rlmN"
                     /locus_tag="ACHA9N_001995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9085443.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11236..11442
                     /locus_tag="ACHA9N_001996"
     CDS             11236..11442
                     /locus_tag="ACHA9N_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085444.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11439..12542)
                     /locus_tag="ACHA9N_001997"
     CDS             complement(11439..12542)
                     /locus_tag="ACHA9N_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9085445.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACHA9N_001998"
     CDS             13095..13643
                     /gene="aac(6')"
                     /locus_tag="ACHA9N_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9085446.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4716 a         2299 c         2914 g         3841 t
ORIGIN      
        1 ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg
       61 ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc
      121 agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga
      181 ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat
      241 tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa
      301 atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg
      361 caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt
      421 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
      481 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      541 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      601 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      661 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
      721 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
      781 gctacattta atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt
      841 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
      901 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
      961 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
     1021 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
     1081 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
     1141 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
     1201 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
     1261 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
     1321 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
     1381 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1441 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1501 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1561 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1621 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1681 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     1741 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     1801 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     1861 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     1921 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     1981 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     2041 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     2101 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     2161 gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata
     2221 tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat
     2281 atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg
     2341 tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg
     2401 atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct
     2461 atcattacaa attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt
     2521 cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt
     2581 gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg
     2641 ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa
     2701 caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt
     2761 catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt
     2821 ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat
     2881 cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg
     2941 cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca
     3001 aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga
     3061 gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt
     3121 gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt
     3181 acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa
     3241 aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt
     3301 tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg
     3361 gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag
     3421 gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca
     3481 acattgaaat tatttttatt taccttgatt ggctcattac cattagggat tgttttagca
     3541 ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt
     3601 atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt
     3661 gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct
     3721 tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca
     3781 gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg
     3841 acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc
     3901 ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc
     3961 gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt
     4021 ctaacaatca taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga
     4081 ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc
     4141 aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt
     4201 ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa
     4261 ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta
     4321 ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga aaacattacc
     4381 ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa
     4441 ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca gctttctggc
     4501 ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg ggtattggct
     4561 tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc
     4621 ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca
     4681 gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag
     4741 agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct
     4801 gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg
     4861 ttataggttt agacgataca tttgttccta tgggatttag agataaagac gggaagctca
     4921 caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact
     4981 ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga
     5041 tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt
     5101 atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac
     5161 aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga
     5221 aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt
     5281 ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag
     5341 tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag
     5401 gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga
     5461 aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg
     5521 ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa
     5581 gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc
     5641 ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg
     5701 tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac
     5761 tcgaaactaa gcgacttttt cacaacctcc tttacataat tttattttgt tgtggaaggg
     5821 gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag agatgaatgg
     5881 aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct tctgcagcgt
     5941 atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg gatcgatttg
     6001 tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta gaccactatc
     6061 atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct taccgcttct
     6121 ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa gctgggctcg
     6181 ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct atgttgactt
     6241 tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg gaatcacgtc
     6301 agatcattga agatttcact cattatgcag aaacattatt tgaggcattc cgcgggaaag
     6361 tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt tatttgttag
     6421 cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac catattgcta
     6481 atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg
     6541 gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa aatgttctgg
     6601 ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat atgtggggaa
     6661 aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca acgattgaag
     6721 ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc aattactatc
     6781 aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa atgaatacga
     6841 caggaaaaaa aggttcatca gaagaaactg gagttcctgg aatgtataaa aaagttgaga
     6901 atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga ttaaggatcg
     6961 gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa aatggcttag
     7021 gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga atcaattact
     7081 tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa gtcctaggct
     7141 attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa aagcgatatg
     7201 gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga tataagaaag
     7261 acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt tagttcacat
     7321 acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa aatcatttag
     7381 aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac
     7441 aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg agggataata
     7501 aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca
     7561 aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga ttttatttgc
     7621 tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg gctttcggat
     7681 cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag gattttggat
     7741 tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg gcggcatact
     7801 cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac tccatactgg
     7861 ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa aacaaagtca
     7921 agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt ttacttactt
     7981 cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag gaggagcatt
     8041 cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa ttggagaacg
     8101 agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag cggcaaagga
     8161 agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg caaaagaagc
     8221 tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa
     8281 tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc
     8341 aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac
     8401 accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac
     8461 ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt
     8521 tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa
     8581 aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa
     8641 agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt
     8701 aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg
     8761 gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat
     8821 cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact
     8881 agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat
     8941 gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat
     9001 tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc
     9061 gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac
     9121 gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata
     9181 tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca
     9241 gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga
     9301 tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt
     9361 agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt
     9421 ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc
     9481 aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg
     9541 aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt
     9601 catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca
     9661 ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc
     9721 acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat
     9781 ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga
     9841 actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat
     9901 tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga
     9961 tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa
    10021 aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga
    10081 gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata
    10141 gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg
    10201 gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag
    10261 ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga
    10321 atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac
    10381 agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata
    10441 aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga
    10501 cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc
    10561 gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg
    10621 agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg
    10681 ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa
    10741 ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg
    10801 ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc
    10861 gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg
    10921 atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag
    10981 taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat
    11041 tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca
    11101 gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg
    11161 tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc
    11221 aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa
    11281 ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg
    11341 aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat
    11401 ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct
    11461 tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt
    11521 tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag
    11581 attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg
    11641 ttctttttca taaacacata gattttatca aacaaatcga actgctgatc catcatcaat
    11701 aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga
    11761 acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa
    11821 acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag
    11881 ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg
    11941 cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca
    12001 gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta
    12061 tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga
    12121 aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc
    12181 gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg
    12241 acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg
    12301 acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag
    12361 agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt
    12421 gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca
    12481 tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc
    12541 atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg
    12601 actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg
    12661 catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga
    12721 aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt
    12781 gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca
    12841 attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt
    12901 tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat
    12961 aattttttta ttttataagt aagataaata atgaaagaat agagatactg attggtagct
    13021 aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac
    13081 aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc
    13141 agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag
    13201 aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt
    13261 tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat
    13321 tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag
    13381 aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc
    13441 atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca
    13501 tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag
    13561 gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta
    13621 ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga
    13681 aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc
    13741 gaagaagcta cttttggaac accgtgtacc
//
LOCUS       DAYYJC010000071        13429 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000071 DAYYJC010000000
VERSION     DAYYJC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.072"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHA9N_001999"
     CDS             complement(<1..914)
                     /locus_tag="ACHA9N_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9085447.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHA9N_002000"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHA9N_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9085448.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHA9N_002001"
     CDS             2006..2176
                     /locus_tag="ACHA9N_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085449.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA9N_002002"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHA9N_002002"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9085450.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA9N_002003"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHA9N_002003"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9085451.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA9N_002004"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHA9N_002004"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9085452.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA9N_002005"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHA9N_002005"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9085453.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA9N_002006"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHA9N_002006"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9085454.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHA9N_002007"
     CDS             6751..7752
                     /locus_tag="ACHA9N_002007"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9085455.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHA9N_002008"
     CDS             7749..9110
                     /locus_tag="ACHA9N_002008"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9085456.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHA9N_002009"
     CDS             9070..10221
                     /locus_tag="ACHA9N_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9085457.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHA9N_002010"
     CDS             10196..11365
                     /locus_tag="ACHA9N_002010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9085458.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHA9N_002011"
     CDS             11540..12919
                     /locus_tag="ACHA9N_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9085459.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACHA9N_002012"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACHA9N_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAYYJC010000072        13403 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000072 DAYYJC010000000
VERSION     DAYYJC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.073"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..2031
                     /gene="yfcC"
                     /locus_tag="ACHA9N_002013"
     CDS             292..2031
                     /gene="yfcC"
                     /locus_tag="ACHA9N_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9085460.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2188..3372
                     /gene="iadA"
                     /locus_tag="ACHA9N_002014"
     CDS             2188..3372
                     /gene="iadA"
                     /locus_tag="ACHA9N_002014"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9085461.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3483..3686
                     /locus_tag="ACHA9N_002015"
     CDS             3483..3686
                     /locus_tag="ACHA9N_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9085462.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3787..4449)
                     /locus_tag="ACHA9N_002016"
     CDS             complement(3787..4449)
                     /locus_tag="ACHA9N_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085463.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4462..5964)
                     /locus_tag="ACHA9N_002017"
     CDS             complement(4462..5964)
                     /locus_tag="ACHA9N_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9085464.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6512..8191
                     /locus_tag="ACHA9N_002018"
     CDS             6512..8191
                     /locus_tag="ACHA9N_002018"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9085465.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8318..8797
                     /locus_tag="ACHA9N_002019"
     CDS             8318..8797
                     /locus_tag="ACHA9N_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9085466.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9016..9696
                     /locus_tag="ACHA9N_002020"
     CDS             9016..9696
                     /locus_tag="ACHA9N_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9085467.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9704..11035
                     /locus_tag="ACHA9N_002021"
     CDS             9704..11035
                     /locus_tag="ACHA9N_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9085468.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11050..12012
                     /locus_tag="ACHA9N_002022"
     CDS             11050..12012
                     /locus_tag="ACHA9N_002022"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9085469.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12123..12461
                     /locus_tag="ACHA9N_002023"
     CDS             12123..12461
                     /locus_tag="ACHA9N_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9085470.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12540..13247
                     /locus_tag="ACHA9N_002024"
     CDS             12540..13247
                     /locus_tag="ACHA9N_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9085471.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4298 a         2348 c         2925 g         3832 t
ORIGIN      
        1 tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg aaaaaaacag gacgagattt
       61 tctctaatct aaaaataaaa aaagaatttt aaggaaaaac agagaaataa aatacttaat
      121 ataaatggaa agagaaaaaa ataatcatac ataggagaaa aaaaacaaaa tagaaaaaag
      181 tgattttcta ataaattatt catgtggaaa tgtcattttt actgtataat tcatttgatt
      241 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
      301 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
      361 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
      421 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
      481 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
      541 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
      601 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
      661 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
      721 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
      781 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
      841 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
      901 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
      961 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
     1021 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
     1081 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
     1141 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
     1201 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     1261 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     1321 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     1381 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     1441 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     1501 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     1561 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     1621 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     1681 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     1741 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     1801 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     1861 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     1921 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     1981 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     2041 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     2101 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     2161 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     2221 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     2281 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     2341 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     2401 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     2461 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     2521 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     2581 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     2641 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     2701 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     2761 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     2821 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     2881 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     2941 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     3001 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     3061 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     3121 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     3181 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     3241 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     3301 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     3361 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     3421 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     3481 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     3541 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     3601 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     3661 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     3721 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     3781 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     3841 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     3901 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     3961 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     4021 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     4081 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     4141 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     4201 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     4261 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     4321 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     4381 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     4441 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     4501 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     4561 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     4621 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     4681 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     4741 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     4801 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     4861 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     4921 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     4981 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     5041 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     5101 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     5161 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     5221 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     5281 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     5341 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     5401 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     5461 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     5521 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     5581 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     5641 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     5701 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     5761 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     5821 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     5881 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     5941 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     6001 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     6061 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     6121 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     6181 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     6241 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     6301 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     6361 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     6421 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     6481 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     6541 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     6601 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     6661 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     6721 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     6781 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     6841 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     6901 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     6961 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     7021 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     7081 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     7141 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     7201 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     7261 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     7321 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     7381 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     7441 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     7501 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     7561 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     7621 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     7681 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     7741 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     7801 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     7861 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     7921 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     7981 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     8041 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     8101 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     8161 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     8221 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
     8281 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
     8341 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
     8401 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
     8461 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
     8521 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
     8581 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
     8641 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
     8701 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
     8761 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
     8821 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
     8881 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
     8941 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
     9001 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
     9061 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
     9121 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
     9181 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
     9241 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
     9301 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
     9361 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
     9421 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
     9481 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
     9541 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
     9601 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
     9661 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
     9721 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
     9781 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
     9841 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
     9901 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
     9961 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
    10021 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
    10081 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
    10141 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
    10201 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
    10261 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
    10321 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
    10381 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
    10441 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
    10501 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
    10561 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
    10621 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
    10681 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
    10741 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
    10801 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
    10861 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
    10921 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
    10981 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
    11041 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
    11101 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
    11161 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
    11221 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
    11281 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
    11341 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
    11401 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
    11461 gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
    11521 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
    11581 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
    11641 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
    11701 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
    11761 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
    11821 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
    11881 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
    11941 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
    12001 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
    12061 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
    12121 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
    12181 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
    12241 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
    12301 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
    12361 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
    12421 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
    12481 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
    12541 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
    12601 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
    12661 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
    12721 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
    12781 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
    12841 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
    12901 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
    12961 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
    13021 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
    13081 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
    13141 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
    13201 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
    13261 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
    13321 tggaataagg gctctttgtc aataaggact gatgggttgt acaaaatcaa atctgggtca
    13381 gaatgaacct cattctggct cag
//
LOCUS       DAYYJC010000073        13346 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000073 DAYYJC010000000
VERSION     DAYYJC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.074"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..164
                     /locus_tag="ACHA9N_002025"
     CDS             <1..164
                     /locus_tag="ACHA9N_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9085472.1"
                     /translation="KFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRK
                     KAMRKRVSR"
     gene            complement(226..2037)
                     /locus_tag="ACHA9N_002026"
     CDS             complement(226..2037)
                     /locus_tag="ACHA9N_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9085473.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2034..2399)
                     /locus_tag="ACHA9N_002027"
     CDS             complement(2034..2399)
                     /locus_tag="ACHA9N_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9085474.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2562..2957)
                     /locus_tag="ACHA9N_002028"
     CDS             complement(2562..2957)
                     /locus_tag="ACHA9N_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9085475.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2975..3937)
                     /locus_tag="ACHA9N_002029"
     CDS             complement(2975..3937)
                     /locus_tag="ACHA9N_002029"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9085476.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3937..4149)
                     /locus_tag="ACHA9N_002030"
     CDS             complement(3937..4149)
                     /locus_tag="ACHA9N_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9085477.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4170..4868)
                     /locus_tag="ACHA9N_002031"
     CDS             complement(4170..4868)
                     /locus_tag="ACHA9N_002031"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9085478.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4888..5430)
                     /locus_tag="ACHA9N_002032"
     CDS             complement(4888..5430)
                     /locus_tag="ACHA9N_002032"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9085479.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5562..6566)
                     /locus_tag="ACHA9N_002033"
     CDS             complement(5562..6566)
                     /locus_tag="ACHA9N_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9085480.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6563..7552)
                     /locus_tag="ACHA9N_002034"
     CDS             complement(6563..7552)
                     /locus_tag="ACHA9N_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9085481.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7549..8355)
                     /locus_tag="ACHA9N_002035"
     CDS             complement(7549..8355)
                     /locus_tag="ACHA9N_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085482.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8521..9477
                     /locus_tag="ACHA9N_002036"
     CDS             8521..9477
                     /locus_tag="ACHA9N_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9085483.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9554..10072)
                     /locus_tag="ACHA9N_002037"
     CDS             complement(9554..10072)
                     /locus_tag="ACHA9N_002037"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9085484.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10160..10309)
                     /gene="rpmG"
                     /locus_tag="ACHA9N_002038"
     CDS             complement(10160..10309)
                     /gene="rpmG"
                     /locus_tag="ACHA9N_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9085485.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10537..10983
                     /locus_tag="ACHA9N_002039"
     CDS             10537..10983
                     /locus_tag="ACHA9N_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9085486.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11178..13073)
                     /gene="asnB"
                     /locus_tag="ACHA9N_002040"
     CDS             complement(11178..13073)
                     /gene="asnB"
                     /locus_tag="ACHA9N_002040"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9085487.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4155 a         2831 c         2276 g         4084 t
ORIGIN      
        1 aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc gttgctggtt
       61 tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa gcaatgatcg
      121 aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta tttaaaaata
      181 cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt tgtcagtcaa
      241 tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat aaggtctagc
      301 tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat aaaagtcaga
      361 ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat ctatagcttc
      421 tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt
      481 caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca atcgagcttc
      541 aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc ggttgtgctt
      601 ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc
      661 taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta gatgagtaaa
      721 ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt caatagcctg
      781 ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca
      841 attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag cttctgaaaa
      901 gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc tataagggat
      961 tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat catgctcaaa
     1021 atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat aaaacaactc
     1081 aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca
     1141 ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc cttgcttttg
     1201 atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata caatcggttc
     1261 cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga
     1321 tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta aaaagacatt
     1381 ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa gctgatacag
     1441 taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct catcattttc
     1501 ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat ctgtctgtaa
     1561 aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa actgaataga
     1621 agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc cgtataaaca
     1681 tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta gttgcttatt
     1741 tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt gaaacaagtt
     1801 gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa gcgtcacttg
     1861 ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa ctcgacctcc
     1921 aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat tattatggag
     1981 aatcctttct gctacactta atcctctagg tccggcacca atgatcccta ttttcatttg
     2041 catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg ttcatttttt
     2101 gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag tttaaaaata
     2161 gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc aaatgaagca
     2221 aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc aatttctttc
     2281 aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa tctatccacg
     2341 agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa tttgatcata
     2401 gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata gcacaaaata
     2461 aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt caaaatctaa
     2521 tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt tttacttttg
     2581 atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg ataacctgct
     2641 ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta aatatagaca
     2701 ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt
     2761 gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat tacttgtgct
     2821 tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt
     2881 actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa aatgatatta
     2941 attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag gctaatgagg
     3001 cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt gttgattctc
     3061 ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt aaaaattctc
     3121 ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta ttccccaagt
     3181 ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat gcaaacggat
     3241 cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg ttgtctagca
     3301 ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct aggcgtacca
     3361 cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta cattcgaacc
     3421 cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg ccgtgaatca
     3481 aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg aatacaacgt
     3541 ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca ttcgcgtcat
     3601 tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca aattgaactt
     3661 ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg
     3721 gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa ttcaaacgat
     3781 gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa atattggtat
     3841 cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat tttgctgttg
     3901 ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt tctttcggct
     3961 ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg tatcatgatc
     4021 aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa tgtcatagat
     4081 gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta catcatataa
     4141 gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt ttttaaaccc
     4201 agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat cctttggtgt
     4261 atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac taatccgcca
     4321 attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc aaaaacaaat
     4381 gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg gttatgttta
     4441 aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt acttgcacca
     4501 ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat tatcccgctg
     4561 aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg ctgccccata
     4621 aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat gaagattggt
     4681 gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat tccggcataa
     4741 taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt ctgtatccct
     4801 aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat ttgctgttga
     4861 taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga tcttctgatc
     4921 aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc tacatgtttt
     4981 tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga ttcgataacc
     5041 ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg gcaaatagag
     5101 cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg actgttcatc
     5161 catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta ttttcttttg
     5221 ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat tcgataaagt
     5281 cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt tatagattcg
     5341 ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt cgattccttt
     5401 ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct cataaaatgg
     5461 tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa tagaataaaa
     5521 agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt
     5581 ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc aatcaaaatt
     5641 cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc actaagcaga
     5701 aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt acgtacgact
     5761 agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt caccgctaca
     5821 ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt agccacgtta
     5881 aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct ctgtgcataa
     5941 agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac ataaggccag
     6001 ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt ttccttagat
     6061 aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc aataccggac
     6121 aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag atagacacag
     6181 caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg cagcgaatag
     6241 tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt aatgcccaat
     6301 atactagaat ctgccaattc atttcttgtt actccttgca agatataacc agaaacgcca
     6361 agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc tctaacaatc
     6421 agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc caggtaaata
     6481 aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa aagcaagaaa
     6541 tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc agtaaaaacg
     6601 gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga ttgatcatcc
     6661 tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag acaggtagaa
     6721 cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac agcccaataa
     6781 atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact agacagaaaa
     6841 caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt tcactcaaat
     6901 gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt atatataaag
     6961 gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca aaccaaaaag
     7021 caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca gtaaaaaaag
     7081 aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt ttcgaaaact
     7141 gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac attagaaaca
     7201 cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc aacgaagcac
     7261 ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc ccttgcatca
     7321 atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca cgaggcaaac
     7381 gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc
     7441 aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa aaaataatca
     7501 aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc ataattgatc
     7561 ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg ttcttggaag
     7621 cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg tgatcacttc
     7681 ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat aatctgaaaa
     7741 acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt tcatttgatt
     7801 gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg
     7861 ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc agacacgctg
     7921 ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta gacctgtagc
     7981 atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga acagtttttg
     8041 ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag ggtgttcact
     8101 agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg tagagatatc
     8161 agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa aacttttcaa
     8221 taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc cttcaggaat
     8281 cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa tgtcagctgc
     8341 tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt atgacgctgt
     8401 ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg ttgacagcgt
     8461 tcgaaatatc aggaataata accaatgaga aagattctca attatgttag gaggaaagaa
     8521 atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc tggagtactg
     8581 agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc gacgacttca
     8641 tccaatagta ctcatacagt cacagataca cttggacata aagtagagat tccaaacgcg
     8701 cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg ggaaaaacct
     8761 atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga tgatttaaag
     8821 gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt tgagcctgat
     8881 ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca atatagcaaa
     8941 atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga acaactaaaa
     9001 gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa
     9061 aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa gacagctgca
     9121 gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc aagtggaaga
     9181 atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt ctccaaaaaa
     9241 gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga tgctgactac
     9301 atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag ctggaagaat
     9361 ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag ttggctatat
     9421 aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact aaattgattt
     9481 taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta ttaacaaact
     9541 agacgcatag attttattct tcttttgaag caaccctatt ttttactctt tcccattttt
     9601 gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc tctataaggg
     9661 cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca
     9721 atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg
     9781 aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg atcgaatgca
     9841 ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc
     9901 gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc actaaaattc
     9961 gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat ggtaacaaac
    10021 catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac atattatccg
    10081 ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga ccagcaagga
    10141 attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg cgttcgcgtg
    10201 ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg cttgttagat
    10261 agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg tttttccctc
    10321 ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac catgattttt
    10381 tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg ataaatacaa
    10441 cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta cacttgttta
    10501 tatagattat gttttttaat aaagggggat actaatatgc catggaacat gaaggacttt
    10561 cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga catcgcaaat
    10621 gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat tgatcaagca
    10681 aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat
    10741 ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct tgattcagat
    10801 gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca
    10861 agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac
    10921 aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga aatcgattat
    10981 tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca
    11041 atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac gggaagagct
    11101 gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac ctctttttta
    11161 ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg cggaatcgtt
    11221 tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca gatttttctt
    11281 tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt ttcttgatca
    11341 aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta gccgtgttct
    11401 tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt cgaccattct
    11461 tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat
    11521 aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa taaaggtact
    11581 cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat atcttttggc
    11641 atccattgat gaagatcgac atattgcatc ttattgactt catctttgat tccttctgtt
    11701 ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata ttttggtgtt
    11761 aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa atattctttt
    11821 gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat ttttccgatt
    11881 ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg agcaatcaca
    11941 cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa cagttcatcc
    12001 gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc taaaaagtac
    12061 aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat caaagggaat
    12121 gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc caatagttcg
    12181 gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc gattgagaaa
    12241 gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct ggataagaaa
    12301 gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg
    12361 attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa
    12421 tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc aggcaaacga
    12481 tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt catataaggt
    12541 ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa acttttgatt
    12601 tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt gatcccaaag
    12661 tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa agcaaacatt
    12721 ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa aaggacttct
    12781 gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct caaaggctgg
    12841 taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt gtaaataggc
    12901 tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc aacatgttta
    12961 tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat cattgtatta
    13021 ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca catttaactt
    13081 tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc atagaataaa
    13141 tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt
    13201 ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt ttttgaggct
    13261 accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa aaaccagcaa
    13321 aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DAYYJC010000074        13051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000074 DAYYJC010000000
VERSION     DAYYJC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.075"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..1418
                     /locus_tag="ACHA9N_002041"
     CDS             117..1418
                     /locus_tag="ACHA9N_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9085488.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1445..1705
                     /locus_tag="ACHA9N_002042"
     CDS             1445..1705
                     /locus_tag="ACHA9N_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9085489.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2704..3939)
                     /locus_tag="ACHA9N_002043"
     CDS             complement(2704..3939)
                     /locus_tag="ACHA9N_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9085490.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            4507..5334
                     /locus_tag="ACHA9N_002044"
     CDS             4507..5334
                     /locus_tag="ACHA9N_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085491.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(5607..6377)
                     /locus_tag="ACHA9N_002045"
     CDS             complement(5607..6377)
                     /locus_tag="ACHA9N_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9085492.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            6595..7662
                     /gene="ulaG"
                     /locus_tag="ACHA9N_002046"
     CDS             6595..7662
                     /gene="ulaG"
                     /locus_tag="ACHA9N_002046"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9085493.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7680..8141
                     /locus_tag="ACHA9N_002047"
     CDS             7680..8141
                     /locus_tag="ACHA9N_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9085494.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            8161..9645
                     /locus_tag="ACHA9N_002048"
     CDS             8161..9645
                     /locus_tag="ACHA9N_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9085495.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            9688..9987
                     /locus_tag="ACHA9N_002049"
     CDS             9688..9987
                     /locus_tag="ACHA9N_002049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9085496.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            10002..10640
                     /locus_tag="ACHA9N_002050"
     CDS             10002..10640
                     /locus_tag="ACHA9N_002050"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9085497.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            10645..11505
                     /locus_tag="ACHA9N_002051"
     CDS             10645..11505
                     /locus_tag="ACHA9N_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9085498.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            11495..12205
                     /locus_tag="ACHA9N_002052"
     CDS             11495..12205
                     /locus_tag="ACHA9N_002052"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9085499.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            12437..12871
                     /locus_tag="ACHA9N_002053"
     CDS             12437..12871
                     /locus_tag="ACHA9N_002053"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085500.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         4150 a         2045 c         2731 g         4125 t
ORIGIN      
        1 gattatttaa gagaatatat tgaaataaaa tttcttaatt atataattag ttatgaacat
       61 tctttgcgtt gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg
      121 aaaagctgat gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac
      181 agcgtcattt attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg
      241 ggacattttt tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc
      301 cttattcggc aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg
      361 caacttttgt tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg
      421 gtatattatc tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg
      481 aagaggcacc tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg
      541 cggggatctt atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata
      601 ttactatcaa aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga
      661 ttccaggtgc agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg
      721 taaacgctgc cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc
      781 ttggtgtttt actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca
      841 tgagtattgt aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa
      901 aggcattggc agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt
      961 atcaatggac ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta
     1021 tattcttctg taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta
     1081 tcttcaatat caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt
     1141 tcatcccgtt caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat
     1201 tagaaatggt caatagattt actgttttgc caacatggac attaccagct ccaatcggcg
     1261 gttatctttc agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga
     1321 tagcatttat tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg
     1381 aacaaagtcg tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga
     1441 aacaatgtat tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct
     1501 tatattgaat atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc
     1561 atcatttttg atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc
     1621 atgggggaca tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag
     1681 atggtttgtc acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc
     1741 ttcaagaaat aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct
     1801 tatttttcgt ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg
     1861 gaatatggga ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg
     1921 ttttaactgt tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt
     1981 agaattagta gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac
     2041 catatagtag gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat
     2101 tcaaatcatg aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa
     2161 cgtgactttt agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata
     2221 tgtggggatt gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca
     2281 acagaagagg gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa
     2341 ttgttgaggg gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca
     2401 cattttaaga aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga
     2461 tgatccaacc agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct
     2521 atgctcattt ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca
     2581 ccgtaattta agtaactcta acttttcaat ccactattta ctttttttgt attttcactg
     2641 tctgggtctc gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg
     2701 aaactatatt gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc
     2761 atttattgga aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac
     2821 ttcgcgcaga gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt
     2881 tccgtatttt acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat
     2941 aaacggtttg ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga
     3001 aaaatcaatg aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc
     3061 tgagtcagat tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg
     3121 ttgtttttgt aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat
     3181 tgcgaaatag ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc
     3241 ttcaggcaag gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg
     3301 cagctgttct tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat
     3361 catttgtaaa cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc
     3421 attggggatc catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct
     3481 taaagggccg aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg
     3541 ttcactcaaa gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa
     3601 aaaaattgtc acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact
     3661 ctgttcacta gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg
     3721 caactggtca aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa
     3781 atgatactca aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa
     3841 tacacagtaa taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc
     3901 taaattgaat actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg
     3961 agtctatccc ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt
     4021 atattttgaa tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta
     4081 atcaagttga aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg
     4141 ttgtgccaga taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa
     4201 taaaataatt atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa
     4261 tagttatgtt taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat
     4321 aactgtaaac gataattatt cagataacac aaaaagaaat caaggagcag atacgccagt
     4381 tgtttaattt gaatattatt ttaaaattaa aatttttatt ctgttctaaa catttttggt
     4441 tgtttagaac ttttttttgt gtatgatata aaggagaaat aattttttaa agggaaggaa
     4501 attgttttga ttaaagtaga tggagcatta ctaaaagaat accgaaagaa aaaggggtta
     4561 acacaagtac aattagcaga aggcatttgt gaacaagcaa ctgtcagcaa tatcgaaaac
     4621 aagaatatgt cgccaggcat caatatctta agtgcgcttt gcaaaaagct gaatgtcgag
     4681 atctccgata tattagtaga aacagaagaa gacaaaatca ggaaaaaaat aaatgagatt
     4741 caatcattga attctgtatc aaaataccaa gaatcttata aaattttaca aacgatcgat
     4801 caggaaaaag taagtaattc tgatctttta aatcaaattt tattttatca aggactgctt
     4861 gatcatcttt atgcgaaaaa accagatgca gcattatttt attttaatca agtgttggaa
     4921 caaacgaccg aaacagatat ccatcactta caagcagctg caaacgtggc aatgatctac
     4981 ttgtcaaaag gagaattaga ttttgcgaaa gtttatgtgg agcgtacact taaaatattg
     5041 agtgagactg attttgataa tctgatggtc tgcattgtat attatgatat cgccacctat
     5101 taccgtaaaa tcgaggatta tgataaagca atccaattat gtgagaaggg tattgagtat
     5161 aataagaaac ataaatcgac ttatgcattg gaatacttgc tttatgaaat tgcttcttgc
     5221 cataagcaat taggagaaga tgattattta gaaagatata tggacgcaaa aaaaatagcg
     5281 cgtttcaacg gaaatgatta cgcggtaaag gtgattgaaa acgacctgaa ataaagtggt
     5341 tgtgaaatca gtgttctgat ttgaataccg tttattcgaa aaaaggaggt gtgacttttg
     5401 tcatacctcc ttttttcgat attgtttgat tcctatttag gcaacatata acggttataa
     5461 aagaagcaat agattatgat ggtatcgatt ggatggatac atcttgggat tcctttatca
     5521 atttgagcgt agcgtgtttt tcatttatcg ataaatttgt tattcatttt tttgataatc
     5581 ctatttacat atttacgtcc tggttcttaa aatgaatcta cttgaatggt ttttatcccc
     5641 gtatttctga tctcctctaa tactttttca gaacaaaatt tatcagtgat caaataatca
     5701 aagactgaaa tatcagcaat cttgaacttt gatgtcagtc cgatttttcg atgatcagcg
     5761 actaaaatct tacatttgat tgtcttgttg accatcattt cgttgatcgt tgattcattt
     5821 aatatctttg ttgtgacccc atgtactaca tcgactccgc tacagccaat gatacaaatg
     5881 tctgcattga ctgaagaaat cgtatttaat gcgatgtcgc ccactagtgc ttcttttgga
     5941 aagcgcatct ctccaccagt taaaatataa ctacaattat ctttgtgcgg agagtaattg
     6001 attttcacgt tgtttgtaat gactgtcaaa tggttagatt gaagaaaatc cagtgttttc
     6061 aacgcagtcg agctagaatt gataaacagc gtatcataat cgttaatgta tttactagct
     6121 tctctggcaa tcgcttgttt aatcctttca ggaccagatt gatcaaaatc agtctggttt
     6181 ttgtaattat aagaacaata accgtaattg cgtatgatct cgtttttttc ttctaataaa
     6241 tttaaatctc ttcgtatcgt actaatagat acatcaaggg attcagctaa ttcgaaagtt
     6301 gatagttgtt cagtctgctg aagcagttcc aaaatttctt tttgacgttt ttcaatattg
     6361 acaagtgagt ttttcatgac ttttgcctcc tgagggacat cttgatccat ggatttattt
     6421 gactagttgt tcatttaaac attcatatct gtctttatta tactgaaaca atcattgatt
     6481 actgaaagaa aatcatataa aaaacaaacg gtcatataaa agggttttca tggatgctga
     6541 tacacattaa tatttacttg taagttggaa caacgatatg gggggagcaa gcaaatgagt
     6601 aaaactgtca atgaagtaac aaaagaaaag tggttagtag aaacatttcc tgagtggggc
     6661 acatacttga atgaagaaat cgaaacagaa gtagtggatc ctggaacttt tgctatgtgg
     6721 tggctgggct gtacgggaat ttggctgaaa agtcatgaag gaacgaatat tttatgtgat
     6781 ctatggtgtg ggacgggaaa acgtagtcat gggaatggca tgatgaaaaa aggccatcag
     6841 atgatgcgta tgagtggcgt tcaaaatatg cagcctaatt tacgtactca gcctttcgta
     6901 atcgatcctt ttgcgataaa aaatgtagac gctttaatcg ttacacatat ccattccgat
     6961 catctagata tccatacagc cgcagcagtt catcaaaatt gtcctgaagc aacattcatt
     7021 gggccaaaag aagtggttcg cacgtggcaa aaatggggaa ttcctgaaga aaaaacgatt
     7081 acagtaaagc caggtgatgc agtcaaaatc aaagacatca atgtagtagc tttagaagcg
     7141 tttgatcgga cagcgttggt cacttgtgaa gatccagaag aggtattaaa aggaaaaatg
     7201 cctcaggaga tggatcaaat tgcagtaaac tatttattcg aaacatcagg agggaatctt
     7261 tatcatgcag gagattcaca ttactccaat ttatttgcca aacatggtaa tgaacacaaa
     7321 atcgatgtct gtttaggggc ctatggtgaa aacccacgag ggataacaga caaagttact
     7381 tctgttgata tgctgcgcat ggcggagtca ttgaatgcaa aagtagtgat tcctgtccac
     7441 tacgatattt ggtctaattt tatggctgat ccaaaagaga tcactgaaat ttggaaattc
     7501 aaaaaggatc gcctgaagta tggatttaaa ccttatatct ggcaagtagg cgggaaattc
     7561 atctatccga caaacaaaga tgatcttgag tttaattttt atcgaggatt tgaagatgtc
     7621 ttctcgattg aaaatgatac accgtttccg tcgtttttat aagaaagaag gggagtttct
     7681 tgctgaaata cttttttgac catcaattga ttcattaccc agataaagaa atcacggatt
     7741 ggcgtgaagc tatcgccgaa agctgtcttg tattgatcga taaggaaatt attgatgaaa
     7801 cgtatgtgga ggagatcatc cattgtgtag aaaaatacgg tccttatatt gtgatcgcac
     7861 ctcaagtagc aatgccgcat tcttcagaaa aaagtgatgg tgtttttgga acagctattt
     7921 catttaccaa gttgaaacag ccaatccgtt ttgaaagtga aggggagaat gaggagaaaa
     7981 cggctagttt gttttttaca ctcgcagcaa aagatccaga agaacatcta gaaaatatcc
     8041 aaaaattatc cgaattatta atgacagatg gattggttga gcgtctcatc tctacgaact
     8101 ccatcgatga ttaccaagtt gtgatggcag aatataatta attgatagag gagaaaaaag
     8161 atgggggaat ttttgttaag catttggacg tattttgcaa caaatatctt gacgcagcca
     8221 gcatacctga ttggttttat cgtgttatta ggctatattt tgctgaaacg cccgcttcat
     8281 gaatgcatcg ctggattctt aaaggcaacg gtcggttact ttattttgac tgttggctct
     8341 ggtggtttgg tcaataattt tcggccaatc ttggttggac taaaggaacg gttcaatttg
     8401 gatgccatgg ttacagatcc ttattttgga caaaatgctg tggatgctgg attaatggag
     8461 acatttggcc gtacctttgg agatgtgatg attttattac tgattgcatt tgttttaaat
     8521 atcttattag tccgttttca gaaatatacg aaactgcgtt ccgttttcac taccggaaac
     8581 gttcaggttc aacaagcagc taccgctttt tggattttac tgttttgctt tcctgatcta
     8641 ggtaggatac aagcattgat ttttatggga ctgattttag gttgttattg gacagtagct
     8701 tctaacttaa cagtaggaat cacgcaggaa ttgacagaag gtgccggatt tgcagtggca
     8761 catcagcaaa tgttcggaat ctttatattt gcaaaattag ctgagtggat gaaaaagcgg
     8821 gatgaaaaga aaaatagatc aatcaagcaa gacaaaaaaa ttgaagatat caaattacca
     8881 ggattcttgt cgattttcaa tgagaatatg gttgctactt cgttactcat gctatttttc
     8941 tttgggatca ttctgattgt actaggcaaa gattatttga ttcaagcgca attcatgcaa
     9001 gaagggcaga gtttcttatt ctacattatg acgacttctt taaattttgc ggtatatctg
     9061 gcgatcttgc aattaggtgt tcggacattc gtcgatgaac taactcaatc tttccaagga
     9121 atctcaaaca cgattttacc aggagctgtt ccaggaattg acgtagctgc tacttttggc
     9181 tttggttcgc caaatgcggt aacgattggg ttcttgtttg gtgctttggg acagtttttg
     9241 atgattggtt tactgatttt attgaaatca ccaacgatcg tcatagccgg tttcattccg
     9301 ttgttctttg ataatgccgt tatagcagtt tatgccaata accgaggtgg attcaaagcc
     9361 gcttgcctgt tcccgtttct ttcaggaatc atacaagtag gcggttcagc attattcgct
     9421 acatggattg ggatgtcaac atacggtggt tatatcggga tgttcgactg ggcaacagct
     9481 tggccattgt ttacagtaat catgaaagtc ctaggttatg ctggaattgc cgtagttgtc
     9541 attggtttat tggctattcc gcagttacaa tatcggaaaa atcctgatgg ttattttttg
     9601 attgtagaag actatgacac atatgcagaa aagcagagag tataaaagaa aatctgatta
     9661 gacaacactc ataatggagg gattaagatg agaattttag tatcgtgtgc aaatggatca
     9721 ggaacaagct tgatgatgat gagaagtgtg gaaaaagcaa tgaaagaact aaacgtacca
     9781 attaccaaaa tccatcattg tgccatttca gagggaaaaa gttcagctgg ccaatacgat
     9841 gtagttttta cgccagtcaa ttttttgaat atgtttactc aggcagagaa aaaaggagtt
     9901 acagttatcg gtatcaaaaa tgtgatgtca gtcaaagaaa tcagtgaaaa atttgctgaa
     9961 acaaagtggt accaaaaaaa caagtaaagg aaggaactaa gatgagcagg ccgaatttgc
    10021 aaattgcgtt agatcacaat gacttagaac atgcattagg agatgttatg aaagttggtg
    10081 atgtagtaga tattattgaa gtgggcacaa ttctttgctt acaggaagga caaaaagcga
    10141 ttcgctgtat tcgcagtatg ttcccagata aaaaactagt cgctgatacg aaatgtgcag
    10201 acgctggagg aacagtggcg agtaatgttg caaaagcagg agcagactgg atgacagtca
    10261 tttgttgtgc aacgattcca actatggagg cagcacaaaa agaaataggt gaattgcaag
    10321 tagaactcta cggcaattgg acttttgaac aagccatgga ttggcataac atcggtatcc
    10381 ggcaggtaat ctaccatcaa agccgtgatg cgctgttatc tggtgaaact tggggtgaaa
    10441 aagatttatc gaagatcaaa aaactgattg aactaggctt taatgtttct gtaactggtg
    10501 gattaaatcc acacacatta cacttatttg aagggatcga tgtgtatacc tttatcactg
    10561 gtcgtggtat tacagcagca aatgatccga tgaaggccgc acaaaacttc aaagatgaga
    10621 tcattcgtat ctgggggtaa ggaaatggct caaattggga tttatgaaaa agcgttaccc
    10681 aaagatattt catggaaaga acgtttttcc cttgtaaaag aaatggactt cgattttatc
    10741 gagatgtcga ttgatgagac ggatgagcgg ttagcacgat tagattggtc tgaagaaaag
    10801 atagctgagc tgagagaaga gatgttcagc tccggtgtac gtatccattc gatttgttta
    10861 agtgcgcatc ggcgatttcc gtttggttct gctgatccag aaaagaaaaa agcagcgaaa
    10921 caactaatga aaaaagcaat ccatttggcg cataatctaa gcgtcaaagt aatccaaata
    10981 gctggatacg atgtttatta tgaagagaaa tcaatgaatt ccagagaaga ctttatcgaa
    11041 ggaataaaag aaagcgtaaa ggaagcttct aaatatggca tcattctttc cgtggaaatc
    11101 atggatgatc catttatgaa ctccatttca aaatttttag aaatcaaaga acagattcat
    11161 tcaccttttc tacaagtata tccagaccta ggcaatttgt ccgcatggcc ggaaaataat
    11221 ccggctcgag aacttgagaa agggatcgat tatatcactg ctatccactt aaaagatact
    11281 taccctgtaa caaaagaata ttcaggccag tttcgtgatg ttccttttgg tagcggttgt
    11341 gttgattttc ttggattatt aagacatttg aaacgtctag attatgatgg aacatttctg
    11401 attgaaatgt ggagtgaaaa aagccaagac tttcgaagag aaatccagca ggcaaaagac
    11461 tacttaagtt ctaaattgaa ggaggctggc tatgatgtct tgtgaagaaa aaattgcagc
    11521 aatgaagcaa cgtgtccttg aagcaaatct ggcattgccg cgatacggat tagtcaaatt
    11581 gacttggggc aatgtgagtg aagtggaccg tgagctagga gtcatcgtca ttaagccaag
    11641 cggtgtcaga tatgaatgca tgcaagcaga tcaaatggtg gtaacagatt tgtcgggaaa
    11701 tataatagaa gaagatagct taaaaccatc ttcagatctc cccacacatg ttgttttgta
    11761 tcaaactttt gaagatataa ctgctattac tcatactcat tccacgcact ccgtcatgtg
    11821 ggcgcaagca ggacgcgatt tgccagctta tggcacgact catgctgatg ctttttatgg
    11881 taaggttcct tgtacacgcc aattaacgaa agaagaagta agagaagctt acgaagttca
    11941 tacagggaat gtgattgtcg agacgtttaa ggaaagaaaa ttagatccaa acgaagtccc
    12001 tggagtactt gtctatggac atggaccgtt cacatggggg gattcaccta tgaaagcagt
    12061 agaaaacagt ttgatcttag atgaaatttg tctgatggcc aaagaaaacg agctgatcaa
    12121 ccccaatata tgtgaaatcc cgcaatattt actagataaa cattattttc gcaaacatgg
    12181 aaagtatgca tactacgggc aatagagatg ctagggtaag ggtagaaaat taaaagataa
    12241 cacttgatag agagaaacag aggttgtgaa gaaaatttat cacaaccttt ttatttatca
    12301 aaaagtatgc attctaaaag gaaagaggaa ttttaaggaa tcgagagaat ctttacagga
    12361 gagttatatg tgatagatta aatagtcaaa gaactgtatt attagaggga gaattgtata
    12421 agaaaggaag aaaaaaatga atattgattc ccttatgatc gatgagattt ctgaattata
    12481 taagagcgtt ggttggacga attacacaaa agacactgca aggttagcta gagcatttga
    12541 acaatctgaa tctctgataa agcgtaatgg ggaagggaag attatcggaa tcgttcgttg
    12601 gatcactgat tttgcgacga tcgcatttat tcaagatatc ctcattcatc cgaggtatca
    12661 gcgccaagga ataggcaaag tgcttttgaa tgaagctttg gagaagatca ctagttatgg
    12721 accagtccaa atcgaattat tgacagatga tactgagaaa acaaaaaaat tttatgaatc
    12781 agtaggattt gttcaagtaa aggaaatggg tggtatttct tatgtcatag atactagaag
    12841 aaaaaaggtg ctttttggta atgacaaata acatataata acgtacaaaa gaaaaaattc
    12901 tagcttagtt ctttagaaaa acaagaggat agacttagta tcactcaatc tatcctccaa
    12961 tgctcagcta ttttattttt ttactcacct ctttatcaaa tacaattttt tgcaaattcg
    13021 aactaatata aaaaatgaac acatgatttt t
//
LOCUS       DAYYJC010000075        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000075 DAYYJC010000000
VERSION     DAYYJC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.077"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA9N_002054"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA9N_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9085501.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA9N_002055"
     CDS             complement(322..891)
                     /locus_tag="ACHA9N_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9085502.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA9N_002056"
     CDS             1077..1979
                     /locus_tag="ACHA9N_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9085503.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA9N_002057"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA9N_002057"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9085504.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA9N_002058"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA9N_002058"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9085505.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA9N_002059"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA9N_002059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9085506.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA9N_002060"
     CDS             8547..8732
                     /locus_tag="ACHA9N_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9085507.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA9N_002061"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA9N_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9085508.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA9N_002062"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA9N_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9085509.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA9N_002063"
     CDS             11889..12698
                     /locus_tag="ACHA9N_002063"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9085510.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAYYJC010000076        12306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000076 DAYYJC010000000
VERSION     DAYYJC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.078"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..693
                     /locus_tag="ACHA9N_002064"
     CDS             286..693
                     /locus_tag="ACHA9N_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085511.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="ACHA9N_002065"
     CDS             707..1108
                     /locus_tag="ACHA9N_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085512.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="ACHA9N_002066"
     CDS             1110..1481
                     /locus_tag="ACHA9N_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085513.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1517..1816)
                     /locus_tag="ACHA9N_002067"
     CDS             complement(1517..1816)
                     /locus_tag="ACHA9N_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9085514.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1822..2052)
                     /locus_tag="ACHA9N_002068"
     CDS             complement(1822..2052)
                     /locus_tag="ACHA9N_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085515.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2669..3331
                     /locus_tag="ACHA9N_002069"
     CDS             2669..3331
                     /locus_tag="ACHA9N_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085516.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3410..6635
                     /gene="fss3"
                     /locus_tag="ACHA9N_002070"
                     /pseudo
     CDS             3410..6635
                     /gene="fss3"
                     /locus_tag="ACHA9N_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6749..7063
                     /locus_tag="ACHA9N_002071"
     CDS             6749..7063
                     /locus_tag="ACHA9N_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9085517.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7076..7450
                     /locus_tag="ACHA9N_002072"
     CDS             7076..7450
                     /locus_tag="ACHA9N_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9085518.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7460..7804
                     /locus_tag="ACHA9N_002073"
     CDS             7460..7804
                     /locus_tag="ACHA9N_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9085519.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7875..9227
                     /locus_tag="ACHA9N_002074"
     CDS             7875..9227
                     /locus_tag="ACHA9N_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9085520.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9287..10429
                     /locus_tag="ACHA9N_002075"
     CDS             9287..10429
                     /locus_tag="ACHA9N_002075"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFM9085521.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10454..10708
                     /locus_tag="ACHA9N_002076"
     CDS             10454..10708
                     /locus_tag="ACHA9N_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085522.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10964..12148
                     /gene="mobT"
                     /locus_tag="ACHA9N_002077"
     CDS             10964..12148
                     /gene="mobT"
                     /locus_tag="ACHA9N_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9085523.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12145..12282
                     /locus_tag="ACHA9N_002078"
     CDS             12145..12282
                     /locus_tag="ACHA9N_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9085524.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         4168 a         2017 c         2646 g         3475 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atgaatttca agcgagtttg
     1501 tttgttattt ttttatttaa tctttgtatc tttctggatc aacgaaagta tactttatat
     1561 agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa
     1621 agtatacacc cttcttcatt ctagttttcg cagcaggaat tttgaaatag ttcttattag
     1681 aatagtagag attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc
     1741 atactttata aaaatctttg ataaatctat tcgtaggatc gaatttaaac cataattgtg
     1801 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
     1861 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
     1921 ttgtttattt tcggaaaaag ctaatatagc taaaattgaa acatccatct ggaatttatc
     1981 ttttttgctg ctttgctcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
     2041 gtcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct cgttgtaaag
     2101 cgatatttga ggggcaaaaa gggggcaaaa atacgtttat ctttgatata tctcagattg
     2161 aacatagtgt ttattatatg tcggtattgt tgatttatag ctgttttgag ggtattagat
     2221 acataataga atatagtggt tttgtttacg tgcattacac ggaaaagctg ctcgtatcaa
     2281 agaaatcaga agataaacac ggaaaacttc cttggaaatc tgaaatttgt gaaaagtcct
     2341 tgtatatcaa gggctttttc ttttttctct atcacagttt taagccattt ttggctgttt
     2401 tggaccattt ttcaatcata ttcccttaaa aattgtccta gattgtccga cattgactat
     2461 cagtgtcgtc aaaatagggc aaaatttagt agtcagcttg acgacaaata taatacgaaa
     2521 aataccaaaa catttaccga atttagcaac ttttgaaccc aagtttctca aagggtcagc
     2581 tattagcaaa aaagacaaaa catgtacttt tctactaaaa agttgatgtg ataggcttct
     2641 atctaaataa ctattaggag tttttatcgt gaagcaaaat gatggacgta tcatttggaa
     2701 aaatcaaagt gagttaaaac tgattttaac aattaatgaa ttcatcgaaa aacacggaat
     2761 tacatctagc cgtcaatacc agaaaaagct atcagaaaat ccaaattctg caccaagtat
     2821 gtggttcatt aacaaaaaat atggatcatg ggagaattta ttgattagta taggacgaga
     2881 aaatactggg tatggaaaat gggctagaat gtcagaacaa gaattattgg aaatagtaga
     2941 agcctttatt aagtgcgaga agataacctc tcaacgaatg tacgaacaaa aatcagtagg
     3001 gaagaatatt ccttcactta gtaccattaa aaagatgtta ggagatatac gtccactttt
     3061 taaagaaaaa aatgatggat ctcgatttac tgatttcgaa ctgctgttag aactaaaaaa
     3121 tgaaataatc cgattaaaac tacaggatga tttatcaatg acaaagtttc gaaaactagt
     3181 tcaatcccca aaactaccat cagttgatac aattatgaaa agaacgaaca aaaattggga
     3241 ggaattgatg gcagagattg gatttgatta tcgaagaatc aaaatttata agcagagaaa
     3301 caacctatcc aagacaaaga aaactaaata gtatcactct aaatcagacg ttcaaaatta
     3361 aaatttggac gtctttttta ttacccaaaa acgaaaggag atttatttta tgaaaaagcc
     3421 aagatttaag aattggcgtt tgctcgcaac actgagtcta ttatgccaaa caattggtgg
     3481 atcgcttggc ccaacgattg cctttgctga tgaaattact catccacaaa cggtaactgt
     3541 ggaattggat ttggctcacc agtatgctgt cgaaggtaca tttagcgatg gtcgtcccat
     3601 gtcagaagtt accgttccgc actatgcagt ttacaatggt gtgaagcaag atattttctg
     3661 tattgagcca ggggtgccga tttacaatga gttcactcct ggttatgaga aaaatccatt
     3721 gcctgatatg tcggagaaag cgaaattagt ttccgtttta tggaaggaag ctggtacgga
     3781 tgtggataca catattgtgg cccagaagat gatttggcaa gaagttaatg gctacgcttc
     3841 actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag tcaaaaatca
     3901 atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca gtgaaaaccg
     3961 tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg gagtttgatg
     4021 aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa ctagtgatta
     4081 ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca gctaatacag
     4141 gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg gcaattgata
     4201 agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg aagattaaaa
     4261 aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta gactttggaa
     4321 aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca ttggatgaga
     4381 ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct tatacgattg
     4441 acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg acttcaaaaa
     4501 atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca ggaactgatc
     4561 tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa gacagtccaa
     4621 ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa acaccaaaag
     4681 agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa gctagtaatg
     4741 gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat caaaccgtgg
     4801 ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa accactttga
     4861 caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt aaaggagctg
     4921 aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag gcttttaaac
     4981 cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact ttagatgaaa
     5041 agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
     5101 cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa aaggtggatg
     5161 ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa gtcattcgct
     5221 ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa actggcttca
     5281 atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca ggtgccgaag
     5341 atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc aagtttgaaa
     5401 atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga tttcaaaaaa
     5461 ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac actaagagtg
     5521 aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg gtgaccgttc
     5581 cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg atgctctatg
     5641 atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac ggaggcaaag
     5701 aattgacaga ccttgattct accgagctag ttgataaatt gagctataac ttgcatgaaa
     5761 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca aaagctgccc
     5821 aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca acgttggcga
     5881 ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa caagttttgg
     5941 ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat gaagcaggtg
     6001 atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc aattgtacgg
     6061 tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg caaaccttta
     6121 ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat gtactggatg
     6181 gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat acgaccaaag
     6241 aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc actaaaaccg
     6301 tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt accttcaaag
     6361 aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat ctaaaagaaa
     6421 aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca gaacaaccgg
     6481 aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag acattcccac
     6541 aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg atctttgcga
     6601 ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa caactaaata
     6661 ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt tttgtatcca
     6721 aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc cagatatggc
     6781 cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg aaggatatgg
     6841 gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc aacgagccga
     6901 tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc aagatcaacc
     6961 actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt
     7021 taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa agaagatgag
     7081 attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac gattttccgc
     7141 attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg aggtaaaaga
     7201 acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta gtatcccagc
     7261 aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca atccaatcat
     7321 ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt tcattaaggc
     7381 cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc aaaatgaagg
     7441 aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt gtctcgatca
     7501 atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct atgatgaaaa
     7561 aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgccg aagaagattt
     7621 tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg aagtaaatga
     7681 aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa tgccggatgc
     7741 cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag cagtaaatga
     7801 ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt cgattggagg
     7861 tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag cacttggttt
     7921 atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt
     7981 ttcaagagct tagacaattt gatatttcta agctatcact ttctactata gaaatcgttc
     8041 gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga agcacgatgt
     8101 tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga caaaagctag
     8161 cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt gaaggatttt
     8221 tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc aaaatctatt
     8281 accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga aaatatcaag
     8341 atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa ctagtagaaa
     8401 aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt gccaatcgaa
     8461 ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa aatcaagtat
     8521 gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt gggaagactt
     8581 actttttact cactatcatc gaggctttat taaaatctga tgccgagctt tttgttcttg
     8641 atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc tattcccaaa
     8701 aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact cgtagcaagg
     8761 caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt ggacttccac
     8821 caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg acgaaggaaa
     8881 gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa tctggtttct
     8941 ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg attcgcgatc
     9001 aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc atgatgtttg
     9061 gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat gtagatacgg
     9121 gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat gatttcttaa
     9181 gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat aatagtatgt
     9241 agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga catttttaga
     9301 tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc accagtgtat
     9361 cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc atgatacaag
     9421 taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc aatctctcgg
     9481 accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg cgggaaagcg
     9541 gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcgccgctat
     9601 tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc acgaaggagg
     9661 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg tggaatggca
     9721 agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc ttatcggaca
     9781 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc caacatctga
     9841 tcaaaacatc agaaacttat taaatatcaa cccttccaat cgaggaatgg ggggacaagt
     9901 ctatgggtca gatgacgtag cgcctacctt gaccggtgat gaaggaatca aaatcgcatt
     9961 gccggtagat gatggaattt ctgtagcggg tatgttactg ggaaattttg aacaaggcaa
    10021 tcgggtttat gagataactg gaacagcgcc tactttgtca acgaaacaag gcggaacgaa
    10081 gatcatgctt cggaaaaacg aaacagaata tcaagaggtg aagccaggga acagtgtgaa
    10141 cctggctttt cctaattcca caagtcgtcg aggccgtctg ggaaaacaaa gtgtgcatac
    10201 cttattaaca ggagaccaac aagcagtcgt cacggatcag tatcaaattc gaaagttaac
    10261 acctagggag tgctggcggc ttcagggctt tccggactgg gcttttgatc gagcttctca
    10321 agtcaattca gatagtcagt tgtataagca ggctggaaat tctgtaacag ttccggtcat
    10381 ttttgatatt gctagaagat taaaggaggt aaaaacagat gatttatgat gagttgattg
    10441 gggaatctac tgggtgattg gcaagattca gtcagatccg gaattggaag aagaactgta
    10501 ccggctcaat tttgatattc gcaaaaacgg ggtgaaagtc cctgatgatc cgtatgtaat
    10561 tgatgaggaa acggatgctc gaatggagct gaatcaagtc atcagtgaat tggaacgaat
    10621 tgcggacttt gctaaagaac ctgatatccg ccaatacttg tttgagataa aagcagagtt
    10681 ggaaattgag ggtattaccg cggagtaacg gccaaataaa ccactgatag gcactgaccg
    10741 cgggggattt gcgaagcaga aatcctccgt ggtgctgtgc ctgtttatgg acggcgttag
    10801 ccggccatga tgtctcaccc cccgtatcta acaggggggt acaaaaacac aaaaaataag
    10861 caagcaacga taaggaatgg ctgtcctatc aaggataata agcaatttgt gtagatgtta
    10921 actgatttga aaaagttaac accttaaatt tggaggtgag aacttggcac aaaggaattt
    10981 agattatcgg ctattaaaag accggcgtaa tgaatatggc gtttcacaaa ataaattagc
    11041 tacgacttgt ggacttagtc gcccgtactt aaatcagata gaaaatggtg gcgtaaccgc
    11101 atcgacaaaa accatgagga aaatttttga ccaactggaa agtttcaatc ccgagttacc
    11161 tttaacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg cgaggaaaat
    11221 cattcaagag attctccatt tgaaatttga ttatatgctc cacgaagatt acgcctttta
    11281 ctcctatcaa gaacaatatg ttatgggaga tattgtggtg atgttgtcac atgaagaaga
    11341 taaaggcgtc cttttagaat taaagggtcg tggttgtcgg cagtttgaaa cttttttact
    11401 cgcccaaaag cgtagctggt atgacttttt cgaagattgt ctaaaagccg gtggcgtgat
    11461 gaaacgctta gacctggcaa tcaatgatcg agttggcctg ttagatattc ctgatttaac
    11521 gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt accttcaaaa gctatcgttc
    11581 cggggaactg ttgaaacctg atgataaaga tggtatgggc aatactttgt atattggcag
    11641 tcttaagagt gaggtctatt tctgtttgta cgaaaaagac tatgagcaat atatcaagtt
    11701 agggattcct ttagaccaga ccgaaacgaa aaatcgtttt gaaatccgac tgaaaaatga
    11761 tcgagcttat catgcaattc aagatttgtt aaaaggtcgc agtattgaaa gtaccacatt
    11821 ttccattatt aaccggtact tgcgatttgc ggataaggtg gaaggcaaaa ggcgaactaa
    11881 ctggcccttg aatgaacaat gggggcgctt cattggacgg aatcagaagg aaattcaatt
    11941 aacttctgaa ccgaagcctt ataccattga acgaacgttg aactggcttg gacgacaagt
    12001 cgcccctacg tggaagatgg ccaaggagtt ggatcggttg aatcagacaa cgtacattca
    12061 ggatatggta aataatactc gactatcaga ccggcacaaa aagattttgg agcaacaaag
    12121 tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tgatggaaat tgtgaaagat
    12181 attttattgg tgtttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgcatgca
    12241 agtgcagcgg cggaccgagc aatggaaaag aaaggaaagt gatgaaatga atttcggtca
    12301 aaattt
//
LOCUS       DAYYJC010000077        12242 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000077 DAYYJC010000000
VERSION     DAYYJC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.079"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..602)
                     /gene="frr"
                     /locus_tag="ACHA9N_002079"
     CDS             complement(45..602)
                     /gene="frr"
                     /locus_tag="ACHA9N_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9085525.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(605..1327)
                     /gene="pyrH"
                     /locus_tag="ACHA9N_002080"
     CDS             complement(605..1327)
                     /gene="pyrH"
                     /locus_tag="ACHA9N_002080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9085526.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1463..2344)
                     /gene="tsf"
                     /locus_tag="ACHA9N_002081"
     CDS             complement(1463..2344)
                     /gene="tsf"
                     /locus_tag="ACHA9N_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9085527.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2443..3225)
                     /gene="rpsB"
                     /locus_tag="ACHA9N_002082"
     CDS             complement(2443..3225)
                     /gene="rpsB"
                     /locus_tag="ACHA9N_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9085528.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3584..4063
                     /locus_tag="ACHA9N_002083"
     CDS             3584..4063
                     /locus_tag="ACHA9N_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9085529.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4280..5371
                     /gene="phnW"
                     /locus_tag="ACHA9N_002084"
     CDS             4280..5371
                     /gene="phnW"
                     /locus_tag="ACHA9N_002084"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFM9085530.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5364..5492
                     /locus_tag="ACHA9N_002085"
     CDS             5364..5492
                     /locus_tag="ACHA9N_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9085531.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5496..6041
                     /locus_tag="ACHA9N_002086"
     CDS             5496..6041
                     /locus_tag="ACHA9N_002086"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFM9085532.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6495..8186)
                     /gene="argS"
                     /locus_tag="ACHA9N_002087"
     CDS             complement(6495..8186)
                     /gene="argS"
                     /locus_tag="ACHA9N_002087"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9085533.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8606..9553)
                     /gene="arcC"
                     /locus_tag="ACHA9N_002088"
     CDS             complement(8606..9553)
                     /gene="arcC"
                     /locus_tag="ACHA9N_002088"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9085534.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9668..10687)
                     /gene="argF"
                     /locus_tag="ACHA9N_002089"
     CDS             complement(9668..10687)
                     /gene="argF"
                     /locus_tag="ACHA9N_002089"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9085535.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10778..12007)
                     /gene="arcA"
                     /locus_tag="ACHA9N_002090"
     CDS             complement(10778..12007)
                     /gene="arcA"
                     /locus_tag="ACHA9N_002090"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9085536.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3357 a         2533 c         2205 g         4147 t
ORIGIN      
        1 acagaggaag cggaatgatc cgcccctctg tttttatgat tttattaaac ttccaataat
       61 tctttttctt tatcagcagc gatcttatct acttctttga tgctgttatc tgtcaatgtt
      121 tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt
      181 tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt tgcattttct
      241 gcttcttttt taacatcttt agctaattct tttctgcgtt cttctgtcag ttgcgggata
      301 actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact tgccataata
      361 gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct
      421 tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta gtagttcaca
      481 gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc aagttcacgt
      541 tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat tgcatctgcc
      601 atttttattt cccccttaca gttgttccga tattttcgcc taagattgca cggcgaatat
      661 ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg cttagtgaac
      721 ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga gtcagttctt
      781 caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg tctacattgt
      841 ttttagccat caggataaca tctgcgccga tttctgccgc gcgtaatgct gctgttgtgt
      901 ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt cctttttcta
      961 aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag
     1021 tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct aaggcattca
     1081 taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc atttgtgctc
     1141 cgatttgtcc gcgccagata tttccgccgc ccacaacgat tgccatttca acgcccagct
     1201 cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg attccaaatc
     1261 ctgcgtcccc agctaaagct tctccactca attttaagac tacacgctga tatttcggtg
     1321 tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta gatgatttcg
     1381 acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt
     1441 tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct tctacaaagt
     1501 tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg
     1561 cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg aatggttggt
     1621 ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga
     1681 tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt tcatgttcta
     1741 attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct gctacgtgca
     1801 tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc aatacggcaa
     1861 tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt tctacaactt
     1921 caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc aagtcagatt
     1981 caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct ggtttgtttt
     2041 cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt ttagaaacaa
     2101 agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca gcaactgaag
     2161 ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt cctttttcac
     2221 gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct tttttcgcat
     2281 ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct gtaacgtctg
     2341 ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca aaggagagtg
     2401 aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg ctgaagaatt
     2461 gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa
     2521 atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat cagccatttt
     2581 tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta ctacgtcgat
     2641 ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta atttgtgtgc
     2701 ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat ctgggattct
     2761 tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc
     2821 agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg cattgatttg
     2881 tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc cgcctaacca
     2941 acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg cttcttgtgc
     3001 ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc tcttcatgta
     3061 atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt agattccgtt
     3121 tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt ggtgaccaaa
     3181 gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg tttcctccga
     3241 tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt tcaagcacct
     3301 accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca gctattatac
     3361 agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt caatagttta
     3421 cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc tttattatcc
     3481 tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat ccttcttttt
     3541 tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca aagctcaaag
     3601 acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag aagaactatt
     3661 aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc gagatatccg
     3721 tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg tattgtttcg
     3781 cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc aaatgattga
     3841 agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata ccttgccaga
     3901 caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta ttgtcagtac
     3961 aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg caaaacgagt
     4021 agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc acgtattcct
     4081 aatatggaat gcgtgttttt ttattttcaa tattttcctt attgaataaa cctttacact
     4141 ctttacaaaa atacatcatt ttttattcaa atttttcaac accttgattg aatcacaata
     4201 atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa
     4261 agaaatgaaa ggaaatcata tgtataaatt attaacttct ggaccattga cgacttctga
     4321 aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat acaaagaaat
     4381 tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag gaatatacac
     4441 aacggtctta atgcaaggta gcagctcctt cggggtagaa tcggtattaa gttctgtgat
     4501 caggccagaa gatactgtac tgatttgttc aaacggcgca tatagacaac gaatgatcaa
     4561 catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcggaac atcaaatcct
     4621 ccaagctcat gtgattacac agctgattga agagaatccg tctatctccg cactagcaat
     4681 ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg ctgctgtcac
     4741 acaagaaaga gatcttttgt ttatagtgga cgcgatgtca agctttggtg ggataccgat
     4801 ttgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat gtattcaagg
     4861 tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctggaaaaac gaaaaaatgt
     4921 tagccgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa acgagaatgg
     4981 caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag cgctgaagga
     5041 actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca ataatcatgt
     5101 gctacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat atcaggggcc
     5161 attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc atttttatca
     5221 gtatttaaag caaaatggat ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt
     5281 tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat
     5341 caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa
     5401 ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag
     5461 gaatccaact gactgacaaa gaaatccgct aaccgatggg aaaattaaaa tgggatcata
     5521 tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac
     5581 tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag
     5641 atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa
     5701 acgctgggat caatattggt tccacgaatg gatttaatgc tcctatgatg acaatcgtag
     5761 cagaaaccgc aaaaaaagaa aggtattcac cagacttcat ggcaactttt gaagacgtcg
     5821 aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca
     5881 agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa
     5941 atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg
     6001 atgagtacca aaaattagag atggggaaaa agatcgttta aaacagtctg ttgctcttag
     6061 attccaacaa gccggcgctg atatttgtgt aaacaattta aaagagttaa cagaatacat
     6121 tttgtacgct gacatgaatc cagaatgact tttatacaaa ataagaggtt gtgaaaaagc
     6181 tgtcctgtat caagtaataa aaacagccaa gcaaaaatag ctcctcatat ttttcgtttg
     6241 gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaaggg
     6301 agctgtgaca cgacttttgt cacagtcccc ttatttgcaa acaaacagtg tttaagagac
     6361 aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca aaaaacgata
     6421 aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa cgctgatttc
     6481 acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg aaggtcttct
     6541 ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg ttcatcatca
     6601 gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt gattgcatgt
     6661 ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc agggtatttt
     6721 tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc tgctgtttct
     6781 gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt atattgcaca
     6841 taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc aaatgtattc
     6901 agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg tttagctaca
     6961 gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc agattggatt
     7021 gcttcattta atacttcttc taataagacg atctttcctt tacgagttga taatttttta
     7081 ccaccttgcg tgatcaaacc aaatggaata tggtgcatgt cgtcagacca gtcaaagccc
     7141 atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt ccctactaca
     7201 tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc taagtcacgt
     7261 gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag gtcataagca
     7321 gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc ttctaaaagt
     7381 ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga atcgaaacgt
     7441 acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg gaaccattgc
     7501 cataattgaa tagcctcttt atcgccttct tccagacgtt tgaaccaagc acgagcttct
     7561 tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacgtataa acgaagcaat
     7621 tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta agcgacgatc
     7681 agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg ctgataaccg
     7741 attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa atgtcccatt
     7801 gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc ttgattcccg
     7861 atgttgctat ccccataatg ttctttttct ttcactactg tttgcaaaac ttttttgctg
     7921 ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat tttttcaaag
     7981 ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc ttttcggtaa
     8041 acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga ttttggattt
     8101 tctagtaatt gctcgacttg ttccagtgtt aaatcatctt tgacagcgtc atagacagct
     8161 tttgcgacaa gatctttatt attcatcttt attcctccta aagtgttccc ttgattgtta
     8221 aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga cgagatttcc
     8281 cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc ggtagacgac
     8341 cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca ttcggcttcc
     8401 accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca acaaatctta
     8461 gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag tcgtttcata
     8521 aaaaaacaat ccagatggcc taaaggagaa gaaataccac ctggatagct ataaatcatt
     8581 tcgtaaatac gaaagactta acaaattaat cagctacgat aatcgttcct tcttcagaag
     8641 ctaaaagatt ctcgatattt tctaaagaag tgatgattgc cttagcgttt ggttttgctt
     8701 caacaaatgc aatagctgct tctactttcg gaagcatgct tcctggagcg aattgctttt
     8761 catcaatgta ttgtttcatt tctgaaacag taacagtttc taatttcttt tgatctggtt
     8821 tttggtaatt gacatacaca ttgtctactc ctgtcaatac gatcagcaaa tccgcatcga
     8881 tgatttcggc taatttttct gaagcaaaat ctttatcgat tacggcttct cgtccttcta
     8941 atccagtagc tgtttctaca acaggaatcc caccgccacc aacagagact gtgataactc
     9001 cttgatcgac cagcgtttcg atgacacgtg cttctttaat cgagatgggt ttaggggaag
     9061 cgactacttt tctccatcca cgtccagcat cttctttgaa tgtcacagtg gagtcagcat
     9121 tcatttgttc tttggcttct tcttccgtgt agaatggacc gactggttta cttggatttt
     9181 tgaatgaagg atcattttca tccacaataa cttgagtgac taaagaaacc acatctttat
     9241 cgatcccttt ttccttcagt acttcgccca tggcgttttg taaccagtaa ccgatagatc
     9301 cttctgtcat tgcaacacat gtatctaaag gcattgccgg tgttttctca gaatcagcag
     9361 cttgctgctg aatcaatagg ttaccgactt gcggaccatt tccgtgggag atgatcaatt
     9421 catctccttg ttcgataaat ttcactaaat actttgctgt ttccattaac gcttcttgtt
     9481 gggcttttgc acttgcatcc gtagacaaga tggcattgcc ccctaaagcg acaactactt
     9541 ttcgtttggc catttcattc gtcctttctt cagttctacc gaattttcta gataacaaaa
     9601 cgagccagcg ttttgtgatt ttccctcaat gctgactcgt tttttcgtga gatgaatgtg
     9661 gttatgctta aacgcgaggg ataaacaagt tacctaatgt tgctgccatg atcgctttga
     9721 ttgagtgcat acgattttct gcttggtcaa attgacgagc atatttgctg cggaagactt
     9781 catctgttac ttccatttca gtgatgccaa agcgttcttt catttgttca ccgtaaacag
     9841 tgtttgtatc atggaaagct ggcaagcagt gcaagaagat caaacgatct gtgttgtgtg
     9901 ttttttctac catttccata ttgatttggt aaggtttcaa caatttgata cgttcttcga
     9961 atttatcttc ttcacccatt gatacccata catcagaata aaggacgtct gctccgtcta
    10021 cacctttatc aacatcgtcg gtaatcatca attgtgcacc ggatttttca gcaaagcctt
    10081 ctgccatttt gacgatttct tcttcaggtt gtaattcttt tggtgcaacg atacgcatat
    10141 tcgctcctaa aattgcacct gtgactaata aagagttagc catattgttt cgaccatctc
    10201 cgcagtaagc aacagtgata ccttctactg ttccaaagtt ttcttggata gttaaaaagt
    10261 cagcgatcat ttgtgttgga tgccattcat cagtcaagcc attccaaact ggaacaccag
    10321 agaattctgc tagttcctct accatttttt gactgaatcc acggaattcg attccgtcaa
    10381 acatacgtcc tagtacttta gctgtatctt ctgtggattc ttttttacct aattggatat
    10441 catttgcacc tagatattca ggatgtgcac ctagatcgat cgctgcagta gtaaatgcag
    10501 aacgagtacg tgtagatgtt ttttcaaata aaagcgcaat attttttcct tctagatagt
    10561 gatgtgggat accacgtttt ttcaagtctt tcaagtgttc tgaaaaatcg atcaaatatt
    10621 gtaattcttc ttttgtaaag tctttttctg ctaataagct tcttccttgg aatactgatt
    10681 ctttcatatt aaacattctc ctttagattg tttttattta agattaaaga ggaaatcccg
    10741 acggcagctg acagtaagac tgccgtcagg aaacaaatta cttcaagtct tcacggacaa
    10801 gtggttggct catgcagcgt ggaccaccac gacctcgtga caattcactt gaattgattt
    10861 ctaacacttt gatgccatag ctacgaagca attcgttcga tacataattt cggttataag
    10921 tgactacaac acctggagcg attgccaatg tgtttgatcc atcattccat tgttctcgag
    10981 gtgcaacgat ctcatctcca ttaccagttg ggatcagtac aagatcatct aatccaagag
    11041 cagctttcat cgtttcgtgt aagttatctg aatgagtaat cttgatatca tcaccatctg
    11101 gttcgattgt aaagacatcc atcttaccgt ctttactttg aattgctgga tggatagtga
    11161 atttatcgtg atctaccatt gtaaatacgg tatccaaatg catcattgca cggttgttcg
    11221 gaattttgat tgccaacact ttttcaaatc ctgaattttt tgcgaatagg tggcgtgcta
    11281 atttttctaa agctttcgca ttagttcgtt gagaaatccc aacagctact actttatcgc
    11341 ttaagatcaa ttcgtctccg ccttcgatat gatctggatt ttcacgatcc aaccatactt
    11401 cgattccttt atttgcaaat cttggatgat gtttcaaaat atattctgta aacatcgatt
    11461 cacgacgacg tgcttcaaaa gtcatcttat tgactgtcat cccatttccc attgatgcgg
    11521 aagggtcgcg tgtaaagtac aagtttggca ttgggtccat gtaaaatgga tagtcatcgt
    11581 ctgctgacag atcatataga ctgctagaac gtacttcgat atcttttgta cgaaccccag
    11641 ccatgatttt atctaccatt tcttgcgttt ccattgatag aaggaactca tgaagaccct
    11701 ctcttacagc attcgatgca atatgagatt catccaacat tttattcaag aattgttctt
    11761 tgacattgcc tgcgtcaatc gcttctgccg ctaatttttc caagtaaaga gtctcgacgc
    11821 cttcattttg caatgtcttc gcaaagttgt catgttcttc ttgagcaatc ggaagatatg
    11881 gaatatcatc gaacaacaga cgatccataa tatctggtgt caaattttcc acttcttgtc
    11941 caggccgttt cagtaaaact gtctttaatt tccctatttc agagaaaacg tgaataggtt
    12001 tatccatgtt gtgttgcctc cttctttttg attacatgta taggatatcc tccttttgta
    12061 atcactttca taattttgtc tcattcaaaa tgagacattc ctcacattaa tattcgataa
    12121 ttaaataatt aaacgttttt ctatttttaa gcgtaattta ttcatttttt attctgtata
    12181 cactttcaaa aaaatattta ttcattgaaa aaattttttt gaataaatta aaaaaataaa
    12241 tt
//
LOCUS       DAYYJC010000078        12115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000078 DAYYJC010000000
VERSION     DAYYJC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.080"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..2056
                     /locus_tag="ACHA9N_002091"
     CDS             38..2056
                     /locus_tag="ACHA9N_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9085537.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    2126..2332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2483..4897
                     /gene="leuS"
                     /locus_tag="ACHA9N_002092"
     CDS             2483..4897
                     /gene="leuS"
                     /locus_tag="ACHA9N_002092"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9085538.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5245..6798
                     /locus_tag="ACHA9N_002093"
     CDS             5245..6798
                     /locus_tag="ACHA9N_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9085539.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7179..8963)
                     /locus_tag="ACHA9N_002094"
     CDS             complement(7179..8963)
                     /locus_tag="ACHA9N_002094"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085540.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8978..10699)
                     /locus_tag="ACHA9N_002095"
     CDS             complement(8978..10699)
                     /locus_tag="ACHA9N_002095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085541.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10799..11560
                     /locus_tag="ACHA9N_002096"
     CDS             10799..11560
                     /locus_tag="ACHA9N_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9085542.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3976 a         2186 c         2443 g         3510 t
ORIGIN      
        1 tattaataat agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga
       61 acggagacgt ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt
      121 gggaactgcg atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca
      181 agcggatgaa acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga
      241 aattgctact tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat
      301 cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta
      361 taatcttttt gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga
      421 atttttaaat aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc
      481 tgaatctttc aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta
      541 ctatgccggt acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac
      601 agggcgttat gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta
      661 cggattgacc caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg
      721 tggcaataat actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa
      781 ctcaggttca gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat
      841 ctcaaataaa ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa
      901 tttcatttat ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac
      961 gaataattcc ggtaacaata ccaattcttc aggaaatgct ggtacatcaa acggaggaca
     1021 aacaactggt gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga
     1081 tcacggaatt acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta
     1141 tccaggacaa cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg
     1201 ctcaaatact gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc
     1261 ttcaggaacg aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata
     1321 cggaattacg atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc
     1381 aggacaacaa ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg
     1441 ttcgtcaacc aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg
     1501 cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac
     1561 gatggatcag ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca
     1621 attgatcgtt aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc
     1681 gaaaccaaat acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat
     1741 gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat
     1801 ggatcagtta atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt
     1861 gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac
     1921 ctatactgtg aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat
     1981 ggctcaacta atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt
     2041 gattgtagct aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt
     2101 tgttactata aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga
     2161 agtttcagag agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga
     2221 gttgcccttg ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag
     2281 gccttttcac taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca
     2341 atctttttat aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa
     2401 ataaaaataa acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt
     2461 caatagaaca ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca
     2521 aaaatactgg gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt
     2581 ttacgcactg gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga
     2641 aggttatacg gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct
     2701 gcatccaatg gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg
     2761 aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc
     2821 tttaggattt agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa
     2881 atggacacaa tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt
     2941 agcggtaaac tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg
     3001 gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa
     3061 aatcactgct tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag
     3121 cattaaagaa atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa
     3181 agtggctggt acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg
     3241 tgcaacttac tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga
     3301 acaaaaagct gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag
     3361 aacggatctt gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt
     3421 taatggcaag gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg
     3481 agccatcatg gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg
     3541 actagaaatt cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga
     3601 tggtacgcac atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa
     3661 aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg
     3721 tgactggcta ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga
     3781 ggacggaaca gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa
     3841 aaacatcaag ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa
     3901 tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg
     3961 ggcaggaagc tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc
     4021 agattatgaa aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca
     4081 tgcggtactt catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt
     4141 tgttcctaca aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa
     4201 caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac
     4261 ttacggtgca gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat
     4321 tgcttggagc gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt
     4381 gatcgttgat gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct
     4441 tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt
     4501 caatacagca atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct
     4561 gccttatgaa tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg
     4621 tgaagaacta tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac
     4681 atacgatgaa gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa
     4741 agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc
     4801 attagccaat gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc
     4861 ggtaccaaac aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact
     4921 gatgagttgt gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt
     4981 gttatttcaa attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca
     5041 cgttcaatgt ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt
     5101 tgtctattaa tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca
     5161 aattcataaa ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat
     5221 gaagtatgag aaaaggagag acaaatggat caagaactat ttattatcga agtattatca
     5281 gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt
     5341 ttaagcttag tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc
     5401 catatgagtg cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac
     5461 ttttcgtggg gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt
     5521 tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa
     5581 cagtttttac ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact
     5641 tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt
     5701 gatgatttac ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat
     5761 atttttttac tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac
     5821 ctgacaacta agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca
     5881 atctattatg ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac
     5941 atgacaatga actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct
     6001 gatcctaagc aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca
     6061 gagacgcact ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt
     6121 gattcgcaat ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt
     6181 tattggatgg acaacacata tgatatttcc gccgatttct catggtttat ctattcttct
     6241 ggggatgatg tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt
     6301 atcacccaat cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt
     6361 ctatttatct gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact
     6421 atggatggcg gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa
     6481 aatgtcagct tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat
     6541 acaaacaacg gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag
     6601 acagggggga tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg
     6661 ttagagacaa aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc
     6721 tgcctcttcc gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa
     6781 gtagaacaag gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa
     6841 gaaacaaatt caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag
     6901 ttgacaagag caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg
     6961 tcttattgcc gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa
     7021 gtcttttgtc actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta
     7081 ttattctttg tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac
     7141 taatcataca tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta
     7201 ttataaagac tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg
     7261 acgatatcgc cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg
     7321 tgtgcaatca caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat
     7381 gcttctgttc gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc
     7441 gctagaatga ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt
     7501 tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt
     7561 gcgatttcag ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata
     7621 ttatcagcta ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga
     7681 cgtagttctc cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta
     7741 ttcagttcat aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga
     7801 ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt
     7861 ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct
     7921 aaaagtcttg catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc
     7981 ctatcgattg ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt
     8041 ggttcagaaa tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca
     8101 cctgatagaa caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg
     8161 aaacgaatgg ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat
     8221 tgctttttat ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg
     8281 accactagat taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac
     8341 tgattctgtt cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg
     8401 ccaacgatca gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga
     8461 ccagcagcag ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact
     8521 tgagaaagtt gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg
     8581 tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat
     8641 gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt
     8701 aagactgaac agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg
     8761 aaagaaagct gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt
     8821 cccatgatca agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat
     8881 ccgtaaaaat atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg
     8941 ttcaacgttt ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa
     9001 actcctgata agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt
     9061 ttccttcatc taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa
     9121 caatcacgag agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg
     9181 ttttatagtc taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca
     9241 acgctcgagc aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag
     9301 caacaaagct gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct
     9361 tgatcgcttt agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta
     9421 aaattgtccc gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct
     9481 gttcttgagt catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt
     9541 cactaagtct caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga
     9601 tcgctgttgt ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga
     9661 tttcagtata tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg
     9721 gttcagctac tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt
     9781 ctatccgtcg taatgaggca aacgatttag ggataatgac caataccata gctgcgatca
     9841 tcaagtaagc aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta
     9901 atgctccttc tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca
     9961 tgagcaaaaa agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat
    10021 tagcatagtc agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg
    10081 cacggatcat attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt
    10141 ttggctggat actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata
    10201 cataaaaaac aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca
    10261 tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa
    10321 tcatttgctg gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa
    10381 aacgactgat atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt
    10441 ggaatccaaa cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg
    10501 ttcctagaaa cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc
    10561 cacttgctat acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg
    10621 cgatttctaa acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga
    10681 atgtttttaa gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc
    10741 ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat
    10801 gaaaaaaatg tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat
    10861 ccgctattgg gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg
    10921 attgtttgat atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga
    10981 tataccgatc aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac
    11041 acttgatgaa acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa
    11101 agagataagg gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt
    11161 tcctgtagaa gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga
    11221 aatcaaaaaa tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag
    11281 ttcacagacg atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga
    11341 cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat
    11401 gaaccatgcg gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg
    11461 gaaaacagga gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag
    11521 aactgaatat tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa
    11581 ctcgaaaatg atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta
    11641 atgtggggga ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct
    11701 ttaaaaatgg aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa
    11761 agtatggaaa aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg
    11821 agaagaaaaa ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat
    11881 aggtgcataa aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa
    11941 taaggcaaaa ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact
    12001 gtgactttcc tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt
    12061 ataagtattt agagattttt cttctaacat tcttcaaaca agaaaaatca caaag
//
LOCUS       DAYYJC010000079        11985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000079 DAYYJC010000000
VERSION     DAYYJC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.081"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            457..726
                     /gene="rpsN"
                     /locus_tag="ACHA9N_002097"
     CDS             457..726
                     /gene="rpsN"
                     /locus_tag="ACHA9N_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9085543.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            741..920
                     /gene="rpmF"
                     /locus_tag="ACHA9N_002098"
     CDS             741..920
                     /gene="rpmF"
                     /locus_tag="ACHA9N_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9085544.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            952..1329
                     /locus_tag="ACHA9N_002099"
     CDS             952..1329
                     /locus_tag="ACHA9N_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085545.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1350..1499
                     /gene="rpmG"
                     /locus_tag="ACHA9N_002100"
     CDS             1350..1499
                     /gene="rpmG"
                     /locus_tag="ACHA9N_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9085546.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1523..2506
                     /locus_tag="ACHA9N_002101"
     CDS             1523..2506
                     /locus_tag="ACHA9N_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9085547.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2536..2658
                     /locus_tag="ACHA9N_002102"
     CDS             2536..2658
                     /locus_tag="ACHA9N_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9085548.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2676..4199
                     /locus_tag="ACHA9N_002103"
     CDS             2676..4199
                     /locus_tag="ACHA9N_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9085549.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4223..4462
                     /locus_tag="ACHA9N_002104"
     CDS             4223..4462
                     /locus_tag="ACHA9N_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085550.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4655..5116)
                     /locus_tag="ACHA9N_002105"
     CDS             complement(4655..5116)
                     /locus_tag="ACHA9N_002105"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9085551.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5311..5529)
                     /locus_tag="ACHA9N_002106"
     CDS             complement(5311..5529)
                     /locus_tag="ACHA9N_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085552.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5647..7263
                     /locus_tag="ACHA9N_002107"
     CDS             5647..7263
                     /locus_tag="ACHA9N_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9085553.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7504..8844)
                     /locus_tag="ACHA9N_002108"
                     /pseudo
     CDS             complement(7504..8844)
                     /locus_tag="ACHA9N_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9282..9428)
                     /locus_tag="ACHA9N_002109"
     CDS             complement(9282..9428)
                     /locus_tag="ACHA9N_002109"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085554.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9467..9661)
                     /locus_tag="ACHA9N_002110"
     CDS             complement(9467..9661)
                     /locus_tag="ACHA9N_002110"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9085555.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9571..9903)
                     /locus_tag="ACHA9N_002111"
     CDS             complement(9571..9903)
                     /locus_tag="ACHA9N_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9085556.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10417..10608)
                     /locus_tag="ACHA9N_002112"
     CDS             complement(10417..10608)
                     /locus_tag="ACHA9N_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085557.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10702..11088)
                     /locus_tag="ACHA9N_002113"
     CDS             complement(10702..11088)
                     /locus_tag="ACHA9N_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9085558.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11782..>11985
                     /locus_tag="ACHA9N_002114"
     CDS             11782..>11985
                     /locus_tag="ACHA9N_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9085559.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNE"
BASE COUNT         4012 a         1853 c         2279 g         3841 t
ORIGIN      
        1 attttattaa acagcatcta attttgaata cacgaaagaa tcagtatgat tgaattagtt
       61 cacttaatgt ctattcttat aatataagga ataatcaaag ttttaaaaaa ttggcgataa
      121 accagttaaa cataaaagat tgaataaaaa gttatagttt ctttcatatt gccgttttaa
      181 gtagaggaaa agctacttaa aacggtattt gtgtttgatt ggactaataa aagtgaattc
      241 ttatttttca gagcctaaac tgcatattct tttaaatgat ttgattatct ctatgtaccg
      301 ataaattcgt tttcaaatag ttatctattc atatgagttt tatttcatgt tatttattgt
      361 tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat tacgatttga
      421 ggtgtctaaa tcaaaataaa aggagtataa aggtatatgg caaaaaagtc gaaaattgct
      481 aaagcaaaga aacaaatggc gatgattgaa aagtatgcag ataaacgtca ggaattaaaa
      541 gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc gaatcgtctg
      601 aggcttcgtg atcagacgga tggtcgtcca agagggtata tgcgaaagtt cggtatgtcc
      661 cgtattaagt ttagagaact tgcccatcaa gggttgattc ctggagtaaa aaaagcaagt
      721 tggtaaaagg aggaaatatc atggcagtac cagcaagaaa aacatctaag gcgaagaaac
      781 ggttaagaag aactcatcaa actgtagtaa aaccagaaat ttcatttgac gaagcgaatg
      841 gtgattatag acgtagccat catgtatctt taaaagggta ctataatgga aagaaagtaa
      901 ttaaaggagc ttcaaaatag tttaatatat aacttttagg agggaatcaa aatggaaatt
      961 atttacccac ctttagttga agaaggatta aagtattatc ttgaaacgac ccagcaatcg
     1021 ctagataaat gcacttttta tcgttcgatg gtagagagag gaattattac tgaaacgggt
     1081 ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag attactatga ggaccaaggc
     1141 ttatcatttg atgatttttt aagaatctat ccaatttttg aagagtatga cgaggagtta
     1201 tttcaatgta tcgatggtta ttgggagata cctgttgata tgaaagaaaa tttagtttca
     1261 caattggaat ctggcgagtt aacttttgaa gatgcacaac aaattcaagc atatcttgaa
     1321 gatcgataag tattgaagga ggaagattta tgcgtcaaaa tattattttg gaatgtgtcg
     1381 aaactggaga acgtctgtat ctcaccagca aaaataaacg gaataaccct gaacggttag
     1441 aattaaaaaa gtattcgcca aaattgcgca gacgggcaat ttttaaagaa gtgaaatagc
     1501 taagtggtaa aaaggtgaca aaatgggcat accagttaca atcgtttctg ggtttttagg
     1561 ggctggaaaa acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc ccagggaaga
     1621 aattattata attgaaaatg agtttggtga agttgggatt gatcacaaat tattagttca
     1681 tagcgaggaa cgggtgttac aactaaataa tggatgtatg tgttgcagtc ttcgcagtga
     1741 tttattagca atattgattt ctttactgga aacaatagaa gaacatcaac aaccaatttc
     1801 acaaatcatc attgaaacta caggtatagc agatccccaa ccaatcattc aaacactttt
     1861 aacagcgcct caactaagag gaagagtata tatcgatagt ttattgactg ttattgatag
     1921 tgacagtttt gaacttattg aaagagaacc acaagccctt aagcaacttg tcatggcaga
     1981 tcgcattttt gtgtctagaa aacaaaactt gaatttatct gcactaaaaa aattacagag
     2041 agaaatcaaa agtattaacc ctttatccga tattcgaatg ttcacacata gtgaaacgat
     2101 tcaaaaaaag gatttttata atttgaaaaa atttaatcat cccttagttt ttgaaagtga
     2161 ttcatcagaa aatcactata atggcaaata ctatcatcaa caccatcaag tacatgagtt
     2221 taaatcaatt ctcttaactt cagatcaaga cataaaaaaa gattttttac tccaatggat
     2281 tgactggctg attttaacca accaagggag cctgtatcgt tttaaaggat tgattgcttt
     2341 acaagagcag gattttatag ttgccatgca agaggtaaac gaacaggtta attttcaata
     2401 caccactcga caaaaagacc aaaccactat tattctaatc ggaaaaatgt tggatgaaga
     2461 aaggatcaaa cagagttttg ataaattact ggcggaaagc aaataatgat aagtagtgat
     2521 tgggggtaat ttctaatgga gaaaacggat atatctagtg catatcgacg acttaaaagc
     2581 ccgaatatta aaactagaaa aagggcatta aaaattatca aagaacataa acgaaataaa
     2641 atgaagaaat tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt gtaggactta
     2701 ttgggatagc agctctagga ttattgggag cttgctccag cactaatgat gcaaaagtat
     2761 cgaatgataa agatggaaaa ttagaaattg taacaacatt ctatccaatg tatgatttca
     2821 ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat ggttccagcg ggatcagaac
     2881 ctcatgacta tgagccctca gctaaggata tggcaaaagc tcatgatgct gatgtttttg
     2941 tatatcataa cgaaaacatg gaatcatggg taccaaaagc taaagaaagt tggaagaaag
     3001 ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt attgccaggt agtgaagaag
     3061 aagatcatga tcacggagaa gaagatcacc atcatgaact agatcctcat acgtgggttt
     3121 cacccaaaat ggcaattaag gaagtttcaa atatcaagga tcagttagtt aaattgtatc
     3181 cgaaaaaagc aaaagtattt gagacaaatg ctgaaaagta tttgactaaa ctgaaacggt
     3241 tggatgctga ctataccact agtcttaaag aagcgaaaca aaaaagtttt gtgacgcaac
     3301 acgcagcctt tggatattta gctttggatt atggattgaa acaagttcct attgctggac
     3361 taagtcctga agaagagcca tcttctggtc gattagctga attaaaagag tatgttaaaa
     3421 aaaataagat caattatatt tactttgaaa agaatgctaa tgataaaatt gcaaaaactt
     3481 tggccaacga agctgggata aaattggaag tactgaatcc tcttgaaagt cttacaaaag
     3541 aacaaatgga taatggtgaa gattatgttt cagtaatgga agacaatcta aaagcgttag
     3601 aaaaaacaac tatggttgct ggtgaagagg tagttccaga aaaagaagcg aaggatgaaa
     3661 agacagttgc taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa ttgagtgatt
     3721 atacaggcga atggcaatct gtttatccgt tgttaaaaga tggaattttg gatgaagttt
     3781 tcgattacaa agcaaaaatc aataaagaca tgacagctgc cgagtacaaa gattattata
     3841 ctactggcta taagacagat attgatacta tcaacattaa agacaacacc attgattttg
     3901 tagtcaacgg tgaacatcat caatacacat acaaatatgt tggatacaaa attttgaatt
     3961 atgaaaaagg aaaccgtggt gttcgtttta attttgaaac tgatgatgca ggtgctggtc
     4021 gttttaaata tattcaattt agtgatcatg gaattgcacc aagtaaggca gagcatttcc
     4081 atattttctt tggtggagaa agtcaagaaa aactctataa tgaattgcat aattggccta
     4141 ctttctatcc agcttcattg tctaaacatg aaattgctca agaaatgatg gctcattaaa
     4201 attcaatttt tggaggaaat acatggtaag aaaagtatta ttgtctatgg attccaagaa
     4261 tattgatgct tatcggcgtt atcgatttgc aaagcaaatg agattaaatg ggagtacatt
     4321 agctgatatt gctgcaactt tacatgttag tattgacaca gttcatagag tattaaaacg
     4381 ggatcctgat tgttgtttta tcaaggtcaa tactggaaga agtagacgtt tagcaaaacg
     4441 gcataatagc ttggccaatt aattgataat accagagtag atcatttttc ccttatttgg
     4501 atttgatctg ctttttatta tgtcttgaat tgtcaaatca agcgcaacac tttatcaaag
     4561 aagacttcat aaggtgttct ccaatctaaa cactttctag gtctgttatt cagtttttca
     4621 atcatctgat ttatcgttga gttatctacc gtagctatct ctttcgattt tggcaagtat
     4681 tctcgaatga gtctattcgt attttaattt gttcattgtt gccaagggca tgaggatctg
     4741 gaaaataaaa gggcatatta ttcaatgctt ctgtaactgc gaaatgattg gagaattctt
     4801 ttcctctatc tggagtgatc gttttgaccg cctcatttgg aatactgctt aaaagttcaa
     4861 tcattttctc gctcacataa gcggctttct ttctggggac cttctttcct gctaaaagga
     4921 agcatgattt tctgtctgtc aaagtcacta aacaagtacc accagttttg ccagctacaa
     4981 tatcggcttt ccaatgacca aaatcacttc tatcatctgc ggattttgga cggccatgga
     5041 tcgaatgagt aaggcaaatc tttccacgag actcgacatg attttttgta tgtcttgttt
     5101 ttcctcgatg gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg ggttctgtat
     5161 cgaaaagatc attgcaaatt gcacgttaga tcgtgataaa actaattgaa aagtccatat
     5221 gttcatgctt caaacgattc gagatttcct ctggcgacca ctgaaaatcg acaaataaat
     5281 gacgaacaat tgaccaaact cacggatcat ttagaatgtg ataacgtccg catttttttc
     5341 ttcgataagc ataatttttt tccgctttgg aaggtgaata gtttttttct tgtttcttct
     5401 cagttcatgt aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta tactaataga
     5461 aaatccttct tcgtgaagca gaaagattct ttctcgttcg agtatagtaa gatgacgata
     5521 ctggctcata attattttcc ttctaggagt gttctcgaca aacttatact agttggatat
     5581 cgattatgac tcatttttat gtgttgcact taaagtgtaa attcaaggta tagaaagagg
     5641 ttccctatga aaaaaatacc tattacaatc ttaactggct atcttggttc aggaaaaacg
     5701 atacttctaa atcatattct gtacggtgat cacggaaagg aaattgctgt cattgtaaat
     5761 gacatagggg aagtaaatgt tgatggggta ttagtcgaca agaaaggttt taaaagaact
     5821 ggagaaaaac ttgtttcgat gccgaatgga tgcatttgtt gtacattacg cgaagatttg
     5881 attgttgaac tagatcaatt agtgcaattt tctgacttga atcaaattgt tattgaagct
     5941 tcaggaatca gcaaaccaat tccaattatt caagcaatta tattagctaa aaacccttta
     6001 gacattgatt tgacccaacg attagagatt ggtgccatta ttatagtagt ggatgtaaaa
     6061 cgtttttggg ataatttcga aacgggtgaa aagattcagg aaagaaaagc tgaaggtaat
     6121 gtagttgaag aagaacagga tattcgtggc ttgttaattg atcaattcga gtgttgtaac
     6181 atattacttt taaataatag tgacttagtg tcaaccaaga cgatttacaa aattaacggt
     6241 ctaattgcta agttacagtc caatgccaaa ataattagga caattaaagg tgaagtagac
     6301 ttatcgaaaa ttctaaatac aaatcttttt aatatagatg agacttccta ttcagtgggc
     6361 tggttcaaag agttagagct tggctatcaa aatcatattc ctgaaacaga agaatatggt
     6421 atctcaagtt ttatctatcg aaatcggtta ctccttgacg cacaaaaatt tgcccatctt
     6481 acggataaaa attttcctgg caatattaca agagcaaaag ggtttctttg gtttgcccaa
     6541 catagtgatt tttcgacgat ctttgaaaca tcgggtagct ataaaagtat tgaatcatta
     6601 aattattggg tagctagttt acctaaaatt gagtgtcaaa tgattataca aaaaaatccc
     6661 gaattgaagg aagcctggca tccgttgttt ggtgaacgca ttaataaact agtttttatt
     6721 ggtatgaaga tggatcaaca atgtattaag gaacaacttg agggttgttt aactgattct
     6781 cttaatgcag ataatttccg aaaaatttca ccatttccat ggaaaaagtc taatatgttt
     6841 agccagatat cgttaatgaa atttttaaga aaagctggtg tcaagatagg cgatgtcata
     6901 attattggca ttttgattat tacctctttt attcccttat ttgtattctc tatagaaaag
     6961 gagaatgaaa caactgaaga tgctatcaaa tatgccatcg ttcgaattga tggcaaggaa
     7021 atggatcgct tcgatttgga taagatcaat cataaactcg tgaattatca tcctgcgaag
     7081 ggacaatata atattgttga gattaaagat ggaagaattc gagttaaaga agataatagt
     7141 cctgatcaaa tcgcggttag aactggatgg atctcaaaac ccggtcaaac gagtatttgt
     7201 ttaccccata agttagttat tagtattgag aaaaaagaaa gcacagatta ctacatttac
     7261 taatctggaa atagatgagg aggtttttaa taagaaaaag atattattat taataatagc
     7321 aggggcttta ttaagtgtgt taagcgcttg tggacaagaa aaagaagcgg tgacaacagg
     7381 cacaagtatc tttaacggga cctgcaatgg tttagttaac agtcggttag attacttatt
     7441 ttggctcttc gtcaatgagt gttggtgaag aaattcaata gaataatcga gatactcaca
     7501 cggctagaca gctatcttgt ctagccgtgt tgttgttctg gctttagtca gttgggtagt
     7561 attcttagtt ttcttaaaat attttcctcg atataaaaag attcgttctc ggtagttttg
     7621 aaagtttcta tagccatagg caattcgttt tagtactttg atatggttat tcaaacattc
     7681 tatagggccg ttggaatagg gaacgcgcaa cgctcgtttg atttctgttt ggtatttctt
     7741 gaaggtcgtg aacactggtt ggtagtgctc tggcaactcc ttgtagtctt cttttaggag
     7801 cgcgacgaat tcaggaacat cttgtcttct gataacggaa agaaaatcct ggtagacttc
     7861 gtagcctcgc tttaaggaat catcataaga taatagccga tcgactatct ctgcttctgt
     7921 cagatgatct cgaaatgacg gacgccagta gcgatgctga cctttcaatt cccacgcatt
     7981 cttttgaagt agtttccagt atttctttaa gtgcttcgac cgacgatccc cttttttaaa
     8041 ggtcttcatt acttgaattc ggtggttggt aaaagcccga ccaatgtgtt ggctaatatg
     8101 aaagcgatcg gcgacgattc gggcattcgg aaaacactct ttcaccaatg tgcgataggg
     8161 tgtatagaaa tctgaaacca ctagacgcac ttgttcacgg acttttatag ggtagcgcaa
     8221 gaaatagtcg cgtaaaggat tgagttgccg attttccaca atatcaatca gttgatgggt
     8281 cttgccgtcc atcatgacaa agctcatggc tcccgagaca tttttcactg atttgaattc
     8341 atcgaaacac accacttctg gtaacagctg gctcaaagaa atggttttag gccgaaggct
     8401 gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc atacgtgcga tcaaagacat
     8461 agaaagaggt tcgcttaata gctcaagaat ggcttgtttg actctgcgag caatggagca
     8521 gcgacgatca ctaactgaat cttctgctaa aaaagtcttt ccacagtgtt tacatttgaa
     8581 gcgctgtttg ttaatccgca gaatggtctt gtagttactg acatcattca ataagatgga
     8641 tacttttttc cagccctatt taatgatcgt ttgttcattt tgatagtggc gatgaaagca
     8701 ttcctccggt tgataggtca atgtgccatc aaaaacaaaa catgtttcgt cattgattcg
     8761 ctctttgcga aaggcatctt catgaaagat aagatttagg tctagaatat ctagtaactc
     8821 tttggtaaac tctgaatagg acatcttgaa acactccttt gaatggtttt cgtcgacttt
     8881 attctaaagg attttaaggt gtctgtttgc gtttattaga aaaaagaaaa agtattggca
     8941 acagaaggcg tcaccaacac tagatattat agatccctta ttttcttcgc ttcaaattgt
     9001 caaggagtca tcataaattt tgtatggatt cttttgtttg ggtagtagtc ttctaaataa
     9061 tcttggatta acaccttcat ctctatttta cttttatatt tgttggagtc gattaattct
     9121 catttgattg atcgatgaaa cgcttcaatt actgcattgt caacaggagt cccagtttgg
     9181 ctcactgaat gtgtgatgtg gtggtcgttc aacagctctt gataaataaa agaccgatgc
     9241 tggctacctc tatcggaatg aattaccgta ggcaattctc cttaataaat ttgtaatgga
     9301 cggacgacaa tctctttttt catgtgtgaa tctaccatat agtaacgtag aatacgagtt
     9361 gctaagtcaa tataggtgga caaatagagg cgaccgtcat tacatggaat gtaagtaata
     9421 tccatcaccc aaacagcatc aatttggttc tgttcaaatt atcgattcaa cattttttta
     9481 ccatttcttt gattgttttt tctctaccat gagaagattt gcgtcgatga aatcctcttg
     9541 tgcataaatt catttcacgc ataagaacag ctacaacacg tttattgatt tcgattcctt
     9601 catcgtaaag tttttgagtg atccctggag atccgtatcg atctttgtgt tcgtagaaca
     9661 ctcgtcttcc tcttttagtc gtttattttc ctgttcaaga atgtagaaat ctgctttatc
     9721 aggtaatctt tttccttgtc cagcaaaagc atttttcccg tatcgacgat attctgaaac
     9781 ccatcgggaa attgttggac gagagatatt gtattcttca gcaattttct tggtagttac
     9841 ttttttctca aggatatcct gtgaaatctt tagtttaaat ttacgatcat atgtttttct
     9901 caatagagtt cattccttcc ggtaacgtaa ctgatagtca agaaaagtgt atcatagccc
     9961 aacttagtat cagggtttgt caactaaact gtggaagttg atagataaga gtttttaaca
    10021 accaaaattc tcttgactat ttttgaaatc agacaaattt tgattggaca catagttgaa
    10081 taaatctacc gtcgttacgg aaggtaaatc gcatgctttt caattctaga ggagaagtcc
    10141 tcgttgaagt accatagccc agttctggat tcgacctcct gcccacttca tacgtaaata
    10201 aagtaatttt aggagtgaga ctgtgacaaa agttattttt aagctaaaaa cgaattaaaa
    10261 aacgttgata attaaaatca ttcgttttag attctatgaa tcattataga acgctgtaaa
    10321 atcgatactt tataattata aagaagatag tttcaaattt attgttcgaa atttgaaact
    10381 atctttttac ttatgtcaca gactccctta gttgagttat tctttaattt cgaatttcga
    10441 gatagctaga tatattgcaa gcatacattc ttgataacaa tcttgatcat attttccagt
    10501 tctccaggat tgcttatgaa tcaaagagct aaatcttttg agaattgcct caacagcttc
    10561 ttcatttcca gcctttgcta aaatgaaatc ttcttttaaa gaattcataa atctaccttc
    10621 ttcccatgga cttataatct atttctttgg gaaggaggag aacgagcaaa gaagcataca
    10681 aaattagtta ttatgcagat attaaaccag cattttttga atttttttgt aaagtcgatg
    10741 ttttaaattt gtcgcaccct gtcttgtaat ccccagaacc tttccaattt ctttatcagt
    10801 tttatcaaag ataaattttt cgataagaaa aatttgttca ggttctttca acaaaggaag
    10861 gatttttgct aattttatat cttcaacaag gagagtcaca tttaaaacat taatcggttg
    10921 tttaaatttt atagtatctt tagtaaacaa atatggtggg agttgatcaa gttgttcatg
    10981 tttttgaatt tcttcttgtt ttttataata agtattagcg gtgttcaaaa tgaccttttc
    11041 aaagaatcga atcaactggg catttacgcg ttcgtcgtta tctgacatat tgcacacctc
    11101 ctccattaag atttaaaggg aaaagaggca aaaaacacga aaagaagcta atgcaattat
    11161 gctgcgctgc gaataacttc tataagctca catgcattga taggactgct gatgtttatt
    11221 ttcaaaaatg tttttttagt tgataatgga tagttatctt tatacttaga aaagaaaatt
    11281 catatatctt cttaaatatc aaacatgtcc ttatggctaa gaaaatgtct tgagcttttt
    11341 acgtataata aatcttttct aaaacctcct tttggataat attcttattc gtgtatagat
    11401 tgtatttttt atttcttccc ccttcataat taacaactag aaaagagttc gaatagacac
    11461 gtatttgaac gaaaattttt attggattcc caaattggaa tatttagaaa cgaaaaattc
    11521 ctattatctg tatagtgctt agataataac ggatcataat aattattgtc tttgaactag
    11581 atatatgtaa atagtattca tcatgatgaa aaatgatgag ttctcggtga taaaatgtac
    11641 tttttacggt ggaatataca tgaaagcgat aagtggatat tttataatga agtaagttag
    11701 cttattttga cagaggaata gtattttaga taacgtataa aatactattt aatgatgtag
    11761 aaaggaaaga gagaaacaaa agtgttttca gataaagaag tcttattaaa gtctcttcat
    11821 gtttgtttaa agttacctat cgtggttttt aattccgaat tcagtattat aaaagagtat
    11881 cgctcagata gaactactta ttttttttat gattataaaa agttactaac tgataatatt
    11941 aaaaaaacta aagatttcca tttctttact ggtgatttta atgag
//
LOCUS       DAYYJC010000080        11665 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000080 DAYYJC010000000
VERSION     DAYYJC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.082"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..214)
                     /locus_tag="ACHA9N_002115"
     CDS             complement(35..214)
                     /locus_tag="ACHA9N_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085560.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(230..430)
                     /locus_tag="ACHA9N_002116"
     CDS             complement(230..430)
                     /locus_tag="ACHA9N_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085561.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(595..1302)
                     /locus_tag="ACHA9N_002117"
     CDS             complement(595..1302)
                     /locus_tag="ACHA9N_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085562.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1295..1732)
                     /locus_tag="ACHA9N_002118"
     CDS             complement(1295..1732)
                     /locus_tag="ACHA9N_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085563.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1747..1998)
                     /locus_tag="ACHA9N_002119"
     CDS             complement(1747..1998)
                     /locus_tag="ACHA9N_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085564.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1998..2204)
                     /locus_tag="ACHA9N_002120"
     CDS             complement(1998..2204)
                     /locus_tag="ACHA9N_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9085565.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2641..2901)
                     /locus_tag="ACHA9N_002121"
     CDS             complement(2641..2901)
                     /locus_tag="ACHA9N_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085566.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3075..3179
                     /locus_tag="ACHA9N_002122"
     CDS             3075..3179
                     /locus_tag="ACHA9N_002122"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFM9085567.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3320..3805)
                     /gene="ssb"
                     /locus_tag="ACHA9N_002123"
     CDS             complement(3320..3805)
                     /gene="ssb"
                     /locus_tag="ACHA9N_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9085568.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3808..4707)
                     /locus_tag="ACHA9N_002124"
     CDS             complement(3808..4707)
                     /locus_tag="ACHA9N_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9085569.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4720..5313)
                     /locus_tag="ACHA9N_002125"
     CDS             complement(4720..5313)
                     /locus_tag="ACHA9N_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9085570.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5389..5538)
                     /locus_tag="ACHA9N_002126"
     CDS             complement(5389..5538)
                     /locus_tag="ACHA9N_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085571.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5554..7758)
                     /locus_tag="ACHA9N_002127"
     CDS             complement(5554..7758)
                     /locus_tag="ACHA9N_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9085572.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7769..9229)
                     /locus_tag="ACHA9N_002128"
     CDS             complement(7769..9229)
                     /locus_tag="ACHA9N_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9085573.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9290..11662)
                     /locus_tag="ACHA9N_002129"
     CDS             complement(9290..11662)
                     /locus_tag="ACHA9N_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9085574.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1956 g         4385 t
ORIGIN      
        1 ctatcacttt tagtttttag gataaaaagt gacgtcactt tttgagtgct ggtacctcaa
       61 atcctgcttt ttcttgggca atcaataaat ctaacgcaat acacataatc ctggattcac
      121 ttttgccgta cttctgcttt agttgctgca attctttttg tctcttttca tccaacgtca
      181 cgggtaaccg tttatttcct attgcaattg gcattagtac tctctccttt catcgaaata
      241 accctattaa aaatatggct gcaatcaact tgaatattag actcacaagg gttaacacaa
      301 agcgccaaaa gatcaaacaa aataccagta ttcctaagaa tactacgaag tttaaatgta
      361 gagctgcacc atgacgcata agtatctcgc caacaaacag tacgaacaat agaggaatga
      421 ttttattcat atggatctcc ttctttctct taggttatcg ggctttgccc gcttatttcg
      481 caccacctaa gcggcggggg cggttggatc gattggtgcg aaataagaac cattccctta
      541 atttcaaggt tttttgttct tatttcacac atcctcactt ttcggtataa atggttattg
      601 accttttttc ctttcaaatc gttgatatgg cattcgttta cggtcaagtt cattttggat
      661 ttcttctgta tcgtcgtaag tgtaggtgtc taaaaaatca gttaaagtcg tgtaccagtc
      721 gtcgcctggg ttatctactt cttctacata ttccctcaaa aatgcttcgg gtacagtgag
      781 ttcataatcc attcccatta acacgataga ttccgtccca cacgtcaaaa atctagtaaa
      841 tacaaggtaa taatcgtcta ccgtcgcatc tttgctgttt agctcttcca tttcttcttc
      901 cattaattcc atgctctcaa aaattgaata atattctgat tctgagtaat agtgtttcct
      961 gcaaatcgtg tagctgacaa agttttcgac cagctcactc ctttctaggt aaacattcat
     1021 gatatatttt tctgtttcta gtcgtagaag ctccgctcct ttttttaatg tgtcaggacg
     1081 acgtttatac acgtctaaac gattatggct gactgtaacc ttcgtttgac tcgtttcact
     1141 ttccatatac aactgttctt ccactctttg aataaagtac ttcatttctt ccatacaact
     1201 aaagtctttt tgaatccgtc tgattacttc attcaagtgt tctttgtttg ttaaaaattg
     1261 cgaaacatct tctgttgtta gctgataaac caccttattc attatctttc tcctccgatt
     1321 caatcgtata aaatgcacca tttttttcga tattttcaaa agtatctagc cagtcgctag
     1381 ccattgcttt agctttctta atctgtgcga attttgcata agtcagttct aaggcttgat
     1441 attcggaagg tttgatagtg cttggtttga cacctacttc caagcatagt aaggcttgga
     1501 aaaaccattt tccattttgt tttttcagtt cttccagtgt tcgatagtac gtcgcaatga
     1561 gattcatttc gacacctgat tcaatcattt cttccactgc gactgacaat ttttccttgt
     1621 aatgtttttt gggtacacgt gtttcatggt ataggaacgt catatcctcc attttttgtg
     1681 ccattttatc caaaggtaat ttgacaaatt cgtcatagta attttgagcc atattttttc
     1741 tcctttttag ttctctaatt tggttaacca atattcgatt tcataagttt ccatgttttc
     1801 ttgataatgc agttcatatc ctgcaatctt cactaggtct tcaaatagca tagtcggtgc
     1861 atcctctact aaaggggttt ctatcgtcca gtaccgcgga ataagtaaag cttctgctac
     1921 tttttcagaa atgacttctt tgtccaccaa aatttgaacg acttctttta cagtcaataa
     1981 tttgtgttgt gttttcatca ccgtttcttt actttctcat caatcaaaac ataatcgcca
     2041 taagacaagg tatatttttt cccgcatgtt ggacattgat acaaacgata gcttttgata
     2101 catttcgctc tttctccaca atcgcattcg acagtatgtg gtgcatgata gtttgggaat
     2161 aattcgctga agggaacacc ccaataatca aatttctttc gcatgattga ttctgcctcc
     2221 tcttaggaaa ggttttattt cctttcgtta gttctttctt tattgatttg ttggccagaa
     2281 tgaatcaacg aaaagcgaca aggacaaggg cgcgcagcgt gccatttacc cttgtccttg
     2341 tcgctttttg gtgataaaag gcaacttgtt gccggtctgg caaatcaata aagagatagc
     2401 gaacaggaac gaaccttttg gtgagaggaa gttttttcta ttttgtcttg ctttgcaaga
     2461 caccatatct tagtgaacgt gcaacgttca cttcctttct tctcgaacgg cggtggcggt
     2521 tggagtgaga gaaaggtttt tcaattgaga aggcacttgt gtcttcctaa aaaggtcttt
     2581 tcctgtttct atgtaaaaca aaaagagctg caatcctcat ttaggactgc agctcttctt
     2641 ttatatgccc atcgatcgcg taaaatgatc ggggtttcta atttgcatcg attcaatgac
     2701 taattgattc cgtttattga aatgacctgt gacggcaata ttgtattttc catcatccat
     2761 aattaacaat ttatcgataa tttcaatatc tgcaactaca caattaatcg attcatgaat
     2821 tgtttgtaaa gtaaagcgaa ccaataatgg ttgtgttttt attactcgga ttttatcaag
     2881 caaaccaaca tatttgttca tcacgatcac ctcgctaaaa ttatagcaaa aacagctaaa
     2941 attgagaatt tttattcgat tttagatatg ctataatgat caagacagta aagtttgtga
     3001 cggtggctat ccgattgaaa gtgaggtggt gcgtatgtgc gtacttccaa aatcttacag
     3061 aaaggagaag ccttttgcct gttgaaacgg cgctaggatt aatgattagt ttcggttctt
     3121 tgatcgcaac aatcattttt ggaatcttag cagttgtaaa caacaatcaa aaaaaataac
     3181 cgtcagcttt agccggtcga cgattatttt tcaaaaataa tattaagctg gtcaccgtct
     3241 taaacggtgc tgttagggga gttgttagcg caactcctct tttcgtatcc attataacaa
     3301 ctttattgat cttttacaat cagaatggta aatcatcatc actaatatcg attgattgtc
     3361 cgccaaatgg attttgtgaa gcgttcaatc cattgttgtt aggcgtagct acatcccgtt
     3421 gttcaaaggt ggtagggctt ggactgctgg acgattgatc agcgtgcgca cggctttctg
     3481 tcgctgcttt actttctaat agttggaaat tatcggccac aacttctgtc acatacacac
     3541 gttgtccttg ttggttatcg taggttcggg tttgaatccg tccaacaacg ccgatcaaac
     3601 tgcccttttt agctaatgtc gccatcgttt ctgctggttt gcgccaaatc acacagttaa
     3661 taaaatcagc ttctcgattg ccgttttgat tcgtgaaatt tcggttgaca gctagggtaa
     3721 aagtcgctac tgcagtacca ctcgcggtat aacgtaaatc tggatctttg gttaagcgtc
     3781 ctactaaagt tacattattg atcatcttta tctctccatt tcttgattca tttgttgtga
     3841 tcttttaaac tgccgttttc tttcttgatt ttcaattttg atcacacttt tcattaggtg
     3901 atcaatttcg ttgactgtat ctgtaaatga aatggaattc gcaaacgtgt taactgagcg
     3961 atatttttcc cactgctttt ccaaatagtc atttcctctg atcttatcca tttctctttc
     4021 ataataaaag gacaacggtt ttgtatcccc acgttttgta aatgtatttc gaacactttt
     4081 gtataactct ttcgtatcga tattttctag tttcggaatg gtaaataagt cgtagtaatc
     4141 ttttgctctc gaattatctg tgccaaagct gaacgtagca cttagctttt ctgcaataat
     4201 ttgttctgtt ggataagctg gaatctccac ttttttatct tcaaacatta attggtgata
     4261 gtgtgtatct acttcaggat aaatagaatc tcctgttgtc acgtccattt taaacggtac
     4321 acgtactttc tctaaattag ccaccaattt aatttgaaat ccaggataaa aatcggcttc
     4381 tcttgtttcc ttcattcctt gaatagagaa aacaatgcct tcttttgtgg gttttctagt
     4441 taaatcatta aaaatgctct tcaatttttc ttcagttaat ttgctatttc taaatgtcgt
     4501 atcgatatca attgttgtcc ttctatcgat tccatatttt gatcctaata aaaagccgcc
     4561 tttcaaaatg aagtcatttt tgtactttga ttcagaaatt tgttcaatta gacgatccat
     4621 cataaaatgc cgttgcacta actgtgcatt taatcctgta ctctttgcta aatttcttgt
     4681 ttttgctgca aattgtgtcg gtgtcatctt tttcctccat caatataagc tttgcatgtg
     4741 gtatttcatt tctttttcta catgtagttt tgtcatgtaa tctcttaatt tacttggatc
     4801 tctcaatggg tcttccatgt actcttttaa tgcttctaat ttcatttcat attcaaattc
     4861 atatctcgga ttccaaatat cacaaagtgt acgctcttta tcatacatag taataggatt
     4921 tccttgaaag gaagttccct ctacttttcc tagttcataa acttctttct tcacgaaatg
     4981 aaattgtaac tgctgctctt ttgtaattga acgatcaacg ttatagccat ggggaaaagt
     5041 taagtgaatg aatttcggca tattcgtaga aagctggtaa atatctaaag tcgtttcatg
     5101 agagaagatt cccctggaaa atcgttgctg aagccaaaat aaatcatcta ttccatgacc
     5161 atcctctaca taataaccac gatcaacttt gatcaatttt ccttgattca ccaatctttg
     5221 tacagtggtt gctgcgattc caaatttcct ggcttccttc atagataaat ttccatcgcg
     5281 aatagccaac aattctttca gtttatcttg catttacttc ccctcccttc gttacaaata
     5341 cactacatca aatatatcat agtagtttat ttgtaacaag attaacgtct atcgatctaa
     5401 ttcgatttct ttttgaatgc gctgctggtt ctttaattgt tgcagtcttc ttttatttgc
     5461 acgtaatttc tcttgttcta aacgaatttt tgctttttga ttttcgattt cttgttgttt
     5521 gtgtgttgta tttcgcatag gataacccct tcattatttt gtagtgcggc gtgttctttt
     5581 cttatagttc gtttttttct tttcagaaaa ttcaaagcct gttgtaaagt cttcttttaa
     5641 aacgatcgtt gcttcaaacg aacctttctg accttttagt cctctgactg attggcttgt
     5701 tcggccgttt tgaatgatct ctttcagttg tgtggctgtg agtttctttt tggcaatcgt
     5761 tgtccataat ttaaaatcac aggcttcaga cgtacatgcc gccacatttt gataaagcaa
     5821 gacttcttgt tcccgacatt ttggacaaac acctaaatga cgcgttgctt cctgtttttc
     5881 actggctgct ttcacctctt gtaacgttga tccaaaatct gttgactgga tttgtcccgg
     5941 tacgacttcc aacaagtgag agacaaattt ttggatatta gctaagaaag tagcttgtgt
     6001 tcctttctgc tcaccaatct ttaataagta actttcccat ttggcggtca tttcggcact
     6061 tgttagcagc ggctcttttt ccacggcttg gcaaagtaaa atccctttat ttgtcaccac
     6121 aagcttattt ttttctacct tgatatattc cttttgtttc aaagtttcaa taatatttgc
     6181 tcgtgttgct tctgtaccga ttccttctac ttctttcaat atggattgtg cttcttcgct
     6241 atccacggtt tttccagctg ttttcatcgc tgtgatcaac gtaccttcgg tataaggttg
     6301 tggtggcttc gtttcttttt caaggacttg tagatctacc gtcaccgctt ctccttttgt
     6361 cactttagga agtagcggtt cttcttcctt ttcttttctg tcgctctctt tgccaaacag
     6421 ctttttccaa cctagatcta ctggtacttt tccgactgtc tttagctgaa gctgctgaac
     6481 ttgcgtcaag atcgttgttt cttcataggt atacttttcc gcaaacatgg ccaccgttgt
     6541 ttttaacact tgttcataaa tagcctgctg cagtggactt aattgttcaa aagccgctgc
     6601 cgtcatgacc gtctttgtag gaatgatcgc gtaatgttcc tggactttct tattattcac
     6661 atatcgacta ttcggtgtat gaataggagt aggaatggct tcggccttta aaaagtgttt
     6721 gtattcgtct aagtgatcca acagatacgc atactcccct tctgtaatat aaggggtatc
     6781 tgttcgtggg tagcttagat atttcccttc atacaaccct tgcatggctt ccaaagtatc
     6841 tttggcacta gctttcatga gctgattcat tttagattga aggctggata gagaaaaaag
     6901 acgtggactt gccgcacttt ttttctcggt ttcaacttgg tggatcgtac ctggctgttt
     6961 tcccaattga accccctttt cttttaaata atcttcaaat gcttcttgcg tcgcttgggt
     7021 ttcattcgga tcaagcttcg catcaaatgc gccattttga gcgattactt gggcttttcc
     7081 ttcaaaatat ggttcttttt ggaagtttct aatcttttcc tgtaattgat aaatcatgta
     7141 aagcgttggg gtttggactc tgcctaagga aaaacttcct ggtatgcctt tttgctgcaa
     7201 gagtaaacta tacagcgggc taccgttcat gccgatcaac caatcggcga tctggcgtgc
     7261 ttgtgcttct tcaaattttg ggtaggtctc ttctgcatct cgtaactgct ggaatccctg
     7321 ataaatggct tctttttcta aggagttgat ccaaagtctt ttaaatatct ttccatgatc
     7381 ggcgcctgct tgttgaatga tcgtccaagc tatcagttcc ccttcacgat cgctatctgt
     7441 cgcaataata atggtgtcgg ccgattgaag ttgctgtttc accaccttga attgcttttt
     7501 cttatcttta ggaacatgat agtgataatg tgtagggaaa ataggcaaat gctctaatga
     7561 ccattgtttc caattttcat cgtacatatc gggcgaatct aactcgacta aatgaccaaa
     7621 accatacgtg atcacagttt ctcctgaaaa caatcgatct tggatctcaa aatacccatc
     7681 ttttcgggta gctttagaaa agctatcggc ataagccttc gcttgtgacg gtttttctgc
     7741 taaaataacg gttttcatca tgacgatttc actccctttc ttgctgtcta ccttgttgaa
     7801 acaacatggc acgtttttgc tttctgatct cttgttggta gatttcatgc ttcaattgat
     7861 cattccaatc cttttttact tcaggtacaa attcatgttc atgattttta tagcctttgt
     7921 agtattgttc tgctttataa gagaaaagaa tctgagcatt aatatcccct ttttcttgat
     7981 aaaaacgatc aaaatcgtac gtaccatcca cgcgttcaca agcttttaat tcttttgcga
     8041 cttctctaca aatatctttc aaattaatag cttctattga atcgttaaca gaaagctgct
     8101 gaccaaaaga cgcatttact ttgtgagcgg aaaccaattg gttcgttaat tgcagattgt
     8161 tttctgtctt gtgtatggcc ataatagctt ctggtgtgat cttttcttct tttgcgactt
     8221 cttcataaat gactttgtat tcttttggca cttgccaatc attagcgatt cctcgttcac
     8281 aacgtacttt ctgattcgta aatggatcaa ctgcgcccat taattgttct ttttcgtaga
     8341 aaatatgtcc tgcacggtca ttatccacac aaatctcgat ggattgagga aaggcttgtt
     8401 tctttctcaa ttcagatact gcctcaccaa aataatgact gatcacatgg tgttttaacc
     8461 cttccatcga aactaaccac gtatcgttta actgatcacg atttagtgca gcatagctaa
     8521 gtaaatcaat actactctca aaaaatttta actgcttagg gtcaccgatc tttaggttaa
     8581 agccatgatt cgctgtcgaa tttttatcta tatgcttata tgtgcctctc tcaccaaaac
     8641 gttcataatc aatgaacgtt ccttgataac tcgcccctac tgtctcattt gtctgtggat
     8701 ctttccataa aaaggcgacc gcatgatcta aacgatcgtc tcgaactaag aaattttgcc
     8761 gttcatgttt tggctttagt tcggcaatca acccttcttt ttctgcataa tcaatcagcc
     8821 actctggtag ttttcgttct tccttcagat aattccttcc tttttctgtg agtggttgat
     8881 aatcaacggg gtgatctttg tacttttctg tctcaaaatg aagattttcc actcgttctg
     8941 ttttgtcttc attctttaga ataaaatcag tggctgcttt aaattgttgc ttagggtctt
     9001 ggatctcttt gtctatgaaa aaaagtttag ctaattcaat aatgtcgcca ctaatatttt
     9061 gactgttcca ataaaaacgt tgttttcttc gatcaaaaac aaatgaatca tggtcttcta
     9121 agcgatagtc tcgtccttta ttgactacag ccattccatt gtttcgagca aaatccacta
     9181 tatcgatctg tttacatcga tcaataagac tcattttttt ctcggtcatt ttaaaacctc
     9241 ccaaaagaaa aaagactatc cgtttttcag atagtctttt ttacttgttt tatacacgtt
     9301 ctttttgtga caattgttgt tcataataga tttcattttt tcgttttgcc acttctgaca
     9361 ctgtctcaag tgaaaatcgg aatgaatttc ctagcgcaaa ttcttctgaa ggtaacgccg
     9421 tttggatctc ttttccataa ctttttcctt ttaaaattac ttgattatgc tgtggatctt
     9481 caataaacgc cgcatccttc aaatgtaacg ttcgacccgt atcttcttcc attactttcc
     9541 cgtctttctt gtcgatccaa agtgaaccaa caggaacaat attcttttgt tctgccgttt
     9601 ctacagtatt tttatgctcc tgcttcactt ctgctggttg gatctctggt tctctttctc
     9661 tgtattgatt gatttcatta gaaatggttt gaatgaattc atttgcgaca cggctgacat
     9721 cattcatgat tttcccacgt tccgcagctt ccttatcatc caacgtctga ccattcttcg
     9781 tccatgctgc aatataagga atagctttct ccgaagtgtc cattccaaaa tgtttgcaca
     9841 caatataaga agtcatttct gcctgcaatt ctttagttgc ggtatctaat tctgaaaatt
     9901 tgtgcattct tgcatgggct aattcatgaa tacttgtagc taaatgctgc gtaggagtat
     9961 ttcgagtatt catgacgatt tcttcttcgc cagtaaattc gttacgaaca tatgcccccc
    10021 gtgcttgtcc taattctctt tgatagactt cactttctgt catatctcga atttgtattc
    10081 caatattgtt ggctaccgct tgaatcccag cttgaagttc tgactgtcct tttttatcca
    10141 attgaaaatc aaagacacgg ttcggaaaga ttttcggata gtcttcaggt tttaactgtg
    10201 tttgtgtgat atcaaacact ttttcaatcg tgtagtaagg ttttttgatc acttcgattt
    10261 gaccactttc gattcgtttt ttatcttctg gtgtagcttc tgtttctcga acaaagccac
    10321 gtcctttctc ataaaatcct ttgaccgttt ttctgacaaa gatcttgttc ccttgttccc
    10381 cttttttgat atagattcct tcttttttca acgcagcacg acctaaacaa gccgttgcac
    10441 ctggaaattg ttcctcaatc aataattgat ttctaaagga tcgttctgga aaacgagaca
    10501 taaaattcaa aagttctttc acgtcttcag gagaatgggt atgctttttc accgcatcca
    10561 gtgcctgttg cgaaagggat ttgacttctt gcttaatctc ttctgccgtt cgtttttgat
    10621 agcccttttg ttttgttttt gtttctttcg actgcatttg ttctttcgaa acaggttcat
    10681 ttttgaccgt ttgtttttgt tccttttcag gtacttgttc gcacaatttt gctaactcta
    10741 aatagatggg ttgattactg ttgacgccat cccctagatc cattcggatc tgatcgacta
    10801 cttctccgtt cttcacttct tcaaaataga atttataata acctgcttct gcggcgtatt
    10861 ggttatccaa cgttttgagt tgatcgatca attctaatgt tacgatctcc ccaccataac
    10921 ttttcaatcc tttttctgga tcattttcgt tgaattctac tcgatagaaa tgatcgtttt
    10981 ctttctcctt caaagaatca gttggcgcag cctcttcaag taattcacga acagctgctt
    11041 tttcctcttc cgacgtggct tggtattttt ttgattcctc aaatcccccg ttttcattca
    11101 gttctaaagc aacttttcga tagtctttca aaaaataaat ggttggattt tttttccatt
    11161 gcgtcactcg tccgctgtca ataattgctt gtacttcagg aagaagattt tcttttcctt
    11221 tttctgatct ttctttcagc tgttctaatg tttccaattt ccgttggtat tttcggatcg
    11281 tagcggctga aaaagtcgat tcaccttttt caaagcgttc gatttcttct ttgattcttg
    11341 gaatattttc gattgtcata tccaggccgc cgtacttgtt atagcccatt tcctttagct
    11401 cttcttgtct ttcaagtttt tctacctgtt tttcttgttg cttgatctct tctctcagac
    11461 tatgcacttt gttttctaaa cgttcttgtt gcctaaacca agaatgccca tttctcttat
    11521 cgttcattgg ctgaccgttg gtctttctct ggtgttcaaa catcttctca atttgttcgt
    11581 ttaattcatt ttgcaatcgt actaattttc ttttggattt tcccaagcga ctattgtcta
    11641 gtggttcttt ttcttctgcc atgtc
//
LOCUS       DAYYJC010000081        11446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000081 DAYYJC010000000
VERSION     DAYYJC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.083"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1183
                     /locus_tag="ACHA9N_002130"
     CDS             185..1183
                     /locus_tag="ACHA9N_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9085575.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACHA9N_002131"
     CDS             1295..1804
                     /locus_tag="ACHA9N_002131"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9085576.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACHA9N_002132"
     CDS             complement(1834..2493)
                     /locus_tag="ACHA9N_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9085577.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACHA9N_002133"
     CDS             complement(2495..3259)
                     /locus_tag="ACHA9N_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9085578.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACHA9N_002134"
     CDS             complement(3267..3944)
                     /locus_tag="ACHA9N_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9085579.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACHA9N_002135"
     CDS             complement(3981..5366)
                     /locus_tag="ACHA9N_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9085580.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACHA9N_002136"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA9N_002136"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA9N_002137"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACHA9N_002137"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9085581.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACHA9N_002138"
     CDS             6305..7162
                     /locus_tag="ACHA9N_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085582.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACHA9N_002139"
     CDS             complement(7243..7578)
                     /locus_tag="ACHA9N_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085583.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACHA9N_002140"
     CDS             complement(7645..9015)
                     /locus_tag="ACHA9N_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9085584.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACHA9N_002141"
     CDS             complement(9093..9629)
                     /locus_tag="ACHA9N_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9085585.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ACHA9N_002142"
     CDS             9938..11419
                     /locus_tag="ACHA9N_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085586.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2302 c         1854 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattc
//
LOCUS       DAYYJC010000082        11427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000082 DAYYJC010000000
VERSION     DAYYJC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.084"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..574)
                     /locus_tag="ACHA9N_002143"
     CDS             complement(29..574)
                     /locus_tag="ACHA9N_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9085587.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACHA9N_002144"
     CDS             complement(586..1278)
                     /locus_tag="ACHA9N_002144"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9085588.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHA9N_002145"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACHA9N_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9085589.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACHA9N_002146"
     CDS             complement(1594..2157)
                     /locus_tag="ACHA9N_002146"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9085590.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACHA9N_002147"
     CDS             2385..3047
                     /locus_tag="ACHA9N_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9085591.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACHA9N_002148"
     CDS             3171..4367
                     /locus_tag="ACHA9N_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9085592.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACHA9N_002149"
     CDS             complement(4468..5844)
                     /locus_tag="ACHA9N_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9085593.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ACHA9N_002150"
     CDS             complement(5881..6747)
                     /locus_tag="ACHA9N_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9085594.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ACHA9N_002151"
     CDS             complement(6836..7861)
                     /locus_tag="ACHA9N_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9085595.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACHA9N_002152"
     CDS             complement(7879..8553)
                     /locus_tag="ACHA9N_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9085596.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACHA9N_002153"
     CDS             complement(8737..9630)
                     /locus_tag="ACHA9N_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9085597.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACHA9N_002154"
     CDS             complement(9704..11401)
                     /locus_tag="ACHA9N_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9085598.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3386 a         2514 c         1962 g         3565 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctggtttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccacca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttatt
//
LOCUS       DAYYJC010000083        11313 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000083 DAYYJC010000000
VERSION     DAYYJC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.085"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1251)
                     /locus_tag="ACHA9N_002155"
     CDS             complement(<1..1251)
                     /locus_tag="ACHA9N_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9085599.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
     gene            1579..2058
                     /locus_tag="ACHA9N_002156"
     CDS             1579..2058
                     /locus_tag="ACHA9N_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9085600.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            2091..4319
                     /locus_tag="ACHA9N_002157"
     CDS             2091..4319
                     /locus_tag="ACHA9N_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9085601.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            4448..6091
                     /locus_tag="ACHA9N_002158"
     CDS             4448..6091
                     /locus_tag="ACHA9N_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9085602.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(6164..6709)
                     /locus_tag="ACHA9N_002159"
     CDS             complement(6164..6709)
                     /locus_tag="ACHA9N_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9085603.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(6732..7217)
                     /locus_tag="ACHA9N_002160"
     CDS             complement(6732..7217)
                     /locus_tag="ACHA9N_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9085604.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(7207..8019)
                     /locus_tag="ACHA9N_002161"
     CDS             complement(7207..8019)
                     /locus_tag="ACHA9N_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9085605.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(8158..9690)
                     /locus_tag="ACHA9N_002162"
     CDS             complement(8158..9690)
                     /locus_tag="ACHA9N_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9085606.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(9799..10830)
                     /locus_tag="ACHA9N_002163"
     CDS             complement(9799..10830)
                     /locus_tag="ACHA9N_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085607.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3716 a         2099 c         2183 g         3315 t
ORIGIN      
        1 tttgaaattg taaaagttgc gaaagccgta cgcattgcgt tttaatactt tgatgtggtt
       61 gtttaagcat tccaatggtc cgtttgaata gctgtagtga agcgcatttt ggatctgtgg
      121 cagaaacttt ttaaacgttc gtaaagtggt tgtataggtt tctggcagac cgctaatgtc
      181 tctagttaaa caatcctcaa atagtgactg atctcttttc tttaccgcat aaagaatgtc
      241 ttggtataac tgatagcctt ggcgtaagtt gggacaagca gctaacagtc gatcggcaat
      301 ctcactttca gtcaaataat ctttaaaact ggcacgccaa acccgttttt catagtttaa
      361 ctttgcttct tctttttgta ggagtttcca atagcgcttc aattttttcc ctaaccctcg
      421 ttgagagtgg gaatttcctt taagtcgtac cgtggtttct ttcactcgat gattcaagaa
      481 agttctgcca atatgttgga cgatgtggaa acgatcgatg atcaactgag cattcgggaa
      541 aagctttttc accaacgaaa cataaggcgc atacatatca ataacaatca attgaacggc
      601 ttcacgagtc gctaatgaaa accgagaaaa atagcgttct aaataaggca agcgtcgatt
      661 ttcgacaata tccaataatg catggctttg tccgtccatc atgataaaac tcattgcact
      721 ggcaacactt cgaaccgatt tgaattcatc aaagcataag acctttggca aagtctgttt
      781 gaaaggattc aatgggcggt agaatcgctt catgacacga tagaccgaag aagtggaaat
      841 atttttctga cgagcgattt ccgtcatgga agtattctct tttaagcgag caatgacaga
      901 ccaacgaact ttttgactaa tgaaacagtg tttttcagca acagaggtat ccgctacgaa
      961 tgttctttgg caagacttac atttaaatcg ttgtttcttc agctccagat aagtgcgata
     1021 ctcagacaca tcattcagta acaaccgaac ggttgtcgtc ccccaacgaa tgatcctaga
     1081 ggggacgaca caaagacaat gggaacactc ttctggcttg tacagaagag ttcctttgta
     1141 aatcaagcaa atttgccctt tgtatttttc tttagttaaa caattttcct cgaaatagat
     1201 atttaggtct agaatattta gggtatcttt gataagctga gtatgagaca tcttgaatcc
     1261 tcctatgaat tggtagccaa attaattcta aaggatttca gggtgtcttt tctatatttt
     1321 tttatacaaa aaagatgttg gtgaagttca ccaacatcaa aaagtataga gccaaaatat
     1381 tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga acgagatggt
     1441 gtttttattt ctatcaatga agctcttttt ctttgcaatc ctcacttcaa tcctctataa
     1501 tgtcgcatat agtttagtaa atgagtaaac tactaattta cttatttact aaaaataaaa
     1561 ggatgaggag ttaaaataat ggaaatacca acaaatttaa aacataaacc agtaatcgtt
     1621 gtagaagact atgataaagt tgatggaaga aatgcattaa atatggatgc aaaagggctt
     1681 tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg
     1741 cgacatactg gagaaaaatg gtcacgacag tcggaagaat taccaatcac acgtatttta
     1801 gatttagcga tcttagctgc gcaaggaagt ttatacttcc aagatgctta tcgttatgaa
     1861 aaattttatg atccagaaaa tccagtcgta gacatcattg gattacaagg gaaccgaatg
     1921 acggtagaag tagataccaa aaatccaaag atcgatgaag acatcatttt gtattacgat
     1981 acactacaaa aagacggaga actgattggg gaacgattcc gtattttaaa acgtctttta
     2041 gatgagctag gatattaata gacagagatg atcaaaaggg gaagagaagc atggagaatt
     2101 tctcactaac agaaaaccga accttttcac ggctagaaaa gcaaatgatc tggaagaaac
     2161 cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt
     2221 ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga aggagatgcc gggtcgggga
     2281 agacacaatt agccaaagca ctatctgctg atctgcaact tccctataca aaagtgacct
     2341 gttttgcaga tatggacaaa tcagatgttt tcggagcctt attacctgta gtagattcta
     2401 ataaggaaca agaccaagaa ttactagaag ctatttacca aacagataca cttgaagctg
     2461 tactgacact gatccagaac cattacggca ttgatccttt ttctgctaaa gaaaaattag
     2521 cagagctagt tcaaaggatc gaagctgatt atccagatgc aatccattac agctattacc
     2581 cttctgaaat cgttcgagcg attgaaaaag gctatttatt agagattcaa gagccgactg
     2641 tcatccgaga tgcctctgtt cttgtggcac ttaactcagc gctcgaacca aatgggatgt
     2701 tgaatttacc gacaggaatc attcgtcgcc atccagattg cgtcattgtg atcactacaa
     2761 atcgaaatta ccaaggaaat cgcccattaa atgaatcttt aagagatcgg atgcaacatg
     2821 cggaaaaaat ggatcttcct gccttagaag tcatggcaga acgtgcgatg gcgaaaacta
     2881 tgatccaaaa acaagaactt ctcttaaaga tggcagagat tatccgtttg ttggatgaaa
     2941 ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg ttcttatttt tattgggcaa
     3001 atacctataa gcaaggacaa gatttactcc agtcgattta tccaaaagtt ttgtataaat
     3061 tatccactga ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac agtggattgc
     3121 tactggaact tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata
     3181 caaaagaaag aaccattagc gccgaagaag caaatgaacg aaacatcact gaaaatgctg
     3241 aagaaattct gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca aatgagggag
     3301 aagagaaaca agaagaaaca atagaaaaaa acgagattcc tcctcaaatg caaaaagaac
     3361 gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca aacaggagat gattctcaag
     3421 aaatgtctgc ttctgacatg gaagcacatg ataaggaaat caagaagcaa ctgaataaag
     3481 aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga aggtctcatc gttcaccgac
     3541 cagctttcca aggaaaaacg ccggaagcac gtgagctaca catgcaagta atgccaatag
     3601 tagaatcact tagccggcaa attttagagt tactggataa tgaacaaaca gagacgtacc
     3661 aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa agtagcatat ggtgatttga
     3721 gaaattttga caaaaagaac ccgccacatg agcaaccttc tttagcggta gctgtacgga
     3781 tcgatgaatc tgggtcaatg atacgggatg atcggatcga agcggcaaaa aaagcagcga
     3841 ttgccattgc cgaattttct aagaaagtcg atatcccatt gttgatttac ggagatacag
     3901 cagaccgttc gtcaagagaa aagacttctt tgttttctta taaagaattt gaagacggat
     3961 ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg
     4021 ctgcacttcg tttagcagca gaaaaattaa acagacaatc cgcaacaacc aaactgttgc
     4081 ttaatattag tgacggtcag ccaaaagcat tgccggatta tacaggcaaa aaagcaaaag
     4141 aagatatcca gtctgttttg aaagaatatg aacgtcaagg aatcctattt gtctccgcag
     4201 ctattggcca agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata
     4261 ttactgactt gaatgaattt cccaagcaaa tgattcaact gattgcccgc tatctatgat
     4321 aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
     4381 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
     4441 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
     4501 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
     4561 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
     4621 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
     4681 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
     4741 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
     4801 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
     4861 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
     4921 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
     4981 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
     5041 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
     5101 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
     5161 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
     5221 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
     5281 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacatgcatt
     5341 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     5401 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     5461 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     5521 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     5581 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     5641 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     5701 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     5761 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     5821 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     5881 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     5941 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     6001 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     6061 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     6121 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     6181 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     6241 tcaaacgagg tctgacaggt acttggacat gcttgatatg cataccgatt gatgtgtcac
     6301 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     6361 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     6421 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     6481 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     6541 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     6601 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     6661 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     6721 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     6781 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     6841 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     6901 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     6961 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     7021 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     7081 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     7141 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     7201 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     7261 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     7321 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     7381 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     7441 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     7501 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     7561 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     7621 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     7681 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     7741 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     7801 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     7861 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     7921 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     7981 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     8041 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     8101 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     8161 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     8221 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     8281 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     8341 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     8401 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     8461 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     8521 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     8581 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     8641 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     8701 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     8761 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     8821 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     8881 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     8941 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     9001 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     9061 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     9121 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     9181 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     9241 aggttgttcc attaaataaa gaataggaat ctgttttgat tttgttccat ttgaaatttc
     9301 tctcactcgc ttttttgtct catcatcaaa taaaatgaaa gggtcttcta aacgatcgat
     9361 aaatacttcc attgttgttg ttggtagatg ataagaagtc atgacaggca ctgcaccaag
     9421 gtaagacaca gaaacagcta gaaggtatgt gtcaaaagcg gaactcttat aaataataac
     9481 tttgtctccg gatttcactc caagatgagc caattgataa gcccgtttca acacactttc
     9541 gcagcatttt ttgtaagttg tttcaagtcc taattctggg aaagctgaaa gtggctcatc
     9601 aaagataatc ggggtctcgg aaaagttagc tgcagcttct tgaaaattcg taaataagtt
     9661 caaaggggaa tacgttatgg tctccatcat tcacactcca ttatcttttg ttaagtaata
     9721 tacaaattca agataatcgt aacataatcc tttatataat aacatagttt attgtgaaaa
     9781 aaagatcata catttgtttt atatggaaga aacccgaacg aatgccgcta attcgtgata
     9841 agaaaaatca accatctttt cccagatatc ttgaaaagaa tgggatgaga ggtatcgatt
     9901 accacataaa taatttaccg cgtcagcatc atctaaatat agttctgcat aataacgata
     9961 tgtttttctg catagttctg gcgatacgtt tttatatact gtaattttgc tcaaaatttg
    10021 gcgaatagtc cgaggagaaa tttgctgatc atgtgaggaa cgaaaaacaa aagaattttc
    10081 gtccgtattt tgtgctgtta acttaaaata gttctccaga aatggcatcg cttcttttgg
    10141 tattggtact ttattcagtc tgttttcttt tctattcaga ttaagaatct gcttggtcaa
    10201 agatacatct ttacgtttaa gttctactac ttcatggatc tttaatccgc aatacagaag
    10261 aatggcagta attgcaaggt tcctcggtct attcttttct tcaaagctag cttctctgct
    10321 tgtttgaaag ccttcaatcg ggataaacaa atgctcaaaa tctttgaaaa gttctgcatc
    10381 gatatatcgc ggagctgctt tggttgtttc cggtaaactt gtacccaaaa gagaaatctc
    10441 ttcttcaatc agtccttttt gatacaaaaa tcgtaaaaaa tgatttaatg aatattgctt
    10501 ccttgaaatc ccgtaatagc tattttcaaa caattgctcc gtttcttggt tcgctgtagt
    10561 ttggaacgaa cgagtatgtc gatagatttt agctaagaaa ttctgataat cttgtttttc
    10621 taaatggata atcagctgat catccacttc ttcagaaaat acttttctca aataatcagt
    10681 aaacagatga aaatcttttg cgtattcata gcgactgcct aatgaaaggg aagaacgatt
    10741 ttcttctaaa aactgttgaa agacggaagg gagcaagtgt gtacgttgct ttaacttttc
    10801 ttgcagttgt tttctagttt tgctgtccat tacaaattcc ctccaatagt gttcattttc
    10861 tatattttac cgcatttcat caaatgaaga tagacagttt gtcttgatct taggaagcaa
    10921 atttttaact aagtactagc taaaagatat gaagtaagaa gatacatttt tagttatttt
    10981 attagtgcag tcattctttt gatcttttaa ttgatttatc ctctttttag ctatgtggtt
    11041 acttcatgag caatgaaatg gatttggttc gttctaatgg caggaggctc tttcttttat
    11101 ttccgaaaaa ttagaaaaca ttttttcaga atcgattggc tctccattta ttcaataatg
    11161 gtttgtctaa cgatattgtt gggataattc ttaaagaagt attagaaata gttggtactt
    11221 cattacttta catgaagatt tattctatgg taggagcaat tatattgaga attaatcctt
    11281 ttttatataa gttgtttgct tattgatgga ttg
//
LOCUS       DAYYJC010000084        11270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000084 DAYYJC010000000
VERSION     DAYYJC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.086"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACHA9N_002164"
     tRNA            2..74
                     /locus_tag="ACHA9N_002164"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACHA9N_002165"
     tRNA            78..150
                     /locus_tag="ACHA9N_002165"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACHA9N_002166"
     tRNA            155..225
                     /locus_tag="ACHA9N_002166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACHA9N_002167"
     tRNA            238..311
                     /locus_tag="ACHA9N_002167"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACHA9N_002168"
     tRNA            323..394
                     /locus_tag="ACHA9N_002168"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACHA9N_002169"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACHA9N_002169"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9085608.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACHA9N_002170"
     CDS             1983..3455
                     /locus_tag="ACHA9N_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9085609.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACHA9N_002171"
     CDS             3538..4326
                     /locus_tag="ACHA9N_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085610.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACHA9N_002172"
     CDS             complement(4393..5061)
                     /locus_tag="ACHA9N_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9085611.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACHA9N_002173"
     CDS             5176..6888
                     /locus_tag="ACHA9N_002173"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085612.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACHA9N_002174"
     CDS             6891..8660
                     /locus_tag="ACHA9N_002174"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085613.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACHA9N_002175"
     CDS             complement(8865..9431)
                     /locus_tag="ACHA9N_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9085614.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACHA9N_002176"
     CDS             complement(9431..10387)
                     /locus_tag="ACHA9N_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9085615.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACHA9N_002177"
     CDS             complement(10387..11043)
                     /locus_tag="ACHA9N_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9085616.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3405 a         2017 c         2415 g         3433 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa
//
LOCUS       DAYYJC010000085        10980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000085 DAYYJC010000000
VERSION     DAYYJC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.087"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1721
                     /locus_tag="ACHA9N_002178"
     CDS             90..1721
                     /locus_tag="ACHA9N_002178"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9085617.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1724..2803
                     /gene="uxuA"
                     /locus_tag="ACHA9N_002179"
     CDS             1724..2803
                     /gene="uxuA"
                     /locus_tag="ACHA9N_002179"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9085618.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2877..4304
                     /gene="uxaC"
                     /locus_tag="ACHA9N_002180"
     CDS             2877..4304
                     /gene="uxaC"
                     /locus_tag="ACHA9N_002180"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9085619.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4388..5035
                     /locus_tag="ACHA9N_002181"
     CDS             4388..5035
                     /locus_tag="ACHA9N_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9085620.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5164..6540
                     /locus_tag="ACHA9N_002182"
     CDS             5164..6540
                     /locus_tag="ACHA9N_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9085621.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6551..7345
                     /locus_tag="ACHA9N_002183"
     CDS             6551..7345
                     /locus_tag="ACHA9N_002183"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9085622.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            7358..9124
                     /locus_tag="ACHA9N_002184"
     CDS             7358..9124
                     /locus_tag="ACHA9N_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9085623.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            9152..10960
                     /gene="uidA"
                     /locus_tag="ACHA9N_002185"
     CDS             9152..10960
                     /gene="uidA"
                     /locus_tag="ACHA9N_002185"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9085624.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT         3885 a         1422 c         2245 g         3428 t
ORIGIN      
        1 tttttagttt tttcttattg acagcgatta cattcgattt tatactacaa ttgtaacgga
       61 aactaacata tcagtatgga ggggtaaata tggttttgat taaagactgg caagattcta
      121 tcgaaggatt gaacaaaatg caagttcaac taccgtattt taatgtcgaa gcggtcaaac
      181 aagaaggtca gaaacatcca gtgtggcttc attttggtgg aggaaatctt tatcgaggat
      241 ttcatgcaga aattgctcaa tgcttagcag atcaaaatga attagaatcc ggagtagttg
      301 tgtgtgagac atttgatgaa gaagtagtag aaaaagctta tcatgactac aacaatgata
      361 ttttggagat tgttatgggt gaagatggtg aactcaaaaa aagactttta gcagtaacag
      421 ctgatagttt atattgcaat ccaaagaata taacggacta tcaaagagtg gttaaatatt
      481 ttgaagatcc tacattacag tttacaacgt ttactattac tgaaaaagga tacagtttaa
      541 aagattctaa aggacaattt ttttcggtag tggaagcaga tataaaatct ggacctacca
      601 atcctcaaca tacgatgagt attgtgacat ccttgttgta tagcaggttt aatgctggag
      661 agttaccgat tgcgatggtt tctactgata atttctcaca gaatggtcaa cgttttcaag
      721 atagcatttt tatgattgca gaagcttggc aaaaaaaagg atacgtttct gaaaaattta
      781 ttgcttattt aaaggataaa gaaaaagtga cttttccttg gagtatgatt gatagaatta
      841 cgccaaatcc ttcggaaagt gtcaaaatgg atttattgaa ggcgggtttc gaagacttag
      901 atataattca tactgcgaaa aataccaata tcgctgcttt taccaatact gaaaaagtcc
      961 attatctagt cattgaagat agttttccaa atggtcgacc taaactggaa gcggctggtg
     1021 tgattatgac agatagagaa acagttgata aagcagatat catgaaggtt acgacatgtt
     1081 taaatccgct ccatacggca ttagcaataa caggttgttt gttggggtat aaatcaattc
     1141 aggcagaaat gcaagatgat gatttggttg gcttaattaa agggataggt tatgtagaag
     1201 gcttaccagt agttgaaagt ccaggcgtta ttgatccaaa acaatttatt gatgaagtaa
     1261 tcaataaacg tttgcctaat ccctatattc cagatacacc gcaacgaatt gcaacagata
     1321 cttctcaaaa agtagcgatt cgctatggtg aaacaattaa aaagtatgtc gaaaaagacg
     1381 gaaatgcaga aaatctaaca tttattccta tcgctattgc tgcttggtta cgttatttat
     1441 tggcagtcga tgatcaagga aaaaattttg tgccaagtcc tgatccttta ttggtagagt
     1501 tgcaggaaaa attctcagta ttgaaattag gtgaacaaga tcctgcaaag attcattgtg
     1561 cggtacagga aatcttagaa aataaggaaa tatttggttt agatttatat caagttgaga
     1621 taggaaagaa aattgaaaaa atcttttgcg aaatgctagt aggtcctcaa gcagttcgta
     1681 aaacaatcaa taaatatata acagaagttg gaggaaagta actatggaaa tgacttttag
     1741 atggtatggg gcaaatgaag aaaaaatctc actacaagaa attcgtcaga tcccagcaat
     1801 gaaaggaatt gttggaacat taatggatat accagccgga gaggtctggc cgagagaacg
     1861 aattaaagct ttaaaagatg aagtcgaagc caatggcctt accttgaaag ttattgagag
     1921 tgttaatgtt catgatgata tcaaaattgg tttgccaaca cgagatcaat acatcgagaa
     1981 ttataaacag acgattcgca atttggcaga atttgggatt gaggttatct gttataattt
     2041 tatgccagtc tttgattggg taaaatcaga tttggattat catttagatg atgggtcttc
     2101 tacgttagcc tatatttcaa aaaatattcc tactgatcca gaggaaatta ttagaaaagt
     2161 agaaaattct actaataatt ttgaattgcc gggttgggaa cctcatcggt tatcacaaat
     2221 caagcaatta ttagaagcct ataaagaagt tgatgaagcg aaattgcgag agaactttgc
     2281 atattttctc aaagcaatta tcccgacatg tgaagaagtt gggattaaaa tggcagttca
     2341 tccagatgat ccaccatatt ctattttcgg attacctcgt attgttaaaa atcgtgacga
     2401 tttagaatgg ttgtgtaatg tagtggattc tccttctaat ggtattacgt tatgtactgg
     2461 aagtattgca gaacgaccag ataatgatgt ctatgaaatt ctatctgaat tttgtcaacg
     2521 ggatcggatt ccttttgcac atgttcgtaa tatcaaatat ttaagtgata atggactcga
     2581 tttttatgaa gcgccgcata aatcagaata tggttctcta gatatgtata agatattaaa
     2641 aacaatgtac gataatggat ttaatggcta tattcgtcca gatcatggtc ggatgatttg
     2701 gggagaaacc ggtagacctg gttatgggtt atatgaccga gctctaggtg cagtttattt
     2761 aaatggtatt tgggaagcgc ttgaaaaggg ggcgagtaaa taattatata atggagtcta
     2821 tttagaagaa aaattttctc ttctaaaaac taagggaagg aaaggagtgc ttaaatatgt
     2881 ttttagataa gaacttttta ttagagaatg aaatagctaa aagacttttt cataaatttg
     2941 cagaacacca accaatcatc gattatcatt gtcatttaga cccagcatgt ttgctagaaa
     3001 ataagcaata caagaatttg actcaggtat ggttaaatga taaaggagct ggagatcatt
     3061 ataaatggcg attgatgcga gctaatggga tagaagaaga gttgatcagt ggtgcaggta
     3121 atgattatga aaaattttta gcttttgtaa aaacagtgga gaaggcgcca ggtaatccta
     3181 tttttgaatg gtctcatttg gaattacaac gttactttga tattgatttg acaatttctg
     3241 agaagaatgc accagaaatt tggcgacgtg ccaacgaaaa aatccaaacg gattcctatc
     3301 gcccaaaaga attgctgaag atgatggatg tgaaagccat ttgtacaacg gatgatccta
     3361 cagatgactt gcgatatcat actgagcttt ctaaacagga taattctttc aaggtattac
     3421 ctactttccg tccagataag gtctggagta tcaaatcatc aggattttcg aactatattt
     3481 taaaacttag tcaagcagcg ggtataccaa tcaaaaactt taatgggttg aagcaggcat
     3541 tgagccaacg agtggattat tttcatcaaa ttggctgtcg attagcagat cacggtttga
     3601 atacttattt ttatgaaata gcagatgaaa aaatactcga caatctgtta ataaaagctt
     3661 tagcaggaaa aacggatttc catttatcag aaattgcaca atttgttaca ggtattcaat
     3721 tacatttgat gtctgagtat acaagatatg gttggacgat gcaaatgcat ttaaatgctt
     3781 taagaaataa ttcaagtata ttaaaggaac aaattggagt agatgtcggt ggtgattcga
     3841 tgggggatca agctagtttg acacaaaatg ttgtagcatt gttcgacact gctgaaaaga
     3901 gtgggtgctt accaaaaaca attttatatt cattaaatcc aaccgattgg cttccactag
     3961 caacagcaat gcaaagcttc caagggggta tgattcaaaa aatccaattg ggatgtgctt
     4021 ggtggttcaa tgatacagct gaaggaatgc gtcaacagtt gactacgatg gcgcaacaaa
     4081 gtttgttgtc gaattttaca ggtatgttga ctgattctcg aagttttcta agctatcctc
     4141 gccatgagta tttccgtcga gtattatgtc aattactaag cgagtgggtt aaagcaggtc
     4201 gaattccaga tgatgaaatg tatcttagtc agatagtgga gaatatttgt tatcgtaatg
     4261 cgtataatta ttttaatttt tttgaaatcg aaagtagaaa ataatacaaa aaataaaagc
     4321 ttaaatttat ttattgtaag ttagtacaac tagctagagt atgtatccat aaaagaagga
     4381 gatagatatg aaaaaagtag aaattttaag taagttagaa gcggctggag tcattgcggt
     4441 tgtacgtgga aaaaataaag atgaggccat taaagcaata aatgcaatta ctgcgggtgg
     4501 aattaaaggg attgaactaa cttttacagt tccgaatgct gaagaaataa tcaaagaaat
     4561 tgctaaaaaa tatgctgatc aaaaagatgt aataattgga gccggtacag ttttagatgc
     4621 gtttactgct cgtattgcaa ttatggctgg agcagaatat atcgtaagtc catcttttga
     4681 tcgacaaaca gcagagattt gtaatttata tcaaattcct tatttaccag gttgtatgac
     4741 aattactgag ataaaggagg cacttaccgc tggagtagaa atcattaaac tatttccagg
     4801 ttctgctttt ggtccatctg ttgtaaaaac gtatcaagct ccattaccat atgtaaatat
     4861 aatgcctact ggcggagtaa gtctagataa cttagaagag tggtttgaag caggcgttgt
     4921 tgcagttggt gtaggtggga atttgttggc tcctgttgaa aaaggtgatt ttgaagaagt
     4981 aactcgaata gctagtaaat atatagataa gttttatgag ataaagaatt attaggagaa
     5041 aaaataaata atactataaa atgtaaaaaa taaatactaa ctctttatac ttcagagtat
     5101 agtacgtaag ttggcagttc aacagaatga aaacgcttaa caataggaag gagcgcaaat
     5161 tatatggaaa gaaaatttgg tctaagagac aaaataggat atatgtttgg agattttggg
     5221 aatgatttca ctttcatttt tgctagttct tttttaatga ttttttatac aaaagtattg
     5281 gggataagcg gagctaccgt aggaacatta tttttagtag caagatttat tgatgcagtt
     5341 acagacataa caatggggcg tattgtggat ggatcaaaac catctaaaac tggcaaattt
     5401 aaaccgtgga ttttacggat gagtggtcca gtcgctttag caagtttttt aatgtatcaa
     5461 acatcaatgg catcagcttc tatgagtatt aaagttatat atatgtatgt aacatatata
     5521 ctatggggaa gtatttttta tacatctatt aatattcctt acggttcaat ggcatcagct
     5581 atcacttctg ttgcagaaga aaggacagcg ttatcaacat ttcgtagtat aggagctacc
     5641 ttagcaggta tgttcattgg cgtgttaaca ccaattttta tttatacaag agatagttct
     5701 ggagctcaaa ttgtacgtgg tggatcaact tttacaattg tggcaggagt attttcagtg
     5761 tgtgcagtta tttgttacgt aatatgttat aaattaactg tagaacgggt taaaagtgat
     5821 gaatcgcagt taaaagtttc attgttgaaa tctttaaaag gattagctaa aaatagagcg
     5881 ttgctttcta taatagctgc agctattttt ttgttgcttg ctcagctttt aacaatgagt
     5941 atgaacaatt atgtatttcc agattattat aataatgcac agggaatagt tttaatgaac
     6001 tttatcaatc ctattttgat gttggtactt gtttcacctg taagtttaat tttcagtaaa
     6061 aaatacggta aaaaagaatt ggcgtcagta gggatgttct tctctgctat tgtatattta
     6121 cttttatttt tgataaagcc tagtaatatg tatgtattct tagttcttac ttctgttgga
     6181 tatatggggt taggtatttt taatacagtc atttgggcga atattactga tgtaatcgat
     6241 gatcaagaag tgaaatctgg ccaaagagaa gatggaacta tttatgcagt atattctttt
     6301 gcccgaaaaa ttggacaagc tttagctggc ggtgtcggag gttggactct ttctataata
     6361 gggtatgatt cgttagcaaa agtacaaagt ggagaagttt tacaaaagct gtataactct
     6421 tcaacgttga ttccagcagt ttgtttcttt gttgtaggat tgattttatt tttcgtatat
     6481 ccattatcta aagaaaaagt attagaaaat tataatttgc taaatcaaaa acataactag
     6541 gagaaaaaac atgaacagtt ggatattttg tattgattca gatggttgtg tcatggatac
     6601 aatgacttat aaacacaatc tctttttcgg gccaattgct gcagagattt ttgatgttca
     6661 tcatagggaa aaattcctac aggattggaa taatattaat ttatattcag agactcgtgg
     6721 aataaacaga tttttaggtt tagttaaaac attagaaaaa aatgatgtac catcgattaa
     6781 tcgattaaag cactgggtat ctgtaacaaa agaattgtct aatccatctt tgaagaggga
     6841 aatagaatac tctcagtcag aggatttatt aaaagcttta aaatggtcgg aagaagtcaa
     6901 tcgacagatt agtcaagcta aaggattaga taaacccttt ttaagtgcta gagatactat
     6961 aaaagctctg aaaaaatatg ggaagataat tattgtaagc tcagctaata aggaagctat
     7021 tcaagaagaa tgggaaagac atgaattatt atccttagtt gatgaactgt gttgtcaaga
     7081 taaaggaaaa aaagaagaca ttattcgaag tgtaattgaa aatggttgtg atttggataa
     7141 gatattaatg attggtgatt ctcctggtga tctagaagct gcaaataaga ataaagtttt
     7201 cttttatcct attttggtta ataaagaaaa agaatcatgg gaaaacttaa gaactgatat
     7261 tttatttaaa tgtttatcgg gagagtatat agatattgaa caaaaatata tagatacttt
     7321 ttggaaaaat cttactaata agtagaggag aaagaaaatg actataaaag tagacttacg
     7381 aaaaaaacca tatcatttag atgaagatgc tatttcttgg attgaagaga cgattgatgc
     7441 tatgacttta gaagaaaaaa taggtcaatt atttgttaat atgggatcta gtcgtgataa
     7501 aaattactta agtagtatgg tcaatcagta ccatattgga gctgtacgat ataatccagg
     7561 aactgctgat gaggtatatg atcaaaacta tatcctacaa acagaaagca aaattccttt
     7621 attaattgct gctaatacag aagcaggtgg taacggagct tgtacagatg gtaccgaagt
     7681 tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa tatgcttatg aattagggag
     7741 agtttctgga attgaagcat cggctattgg atgtaactgg agttttgctc ctattgtaga
     7801 tattaataga aattggagaa atcctatcat ttctagtcga tcttttggac aaagccctga
     7861 taaagtttta gaacttagtt tagcttatat gaaaggtatt caagagagtg gaattttgcc
     7921 tgcagctaaa catttccctg gtgacggtat tgacgaacga gatcaacatc tatcttttag
     7981 tattaatagt ttaagtacag aagaatggga taattctttt ggaaaagtat actcaggctt
     8041 aatagaagct ggattacctt caattatggc aggtcatatc catatgccag cttattcaaa
     8101 acattttaat cttgatctga aagatgaaga tttacttcct gcaactttat caaaagaatt
     8161 aatcactgac ttacttagag gaaaactcgg gtttaatgga ttagtagtta ctgatgctag
     8221 tcatatgtta ggaatgactg gggcaatgaa acggtcagaa ttattgccca cttctattgc
     8281 ggctggatgt gatctattcc tattttttaa tgatccagat gaagacttta attatatgat
     8341 ggaaggttat tacaaaggaa ttatttcaga agagcgttta cgtgatgcat taagaagaat
     8401 tttaggctta aaggctaaac taggtttgca taaaaaagaa aagaataaaa ttttgttacc
     8461 gaaacaagaa gctatggaaa aaattggttt aattgaaaat aaatcaattt ttaaagaggt
     8521 agctgatcaa gcaattacac tagttaaaga taaacaagat atttggccta ttgtaccaga
     8581 aaaatataaa aggattttac tggtagatgt acatggggta aaaggtgggt ttggtgaagt
     8641 agttgctgga aatagacctc aggccattga tttattgaaa cagattttgt cagcaaaagg
     8701 atttgaagtt gaaaaataca tttcacctga agaagaattg aaaggattat cgcaagaaga
     8761 aaaaattaaa aatatagctg gaatatatgc tgctaagaga ccaatcaaag atttaactga
     8821 aaaatatgac ttgattatta atatagctaa tgttaaccca ggtacagttc aaagaattgt
     8881 ttggccagca agtaaaggta ctccggatat tccattttat attcatgaaa ttccaacaat
     8941 ttttgtatct gtacagtgtc cgtttcattt agctgatgtg cctcaagtaa aaacttatat
     9001 caatacttat gatggaaaac aagaaacttt agaaatatta gtggacaaac taatgggcct
     9061 ttctgaattt aagggaataa gtccaataga tgcttattgt gggttaaagg atacatttat
     9121 ttaatattta aattatatag gagtgattta catgttatat cccattatga cagagacgag
     9181 gcaagtaatt gatttaagcg gtctttggag atttaagtta gatacaaatg atgacttaca
     9241 agaagaactt gctttagaac caatggttga aaaagagagt tatccaattt cagttccttc
     9301 ttcttataat gatttatttg aaacagagaa cattagaaat catgttggat gggtgtggta
     9361 tgaaaaagaa gtgattcttc ctaaaaaatt attagaagaa agattagttc ttcgttttgg
     9421 atctgcaaca catgaagcaa aagtattttt gaatggaaaa ttgattaatc aacacaaagg
     9481 agggtttacg ccatttgaat cagaaattaa tcagtttata aaagatggaa gtaacagaat
     9541 tacagtggct gtaaacaatg ttattgacga tagcacttta cctgtaggga ttttgaaaga
     9601 aacgatacag gaagatggag acataaaaaa agaaaatttg gttaattttg actttttcaa
     9661 ttatgcaggc attaatagac ccgttaaatt gtatacaact ccgaaagact atataaagga
     9721 tgtcactatt aatacaaaaa taattgattc agatgcaaat gtagaattta atatcgatac
     9781 agaatggacc ggagaaattc taatagcaat ctatgacgaa gaagctcaac ttatagcaga
     9841 agctaagggg aaagaaggag tgattcaact taagaaccca acactttggg agcctctaaa
     9901 tgcttactta tatagtatga aagttagttt aattaacgaa aataaagtga ttgatacgta
     9961 cacagaagaa tttggcattc gtagtattca ggtttcgaat gggaagtttt tgattaataa
    10021 taaacctttt tactttaagg gatttgggaa gcatgaggac acgtatatta atggtcgtgg
    10081 tttgaatgaa gcagtaaata tcaaagattt taatttaatg aaatggatcg gtgctaattc
    10141 atttaggact tcacattatc cttattcaga agaaattatg cgcttagcag atcgagaagg
    10201 tattgtagtt attgatgaag tacctgcagt aggattacat ttgaatttca tggctacttt
    10261 attagatgac aacgtagaaa aacatgatac ttggaaagaa atacggacag aaaatgctca
    10321 caaaatggcg attaaggaat tgatagaacg cgacaagaac catccgagtg tcgtaatgtg
    10381 gtctattgca aatgaaccag attcggattc tgaaggcgct aaagaatatt ttgaaccttt
    10441 agttgaattg actaaagaac ttgaccctca gaagagacct gtaacaatcg ttacttactt
    10501 aaagtcaaca ccagatattt gtaaagtagg agatattgtc gatgttttat gtttaaatcg
    10561 ctattatggt tggtatgttt caggtggaga cctaaaacat gcaaaatatc tgttgaaaaa
    10621 agagctagat ggatggatgg aaagatgtcc cgatacgcca ataatgttta cagaatatgg
    10681 agcagataca gtagctggac ttcatgatac agtaccaaca atgtttacag aagagtatca
    10741 agtgggttat tacgaagcaa atcacgaagt aatagataaa tgtgacaatt ttgttggtga
    10801 acaagtgtgg aattttgcag attttgaaac aagtcaaggg attttaagag tgcaaggaaa
    10861 taagaaagga atctttacta gagatagaaa gcctaaaatg attgctcact atctaaagaa
    10921 acgatggtca tcaataccaa atttcagtta taaaaaataa tttacctttt ataaggaata
//
LOCUS       DAYYJC010000086        10922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000086 DAYYJC010000000
VERSION     DAYYJC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.088"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACHA9N_002186"
     CDS             complement(<1..281)
                     /locus_tag="ACHA9N_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085625.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            453..1541
                     /locus_tag="ACHA9N_002187"
     CDS             453..1541
                     /locus_tag="ACHA9N_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9085626.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="ACHA9N_002188"
     CDS             1669..3045
                     /locus_tag="ACHA9N_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9085627.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="ACHA9N_002189"
     CDS             3446..5026
                     /locus_tag="ACHA9N_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9085628.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="ACHA9N_002190"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="ACHA9N_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="ACHA9N_002191"
     CDS             complement(5652..5972)
                     /locus_tag="ACHA9N_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9085629.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="ACHA9N_002192"
     CDS             complement(6120..8354)
                     /locus_tag="ACHA9N_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9085630.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="ACHA9N_002193"
     CDS             8833..9099
                     /locus_tag="ACHA9N_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9085631.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA9N_002194"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="ACHA9N_002194"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9085632.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DAYYJC010000087        10607 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000087 DAYYJC010000000
VERSION     DAYYJC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.089"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACHA9N_002195"
     CDS             34..657
                     /locus_tag="ACHA9N_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085633.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACHA9N_002196"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACHA9N_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9085634.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACHA9N_002197"
     CDS             2153..3034
                     /locus_tag="ACHA9N_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9085635.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACHA9N_002198"
     CDS             3037..3807
                     /locus_tag="ACHA9N_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085636.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACHA9N_002199"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACHA9N_002199"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9085637.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACHA9N_002200"
     CDS             5118..6281
                     /locus_tag="ACHA9N_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9085638.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACHA9N_002201"
     CDS             6398..6808
                     /locus_tag="ACHA9N_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9085639.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACHA9N_002202"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACHA9N_002202"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9085640.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACHA9N_002203"
     CDS             9381..9665
                     /locus_tag="ACHA9N_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085641.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="ACHA9N_002204"
     CDS             9825..10061
                     /locus_tag="ACHA9N_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9085642.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="ACHA9N_002205"
     CDS             10058..10456
                     /locus_tag="ACHA9N_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9085643.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3708 a         1771 c         2238 g         2890 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaa
//
LOCUS       DAYYJC010000088        10254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000088 DAYYJC010000000
VERSION     DAYYJC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.090"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..259
                     /locus_tag="ACHA9N_002206"
     CDS             110..259
                     /locus_tag="ACHA9N_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9085644.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            270..1784
                     /gene="dltA"
                     /locus_tag="ACHA9N_002207"
     CDS             270..1784
                     /gene="dltA"
                     /locus_tag="ACHA9N_002207"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9085645.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1781..2986
                     /gene="dltB"
                     /locus_tag="ACHA9N_002208"
     CDS             1781..2986
                     /gene="dltB"
                     /locus_tag="ACHA9N_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9085646.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3031..3264
                     /gene="dltC"
                     /locus_tag="ACHA9N_002209"
     CDS             3031..3264
                     /gene="dltC"
                     /locus_tag="ACHA9N_002209"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9085647.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3267..4535
                     /gene="dltD"
                     /locus_tag="ACHA9N_002210"
     CDS             3267..4535
                     /gene="dltD"
                     /locus_tag="ACHA9N_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9085648.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4676..5758
                     /locus_tag="ACHA9N_002211"
     CDS             4676..5758
                     /locus_tag="ACHA9N_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9085649.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5758..6561
                     /locus_tag="ACHA9N_002212"
     CDS             5758..6561
                     /locus_tag="ACHA9N_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9085650.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6631..7371
                     /locus_tag="ACHA9N_002213"
     CDS             6631..7371
                     /locus_tag="ACHA9N_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9085651.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7382..8386
                     /locus_tag="ACHA9N_002214"
     CDS             7382..8386
                     /locus_tag="ACHA9N_002214"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9085652.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8431..>10254)
                     /locus_tag="ACHA9N_002215"
     CDS             complement(8431..>10254)
                     /locus_tag="ACHA9N_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085653.1"
                     /translation="YNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMK
                     EIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQW
                     ITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKV
                     YLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFA
                     LALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCT
                     FQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTP
                     WIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQV
                     DFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILE
                     EAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIH
                     ALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQE
                     RFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3291 a         1700 c         2075 g         3188 t
ORIGIN      
        1 ctatgtgaca aaactgttag taaaaaaacg gtttctgtaa gctagacaca tgtttctttt
       61 tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa tgaacaaatg
      121 gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa ttttttatag
      181 tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag gaggaacttt
      241 tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt gaagcaatcg
      301 atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag acgcatactt
      361 ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa aaggtagaag
      421 gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc tcatttctag
      481 gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc aaggagcgta
      541 tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg gactggatag
      601 aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc aagaagatac
      661 cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat attattttta
      721 cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat ctagtcagct
      781 ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc ttagcccaag
      841 cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt tctggcggaa
      901 tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc gcgacattgc
      961 cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt tgcttgatgg
     1021 aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt tgcggagaag
     1081 aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca cggatatata
     1141 atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt gaccagcatg
     1201 tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg aaagtttcta
     1261 ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt attgcaggac
     1321 caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg ttcttttgtc
     1381 aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa tatgggcttt
     1441 tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg attgaactcg
     1501 gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc gtcgtaccaa
     1561 aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa aacaaaacga
     1621 aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa gttgtaatgg
     1681 attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca gttaatggaa
     1741 aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac ctcatatgat
     1801 cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac cgatcgttct
     1861 atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga cacttttctt
     1921 tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga ttgggtatgt
     1981 tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga aaaatcagac
     2041 aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa cgaaagtgac
     2101 tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct cttatttaac
     2161 ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa catataagcc
     2221 atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac caatcgatcg
     2281 ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat atgttgatct
     2341 tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca ttatcggtta
     2401 ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc atggtggtat
     2461 ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt ttgattttgc
     2521 tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga caccaatcaa
     2581 tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt ggcatatgac
     2641 gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca tgatgaagaa
     2701 aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt tgtttcttct
     2761 aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt atcatgcttg
     2821 tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag agcagttgcc
     2881 ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag tatgttttag
     2941 cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa tagtgacata
     3001 ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc aatcttagaa
     3061 gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt tgatgaaggg
     3121 ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga gctggatatc
     3181 caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa aatcattcaa
     3241 caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt ttcggcccgg
     3301 ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa gtagatatct
     3361 ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg ttgaaaggag
     3421 atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt ggctcaagtg
     3481 aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac cagcgaaact
     3541 atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat atgatgatgc
     3601 gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct cctcagtggt
     3661 tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa cttcaaacat
     3721 acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta gctagaagat
     3781 tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg caaacgatca
     3841 aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa tggaacatgc
     3901 tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag caaaaaatcg
     3961 atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc attttagcaa
     4021 atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac gatttttata
     4081 cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat tgggattatc
     4141 gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca aaaaaccata
     4201 acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat accggactat
     4261 ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat agccaaggat
     4321 tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg gaagacacga
     4381 tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg tttttagaag
     4441 aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc aaatcttggc
     4501 aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt gttgctcgct
     4561 tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt tgcctgaaca
     4621 aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa aacatttgga
     4681 tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac ctggaaatga
     4741 agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg atatttttta
     4801 tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca aggtctttat
     4861 ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa aaggattctt
     4921 ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc aagttgaaat
     4981 caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac cacatgtttt
     5041 aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg atattggcgc
     5101 atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg tcactccata
     5161 tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat gggataatcg
     5221 tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag cacacccaaa
     5281 tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg ctcgaacaag
     5341 tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag caggagacac
     5401 tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac agattttgat
     5461 ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg atgtagcaga
     5521 ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg atgcaggaca
     5581 aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg ttcgttatct
     5641 gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga aactagtcac
     5701 agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt atgaataatg
     5761 aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt ggaaaatcaa
     5821 tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt catccagaac
     5881 ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt attgagtttt
     5941 ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat tgcgctagta
     6001 gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca aagaaagtat
     6061 tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct actcaacagt
     6121 ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca gctgcttttc
     6181 agttcaaatg cggtacctac gggtgtcatg ccagaagatt ttcttgatat cagctttgtt
     6241 ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt tggcggttta
     6301 ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat tcaatgtttg
     6361 aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt gcagctgtct
     6421 tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt gtgggtcatt
     6481 gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga aaaaggaggt
     6541 ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat aacttttgtg
     6601 gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg atgcttagga
     6661 gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga cgggttcaat
     6721 ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct tgatccactt
     6781 gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga ccttggtgta
     6841 ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct ttcaatttat
     6901 ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt ctcaaaagga
     6961 catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac ttttatggaa
     7021 acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac ggggaaagta
     7081 ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc gaaagatgct
     7141 gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca tgcccatttc
     7201 acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact tgtacttaca
     7261 cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa ggcagcaggc
     7321 aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta ggaggaagaa
     7381 catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa ttgaacaata
     7441 tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca atgaaccttc
     7501 aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt atgtggaaga
     7561 aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc atgattttgg
     7621 aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg actttgcgaa
     7681 agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg cggaattgaa
     7741 aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga tgtatgctac
     7801 caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca atgaagtagt
     7861 caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat cgatccgttc
     7921 tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga caacagaaga
     7981 atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag aagtaaaaac
     8041 gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg aatattatcg
     8101 aaattgggat tggaattacg ggaagtctcc agatttcaat ttctctcgcc aaaaacgttt
     8161 ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca aagaagcaaa
     8221 aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa aattggttgg
     8281 acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg tgaagaaata
     8341 ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat aaaaaatagc
     8401 ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat ttcgagagag
     8461 ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc ctgtgtaacg
     8521 atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta attcttctgt
     8581 gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac aaatgacgaa
     8641 atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat tttcgatggc
     8701 atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca gatcgtcatc
     8761 gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat tccgtttggc
     8821 agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc aaaaagaaca
     8881 cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt tagcttttgt
     8941 ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt ccaggcgaag
     9001 tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca acaaatcatt
     9061 tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga tgtgatcgat
     9121 tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag accaattgat
     9181 cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga taatggctac
     9241 caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg cttcgccaat
     9301 gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa tcggcattaa
     9361 ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa aaatcaaaag
     9421 ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc tccaatagta
     9481 gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag aatgaacgaa
     9541 tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc ctaatgccaa
     9601 agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat acatcaagta
     9661 gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg tcaatacttg
     9721 cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta gataaacttt
     9781 cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca atttttttcc
     9841 gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc tttcttcctg
     9901 ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc attgatggtt
     9961 tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt ccataatgaa
    10021 tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa acttaaaatc
    10081 aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt gattgctgtc
    10141 cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata acaatgataa
    10201 tataatgatt atgctccact ttatacgtaa ataaaaagat tcttttttat tata
//
LOCUS       DAYYJC010000089        10195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000089 DAYYJC010000000
VERSION     DAYYJC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.091"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACHA9N_002216"
     CDS             120..626
                     /locus_tag="ACHA9N_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9085654.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACHA9N_002217"
     CDS             643..1542
                     /locus_tag="ACHA9N_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9085655.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACHA9N_002218"
     CDS             1629..2513
                     /locus_tag="ACHA9N_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9085656.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="ACHA9N_002219"
     tRNA            complement(2705..2776)
                     /locus_tag="ACHA9N_002219"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA9N_002220"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="ACHA9N_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9085657.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="ACHA9N_002221"
     CDS             4319..5410
                     /locus_tag="ACHA9N_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9085658.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="ACHA9N_002222"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="ACHA9N_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9085659.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA9N_002223"
     CDS             6562..7752
                     /gene="pgk"
                     /locus_tag="ACHA9N_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9085660.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA9N_002224"
     CDS             7856..8611
                     /gene="tpiA"
                     /locus_tag="ACHA9N_002224"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9085661.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="ACHA9N_002225"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="ACHA9N_002225"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9085662.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3566 a         1886 c         2112 g         2631 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cagat
//
LOCUS       DAYYJC010000090        10125 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000090 DAYYJC010000000
VERSION     DAYYJC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.092"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..977
                     /locus_tag="ACHA9N_002226"
     CDS             198..977
                     /locus_tag="ACHA9N_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085663.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1023..1967
                     /locus_tag="ACHA9N_002227"
     CDS             1023..1967
                     /locus_tag="ACHA9N_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9085664.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1983..2762
                     /locus_tag="ACHA9N_002228"
     CDS             1983..2762
                     /locus_tag="ACHA9N_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9085665.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2774..3472
                     /locus_tag="ACHA9N_002229"
     CDS             2774..3472
                     /locus_tag="ACHA9N_002229"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9085666.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3500..4264
                     /locus_tag="ACHA9N_002230"
     CDS             3500..4264
                     /locus_tag="ACHA9N_002230"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9085667.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4308..5759
                     /locus_tag="ACHA9N_002231"
     CDS             4308..5759
                     /locus_tag="ACHA9N_002231"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9085668.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5791..6927
                     /locus_tag="ACHA9N_002232"
     CDS             5791..6927
                     /locus_tag="ACHA9N_002232"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9085669.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6957..8033
                     /locus_tag="ACHA9N_002233"
     CDS             6957..8033
                     /locus_tag="ACHA9N_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9085670.1"
                     /translation="MHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKSDYDEEIISLG
                     GKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKAAKKAGIPIRI
                     AHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKFTLIPNAVDAK
                     KFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFHAIKNNSVLLI
                     IGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLFEGVPLVGIEA
                     QFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKAD
                     YNIQTAYKILESKYYELFELIQRG"
     gene            8039..9061
                     /locus_tag="ACHA9N_002234"
     CDS             8039..9061
                     /locus_tag="ACHA9N_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFM9085671.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9083..>10125
                     /locus_tag="ACHA9N_002235"
     CDS             9083..>10125
                     /locus_tag="ACHA9N_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9085672.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKL"
BASE COUNT         3698 a         1433 c         2092 g         2902 t
ORIGIN      
        1 tgttttattt acttttaaat cgtaaattgc cctagtcaag aaaacagttt gtggtatgat
       61 tatataggga aaacttgttt acaaatattt ctgtgaatat attagtttaa ttatgttttt
      121 ttaaataaaa agctgtcatt tttatgtaag tgaattatat cttaatttat ctatcatcaa
      181 tacttaggag tgaatctatg gaaaagccat tcgtcaagtc attgactgct gtggcggtag
      241 gtgcaggtgc tattgcgatc tgtttgtttg gataccatat caataatcag cggcaacatc
      301 agcaacggat caattatgca gaatctgcca tcaccaatca aaaagacacc ttgacttctt
      361 tatcaaaaga agtagacaag ctgtatagta caaaagaaaa aatttttcta aaccctgaaa
      421 taacagaaga aacaatcaca agtttgtcaa ataagctatc ctctgtcaaa ctttcagcag
      481 atgattacga catcaaagaa tcagaattgc ctaaagaagc tgttgcaatc caagatgaga
      541 agaaagcaat cgtgacacag ttagatagtg cagaatcaaa actgaagatc cagacagctg
      601 tcaacgattt atttactaaa aatgtatcaa actggcagca agctgtagac gatgtcatca
      661 tcaaagaaaa ggtgacaagt gcagatgtcg ctcgtgtacg tgaaaatatg agctttttta
      721 aagatagtcc ttggaaaacg gttgttatgc aatatctagg attcgctgat attcagatcg
      781 cgcaagtcac gcaactagat cagttattcg atacgatgtt aaaggatggt caagtgactg
      841 ccaccgctac ttacgatcaa tatttgacgg tgctcagtca aatagagcag atccggaatg
      901 aaaaaataag cgcagcatac gcaacaaaag cacaaactgt tgctgagcaa atgggctata
      961 gcaatataag ctattgacag ttaaaacgtt gattcaagca aggttagcaa acggaggaaa
     1021 ctatggcaag acgaagaaga aaatcaagag ggaaaaaaat atttctgatt attttagcag
     1081 ttcttatcgt attagtatta gcaattgtag gagtaggagc aaaattatat ctggatctct
     1141 cacattctat tgagaaaact tacgaatcag tggaacgaac ccaaggagaa cgaacgaaac
     1201 cagttgatct ggacaaacaa gaatctttct ctgttctatt gatggggatt gatactggag
     1261 atttaggtcg ggtcgatcaa ggaagatcag atacgatgat ggttgccaca gtcagtcctg
     1321 aagacaaaca gacaacgatc gtcagcattc ctcgtgatac gtatgtcgac atcgtaggtc
     1381 gtgacacaaa agacaagatc aaccacgctt acgcattcgg cggagctgcc atggcgatgg
     1441 ataccgtaca aaaatatgta gacatcccaa tcgatcacta tgtctctatc aatatgaaag
     1501 ggctaaagga actcgttgat gcagttggtg gtatcgaagt caataatgac ctcactttct
     1561 cacaagatgg ctatgacttt ccaatcggta aaattacatt gaacggggat caggcattat
     1621 cttattctcg tatgcgttat gaagacccaa atggtgatta cggtagacaa gggcgccaaa
     1681 gaaagatcgt agaaggcgta gcgaaaaaag tattgagtct aaacggtgtc agcaactatc
     1741 aatccgtttt gaatgctcta gagtcgaata tgaaaacaga catgaacttt gatgaaatga
     1801 aaaaaattgc tttagattat cgggaggcat ttggtacgat taagcaagat cagctgcaag
     1861 gagaaggctt catgcaagat aatatctcct atcaaaaagt cagtgatcaa gaacttgatc
     1921 gtgtgaaaaa agaattaaaa gatcaaatga atctagagaa taaataggaa agtggagaaa
     1981 aaatggaaga gacaatcagc ttgaaagaaa tttttgaaac attacgcaaa cacttggtaa
     2041 taatcatcat ctcaatgttt gctggcttgg ccatctcagg aatcgtaaca tttttcatct
     2101 tgacaccaaa atacagttca caagcacaat tgatcgtgac actgccacaa tcagacactg
     2161 caaatgtcaa tgatgtcaat acgaacctgc aaatgatcaa tacgtataaa gatatgatcg
     2221 tcagtgattt agtgctcaat gaagtcaaag atcgtttaga aacagaagac aatgtgaaaa
     2281 tgactgctga acaaatcaaa gatgccatct cagttaatca ggcagaaaat tcacaaatgt
     2341 tctcgattca ggcaataagt acgaatgcag tggctgctca acagatcgct aacacaactt
     2401 cacaagtttt ccaagagaac gcaaaagatg tgatgaatgt tgataaaatc tcgatcattt
     2461 caggtgcagt tgccaatacg acacctgttt cgccaaataa taaattgaat ttaatgactg
     2521 gcttaatgtt aggaatgatg gtaggtgtag gtctggcatt cttattggaa ttactcgatc
     2581 ggacagtgaa agatgaaaaa tatgtgacag atacattagg attcccaatc ttaggaacag
     2641 ttccagaaat gtcagcgaaa gaattgaatg ctggtatccg taaaaaagga ccagctcaga
     2701 cacaaccaaa agctacaaac gataaaacag cgccatcacg cagaagccgc tcaagagtct
     2761 aggaggaaag aaaatggcac gaacacagaa acaaaaagca gcccaaactc atgcagtcag
     2821 tttggtcaca ctcaccaatc cctcttcacc aatcgctgaa caatatcgta cgattcggac
     2881 gaatatccag tttgcctccg cagctggtca gcaaatcaaa acgattgttg tgacttcatc
     2941 aggacctgga gaagggaaat cgaccactgc agcaaacatt gccgttgttt ttgccaaatc
     3001 tggtcaacgg gtgctgctag tcgatgcaga cttacgtaag cccgtcatct ataaaacgtt
     3061 caagttgaac aacgcaagcg gattaagtac tgttcttagt tcatcaggaa gtgtagcaga
     3121 tgagatccaa cgaacaccag tcgacaattt gtcgattctg ccaagtggac ctaaaccacc
     3181 aaatccatca gaactactaa gttcgccaag aatggatcag attttagcag aagcaagaca
     3241 actatttgat gtagtgatct tcgacatgcc gccagtcgtt gccgtaacag atgcacagat
     3301 catgtcttca aaaacggatg gcaccttatt ggtcgtacgg gaaaatacgt cacgaaaaga
     3361 atcattgact aaagcaaaag aactgttaga tatggtacaa gcacgagtct taggtgtcgt
     3421 atacaacgga gcagaacaca gcaaagatgc tggttattac tacggaaatt aaggaataag
     3481 aagagaggag acaagagaaa tgatcgatct acattgccat atcctaccag gaatcgatga
     3541 cggggcagaa aacttagaag caagtattgc aatggctgaa aaagcgattc agcaaggcat
     3601 tacgcatatt ttatgcacac cacaccacaa taatggaaaa tacagcaatg aaaaatcaca
     3661 agtcatttct ctagtcgctt cgttacaagc cgaattagag aaaagacagt tgcctttgac
     3721 gttattggaa ggacaagaag tacgtattac cggaacgctc atcgaagaca tccaccgaga
     3781 tgaaatttta tttacagatt tagatgatac atatcttctg atcgaatttc cgacactgga
     3841 agtgccgtta tatgcagaac ggcttttttt tgagttgtta gaactaggaa agataccagt
     3901 gatcgtccat ccagaacgca acgcgcattt tcgagaagac ccaaatcatt tgcttccgtt
     3961 cctagatatg ggatgtttgg cccaacttac tgcgccaagt tatgttggtg tatttggcaa
     4021 agatatccag aaaacagcca aaacaatggt caaacataat ctcgtgcaga tggctgcttc
     4081 tgatgcacac ggggtctcta agcgcacctt ttacttaaaa gagtgctatg agcagattgc
     4141 caaggagttt ggtaaggaaa aagtagtaca gatgaaacaa gtcgcaaagg atctgatcaa
     4201 cggggatgag atcaattatc caatctatga agaggtaaaa aagaagaagt ttggtttatt
     4261 ttagttcttt attcgtagat aaaaaggaaa tgaggagaga gtgagagatg tatagaaaaa
     4321 actcaagtgg ttggtctaaa catattgatt ttattttatt agacttattt tgtgtccagc
     4381 ttgcttttta tatatcatat gtaatgcgac agggtgactg gaatccgtat gtgattccta
     4441 tttatcgcca catggcaatt tttatagaac ttgagaatct tacgattata tttttatttg
     4501 aggcatataa aaatgtttta aaaagaggat attataaaga ggctgtagct tctataaagc
     4561 aatcttttat gctgttactt gtgtgcagtt tataccttat tacaatgcag gatggaaatg
     4621 attattccag agtttcttta tttataatgg gggtggtgta tggattatta acatatataa
     4681 ttcgtatact ttggaaaggg ttgttgcatc accgcatgaa aaacggtgaa gatgtatcgt
     4741 tagttattgt aacatctcaa aagattgcaa aaaatgtagt tcgtaatctt aaagagaaga
     4801 attatggcat gtataaactt agtggtgtga ttgtagtaga cagagatatg tatggaacta
     4861 ccatcggagg tgtcccagta gttgcaaatg ctgataatgc agcggaatat cttttacaga
     4921 attgggtaga tgaagtattc atcaatgttg acgaaagtgt tccatatccc aaagaattaa
     4981 tcgccagatg ttcggaaatg ggacttgctg tacatatgaa tcttacaaaa gtgtctgata
     5041 gtacacgtgg aaaacaattt attggccgag ttggtgatta tactgttatt acatctagta
     5101 ttaatgttat gacgatgagg caagcattta taaagagaac gattgacatt cttgctggaa
     5161 ttattggatg tattgcaaca ggtgttattt ttatcttcgt agcaccagtt atttacatca
     5221 aatcaccagg gccgattttc tttgcacaag aacgtattgg tcaaaatggt aagacattta
     5281 agatgtataa gttccgtagt atgtatatgg atgcagaaga acgcaaagca gagttgatga
     5341 aagaaaataa gatgagtaat aatctcatgt ttaagatgga ttttgaccca agaatcattg
     5401 gcaataaaat acttcctgat ggaacaaaga agacaggaat tggacaattt attcgatcta
     5461 catcattgga tgagtttcca caattttata atgtcctaat gggaaaaatg agcctggtgg
     5521 ggtttagacc ttgcttgaag tcagaatatg agaagtatga ttttcatcat cgtgcgagaa
     5581 ttgcgatgaa acctgggatt actggaatgt ggcaggtgag tggtagaagt gatatcacag
     5641 acttcgaaga ggttgtaaaa cttgatgcag aatatatcag gaattggagt atgggattag
     5701 attttagaat tttgtttagg acagtcaaaa ctgtgcttca tagaaacggt tccatgtaag
     5761 ataaagatac tatagaagga gtaaaacgtg atggcaagaa aaaaattaaa aatctgtatg
     5821 ctaggtcata aaagaattcc atcaagagaa ggtggaattg agatagtggt agaagaattg
     5881 gcaacgcgaa tggttgcact tgggcatgaa gtgacttgct ataatcgaag tggtcatcat
     5941 gttagcggaa aggaatttga ccagcataaa aataaagaat ataaaggtgt aaaattaaag
     6001 actattttta cattaaatat aaaaggaatt gcagcaatga gttcttcagt atttggtgga
     6061 attagagcag cattcggaaa atatgatgtt gttcattttc atgcagaagg accatgtgct
     6121 atgttgtggt tgccaaaact ttttgggaaa agatgtgttg caacaattca tggtctagat
     6181 catcaaagag aaaaatggaa caagcttgct agtacataca tcatgttagg ggaaaagtgt
     6241 gctgtaaaat ttgcagatga aataattgta cttagtgaaa gcgtccaaaa ttattttgaa
     6301 gatatatatg gaagaaaaac aaggtttata ccaaatggcg ttaaaaaaat tgaaataaaa
     6361 tcagcaggat tgattacaga aaagtatgga ttaacaaaag attcttatat tttgtttttg
     6421 gggcgattgg ttcccgaaaa aggaattcgg tatctaattg aggcatttaa agatgttcag
     6481 acagataaaa aacttataat agccggagga agctctgata cagatgaatt tgcaaatgaa
     6541 ctaaaggaat tagcaaaagg tgatgagcga attatattta ctggatttgt acaaggacag
     6601 gaattagaag aattatatag taatgcgtat atctatacac ttccaagtga tttagaagga
     6661 atgccattaa gtttgttaga agcaatgagt tatgggaact gttgtatagt ttcaaatatc
     6721 tcagagtgta cggaagtagt agaagacaag gctatgattt ttaagaaatc ggatgtaagt
     6781 gatttaaaga taagattaga agaggcatgc aatcaatcgg aaatggttaa agttttgaag
     6841 aatcaagcta ctgagtttat ttgtagtaaa tataattggg ataaaatcgt acaagaaaca
     6901 ttaaatttgt ataggggaaa tatataatga ttcgaatatt acagtgtgtg aatgatatgc
     6961 atcgtgcagg gttggagacg atgctaatga actattatag gaatatagat cgaactaaaa
     7021 ttcagtttga ttttttgaca catcgtccat ataaaagtga ttatgatgag gaaataataa
     7081 gtcttggagg aaaggtctat tatgcaccga gactttatcc acaaaattat cctaaatatt
     7141 ttaaatggat ggaacaattt tttaaggatc atccagaata tgagattgtt cattctcata
     7201 ttgatgcgat gagttattta cctttaaaag ctgcaaaaaa agctgggatt ccgatacgga
     7261 tagcacatag ccataataca tcgatagata gagattttaa atatctttta aagcaatatt
     7321 atcgatgtaa gttaacgtct gttttgacac atgaattttc atgtggaaaa gtagcaggtg
     7381 attttttgtt tcgaaatgat aaattcactt tgattccaaa tgcagtggat gctaaaaaat
     7441 tttattatga tgaaagtatt agaaatgatg tacgaatgaa attaggaatt tcaagtcaga
     7501 cgtttgtaat gggacatatt ggaagaattt catattctaa aaatcatcgt tttttgattg
     7561 aaatttttaa agaatttcac gctattaaga ataattccgt attattaatt attggaactg
     7621 gtgatatgga agaagaaata aaaaattatg caaagaaaag tggcatagat gatgatatta
     7681 aatttttagg aaatagaaat gatatagaaa aattttatca agcatttgac gtattaatgt
     7741 tgccatcatt atttgaaggg gttccgttag tgggtattga agctcaattt gcagatttac
     7801 cgtgtttctt ttcggagaaa gttcctacag aagttgcttt cagtgataag accaatttca
     7861 taagtttaaa tcaatcggca aaagaatggg cgagagagat tgcaaatgta gatatctctc
     7921 atcgagattc agaacgttcg attttaataa aggcagatta taatattcaa acagcataca
     7981 aaatcttaga atcaaaatat tatgagttat ttgaattaat acaaagaggt taaagaatat
     8041 gacacagaaa cattcaagta taaaaagaat tatacatcgc atatccgaag aattgaatgc
     8101 aacaagacga attgcaaaga taacatcttg gaaggaagca tttgccacgt tacaagctaa
     8161 aattgatatt caagtcatga atcgtaatgg atataatgaa agtaaaaaac agaaggaaca
     8221 tttattaaaa aaacatgagg taatgctgaa gtattatgaa aagacttttt cagatttttt
     8281 gaaaacatat gattatagtc aaaatcaaat aattaatcaa tcaaaatcac catattctaa
     8341 ctgcatttgg gtttgttggt ggcagggatt ggatcaatcg ccggaaattg taaaagcttg
     8401 tgtaaaatca ataaaacgaa atgcagggaa acatactgtt ataattttaa cagaagataa
     8461 ttatagacaa tatgttgata taccaaaatg ggtggaagaa aagaaaaaca aaggaatcat
     8521 tacgagaaca aattattctg atttattaag attatctttg ctagcaaaac atggaggttt
     8581 gtggttagac gccacatttt attgtacaaa accaatacca gatgagtatt ttgatatcga
     8641 tatttggtct attaggaggc ctgattatgc tcacgcatcc gtagcatcag gatattttgc
     8701 gggttattca ttagcatgta atcaagagtc tagatggatg tttgctataa ttagagactt
     8761 ttttttaaat tattggaaaa ataatgatat gatggtggat tatttgatga tagattatat
     8821 gattgtttta gcacagagaa taaatcatga aatagcacag acatttgata aaatacctag
     8881 taacaatcca ttatgtgacg aattgattaa aaatttaaat aatatatatg atgaaaaagt
     8941 atggaactta ttaaagaaag atactttact gtttaagttg acatggaaac aatcttttaa
     9001 aaagcaaaaa gatgataaag ttacttttta tgggatgttg gtagaaaaca aattattata
     9061 aatattagag gtataaaaag ttatgaaaca caaggtaagt gtgatagtac caatttacaa
     9121 tgttgaaaaa tatttaagaa aatgtgtgga tagtttattg agtcagacat taaaagagat
     9181 agaaatcgta ttagttgatg atggttcgcc agatgcttcg ggggaaatag cagatgagta
     9241 tcaaaaaaaa tattctaatg taaaaacaat acatagagaa aatggggggt taggaccggc
     9301 aagaaataca ggaattgaga atgcgacagg agaatatgtg gcttttcttg attcagatga
     9361 ttgggtagaa agtgatatgt atgaaaaatt atacttagtt gcgtccaaag aaaatgctga
     9421 tatagtagtt agtggacatt gtgattttgc gaatgacaaa gctatggtga aaaagcaaca
     9481 tcctttgagt gggaatgtat atgatacgcc taatagtatt aaaaatgtac gaaaaaattt
     9541 atttggacat agtccaaaag atcaagaagt agaggccttc ccgatgtcag tatgtatgtc
     9601 tttgtataat aaaaatctgc tagataaata tagactaaga tttgaaaaaa ttttatctga
     9661 agatacaatt tttaacttga atgcatatga tgtcgcaaat aaaattgctt ttacagaata
     9721 tacagattat tgttatagaa aagaagaaca agagtctatt acaaaaagtt tatctccaaa
     9781 taagcagacg gaatttaaag aatttttaat aactctaaaa gctatagcat cgaaagaaga
     9841 tttagaatgt caaatcagag caaaacgtat ggcaattgat tattgtaggc tttatgtggg
     9901 cttagttgat gatgcaaatt tgggaattaa agagaaaaga aagtatataa aaaaatttat
     9961 tgaagatgag ggattaattc aatgttggaa gggatatcct acaaatacgc taccatttca
    10021 acaaaaattt tttcataaaa tgcttgaatg taagtgttat acaatattgt tgttgttaag
    10081 tagaattaga aagataatta aaaatatgac cagaaaaaaa ttgtt
//
LOCUS       DAYYJC010000091        10084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000091 DAYYJC010000000
VERSION     DAYYJC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.093"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHA9N_002236"
     CDS             162..392
                     /locus_tag="ACHA9N_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9085673.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHA9N_002237"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHA9N_002237"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9085674.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHA9N_002238"
     CDS             1803..1979
                     /locus_tag="ACHA9N_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9085675.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA9N_002239"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA9N_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9085676.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="ACHA9N_002240"
     CDS             2474..2941
                     /locus_tag="ACHA9N_002240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085677.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="ACHA9N_002241"
     CDS             3026..3910
                     /locus_tag="ACHA9N_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9085678.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="ACHA9N_002242"
     CDS             4024..4260
                     /locus_tag="ACHA9N_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9085679.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="ACHA9N_002243"
     tRNA            4348..4428
                     /locus_tag="ACHA9N_002243"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="ACHA9N_002244"
     tRNA            4435..4506
                     /locus_tag="ACHA9N_002244"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="ACHA9N_002245"
     CDS             4631..4801
                     /locus_tag="ACHA9N_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085680.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="ACHA9N_002246"
     CDS             5021..5728
                     /locus_tag="ACHA9N_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9085681.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="ACHA9N_002247"
     CDS             complement(5788..6432)
                     /locus_tag="ACHA9N_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9085682.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="ACHA9N_002248"
     CDS             6559..6846
                     /locus_tag="ACHA9N_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085683.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="ACHA9N_002249"
     CDS             6954..7274
                     /locus_tag="ACHA9N_002249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9085684.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="ACHA9N_002250"
     CDS             7355..8668
                     /locus_tag="ACHA9N_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9085685.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="ACHA9N_002251"
     CDS             8665..9384
                     /locus_tag="ACHA9N_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9085686.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="ACHA9N_002252"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="ACHA9N_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9085687.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DAYYJC010000092         9974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000092 DAYYJC010000000
VERSION     DAYYJC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.094"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(438..662)
                     /locus_tag="ACHA9N_002253"
     CDS             complement(438..662)
                     /locus_tag="ACHA9N_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085688.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            853..3630
                     /locus_tag="ACHA9N_002254"
     CDS             853..3630
                     /locus_tag="ACHA9N_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9085689.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3711..4130
                     /locus_tag="ACHA9N_002255"
     CDS             3711..4130
                     /locus_tag="ACHA9N_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9085690.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4142..4612
                     /locus_tag="ACHA9N_002256"
     CDS             4142..4612
                     /locus_tag="ACHA9N_002256"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9085691.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4629..5408
                     /locus_tag="ACHA9N_002257"
     CDS             4629..5408
                     /locus_tag="ACHA9N_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9085692.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5395..6216
                     /locus_tag="ACHA9N_002258"
     CDS             5395..6216
                     /locus_tag="ACHA9N_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9085693.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6233..7282
                     /locus_tag="ACHA9N_002259"
     CDS             6233..7282
                     /locus_tag="ACHA9N_002259"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9085694.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7295..8299
                     /locus_tag="ACHA9N_002260"
     CDS             7295..8299
                     /locus_tag="ACHA9N_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9085695.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8932..9951
                     /locus_tag="ACHA9N_002261"
     CDS             8932..9951
                     /locus_tag="ACHA9N_002261"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9085696.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3349 a         1646 c         2088 g         2891 t
ORIGIN      
        1 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
       61 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      121 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct
      181 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      241 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      301 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      361 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      421 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      481 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      541 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      601 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      661 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      721 aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat
      781 aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat
      841 gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag
      901 acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat
      961 cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa
     1021 ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa
     1081 caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag
     1141 ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg
     1201 aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt
     1261 ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat
     1321 tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa
     1381 tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac
     1441 actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt
     1501 ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat
     1561 caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc
     1621 ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata
     1681 cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg
     1741 atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga
     1801 ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat
     1861 atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat
     1921 gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca
     1981 cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag
     2041 cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat
     2101 aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat
     2161 tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa
     2221 tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac
     2281 agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa
     2341 gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca
     2401 tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa
     2461 gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa
     2521 gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg
     2581 gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta
     2641 gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt
     2701 tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt
     2761 aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc
     2821 acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt
     2881 cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc
     2941 attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa
     3001 agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat
     3061 aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca
     3121 gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag
     3181 atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc
     3241 aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta
     3301 gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg
     3361 aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt
     3421 gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag
     3481 tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat
     3541 agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca
     3601 gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg
     3661 catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag
     3721 aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga
     3781 tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt
     3841 tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga
     3901 cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg
     3961 tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct cggttggacg
     4021 ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc
     4081 agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa
     4141 aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg
     4201 gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga
     4261 gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc
     4321 aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat
     4381 cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac
     4441 catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa
     4501 tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga
     4561 aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa
     4621 gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag
     4681 aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa
     4741 tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta
     4801 tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag
     4861 ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc
     4921 cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg attccaatta
     4981 tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc
     5041 acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata
     5101 tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg
     5161 ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc
     5221 tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag
     5281 agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata
     5341 ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag
     5401 aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt
     5461 tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat
     5521 gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg
     5581 aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc
     5641 acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat
     5701 agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt cttttgggga
     5761 actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta
     5821 ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca
     5881 acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg
     5941 atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact
     6001 gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact
     6061 gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata
     6121 gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc
     6181 tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac
     6241 gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga
     6301 tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc
     6361 atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt
     6421 cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac
     6481 tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat
     6541 acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt
     6601 agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa
     6661 aggctattct gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa
     6721 aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa
     6781 ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt
     6841 tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga
     6901 agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc
     6961 ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg
     7021 ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg
     7081 agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat
     7141 agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta
     7201 taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac
     7261 agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca
     7321 aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa
     7381 actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc
     7441 ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca
     7501 tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt
     7561 gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt
     7621 aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga
     7681 ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga
     7741 tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga
     7801 acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc
     7861 atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg
     7921 gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa
     7981 atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc
     8041 agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact
     8101 tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg
     8161 tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca
     8221 acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta
     8281 tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag
     8341 cgataactgc tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt
     8401 ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca
     8461 ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt
     8521 acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag
     8581 ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa
     8641 atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc
     8701 tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa
     8761 gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt
     8821 aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa
     8881 gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa
     8941 aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa
     9001 aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag
     9061 tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat
     9121 aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa
     9181 gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata
     9241 catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa
     9301 gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag
     9361 gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca
     9421 gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg
     9481 attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc
     9541 catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa
     9601 atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt
     9661 gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac
     9721 ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag
     9781 ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca
     9841 attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa
     9901 gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat
     9961 caatagttaa tcgt
//
LOCUS       DAYYJC010000093         9915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000093 DAYYJC010000000
VERSION     DAYYJC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.095"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..588)
                     /locus_tag="ACHA9N_002262"
     CDS             complement(76..588)
                     /locus_tag="ACHA9N_002262"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9085697.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(602..2899)
                     /gene="recJ"
                     /locus_tag="ACHA9N_002263"
     CDS             complement(602..2899)
                     /gene="recJ"
                     /locus_tag="ACHA9N_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9085698.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3041..3484)
                     /locus_tag="ACHA9N_002264"
     CDS             complement(3041..3484)
                     /locus_tag="ACHA9N_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9085699.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3485..4273)
                     /locus_tag="ACHA9N_002265"
     CDS             complement(3485..4273)
                     /locus_tag="ACHA9N_002265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9085700.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4270..5211)
                     /gene="rnz"
                     /locus_tag="ACHA9N_002266"
     CDS             complement(4270..5211)
                     /gene="rnz"
                     /locus_tag="ACHA9N_002266"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9085701.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5388..5975)
                     /locus_tag="ACHA9N_002267"
     CDS             complement(5388..5975)
                     /locus_tag="ACHA9N_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9085702.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6196..7509)
                     /gene="obgE"
                     /locus_tag="ACHA9N_002268"
     CDS             complement(6196..7509)
                     /gene="obgE"
                     /locus_tag="ACHA9N_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9085703.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7797..8807
                     /gene="gap"
                     /locus_tag="ACHA9N_002269"
     CDS             7797..8807
                     /gene="gap"
                     /locus_tag="ACHA9N_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9085704.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8892..9827)
                     /locus_tag="ACHA9N_002270"
     CDS             complement(8892..9827)
                     /locus_tag="ACHA9N_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085705.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2726 a         2270 c         1715 g         3204 t
ORIGIN      
        1 gagaatctga cttttgaggg tcgctttaaa atcgcttctt taagaataac atcaagaata
       61 gcttcttttt tctgcttaat attccattaa tgtaatgatg tcatatcctt tgattttgtc
      121 gcgtccatgg agatccatca attcgatcaa aaatgcgcag cctacgacta caccgcctaa
      181 ttgttcgatc agttcgatcg tcgctttgat tgttcctcca gtagctagca aatcgtcaca
      241 aatcagcaca cgttgacctg gtttaatggc atccttatgc aatgtcaatg tatctgttcc
      301 atattctaat ccataagtca cttcgatggt ttctcgaggt aacttccctt ttttgcggac
      361 tggggcaaaa ccgactccta actcataagc aacaggacat cctacgatga atccacgggc
      421 ttctggccca actaccatat caattcgttt ttcttttgca taatttacga tttgtttagt
      481 agcttcccga taagcgtcac catctgccat cagtggggaa atatctcgaa acacaattcc
      541 ttttgacgga tagtcgggaa tactggcaat ataatctttt aaatccactc tttgtttcct
      601 cctaggttgt taaccatttc tttatggtag gaatatcgct caaaagcaaa aattcctcaa
      661 ctttgatttt attgagccgt cgctggtata atctgctctc agtcaaagga tgactttctg
      721 ggctagcgac tttttgtaaa acaccgttct ctattgtaac aaatttcaat tcaaaaaaca
      781 cctgtatcat aaaaattaat aatttttcag ggatacgcaa atattgagct attgctttta
      841 ttttatgacg tatatcaatt cgttcttgag aatggatcaa tttaaacaat tttgcgtact
      901 gttctcttgt gcctacccca ttcaaatagg cctcatcagg tgaaatcccc atcagataaa
      961 cacgattgaa tgaataagac cgaaaaactt ctttcagatc cgctaaatca tctggacaat
     1021 ccataacaac tagagagttt ggttttcttt tttcaattat tgcctttaca gaatctagtc
     1081 cttcgaaaac aatcgcagct tcctggataa tctcaggcac gaatttcaac gtcgaagaat
     1141 caaatgctaa ataaaggcta ccttccgacg ttcggctctg ttcacggaaa cgttttgccc
     1201 gccaatcaaa tacttgcaga ccacccacag aaaaatcaga taccatcagc tgcggtttct
     1261 tacgcccgtt ccattcattg atcgaaagct ttcctaccac atctacctga ttattcagca
     1321 gttcttcttc ttgagtacca aaaccaaaag cgaccgcatc aagctgactc gatgcatcgg
     1381 aaaggacaaa tttcagatgc tgttggtttg ctccgattct tttgatattt tctgcttgga
     1441 cttgtcgaaa taaaaactga ggcaaaggat tatccgtacc aaatggcgcc aatagtttca
     1501 gctcatcaat tcgctctact gttacttcat ttggcagcaa tacttcatca attctcaact
     1561 caggccctct catcaagtca atttgatgat cgactatata ttggttcatc ttctcctgta
     1621 gtatagtgac attttcactt ggcatcgtta aaccgactgc tgcatgatgt cctccgaagt
     1681 aagtaaataa atctcgcatc tggtcaagca tttcaaaaag attcaaggct tcaatactac
     1741 gtccagatcc tttagctgat ccatcttctt ttaaagtaag gacaagcgtt ggtttacccg
     1801 tttcattcat tattttccca gcaacaatgc ccaaaacgcc ttcatgccat cctcggttta
     1861 ccaatagatg gatatgattc tccttattga ccatcgctaa tgcttcttgt gttatctgct
     1921 ctacgattgc ttttctttct tcattgatct cattcaattt ttttgcttga acggatgctt
     1981 cctcttcatc aaacgtcgca agtaacgaga cggcaggacg tggatcgccc atccgaccga
     2041 ttgcatttag tctgggagca atcgaaaaac cgatcgttgt ttcgtctgcg tccctcattt
     2101 ttacgccgct ttcttcaaat agcgcctgaa gtcctatccg ttctgaatga tgaatggcat
     2161 cgattcctaa tgaaacaagg attcgattct catcagtcaa tgaaactaaa tcggcgatcg
     2221 ttcctatcgc tactaaatcc aaaaattctg caggcggttc ttccaataat gcagaagcta
     2281 ctttgaatgc tactccgact cctgccaaat ctggaaatgg atagtgcccc gctggatgac
     2341 gagggtgaac aatcgcataa gcgtctggta aattctcagg tagttcgtga tgatctgtaa
     2401 tgatcacgtc tacaccagct tgctgagcgt atgctaccgc atcatttcct gctaccccgt
     2461 tatcaacagt gatgatcaac tggattcctt cttcgatctt ttcttggtat accgattggt
     2521 ttggtccata cccatgctca aatcgatttg gcaggaatac ttctacttct gcaccaagta
     2581 attccagtgt ttctttcatt accgttgcac tggtgatccc atctgcgtca taatcaccgt
     2641 aaacgaggat tttttctcca ctgattacag cttcttggat tcggctgatt gctttatcca
     2701 tatcgtgtat cagatacgga tcatgtaatt cttgttcctt tggatgtaaa aatcggttga
     2761 tatccttttc ctcatggtaa cctctttgcc acagtaactt accgacaaat ggagataatt
     2821 tcagttcacg gatcatttgg agaaaggatt cagaaggctc tgtctcgctt aacttccaat
     2881 caaaatttgc ttgtctcact ttctcactcc taacttagtt attatagcaa aggaaatcag
     2941 caaaaaaaag cagaaagaaa gaataaaaac agatcagatc cgctagacag atgttcagca
     3001 ggacctgatc ttttgtacga ccatttatga aatatcccta ttagtcaaaa taattgaagc
     3061 tttgtttggg cgattgagtt ttttgttctt ccggcgcttt caattgactg atttgtgcgg
     3121 ttttcgcttg taactccgcc tgataagcag cttccaactc cgcacgttcg tttctaaact
     3181 cgcgttgctg ttcttctttt gcttcttcaa ttttcttttg aagttctgtt tgatattctg
     3241 acatttcctg agtcagctgt ttgatttgct ttttttgctg ccatagtgtt gccgaagcag
     3301 tcaaaaatac aatcagtgca ccaatcagcg cagaaccgat gatcactaag atcaatggcg
     3361 cagaaaagga agtgaagcca aaacttacag gaacattttt attgttcaat acagcaaaaa
     3421 gaacaataat caaaactaaa acaaatccaa gaatcacatt gctttgcttt ttcaattcct
     3481 tcacctactt tttgttaaaa attccgctgg ctaaataatc accaatcgtt ggaaacagcg
     3541 tataaaattt tgatgcaact tctaaaataa acggttgatt gatttcccgt tttggatgtc
     3601 tcatattgcg gacgatcatt tttgccaact tggcaggttc taaaacgatt tgtccgactt
     3661 tttccaaata gcttccgctt ggatcagcct tatcgaagaa atttgtctcg attggacctg
     3721 gattcactgt cgtaacagct acgccaagtg gtttcaattc taatcgcaaa gcgttggaga
     3781 agccaaggac tgcaaatttc gtggcagaat aaaccgttga ttttgcagtt gccatttttc
     3841 cagccataga ggcgatatta atgatatgac cttgtcttct ttctgccata tcgatcgcaa
     3901 atttttgtgt caaaaccatc attcctaaga tgttgacttc aaacatatcg tatgcttttc
     3961 caaggtcgaa tgtaaggaag tcttcgaaaa tcccaaaacc cgcattgttt accagaatat
     4021 cgacgcgccc gacttcttcc atcacctttt catataattc gtccacgctt tcaggattgc
     4081 tgacgtctac cttgaaagca taagcctctt tgccgctcaa gcgtctgcac tcgtctctga
     4141 caccttctac aaatgccatt cttcgtgcgc aacttataac gattgctcct tgctttgctg
     4201 cttcataaca gatttgtgcg cccagaccgc ctgaactacc tgttactaaa acaactttat
     4261 cttctaaatt catgcctctt cctcctcgcg aaatggaatc tcaatgctat caaaatcttt
     4321 cataatcgtc gtgttttcaa acacttcttg tgcttctttt tcaagctcta aagctgcttt
     4381 tcctaaatat ctcgcactga tatgagtaag tagcagccgt tttgcctgcg cttcttttgc
     4441 aacttcagca gcttgatgag tcgtggaatg gaaatagttg tgcgccattc ttgcttcatc
     4501 tttattaaat gtgctttcat gtaccagaat atcgctattt tccgcaagca caacggaatt
     4561 atgtgttttt ctcgtatcgc ctaaaatagt cacgattcgt ccttttttat ctggacctac
     4621 gaaatctttt ccattgatcg tccgcccatc ttctagttgg atcgtttcac cttgcttcag
     4681 tttgccgtac agtggtccag ctggaatacc taattctttt agacgatcca cctgcaattc
     4741 tcctttatga tcatgttcaa ccacacgata accaaaactg tcgatcccat gatttagttt
     4801 ttttgtataa acagaaaatt gctgatcagt aaaaatcggg gctgtttcat ttagctcaat
     4861 gaatttcagc ggataactta aacgcgtttg cgaaatacct aaagagactt ttatgtattc
     4921 ctctatccct tttggcccgt aaatctctaa tggcgtatcc ccaccttgaa atgaccggct
     4981 gctgatgagc ccgggaagtc caaaaatatg atcgccgtgt aaatgtgtaa tgaaaatttt
     5041 accgattttt ctcggacgaa tagtcgttcg caaaatctgc atttgcgtgc cttcgccaca
     5101 gtcgaacaac caaacttcat tgcgctcgtc taaaagtttc aatgctatac ttgtcacatt
     5161 tctatgtttt gcaggaacac ctgcacctgt tcctaaaaat tgtaattcca taatctacct
     5221 gactttcttt aagttcctct ttaattttag ggtaaaaaga agggataatc aaccgtaagt
     5281 cgagaatggg ttcaaattaa aaataacgtt ctaaagcgat agcgttttct ttgaaaatta
     5341 ctataaaaac agcagcgaac ccgtttgaga tcctctgctg ttttttatta atttgaagat
     5401 ccagaacttt cattgtcgtt ggaagtttgc ggatgtttgt ccttccaagt cttttgtgca
     5461 gcttctttcg catgattttc agcttctttc gcatcatgca catctttttc gacatcttca
     5521 taatccaagc gagtaatttc tccgcctaat tccatcatta ccaattgatt cagtgggcga
     5581 ttgtcctctt catcagcaat caagatcagt cctgtagctc cttcaccgat tttatttcct
     5641 acaaagtcga acacggtctg ggcatctttt atttctttgg catcttgtgt cgcaccgatc
     5701 attcctccgg caaaccatcc taataaaatc cctagcggac cacctaatac accaaccaac
     5761 attccgatta atccacccgt agaagatttg ttattaccgg taaagtcgat aaaatcttct
     5821 attttgaatt gatgtgtccc attaggatca tggctgacaa ctgccatctg ttctccttta
     5881 atcgaacgaa cagcatgtaa ctttttgatt tctgaaaatg cctgatatgc ctggctttct
     5941 acttgaaaat ttaaaatgat tactcgtttg ctcattaaaa acgccccttt cataccttct
     6001 tgttatttat tttaccttta attgaaaaat aagaaaagaa aagcactctt tttttccaat
     6061 aaaatagggc tgtgacaaaa gtcgtatcac agctcctttt tccgaataaa cggcgttctt
     6121 gaagctgatc acttctgtca caacctccct taaatctgaa tctatatttt ttggtatttg
     6181 tttttatttg cttgcctatt cgacaaattc aaattcaaac tcaccgatac gtacaaggtc
     6241 gccatctttc gcaccgcgtg cccgaagagc ttcatccaca cccataccac gtaattgacg
     6301 agcaaaacgc atcactgttt catcatgatc gaaattcgtc atttggaata atttttcgat
     6361 cttctcacca gataagatcc acgttgcatc tgaatcacga tcgatttgga actcaggacc
     6421 ttctggattg aatccatagt gtacagtttc ttcttccagt tcttcttcgt acaatgggaa
     6481 ttctggcgtt acttctaaca aatctgctgt agcattcaat aacgcgtcca atccttgtcg
     6541 agtcactcca gaaattggaa atactgggat gtcatcagca aattcatctt ctttttcctt
     6601 gttcagctgt tctttgaatt tcactagatt ttccggtgca tccggcatat ccattttgtt
     6661 tgccacgatg atttgcggac gctctagcaa gcgtaagtta taagtagata attctttgtt
     6721 gatggcaaga tagtcttcat aaggatctcg tccttccatc ccactcatat cgatcacatg
     6781 taaaattact cgtgttcttt cgatatgacg aaggaattgt gtacccagcc caacaccttg
     6841 cgaagctcct tcaatcaagc ctggcaagtc tgctgcggca aaacttctgc catcacttgt
     6901 agtaaccatt cctagattag gtacgagcgt cgtgaaatga taagcgccga tttttggacg
     6961 agcagatgaa ataacggata gtaaagtcga tttcccgaca gatggaaaac ctacaagtcc
     7021 aacgtctgcc aatactttca attctagttc gatttttcgc tcttgtcctg gttcaccgtt
     7081 ttcagcaatt tccggtgcag gattccgagg agaggcaaaa cggatattcc ctcgtccacc
     7141 gcgtccacct tttgccacaa ctagtgtttg accattttca attaaatctc ctaaaagagc
     7201 accagtctcc gcatctcgga cagtggttcc ttgtggtact ttaacatagg tatcttctga
     7261 accacgaccg tgcatccctt tactcatccc attttcacct ggctgcgctt tgaaatggcg
     7321 attgaaccga aaatccatca atgtacgtaa cccttcatcg acgatcaaga tgacatcgcc
     7381 gccacgacca ccgtctccgc cagccggtcc gccgtcagga acgtattttt cgcgacgaaa
     7441 tgcaaccatt ccgtctccgc ccttgccagc tttcacatcg atcgttactt gatctaaaaa
     7501 catggacata attaagtcct cctattattc atttatttat ttgactgcct gaatttccac
     7561 aataaacaaa agtcttactt attttatcgg tgaaatctga ttttgtctat catttcagca
     7621 aaaagattgt ttaccttctt ctttttttat catttggtaa ggtttccaac cttttttctg
     7681 ctaccaagta taattttttg acaaaatatt ctttgcgtag cagaataaca gtgtattaat
     7741 tgtattaatt gaggatgccg tttcacagca cccctaataa aggaggaact tttgatatga
     7801 cagtaaaagt aggtattaac ggatttgggc gtattggccg tctggctttc cgccgcatca
     7861 aagaagtctc agatgatatt gaagtagtag caatcaacga cttaacaagt ccaactatgt
     7921 tagcacaatt attgcaattt gactctactc atggtactta tcctggcaca gttactgcaa
     7981 cagaagactc aatcgtagtg gatggcgaag caactcgtgt ttatgcagaa cccgacgcaa
     8041 gcaaaatccc ttgggcaaaa gaaaacggtg tagatatcgt actagaatgt actggttttt
     8101 atacatctga agaaaaagca aaagcgcatt tagatgctgg tgtcaaacga gtagttattt
     8161 ctgccccagc tggtgctatg aaaaccatcg tctataacgt caatgatgat acattagatg
     8221 aaaacgacaa aatcatctca gcaggttcat gtacaacaaa ctgtttagca cctatggctt
     8281 atttcttaaa taatgaattt gggatcgaag ttggtacaat gacaacagtt catgcctaca
     8341 cttccacaca aatgctctta gatggacctg taaaaggcgg taatttgcgt gctgctcgtt
     8401 cagctgctga caatactatc ccacattcta ctggtgctgc gaaagcaatc ggcttagtta
     8461 tccctgagct acaaggaaaa ttacaaggac acgcacaacg tgttccagtc gttgatggtt
     8521 cattaactga attggtttca gtattgaaaa caaaagtaac tgctgatcaa gtaaacgaag
     8581 caatcaaaaa acacacagtt gacaatcctt cattcgggta tgatgaccgt caaatcgttt
     8641 ctagcgatgt cattggaaca actcaaggtt cgatctttga cccaacgcaa acagaagtaa
     8701 caactgctgg tgatttccaa cttgttaaaa ctgttgcatg gtatgacaat gagtatggat
     8761 ttacttgcca aatgattcgc ttactagaaa aattcgctaa cttgtaattt gatagatcaa
     8821 tcgataaaag aagcctttga ctggatccag tcaaaggctt ctttcttttc atcccctact
     8881 tttaaactcg ttcaatgaac atattggctt aaaaattgtt ggactacttg ctgatatttt
     8941 tcaggatctg tcttgtatgc ttccgcatgc tcggctcctt ttacaacata cttttctttt
     9001 ggtcccttcg ttgcacgata gaccttatct aacatttcaa atggtacaaa tgtatcttcg
     9061 tctccatgta taaagagcat tgggcgctgg tttttcttca attgttccac tgcatttgct
     9121 tcaccgaaaa agtagccagc acggactttt gtcactagac tcgttacttg cataagcgga
     9181 aatgctggaa gactaaacat atcgtctagc tgataagcaa gctcatcatg aacggaagaa
     9241 taaccgcaat cttcaatgat tgctttcaca ttatctggta gtttttctcc gctagtcatc
     9301 ataactgttg ctgctcccat gctgacaccg tatagaacga tttcttgcga ttttccattg
     9361 ttttctagca ctttgtttat ccactgtaca tagtctttac gctctggcca tccaaatcca
     9421 atgtaatccc cttcgctttt tccatgtcct cttgcatcag gaacaaggac attgtaaccc
     9481 atatcatgaa acatctttgc gtaattggtc atcgtttcag cgtttcccat atagccatga
     9541 gccacaagag ctgttttttc acttttcgtt tcagcaggaa gataaatcgc tgataatttc
     9601 agaccgtctt ttgaagtgat ggtccaatcg cttcggtttt cttttgaact aaaccattta
     9661 tgtgcatccg tttctttgga tgtctgtgtt gtaccaggtg tatcttcgtc taaaaagtct
     9721 ttctcagaag gcacaacagc ataattataa aaataattcc ctgcaaacac taacgcaaca
     9781 atcagagcta aagcgacgcc taaaaggatt ttcatccaaa gtttcataaa tcatccatct
     9841 catttctctt ttttcatata accttgaatt agtataatca aagaatctct actatgcaat
     9901 gttcattttt ataac
//
LOCUS       DAYYJC010000094         9729 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000094 DAYYJC010000000
VERSION     DAYYJC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.096"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..2349
                     /locus_tag="ACHA9N_002271"
     CDS             22..2349
                     /locus_tag="ACHA9N_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFM9085706.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2346..3260
                     /locus_tag="ACHA9N_002272"
                     /pseudo
     CDS             2346..3260
                     /locus_tag="ACHA9N_002272"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3333..3848
                     /locus_tag="ACHA9N_002273"
     CDS             3333..3848
                     /locus_tag="ACHA9N_002273"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFM9085707.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3845..4291
                     /locus_tag="ACHA9N_002274"
     CDS             3845..4291
                     /locus_tag="ACHA9N_002274"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9085708.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4441..5256
                     /locus_tag="ACHA9N_002275"
     CDS             4441..5256
                     /locus_tag="ACHA9N_002275"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9085709.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5462..6634
                     /locus_tag="ACHA9N_002276"
     CDS             5462..6634
                     /locus_tag="ACHA9N_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9085710.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6796..7503)
                     /locus_tag="ACHA9N_002277"
     CDS             complement(6796..7503)
                     /locus_tag="ACHA9N_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085711.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            7873..8295
                     /locus_tag="ACHA9N_002278"
     CDS             7873..8295
                     /locus_tag="ACHA9N_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085712.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8461..9654)
                     /locus_tag="ACHA9N_002279"
     CDS             complement(8461..9654)
                     /locus_tag="ACHA9N_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9085713.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT         3329 a         1867 c         1869 g         2664 t
ORIGIN      
        1 gattttttat tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt
       61 atatgtcaaa aacattttgt ttctagactc aaagctgtat ctaaaagaaa atacttacat
      121 cccattatga tttcgatcat cttgattttt actttcttag ggagcttatc aagtatttat
      181 gtaaacgttt atacagaaac gtcaaatttt gctaaccaaa cgacagagga gacaaatagt
      241 ccaccctatt ctgaaataca gtccatagaa agcaccaatc agtctagcaa caacgagaca
      301 acctcaacca catcctcgac tcaactgatt gatccgacag aaaaagaaat cacagcagca
      361 aaagcagcag gggagaaagc ttattcagaa acaggtagaa gccagacatt tagtactgtt
      421 gcggccactg cgaatttcaa cggacatacc ataccagatg gatatacagt gatatatgcc
      481 aaagatggag tcattgttgc ggttaacgga tcgatgagtg cggataaaac tgcgttacaa
      541 ggcgtaacca aagcaatcgc ttttattgat aaccgtccag atatccataa tctgtttatt
      601 ggctccacag taaatggtac tactatcaca ttcccaaaaa gtatctatat gggggtggct
      661 accgctggaa atacgatcga ttcagttaca cccaatgcgg ataaaagtta ttcttttaca
      721 ccctcaacat ggggagggct tcctgccgga ggaggtatga gtactttttc aatcggtata
      781 cagttagcaa atgatattcc attcacgggg tactcgatca aaaatgggca gatcgttgat
      841 ggtaagggaa atgtttcaaa ttttccaacc tatcaagcag caacggttca ggtcaatgcg
      901 aatcggacag gaactttttc ttatacagtc aatggtggag cagcaaaaat cggaacgtac
      961 gggactacat tatctggaat ccatgtgggt gacaaagtgg ttatcactcc aacagcagta
     1021 acgggctatt cttttgcaca aacaaacggt tctttcacag ccctagaaag taacgtcgat
     1081 acggtgacct atacaggcaa acaaacacca atcactatta agtatcaaga ctattttgga
     1141 cagatgattg ctccttctaa aataatgaat cttacttacg gaagtgctgc acaagacctg
     1201 acgactaata cgcctactat caacggatat acctttacag ctgtttcagc gatagaaaca
     1261 aagaaccaat cagcaatgag tgttttagct agtttagata caaatggaaa tgtaatcgta
     1321 aaggacgcaa atggaaaaca gatttctgaa gtcatcctat attataagac aaatgcaaca
     1381 gtatcaatca atgcaaatgg cagtaagtac tatgatggtc tttctgttgt accgatcgtt
     1441 aaatatgcct ttaatgataa aacagaatca acatcccttt taaacaatga tctcagtatt
     1501 gatcaaatca catggaatcc aactgatttc aaagcgatga atgaaaaggg agttgaagta
     1561 actgcgccta ctgaaattgg aacctatacg tcttggcaat tgacaaaaag cggactagct
     1621 aaactagctg ccagaacaaa ctatttattt actatcgtgc agacatctga tgtctatacg
     1681 attaagcaaa tcagcggtgc ggtagagctc agtggcagta agacatatga tggaaaagca
     1741 ggtgtcccgt caatccatgt caagctagca gagggtgtca ctagttcgct tactccagta
     1801 caagtggcac ttagcagtat ggattacaca gcagatgcac aatcagcaaa aaatatggtt
     1861 aatgctggca gctatacaat taaactaacg aatagcggaa tagacaaagt caaagcagca
     1921 aatcccaagt tgtcatttac tgatatagcc aacacatcag gaacttatac catctataaa
     1981 gctaacgcag tgattactgt cgatgacgct gcctttaatt atgatgccca aagtcactcg
     2041 atttcggttg gaaatctcca cgttaccggt gttgtcttag gcgaatcatt ggattatacc
     2101 ctaacagtta acagccggac agatattgga tcacaaatag taacgattag tttaggcgaa
     2161 ggaagtgtaa acaataatta tacgattatc gcaacagata ccgctaagtt gacgatcaat
     2221 ccgactctct tgcttccttc gacaggtggg ataggtctac tgccctttgt ctttttagga
     2281 ctgatcttta ttttttctgg cttcttttat tttatccatc gtaagaaaaa agcaggtgaa
     2341 cagcgatgaa aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc ttttttatcc
     2401 tactggtctt ttttataggt tctccgactg tgtcagctag agaatcacct attacattta
     2461 ctttgcataa tcgaatgttc gaaagcgata agatgccaag tatcagtcaa aacgacggaa
     2521 taaatatccc ggataacttt caaggagtaa aagggttgaa cggcgttact ttcgctatat
     2581 atgatgtctc ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac
     2641 aaagaaaact agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa
     2701 cagtagacga ggaagagggc attgcttctt ttagtgcatc ggacaaagat gagcaaggtc
     2761 gtgacgctgt ttaccgattt acggaaataa agacttcaga ttaagtaaaa gaaaaatcag
     2821 caccatttgt cgttgtgctt cctgtgacag attcatctaa tcataaattg acgaccatcc
     2881 acttgtatcc aaagagcgaa caaaaaatac ctgctgcact cacattaaca aaaacagtag
     2941 agaataagca gacagatttt gcagatgggg ataaagtccc ttatctcatc accgtaacaa
     3001 ttcctgagaa catcaacgag ataggaacct atacgatcaa agatacagct gatcctcagt
     3061 tatggctaga accagaaacg atcgattttt tgattggtgg agacaagatt catacatttc
     3121 acacacaaaa aataaaacat ggttttgtct tatcagattc tggaaaagat cttagttcgt
     3181 ttgctggaaa aaaattaacg atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa
     3241 cgacatttga taacgatgta cgtttgacga ctgacaatca aacagtagaa acacatcttg
     3301 ccatccaaac aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa caaaaactag
     3361 cagatgctca atttctagtc aaaaaaaatg accaatactt agcgcaagaa aacgggataa
     3421 atcattgggt agccaaagat gattcaagag caacgatttt tacttccagt aaagatggaa
     3481 cattttctgt ccatggattg tcttttggaa gttacgaact agtggagatt aaaccgccta
     3541 aaggatatat attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc agttatgatc
     3601 cgcttcatcc tataccattg gaaatcatca acgaaccaaa agcaaatccg acgaataatg
     3661 gaaaaacgcc agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct
     3721 ttccaaaaac aagcgaagag atgcttggag gtttctcaat tttagggtta ttgctagtcc
     3781 tttcaacagg gacagcttgg ttttataaaa aacaacaaaa aggaaacaat aggagggaaa
     3841 taaaatgaaa aacatcaaaa caaaaatatg gacgttttta ggaactgcta tcatgctgct
     3901 tcctttcgtt ctcggcctag gaactgcaga ggtttctgca gccgtatcac ctacaccaga
     3961 aaatgttacg gttaatttgc ataaattgaa attcacttca gcaccagaaa atcaaataaa
     4021 caacggaacg gaattgacat ttccgaatag cgaaccgttg aacggcgttg aattcaacgt
     4081 atatgacatc actgctacgt actatccaag taaagacaca gcagttccag cagacgctac
     4141 acccttcgca agtgtaacga cttctggtga aggattagct aacctgactc ttccaggtaa
     4201 atctgatggg aaagatgctg tatacgtctt tgtagaaaca ccaaagcccg gtgtcgaaac
     4261 gtcgccaaat atcgtgttaa gcttacccta gaccctgaca aacctttaga cacgattcac
     4321 ctatatgcta aaaatgtcat caaagaaagc aatatcaaaa tccagaaagc tgggaacttt
     4381 tcaggggtag ataagttagc aggggcgcaa ttccagatca aaaattcaga gaatcaattc
     4441 gtgaccggtt ttgatgaaaa cactggtgtc gctctctatg atcctgctga gtcagcgttg
     4501 acattcacaa ctgatactga aggatccatc agtgtcaatg gattaataga cggcaattat
     4561 actttagttg aaacaaaagc accagatggt tatcaattaa ccaatccaca aaaagaaact
     4621 acttttatag taaaaaacgg aaaattgcaa caaacaaccg gtatcatcca tggcgcagat
     4681 gaagccaatc ttgggaatcc aaacggtgta gtcaatcggg aaaatacaac tacggataac
     4741 acgattactg tagaaaatgt ccaaaactac ggtgatttcc acttcatcaa acaagatgtg
     4801 aacacgcaag aaaaattagc cgatgcagaa ttttctattc taagagcaaa agatccaact
     4861 gttaaattaa tgaaaaaaat agcaaacctt gcctcaggag aatacccata tgcttggtca
     4921 gatgaagtca caggttctga ctgggaagaa gtgaaattga tttctgattc agaagggaaa
     4981 ttttcaatcg aagggctgaa acaaggggca tactgccttc aagaaacaaa agcaccagaa
     5041 ggctacgcat tacctaagaa tgaagctgcg tacactgaat ttacagtaga tgatgatcca
     5101 acaacttctg atgaaacaac gatcgataac caacctaaag gaatcttgcc ttcaactggt
     5161 ggtatgggga tcatcgtatt catcttggta ggtactgctt tagttggagg agcagtgatc
     5221 tatttcaaaa aacgccatca agaaacagaa gcttaaacta ttggaaacaa aaatcatacg
     5281 ataaataaac gatagtgatt caaatgggga gaaaggttgt gacaagtggt cggtctcttg
     5341 gtattcgctc aacaaacggc aaaataggag gaactatttt gccgtttgct tcgctaatac
     5401 tcaggtcaag gcgctgattt cacaacctct tcatatacat attagaaaat aggtggacat
     5461 gatgaataaa agcagaataa gaatactgga catcttcatg gcaatcattt tagtcgttgg
     5521 gatcgggatc ttttcttatc cttttgtaga agattcactg aatgattttc ttgcccagca
     5581 aatgatcatc cattatcaaa agcaagcatc gaataagaat tcagaggaaa taaaaaaaca
     5641 gcaggaaaaa atgacaaaga aaaaccaaca gttggcagag caaaacgtat caccagggat
     5701 aggcagtttc aaccaagcag ttgatgcaaa agcattaaaa gatttaccta gtaatgcctt
     5761 ttttatggaa catatgcttg gagtcatcga aatcccaaaa atcaatgtca gcttacctat
     5821 attcgatcag acaacggaaa tatttttgca aaaaggaacg tcattattgg aaggaagttc
     5881 ttatccgact ggcgggaaaa acacccatgc cgttctttct gggcatcgtg gactcccaga
     5941 agcaaaatta tttacagatt tgcctaagtt gaaaaaagga gaccagtttt tcatccagat
     6001 caatggaaag acattggcgt atcaagtaga aaaaatccaa gtggtcttac ctgacgaagt
     6061 agattctttg ggtatccaaa aaggcagaga tctcgtgact ttgcttacct gcactcctta
     6121 tatggtcaat acacatcgac tgcttgtgac ggggcatcgt attccttatc aagcaaaaga
     6181 ggcaaaaaaa gcgatccaag gaattgatca atggaaaaaa tggaaatttt ttatttggtt
     6241 tatcggtata ctacttggat caatcggctt agtttggcta ctcatcgctt atttagattc
     6301 tttggccatc gctaagcgaa actatccttt atctttttat gtaaagaaca caaacggacg
     6361 accaatcgaa ggaatggtct tttctgtaaa aacgctcaac ggcaaacact atattactag
     6421 agaaaaagtt ccatttgtga aagcttctga tgaatacgga ctagtgagat tttctgattt
     6481 aaaaggcagg aactatcgct tgcaacatga ggagctcctg cttaaaatcc atgtaaaaca
     6541 taaacattcg aaacagtttt caatgaaatt gaaaaaagga agatataaac tgcgaaaaga
     6601 aaaagaggtg tactatttga ttgagaaaga atgaatgaag atgtccaaaa aagcagaggg
     6661 aacagactaa atgaaaaaag agagaagctg acgaaatgga cgtgtaaact gctatttttg
     6721 gtttactatc tggaacgcca gcttctattt ttttatgttt cagaagaatt ttattattat
     6781 agtataactt ttgaattagt ttccataaca tttggagcaa ggggttaaac cacgagcttg
     6841 agcttcgctt aaagtggcag gggtatacgt accatttcca catttatgtg tatggtattt
     6901 tgaaccggtc ggtgtgacat agacagtttg ttcattattt tgctcaggag ctgcggcttg
     6961 tgcctgctga gaagcctgtt cagctgctgc ttgctgtcgc gcctgttctg cttgttgctg
     7021 tgcgattgct tcttgcctcg cctgttctgc ttgttgctgt gcgattgctt cttgttgcgc
     7081 ttgttctgct tgttgtcgtg caatttcttc ttgctttttc cgttcttctt ctgcttcttt
     7141 tgctttgatt gtggaatcaa ttgcagcaag acggcttgtc aactcttgtt tgttttcagg
     7201 tagcgcttgg atagctgctg aagcagttgc atagttttct tttgtaggat ttgcttcagc
     7261 ttgtgacaaa gcagattccg cttctgtcac ttttttttct agtgcagctt tttccgcttt
     7321 ttgtttctct tcttcttttt tcttttcaat ttgttctttc gatgaatctg ccgtactttc
     7381 ggagatcgtg gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt
     7441 agtagcacca cacccactca acaaacttcc tgcgaccaaa aacaacgcaa ctaatttttt
     7501 cactaaattt cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt
     7561 ttacttactt gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc
     7621 cgtgttgata cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata
     7681 tgtacgttat tttattatta gattattgac atttatataa taaacgatta aatgattcat
     7741 atcaatagaa tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt
     7801 gtgacaatat gatatttgta tccgtttttt tcttgtatgt agtatagtaa agtagataca
     7861 ggaggagaaa atatgcagac ttttttaaca aaagtttctc aaaagaaaat taaaatgatt
     7921 catttactgc ttgaaacaga acgttggtgt tcaatagaag aactacaaaa cgaattgaag
     7981 gtatcaagta agtctatctt gaattatcta acagatttag aagaattgtt ccaacaatat
     8041 cctgataagg tcattttgaa gaatgagaat aatcggcgct tttctatgga aaagcaggag
     8101 aacttcccaa tctacattat ttatcttcat ttctacagaa aatcatataa ttatcatttg
     8161 attgaattca tgtatcaaca tcctgagaaa aatttggaag attatgcaga cgctcaatat
     8221 acgagtgtat ccacagtttt tcgttatgct aagttattag taaagtactt tgaaatgacg
     8281 gacgtcaaag aatagtcgcc gccagataga aggaaattag cagttgaaaa agctgccctg
     8341 catcaagtaa tacgaccagc caaacggaaa cagtgtctca tgtttttgat ttggctggtc
     8401 ttattaccgc agatgcaagc tttttccaca cctctttctt gtcatttaca gactcaacta
     8461 tcaatgctgc aagctttccc ttaatgctgt aacaccagta agagttgtca agaccagcag
     8521 actgactagg taaataccgc tcaaaaacaa gattacggca gttaaactat aatgatccat
     8581 caagaaaccg atgacgatag aagaaaaacc accaacagct cgtccaaggt tcataacgaa
     8641 attattcgcc gtcgcgcgga tttctgttgg atacaaacta ctaatcactg cgccatatcc
     8701 accatacatg ccattgataa agtagccgac tatcacagcg gctgctacca aactccactg
     8761 gctatgggct aacacaagta gatagacaga taaggatgaa caaattaaaa acaaagaaaa
     8821 ggctagttta ggccccaatt tatccatgat tatgccaaaa gtcatcatcc ccaaagacat
     8881 gcctaaaata gtactgacca tccacaatga agacccagaa accgaaagcc ctaatcgatt
     8941 ttgcataatt gaaggtagcc aattcattaa cccaaaatag ccggcaattt ggacagttac
     9001 catgatacta aggccacatg tttgccaagc aagatgtttt gattcaaaca gtctgctaag
     9061 tttggttttc ttttcggcag attgtacaga tttgaattcc tttggttcag tcaagccgcg
     9121 acgaatgaac aagaccatca acaccggcag tacacctacg acatacaaca tcttccaacc
     9181 aaacgttgga atgatcacag cagctaaaat cgcagccaaa gccgcaccga cctgcgcacc
     9241 aataccagca accgaagaag ctcgtccaat ttgttttttt gaaaaacttt cggaaatcaa
     9301 ggacatacac gccccatatt ctcctcccgc tcctacacct gcgataaagc gaaagagata
     9361 aaccaagtag atgtttgaag caaaatacat caataaagaa gcaatcgaaa acagaaggac
     9421 cgtttgagaa aatactttca cacgtccata tttatcagct atgatgccaa aaaaaatacc
     9481 tccaatgagc atccccaaat tagtgatcgt tgaaatgaaa cccgcttgcg taccactaat
     9541 atgaaaagaa gtaatcattg aagataatga aaaagctaaa aacataatat ccatattctc
     9601 taaagcaatt cccgccgatg tcgatactaa tacctttcgc tgatacgttg tcatttttat
     9661 accccttttc ctaaaatttc cctaaattgt taaaagtata tccaaaaaag aataaaaaaa
     9721 caagacttt
//
LOCUS       DAYYJC010000095         9350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000095 DAYYJC010000000
VERSION     DAYYJC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.097"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..749)
                     /locus_tag="ACHA9N_002280"
     CDS             complement(81..749)
                     /locus_tag="ACHA9N_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9085714.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            883..1170
                     /locus_tag="ACHA9N_002281"
     CDS             883..1170
                     /locus_tag="ACHA9N_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085715.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1586..3124
                     /locus_tag="ACHA9N_002282"
     CDS             1586..3124
                     /locus_tag="ACHA9N_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9085716.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3121..4086
                     /locus_tag="ACHA9N_002283"
     CDS             3121..4086
                     /locus_tag="ACHA9N_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085717.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4073..5395
                     /locus_tag="ACHA9N_002284"
     CDS             4073..5395
                     /locus_tag="ACHA9N_002284"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9085718.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5458..5562
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5636..6379
                     /locus_tag="ACHA9N_002285"
     CDS             5636..6379
                     /locus_tag="ACHA9N_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9085719.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6394..7386
                     /locus_tag="ACHA9N_002286"
     CDS             6394..7386
                     /locus_tag="ACHA9N_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9085720.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7398..8135
                     /locus_tag="ACHA9N_002287"
     CDS             7398..8135
                     /locus_tag="ACHA9N_002287"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085721.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8125..8847
                     /locus_tag="ACHA9N_002288"
     CDS             8125..8847
                     /locus_tag="ACHA9N_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9085722.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8931..>9350)
                     /locus_tag="ACHA9N_002289"
     CDS             complement(8931..>9350)
                     /locus_tag="ACHA9N_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9085723.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3065 a         1607 c         1972 g         2706 t
ORIGIN      
        1 agaagatttt actgaataat atcattttag tttacataat gatatttctg taaattaaat
       61 aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag tggttcaaat
      121 gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc cagatacttc
      181 actttattca atgtataaga tagaatagaa cagctaagta atgtatacag aaaatgtgtg
      241 agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa aagacttttg
      301 ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt tccgcctgtg
      361 cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa
      421 ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa
      481 aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga
      541 aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga aggagcgaat
      601 ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc cagcttagat
      661 ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat gattccgcac
      721 aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga aaattttcag
      781 caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa aaaaaattat
      841 ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc agcagactta
      901 ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt tttggctcaa
      961 gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca atttatcttt
     1021 ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt tttgatgaag
     1081 aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct ttatggagtg
     1141 aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat
     1201 gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag tataatattc
     1261 gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct
     1321 ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt
     1381 cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt
     1441 tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt ttttttgttc
     1501 gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat gtttatactt
     1561 aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa gtgaaagaaa
     1621 aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt ctttgctgat
     1681 tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa aaatggcaga
     1741 agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct tattaggttt
     1801 acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg ttatttacgc
     1861 tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc cgtctattga
     1921 agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag tagagcttcc
     1981 catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa tcatcggtac
     2041 ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt tgttaggtat
     2101 tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct tgcttgcaat
     2161 tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt ccatcattac
     2221 tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg ccttgtttaa
     2281 aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat
     2341 gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac cgaattttgg
     2401 caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct atcctgagtt
     2461 cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt
     2521 gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat tcgatatgac
     2581 attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga gatcttttgc
     2641 gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc agatcaaagc
     2701 gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc aagaagcata
     2761 tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa
     2821 taatggagat gtcaatattg tggatgccta ttccacagat agccagctca aggagtacga
     2881 tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg caccgctgat
     2941 gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata aattaagcgg
     3001 aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa
     3061 gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg aggaagatca
     3121 gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc
     3181 gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg tccttccggt
     3241 agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc agcaggagac
     3301 atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg gtggaatatg
     3361 ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca aaatattgaa
     3421 gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt agatgaactt
     3481 cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag agagttgtct
     3541 ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc agatgtgatt
     3601 ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt acaagaattg
     3661 gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca caatatggaa
     3721 gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat tatccaatgt
     3781 gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca tttctttgat
     3841 gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg atatttctta
     3901 aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat gaaagaagta
     3961 tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag aagtattggg
     4021 tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac
     4081 atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt atggattgaa
     4141 agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga cttgtcctaa
     4201 ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa
     4261 acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag aacttcaaaa
     4321 attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat tagcagaagc
     4381 tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta ttgaagtcaa
     4441 cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac ctacgatctt
     4501 accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat tgcctgtact
     4561 gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag ctacgattgc
     4621 caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat taaaagaatt
     4681 tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca ttcagtttgc
     4741 ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt tagtcggaaa
     4801 agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc cgaatacaga
     4861 atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg cagtcaatga
     4921 ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt caaggaaaac
     4981 cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc ggataacgga
     5041 tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg ccagtcctaa
     5101 acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag tggtagagat
     5161 ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt
     5221 gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc aagcagacga
     5281 attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag agttggatag
     5341 ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga aataatcagc
     5401 atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa aaatgaatac
     5461 ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga cccattgaac
     5521 ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg gttactatgt
     5581 cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta acaaaatgaa
     5641 gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc agattgttgt
     5701 catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat tttctgcatt
     5761 agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg aagcagtgct
     5821 tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg attattggaa
     5881 gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc caatcatggt
     5941 gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag tgatccttgt
     6001 tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga ggaaagtatc
     6061 aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga tttatcgaat
     6121 atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg ctgctactta
     6181 ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct tagggtatta
     6241 catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga tcatttgtgt
     6301 cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg
     6361 tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct tttattaccg
     6421 ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa acaagaaata
     6481 aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa tcatactggc
     6541 atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt ccaaatcatc
     6601 gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc acaatttggt
     6661 gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat accagtcaaa
     6721 gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc agacagtaac
     6781 atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa
     6841 gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa actgacaatg
     6901 gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat ccaatggtac
     6961 tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa ctatatccca
     7021 ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac gaatgatcaa
     7081 ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa aaaaggctat
     7141 caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa
     7201 aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta tacggatgat
     7261 cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc ttttatgcag
     7321 gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa tcagtttata
     7381 aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc attgaaaacc
     7441 tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca tcgattgtcg
     7501 cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc cagcctatga
     7561 cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca gcatttagtt
     7621 atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac gttacgttat
     7681 atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat ctggatatct
     7741 ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg atgaagcagc
     7801 gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt gatgaaccat
     7861 ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg aagctgccta
     7921 aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca atttatttat
     7981 cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag attcaagtgc
     8041 ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag caaacgattt
     8101 atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt agatgggagt
     8161 cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa ttgtgattcg
     8221 ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc cttaagttac
     8281 ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc ttttttgaaa
     8341 aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt accgatgact
     8401 acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga gaaacctgtt
     8461 gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc ttaccctaat
     8521 cactatttga tccattggta ttcttctttg gactatcaac aagactatat tgatttaggc
     8581 tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca actagctaaa
     8641 gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc agagtgggct
     8701 ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca gttaaagaca
     8761 atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta tcaaaacttg
     8821 gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt gtcgcacctc
     8881 tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc ttagtaccaa
     8941 ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga agtaacatat
     9001 tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta agaagaatct
     9061 aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct tgattctttt
     9121 tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt ttctgttaca
     9181 gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg aaccgtttct
     9241 tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg aggttcagca
     9301 gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DAYYJC010000096         9328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000096 DAYYJC010000000
VERSION     DAYYJC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.098"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..364)
                     /locus_tag="ACHA9N_002290"
     CDS             complement(<1..364)
                     /locus_tag="ACHA9N_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9085724.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKK"
     gene            complement(354..2528)
                     /locus_tag="ACHA9N_002291"
     CDS             complement(354..2528)
                     /locus_tag="ACHA9N_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFM9085725.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2544..4886)
                     /locus_tag="ACHA9N_002292"
     CDS             complement(2544..4886)
                     /locus_tag="ACHA9N_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9085726.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4908..7451)
                     /locus_tag="ACHA9N_002293"
     CDS             complement(4908..7451)
                     /locus_tag="ACHA9N_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085727.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7461..7859)
                     /locus_tag="ACHA9N_002294"
     CDS             complement(7461..7859)
                     /locus_tag="ACHA9N_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9085728.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7940..8224)
                     /locus_tag="ACHA9N_002295"
     CDS             complement(7940..8224)
                     /locus_tag="ACHA9N_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085729.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8248..9246)
                     /locus_tag="ACHA9N_002296"
     CDS             complement(8248..9246)
                     /locus_tag="ACHA9N_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9085730.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2651 a         1999 c         1461 g         3217 t
ORIGIN      
        1 tttttttgac gtttctatca tgaatcacga gaaaacttgg gacactttta acatggtaac
       61 gttgtaaaaa agtttgaagt tcttcttgtc ttggttcttt tcttgtttca attcgatata
      121 cattcctaga gaattttttg tcgtatcttt ccaagaaaga ttcaacttct tggcattttt
      181 tacaatcatt tcttgctaga tacacaaaaa atgattgatt cgttttttga agttctagta
      241 cttcttctaa attactattg attagttttc catttgattc ttcgttagtt tttatcagtt
      301 taaaacaacc aaataagagg aaactaacaa taagtatcct aacaataact ttcttatttc
      361 gcatattgtt tctccaaatt tggtttccga atatagacga ctcgcggtgg ttcctggacg
      421 tcttcagctg ttcgatacac cacaaattcg cctgttctca atgctcgaat gcgattcgga
      481 ttgaatttaa agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca atcaaccttt
      541 cccatatcag aataacctgc ttcttgtgtc gtcatttccg ttaattcaat gtcttcttct
      601 gtccccatta aatctgccca ataatcaatt tctgtcgggt catttgtctt cccaataaag
      661 aacgtattca cattcccaat caactgtttt cgaaggacag gatcaattct atcaatgtcc
      721 gcaagcgttt gaggggcata cgccgcatgc accccagcgc ctcgtccctt attgactaag
      781 tctataaggt tatcatttag ataagaagct ggttcgtcaa atattactag gactttcgag
      841 cgattggctg tttctcctgc ttgtttatac acttctgacg caaagaaatt aatatctccc
      901 acaagcattt gagccaatgt ccgaataaac tcactataaa tcaatccgtt taacgagata
      961 taaacaatct ttccttcttt aacaacttct tgaatatcta attctctttt tcctccagaa
     1021 gtatcaaaga gttcaccaag ctcactatcc aataacatat tgatattggt ctgtaaattc
     1081 tcagaagtcg catacaaata aaacgggttc ttcttatgtt cgtaacgaac aaacaaccgt
     1141 ttaaacaacg tttgctcgat ttctgtcatg agagaaatgt tttcttttaa taatagataa
     1201 tacgtttcta agtcaacggt tttacgatca tgttcaacga ttcgctcttc acttaactgt
     1261 tccacatcgg caaaatcacg ttctgttttg tccatcgctt cattcttttt cgtttttttc
     1321 ttcgtggttt tcttcttttc tacgaccact ttttctttaa ataaatctgg atttttcaac
     1381 tgaatacgat ccgcaaacaa atgtaagacg tttcgtggca ataaataatg ttgtaaccaa
     1441 actagatttc tttcaatttt atggctcttt tggaactcgt ctaaaagctg aatcgttacc
     1501 tgtaataacg ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc tagattggtt
     1561 aatttatctc gaatggcaat atcattgcca ttttttacag gattataacg caaatcgcca
     1621 ccgtccacaa agacttcaat cgggcgattg tatgcctctg ccacttcttt taacgcttgt
     1681 aaagtctctc ctgctccttt cccatcgact aagataacgg ggatacgaaa cttacacgca
     1741 tgttctgcta gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc tggaataatc
     1801 acatgttggt tcaattcact aaagttcatc acataaggga gcccatactc atctgttcct
     1861 aatagaatca ctttggctaa tgaagccagt tgtttattcc gcaaagcaac gtcttttatg
     1921 acaattgcta cctggtacgc ttgctgatac tctgctgtat atttttggga ctcctgaaat
     1981 ttattcttcc gacgttcctt gtattcttta ctatttagca aagctgtttg ccgttctttc
     2041 tttgaaacaa cttttctttt ttcaataaaa gacgcatacg caatcaaaca acttcctgca
     2101 cctgtcccac ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa cgcatgagaa
     2161 aacaagttta atggaaataa taagtaacac gacaaatacc ctctaggaag aacaaaaata
     2221 aaacaaaata gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag agagcttctt
     2281 cgttttaaga agctctcata ctcaaagtca taaatatgtg cacgtgtctc ttttttatct
     2341 atcataaaga caatcgctaa aggaatactg cctacaataa aaaatggata tagacaaaca
     2401 cctaataaag tgacgataaa ataaagttgt tgaaaccgac tttttggcgt ttctttttct
     2461 tgttttcttt tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg ttttggtgca
     2521 ttcatcatac tatcgatttc ctcctatcgt tttcgttgtt tcatttctct tcgataaatc
     2581 ggttgttttt ctcgttttcg tttcattttt gcttgagttg ctcgaaactc actccgtgtc
     2641 ttcggtgctt gttttggtga ttttgtttgt ttcatggttg agtatgcttt agatacggtt
     2701 gaaggcgctt tggtttgtgc tgttactcga ttgactgctg acatatgttc ccgaaccccc
     2761 tctggacgtc gaacagattt cggtgtggta gaacctgttg ctcgtggtac attccctttt
     2821 cttagtggtt tttgtaccgc atgtttttgt cctgcaactg tccgggtttt catcgaactt
     2881 gtttttgtta cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc ctgacgacgt
     2941 tttcgatcgt tcaattctgt tctaggcgta tgcttctggc cataagactt gtgtttttgc
     3001 tttggattcg ctaatgacac caccattgca gaaccctgat tgcgatacgg actacgagac
     3061 gcaccttttc tacttctttc tcttagacta ggattgcctt gcaattctcg ttgtaataaa
     3121 tcggctgttt ttcttgcctg tttctccatt gctgttcgtt gcatacgttc ttgactttgc
     3181 tcttctgctt gtttcgcttg atcatgcaag tctcttgctt tttgtgccat acgttccata
     3241 cgatttggtg ctttatgacg aggttgttgt gcggattcac gccgaaaagc acttcgtgtg
     3301 gcttgtttcg ccgtttgtgg catacgaacg tctgagccac ccccacgttt tcgttgacgt
     3361 tcttgtctct tccgtgcgtt ttctttttct ttttgtttct gtgcttttct cgcttctttg
     3421 cgttctttca accgttgttt ggcttccctt ctcaattttg cttctgcccc aaatggattt
     3481 cccgcaaaag ctaacgctcc tcgaacaccc attcttccag taaatagact gcctaaaaag
     3541 cctcggaaca aataaattcc caacggcgca aacaaataaa caatgatcaa gagaattttt
     3601 tcccatggat cgtcttgtgt actacttaat tggaacccgt aactaagaaa agaagaaaaa
     3661 aggatacatg caaaacctgt cattcctttt aaaaacgtca aaataccaaa aactttggca
     3721 tagttaccaa acacattcat tgtgacaaga aacaatccta aaaaaagtaa gaacggcaac
     3781 atcggtacta acaatagttg catagttcca ataatcaatc gaaacatact taagacaaaa
     3841 taaacaacgg tttgaatcag attcacgaca aaataaaaca aggcaaatgc tgtatttttc
     3901 caactattgt aatattgtat gttcttgttt ttcagtgtat ctgcttcata ctctgtgact
     3961 ttgtcatgaa ttttctcccc tatttttgta cctaaatcat tgtccaataa aagattgatt
     4021 cgatcatatt cttttccagc atatttgact ttcttctcac gaattgtgtc tacattggtt
     4081 gtgccataat ttaacaacag atatggttca taaacattac tataaaagac ccgagccgcc
     4141 atcaatttgc ctgccgtttc cactttttta tccgctgtta cctcttctgt ttcttttgtt
     4201 tcataatttg tttcttcttc aggtgttaac actggattga ccttcacgaa cgccgtttcc
     4261 actgtagaat ctaaagaaaa aagaacatca aaaagattct tatttgtccc agaatctttt
     4321 aatacgctta acccagtaaa aataaggatc gtcataagaa agacacggag tgcttgtttc
     4381 attcgagcct ctccagcata tttgattgcc atcataattg ccgaaaaact gattccaata
     4441 gttcctgcaa tacttaacat attgtccgca attttttgtg ttaagttttg aatgggttct
     4501 cgaatggcgg aaataatatc taattcaaag agaaattcaa ccattgaagc attgaattct
     4561 cctaacattt ttacgccact ccaaacaaaa tttttcatca ctcctgcaac attgtacgat
     4621 agcttttttt taaggttcat tgactcatag tcattggtca tcaattcaaa cgaattcttt
     4681 ttgtaatttt tatacaattg atccacatca gtcgttactt cttttacttc tactttatcg
     4741 ttggcattct ctccttcaga acgatcggtt tcagtgacac ccgcaccatc attcatttga
     4801 tcggcagaaa cagtagcccc ttgtagacag aaaaaaagac cccctaataa aaggagcgtc
     4861 caccatttca gagatttttt tcgcaaaaaa accacctctt ttttgtttta tcgttgtttt
     4921 tcattttcta acattgcttg aattttttca tcttgatttt cagtagaaga aacagccaat
     4981 aaccaactat caaacaacac atcaatggca atcggttgat ttcttcccaa atgatcctgg
     5041 aataaacagg ttcctgtttg taattgttcc atcatttcta aattggctga attcacttcc
     5101 atatcaaaga aggacaatag tttctctcgt gcttcttttt gtttcggacg aaacgcaaat
     5161 ttgtaagaaa ttaactcttt tttatcttct tgatcgtaat ctttaaacgc ttgcacaaca
     5221 aggtaaatat ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat caaatagctt
     5281 ccttgtgcgg tatcttcata cccttttgcc tcgtcataaa taatcgcagc gtcttcttct
     5341 ttgtttgtag aaaagatata cgtaaacttc ataatgactt ccataattgc cattcctgca
     5401 agttgttcgc tatttaatcg acccgaatca atctcttttt gtgtcggaat gcgtattcct
     5461 tgtgtcccta acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg ctcattccca
     5521 atgagaatct ttcctaaacc aacgctatgc cctaaaatca atttagctag ttctttgtca
     5581 ctttttcgaa tttcttcgat aacttgactt aacgaatgct tacctggcat ggtgtaaact
     5641 acatcgatgg cgtcttggat caccgttttc ttgtggctcg ctgtttctcg attggtatct
     5701 acttctccta acattcctaa cacattttta gcggtttgaa ttgcctcttc ttttggtaaa
     5761 aacaatagag gatcaagcat ccctcgacag ctttcatctg aagataacga aataaaatta
     5821 attcgttcat acatagccac aaactctggt atgtgtccga atttttcaag tgcctttaaa
     5881 aagaactttt gggtttcatt tttcggatca atataaagaa ttttttgacc taaaaacatc
     5941 agccataaaa aaatatattt tacaaggacc gattttcctt tacctggtgg accaataatc
     6001 aacgtatttc cattggtatg ttccgcgcct tcaatacttt tctttgttaa attgggataa
     6061 taccatacta gtttactaga tagcgctaac gcttctttcg cagatttaaa cttttttgca
     6121 ataacaatcc gtcctaaagg aaatcctttc ttatttccta cttctttctt taagtccaag
     6181 ccataatctg ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa acgttgcgta
     6241 cttccaatta agcattgatt aaacaacgtt agctgatccg ctaccgatcg ataaaatttg
     6301 aaatctgtag actcaatcgc aaattcaata tctttgactc gttcttccaa ttcttctttt
     6361 gaagaagcag atacaaccag cgtgacggta gaataacaca atcgtttttc tttgcgcttc
     6421 agcttatctt ttacatccat taaccgttct gtaccaaaat ttaatacatc gtctgaatct
     6481 aaaatgccgt caacggtctc atattctttt tcttgttcat aaatccgtcc agccatttta
     6541 ttaatttttc gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg cgtatcaatt
     6601 ggaaaagata agctatcctg aagattttga ataaagctac tcccatacat ggctaaagga
     6661 gcgtccgttt gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc aatcgcaatg
     6721 tatcctgctt cattcgaaat gacgccctcg caaatttcca ttggcgtaag ctgtctttca
     6781 ggcaattttt cttctgatcg atggaaaaaa taataataaa tttgtccaat ctcttgggca
     6841 gataagcgct ctatcggctt ataattggac aattccgtat agagctcctt ttctaatgtt
     6901 tcatacgttg gtaacacact acttatagga actcgttgtc ccgtaatacg atagataaac
     6961 tttttaaaaa catgcttaag caattgaaaa tattcgatcg gatcagacac tttcatttct
     7021 gtagtgaaac gcacaaacag atacgttcga tactcgttta attgcacttc gtcttttaaa
     7081 atctttcctg cctgttcaaa gtacactttc ccaagatctg cgcattctcc ctttaccaaa
     7141 tctcgaatgg tttcttcaat gtgcgtatct aggttaaatt gacgtggaat attgataaaa
     7201 tgatagtcat attcatcatg tccaagtatg ccttcgccat cttgaagata gttcttgaaa
     7261 aaatcaatgt catttaaagg aaatacttgg gaagaaagtt gatagcctac ccacacatca
     7321 tcatttttgg tcaaaacaaa atttcggata atggcgtcta tcggaaattc taatcgagga
     7381 agcttcgttt ttttcttttt gaactgccaa atcccgataa taaagacgac caaaagaaac
     7441 aaaaacgcca tttactcgac tcactccttc catttgtgta gggaatacgt cactttttca
     7501 tggaaaaacg gcacatacat ttcatgttca aagcgttgtt tccgtttctg ataataggac
     7561 gttcgcccaa ttaaatactc gaaaaagtgt ttattgtccc atttcattga aaacaatgtc
     7621 caaacgagaa caccaatccc aaaaatcata atcaaccagg actttcgaaa cagtgtctgc
     7681 aagaaactaa tttgattcac atacgcaatc aaaaatagga tcaaaagaat gaagagaaat
     7741 accccaccta tgactaattg ttccatgcga atgccattag aaatccaggg aattttttgt
     7801 cctttaatcc cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt gtattccatg
     7861 ttcactctac ctccttttac aaaaaaagaa gcgaccagaa gttctaatct tctgatcgct
     7921 tttgcagttt gtctttttct tatgataatt gtatccaggg cacaatcatt tgcgttaccc
     7981 ccacgttctc ttcactatcc ttaataaaga taatgttctt tagccattca ccaatatcaa
     8041 cggcattcga taacaataag acgagcatta tcaaaataaa gacgccaata atggcaaacg
     8101 ctgcccgcgc aaagccttgg gtcgcataag caccaaccat tagaacaatc gcaacgattg
     8161 ccaatagcca aatgacttgt ccagatacat catcaaataa cgatttaaga tcaaaaagtt
     8221 tcatttccat tctcctctaa gttcatttta ttcatttaac gtagtaacaa aataactatc
     8281 gccacttgtt tttacttcca ttgaaaagtt tgattggaac tcgtttcctt tctcaaaata
     8341 gaaacgatac ttcccttgca aaaagtagtt tccttcttta ttttttactg catgatccag
     8401 ttgtacattg atcaattcgc cgccttctaa ccctttttcg ttagaaatca atgccaattt
     8461 ttcatcattt ttgacatata actcaaaaaa ccgtttcaca aatgcttcca gcttgatttc
     8521 ttcgctttca gtgaccgctt gcccagaaga ataaaatcga ttttcctgat agttcacaga
     8581 attggtattc ttactctcag aaagattcaa aaattgaacg ccatcaagta cttttaaaac
     8641 attgttttga taactgactg gaaaagaaag ttgactgctt ttccaatgtt ctccttctgt
     8701 aaaagtaacc tcataaaata ggcgatacat tgcttgtgtc aaatcgattt cttctagttt
     8761 aataaagcga acatctttcg ctactcgatt ggtagccgtc gttacaaagc ctagatcaga
     8821 cgcttccaat ccttgtgcta aataatgatt gacggaagcc tcccattcgt ttcgtttttc
     8881 tggcgaataa gtaaataacc tttcaataaa atgttgtccc tcgtacttca attgttccgt
     8941 agaagacaag gtttggttga cttcagttaa caatgtttct ggattgactt gttttttcac
     9001 tgctaaacgc gttaatgtat ccactcgtcc aaagtttagg aggagcacga cacccattaa
     9061 cagaaacaaa caaaaaaaag cgcctgaaaa tacactcatg gcactttttt gcgaaaattg
     9121 gatcattcga tctgacggtt ctgttttctt tttttggaac aaaaaacgtt tcttttgttt
     9181 cttttccttc tgttgtttcg gtggcttagt ttggaacgct tgtggattaa attttgcatt
     9241 actcatgaga aatgccatac cgttctaaca ataatttcgg tattttgtcc acaatcacta
     9301 cttcgtggac aaccacactt gccccctc
//
LOCUS       DAYYJC010000097         9281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000097 DAYYJC010000000
VERSION     DAYYJC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.099"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHA9N_002297"
     CDS             <1..435
                     /locus_tag="ACHA9N_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9085731.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHA9N_002298"
     tRNA            complement(759..842)
                     /locus_tag="ACHA9N_002298"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHA9N_002299"
     CDS             1370..1870
                     /locus_tag="ACHA9N_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085732.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHA9N_002300"
     CDS             complement(2060..2404)
                     /locus_tag="ACHA9N_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9085733.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHA9N_002301"
     CDS             complement(2558..3478)
                     /locus_tag="ACHA9N_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9085734.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHA9N_002302"
     CDS             3665..4156
                     /locus_tag="ACHA9N_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9085735.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHA9N_002303"
     CDS             complement(4180..6555)
                     /locus_tag="ACHA9N_002303"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9085736.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHA9N_002304"
     CDS             6881..7507
                     /locus_tag="ACHA9N_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9085737.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHA9N_002305"
     CDS             7530..8234
                     /locus_tag="ACHA9N_002305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9085738.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHA9N_002306"
     CDS             8426..9259
                     /locus_tag="ACHA9N_002306"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9085739.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DAYYJC010000098         8800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000098 DAYYJC010000000
VERSION     DAYYJC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.100"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ACHA9N_002307"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ACHA9N_002307"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9085740.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHA9N_002308"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACHA9N_002308"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9085741.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="ACHA9N_002309"
     CDS             complement(3072..4325)
                     /locus_tag="ACHA9N_002309"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9085742.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="ACHA9N_002310"
     CDS             complement(4331..5254)
                     /locus_tag="ACHA9N_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9085743.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="ACHA9N_002311"
     CDS             complement(5475..7967)
                     /locus_tag="ACHA9N_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9085744.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="ACHA9N_002312"
     CDS             complement(7974..8441)
                     /locus_tag="ACHA9N_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9085745.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8577..8650)
                     /locus_tag="ACHA9N_002313"
     tRNA            complement(8577..8650)
                     /locus_tag="ACHA9N_002313"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8614..8616),aa:Arg,seq:acg)
     gene            complement(8708..8781)
                     /locus_tag="ACHA9N_002314"
     tRNA            complement(8708..8781)
                     /locus_tag="ACHA9N_002314"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:Pro,seq:tgg)
BASE COUNT         2441 a         1867 c         1461 g         3031 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8581 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8641 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8701 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8761 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DAYYJC010000099         8777 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000099 DAYYJC010000000
VERSION     DAYYJC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.101"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..690)
                     /gene="rpoE"
                     /locus_tag="ACHA9N_002315"
     CDS             complement(70..690)
                     /gene="rpoE"
                     /locus_tag="ACHA9N_002315"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9085746.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="ACHA9N_002316"
     CDS             complement(766..1203)
                     /locus_tag="ACHA9N_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9085747.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="ACHA9N_002317"
     CDS             1366..2196
                     /locus_tag="ACHA9N_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9085748.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="ACHA9N_002318"
     CDS             2180..3547
                     /locus_tag="ACHA9N_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9085749.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="ACHA9N_002319"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="ACHA9N_002319"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9085750.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="ACHA9N_002320"
     CDS             complement(4418..4861)
                     /locus_tag="ACHA9N_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9085751.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="ACHA9N_002321"
     CDS             4995..5366
                     /locus_tag="ACHA9N_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9085752.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="ACHA9N_002322"
     CDS             complement(5535..6317)
                     /locus_tag="ACHA9N_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9085753.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..7285)
                     /locus_tag="ACHA9N_002323"
     CDS             complement(6314..7285)
                     /locus_tag="ACHA9N_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9085754.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7377..8771
                     /locus_tag="ACHA9N_002324"
     CDS             7377..8771
                     /locus_tag="ACHA9N_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9085755.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         2715 a         1794 c         1496 g         2772 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa attaataatt ttcatagaca attccctcct cacaaaagat taacttctaa
     7321 aatcaataca atcaatggta aaattgtatg gtatacaatt ttaaggagtg gagaaaatgc
     7381 ctattaattc ttatgacaat tacccgttaa cttggcgacc agacaaacaa aaattgaagc
     7441 gtccttatta tcgatcgttg attcaacaat tagaacaaga tattttaaag aataaattac
     7501 agaaaaacac tcagttaccc tcccaaagag agctagctga ttatttagat ttaaatttta
     7561 ctaccgttgg caaggcttat aaatatggaa ttgagaaagg tttgctgtat acgaatattg
     7621 gaagtggcac atttatttct cccaatgtct ttgagtccat caccatctct accaatgaag
     7681 taaccgaaca tatcattgat tttggtctgg ttagttcttt tgaagaatgt aataaattcg
     7741 ttgtcccgtt tattcaatca actagtaata acccaaatct tgttggtttg cttaattacc
     7801 aagatcccaa aggcacaaat tttcaattga ctactgcagc aaattggtta aaaactcaag
     7861 gagtcattcc gtccttagaa tctctatcta ttgtttccgg tgttcaaaat ggattggcta
     7921 tcacactttc tgcacttttc tctccaggtg atagaatagc aacagaccga tatacatacg
     7981 ctaatttcat tgaattggct cagttatatc atttagagat cgttccaatt gattttgacg
     8041 agcaaggaat gcgacctgat atattgattc aggaatgtag aaaaaagaag attcatggtg
     8101 tctttcttat gccttcttgt aataatccta tagggtttca aatctctcac aaaagacgag
     8161 agcaattagc cgaagtgatt gaaaaagaag gtctatgggt tattgaagat gatatccatt
     8221 catttttaac aacttactat caacatacga tcattcctcc tttccaaaat ttattaccta
     8281 accaaacaat ctacttagct ggaatgacaa aatttatttg ttctggattg cgagttgctt
     8341 atcttgtagt tccagtacat gtccaaagca agatagaaca agcaattttt aatataaatg
     8401 tgaaaacttc tggattagat gtggaagtca tcactcaaat cctccattca aatgaagcaa
     8461 ataggatctt agaaactaaa ttaagtctta caaaaagagc caatcagctt tttgattctc
     8521 ttttcaaagt agaaaaacca acgaatcgtg tcccttacta tcgaattctt ccagttgatc
     8581 ctgagattga ccctaagata attgagaatg actttcttgc taacggtatc cgactttatc
     8641 attccagacg ctttactaca cgtgcgcaac cagatgcatt cgttcgaatt tccctatcct
     8701 ctaataacct tgaaatttta gcttctggac taagtatcgt aaaagaaaaa ataaaaaaat
     8761 atgaattata acctatt
//
LOCUS       DAYYJC010000100         8766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000100 DAYYJC010000000
VERSION     DAYYJC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.102"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1192
                     /locus_tag="ACHA9N_002325"
     CDS             <1..1192
                     /locus_tag="ACHA9N_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9085756.1"
                     /translation="AVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHM
                     PLVTRLKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSC
                     QVSLTTVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLI
                     AVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKH
                     MNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKY
                     PFLTEAMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFRE
                     KLQNLLTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL
                     INVR"
     gene            complement(1371..1706)
                     /locus_tag="ACHA9N_002326"
     CDS             complement(1371..1706)
                     /locus_tag="ACHA9N_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9085757.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1690..1953)
                     /locus_tag="ACHA9N_002327"
     CDS             complement(1690..1953)
                     /locus_tag="ACHA9N_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9085758.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2413..3216)
                     /locus_tag="ACHA9N_002328"
     CDS             complement(2413..3216)
                     /locus_tag="ACHA9N_002328"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9085759.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3194..4318)
                     /locus_tag="ACHA9N_002329"
     CDS             complement(3194..4318)
                     /locus_tag="ACHA9N_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085760.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4334..5149)
                     /locus_tag="ACHA9N_002330"
     CDS             complement(4334..5149)
                     /locus_tag="ACHA9N_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085761.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5139..6011)
                     /locus_tag="ACHA9N_002331"
     CDS             complement(5139..6011)
                     /locus_tag="ACHA9N_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085762.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6096..7385)
                     /locus_tag="ACHA9N_002332"
     CDS             complement(6096..7385)
                     /locus_tag="ACHA9N_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9085763.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7529..8062)
                     /locus_tag="ACHA9N_002333"
     CDS             complement(7529..8062)
                     /locus_tag="ACHA9N_002333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085764.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8108..8251)
                     /locus_tag="ACHA9N_002334"
     CDS             complement(8108..8251)
                     /locus_tag="ACHA9N_002334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9085765.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2713 a         1865 c         1397 g         2791 t
ORIGIN      
        1 tgctgttctc tcgcctactc ctcgtgcttg tagaagttgt ggttctactg tggtagatgg
       61 aaacgggaaa gcaattatag tgaaaaatgg gaagaaggaa acgattgtcc gttttgaaca
      121 atacaatcat atgcctttgg ttacgcgcct aaaaaagcaa cgctatacct gtaaaaactg
      181 tcgtacccat tggacggctc aaagttattt tgtccgactc agccattcaa tcgcagatca
      241 tgttcgatat aaaattgctt ctttactgac tgaaaaagta tctttgtctt ttattgcgaa
      301 aagttgtcag gtatctttga ctaccgttac tcgtacattg aaagagttta aaagctattt
      361 accaaagcct tctaagaaga ttctccccag agtattgatg gttgatgaat ttcgttcgca
      421 cgcttccata gaagataaaa tgagctttat ttgcgcagat ggtgaaacag gaaacttaat
      481 tgctgtcttg cctacgcgta aattacctcg attaacaagc tatttcttag gctgcaccaa
      541 tccagaagaa gtagaattct tagtcacaga catgaacgcc gcttacttcc agcttaccaa
      601 acgtgttctg ccaaatgcga aagtagtgat tgatcggttt catattgtca aacacatgaa
      661 tcaggcactc aatgagttgc gtatccgtga aatgaatgag cttcgtaaag caggacagaa
      721 aagtcaggca gaaaaactga aaaagaactg gcgctttctg ctaaaaaatc gtgcaagcat
      781 caaccattat gagtacaaaa catggaaaag tttccgagca ccaaaatacc catttctcac
      841 tgaagcaatg atgattgatc gattgcttga gttttctgcg cctctaaagg aggcgtatcc
      901 cttttttcat gaattagtcg aagcctttcg agacaaagac cctgacttat ttttctcctt
      961 attggcagaa cttcccgaaa cgttagatga cggctttcgg gagaagcttc aaaaccttct
     1021 gacctatgaa gaaggtatca ccagcgcaat gatctatcct tattccaatg gaaaaattga
     1081 agcgaagaat acccacataa agacaatgaa acgagtatcc tacggattta aatcattcga
     1141 gaacatgaga attagaatct ttttgatcaa tcaattaatc aatgtaagat aacaaaaaat
     1201 ctgaaccaga atgtggttca ttctgacccg gatttgattt tgcacaactc atcagtcctt
     1261 attgacaaag agccaaaaat ctgagccaga atgaggttca ttctgaccca gatttgattt
     1321 tgtacaaccc atcagtcctt attgacaaag agcccttctt tttagtatta ttataattct
     1381 tccatgcgtt cttccagtcg ctgtaaatcg cttggactta attccccaat ttttttccat
     1441 ttgacctttt tgttgttttt gggtaattcg tttagagttc ctacgtcaat ccaagattct
     1501 ttttttagtc ctgcctgttt ccaatccctt atttcaacgt atttcagctt tattttatcg
     1561 gatttagtat gatattttga agtaattgac agaaacgaaa ttgtgtccag tccatttttt
     1621 agaatataga caggccggcg tttaccgccc tctgcagctg tgaaagcaac gtagacggtt
     1681 gctatatcat tagtattcat cttcattgaa ccattcttca attttttcgt cagtgtctag
     1741 tttttggaca ggcaattttt ccgctaatct ttgaatatta tggattcgtt tacttttttc
     1801 cgacaattca atagaaaatg ggatttttcc ttgctcaaca atttgcttat aaaaaacggt
     1861 gacggctgtt ttggggctca tgcctagttc atcaataatg ctttctgctt catttttcaa
     1921 tttttcgtcc atagaaattt gtaacttagc cattttctcc acctctttct gacaaacaca
     1981 ttatatcacg gaaagagttc aaatggaatt aatacaacta cttttgaact ccttgaaatg
     2041 cagtgtattt atctaaatat aaggaaagga tatagtgcta aatggccttg atagatagaa
     2101 aagtttcaaa atccgccaca gcgataattg aagcgaacac tcaacgacac acagaacagg
     2161 aaaagactcc taaaagaacc aaattaacta ggacgtgtct gaaaactcca caaagatgtg
     2221 gtatgctctg gtaaaaaacg aatggcacaa ccacgaagac cgatatggtt cttggaaaac
     2281 tttctccacc ctttttgtaa ttggagggat agtggtttat tgatagcgat tttccaagtt
     2341 cttcaagtgg aaccagacat tgagcaccac ggctaaaaag tgaatgaagt ctttattttt
     2401 aatcaataat ttttaatgat taccagtaat tggttttttc atgcgcccta tttctatttg
     2461 atgtggagtt aaataatgct gataagtaaa aatttttccg tcaattaaac tgccactagt
     2521 gaatcctgtt ctattggtaa aaagagaata gtctgggtat tcatctacac caaatgataa
     2581 agattcagcc ataaactgtt ttttaccagc aaattcagca ctgctacctt gatggatatg
     2641 tcctacaaaa atacctaaaa tatcactttc ggcaatcaat tcagcaaaac cttccggaac
     2701 ttcagttaca atagatggat cttcccacga caatggatga tgaaccaata aaatcgttcc
     2761 cttttcagca ggtgtaccca accactcttg taattgaatc atctgtccta tcgatatttt
     2821 tccttcgtgc tgggtatctt gagcagaatc caaggaaata attcgtaacc cattttgatt
     2881 cgtacaatgg atatactctc ccgtttcatt gctttcaaca ctcattcctt caaagaaaac
     2941 actgcgacga tcatgattcc cacgacaaaa atagtaaggt gtggttggga aatattgatc
     3001 caacattttc cgtaagactt gataatcaga aatcccccct tcatgtacta aatcacctgt
     3061 taatagaata aaatcgtaca ctgttaacga gagttcttgt aacaattttt ccaatgccaa
     3121 ctgtggattt ttaattgttg gaaatagttc agcagtaaac aagttagttt gatagttttc
     3181 tctaaaatgg atatcagaga gatgcaaaaa gcgcatggct attccctctt tcttcaataa
     3241 aataatcagg atatcctaaa ttttcttgcg tctgataatc aaaaaaatgc attctttcag
     3301 gttctatgcg gagattgact gcatcaccaa tctgccaagt aggtttttca ttaaggacaa
     3361 tcatttctat accatcgact ttaacggtgt acgcataact ttgacctaat agctcacgat
     3421 acttcacttg accgtgtaaa tcgcttgttt cttttgaaaa ggttacgtgt tctggtctta
     3481 tgcctaaata caatgagcca ctaacctgaa gaagttgttt ttgccaagct ggattcaatg
     3541 ttaaaaattg actggctacc tttaatgttc cttgatcata agtaactggc ataatattca
     3601 tgcctggcga accaataaaa tttgcagtaa aagtattttt aggtttcgta taaacatttt
     3661 tcggcgtatc aatcatctga attctacctt cattcaataa aacaatccgg tcagctaacg
     3721 tcatcgcttc aatttgatca tgagtgacgt agacaaaagt ttgtttataa cgttgatgaa
     3781 tttttaccaa ttcttttcgt gcatccaatc gtaaacgcac atccaaattt gataacggtt
     3841 catctaataa aaaataatta ctccgtttaa ccaccgcacg agccaatgct acacgctgcc
     3901 gttgccctcc tgacaaatct ttaggaagtc gatcttggta tggcgttaaa cctaacatct
     3961 tcaaagcttc ttccaaacgg tgctgaatgt cagctgtctt aattttattc gcccttaatg
     4021 caaaaataat attctccttt acagtcatgt gaggatataa agcataattt tgaaaaacca
     4081 ttgcgatctc tcgttctcca caaggtagct tatttgctag ttgtgtacct aaaaaaagct
     4141 ctccactcgt aattttttcc aaaccagcaa tcattctcag caaagtagat tttcctgaac
     4201 cagatggtcc cagtaacaca attcgttctc cagcggccac agctaaatca atttctttta
     4261 aaatggtatg ttctccatat ttttttccga tatttcttaa ttcaagcgtt tctcccatga
     4321 tttgtcctcc aatttattta atacctgcag aaacaaaagt cccgataata tatttttgaa
     4381 aaattgcata caagactaac ggtacaaaca ttgtaattaa actaatcgcc attgctacgg
     4441 taaaatttgt cccaccttca gaactaatat aggtctgcat cgccaacggc aaagtataat
     4501 tatctactgt ccgtaaaatt aataccggcc aaacatactc attccaagag gtaataaaaa
     4561 accaaatacc ggtagaagta atcgcatgtc tagtaagagg aaaaacaatt ttactcatga
     4621 cggttgtttg tttgatgtta tccaatgccg ccacatctag aaggctcact ggaatttccc
     4681 gcattgtttg cgtcaataaa aaaatgccca tcgcatcagc aaattgtggt attatcacac
     4741 cccaagtagt atcaattaaa ttaagttttg caatcaacaa ataattagga atcatcgcaa
     4801 cagtaaaagg aatgaacatc gtaccaatga tgataaagta ccagattttc ttccctttga
     4861 ttcggcaata agtaaaacta tatgccgcaa aaaaagctac gcctaactta acaaccgtta
     4921 taattgtagc aataaaaaaa gtatttctcg taattttgat aattggaaga ttttcgaaca
     4981 aatagcaaaa attttccaag gtaaacggct gtggtatcaa actagtaatc gtctgatagg
     5041 catctttcaa tggcttaaaa gaattggcca taataaacaa taatggatag ataataatga
     5101 ttatgatgaa cataaataag agatgtggca accagcgttt aattttcata gtaaaccccc
     5161 ttttccacaa atttaaatat tacccctaag aaaacagcaa atattaataa agttaaaact
     5221 gaaattgctg aagattctcc gactttatac atttcaaaag ccttttgata agcataatag
     5281 atcacgtttg cactaccatt attcggtcca ccacgagtga tgatcttaat cggagtaaac
     5341 acatactgca gaccttgaac aatggtcatc accaataaat atagtcctgt cgaactactc
     5401 ataggcaaca caattgtaaa aaaaatgcgc cagatgggta cacgatccaa cttagctgct
     5461 tcgatcatct cttgatcaat tcccacaatt gctgcaaaaa tgacaatgaa attataacca
     5521 aaaactttcc aagcagtaat acctgcaatc acagcaatta cccaaccttg taaattcatc
     5581 cacataggca gtcttaaacc aaaatttccc aacaagacag caattggtcc cgataccgga
     5641 tttaatatcc atgtaaaaac catccccgcc actaccaaag aaatcacagc tggaataaaa
     5701 aataatggtt tatacaaatt ctgccctttt tctataacta ttcctaataa aaaggatgta
     5761 atatatggga acacacagct cagcaagaca aatagaacgg tataggacaa agtattcttc
     5821 aacactttat gaaagactgg atcagtcagc acctttacat agttggcaaa gccaacaaat
     5881 tctttagtgg gggccaccat attccagtca aaaaaactca aatagatcgt ataaatcaat
     5941 ggataaaaaa caaacacgcc caacaaaata attgctggcg ccagatacaa ataacctaca
     6001 tagcgcttca tttccgacag cctcctcatc cttatgtcaa aagcagacga ccttgttttt
     6061 cctttcttac aggcgctgtc gtctgcttgc tttgcttatt tgagtaactc attaatttcc
     6121 ttttgagtag ctttcatcgt ttcattgacg gaattacctg ataaaatttt atcccgcata
     6181 tccaacaatt tttgttcagc ttctaatcca gcatctccag gaaatgaact ccaactgacc
     6241 atcttatcca tctgatcaat tgctggtatc atcatctgat tttcagctaa gaaagctttt
     6301 aatccatttt gggcgtctgc aacatcttta cgtggtggta aataaccagt accttctgtc
     6361 catttagcta aagattcaac actataaaga tattttaaga aatcccaagt tgcttccttt
     6421 ttcttatccg tttgtgatgt aacagctaac attgcgccac cagcaggtag tttaactttt
     6481 ttcccttccc aacttggtga ctcaactgct ttagcagtaa aagtactatt tttttgaatg
     6541 ttgtcccttt gagcaatagt ggtatagacc atcgcgacgt taccatcaat aaagctttgc
     6601 acaccttgtt cccacgtagt gtgtaatgcc gtctggtctt ttaccatcct ttgatataat
     6661 tcataagcag cgataccatc tttagaagca aaggtagctt tgccatcttt tattacactt
     6721 gccccattac tttctaaaat tgcttgttgt gcccaattat cagcaggttc ttgaatatac
     6781 acgccatact ttccagtttt ttcttttatt atcttagaaa aagtttgaac ttcttcccaa
     6841 gtttttggac cattttcatc caagcccgct gcttttaata tatcttgatt taaatataaa
     6901 accggattac taatggagta aggaataccc acttgtttac catctttttc agacagtgcc
     6961 atgatattgt ctaaaaaatg atctttaaaa taatcagcat tatccggatc cacttcttct
     7021 actaaagatt gaggattagt atatttgaaa ttttctgcaa aataatcttt aaaagcccat
     7081 cccacttgta ctacgtccgg tatttctttg gaggcaacag aagcttgtaa attttgcatc
     7141 aagcctttat acatatcagg attatatttt gctacaacct tcactttgtc ttgagattga
     7201 ttgtatttat ctactaattc attcactgtt tttccaccta gggtatccgc attaacatgc
     7261 caatactcta tcgttacacg accctccgat ttttttgttg tcttggtatt cccactacca
     7321 caagctgcca aagagatgac acttaaaaca gacacaaccc ctataacaag atttttcttt
     7381 aacataacat taacttcctc ctaatttctt tatggccatt tttctaatca agaaatatta
     7441 tgaactacca atgtaaattt ctagttaatt ttctgtaaat aattcgaaat ttgtgcattt
     7501 cggggtacag ggtcactgga catatccatc aagctaagaa taaagccccc atatggtcat
     7561 gtatcccatc tccaaatctc ttaactcctc tgttttgatt ataaaatagt ttattctgtg
     7621 ctcctttctt ccataaatag gcccatgcac aacgtgaggt tatattataa acaccacagc
     7681 gcgcaaggta ctcaatcgtt tctagttcat tgactgtcca tacagaaaat gtcaatccta
     7741 gctgctgccc cacttcacgg gccaacttag ttaaccatcc ttcatgaata ttgacgattt
     7801 gaacgctttg ataggctaat ttctgtaagg tatcgatcaa atctgcttcg gtaatatccc
     7861 caagtttttt cccttcgaac tgattttcaa tattataaaa tatatgcttt ccccaaatcg
     7921 cagcccaaga taattgtaca tagccactaa gaacaatttg tggccagatt tgataggttt
     7981 gtgccagcga aaaaacggcc atttccaatc cctcttcttt taaatcacag ttaatggtca
     8041 tggttgcctt ctcattggcc actgcttgaa acacctgctc aagggttacc gaatctggga
     8101 caacttctta cctgtcatgg gctagtatta gcgtaccatc tgtttgctga cgcacatcta
     8161 cttcaaaaca aagctggcga tccaaacaaa agcgaagaaa tgcccagcta ttctcctccg
     8221 tattgtcact accagagtgt gcagtgatca tggtcatttt gacccctcct ttaattccgt
     8281 gattttctcc cacacgtcca ctataccgat gaagcgttaa tttcttgtta atttcttgta
     8341 aattccctgc gttttttcag taaactagtt gtaaatcttg catgctcctt cctacgaact
     8401 catgaatcgt cgattttgcc cttctagcta gtttcaccaa ttaagtagtt ctacattccc
     8461 cgcttttaac ctactacacc tttttttgtt ttcagctatt ccttcttctt gctgaaagtc
     8521 cttgaggttc tttcttacag gataccatca aggtgttcaa tggcttattc ccattgaatg
     8581 accgagactt actatcagat ttctgctcgt tgaaccaagt ctttgataca ggatttcttc
     8641 cgtcatcttc tcacaaatac caccttgcgt ttctctagcg attggtcgat gtgcaccctc
     8701 ccagtggact gtcactaccc ctttttgcta tgcctagccc acaaaaaaag ctgtcctcac
     8761 agacag
//
LOCUS       DAYYJC010000101         8494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000101 DAYYJC010000000
VERSION     DAYYJC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.103"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACHA9N_002335"
     CDS             complement(<1..149)
                     /locus_tag="ACHA9N_002335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9085766.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACHA9N_002336"
     CDS             complement(593..1714)
                     /locus_tag="ACHA9N_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFM9085767.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACHA9N_002337"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACHA9N_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFM9085768.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACHA9N_002338"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACHA9N_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFM9085769.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACHA9N_002339"
     CDS             complement(4304..5215)
                     /locus_tag="ACHA9N_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9085770.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACHA9N_002340"
     CDS             complement(5232..6239)
                     /locus_tag="ACHA9N_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9085771.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACHA9N_002341"
     CDS             complement(6236..8365)
                     /locus_tag="ACHA9N_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9085772.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACHA9N_002342"
     CDS             complement(8370..8480)
                     /locus_tag="ACHA9N_002342"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085773.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAYYJC010000102         8306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000102 DAYYJC010000000
VERSION     DAYYJC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.104"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACHA9N_002343"
     CDS             117..656
                     /locus_tag="ACHA9N_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085774.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1676..2845
                     /locus_tag="ACHA9N_002344"
     CDS             1676..2845
                     /locus_tag="ACHA9N_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9085775.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3318..3872
                     /locus_tag="ACHA9N_002345"
     CDS             3318..3872
                     /locus_tag="ACHA9N_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085776.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4045..4482)
                     /locus_tag="ACHA9N_002346"
     CDS             complement(4045..4482)
                     /locus_tag="ACHA9N_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085777.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4530..5477
                     /locus_tag="ACHA9N_002347"
                     /pseudo
     CDS             4530..5477
                     /locus_tag="ACHA9N_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5748..6183
                     /locus_tag="ACHA9N_002348"
                     /pseudo
     CDS             5748..6183
                     /locus_tag="ACHA9N_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6199..7032
                     /locus_tag="ACHA9N_002349"
     CDS             6199..7032
                     /locus_tag="ACHA9N_002349"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9085778.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7277..8302
                     /locus_tag="ACHA9N_002350"
     CDS             7277..8302
                     /locus_tag="ACHA9N_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9085779.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
BASE COUNT         2955 a         1181 c         1462 g         2708 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     2521 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     2581 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     2641 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     2701 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     2761 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     2821 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     2881 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     2941 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
     3001 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
     3061 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
     3121 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
     3181 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
     3241 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
     3301 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
     3361 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
     3421 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
     3481 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
     3541 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
     3601 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
     3661 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
     3721 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
     3781 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
     3841 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     3901 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     3961 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     4021 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     4081 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     4141 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     4201 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     4261 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     4321 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     4381 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     4441 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     4501 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     4561 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     4621 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     4681 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     4741 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     4801 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     4861 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     4921 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     4981 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     5041 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     5101 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     5161 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     5221 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     5281 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     5341 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     5401 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     5461 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     5521 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     5581 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     5641 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     5701 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     5761 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     5821 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     5881 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     5941 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     6001 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     6061 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     6121 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     6181 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     6241 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     6301 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     6361 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     6421 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     6481 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     6541 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     6601 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     6661 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     6721 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     6781 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     6841 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     6901 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     6961 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     7021 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     7081 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     7141 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     7201 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     7261 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     7321 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     7381 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     7441 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     7501 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     7561 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     7621 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     7681 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     7741 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     7801 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     7861 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     7921 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     7981 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     8041 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     8101 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     8161 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     8221 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     8281 aagtgaaaaa aatatcgatt aaaaaa
//
LOCUS       DAYYJC010000103         8209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000103 DAYYJC010000000
VERSION     DAYYJC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.105"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA9N_002351"
     CDS             476..1339
                     /locus_tag="ACHA9N_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9085780.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA9N_002352"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA9N_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9085781.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA9N_002353"
     CDS             3339..3860
                     /locus_tag="ACHA9N_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9085782.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA9N_002354"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA9N_002354"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9085783.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA9N_002355"
     CDS             5941..6759
                     /locus_tag="ACHA9N_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9085784.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA9N_002356"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA9N_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085785.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA9N_002357"
     CDS             7228..7806
                     /locus_tag="ACHA9N_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9085786.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA9N_002358"
     CDS             7901..8038
                     /locus_tag="ACHA9N_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085787.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2460 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaata
//
LOCUS       DAYYJC010000104         8105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000104 DAYYJC010000000
VERSION     DAYYJC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.106"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHA9N_002359"
     CDS             complement(296..1024)
                     /locus_tag="ACHA9N_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085788.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHA9N_002360"
     CDS             complement(1002..2576)
                     /locus_tag="ACHA9N_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9085789.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHA9N_002361"
     CDS             2805..3743
                     /locus_tag="ACHA9N_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9085790.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHA9N_002362"
     CDS             3755..4666
                     /locus_tag="ACHA9N_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085791.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHA9N_002363"
     CDS             4690..6162
                     /locus_tag="ACHA9N_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9085792.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHA9N_002364"
     CDS             6213..6824
                     /locus_tag="ACHA9N_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9085793.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..>8105
                     /locus_tag="ACHA9N_002365"
     CDS             6854..>8105
                     /locus_tag="ACHA9N_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFM9085794.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYD"
BASE COUNT         2574 a         1217 c         1608 g         2706 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatc
//
LOCUS       DAYYJC010000105         8011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000105 DAYYJC010000000
VERSION     DAYYJC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.107"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..334)
                     /locus_tag="ACHA9N_002366"
     CDS             complement(20..334)
                     /locus_tag="ACHA9N_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085795.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            620..859
                     /locus_tag="ACHA9N_002367"
     CDS             620..859
                     /locus_tag="ACHA9N_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085796.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1221..1481)
                     /locus_tag="ACHA9N_002368"
     CDS             complement(1221..1481)
                     /locus_tag="ACHA9N_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085797.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1666..2163)
                     /locus_tag="ACHA9N_002369"
     CDS             complement(1666..2163)
                     /locus_tag="ACHA9N_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9085798.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2293..3003)
                     /gene="budA"
                     /locus_tag="ACHA9N_002370"
     CDS             complement(2293..3003)
                     /gene="budA"
                     /locus_tag="ACHA9N_002370"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9085799.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3016..4680)
                     /gene="alsS"
                     /locus_tag="ACHA9N_002371"
     CDS             complement(3016..4680)
                     /gene="alsS"
                     /locus_tag="ACHA9N_002371"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9085800.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4888..5550)
                     /locus_tag="ACHA9N_002372"
     CDS             complement(4888..5550)
                     /locus_tag="ACHA9N_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9085801.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5560..6375)
                     /locus_tag="ACHA9N_002373"
     CDS             complement(5560..6375)
                     /locus_tag="ACHA9N_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9085802.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6636..7079)
                     /locus_tag="ACHA9N_002374"
     CDS             complement(6636..7079)
                     /locus_tag="ACHA9N_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFM9085803.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(7213..7551)
                     /locus_tag="ACHA9N_002375"
                     /pseudo
     CDS             complement(7213..7551)
                     /locus_tag="ACHA9N_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7539..7916)
                     /locus_tag="ACHA9N_002376"
     CDS             complement(7539..7916)
                     /locus_tag="ACHA9N_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9085804.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
BASE COUNT         2495 a         1641 c         1220 g         2655 t
ORIGIN      
        1 actaaaaggc ataattgctt taattatttt ttgacttctc ttcaaacaca agaagtagtt
       61 gtgtaataat tgcagaaata aggattccaa taatagatat aaaatttgac atggaaacgt
      121 ctataacatt aaaagaataa agtgttgaag aaagtaaact gattgaaata acccctccgt
      181 aaaaaatagt ctgtgaatat acccactttt tttcagaaga cgtacttcgt ttggttctaa
      241 aacctatccc tgcatttcta ctcttatcaa aaaaccaaat atttagaagt aataatattg
      301 ctattattaa gataaaatta ctgtaaaaat ccaaaagcta tcctcctagc aactatttaa
      361 atacccaaaa aaattacctc tagataatat catacctacg ttttcactta taaatttttt
      421 cataaaaaaa gcgtttagag gcataatcgt taatacagat ggtaaccaat gtatctggtt
      481 tgaactggga tatcaccctc tccatatatg tattactaag actaatagat actataaaag
      541 acttgatagt tttgcattga ttttgcctaa tactgagcat aggtgtatgc taaacgatac
      601 tataaggagt gtgtacataa tgaaaagaat tctgtttatc agtttatcaa atttattgtt
      661 acttactgct tgtgggactt ctgcatcaaa gaacatacct tcagatacgc aaatttcgaa
      721 tagtatcgat tcaacaattt ctacaaatga gaaaaattct ccggtagaaa cttccgatat
      781 aaaagtttct acctctgcaa aaaacagggt aaagaaactg tcagcacgtc tcctttattt
      841 caaacaaact ctgaattagc ttcttcatct tcttcagcaa ataatgatga tagtttcgcg
      901 tttactccta actaaatgca acctttacaa atgctttacg ataacaagcc tgagctaaat
      961 gatcctgata tcagttttgc attttttgaa atgattggat cggatgatct ttttaaaact
     1021 actagtttgt ctatccgaaa ataggacagt tctggcagta taggttttta tcgagtttct
     1081 ccaccaggcc tcgttacaga gacagattct ctaggcgaac cttttgaata aatcaaaaat
     1141 catgaacaat cactgccaac taaataaagt gccttcaaaa gcttggtctt ctcgcttttg
     1201 aaggcacttt atttaaatct ttatgaaact aaagtatgtt catatttgtt gctatagtta
     1261 tatagatatt ctgttgtttc caactcataa acgtgagtat attttgccac tacttttccg
     1321 catttttcta atatttcatt gcgatcgacg tcatgatatt cgtctacgca gatcacaaca
     1381 caattttcta atttactgat tacttctcca accacttttt gtttcaagcc aataggttgg
     1441 caaatatatt tttttcctaa aatgatagtt tcgttattca tatttcatcc atccttaatg
     1501 ttttttcatt attcaaaatg ataataccat taaaatatga ttgtgaaaat caataacact
     1561 tgtgatttta taaaaaaata aaaaaaccga aaaacagaaa tgaccactgt ctttccggct
     1621 ctttttattc ttgatttctc ttcttttttg tttcaataga cgattttagt tattaataaa
     1681 tgaaaaagca tttatattaa aatttgctaa taaaagatct acatattttt ctagaaatgc
     1741 ttgatcgatt tcatcatacc ctttcgttac tccgcaatcg atatctaaaa caccgattaa
     1801 attaccttct tttaccattg gcactacgat ttctgattgt gcggctgaat cgcaggaaat
     1861 gtaatttgca tgggttttga catcatcaac gatcaatgtt tcttgttttt cagctgattc
     1921 tccacaaact ccttttccca tcttgatacg tgtgcaagaa actcgccctt ggaacggtcc
     1981 taaaatcaat tcattaccat cgaataaata atatcctgca aagacagtct caggcaaagt
     2041 ctcattcaac aatgcagatg aatttgctaa atttgctaac cagttatctt ctgcttctaa
     2101 taatccttgt tgctgtaata ataaaatttc gtatgcatct tttttctgtt tttctgtcca
     2161 catgataaaa accgctcctt tttgatctat ggagaagatt atagctcagt ttttttgtaa
     2221 acgctaggtt tcctcctcaa aaaaacgaga tacgtcaggt taatcacctt gaacgcatct
     2281 cgctgtcttt ttctattctg cttgttggat ttctgctgct aaatcagcat aatcaatcgt
     2341 ttcgtgaaca aaagtcggat catcaactgg aaaatgctgt acaagggacg agatgcggct
     2401 aatctcaact tttccttcag ccacttcaaa atccattaca tgtccgccaa atgtatgtgt
     2461 atcatcgata aaatgcaggt gaaatcccgc tgcagcaact ccttggaata attcaggcgt
     2521 aaagaacccg actaatgttc cttggatatt gctttgttgg aattctggtt gagactctga
     2581 aatttttgct agtcttctgt aaggtttttc ttgcttcggc atgatacgga tgtgcatatt
     2641 tttgaataat ccagttatct tcactgccaa aaataggttt tccccggctt ctcttactaa
     2701 tatgtctttt ttcaattgtt ctccatctgc agtttttgag acagaaaaac tagtatctgg
     2761 cgtaaaatct gtaattgctg catagggggt aagttcactt ccatctaatt gggcaactga
     2821 gccatctgat cttccttgat aagctacgcc atctaaaaag atgacttcac catcaagccc
     2881 atgcaatgtt cccaatccca gagttccttt ttctaatata tgagcaatcg tttctgttcc
     2941 atccatcaag ccggccatca aagctcctaa tgtcccgtgc tggtataaga ttttttcagt
     3001 catgtcgaca cttccttaat agaattgatc tggtaagatt gtttctccca atttctcatt
     3061 atcacggtaa tcaatcggaa tatcaataat tacgggtcct tctgtcgcaa atccttcttc
     3121 taaagctttt tctaattcct ctacacttgt agctcggatt cctttagcgc caaaagcttc
     3181 tgcatatttc acaaaatcaa ctggaccaaa gtctacacct gacgcccgct gatatttcat
     3241 tttttcttgg aactctacca tattgtagtg tccatcattc caaatcaaat gaacgatatt
     3301 caatttttta cgtaccgccg tttctaaatc ctgggcagag aataaaaatc ccccatcgcc
     3361 ggaaactgat acgatttgtg tatttggacg caccaatgca gcagaaatcg cccatggcaa
     3421 tgcaacacca agcgtttgca taccattact gaataacaaa tgacgaggtt cataacttct
     3481 aaagtggcgt gccatccaaa tataatgact acctacatct acggtaacag tcatatcatc
     3541 tgttactttt gactgaagcg tgttaataac ttctaaagga tgcaagatac ctgcttctcc
     3601 ttttgattga acaatgtccc gctccgttaa ttttgcttga agagatgcta aatattcttt
     3661 tgcatcttct gacacttgtt gaggctctaa tgaacctgtc aataaatcaa gagtggctga
     3721 gatatcaccg atcaattctc tttcaggctg cataaaagga tcgatttcag ctggcgcttc
     3781 atcgatcaca atgatccgcg catctttttc cgcattccag tttcttgctt catattcgat
     3841 tggatcatag ccaattgcaa taactagatc acttcgtttc aaaagcatat ctcctggttg
     3901 gtttctaaat aaacccacac gaccaaagaa atgcgcttct aattcacgtg agatcactcc
     3961 agctgcttgg aatgtctcta caacaggcaa tgctgtcttt tcaactaatt gcctgatggc
     4021 taacgtttct ttttcacttg atcctcgcat acctactaat agtaccggta gttttgcttc
     4081 tctaatgcgt tcagctaggt aacggatatc atctgcagaa gcagaaccta atttaggatc
     4141 gctcaatggc ttgatgacat taccttggac aggtgcatcc actacatctt gcgggatact
     4201 gataaagctc gcgccttttt ttgaactctt agccatacga taagcattgg caatcacttc
     4261 tgaaattgtt tctggatctt ggatttctgc actgtatttt gtgattggct ggaacagtgc
     4321 tgcattatcc atgctttgat gggtcaactt caatagatcg cttcgtttta cttgtccggc
     4381 aattgctaaa actgggtcac cttctgctgt cgctgttacc aaacctgtag ctaagttact
     4441 agcaccagga ccacttgttg cgataactac tcctggctct cctgtaatac gtccgattgc
     4501 ttgtgccata aatgcagcat tttgttcgtg acgagtcacg attaattcag gaccttgatc
     4561 ctctaattca ttaaatacac catcgatttt tgcacctggt ataccgaata cataatcaac
     4621 atgatgatta atcaaacttt ctactacaga tttcgaacct tgtttcattt tttcagccat
     4681 tgaacatcgt cctttttatg ctttattttt aattattagt tagaaataat cacaaataag
     4741 tgatacagtt tctaaacctg tttcatacaa aagtatagca cgttttattt catttgttta
     4801 gccatatgtt tcaaaaaatg aaaaagaatg acagttttta cataaaaaaa actgtcattc
     4861 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     4921 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     4981 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     5041 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     5101 tctcacctga aaataccagc agttggtgct aaaggaaact gataaacaag caagtaaaac
     5161 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     5221 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     5281 gcgaaacatc tttatgaaca aacgtactgg tgaaaaacta ttttcttttg ctaattcttc
     5341 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     5401 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     5461 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     5521 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     5581 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     5641 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     5701 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     5761 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     5821 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     5881 ctcacataaa gaatctggga tactactgta atattgcttc aacaagaaca caccaaaagc
     5941 attgaacgct tgtaatagca ccaacgacca caacgtatct gttaaaccca cttttctcat
     6001 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     6061 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     6121 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     6181 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     6241 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     6301 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     6361 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     6421 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     6481 gctgattcat ttccgttctt cttatttttg taagcgctta tttaacaagt tatgtaaatc
     6541 tctttatttt tttgatttaa aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc
     6601 ttttgtattg aaatttcttc gattagagat acttattact gttcaaaagc ttctttgaaa
     6661 cggtgaatca attcattgac tacaggtgat aaaatccgat tctttttcca gactagcatg
     6721 cagttcgttt caatcattgg gtcaagcgga acgaatgtca ctcgctttga atgatgactt
     6781 acagcacctt ctatgactaa agcagcacct acctgattct caattagcgc aaatacattg
     6841 aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct tttttatgcc taaccagtca
     6901 gcaaacattc gctgtatttc tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct
     6961 tcttttgtta tcacgtcttt ttttgctaaa acagaatttt ctaaaatgag caatccccat
     7021 ttttcctttc ttggtaacaa aatcgtttcg tatttctctg tatctactgg atctagcaaa
     7081 atcgctaaat ctaacaggct tttttctagt cactccgtga tatcatcact tgttccgctg
     7141 tggatatgaa attgaacgct gggataatca cggatcaact tttctagtaa tagtgccaat
     7201 gtattcgaac tatcagcttc cacacagccg atcgtaaatc ggccgctgaa taattgtttt
     7261 tttctgttct cgaattcttg agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg
     7321 cttttttaga aaataacctg cttctgtcaa gatcattttg cggttttcgc gtacaaacag
     7381 ttctgttcct agttactctt ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag
     7441 atgtagaact tctgcggctt gagagattgt tccttcttct gcaattgtcc aaaaatattt
     7501 tagtaagcgt aactccatag cgatgctcct tgattttttt acttgataca ttccatttta
     7561 tttctagata acacacgatc atatttcttg atttttcctt gtaaacgttc caaacattct
     7621 tgcatttcag caattttttt cgtcaaaagt tcactttctt caatcaaaag cttacgccgc
     7681 tcaaatacag ttgcttctcc ttcgctagat aggcgaatat attcaatcaa agactcgatc
     7741 gataatccgg catttcgcat acagatcgta aagtcgaccc attttaggtc ttcttctgta
     7801 taatcacgga ttccgccttt tgtccgatta actggtggaa tcaacccgat tcgttcataa
     7861 tagcgcaatg tatcggccga aatgcccttt tcttcactga cttgtttgat attcatctga
     7921 tcactctttc tcttaatttc ttgccacttt acttatgtat tttatttatt ctacaacttt
     7981 aagtgtactc caagtcaata ctcatttcta t
//
LOCUS       DAYYJC010000106         7999 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000106 DAYYJC010000000
VERSION     DAYYJC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.108"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACHA9N_002377"
     CDS             complement(21..995)
                     /locus_tag="ACHA9N_002377"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9085805.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACHA9N_002378"
     CDS             complement(1075..1950)
                     /locus_tag="ACHA9N_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9085806.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACHA9N_002379"
     CDS             2080..3987
                     /locus_tag="ACHA9N_002379"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9085807.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="ACHA9N_002380"
     CDS             complement(4528..7806)
                     /locus_tag="ACHA9N_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9085808.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2287 a         1668 c         1477 g         2567 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagag
//
LOCUS       DAYYJC010000107         7984 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000107 DAYYJC010000000
VERSION     DAYYJC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.109"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..795
                     /locus_tag="ACHA9N_002381"
     CDS             94..795
                     /locus_tag="ACHA9N_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9085809.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1404..2420)
                     /gene="tsaD"
                     /locus_tag="ACHA9N_002382"
     CDS             complement(1404..2420)
                     /gene="tsaD"
                     /locus_tag="ACHA9N_002382"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9085810.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2417..2881)
                     /gene="rimI"
                     /locus_tag="ACHA9N_002383"
     CDS             complement(2417..2881)
                     /gene="rimI"
                     /locus_tag="ACHA9N_002383"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9085811.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2888..3430)
                     /gene="rimI"
                     /locus_tag="ACHA9N_002384"
     CDS             complement(2888..3430)
                     /gene="rimI"
                     /locus_tag="ACHA9N_002384"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9085812.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3414..4136)
                     /gene="tsaB"
                     /locus_tag="ACHA9N_002385"
     CDS             complement(3414..4136)
                     /gene="tsaB"
                     /locus_tag="ACHA9N_002385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9085813.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4327..5307)
                     /locus_tag="ACHA9N_002386"
     CDS             complement(4327..5307)
                     /locus_tag="ACHA9N_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9085814.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5331..6815)
                     /gene="galT"
                     /locus_tag="ACHA9N_002387"
     CDS             complement(5331..6815)
                     /gene="galT"
                     /locus_tag="ACHA9N_002387"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9085815.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6827..7816)
                     /gene="galE"
                     /locus_tag="ACHA9N_002388"
     CDS             complement(6827..7816)
                     /gene="galE"
                     /locus_tag="ACHA9N_002388"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9085816.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2250 a         1706 c         1411 g         2617 t
ORIGIN      
        1 aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa aagtgacaat
       61 aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat cgattttcac
      121 ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga gttccaacag
      181 atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt cgacgatggt
      241 gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga acgttatgat
      301 gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt tcctatgtca
      361 ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga tattgaaaca
      421 ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc
      481 tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat ccaaatcgga
      541 ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa agaagaattg
      601 ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt aaaagaatta
      661 gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca actacgtgat
      721 tccgggctgt tagattatga caaaaagcgt tttactttct gtaagacagc tactactcca
      781 gctatgagag aataacgcga acaaaaataa gcagtgaccc tgataaatat cagggtcact
      841 gcttattttc ctccctaaaa cttctgaaag atccggcagt tttagatcac tggacaagac
      901 tcaaagattt tctatgtgtg tttcagttat gtaatattca ctgtttgcaa aaaatactgt
      961 tagattaact agatgaagcg tcttgtcctc atccgatcta gattattcga tattcctcga
     1021 caataaacga ttactcagtg agggcaatca gaggaacgga taatgataaa taagctgtaa
     1081 aaaatagcaa aataacaata atgattaaaa gtatgtaaaa aatagggctt tgacaaaaac
     1141 gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc aactcttcgg tcacaagtca
     1201 taatgttcat aaacatgagg agctgttttt tgaaattgtt ccttgtgaat cagagttaaa
     1261 cgacaggctt acgaactctt gattaaacgg cgttcaaaag cttgcatacg cggtaataag
     1321 aacagccagg tcaaaaacat gagaagccgt ttttcctggg ctgttcttat tacttgatgc
     1381 agggcagctt tttcacaacc tctttattca gataaaacca agcttggatc agcattcaac
     1441 tgatagctag caaaatttct tttttgcatt tccacatgtg ctgctgctcc aatcattgca
     1501 gcattatctc cacaaagtcg aagaggggga atcactagtt ctacctcagg gagtttagct
     1561 gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc tccagctata
     1621 atcagttgct tgactggata attttgacaa gctcgaagtg ttttagtaat caatacatcg
     1681 atcacacttg cttgaaagct agctgctaaa tcatttttgt ctagatcttc tccacgctgt
     1741 tgtgcattat gcaccaaatt gataaatgca ctttttaatc cgctaaaact gaaatcataa
     1801 ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat cttttccctg atgagctagc
     1861 tgatcgattt cttttccgct tgggtaagaa agacctaata ctcttccaac tttgtcataa
     1921 gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc atcttcttgc
     1981 atatagacta attctgtatg tcctccgcta acaagaagtg ccatcaatgg aaactggaat
     2041 ggtttaacca gacgggctgc atagatatgc cctgccatat ggtttacagg aataagcggc
     2101 aattggtgtg cccaagcaaa agccttagct gcagaaatac caatcagcaa cgagccgaca
     2161 agtcctggtc cataagtgac agcgactgcc gataaatctt ctgccgacac accagcttct
     2221 accagtgcat cttccaagca taaggtgatt tgttctacgt gatggcggct agctacttca
     2281 ggtactacgc caccaaaacg tttatgactg tttatttgag aagcaacgat gttagataag
     2341 atctctgttc catttctaac gaccgctaca ctcgtttcat cacaacttga ttcaatcgcc
     2401 agaatcaatt cttcattcat ttcatcgtct ccgtttttaa ctttctgctc ataatgatcg
     2461 catcttccgt cggattgcga taatatgatt tccgtttacc taatatacga aacttttctg
     2521 attcataaag ttttcttgct gcttcattag atagacgtac ttctaaaaaa acatgcagcg
     2581 aatcttttat ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc ccttttcctt
     2641 gttctgaaac tgcaacagca atatttgtga tctcgacttc atctattact ttcgagtatc
     2701 caataaagcc acagatctgg ttatcttcag ctaaaagcag ataatcagag tgaggctgtt
     2761 ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc atgtggataa gcattttcgc
     2821 taatcatcca tacgcattct gccaagtacg cttcatcaaa atcttttttt ctgcaaatca
     2881 actaatctca ctcattttct aaaagtttta tcatatccaa cgcatcttct tttgtttcag
     2941 tgtaatatct ttttttgatc gttgttgctt gaaaccccaa tttccgataa agtctttgtg
     3001 catcttgatt actcatacgc acttctaatg acatcgtctc acagcgattc attatagcaa
     3061 agttctcgat ttcgtcaata agcagcgaac cgattcgttt acgctgatat gccggtcgaa
     3121 cggcaatatt cgtgatatgg caatctaaac cgaacgctct tgaaccgata aaaccaatca
     3181 ttcttccatt atcaaagata cctagatata gatgaggaag cggtgacttc aattctgata
     3241 taaaggcact ttttgtccat ggcaattcgc cattatagac ttctcgttcg acatccagca
     3301 gctctttgat atcttctgta gtcaattctc ttagtacgta acgacgatcg atttgttcga
     3361 ctgttttttc tgtataaatc attttatctt gaaaaaagct tctggcatac gctctaaatt
     3421 ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca accatttttc ttctgcttct
     3481 actcgtttta aatagcgagg aagaaaagct tgtttctctg caactggttc aactaaagca
     3541 cctaattgag ccacagttac accacttgga atatcccatg ctggaactgt attaatcatc
     3601 gcactaggca atatttcctt tatttcagag aagaatttgt gacaatcact cccgacgaaa
     3661 taaatagctt cattcagctt ttgtagttgt gtgaacaaat cagaagcaga aatatgtaca
     3721 tcttcaagta cagtttctaa tctattttct ttccagcggt atgccccagc gtatacattt
     3781 tttcttcgag catcaaacat gggcacaata atattatcca caccgatgca attagccgcg
     3841 atggttttca gactagatac tcctactagc tctttatcta atgtataagc aatcgttttt
     3901 gctgtcgtta cacctatacg aagccctgta taagaaccgg gaccatcaga aaccgcgatt
     3961 cggtcaatgt cttttggact tatttgaaga tctgcaaaca attggtcgat tgcaggcatc
     4021 agtgttacac tgtggttgcg tttgatattt gtttgtattt gtccaagaat ttgttcgtct
     4081 tctaccacac cgatcgccaa agtttggttt gcagtatcta ttcctaatgt aatcatatat
     4141 ttgatccttt ctgctatgac attctcgcct tccattgtag catacttttt tattctgagg
     4201 atgaaaatac caaaggcaaa actagtacaa taatacatga ttatctgctg gcgggcaaat
     4261 tttgcccctt cctgaaacac aaaaaagctg cggtatcact ccattttccc acagcttttc
     4321 catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa tctttcttga
     4381 aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct ctcccatcca
     4441 ttcggtataa acttttacag tagaaagcga gggcgttaca tatttcgcta cactgacatc
     4501 attgaagccc atcacagaaa tatcttctgg taccactaaa cctttttctt gaatcgcttt
     4561 cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa catgcggatt
     4621 cgtttttaaa tattgcttca ctgcttgata tcctgactcg acagaaaaat ctgcttcgat
     4681 aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca taattctggg
     4741 atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca tagcgatatg
     4801 ttgcgcttta tgggagacaa agtgttcaat gactttttga acacttgact taaagtcgac
     4861 aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca aggcattttg
     4921 ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga tcgttccatc
     4981 tgccggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt ttgcttcttc
     5041 tgcctttttt tcgattccta ggcgaataga taaataataa agatcttcaa gctcttccgt
     5101 atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat ttttacgatg
     5161 tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg ctaagccaac
     5221 ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa ctcctgccaa
     5281 ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt ttataaaatc
     5341 gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac tccagcgtct
     5401 tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt gaccgtatct
     5461 ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg atattcagcc
     5521 atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg tttcaaacga
     5581 ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt tttgatatgt
     5641 tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac attgttgtct
     5701 cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg cgtaatcgta
     5761 tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc cgaatatgct
     5821 ttccattttt ccaatactga gttagctgct tcaataagca agtttttctc tgttgattgc
     5881 aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg ataatcttct
     5941 agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg tcctgcctga
     6001 taatgatcat gagaaagaat cgatcctcca acaattggta agtcagcatt cgaaccaaca
     6061 aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc ctttgtgatt
     6121 ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata agcataaggg
     6181 gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg gtgattggtg
     6241 cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag catgcaggta
     6301 ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc tttttctggc
     6361 ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg aaatttgatg
     6421 ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt aaagaaatac
     6481 tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt gacgactgaa
     6541 ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc tgctggcaat
     6601 tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa gtctacagat
     6661 tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac catccctaaa
     6721 agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc gatcgcaagt
     6781 gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat tcttcataac
     6841 catttggatg gctgacatgc caatcccatg ctgttttgat aatcgctttg atatctgtgt
     6901 attcaggttt ccagcccaga atctctctcg ctttttcact tgaagcgact aatcggctag
     6961 gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc acttctctag
     7021 ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg aagaagttgc
     7081 tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc aaatcttcta
     7141 cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct ccataaacag
     7201 ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga tgcgtttctg
     7261 gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg aagtagcgca
     7321 aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt tccatcatca
     7381 atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg attggagact
     7441 ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc acgttgaact
     7501 cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa tacatcaatg
     7561 gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact ccttcaatcg
     7621 gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct tcataaaaat
     7681 gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc acagctactt
     7741 gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg gctccgccta
     7801 gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt gttgattcta
     7861 tataatttaa aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa aatttcgtaa
     7921 attcatatgg taaatttata ataaaacatt tagtaaacat tttcaagggt tttttgcaaa
     7981 ggtt
//
LOCUS       DAYYJC010000108         7694 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000108 DAYYJC010000000
VERSION     DAYYJC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.110"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            413..619
                     /locus_tag="ACHA9N_002389"
     CDS             413..619
                     /locus_tag="ACHA9N_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9085817.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="ACHA9N_002390"
     CDS             complement(821..1822)
                     /locus_tag="ACHA9N_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9085818.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="ACHA9N_002391"
     CDS             complement(1827..3740)
                     /locus_tag="ACHA9N_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9085819.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="ACHA9N_002392"
     CDS             complement(3908..4414)
                     /locus_tag="ACHA9N_002392"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9085820.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="ACHA9N_002393"
     CDS             4574..5014
                     /locus_tag="ACHA9N_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9085821.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5173..6513
                     /locus_tag="ACHA9N_002394"
     CDS             5173..6513
                     /locus_tag="ACHA9N_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9085822.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6582..7610)
                     /locus_tag="ACHA9N_002395"
     CDS             complement(6582..7610)
                     /locus_tag="ACHA9N_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085823.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
BASE COUNT         2196 a         1553 c         1238 g         2707 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct
     5041 ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta
     5101 ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa ccattcaaag
     5161 gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta
     5221 aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga aacatgtttt
     5281 gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca ctatcaaaat
     5341 aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa tgatgtcagt
     5401 aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag
     5461 acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg cagagtcaaa
     5521 caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc acgtatgaag
     5581 cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccgtttct
     5641 ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt gaaaaacgtc
     5701 tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg
     5761 gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag aaaagtccgt
     5821 gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag
     5881 tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca catcggtcgg
     5941 gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg
     6001 aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt gaatggtcag
     6061 catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat agtcgatcgg
     6121 ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt ttttctttcc
     6181 gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag
     6241 ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac agaaatcaaa
     6301 cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa ccatatcaaa
     6361 gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga acgaatcttt
     6421 ttatatcgag gaaaatattt caagaaaact aagaacacta cccaactgac taaagccaga
     6481 acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt
     6541 gaatttcttc accaacactc attgacgaag agccgctttt cttaattaac gaaatccaat
     6601 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     6661 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     6721 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     6781 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     6841 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     6901 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     6961 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     7021 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     7081 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     7141 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     7201 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     7261 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     7321 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     7381 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     7441 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     7501 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     7561 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     7621 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     7681 aaatgatagt tttt
//
LOCUS       DAYYJC010000109         7593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000109 DAYYJC010000000
VERSION     DAYYJC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.111"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACHA9N_002396"
     CDS             complement(59..787)
                     /locus_tag="ACHA9N_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9085824.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACHA9N_002397"
     CDS             complement(800..2080)
                     /locus_tag="ACHA9N_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9085825.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACHA9N_002398"
     CDS             complement(2221..2823)
                     /locus_tag="ACHA9N_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9085826.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACHA9N_002399"
     CDS             3068..3274
                     /locus_tag="ACHA9N_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085827.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACHA9N_002400"
     CDS             3586..4305
                     /locus_tag="ACHA9N_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9085828.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACHA9N_002401"
     CDS             complement(4386..5069)
                     /locus_tag="ACHA9N_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9085829.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACHA9N_002402"
     CDS             5287..7473
                     /locus_tag="ACHA9N_002402"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9085830.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2720 a         1282 c         1427 g         2164 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cac
//
LOCUS       DAYYJC010000110         7533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000110 DAYYJC010000000
VERSION     DAYYJC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.112"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..360)
                     /locus_tag="ACHA9N_002403"
     CDS             complement(70..360)
                     /locus_tag="ACHA9N_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085831.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(478..738)
                     /locus_tag="ACHA9N_002404"
     CDS             complement(478..738)
                     /locus_tag="ACHA9N_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085832.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(875..1288)
                     /locus_tag="ACHA9N_002405"
     CDS             complement(875..1288)
                     /locus_tag="ACHA9N_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9085833.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1744..2229)
                     /locus_tag="ACHA9N_002406"
     CDS             complement(1744..2229)
                     /locus_tag="ACHA9N_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085834.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2368..2583)
                     /locus_tag="ACHA9N_002407"
     CDS             complement(2368..2583)
                     /locus_tag="ACHA9N_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9085835.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2807..3607
                     /locus_tag="ACHA9N_002408"
     CDS             2807..3607
                     /locus_tag="ACHA9N_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9085836.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3626..5494
                     /locus_tag="ACHA9N_002409"
     CDS             3626..5494
                     /locus_tag="ACHA9N_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9085837.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5487..5978
                     /locus_tag="ACHA9N_002410"
     CDS             5487..5978
                     /locus_tag="ACHA9N_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9085838.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5966..6520
                     /locus_tag="ACHA9N_002411"
     CDS             5966..6520
                     /locus_tag="ACHA9N_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9085839.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6538..7533
                     /locus_tag="ACHA9N_002412"
     CDS             6538..7533
                     /locus_tag="ACHA9N_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9085840.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2682 a         1208 c         1432 g         2211 t
ORIGIN      
        1 aagataacac ccaactttca aaaatatgtt ttggaagttg ggacaaacga gttaatacat
       61 tttaatacat tattttttat tcaaaaaaaa tagcacaatg gcaaagatcg taagagagat
      121 cataagcccc aatgaatgac taaaaagaaa agcagaatca catgccatat catcccatat
      181 ttcttcaaga aaataaataa gcgggaaaaa gggaaggaat agtagaacat cgatccagca
      241 ccattttttt cttcttttga aataacataa aacaataaac atgaacgtgc agggaagtaa
      301 aaacaaagct ttactcataa ctaacaaaat accagctcct tttatttttt tctcccccac
      361 attcaaagag taacaatggt atgtacttaa taatagaaaa atggatcaac ttgtatgaat
      421 aacagctgaa aagatctgca ccaaataaaa aacttccagc caaaactgac tggaagttta
      481 tttttttctg aaatattcta acacaaatat gaaaaagatg gccgaagcta ataagccgat
      541 ttcgtagcaa aacaatgaga taccagagtc gtaataataa aatcctctaa tcttatggat
      601 aaaatagatc atcggacaag cagataagag taagtatttg ttaggccttt tgaataagta
      661 aaagaaagca ctggtcatta tgataaatgg tatccaaaac aaaagtttac ttaagacaat
      721 caaatgatca actcccatat tttttaccaa gagagatcat aacatcttta tcgtaaaaac
      781 aaaatatttt tttgctcatt ttgaaaattt aaacatactc gttcttatcc aaatatataa
      841 aaatcgcccc tcattcatcg agaggcgata agttctaatt taactcaatt ttttttgttt
      901 cttttggctg ataagctgtt ttaggtaata ccaatttcag cacaccgttt ttaaaggttc
      961 cagtgatttt ttcttcatca acattaggaa gatagaatga acgtttgtaa gagaccgaat
     1021 gtctttcttt ccgtacatag ttgccatctt ctttatcttc tgtatgggac tcatgattgg
     1081 cggagatcgt caaagtatta tctgaatatt caactgtcgt gtcttctttg tcacagccag
     1141 gcatatcagc agttagtttg tattcttttt cattttcgac taaatctact tttggataac
     1201 ttgaaacacc taaaaaatca ttgaatgctg gactaaacac gtcattgaaa tcagggaaca
     1261 tgtcgcgaat gctaggtaca tttgccattt ttgatcatcc tttcaataag tattttttat
     1321 ttatccctat ttacgtaata actctatttt gaaaaaaatg caagtataat ggttagcttt
     1381 gtgaatattt catctaatac gatacaaagg ctatctttct aatttgaaaa agggatcatt
     1441 aaaataataa gaagaaaata tgtcaaaagg taaaaaagta aattcttcta tccaaatata
     1501 gaaaaattga atctagaaaa ccgcccgaca ataaatttca ttaactcagt tgaccgaaaa
     1561 tgaagtagat accaaaaatt tatcaagaga gcaagtagag aagtgagtat attcataaga
     1621 gaaatagctg taacgaatta ttaggttaat gcaaatgctt atttatactc cgtataactt
     1681 tctcagaaaa aatgatacca ataatgactt gctagtactg taaatccgta taatatcatc
     1741 taatcaaaat ggtgttccat ttgcatctgt catcgtaatc gatccttcgg gtgacactcg
     1801 ataaaaacca acgcttccag atcctccttg ggaacggatt tgtttgctgg aagctgtgaa
     1861 caaataatct cgatcaattt tttgataaaa gctaataaca atatcgtcac tttgtaattc
     1921 ggaacggtgg gcaataagaa atagaagtgc tttgtcagag ttttctaatg gaagagaagg
     1981 tacagtgctt tcttgcattt cagatgttga agaaatgcta aacgactgaa tacttgattg
     2041 gctatgttgc gaatctacat taccagtaga agtattagta atggaagtag tagaagtact
     2101 tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa gattctttaa cagaattctt
     2161 tgttttatat ccacaggcag aaaaaagtat tacagttccc aacaagcctg cgtaaatccc
     2221 tttatgcatg atagctcctc ctaaaataga ttatctttat tcagtatatc acttaaacaa
     2281 agaaaagcag atggtttaaa atgaaaaaac cttcccaata aagggaaggt tcatgaagat
     2341 aataactttt ttaataatta tagctttcta atatctttca tcaacttttc tagtaatcat
     2401 tgggaaaaat ttcaagcagt agatcgcaca aatacctacg acgatataaa tgatcttcgc
     2461 aaaaatagtt gttgaaccac cagcaatcat agctactaaa tcaagttcga acaagcctac
     2521 caacagccag ttcaaccctc ccacaatcaa taaagttaat gcaatagcat ctaaagtttt
     2581 cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc
     2641 ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct
     2701 tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg
     2761 agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt
     2821 ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat
     2881 cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg
     2941 tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataaa
     3001 agaagggata tataaagtaa tgaaacattt taagacgatc gatggtctag tcaataatgc
     3061 aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt
     3121 atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag
     3181 ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc
     3241 tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat
     3301 gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg
     3361 tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct
     3421 ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac
     3481 gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct
     3541 ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag
     3601 aggataaagg atagggggga cgcacgtgac gctagtaaac agatggtacc aattattgaa
     3661 attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag
     3721 taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa
     3781 aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga
     3841 gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc
     3901 ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga
     3961 agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat
     4021 caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga
     4081 tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa
     4141 aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc
     4201 tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc
     4261 tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca
     4321 agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata
     4381 tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag
     4441 gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt
     4501 agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt
     4561 attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga
     4621 aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat
     4681 cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc
     4741 atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg
     4801 aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca
     4861 aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt
     4921 ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa
     4981 tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc
     5041 cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga
     5101 atatttaaac aaattagtcg ctcctttgat cagaggctat tatgtggatg aagaatttga
     5161 aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt
     5221 tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga
     5281 tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga
     5341 aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat
     5401 aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact
     5461 aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag
     5521 cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa
     5581 catatagcga actgcgaaga aaaggaaaag tagcgattgg taaacgacct gggaaaatcc
     5641 gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa
     5701 aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag
     5761 atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag
     5821 cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg
     5881 aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga
     5941 tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat
     6001 gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt
     6061 tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt
     6121 aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt
     6181 cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga
     6241 atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt
     6301 attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga
     6361 aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat
     6421 gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg
     6481 aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg
     6541 tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg
     6601 ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc
     6661 gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata
     6721 cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt
     6781 tatcctaaaa aagggatacc aaccatcaat atcgttccca caggaaaaag tggcccatta
     6841 gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca
     6901 ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac
     6961 aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca
     7021 aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca
     7081 gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt
     7141 gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga
     7201 aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg
     7261 cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg
     7321 aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat
     7381 gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa
     7441 gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt
     7501 gcttgggtag caagttttgg attatatagc tga
//
LOCUS       DAYYJC010000111         7159 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000111 DAYYJC010000000
VERSION     DAYYJC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.113"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="ACHA9N_002413"
     CDS             complement(1778..2443)
                     /locus_tag="ACHA9N_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085841.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2569..4605
                     /locus_tag="ACHA9N_002414"
     CDS             2569..4605
                     /locus_tag="ACHA9N_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM9085842.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="ACHA9N_002415"
     CDS             4609..5247
                     /locus_tag="ACHA9N_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9085843.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="ACHA9N_002416"
     CDS             5275..5703
                     /locus_tag="ACHA9N_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085844.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..7071
                     /locus_tag="ACHA9N_002417"
     CDS             6160..7071
                     /locus_tag="ACHA9N_002417"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9085845.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2465 a         1074 c         1314 g         2306 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     7081 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt ttattagaac
     7141 taaatcaaca gtagtttaa
//
LOCUS       DAYYJC010000112         6895 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000112 DAYYJC010000000
VERSION     DAYYJC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.114"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA9N_002418"
     CDS             complement(<1..182)
                     /locus_tag="ACHA9N_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9085846.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA9N_002419"
     CDS             517..951
                     /locus_tag="ACHA9N_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9085847.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA9N_002420"
     CDS             974..1294
                     /locus_tag="ACHA9N_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085848.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA9N_002421"
     CDS             1435..1626
                     /locus_tag="ACHA9N_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085849.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA9N_002422"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA9N_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9085850.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA9N_002423"
     CDS             2112..2375
                     /locus_tag="ACHA9N_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085851.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA9N_002424"
     CDS             2399..2587
                     /locus_tag="ACHA9N_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085852.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA9N_002425"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA9N_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085853.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA9N_002426"
     CDS             3070..3441
                     /locus_tag="ACHA9N_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9085854.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA9N_002427"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA9N_002427"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA9N_002428"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA9N_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085855.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA9N_002429"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA9N_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9085856.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHA9N_002430"
     CDS             5250..5447
                     /locus_tag="ACHA9N_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085857.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHA9N_002431"
     CDS             5553..6035
                     /locus_tag="ACHA9N_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085858.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHA9N_002432"
     CDS             complement(6093..6689)
                     /locus_tag="ACHA9N_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9085859.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2332 a         1060 c         1355 g         2148 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttg
//
LOCUS       DAYYJC010000113         6780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000113 DAYYJC010000000
VERSION     DAYYJC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.115"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1459
                     /locus_tag="ACHA9N_002433"
     CDS             41..1459
                     /locus_tag="ACHA9N_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9085860.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1530..2999
                     /locus_tag="ACHA9N_002434"
     CDS             1530..2999
                     /locus_tag="ACHA9N_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9085861.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3009..3476
                     /locus_tag="ACHA9N_002435"
     CDS             3009..3476
                     /locus_tag="ACHA9N_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9085862.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3512..4384
                     /gene="scrK"
                     /locus_tag="ACHA9N_002436"
     CDS             3512..4384
                     /gene="scrK"
                     /locus_tag="ACHA9N_002436"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9085863.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4465..>6780
                     /locus_tag="ACHA9N_002437"
     CDS             4465..>6780
                     /locus_tag="ACHA9N_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9085864.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFL"
BASE COUNT         2320 a          930 c         1350 g         2180 t
ORIGIN      
        1 ttagttgtga agataaaaac cttttcactg gaggaagaaa atggagaaat tattaattaa
       61 aagcaataat catcaacttt actaccaacc taaggatgtt tgggtcggag atgttatgcc
      121 ttacgggaaa gaaggaaaat tctttattta tcatcaacgt gatactagaa atccagttcc
      181 ttttggagaa ccgtttggct gggctttagc aactacgaaa gattttgttc attttgaaga
      241 ctttggggaa tctcttttaa gaggggatga caatgagaga gaccagttta tctacgctgg
      301 ttctgtattt gaggcaaatg gaaaggttca cgcattttat acagggtata atcgtgaatt
      361 cttagcgcag ggaaagacat ctcaagtgtt gcttcatgct gagagtgatg attttgttca
      421 ttgggaaaaa tcagaaacag cattggagtt agaaccacaa gaagggtatg atattcgaaa
      481 ctggagagat ccatttgtcg tttgggatga tgaaaaagaa gaatatttgc taattttagg
      541 ggcgcgaaag ggtcaggata aacatctaca aaccggacgt ttagtggaat ttacatcaaa
      601 agatttaact aattgggaat tcaaaggtga tttttgggct ccgggattgt ataccatgtt
      661 tgaaatgcca gaacttttca agattggtga ctggtggtat cttgtctatt cagaatactc
      721 agatggaaat aaaatactct atcgaatgag tcaaagtttg gaaggtcctt ggattaagcc
      781 taaagatgaa gcttttgatg gacgggctta ttatgcaggt agaacagctt atgatggaaa
      841 acgtcgagtc ctatttggat gggtaccaac aaaagatcaa gaaaatgata aaaacaatta
      901 tttatgggga ggtactttgg ttcctcatga agtctatcaa aaagaggatt tttcacttgg
      961 ggtaaaaccc atagacgaag tgtggaatgc attcataaat ttacatgata ttgccgatgt
     1021 agagatagaa acagcagaca caagagaaga agtaacgctt gtcaaaaata gtggaacttt
     1081 gtttaattta gaaacaacaa tttcatttga tgagaatctt agtgactttt cactgagatt
     1141 atataaaaac cttgagacag atgaatctta cgaatatcgc ttcaatttag atgaggggcg
     1201 tcttacattt gataaaaacc cttgttaccc ttggtttcaa tatatggata aaggacttat
     1261 tagacctata tctttaaagc ctaaccaatc ttataaacta aaagtgattg ttgatgacaa
     1321 tataatgaca ctttatattg atggaatcgc tcttaacgct cgtgtacatg gttgttttgg
     1381 tcaagcaatt actattacag caacaaatgg atcaatgact gcaataaata ccaagttttc
     1441 aaatgaaata aagaaataat aaacgtcaat catgtggcag gtctgaaaat caaacatgac
     1501 ctgtcacatt taaaagggag gataaatgaa tggactacaa aaaaattgcc gaagatgtat
     1561 tggaaaacgt tggaggaaaa gagaatatct caagcgctac acactgcatt actcgtttac
     1621 gtttaattct taaagacaaa agcaaagcga atatggaaat gcttgaggaa atagaaggtg
     1681 caaaaggtgt tcttttcaat agtggacagt tacaagtaat tttcggaaca ggtataattg
     1741 aaaaggttta caaagagttc gttgaattga caggagctaa ggaagtatcc gtttctgaaa
     1801 tgaaagacga aggagtcaat aattcaggaa atagacttca acgagctttt aaagttttct
     1861 cggatatctt tattccgatc attccagctt ttattggggc agcaatggtt ttaggaattc
     1921 gttcgttgat taccactcct ggaatgtttg gaatggatgg ctcattcgct gatcacaatg
     1981 tattcctaaa tgatttatcc aaatttctgg gtattattgc aaccacattt gattatttac
     2041 cagttttagt tatgtattct gcaacaaaac gttttggcgg aaaccctatt ttaggattac
     2101 ttgtaggatt tgttatgatt catccaggac tagccaatcg gaatgatttc gtcatgggaa
     2161 ctataaagcc tgattattgg catttatttg gattaagcat tccagcagtt gcgtttcaag
     2221 ggggtgtttt tccagcaatt ttgacttctt ggtttttagc aaaagttgag aaaattacaa
     2281 ataaatattc tcctcaagta cttgcttaca ttttagtccc aactgtgaca attgttcttg
     2341 ccaactttgc actattttta gtgtttggtc cattggggaa ttttatcggt aatgttttag
     2401 gtggaatcat tgatttccta tatatgaaga tgggtgcctt tggtgctttt gtttttgcag
     2461 gcgcgttaca accattagtt gtaacaggaa cacaacatgc tattcaaggt attgaggcaa
     2521 acttagtcgc aacaactgga ttcaattata tccaacctat ttggtcagtt tcaattattg
     2581 cccaaggtgg cggttgtatt ggtatgtatt tactctttaa gaaaaaatct aaagatagag
     2641 atattgcgat ttcaagcttt attccaacac tggtaggtat tactgaaccg gctatctttg
     2701 ctgttaattt gaaatattca atcgttcctt ttgtttgctc gtttattggt gctggttttg
     2761 gtggagcatt tatgaaaatt tttgatgtaa aagccattgg acaaggattg actgtattgc
     2821 caggtctgac tattgctaat ccagttcttc cttatatatt tggtaatctc atagcattca
     2881 ttttaccaat catttttatt ctcgcttatg ataaatataa agtgtttatc cctgaagcta
     2941 aacaccgtaa atttgcagaa aaaactgact caaaaaaatt gccagttcaa gtaaagtgag
     3001 atgagagcat gttaaatttt tttagaaaaa atgaaataag cgttgtagca cctgcaaccg
     3061 gtgtttttga aaaactggag aaattaagtg atccagtctt ttcaaaagga atgatgggac
     3121 aaggatttgc aattgatgca aaaaacgaaa cgattgtatc tccaatcgat ggttttgttt
     3181 caagtatttt tcctacaaaa catgccttag gaataaaaac aaaatcagga attgagatac
     3241 ttgttcatgt aggaatagat acagttgagc taaatggtga gggatttgat attaaagttc
     3301 aagaaggaca aaaagttaag gctggggata ttcttatgac cattaatttt gtagtttttg
     3361 aaaagaatca caagactaaa gatgtcattg ttgtttttcc agactaccaa gacgaaattt
     3421 tcattccgaa acttgaagaa gtaaaacaag gacaagaatt gtttgttctt ggctaatgat
     3481 aagtaattaa taatagaatc gaggaaaatg tatggttttt ggggctattg aagctggtgg
     3541 aacaaaattt gtttgtgcca ttggagataa agatttgaac atatttgaaa aagtcagctt
     3601 tcccacgact acacccaaag aaacaatgaa tttagtttta gattttttta atcatcataa
     3661 agatgattta gaaggtatcg gagtgggatc gttcggtcct attgatattc attgtgaatc
     3721 taaaacgtat ggcttcatta catcaacgcc aaaacttgct tggcaggatt ttgattttat
     3781 tggaagttta aaaaagcaac ttaatattcc aactgcttgg acgactgatg taaatgcctc
     3841 ttgctatggt gaatatgttt ctggaaatgg cagaggcctt tcaagtgttg tttactatac
     3901 cgtaggaacg ggcataggag gtggcgctat ccaaaacgga aacttcattg aaggttttag
     3961 tcatccagaa atgggacata tgcttgtaaa acgacacaat aaagacaact tttctgggac
     4021 atgtccattc cacggagact gtttagaggg cttagctgct ggtccggcaa tcgaaagaag
     4081 atcagggaaa aagggacaag aagtgactga aaatgacgtt acttgggaaa tagaagctta
     4141 ttacattgct cagtgtgcat ataatacaac attgatgtta tcacctgatg tcattatatt
     4201 tggtggcgga gtaatgaaac aaaaacactt aaaaaagaaa gtacaagata agtttattga
     4261 gttgatcaac ggatatgtcg ctattcccga tgttgacaat tatattatga caccaaaatt
     4321 gttagataat tctggtattg taggttgttt agctttagca agagacgtta aagaaaaatc
     4381 ttaatttgga aaaaagatcg tctcgactat ttgtttggtt gatgcgatct ttttttcaat
     4441 tagtaattga agggacgcct aaaagtgaaa aaaaaaatga aagatgaagt attagagtat
     4501 atcaaaaata atttaaagta ttacgatttt tctgctcaag atattgcaat gaaattttgt
     4561 attaaaagaa atgtcgcttc ccactatttg aatcagttat tttcagatgg aaaattacta
     4621 aagaatgatt ctgttagacc agtaatgttc aagtataatc aacaaaagcc aaaagactgc
     4681 ttttctaagt ttattggagc agacatatct ttgaaatcca ctatagataa atgcaaagct
     4741 actgtaatgt atccccctaa tggcctccca ttaataataa aaggaaacag tggcgttgga
     4801 aaaagttttt tagcaagttt gatctatcag tatgctcttg atagaaaagt aattcataat
     4861 gacgcaaaat ttgttgttgt gaattgtgca gattatgcaa ataatccaga gctattgtca
     4921 gcagttttat ttggatataa aaaaggagcc tttactgggg ctgaaaaaga tactgcaggg
     4981 atgctatctt ctgctaatgg aggatacctt tttttagatg aggttcacaa tctaagtgca
     5041 gaaaaccaag agaaattgtt tcttcttatg gattctcaaa agtatcgtaa acttggtgaa
     5101 agtgtgcaat gggagtatgc aaatgttcgc cttatcttag ccacaacaga agataaaaat
     5161 acctctttgt tagcaacttt tagaagacgc atcccagctg aaatcactct gccagattac
     5221 aataatagaa gtacatctga aaaaattcaa ttattatttg aatttcttaa agatgaagca
     5281 attacgattg attcaaaaat attttgttcg attgaacttt ttactgatct tttatcgaaa
     5341 acatttgaag gaaatgttgg agaattgagg aatgaaatca agcttctttg tgcagaaggt
     5401 tatttaggaa ataagagaaa gtcttcaatt tatttagggg acaaactaga cagcggtttt
     5461 tggatagatc cagaaattag tatagattta aaaaaaatgt ttcttaaatc actttctgaa
     5521 attgatttga ttgaactttt tcaaaatcac atttcaattg aagaaagcat gagtcaatta
     5581 agagatagaa tacttaaaga gtgtgcaggt tcttctgagt ataattattt agaaagtgac
     5641 gaatttatag cattaagaaa ctatgttgtt aataaaatat caccaattat tgattcgaca
     5701 ggtctatgtt tattagatga attcttggcg gatatcagtc tatttataat gtttattgat
     5761 gtaattgaag aaacatcaag aaattttttg ctgtctaagt tcagaaaatt tcgtatgaaa
     5821 aatgataaac aaaaattatt ggcaaatgag atttggagta gtttagaagt cgaagaaagt
     5881 aaacaagaac ttttgttgaa atggatactt tttcttgtaa agaagttcta tgtgaaaata
     5941 ccagaaaccc attgtctgat agttatgcac ggtaaaataa cagcttctgc gatcgcaagg
     6001 gaaacaaata agttgctgaa tacatatgtt tatgaggctt tcgatatgcc aattgaaggt
     6061 gagactagtg acttaattaa cctaatcaac aatttttgta aaagtataga tacgacgaat
     6121 ggactcattc tgctagttga tatgggatca ttggaacaaa tgtatgaaaa aattgaatcc
     6181 aatgtaatag gtgatttggt aattttaaac aatgtttcta cagctttagc gattgaatgt
     6241 gggattcaag tttgccaaaa aaagccaatt agtcattttt accagatgga ttttgattcg
     6301 tttcaagtta aagttcagta ttataaagga ttatcacaaa agaaaaatgt aatagtatct
     6361 tgtctctctg gtgaggggat ttctgaaaaa gttaaggata ttttaaaacg gtatctcgat
     6421 aatcaagtgg agattttgat ttttgatttt aacgcattaa agaaaatagc aaaggaaaaa
     6481 gatactatga tttttaagaa tacaatttgt gttctttcaa ccactgaatt ttacattcaa
     6541 ggaatcgatt gtttaaattt agaaaatcta attaatggga atcaaacctt agaaaaatta
     6601 aataagtata tggactcgga tagctgtgag ttctgcttaa atgaattagt taaactcttt
     6661 actattgaag gcgctagtac taagcttcgc tttttagatt ccaaaaaagt attcaatgag
     6721 atagaaaaag tactatattt gtatgaaaaa tattacgatg tcaaaatccc aagttttctg
//
LOCUS       DAYYJC010000114         6481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000114 DAYYJC010000000
VERSION     DAYYJC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.116"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..1144)
                     /locus_tag="ACHA9N_002438"
     CDS             complement(182..1144)
                     /locus_tag="ACHA9N_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9085865.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="ACHA9N_002439"
     CDS             complement(1146..2585)
                     /locus_tag="ACHA9N_002439"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9085866.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="ACHA9N_002440"
     CDS             2770..4716
                     /locus_tag="ACHA9N_002440"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9085867.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /gene="scrK"
                     /locus_tag="ACHA9N_002441"
     CDS             4980..5852
                     /gene="scrK"
                     /locus_tag="ACHA9N_002441"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9085868.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6148..>6481
                     /locus_tag="ACHA9N_002442"
     CDS             6148..>6481
                     /locus_tag="ACHA9N_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9085869.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT         2071 a         1128 c         1152 g         2130 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaatcg taatacccgc
     6121 taaagcatac aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt
     6181 gtaatgatag aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt
     6241 aaacgtggac gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca
     6301 cttgaatgct ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt
     6361 tttcctgctg aggaaaaaga atacattgca aaaagatcaa ctgaaggttg gaccccagac
     6421 gtcattattg gccgtgcaga gcgaaccttc tcttgttcag taagtaccct ctatcgccgg
     6481 t
//
LOCUS       DAYYJC010000115         6361 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000115 DAYYJC010000000
VERSION     DAYYJC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.118"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1197
                     /locus_tag="ACHA9N_002443"
     CDS             118..1197
                     /locus_tag="ACHA9N_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9085870.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1753..2127)
                     /locus_tag="ACHA9N_002444"
     CDS             complement(1753..2127)
                     /locus_tag="ACHA9N_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9085871.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(2150..3550)
                     /locus_tag="ACHA9N_002445"
     CDS             complement(2150..3550)
                     /locus_tag="ACHA9N_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFM9085872.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(3554..3964)
                     /locus_tag="ACHA9N_002446"
     CDS             complement(3554..3964)
                     /locus_tag="ACHA9N_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9085873.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(3982..4473)
                     /locus_tag="ACHA9N_002447"
     CDS             complement(3982..4473)
                     /locus_tag="ACHA9N_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9085874.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(4483..5274)
                     /locus_tag="ACHA9N_002448"
     CDS             complement(4483..5274)
                     /locus_tag="ACHA9N_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9085875.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(5267..6040)
                     /locus_tag="ACHA9N_002449"
     CDS             complement(5267..6040)
                     /locus_tag="ACHA9N_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9085876.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(6196..>6361)
                     /locus_tag="ACHA9N_002450"
     CDS             complement(6196..>6361)
                     /locus_tag="ACHA9N_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM9085877.1"
                     /translation="DGITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYEL
                     IYTIFEQTIF"
BASE COUNT         1963 a         1376 c         1030 g         1992 t
ORIGIN      
        1 aataaaaaaa gggcggattt tataattaac ttagccattt ggaaataaaa aaacgccacg
       61 tgaattccat gttatattga agttaccaca actctcaata gacaaggatg aattcacatg
      121 acgcaaaacc atgataacac agtttcacgc aagggaaaac acctaaccta ctcagaacgt
      181 tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc gtcaggttgc ggatgtcctg
      241 ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg ggaccgttac tcagcttaac
      301 cgccagaatc agaacgggaa ggtctacgac tattatcatg acgtctacga cgccgatgcc
      361 gggcaggcgg cctacgagaa gaaccgcttg aactgcggca gacggccctt atgggccgac
      421 acggacgcct tcattgaatg ggcggacgac atgatgctgt tggaaaaatg gtccccggac
      481 gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc cggccattat ccctgggacc
      541 accaccctct atcagtggat cgaccgtggc atcatgaaaa cgaagaatat ggacctgcta
      601 gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca accgcccgaa taaacgggtg
      661 ctagggaaat ccattgaaga gcgcccggag gcggttggta cccggaaaac cttcggccat
      721 tgggaagtcg acacggtcgt aggaaacaag accaagtcgg atgccgtgct gctgacgttg
      781 gtggaacggc agacacgctt cgaggtcatt ctgaagctgg ccgggaagga tgccgaatcg
      841 gtcaaccaag cggtccgaca gctgagagaa cgggctggtg cgtacttcgg ccagctgttc
      901 aagacggtta cctcggacaa cggctcggag ttcgcgggac tgcacgatgc cttgcaggat
      961 gtggcaaaca tctacttcag ccacccctat gcttcctggg agcgcgggac gagcgaaaac
     1021 caacacaagc tgattcgccg tttcatcccc aagggcagcc caatcggcaa cgtgagcgaa
     1081 aaccaaatct tgcggattca gcgttggatg aacgattacc cgcgaaagat tttgggctat
     1141 gcaacgccgc atgacacgtt cgtccaggca tttaggcagg aacggttgac ggcttgacac
     1201 gtgggccctt gacaacgagc ctccctcggc atggcttgtg tctggaaagc tggccggggc
     1261 cagccagtcg ggagactacc atgcctcagc tcattgtcaa gggcccactc gattagaaaa
     1321 attagacggt tggtggtaac gagcaaccgg gtcaccatgg gtggctaact taaacttgaa
     1381 atttacgaaa ataaaaaaag gcatacagac aataaactgt ctatacgcct cacagactct
     1441 attctataca tctaattttg gctgtcctgc aaaaaatatg tgttagcctc catcgaaaga
     1501 tcaaactttg gaaaacatca ttataacttt gcttggtaag agctttgcta aaacatgata
     1561 tgacttgtat acaaaatgtg gagtgtattc caataaatag gaaggattac aaaatttgaa
     1621 accaaataag taaaaaatac tgtcactttg aattaatttg aaatagtaaa ttctcattaa
     1681 actattgaaa tgctgatacc aaaaaatgaa gtacttttcg ttcataccaa cttagagcac
     1741 ttcgttatct atttatttct tcatttggct tatctctata atctcatgat ttggtccgta
     1801 gaaattaaag taacggattc cattttccca gaacggtaat tccataatat ttttatctaa
     1861 taattcatat cctaaatctt gaattgaatt gaatacctca tctatgtcat ccgtatctaa
     1921 tgctagatga tcaatggctc ctctacactc agtagtttta tcttcccata tttctattat
     1981 cgttccattc aactcgaaaa agccgacttt acttcccaca ttcatttctt cattaatcag
     2041 actaaaccct aaggattcat aaaagatttt tgaagctcca aaatcttttg ttggaattcc
     2101 aatatgttgt aaaccattta atttcactta catcatccct tcaattgttt taaaatccac
     2161 attcataatt tattgaatag tctagtttat ctaccttgat tgctccctcc gtaacagtca
     2221 tacttaatgc tgttccgact ttatcataca ttctagtact taaagcaaca ttatcaatat
     2281 ataaaacagc tatcgtatca tcaacaatta actttagttc atattctttg cctatttcca
     2341 aaaatagtgg tcgttgcagc cccttatcaa acatttgagg ccaaggatag ttcggagttt
     2401 ttccgaaagt aagctgatta ttaatcaatg aaatacgaaa ttcgtatcct tcttgagtat
     2461 cactatctac aaataatttt aaagcaaact cctttgttaa cgatgtaata gtcaaagttg
     2521 tttcaaatag aaaataatct tcgattttag gaacaattac tctttctttt tttccaaatt
     2581 ctttctctaa cttaaaactt gaaaatggag tgatttcttt aaaattctca attatttttt
     2641 caggcagttt aacaccaaga gttttgtctg tcttttgaat aatttgatgt ggaacaagtg
     2701 caccgcccca atcccagtta ttttgatcgt tctccccttc ttttgttgcc acccagccag
     2761 ttagaaaacg gccagtttca ttagttccga ctgttcttgc tgcataatag gcttttccgt
     2821 cgaaagcttc gtctactggt gatttccaat ctgaaaataa tcctgctccg actcgatatt
     2881 tagtcttttt atcttcacaa tactctgaaa aaagtaaata ccacgttcca tcaatttcaa
     2941 atatatcagg catttcaatc atgttaaatt cattattagc ccagtagtct cctttgaaat
     3001 tccaatcttt taaatcttcg gattcaaaat agaccaatct ccccgtcggc tttttcttac
     3061 ttgctggttt tctagctcct agtatcaaaa cgtattttcc atgatcttga tcaaaaattg
     3121 caaaaggatc gcgccaatca ttcttatcat atccttcttg aggaacaagt tttgctgctt
     3181 cgccatcctt tttccaatga attaaatctt tagaacttgc aatcattaac acttctgaag
     3241 tttttccatc caacttagct ttgcgattat gtccagtata caaagctaaa tactcattga
     3301 ttccttggac aatacttcca gcgtaaatgt attgatcaat gtcatcgttg ccaccacgat
     3361 gaattacttc cccgtattct tcaaatttca caaaatcagt ggttgtaact agtgaccacc
     3421 caaaaggatc agttaaaggt ttaggatttc tcgtatctcg ttgatgaaat agataaaaag
     3481 ttccatcttt ataaaaaggc atgcaatccc caaaccaaaa atctttaggc ttgtaaaaaa
     3541 ttttttgcat ttatcactca tcctcttcgt tagtatgtct ttgcatatct gcaagacacg
     3601 caattgtatt catatttctt agattttcga tgatatcatc gctttttttt ccttgtaatc
     3661 tcaaattgac agcctcaatc aaaattccta aattcatgcc accaagaagt tgaaacccat
     3721 tttgaaatac ttcacttctt ttagcaaatt cgttgaaagg tgttccacca atcaagtcag
     3781 caaataccat aacttcttga ttttttactt gagaaactat tttttcaatg ttttcacgga
     3841 attcttcaat gcctgcatct ccaagtgtta ccacatgaca atttaaatta tcgccaaaaa
     3901 acatttttaa tgtatctaca aatccaatgg caatatttcc atgagatgta attataaact
     3961 ccatccactc atcctctgtt cttaataaat aaaatccttt aaaaattctt ttgaatctct
     4021 aggagtttgt atcactgtca cttcaactcc atcactaatt agttctttaa aagcctctct
     4081 ctcttcttca gtaatattta tcgtcggacg gatgcgttct gttccattcc ttcttgataa
     4141 atttcctacg tttacacttg ttattggtaa accgttatca atcaaaaatt taacatcgat
     4201 tggagaaact gcaacaataa gaactttttg tccttcatat ttaccagaaa gaatattttt
     4261 aactgctact tcccttgtaa ttattgaagt tctaacatta ggtggtgcag cgaggcgaac
     4321 tacagctttt tttacgtcat cattggctac ttcattatta ataaccataa ttctgttagc
     4381 actcaattga tttgtccatt gtgttgctac ttgtccatga atcattcgat catcaatacg
     4441 aacatgtata atacctttca ttttttcatc catattcaac acctacttca aaatatttaa
     4501 cgctcttaac gcaatagaaa ctactaagat taatagaatt acataaactg gtttaacctt
     4561 tcgtcctaac aaataataca caattgctac tacacatgca ggtaataaac caggcataac
     4621 ttgatcaaag atttcatttc cggtcattgt taagtcgcct tgtttaaatt caaagccaac
     4681 attcactttt acaactgtgg caatgagtgc accgaccacc attaacccta aaattgatac
     4741 tgcatcagta atagacttta attttgtttc aatttgagtt agtaggctcg cacccgattc
     4801 tttcccagca aggtataaag gataagcagt aatccttaat aaaattgatg ccagtatcca
     4861 caaaataatt ccaacaaaac taccttccaa tgccatatta gctgcaattg ctccaaaaat
     4921 agtccatgga ataactacaa atacactatc tccaattccc gctaaaggac ccattagacc
     4981 tgttttcaga gcacttactg cttccaaaga cttatttttt tcttcttttt caattccaac
     5041 attcatacca ataatgtaag gcgctgtata cggcgtagtg ttaaaaaatt gtaagtgagc
     5101 taaaacggat tgtttcattc cttcaggatc atctttatat gtgtccttga taaaaggaag
     5161 catagaatac gcataaccca acccttccat tttttcataa tttaatgaag ttgcaccatt
     5221 gagaatccac cggaaaatta cttgtttgtc gatttttgtc agtttcttat tcatcttcgt
     5281 caaaacctcc tgttgcagct ggtgctactt gagtattatt tgatctattt ttatatacga
     5341 ttagtgcgat tgcagcgcca agtaacgaaa tccctaaaat tggtatttgg aaataaactg
     5401 ccaaaacgaa accaactaac aaataggcaa agtaatcttt tgtaggtaaa tatttaagta
     5461 gcattcctat cccaacagca ggtaatagtc cccctgctac ctttaaacca ccggtaaccc
     5521 attcaggaat ataattgatt gcacttccca ctaacgttgg gcctaatatc acagttaaca
     5581 aaacaggaat tccagataca aagcttgtat taattgatgc caaatattgc atccactcta
     5641 atttttgata atcttctttt tgtgcgaatt tttctgcctt ttgttgaaaa aaaatgtttg
     5701 ttgaccactt tagaacatct agttgtacaa ttaacattgc taccggtatt gctaaagtta
     5761 ttcctataga agcatcctgg ttagttgaga ttgctaaata agtaccgatc aatgctgctg
     5821 tttgataatc cggtactgat gcaccaccaa aactagtaat acctagtgtc attaattgca
     5881 aagtcccacc gatataaagt ccagtttgaa tatctcccat aataagtccg gtcaagaaac
     5941 ctgcaacgac aggttgggag gttccgattt tgggcccttc cttatcccag ttaatgaaaa
     6001 atccatacaa cacaactaaa aatgtttgcc aaaacgccat tttgttctct cctttcttct
     6061 tatactttta tataagcaat tactgtgcca actaacatta tccaataaag cgggaaaata
     6121 cagtaaatat taattaaatg agcgtctaaa tatactttgg acaccctttc atttttttag
     6181 acgctagtat gctatttaga aaatagtctg ctcaaaaatt gtatagatta actcatactc
     6241 tcctttgggt atttcaatac agtaaatatc tttgatttct gaaaaaaaat cttttgatac
     6301 atttaaaaaa gtatcaaata gttgcgctcc ctctttcaaa tgttcatttg ttattccatc
     6361 a
//
LOCUS       DAYYJC010000116         6287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000116 DAYYJC010000000
VERSION     DAYYJC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.119"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACHA9N_002451"
     CDS             190..690
                     /locus_tag="ACHA9N_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9085878.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="ACHA9N_002452"
     CDS             787..2634
                     /locus_tag="ACHA9N_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM9085879.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="ACHA9N_002453"
     CDS             2637..3533
                     /locus_tag="ACHA9N_002453"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9085880.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="ACHA9N_002454"
     CDS             3583..3972
                     /locus_tag="ACHA9N_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9085881.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="ACHA9N_002455"
     CDS             3959..>6287
                     /locus_tag="ACHA9N_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085882.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DAYYJC010000117         6259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000117 DAYYJC010000000
VERSION     DAYYJC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.120"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACHA9N_002456"
     CDS             <1..411
                     /locus_tag="ACHA9N_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFM9085883.1"
                     /translation="QANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQFTF
                     KPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIK
                     QLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            432..965
                     /locus_tag="ACHA9N_002457"
     CDS             432..965
                     /locus_tag="ACHA9N_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9085884.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            981..1151
                     /locus_tag="ACHA9N_002458"
     CDS             981..1151
                     /locus_tag="ACHA9N_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085885.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            1601..2134
                     /locus_tag="ACHA9N_002459"
     CDS             1601..2134
                     /locus_tag="ACHA9N_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085886.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2146..2349
                     /locus_tag="ACHA9N_002460"
     CDS             2146..2349
                     /locus_tag="ACHA9N_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085887.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            2671..3051
                     /locus_tag="ACHA9N_002461"
     CDS             2671..3051
                     /locus_tag="ACHA9N_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9085888.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3033..3947
                     /locus_tag="ACHA9N_002462"
     CDS             3033..3947
                     /locus_tag="ACHA9N_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9085889.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            4192..4881
                     /locus_tag="ACHA9N_002463"
     CDS             4192..4881
                     /locus_tag="ACHA9N_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085890.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(5002..5088)
                     /locus_tag="ACHA9N_002464"
     CDS             complement(5002..5088)
                     /locus_tag="ACHA9N_002464"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9085891.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            5764..>6259
                     /locus_tag="ACHA9N_002465"
     CDS             5764..>6259
                     /locus_tag="ACHA9N_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9085892.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKE"
BASE COUNT         2306 a         1096 c         1168 g         1689 t
ORIGIN      
        1 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
       61 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
      121 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
      181 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
      241 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
      301 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
      361 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
      421 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
      481 agatgagtag gcaagcaata tcgaaacata tccaaaaatt agattcttcc atgatagcta
      541 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
      601 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
      661 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
      721 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
      781 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
      841 aaaaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
      901 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactttgga
      961 aataactgga gggaacattc atgacaagaa aaaatcttct attcggttct attttactag
     1021 gaattctagg attcagtccg atgttattgt cagtgatcaa taaagggaat aaacatggtg
     1081 aattgaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa
     1141 cagataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt
     1201 attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata ttttattaaa
     1261 aataacaacc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaacc
     1321 gcagaaaatc aagcttaaaa agagcttttg acctagacca atcgaaaatc acaaagtgag
     1381 cgcctgtatc tattgctaca ggcgctactt ggcatagtac acattatgtc aacaagaatt
     1441 taatataaca tattttccta atgcattcta actataccac attatcaaaa atataaatta
     1501 tctttactaa ataaaatatt gacaagccta taccttttgt tttatgattt tcacaaagaa
     1561 aacgttttct atatattttt ctgtatcagg aggaaattct atgaaaaaat ttactttagg
     1621 ggtagctttg ttaactattg gggcagttgt tttaccgtca actactatag tttatgcttc
     1681 agaaaattcc caagtggtat cattaaacga aaacaatgct actgaaaatg tagttattga
     1741 gtacactgaa gatggtatgg tcgtaactaa atatgaggat ggttatagtg cggaagattt
     1801 tgcaaattct tcagatatca tgacaagagc tgcagttgca agatggggca aaaaggaata
     1861 tacaaatatt gctgtaacca ctggagtagc tgctaatgct attaatgctg ccttttgggc
     1921 aggagggaaa gctagcactt ctatgtttgg tatcccaggt tgggcaattg gtgctttgtt
     1981 aacaggtgca agttggacta aaaaaggtgc ttctccaggg aatgcagttg caaaagaatg
     2041 ggacaaaaat ggtaatggct ggattggatt ttattaccaa aggggatatg atgcagctgg
     2101 aagagttgta gctactagat attcaacgtt ataagttaag gagtgatgtt tttgatatct
     2161 aaatcattaa aatcattaaa aacatttact aaaaagaatc cgcaaatagc tactattcgt
     2221 atttttatat ttgctatagc tgttgctata attgttaata tgctaagcgg agggcaatta
     2281 tacaaatctg gtttattttt agtgttaatc ttagttgtct tagacataat aaagtctaaa
     2341 aaaaaataaa tcgatacatt ttagaagaat agggttgttt gaatattaca aagcaacaat
     2401 ttcaccttcg gtgaaataag cgaaacttta caaaaagtgg ctagcaaatg ctagcctttt
     2461 tttgtattgt ggagcgtgga ttttgagggg gattcaaggg ggagcttgcg ctcctttgac
     2521 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     2581 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     2641 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aacatcaaaa tttggctagc
     2701 gatcattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
     2761 gaatccgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
     2821 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     2881 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgccaag
     2941 tggtgcaata cacaccaaca cgacgttgct cctgatgatg ctcaacgttt atccgaaaac
     3001 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agtcgaacca
     3061 cttcatgtag ccgagcaatc aattacgcag aaccacgagc aaccgtcaaa acaggaataa
     3121 actgtgacat tgattatgcc aaaagtgaaa tgaaacaaat acgcacgctt tatggaaaag
     3181 acgatcgtgt acaagcacac ttattgattc agtcctttcg accaggagaa attaccgctg
     3241 aaaaagccaa tcaactaggg gcagagtatg cgaaaaaaat agcccctaac caccaaatcg
     3301 cgatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     3361 atcttgaaac tgggaaaaaa tttcaagccc atgggcaagc atttttaaac aaatgttatg
     3421 acctaaatga cgagatttgt cttgaacatg acttaacaat cacggaacga ggaaagaaaa
     3481 ccgaaaaacg aaccatgtct gaaattaaat taaaagaaaa aaatgaaccg gtttggaaag
     3541 acgaaattcg gtttgcgatt gatcaaacaa tgaaaaacaa aaaaactcga acgtataccc
     3601 aattttgtga ttcacttaaa acattcggca ttcattgctt gaatcgcgga aaaaatttta
     3661 cttacgaatt ggtccacaaa aagaaaaaag tacgatccaa caaactagga aaggattacg
     3721 aaaaggagac gattttacat gagctggaca gacgagaacg aacctacaat caacaacgaa
     3781 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     3841 aacaacgaac ttttacgcct gaacaatcgg aaattagacc gactcgaaga aaccatgaac
     3901 aacacgttga acgaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     3961 atcgaaaacg aaataaagag acaacgtgcc tttcttgcgc cttataccca agaaaggata
     4021 caaactacga tggattcaca agtcaaaaaa ctccaccaag cgctattaga ccagcgaaac
     4081 caagaacaaa aaaaggcttt aattcaacga tcgatggata ttcttgcccc cttatgttgc
     4141 ttagtactaa cattattagc cttttggttg gtatggggag aataaacaga catggcattc
     4201 tatttaccct atctactcat ttctgccagt attttaggaa ttatttggct tagctataag
     4261 gcttatcaaa caagatattc attaaaaggc tctaaaattc gatttaagcg atttttattg
     4321 gtcggggtta tatttgcctt atcaatcgtt ttaagtggtt tattcggcgt tttagagggt
     4381 aataaacgtg tttcaagaag cattttatta ggggatatca ctcaaaagta tgaagcagca
     4441 tgtgataaaa agaaaaaaga gcaagcatta gcacaaaaaa tggaggaatt taccgcttgt
     4501 tatgaggaca tgaacaatat ttttgtggat caagagaaac gtttaacaga taaaaacatg
     4561 aaaaaactca ctcgttttta tcaaaactta ccagaaaaac agcaaaaaga ggtacaagaa
     4621 aaatatgagc aagtaaaaaa agacgtgcaa tattttaaag atacgcagac agaagaagct
     4681 tgttgtaact tatttagcga tacaatccct ttttcaacca gtgaacaaga acgaaaagaa
     4741 cggcaacaat cggtcacata cgaacgttat gaaacactgc ttcagcaagc aacaaacata
     4801 caaaatccaa cgaaaaaaga aacggcatta aattatctag aaagtgtcaa ggaatggttg
     4861 gatcagcaac aacaaaattg aatttgatta gcaatcatgt taaactgaac ctgcaaatga
     4921 tcaaacatta gatacacgaa agccccagag aggtcggatt ctctggggct ttttttcagg
     4981 ttgatgtcgc ctttattgat tttacttctt gtttcggtta ttcaaccaat aactgaataa
     5041 agagacacaa acttctacaa gaagggggca aatgaaccgt tcaaacatta gatacacctc
     5101 cttccagtcg caagactgtc ggataggcga caagttatta taccttacaa aataaaatct
     5161 gtgtggtgtt tttaagtatc ccttattttc gggttgttag ggcttgccct gaccgcctgt
     5221 aaggcgcttt aagaacaaga taaggttttt tacccaagac tgtcttaaaa cgacttagac
     5281 gagctatcag aggcttcccc cacctccgtt actgacttgt ttttagcaaa ggagccttta
     5341 ggcgactgcg cagtacaatg tgagagcata gcgatcacac ttttttagcg accgtaggga
     5401 gcaaggtttt gaattggcag ttttgaaaaa tgcgcgaacc tgttggtaga aaaaagaagt
     5461 ggtttttagg cgggaaattt tgtcaccctc ttttataatc tctttttaaa ccttttttta
     5521 aacctcttta ccgtcggctg tgtccttact ccttcaatgg atacagcgtt ttataaacta
     5581 cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt gtctgctttt
     5641 aaactacatt ttgtctgctt ttaaactctt tttttgcact cacgtatgtt ataatatgca
     5701 ttatcacaga caaaacgttg tttataacgt ctgtatccat tgaaggagta aggaggaata
     5761 tctttggcaa atgaaatcgt gaaataccat catgaattaa ataccattcc gttgcggaaa
     5821 tttacaccag ttgaaatgaa tctatttttc tctattgtgt ctagaatgcg cgaaaaaggc
     5881 gataaaacag tccgtcttac ttttgaccaa ctaaaagatt taagtaatta taaagcaact
     5941 gctaataatc gtttcattaa tgatttagaa atgacttatg ataaattaat ggatttacgt
     6001 tttggtaaaa gaagtgctaa tgggctcgat atagatagat tcgttatgtt tagtgagttt
     6061 aaaatacgtg gaactgctaa agatccatat gtagacatac aagtttacga aaaagcttta
     6121 cctttactaa acaatttaga tgagtgggtt cgctattcac tccaacaatt taacgaatta
     6181 caaagtagct atgcaaagac tatgtttcga ttactaaaac aatatcgaac taaaggtttt
     6241 gcttattttt ctaaagagg
//
LOCUS       DAYYJC010000118         6229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000118 DAYYJC010000000
VERSION     DAYYJC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.121"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..293)
                     /gene="rpmB"
                     /locus_tag="ACHA9N_002466"
     CDS             complement(105..293)
                     /gene="rpmB"
                     /locus_tag="ACHA9N_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9085893.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            563..925
                     /locus_tag="ACHA9N_002467"
     CDS             563..925
                     /locus_tag="ACHA9N_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9085894.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            977..2659
                     /locus_tag="ACHA9N_002468"
     CDS             977..2659
                     /locus_tag="ACHA9N_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9085895.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2776..4812
                     /gene="recG"
                     /locus_tag="ACHA9N_002469"
     CDS             2776..4812
                     /gene="recG"
                     /locus_tag="ACHA9N_002469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9085896.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4861..5862
                     /gene="plsX"
                     /locus_tag="ACHA9N_002470"
     CDS             4861..5862
                     /gene="plsX"
                     /locus_tag="ACHA9N_002470"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9085897.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5984..6226
                     /gene="acpP"
                     /locus_tag="ACHA9N_002471"
     CDS             5984..6226
                     /gene="acpP"
                     /locus_tag="ACHA9N_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9085898.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2080 a         1085 c         1422 g         1642 t
ORIGIN      
        1 caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atatttatta
       61 aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact
      121 tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata
      181 acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa
      241 cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt
      301 tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc
      361 atatagaatg ggaatttgca agtacatttt catagataca attgacaatt gagaagaata
      421 attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa
      481 ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt
      541 tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga
      601 aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt
      661 tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga
      721 aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta
      781 tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt
      841 gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca
      901 aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg
      961 aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt
     1021 ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt
     1081 ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa
     1141 ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg
     1201 attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt
     1261 ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac
     1321 tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac
     1381 agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt
     1441 tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt
     1501 gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt
     1561 ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc
     1621 ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca
     1681 aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga
     1741 aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat
     1801 aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga
     1861 aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt
     1921 cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt
     1981 tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg
     2041 cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat
     2101 gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat
     2161 tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga
     2221 tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc
     2281 attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag
     2341 cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat
     2401 caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat
     2461 cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac
     2521 gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac
     2581 agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta
     2641 tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat
     2701 aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg
     2761 agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga
     2821 gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact
     2881 tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa
     2941 gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat
     3001 aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt
     3061 tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt
     3121 aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg
     3181 gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat
     3241 ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac
     3301 cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca
     3361 aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta
     3421 tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag
     3481 atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca
     3541 aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg
     3601 cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta
     3661 tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca
     3721 caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg
     3781 attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg
     3841 gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat
     3901 ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt
     3961 cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca
     4021 ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga
     4081 cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg
     4141 acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa
     4201 tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt
     4261 gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta
     4321 atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa
     4381 gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga
     4441 ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt
     4501 atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag
     4561 accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta
     4621 tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga
     4681 aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag
     4741 atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt
     4801 tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca
     4861 atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc
     4921 gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca
     4981 gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa
     5041 atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc
     5101 cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca
     5161 ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct
     5221 ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat
     5281 cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct
     5341 ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga
     5401 acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa
     5461 acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat
     5521 gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca
     5581 atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg
     5641 ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa
     5701 catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca
     5761 ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta
     5821 gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc
     5881 gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg
     5941 gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt
     6001 atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac
     6061 ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt
     6121 tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga
     6181 aacagtcggt ggcgcagtag attatatcag cagccatctt aaataattt
//
LOCUS       DAYYJC010000119         6227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000119 DAYYJC010000000
VERSION     DAYYJC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.122"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACHA9N_002472"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHA9N_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA9N_002473"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACHA9N_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9085899.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACHA9N_002474"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACHA9N_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACHA9N_002475"
     CDS             complement(2143..2373)
                     /locus_tag="ACHA9N_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9085900.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACHA9N_002476"
     CDS             complement(2603..3421)
                     /locus_tag="ACHA9N_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9085901.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACHA9N_002477"
     CDS             3582..4271
                     /locus_tag="ACHA9N_002477"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9085902.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACHA9N_002478"
     CDS             4285..5787
                     /locus_tag="ACHA9N_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9085903.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..>6227
                     /locus_tag="ACHA9N_002479"
     CDS             5800..>6227
                     /locus_tag="ACHA9N_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9085904.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLII"
BASE COUNT         2012 a          984 c         1134 g         2097 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taataga
//
LOCUS       DAYYJC010000120         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000120 DAYYJC010000000
VERSION     DAYYJC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.123"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA9N_002480"
     CDS             725..811
                     /locus_tag="ACHA9N_002480"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9085905.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHA9N_002481"
     CDS             complement(932..1621)
                     /locus_tag="ACHA9N_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085906.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHA9N_002482"
     CDS             complement(1628..1906)
                     /locus_tag="ACHA9N_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085907.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHA9N_002483"
     CDS             complement(1866..2780)
                     /locus_tag="ACHA9N_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9085908.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHA9N_002484"
     CDS             complement(2762..3142)
                     /locus_tag="ACHA9N_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9085909.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHA9N_002485"
     CDS             complement(3592..3879)
                     /locus_tag="ACHA9N_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9085910.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHA9N_002486"
     CDS             complement(3899..4123)
                     /locus_tag="ACHA9N_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9085911.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHA9N_002487"
     CDS             complement(4578..4748)
                     /locus_tag="ACHA9N_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085912.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHA9N_002488"
     CDS             complement(4763..5296)
                     /locus_tag="ACHA9N_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9085913.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHA9N_002489"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHA9N_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9085914.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYJC010000121         6002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000121 DAYYJC010000000
VERSION     DAYYJC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.124"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACHA9N_002490"
     CDS             <1..395
                     /locus_tag="ACHA9N_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9085915.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACHA9N_002491"
     CDS             398..1228
                     /locus_tag="ACHA9N_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFM9085916.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACHA9N_002492"
     CDS             1244..2095
                     /locus_tag="ACHA9N_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9085917.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACHA9N_002493"
     CDS             2092..2451
                     /locus_tag="ACHA9N_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9085918.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACHA9N_002494"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACHA9N_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACHA9N_002495"
     CDS             2624..2929
                     /locus_tag="ACHA9N_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085919.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACHA9N_002496"
     CDS             2929..3240
                     /locus_tag="ACHA9N_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9085920.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACHA9N_002497"
     CDS             3237..3410
                     /locus_tag="ACHA9N_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085921.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACHA9N_002498"
     CDS             3407..3823
                     /locus_tag="ACHA9N_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9085922.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACHA9N_002499"
     CDS             3823..4491
                     /locus_tag="ACHA9N_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFM9085923.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACHA9N_002500"
     CDS             4488..4865
                     /locus_tag="ACHA9N_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085924.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACHA9N_002501"
     CDS             4862..5161
                     /locus_tag="ACHA9N_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085925.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACHA9N_002502"
     CDS             5151..5309
                     /locus_tag="ACHA9N_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085926.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACHA9N_002503"
     CDS             5403..5819
                     /locus_tag="ACHA9N_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9085927.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAYYJC010000122         5908 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000122 DAYYJC010000000
VERSION     DAYYJC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.125"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..592)
                     /locus_tag="ACHA9N_002504"
     CDS             complement(134..592)
                     /locus_tag="ACHA9N_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9085928.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(605..1936)
                     /locus_tag="ACHA9N_002505"
     CDS             complement(605..1936)
                     /locus_tag="ACHA9N_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9085929.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1947..3389)
                     /locus_tag="ACHA9N_002506"
     CDS             complement(1947..3389)
                     /locus_tag="ACHA9N_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9085930.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3376..4140)
                     /locus_tag="ACHA9N_002507"
     CDS             complement(3376..4140)
                     /locus_tag="ACHA9N_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9085931.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4244..5119)
                     /locus_tag="ACHA9N_002508"
     CDS             complement(4244..5119)
                     /locus_tag="ACHA9N_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9085932.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5374..5458)
                     /locus_tag="ACHA9N_002509"
     tRNA            complement(5374..5458)
                     /locus_tag="ACHA9N_002509"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5422..5424),aa:Leu,seq:caa)
     gene            complement(5476..5547)
                     /locus_tag="ACHA9N_002510"
     tRNA            complement(5476..5547)
                     /locus_tag="ACHA9N_002510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5513..5515),aa:Gln,seq:ttg)
     gene            complement(5557..5629)
                     /locus_tag="ACHA9N_002511"
     tRNA            complement(5557..5629)
                     /locus_tag="ACHA9N_002511"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5594..5596),aa:His,seq:gtg)
     gene            complement(5691..5775)
                     /locus_tag="ACHA9N_002512"
     tRNA            complement(5691..5775)
                     /locus_tag="ACHA9N_002512"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5739..5741),aa:Leu,seq:caa)
     gene            complement(5796..5866)
                     /locus_tag="ACHA9N_002513"
     tRNA            complement(5796..5866)
                     /locus_tag="ACHA9N_002513"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5832..5834),aa:Cys,seq:gca)
BASE COUNT         1810 a         1256 c          900 g         1942 t
ORIGIN      
        1 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
       61 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
      121 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
      181 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
      241 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
      301 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
      361 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
      421 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
      481 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
      541 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
      601 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
      661 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
      721 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
      781 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
      841 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
      901 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
      961 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
     1021 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
     1081 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
     1141 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
     1201 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
     1261 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
     1321 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
     1381 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
     1441 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
     1501 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
     1561 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
     1621 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
     1681 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
     1741 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
     1801 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
     1861 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
     1921 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
     1981 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
     2041 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
     2101 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
     2161 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
     2221 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
     2281 ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt
     2341 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
     2401 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
     2461 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
     2521 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
     2581 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
     2641 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
     2701 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
     2761 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
     2821 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
     2881 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
     2941 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
     3001 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
     3061 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
     3121 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
     3181 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
     3241 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
     3301 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
     3361 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
     3421 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
     3481 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
     3541 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
     3601 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
     3661 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
     3721 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
     3781 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
     3841 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
     3901 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
     3961 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
     4021 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
     4081 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
     4141 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
     4201 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
     4261 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
     4321 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
     4381 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
     4441 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
     4501 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
     4561 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
     4621 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
     4681 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
     4741 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
     4801 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
     4861 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
     4921 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
     4981 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
     5041 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
     5101 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
     5161 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
     5221 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
     5281 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
     5341 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
     5401 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
     5461 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
     5521 cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca
     5581 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
     5641 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
     5701 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
     5761 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
     5821 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
     5881 aatctataaa ataaagaata aattatag
//
LOCUS       DAYYJC010000123         5809 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000123 DAYYJC010000000
VERSION     DAYYJC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.126"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACHA9N_002514"
     CDS             450..1604
                     /locus_tag="ACHA9N_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM9085933.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACHA9N_002515"
     CDS             1577..2020
                     /locus_tag="ACHA9N_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085934.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACHA9N_002516"
     CDS             3143..4075
                     /locus_tag="ACHA9N_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9085935.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACHA9N_002517"
     CDS             4092..4607
                     /locus_tag="ACHA9N_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9085936.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACHA9N_002518"
     CDS             4903..5184
                     /locus_tag="ACHA9N_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085937.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACHA9N_002519"
     CDS             5186..5602
                     /locus_tag="ACHA9N_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085938.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAYYJC010000124         5802 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000124 DAYYJC010000000
VERSION     DAYYJC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.127"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..2211)
                     /gene="copB"
                     /locus_tag="ACHA9N_002520"
     CDS             complement(40..2211)
                     /gene="copB"
                     /locus_tag="ACHA9N_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9085939.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2231..4417)
                     /locus_tag="ACHA9N_002521"
     CDS             complement(2231..4417)
                     /locus_tag="ACHA9N_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9085940.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4417..4626)
                     /gene="copZ"
                     /locus_tag="ACHA9N_002522"
     CDS             complement(4417..4626)
                     /gene="copZ"
                     /locus_tag="ACHA9N_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9085941.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4639..5079)
                     /locus_tag="ACHA9N_002523"
     CDS             complement(4639..5079)
                     /locus_tag="ACHA9N_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9085942.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5153..5692)
                     /locus_tag="ACHA9N_002524"
     CDS             complement(5153..5692)
                     /locus_tag="ACHA9N_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9085943.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1499 a         1304 c         1014 g         1985 t
ORIGIN      
        1 aggttgtgac acgttttttg tcacaacctt ttttcattat tatctgattt tcaatgtcaa
       61 agcatttaga gctacaacta ccgtacttaa cgacatcaag acagcaccaa ctgctggact
      121 taatatgata ccaattggcg caagaattcc ggctgctagc ggaattgcaa gaatattgta
      181 acctgccccc caccagaggt tctggatcat tttctttcgc gtttgcttgg ctagatcaag
      241 aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg acatccgcag aatcgattgc
      301 gatatctgtc cctgcaccga ttgccatccc gattgaagca cgggctaaac ttggcgcatc
      361 attgatccca tcacccacca tgattacttt tttcccttgg tcaaggtatt tctggacaac
      421 agcttcttta tcatctggta ataaaccgcc ataatattct tcgatcccta aataatctgc
      481 tactgcttgg gcagcgtttt tattgtcacc agtaagcatg acaggagtga tttttctttc
      541 tttcaatgtt cggataaatt cttttgcttc tgtttttact acgtcaccta aagcaacgat
      601 tgcagctaga tgattttcta aaatcaggaa gctgatggta tttccttgtt cttggtaagg
      661 tttcaataat gcttgttcta cgtttaatcc gagacgctca acttcttttt cattaactat
      721 tttcacttgt tgttttttga ctgttgcctc tagcccgact cctgaaagat tttttagatt
      781 ttcagcttga taaggcttga tttcatgttc tttcaaataa ttcataatgc caacagccaa
      841 tggatgattg gcattcgctt ccaaggctcc tagatattga aggatttcat ttttattgaa
      901 cgcatcatca agtacttcga cacccgttac agtaaatttt ccttctgtta gtgttcctgt
      961 tttgtccagc atgatcacat ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa
     1021 aagtaatccg tttttagctg ctattgatgt ggaacgagca acaactaatg ggatagctaa
     1081 acctagagca tgaggacagg cgatgataaa gactgttacc atacgttcta atgcattagg
     1141 aagatctgtc aagaacaacc aggcgatgaa tgccaggata cccacaatta atgcgacata
     1201 aaacagccat tttgccactt tgtcagataa tgactctagc ttggattttt ctccttgtgc
     1261 ttttcgcacc atttccataa ccttcgctag ataaccattt tcaccagttc cagttacttc
     1321 gatttcgatc gttccatccc cattgataga accgccaatc actttgtcat tggcttgttt
     1381 tttgattcct cttgattcgc cagtcacagc tgattcgtca acaattgttt cacctttcaa
     1441 gattacacca tctgttggca ttttatcacc agaccggacg atcaaacgat caccttcgtt
     1501 tacttctttc aatgaaactg tctcttcttt tccatctttc gtcaagcgtt tcactgattc
     1561 tggcagtaat tctgctagtt tttgtaaagc attgctggca ttcgacacag cattcatttc
     1621 aatccagtga cctagtagca taataacgat caaagtagcc aattcccaga agaaatccat
     1681 gacgtgtgta tgtggattga gcagatttgc gataaacgaa tagacactat aaatatatgc
     1741 aactgtaatc cccatcgcga tcagtgtcat catagcagga cttttttgtt tcaattccat
     1801 cttagctcca cttaaaaatg gctgaccgcc atagataaat agtatagttg ctaagatcaa
     1861 tacgagccaa tcagaaccag gaaacgttac ttgaaatgga aattccattc ccatcattgg
     1921 tgaaaaaaga atgattggga tagctaggat aagcgatagc caaaattttt gtttgaaatt
     1981 ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc
     2041 catttgtttg ctgtttgcta tatctccatg gttatgtccc atgtgcatgg aatgatccat
     2101 ctgactatgg tcatgtttca tctccatctg tttttcatga gattctgtac tttctttgtt
     2161 tactacttct tttttatgct catgtttttt ctccatatcg ttcccattca tttttactcc
     2221 atcccttctc ttactttttc atgtgtcgat ttaagcacag ggagttcaac aatacactga
     2281 cggaactaaa cgccatcgct cctcctgcaa tgattggatt caaaaagcct agtgcggcaa
     2341 aaggaatacc aatcgtatta tagacaaaag cccagaaaag attttgtttg atttttctca
     2401 atgtcaacct agacaactcg attgtttgtg caatggatgc caaactgcta ttcatcagcg
     2461 tcacatcggc tgtttccatc gcaatgtccg ttccacttcc cattgccata ccgacatcag
     2521 ctaatgctaa ggccggtgca tcattgattc catcgcctgc cataccgact tttttgccat
     2581 ctttctgaag tttttctacg taggctgctt tatcctctgg aagtacttca gcaaagatat
     2641 gctttggatc aataccaact tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg
     2701 tcagcataaa aacatctaat cctttattct ggagctgctt gattgcttgt ttgacttcta
     2761 gtttgatttg atcagccacg gcaatcaacc cgatcacttg cttttcatca gctaaaaaca
     2821 tgactgtttt tccctgctgt tctaattcta acgcgtgttc ttggtattca tcaaatgata
     2881 ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg ttaatcgtac
     2941 cgctgattcc agcacctggg tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt
     3001 gcagttttcc gtattccacg attgctttac ctagtggatg ttcagaagct tgttctaaag
     3061 tataaaaaat agataggact tctttagaac cgactagatc tgtaactttg ggctttcctt
     3121 ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg
     3181 cttctccgcc tttaattaaa attccatttc gtgcaccaac acccgttcct accataatag
     3241 ctgttggagt cgctaaacct aatgcacatg gacacgcaat gactaataca gagacactat
     3301 ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt agctatgagc gtgataaacg
     3361 cgatgactaa tacgatcgga acaaaaatcc ctgagatccg atctgcgatt ttttggattg
     3421 gggctttgct cccttgagca tcttccacca tttgaatgat ttgcgctaat actgtctgct
     3481 tgccgacttg tgtaacttcc atgtgaagta gaccattcgt attgattgtc ccgccaaaga
     3541 gcgtgtcatc cggattcttt tctacaggaa gattttcacc agtaagcata ctttcatcta
     3601 tcgcactgct tcctgaaata attcgaccat ctgctggaat ctgctcacca ggacggatga
     3661 ctagttgatc accaacgact acttcttcaa tcgctaacgt ttcttctttc ccgtttctta
     3721 tcacttgggc ggtttttgtt tgaagagaca tcaattgttt gattgcatta cctgttttcg
     3781 tttttgctgt atgttccaaa tatttgccga gcaaaatcag cgtgatgatc atactgctgc
     3841 tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa
     3901 aggcagccga tgttccaatt gctaccaata catccatatt tggtgctttc gtcttgattg
     3961 catggtacgc acctttataa aaacgagcac cgacataaaa ttggacagga gcagacagca
     4021 ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca
     4081 tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt actcaatata agatcgaata
     4141 gcattttttt caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt
     4201 acaaaattgc accatagccg atgttttcta cgctttggat taatttttca gtggtcgtcc
     4261 cttcatattt gacacttgct ttttcagtgg ccaagttgac tgtggcattg accacaccag
     4321 gttgtccgtt cagctctttt tcgatcctag cagaacaatt tgcacaagtc atacctgtaa
     4381 tgacgaacgt atccattttg gtttctgctg ttgccatcaa atcacctctg ctttataacc
     4441 caatccattg attgcttgac aaatcttttc ggattgaact tttgtttcat caaacttgac
     4501 cgtaccgttt gcttttttca aatttacttt cactttttgc acgccgtcaa gtgcgcttac
     4561 tgtttcttct actcttgctg cacaatgatt gcagctcata ccttcgattg aaaattgttg
     4621 tttcattttt tcttcctcct attgatgttg tttacattca cattgacctg ggatacagtt
     4681 acattcgatc gtatctactg gttgcttttg ttccagttgt ttcaaaatct cattgatatc
     4741 ttcttgagtc aaagtggctt cttctactag atccgcaatc gtctctccaa tcctttttgc
     4801 acagatatgg gaaaaaagat tttccgtcgc actgcgaact gtttccatct ccgagacagc
     4861 tggataataa atgaattttt ttccttcttg ttctgtccag atcacttctt tttttaccaa
     4921 acgacccagc aaagttttga tcgttgctgg tttccaatcc atcgaatcag ctaagatctg
     4981 cgtgatttct tgggctgtcg tctgtccaag tgtccaaata acacgcatga tctcccactc
     5041 tgaatcactg atctttactg gcgttttgac tttttccatt tatttcacac ctttcgttta
     5101 caaatgtaat cattatctta tgaatacaga ttacacttgt aaacaaaaac tgtcaattat
     5161 ttttgctctc tattttcaat ttagttaaaa attcttgttt ctctcgtctt gttttaagga
     5221 tgatcagttt atttcttgca ttagctactt ggatcagctg ctgtattttc ggttcattgt
     5281 tttcttcaaa atgccagata aaagataaaa attctaagta ttccctgttt agtttctccg
     5341 aaaatgattc aggcatatct ggccgagatt ttttgtttat ctttcttttt agagaacgcc
     5401 aaactactcg gtagatggcg agataacgcg gcactttaag ccaaatgatc ttatctgctt
     5461 ctttaatcct ttcctctaat gtagaagtat aatttccttc aacgatccat gacggattag
     5521 ataccataaa caattgctgt tcttgcaaaa accacttttt tgcctgaata ctgtagtctg
     5581 ttgtatgcca taaaaggtct agcggcagta gcggaaagtc ggtgatttct ttgatcttcc
     5641 ttgcgagggt cgattttccc gacccacttt gtccaatcac gatgatcttc attcttctcc
     5701 ctcacttctc tttggaattt ggttcgatca taccacttat aacgccgcaa aaaaacaagg
     5761 gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aa
//
LOCUS       DAYYJC010000125         5790 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000125 DAYYJC010000000
VERSION     DAYYJC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.128"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA9N_002525"
     CDS             complement(99..1229)
                     /locus_tag="ACHA9N_002525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFM9085944.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACHA9N_002526"
     CDS             complement(1877..3079)
                     /locus_tag="ACHA9N_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9085945.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACHA9N_002527"
     CDS             complement(3139..3342)
                     /locus_tag="ACHA9N_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9085946.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACHA9N_002528"
     CDS             complement(3484..4551)
                     /locus_tag="ACHA9N_002528"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9085947.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACHA9N_002529"
     CDS             complement(4609..4716)
                     /locus_tag="ACHA9N_002529"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085948.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACHA9N_002530"
     CDS             4879..5691
                     /locus_tag="ACHA9N_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9085949.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAYYJC010000126         5611 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000126 DAYYJC010000000
VERSION     DAYYJC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.130"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..2008)
                     /locus_tag="ACHA9N_002531"
     CDS             complement(59..2008)
                     /locus_tag="ACHA9N_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9085950.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="ACHA9N_002532"
     CDS             complement(2050..3108)
                     /locus_tag="ACHA9N_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFM9085951.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="ACHA9N_002533"
     CDS             complement(3227..3682)
                     /locus_tag="ACHA9N_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085952.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="ACHA9N_002534"
     CDS             complement(4266..5069)
                     /locus_tag="ACHA9N_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9085953.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2073 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat a
//
LOCUS       DAYYJC010000127         5596 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000127 DAYYJC010000000
VERSION     DAYYJC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.131"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..863
                     /locus_tag="ACHA9N_002535"
     CDS             6..863
                     /locus_tag="ACHA9N_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9085954.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ACHA9N_002536"
     CDS             885..1394
                     /locus_tag="ACHA9N_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9085955.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ACHA9N_002537"
     CDS             1431..1832
                     /locus_tag="ACHA9N_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085956.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ACHA9N_002538"
     CDS             1944..2297
                     /locus_tag="ACHA9N_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085957.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ACHA9N_002539"
     CDS             2331..2774
                     /locus_tag="ACHA9N_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9085958.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ACHA9N_002540"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ACHA9N_002540"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9085959.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ACHA9N_002541"
     CDS             3869..4582
                     /locus_tag="ACHA9N_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9085960.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ACHA9N_002542"
     CDS             complement(4789..4938)
                     /locus_tag="ACHA9N_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085961.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="ACHA9N_002543"
     CDS             5334..5534
                     /locus_tag="ACHA9N_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9085962.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1941 a          857 c         1216 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaattc
//
LOCUS       DAYYJC010000128         5539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000128 DAYYJC010000000
VERSION     DAYYJC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.132"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..356)
                     /locus_tag="ACHA9N_002544"
     CDS             complement(<1..356)
                     /locus_tag="ACHA9N_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9085963.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(654..1628)
                     /gene="sgrA"
                     /locus_tag="ACHA9N_002545"
     CDS             complement(654..1628)
                     /gene="sgrA"
                     /locus_tag="ACHA9N_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9085964.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1824..2663)
                     /locus_tag="ACHA9N_002546"
     CDS             complement(1824..2663)
                     /locus_tag="ACHA9N_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9085965.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2848..3735
                     /locus_tag="ACHA9N_002547"
     CDS             2848..3735
                     /locus_tag="ACHA9N_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9085966.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4319..5014)
                     /locus_tag="ACHA9N_002548"
     CDS             complement(4319..5014)
                     /locus_tag="ACHA9N_002548"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFM9085967.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4998..5396)
                     /locus_tag="ACHA9N_002549"
     CDS             complement(4998..5396)
                     /locus_tag="ACHA9N_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9085968.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1767 a         1104 c          812 g         1856 t
ORIGIN      
        1 tatataattt tttgactaag attttctaag gattgataag tacctttgta aagtgaatcc
       61 agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac
      121 tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct
      181 ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg
      241 tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa
      301 tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta
      361 catactcctt tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa
      421 tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc
      481 ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac
      541 aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata
      601 ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc
      661 actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc
      721 atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta
      781 ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt
      841 tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata
      901 ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct
      961 gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact ctccgtactg
     1021 ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt actgctttcg
     1081 ctgctcgtgt tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc
     1141 gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact
     1201 gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc
     1261 aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt
     1321 gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt
     1381 tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc
     1441 acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc
     1501 cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg
     1561 attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt
     1621 ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga attatataaa
     1681 atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt
     1741 cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat
     1801 atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt
     1861 gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat
     1921 atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga
     1981 gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt
     2041 gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata
     2101 agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta
     2161 ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga
     2221 ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc
     2281 actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct
     2341 aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca
     2401 acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct
     2461 tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa
     2521 tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca
     2581 agtgcattat tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca
     2641 atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt
     2701 ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt
     2761 acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc
     2821 tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt
     2881 atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg
     2941 attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt
     3001 aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta
     3061 tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa
     3121 ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag
     3181 aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca
     3241 gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt
     3301 ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat
     3361 ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa
     3421 atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa
     3481 ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa
     3541 agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac
     3601 aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat
     3661 aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa
     3721 aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat
     3781 ttttggaaat tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc
     3841 tttttttatt tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta
     3901 aaaaagtttt ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct
     3961 tccctatttt ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac
     4021 tgaaaagtta ttttaaaaaa gactaaaaat atcccttctg gacttgttcc tcttattctg
     4081 ttatactaga gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa
     4141 tttattcaaa actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca
     4201 tcgtttaaat gagaaataag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     4261 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     4321 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     4381 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     4441 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
     4501 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
     4561 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
     4621 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
     4681 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
     4741 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
     4801 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
     4861 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
     4921 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
     4981 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
     5041 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
     5101 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
     5161 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
     5221 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
     5281 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
     5341 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
     5401 gttcctcctc gtaaaatatc atttgtctaa tagtttttat tcaaaatgtt tctagattat
     5461 aaaatttatt gaataacaca ttcctaacgg aatttgtttc gatattgtgt gatttctcag
     5521 agtacaatgg atgtatgga
//
LOCUS       DAYYJC010000129         5531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000129 DAYYJC010000000
VERSION     DAYYJC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.133"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..985)
                     /locus_tag="ACHA9N_002550"
     CDS             complement(<1..985)
                     /locus_tag="ACHA9N_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFM9085969.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA9N_002551"
     CDS             1137..1832
                     /gene="vanR-A"
                     /locus_tag="ACHA9N_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM9085970.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="ACHA9N_002552"
     CDS             1810..2964
                     /locus_tag="ACHA9N_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM9085971.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="ACHA9N_002553"
     CDS             3179..4147
                     /locus_tag="ACHA9N_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM9085972.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA9N_002554"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="ACHA9N_002554"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM9085973.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="ACHA9N_002555"
     CDS             5177..>5531
                     /locus_tag="ACHA9N_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFM9085974.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DAYYJC010000130         5380 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000130 DAYYJC010000000
VERSION     DAYYJC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.134"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            629..1540
                     /locus_tag="ACHA9N_002556"
     CDS             629..1540
                     /locus_tag="ACHA9N_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFM9085975.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2027..2293
                     /locus_tag="ACHA9N_002557"
     CDS             2027..2293
                     /locus_tag="ACHA9N_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085976.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2365..2706
                     /locus_tag="ACHA9N_002558"
     CDS             2365..2706
                     /locus_tag="ACHA9N_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085977.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2761..3180
                     /gene="ssb"
                     /locus_tag="ACHA9N_002559"
     CDS             2761..3180
                     /gene="ssb"
                     /locus_tag="ACHA9N_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9085978.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3257..3553
                     /locus_tag="ACHA9N_002560"
     CDS             3257..3553
                     /locus_tag="ACHA9N_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085979.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3568..3999
                     /locus_tag="ACHA9N_002561"
     CDS             3568..3999
                     /locus_tag="ACHA9N_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9085980.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4304..4486
                     /locus_tag="ACHA9N_002562"
     CDS             4304..4486
                     /locus_tag="ACHA9N_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085981.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
     gene            4890..5378
                     /locus_tag="ACHA9N_002563"
     CDS             4890..5378
                     /locus_tag="ACHA9N_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9085982.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT         2007 a          791 c         1117 g         1465 t
ORIGIN      
        1 agggctgtcg gcgcttggta tgaaaggcat tctccgactt ccaggttttt gaccattttc
       61 tctcaacgtg gaaaaaccgc cacttatgag agaaaatggt caaaaaccta agcgggaagt
      121 ctccgaataa cataccaagc gccgacagcc aacagataaa gaatacacta ctaaaaatag
      181 tagtagcata aaatttaaaa gggaaaaaat caacccctac cccagtcttg gggtctcaaa
      241 actaactccc ccctaacccc ctctttcagc ctcttttgaa agagggggag aatcaacccc
      301 acggccaacg ccccagtgtt cttggggctg tccagaacag aaacttgcga gagagtgggg
      361 gtaaaacaag tgtagaggaa acttgaagac ttgttcgaac agaaacttgc gagagagccg
      421 gagtaaaaca agtgtagagg aaacttgaag acttgttcga acagaaactt gcgagagagt
      481 tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga
      541 ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa
      601 agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga
      661 ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt
      721 ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg
      781 agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg
      841 aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac
      901 gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat
      961 taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg
     1021 taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc
     1081 agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat
     1141 tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg
     1201 tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca
     1261 agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag
     1321 tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt
     1381 aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat
     1441 ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga
     1501 ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct
     1561 ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt
     1621 taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc
     1681 taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg
     1741 gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc
     1801 cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact
     1861 gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg
     1921 ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc
     1981 gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa
     2041 cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg
     2101 gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc
     2161 tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa
     2221 tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga
     2281 aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa
     2341 tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt
     2401 ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta
     2461 acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc
     2521 atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact
     2581 aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt
     2641 tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat
     2701 tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc
     2761 atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca
     2821 ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca
     2881 aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact
     2941 ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg
     3001 agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc
     3061 tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa
     3121 aataattctt caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa
     3181 gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga
     3241 gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt
     3301 gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta
     3361 tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa
     3421 ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc
     3481 gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga
     3541 aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg
     3601 ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc
     3661 ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa
     3721 gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat
     3781 ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca
     3841 cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact
     3901 ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt
     3961 ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc
     4021 ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga
     4081 atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc
     4141 taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg
     4201 acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta
     4261 cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc
     4321 gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag
     4381 agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac
     4441 agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag
     4501 aataaaaaaa tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata
     4561 catttttaat ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt
     4621 aaaaatgaaa aaatggaagc ggttacttat tcttttgaat ctaagataaa aattcaaaag
     4681 aataacataa cccaaatatg attatacttg agattctaaa tatttatcga aaaggaattt
     4741 gttttaagga aaaatgattc tatggacggt tattgattta tatttaccaa atggctaata
     4801 aaatagtatt tcttatattg tatagttttt gtttttatgt gaaaacatga gcaatagaat
     4861 tgtactagta ggataaggat ggatggaaaa tgaaaaaaac tatttttgtt ttagcgattt
     4921 ttatatgttt tgggctactt ttggtaggct acttgttgtt taattctaat gaaatgagaa
     4981 ctatttctac gaatgacttg ttaaaaaaaa gtgaattaaa ggataaaaaa aaagaatctc
     5041 ttgcctttat aatgtttact caagaagagt gtgagtattg tatagcaata aatccaatca
     5101 tagatagtgt aaataaggaa actttgaata agataaagaa atatgatata tctgtagatt
     5161 ttcaatctaa acaaaagtgt aaaattaagt ttgttccgac tattgttgca tatagtaaga
     5221 ataaagaagt ggggagaatt gacaatgaat tagataattt gtatcgaaaa aagcaagatg
     5281 gttcaattgt aatagacaag aaaaaaacaa agacattgct tatagattgg atgaaaaaaa
     5341 tggataaaca aggagaatat ttgtatggag aagattaaac
//
LOCUS       DAYYJC010000131         5259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000131 DAYYJC010000000
VERSION     DAYYJC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.135"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..950)
                     /locus_tag="ACHA9N_002564"
     CDS             complement(<1..950)
                     /locus_tag="ACHA9N_002564"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9085983.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFV"
     gene            1328..2719
                     /locus_tag="ACHA9N_002565"
     CDS             1328..2719
                     /locus_tag="ACHA9N_002565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9085984.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2735..3775
                     /locus_tag="ACHA9N_002566"
     CDS             2735..3775
                     /locus_tag="ACHA9N_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9085985.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            3794..4879
                     /locus_tag="ACHA9N_002567"
     CDS             3794..4879
                     /locus_tag="ACHA9N_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9085986.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            4912..>5259
                     /locus_tag="ACHA9N_002568"
     CDS             4912..>5259
                     /locus_tag="ACHA9N_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9085987.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSV"
BASE COUNT         1826 a          787 c          928 g         1718 t
ORIGIN      
        1 acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga gataaccaga
       61 ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga atatatttcg
      121 ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa gtcttctaaa
      181 gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat ttgttttcct
      241 ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact tgccgataaa
      301 cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac attggtttta
      361 ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata ataattgaca
      421 tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac agttgttagc
      481 ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg atagttgaat
      541 tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt ccaatcttca
      601 cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt cattgttagc
      661 aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt atactctgga
      721 aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa agcaatgcca
      781 atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc gtctttttta
      841 taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac aatttgacat
      901 atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat aagatattac
      961 ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt tcgattcttc
     1021 acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat tctctgaaaa
     1081 atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga cttctttaat
     1141 ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt ccttgtcttg
     1201 attagaggtg tataaagctt tttattagac atttttaaat tccattacaa tttgcttata
     1261 aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg tgattaggag
     1321 tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca ttatattaat
     1381 ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt ttcgtggaga
     1441 gatcccatta cgaaatttag aaactttcca acactcagca attattattt cagtgatttt
     1501 tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga tcagtgacat
     1561 cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg tagtgacttt
     1621 tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg ttctcggtac
     1681 gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg taagtcgaga
     1741 taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg
     1801 ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact attatgcaaa
     1861 catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata tcgaagaaac
     1921 tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt ttttgaatac
     1981 aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag atgaaagtat
     2041 tattgaagca tctggattta gaatccaacc tgaatatgaa ctattcaaac gtttgtttga
     2101 tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat tcacagcaat
     2161 tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga tcacaaaaaa
     2221 tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg aagcaaaatc
     2281 tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta aatttattcg
     2341 agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg atatgtcgat
     2401 cgtaggacca cgtcctgaac gttctttctt cgtcgatcag ttcaatgcag aaaaccctta
     2461 ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag tctatggaaa
     2521 atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca aaaaatattc
     2581 attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg ataaaatgtc
     2641 ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg cttttccgaa
     2701 tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat tcagcataat
     2761 cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc taaaagaatt
     2821 ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc aaacacagga
     2881 aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta cgaccaatca
     2941 agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat atctcttatt
     3001 cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc atttgctaga
     3061 aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa tggacgaggg
     3121 aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa ctcacgaaga
     3181 gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg tatggaacaa
     3241 atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt ataatagttg
     3301 tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga tcatgaaccc
     3361 aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc aataccgacc
     3421 taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa cagacgatat
     3481 taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta ttttttctat
     3541 ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc agatattgag
     3601 tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag caaaaaaaat
     3661 aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa aagttggaag
     3721 cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat attagttaat
     3781 ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt taggtggagc
     3841 agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc aatttgattt
     3901 tttgatttat gaagattatg cagatgttca agactatcat tcagagattg aaagattagg
     3961 aggccgtatt tttgttgtac ctaatcctaa gaaaaatatt tttaaatatt taatagaagt
     4021 gaataagtta ctaaaaaaag aatctttttc tatagttcac aatcaggttt attttggcgg
     4081 gggaattaat ttatggttag ccaaaaagaa tggaatcaga cagagaattg ctcatagcca
     4141 tgcaactgaa gatggaaaat ctcaaaatat tgtaatgaat gtattgcgta aatttttaac
     4201 caaattgttg ttacaaaatg caacagacta ccttgccgtt agtcaagaag ctggtgaaag
     4261 tctgtttcaa aaccatccct ttgagatagt tcataatggt attgatttag agctttatag
     4321 taaaaattca gaagctaagg ttaataagag aaaagaattg gatatctcaa tgagtacttt
     4381 tgttgtggga aacattggac gattagagaa acaaaaaaat caatcttatt tgttagagat
     4441 ttttttagaa atactaaaaa tgaattctga ttcttacttg attttagtag gtgaagggtc
     4501 acttagaaat aaactgaaag ccaaagcagt agaattggat atagagaaaa gagtaaaatt
     4561 tttaggagaa agaaacgata ttcccgagtt attatctgtg atggatgttt ttgtaatgcc
     4621 ttctctttat gaaggtctgc caatggttgg attagaagca caagcagcaa aattaaaatt
     4681 agtcttagca gatactattt ccaaagatac aaagttaaca aataatgtgg attttgtatc
     4741 attagatgaa acaccacaag aatgggcaaa aaaagtaata aaagagccgt atgctaataa
     4801 aataacacaa gaactaaaga gttatgatgt ttcgtacact actgaacaaa tggagagtat
     4861 atatagtaga gatcattaaa aatgttcaag atattttagg agtgattatt tttgaaaata
     4921 tgttatttga tattggcata caatgatatt ggcaacttaa agagattggt caatcgattg
     4981 aacgaaaatg cagtttttat tattcatata gataaaaaaa ctgatataga accatttatt
     5041 gaagcattta aatcatatga taatgttgaa tttttatgta ataaaaaccg tgttaaaatt
     5101 tattggggtg gtttttccat tgtacaagca gaaatcaatt tagtaaagag agcattacaa
     5161 aatgagaaat atttaaaata tgtattatta tctggtgctg attatccaat caaagataat
     5221 gagtatattt ataattattt taaaaagaat agttcagtg
//
LOCUS       DAYYJC010000132         5220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000132 DAYYJC010000000
VERSION     DAYYJC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.136"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACHA9N_002569"
     CDS             complement(<1..1256)
                     /locus_tag="ACHA9N_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9085988.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACHA9N_002570"
     CDS             complement(1270..2487)
                     /locus_tag="ACHA9N_002570"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9085989.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACHA9N_002571"
     CDS             complement(2477..4072)
                     /locus_tag="ACHA9N_002571"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9085990.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACHA9N_002572"
     CDS             4197..4982
                     /locus_tag="ACHA9N_002572"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFM9085991.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACHA9N_002573"
     CDS             4975..5217
                     /locus_tag="ACHA9N_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9085992.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAYYJC010000133         5116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000133 DAYYJC010000000
VERSION     DAYYJC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.137"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1091)
                     /locus_tag="ACHA9N_002574"
     CDS             complement(243..1091)
                     /locus_tag="ACHA9N_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9085993.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1255..2157
                     /gene="gnd"
                     /locus_tag="ACHA9N_002575"
     CDS             1255..2157
                     /gene="gnd"
                     /locus_tag="ACHA9N_002575"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9085994.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="ACHA9N_002576"
     CDS             2159..3160
                     /locus_tag="ACHA9N_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9085995.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="ACHA9N_002577"
     CDS             3157..3441
                     /locus_tag="ACHA9N_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9085996.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="ACHA9N_002578"
     CDS             3474..4271
                     /locus_tag="ACHA9N_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9085997.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..>5116
                     /locus_tag="ACHA9N_002579"
     CDS             4285..>5116
                     /locus_tag="ACHA9N_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9085998.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
BASE COUNT         1642 a          839 c         1100 g         1535 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gatttt
//
LOCUS       DAYYJC010000134         5080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000134 DAYYJC010000000
VERSION     DAYYJC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.138"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..782)
                     /locus_tag="ACHA9N_002580"
     CDS             complement(204..782)
                     /locus_tag="ACHA9N_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9085999.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(802..1470)
                     /locus_tag="ACHA9N_002581"
     CDS             complement(802..1470)
                     /locus_tag="ACHA9N_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9086000.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1485..2555)
                     /locus_tag="ACHA9N_002582"
     CDS             complement(1485..2555)
                     /locus_tag="ACHA9N_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9086001.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2726..4090)
                     /locus_tag="ACHA9N_002583"
     CDS             complement(2726..4090)
                     /locus_tag="ACHA9N_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9086002.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4087..>5080)
                     /locus_tag="ACHA9N_002584"
     CDS             complement(4087..>5080)
                     /locus_tag="ACHA9N_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9086003.1"
                     /translation="EYSDLSGNTFLDLLQKEFSFTITEEGRLKFEIWLSIMKKRMRCT
                     KKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNETLVFYLFMISNFTLD
                     THNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEEISQKIALTVMQNHAH
                     FAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIVDFTEVDEENARFELF
                     ARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLLNTIKYDLVEYEKEKQ
                     YDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECYLEKLNLRMNKT"
BASE COUNT         1490 a         1034 c          808 g         1748 t
ORIGIN      
        1 tctacaaaca attaatttct tcgttattaa attaaaacac ttcctggaac accgacataa
       61 taaataaaaa tataccctga taatcatgag agaataacaa atttgtattt atgaaaaact
      121 acctatataa aaaaaacaag atataacgat cttcgtcata tcttgcttct tcacctaact
      181 atttttctcc ctatataaca gctttacgct tctcctaata caccattcaa atataaatcg
      241 atcaattgcg ccacatttag cggttcttcc acaggtcttt tcgaccgagt gatgtaaggt
      301 gctaacacta agaaaaaatg ccttgctgcg atttcttttg aaatcccatt tcttagcaca
      361 gattggtttg ctgaaaagat ttcttcaaaa ggttcacgat aatttttctt ccatagaaag
      421 aaaagatgat ctcttgtttc ctttgaaaga ttgctttcaa gatcgtgaag catcagaaac
      481 aaatcgacca gatgtgtctc aatcaaaaac tgcgccattt ttgtcaaggt tacttgaaat
      541 cccgtttcgc ttgtttgaac gatttctctc aaagcttttc caacatcttt taagtggttt
      601 tctaaaactg ccgtatatag tttttcttta tcaccgaaat aatgatagag attgggttgg
      661 gtgatcccaa ctttttgtgc gatctctctt gttgaagtgc cgagatatcc cttggacata
      721 aacaagtcag tcgcttcttt caaaattttt tgctgtgtat ctttgttgta tcgatggacc
      781 atttttccac ctgctttttg gttattcttt ttctttcaat acaccatctt ccatcataaa
      841 tattctatcg catttgtcaa tcagccgtgt atcgtgagta accatgatcg ttgctttatt
      901 tttgtctttt gattcttttg ccaagatatc gactacttct cgtgcctttt ctgaatccaa
      961 actagcagtt ggttcatctg ctagaataat ggacggatca ttgtacaatg cacgagcgat
     1021 tgcgaccctt tgccgttctc caccagatag ttcttctgga tacttgttga tcaacttatc
     1081 cacgccaagc tgctcaaata attcttttgc ttctttttga cgatgttcat gtttgatctt
     1141 atctactaat aataactgct tttttactgt cagaaaagga accagattgg aagcttgcaa
     1201 aataaagcca atttctttaa aacgtagttt aacgcgttca ctttcttttt gattactgaa
     1261 ttcttgatca ttgataaata cagagccact gctaggcgtc tgcaatccgc ctgccaaagt
     1321 caaaaaagta cttttacctg aaccagaagg accgatgatt gcaacaaatt cccctttatc
     1381 gatactcatg gttgtttctt tcaatgcttt aactacttgg tcgccatctt ggaaactttt
     1441 ttctacttct ttaaattcta ttgctttcat gctcttcctc cttcttatcc aattgccttc
     1501 aacggatcga tttttacgat tgtccgaaca gaaaatagtg cgcctaacac agctactacc
     1561 agcatcaaca agctaatgac gccaaaaaac agccaattgc tttggaatgg aaccgcatca
     1621 ggtagaacaa gggatgttcc cactgttcct aatagaccaa gaagaatacc aacaaatgat
     1681 aaaatgaatg tttgtgccac tacagaacgc gcaataaatc ctcctgtgat cccttgtgct
     1741 ttcattacac caaagatatt aatcttttgc atcgtcagta cataaatgaa aatcccgatc
     1801 acaatagccg caataacgat caaaaatccg atcataaatc caaaagtcaa tacttgcgca
     1861 ttatatccag gtaattcatt gatgaattta gaaattttga tctgttctaa atcttcaggt
     1921 aaattactga ttttacctct gacaactatt gcgttaatcc gtgcattttc acttgtatct
     1981 tctttttcga agcggatctc ttggtaagca ttgatggttg tatagagaac tggtgagaca
     2041 ttgaatttgg catgatccgt aaagccagtt agtttaaacg tcttgtcact accagataac
     2101 ttaactgtat ctcctacggc tagaccatat tcttctttca aactaatatc gcctactgcc
     2161 tcatcatcgt tatcaaaagc ttttccttca acaagattgg gcatgatgaa ttgattttta
     2221 tcgataccga agaagctaac attgattttt ccttcttctg tactgtcttt actagtcacg
     2281 actccaggtg tctgggcaag atacgccact ttcccacctt cgacttcttc tgctatcttc
     2341 ttagtaatca tggacatccc tagatttgaa tttgcatctt tcgaaagcac aatcgaatct
     2401 gcctcccact tgtcgactgc tgttcgatta tcttgagcta agccataagc taacccagtc
     2461 aaaaaaaata ccaggtaggc gatcaaaaac atcacccctg caactaacgc ataacgtaac
     2521 tttgaatgca taatttcatt taatgctaaa aacatttaac tcctcctcct gaaataatca
     2581 ttttatacaa caattttatc acttgataaa tttttggcga atattatgct caaaaaattc
     2641 ttccgctatt tcattatatg aattttttat tctcgattga ttaatattta ttatatcccc
     2701 acacaataaa agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc
     2761 tcttagctag tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtgttcag
     2821 acagaaatcc aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat
     2881 ccgcaagcag gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta
     2941 tttctaatgt atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcatcgt
     3001 attgtggaaa tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg
     3061 cttcatcttc tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta
     3121 tcttttgtct gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg
     3181 ccatatgtac agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac
     3241 tgactgggac gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat
     3301 tgactatcac agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag
     3361 gacgagtttg ggcagaaaaa atgaccatat ctgcctttat tgcttgagat agaagttgta
     3421 cggatactac tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat
     3481 tgatttgctt agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc
     3541 ttgataaaac attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag
     3601 catctgctaa ttccaatcca atataaccag caccgaagat cactacttct tttgcttctt
     3661 ttaaagcggc aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag
     3721 cttcaatttg tttatacaag gtgacaggag aggaacctgt tgctacgatt aatttatcat
     3781 aggttatggt ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat
     3841 caagttcagt tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca
     3901 tgttatgggc aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat
     3961 ttgaaacata gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg
     4021 gttgaagttt tcttagattt agggcagctg tgatgcctgc atgggaagca ccaatgatca
     4081 cgattttcat gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa
     4141 ttggttcaaa taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc
     4201 ggacttctgt gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc
     4261 tttctcgtat tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc
     4321 tttcacgata tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa
     4381 ttctaactgc ttgatcagcg tccgcaaaat agatgcatag cgagcaaata attcaaagcg
     4441 ggcattttcc tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct
     4501 ttcacataaa accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc
     4561 ttggccaaaa atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac
     4621 tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc
     4681 tatcgattgt ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct
     4741 tatcaaaaac tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa
     4801 aagataaaaa accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg
     4861 ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag
     4921 attgctataa tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag
     4981 ccatatttca aatttcaatc gtccttcttc tgtaatagta aaactaaact ctttttgcag
     5041 cagatctaaa aaagtgtttc ctgataaatc actgtattct
//
LOCUS       DAYYJC010000135         5061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000135 DAYYJC010000000
VERSION     DAYYJC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.139"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACHA9N_002585"
     CDS             complement(205..660)
                     /locus_tag="ACHA9N_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFM9086004.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACHA9N_002586"
     CDS             complement(1063..1236)
                     /locus_tag="ACHA9N_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086005.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACHA9N_002587"
     CDS             complement(1917..2456)
                     /locus_tag="ACHA9N_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9086006.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACHA9N_002588"
     CDS             complement(2472..3395)
                     /locus_tag="ACHA9N_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9086007.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACHA9N_002589"
     CDS             complement(4523..4678)
                     /locus_tag="ACHA9N_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086008.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACHA9N_002590"
     CDS             complement(4697..>5061)
                     /locus_tag="ACHA9N_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFM9086009.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAYYJC010000136         4878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000136 DAYYJC010000000
VERSION     DAYYJC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.140"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACHA9N_002591"
     CDS             complement(<1..258)
                     /locus_tag="ACHA9N_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086010.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACHA9N_002592"
     CDS             complement(274..924)
                     /locus_tag="ACHA9N_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086011.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACHA9N_002593"
     CDS             complement(935..2107)
                     /locus_tag="ACHA9N_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9086012.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACHA9N_002594"
     CDS             complement(2126..2998)
                     /locus_tag="ACHA9N_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9086013.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACHA9N_002595"
     CDS             complement(3020..3940)
                     /locus_tag="ACHA9N_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086014.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACHA9N_002596"
     CDS             complement(3940..4209)
                     /locus_tag="ACHA9N_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086015.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACHA9N_002597"
     CDS             complement(4206..4586)
                     /locus_tag="ACHA9N_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFM9086016.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1291 a         1061 c          799 g         1727 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataag
//
LOCUS       DAYYJC010000137         4867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000137 DAYYJC010000000
VERSION     DAYYJC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.141"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1762)
                     /locus_tag="ACHA9N_002598"
     CDS             complement(<1..1762)
                     /locus_tag="ACHA9N_002598"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9086017.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQS"
     gene            complement(1722..3475)
                     /locus_tag="ACHA9N_002599"
                     /pseudo
     CDS             complement(1722..3475)
                     /locus_tag="ACHA9N_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3585..4154)
                     /locus_tag="ACHA9N_002600"
     CDS             complement(3585..4154)
                     /locus_tag="ACHA9N_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9086018.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4172..>4867)
                     /locus_tag="ACHA9N_002601"
     CDS             complement(4172..>4867)
                     /locus_tag="ACHA9N_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9086019.1"
                     /translation="FNSSIEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGL
                     VGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELAL
                     GNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTS
                     GLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVED
                     STLIEQIFNDITK"
BASE COUNT         1617 a          925 c          662 g         1663 t
ORIGIN      
        1 gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc tttatgagat
       61 ttttgtatat ttctgacctt cgatacaact cattatgagt accttgatca actagttttc
      121 cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga tgcgatataa
      181 ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt ttttcatttt
      241 ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt ttcaaaaaag
      301 cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg ttttctccga
      361 ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta gctaaacgag
      421 ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata ttttccaaaa
      481 tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta aacaaagaag
      541 ctggagatac acgtttaata tcatacccat caattaaaat acagccttca tcataatcat
      601 acagtctaga cactaatttc aagatactag ttttcccgga accactagca cctactaacg
      661 cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg gtattgttat
      721 catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga ctatctgatc
      781 cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga gctaaataaa
      841 atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt attgcggcta
      901 gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag tgaacaccta
      961 ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc atgactgaaa
     1021 aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt ttgaaaagtc
     1081 tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat tctatagttt
     1141 cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg gctttcgttt
     1201 gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa ataacagcta
     1261 atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa aacagagcca
     1321 taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca acgtcagaca
     1381 tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat ggtaaatttg
     1441 acaaatcgtc tgcaatttgc actcttaaat gcgcactttc tttataagta ctattatata
     1501 agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaaca aacgatacgg
     1561 caaaataaag ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc agcatcatta
     1621 taatgaatgc tggtaccata ttaatgcagt atacaaaaaa actagaaata gaagctttag
     1681 ttaaagcttc tgcgccctga tcacttaaag caaatttgtt tttataaaaa cttttcattg
     1741 tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata cacttgatgt
     1801 ttagccatta attcattatg atttccacgt tctactattt tcccttctga caagacaaga
     1861 atttcatcta cagatttaat gctggataat cgatgagcta tcataataac tgttttttct
     1921 ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc aatagatgca
     1981 ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc aatagctata
     2041 cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt atgctctctt
     2101 tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc aaatacttta
     2161 cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg attgaatagt
     2221 ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga atagtgattt
     2281 aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc tgaaattagt
     2341 ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat tcttttttgt
     2401 tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa gctgacattt
     2461 tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag ttggtcttgc
     2521 ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc aaacaagtgc
     2581 atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa aaaaaacaac
     2641 atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa aaaggttata
     2701 ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac aaaaggtgtt
     2761 ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc tttattcaag
     2821 gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc gcggatatat
     2881 tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat aaaatttgta
     2941 ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat tagaaccaat
     3001 ccaattttaa agttaatgaa aaagcctaag gcaactgata aaacaggtgt tactatggct
     3061 tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc gttgtcatca
     3121 attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact tgcttctgtt
     3181 aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag gatatgagaa
     3241 aataagccag aaaaaatata aaataaagct ctaaagttaa ccaagcaaca gtttgaaccg
     3301 cataactctt agctaaatat tcatctccta caataattag agactgaaga aatttgtaaa
     3361 tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa gtggctaacc
     3421 atccgtagta tttcatttta gggacataac taaataattt tttgtatact ttcatttttt
     3481 cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct ttccgtaata
     3541 gcgtaaatta gagatgtaca aacagataaa aggtaaacta cgttctattt tttgtatttt
     3601 tttaagactt ttcttccaat caatacacca atcatagata aacttgctac aactaacact
     3661 gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt tgcagcagat
     3721 tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat aggaatccac
     3781 tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg aattgctcga
     3841 tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat cgaactgata
     3901 aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac aattgtgtaa
     3961 atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat gattccagat
     4021 aataatccaa aaatcccggg ggatatgctg tgaccaaacg ccccaccaat actactaacc
     4081 attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat aaaaatataa
     4141 tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta aaaatctgtt
     4201 ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca ccatttttta
     4261 aataaataat tctattgcat gttttataaa gaaattccgg atcatgagta gctataacta
     4321 ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata ttaatataat
     4381 ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat gaagctatag
     4441 aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga tctttcattt
     4501 tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact atttcattcc
     4561 ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt tcttgcatta
     4621 ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg gtagggcgaa
     4681 taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc cctaccaaac
     4741 caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt tcataagtaa
     4801 tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact tcaatcgaac
     4861 tattaaa
//
LOCUS       DAYYJC010000138         4788 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000138 DAYYJC010000000
VERSION     DAYYJC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.142"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACHA9N_002602"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACHA9N_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM9086020.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACHA9N_002603"
     CDS             1019..1183
                     /locus_tag="ACHA9N_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086021.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACHA9N_002604"
     CDS             2141..3118
                     /locus_tag="ACHA9N_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9086022.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACHA9N_002605"
     CDS             3128..3625
                     /locus_tag="ACHA9N_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9086023.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACHA9N_002606"
     CDS             4001..4282
                     /locus_tag="ACHA9N_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086024.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACHA9N_002607"
     CDS             4284..4700
                     /locus_tag="ACHA9N_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086025.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAYYJC010000139         4740 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000139 DAYYJC010000000
VERSION     DAYYJC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.143"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACHA9N_002608"
     CDS             <1..712
                     /locus_tag="ACHA9N_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM9086026.1"
                     /translation="TPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLL
                     STGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSD
                     HSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLG
                     DGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGK
                     KVKRPISADSVLLEEDVE"
     gene            709..1857
                     /gene="neuC"
                     /locus_tag="ACHA9N_002609"
     CDS             709..1857
                     /gene="neuC"
                     /locus_tag="ACHA9N_002609"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9086027.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1830..2507
                     /locus_tag="ACHA9N_002610"
     CDS             1830..2507
                     /locus_tag="ACHA9N_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9086028.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2458..3639
                     /locus_tag="ACHA9N_002611"
     CDS             2458..3639
                     /locus_tag="ACHA9N_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9086029.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            3652..4629
                     /locus_tag="ACHA9N_002612"
     CDS             3652..4629
                     /locus_tag="ACHA9N_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9086030.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            4629..>4740
                     /locus_tag="ACHA9N_002613"
     CDS             4629..>4740
                     /locus_tag="ACHA9N_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9086031.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYS"
BASE COUNT         1734 a          601 c          925 g         1480 t
ORIGIN      
        1 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
       61 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
      121 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
      181 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
      241 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
      301 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
      361 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
      421 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
      481 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
      541 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
      601 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
      661 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
      721 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
      781 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
      841 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
      901 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
      961 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     1021 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     1081 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     1141 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     1201 tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta
     1261 cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt
     1321 ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct
     1381 ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat
     1441 gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat
     1501 attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc
     1561 ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt aataaattta
     1621 ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa
     1681 aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa
     1741 aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat
     1801 caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat
     1861 atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa
     1921 ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc
     1981 aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa
     2041 aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa
     2101 aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa
     2161 aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg
     2221 ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa
     2281 ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg
     2341 taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt
     2401 gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg
     2461 gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca
     2521 agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg
     2581 atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt
     2641 tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga
     2701 ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca
     2761 gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca
     2821 tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa
     2881 ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt
     2941 ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt
     3001 tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac
     3061 ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat
     3121 gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat
     3181 agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa
     3241 atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa
     3301 gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat
     3361 cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta
     3421 ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg
     3481 caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa
     3541 aagttattta atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa
     3601 gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata
     3661 ggaattattg gtttaggttc catggggaaa agaagactta gactcattca agaacacttt
     3721 aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag
     3781 gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat
     3841 tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat
     3901 ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc
     3961 gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa
     4021 gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat
     4081 gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca
     4141 aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat
     4201 acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt
     4261 gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta
     4321 agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt
     4381 ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt
     4441 atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt
     4501 attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg
     4561 gaaccccaat atacttttga aaaagataag tatattatta acttaattaa cgacattgag
     4621 gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag agacatctaa
     4681 aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca gcttatagta
//
LOCUS       DAYYJC010000140         4712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000140 DAYYJC010000000
VERSION     DAYYJC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.144"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACHA9N_002614"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACHA9N_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACHA9N_002615"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACHA9N_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9086032.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACHA9N_002616"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACHA9N_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACHA9N_002617"
     CDS             1786..1869
                     /locus_tag="ACHA9N_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9086033.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA9N_002618"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACHA9N_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9086034.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACHA9N_002619"
     CDS             complement(2994..3857)
                     /locus_tag="ACHA9N_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9086035.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACHA9N_002620"
     CDS             complement(3859..4131)
                     /locus_tag="ACHA9N_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9086036.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACHA9N_002621"
     CDS             complement(4148..4363)
                     /locus_tag="ACHA9N_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9086037.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="ACHA9N_002622"
     CDS             complement(4455..>4712)
                     /locus_tag="ACHA9N_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFM9086038.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DAYYJC010000141         4680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000141 DAYYJC010000000
VERSION     DAYYJC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.145"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..541
                     /locus_tag="ACHA9N_002623"
     CDS             <1..541
                     /locus_tag="ACHA9N_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9086039.1"
                     /translation="PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLE
                     RQIMLECCAKMNNQKKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEV
                     WQWLEDNNTHLERFRWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHL
                     MLDEQNVVLTTHPKYFKNI"
     gene            complement(1377..2831)
                     /locus_tag="ACHA9N_002624"
     CDS             complement(1377..2831)
                     /locus_tag="ACHA9N_002624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086040.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2832..3131)
                     /locus_tag="ACHA9N_002625"
     CDS             complement(2832..3131)
                     /locus_tag="ACHA9N_002625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086041.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(3434..3805)
                     /locus_tag="ACHA9N_002626"
     CDS             complement(3434..3805)
                     /locus_tag="ACHA9N_002626"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9086042.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(4139..4402)
                     /locus_tag="ACHA9N_002627"
     CDS             complement(4139..4402)
                     /locus_tag="ACHA9N_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086043.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1478 a          930 c          726 g         1546 t
ORIGIN      
        1 tccaatacgt gaagcattga ttcaactccg tcatcaagga cttgttgata caattcctca
       61 aagtggaaat tatatctcat taatcgactt agatttagca aaaaatgctc gttttgtcag
      121 agaacattta gaacgacaaa ttatgctaga gtgttgtgcc aagatgaata atcaaaagaa
      181 acaagtttta gaaactataa ttgcagaaca agaaaaagct gttagtcaaa agaatgctcg
      241 tagttttttt cataatgata atttatttca taaagcttgt tttgaaattg cagaacggga
      301 agaagtatgg caatggttgg aagataataa tacgcacttg gaacgatttc gttggttacg
      361 agtaaaaacg gaaggattaa aatgggaaaa aattatggaa caacatcatc aactatttaa
      421 agccttgacc atgcaagacc ctgaagaagc taacttctta acatccttgc atcttcattt
      481 aatgcttgat gaacaaaatg ttgtattgac tactcaccca aaatatttta aaaatatata
      541 actgctaaat ttcttttttt taatttattt acatttctga ttactacaga aaactaacta
      601 ctttttttat gtaaagttct gtcaaatata gtcactttat attactttct cttgagtaaa
      661 aaaacatgtg aggtcgtaaa caaagattga actatctaca ttcatactct ataatctgtt
      721 tatttgcaga ctgataatag caaaagaaaa cttataatca aataatatca acatttgatc
      781 acataatttg agattgaatg ctgatttacc tatgttttac gtaatggtct ttagttattt
      841 ttgctcataa aatgcataaa acacgcgttt ttatgtgtgc ttgaaatttc ttggctctat
      901 aatttctaag actgataaaa atattctatc ggttcttttt gcaaataaaa aaacatatag
      961 aagctccatt agattgaatc accacaacac aatcaaagga gcattccgta tgcttgattt
     1021 tcattctata aaactacttt taggaatcaa agaaaatcac aacggttcaa actaaaagca
     1081 agaatatccg aagtaccatt gtttcatcga tcatgagaaa agcattcacc tctgtctgca
     1141 gatgttctta tatcgctctg tcagtcctat taataaagta ctgttttgtt ttcaaaaaaa
     1201 ctattacgag tactttttaa gagaatgcct tgcaaatatt catcacacac cgttatataa
     1261 gtcaaatagt ttctttcagt cgtcaagaaa agattctcct gtccttggca ttatcagtta
     1321 agtagcaact taattctcgg acttgttcta ctctagcaaa atttattttc atcaacttac
     1381 gtccaaatgt ttttttgaat aattacttca tcgcgggtct ggaagacttg cctcattcga
     1441 taagtcacta gcttttggcg tccttcagga tttctagtat acaccaactg acatttgggc
     1501 agcgacgatt gatttaattc gtttagaaaa aggttagcga tttgcttatt tcggccaaag
     1561 cgttctggta ccgtcagaac tatctttttc ccttcgatta aataccgcgg attctccagc
     1621 ggtgctaaaa gctccctcat agcttcagta aaaagctgtc cttctcgata gccagcattc
     1681 tcaagatagc atctaaattc ctgctgatca gccgctacga ttacttttct atcactaact
     1741 tcaccaaggc gctgcaacgt cgtttgaacg catatggcca agcttttttt gcgggcacat
     1801 tccttttccc atagcttttg tcgcctacga atttggaaat attccagcaa gttaccttta
     1861 aatatagctg accagaagct tagaggctct atatttttta ctactatcgg caatgaagtt
     1921 tcttgttttt gctttacctg atcgactttc ctaacgacga ttggcataga taggtgccag
     1981 ccatcagcta aagcagtact ccattgttct ttcaaccgac ttcgttcaca agaaaaatag
     2041 aacatcgact gagtataagt ttccatcgtg gtggtcgtat attccagtgg taaagcaaag
     2101 cgatcgccac cactctcaat ttttggtgaa gattcaaagg tcagacctga aatattgccc
     2161 aaacgccgta gcattctttt caaatcaggc tgttcagaca gaggaactcc cggtaatcca
     2221 atcgctaaat gccaaatgtt cgttacttta ttttgtgcac ctaggcgtaa gccccgccct
     2281 cgtagttgtt gtgtttgcac aaaagtggaa gcttgattag ccaagatcaa cagattaatt
     2341 gcaggacaat cccagccttc tcctagcaaa ctaacggttc ctaccaaaat attcaacttc
     2401 ttttcggtta ataggcgagt aactgcttgc atcaatgctt ggcggttttt gtcatccacc
     2461 gtcaaataaa aataacccga catataagga tgttcattca tacccgtcgc tagttctgcc
     2521 agctcaacag ttactagcat tttcggtaac agtaagaact ctccacagat ggcaccgatt
     2581 tgctgagtgt tacctaatag acgaacaact tcatcaaaca aaggaaccac gccataagct
     2641 aaggtaccat cactgcctgc taatacctcc ttgcaaatac gatccatcaa aatgaccgct
     2701 gtcagttctt gcttcagctg aacttcttct tttaacaaaa tttgacaaac tgcttgcttt
     2761 ttctcaggaa tcgcctgact accaacagtg tctggatact ctggaaacaa atgcagcgac
     2821 tcatcaaaca atcatccagc ttttttcaat tgataaaaaa taggcaattc cggttgatct
     2881 tcaaacatct gctgataaat agtgatcagc tcttttttac ctaaagatgg aactgaagct
     2941 tttttacctt gctgcttaga actgcctaat agctgccaat gttcgggtat caattcatag
     3001 ttatgatgat tcaagtaaat taatgcacta gtgaacagct caaaattttc ataaatttcc
     3061 ttcgaatgct caagcggatg ctgaacaaat tctaaagaaa gcaaataatc caccgtctct
     3121 tggctttcca ttaacgcttc caccagctca ttttgtttct ctaaaaaggt aaaataatgc
     3181 tgagtagttt cctgattagc cggaatcaaa tatagaaaat cctgatgagg acatagaacc
     3241 tcctccttta ccaactcggg aatgctaatt tcttcgtcaa tcaggccatt ttaactcaat
     3301 atattgcttc cactccgtct gagagctatc aatcggaggc gtggctgtca aggaaatcgt
     3361 caacagattc ggttgttttt tcttcaagtc aatcaacaat tcaccccagc ttttttttaa
     3421 atgatgagct tcatcaaaaa actaacagct ccagctgatc accataagtt ggctcctcct
     3481 tcagtcggtt gtaaatggtc tgatacgttt ccactgtaat atgactagga tgttcaatcg
     3541 ccccagaaag atagcaacta tctgctggat ttactaagaa atattcctcg attgttgtta
     3601 accactgttt cttcaatatt agggaaggaa ccaaaattaa ggttttacaa tccaaacgtc
     3661 ggatcatctc aattcctaaa aaggtcttgc cagcccctgg tggagccact agatggactg
     3721 aattgttatt taaataagta gcaaagttag tcaatatacg cttctgatag ctacgccact
     3781 gaattttttc atcagatagc cacatcccag gtccccctct cacgtatttt ctctatcata
     3841 atctaaatga atacagcaaa aaaaagattt cttcaagaat atgtgttttt atagtaatat
     3901 tttacttact ctaaactcaa atgaaaaggt aaaaaaactg gtagtacaat cttttaaatt
     3961 ctcattgttt tgtacgttct ttactatcaa aaaaacaaaa aaaacgcgtt tctacgcgtg
     4021 accaaaattt ttagctctat aatttctaag actgatagaa aatcattctg tttttgaagt
     4081 cttttttcat aaatactact tgatttattg tgtatattgt ataaaagatt gttcataatt
     4141 attttttata attaaaattt ggtattgatg accagcgatt ccgtatataa tgcgcaatca
     4201 aattaagctt tctgtctctg gtaaatattc ctgttctgtt ttcttgtact cgtaagattt
     4261 cttcacttat cgcaaaattc ataaaattcc acactttttc ataaacaaaa tgtacacatt
     4321 tatctattac ttcataatag tctacttgat attcttctgt aaacatggtt gctactgtat
     4381 ctgcaccata tttgataaac atttgggcta ctcccttaaa ttgagaaaag cccattagtt
     4441 tatctactaa tacttctaaa atttcttgct ttcttcttta acatcgtccc gtaaggaatc
     4501 atgtagtatg ctggaggaag ttcattttct aaataattgt atcgcttcaa ttgctcaaag
     4561 gctattctct acaattaatt acaccaatta ctttatcttc acttatataa gatttctaaa
     4621 catttttcgt caaatgaaga gaaaatattt ttttaaatga tcttgctcaa gatttgtcaa
//
LOCUS       DAYYJC010000142         4646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000142 DAYYJC010000000
VERSION     DAYYJC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.146"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..717)
                     /locus_tag="ACHA9N_002628"
     CDS             complement(133..717)
                     /locus_tag="ACHA9N_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9086044.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="ACHA9N_002629"
     CDS             complement(928..1131)
                     /locus_tag="ACHA9N_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086045.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="ACHA9N_002630"
     CDS             complement(1141..1563)
                     /locus_tag="ACHA9N_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086046.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="ACHA9N_002631"
     CDS             1801..2415
                     /locus_tag="ACHA9N_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9086047.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="ACHA9N_002632"
     CDS             complement(2580..3404)
                     /locus_tag="ACHA9N_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086048.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="ACHA9N_002633"
     CDS             complement(3428..3583)
                     /locus_tag="ACHA9N_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086049.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="ACHA9N_002634"
     CDS             complement(4042..4299)
                     /locus_tag="ACHA9N_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9086050.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="ACHA9N_002635"
     CDS             complement(4292..4561)
                     /locus_tag="ACHA9N_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9086051.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DAYYJC010000143         4634 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000143 DAYYJC010000000
VERSION     DAYYJC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.147"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACHA9N_002636"
     CDS             174..518
                     /locus_tag="ACHA9N_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9086052.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACHA9N_002637"
     CDS             519..938
                     /locus_tag="ACHA9N_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9086053.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACHA9N_002638"
     CDS             complement(1030..1380)
                     /locus_tag="ACHA9N_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086054.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACHA9N_002639"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACHA9N_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            2932..3498
                     /locus_tag="ACHA9N_002640"
     CDS             2932..3498
                     /locus_tag="ACHA9N_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9086055.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ACHA9N_002641"
     tRNA            3643..3730
                     /locus_tag="ACHA9N_002641"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="ACHA9N_002642"
     CDS             4064..>4634
                     /locus_tag="ACHA9N_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFM9086056.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DAYYJC010000144         4626 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000144 DAYYJC010000000
VERSION     DAYYJC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.148"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(177..788)
                     /locus_tag="ACHA9N_002643"
     CDS             complement(177..788)
                     /locus_tag="ACHA9N_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9086057.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(911..1036)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1118..1834)
                     /locus_tag="ACHA9N_002644"
     CDS             complement(1118..1834)
                     /locus_tag="ACHA9N_002644"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9086058.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1840..2409)
                     /gene="scpB"
                     /locus_tag="ACHA9N_002645"
     CDS             complement(1840..2409)
                     /gene="scpB"
                     /locus_tag="ACHA9N_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9086059.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2393..3163)
                     /locus_tag="ACHA9N_002646"
     CDS             complement(2393..3163)
                     /locus_tag="ACHA9N_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9086060.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3160..3531)
                     /locus_tag="ACHA9N_002647"
     CDS             complement(3160..3531)
                     /locus_tag="ACHA9N_002647"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9086061.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3550..4437)
                     /gene="xerD"
                     /locus_tag="ACHA9N_002648"
     CDS             complement(3550..4437)
                     /gene="xerD"
                     /locus_tag="ACHA9N_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9086062.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1275 a          998 c          800 g         1553 t
ORIGIN      
        1 acaaacattt tcgtctttta aaaggagaag aatttcacat ttgcaaagaa ttgattgatt
       61 tttatgcata aatcaaaaaa atactcaaaa caaacaaata aaacgtttac attattgcat
      121 gtcttgtatt tttttccaaa aaaaaagacc aagaaatttc ttgatctttt tgatatttat
      181 ttgactgctg tatgacgttg ttcgaactga tgttgttttc gacttaacca tggaagaagt
      241 tgcgcatgca gcactaaaaa gacagcactc acgatgaatc ctttgatcaa gttgaaagga
      301 acgatcccaa tcagtacata gtttgctaaa gacatgccca gcatctgtgt tgcgttcagc
      361 cctaaaaaca ttaagtaaag aggcgtaata acgaaataat ttgcgatact cataaaaata
      421 gttagagcga tggttcctgt aatgacacct aaaaatttgt ttcttccttt atgctgtttc
      481 tttacactaa agaaataata aatcgggaat gtaaagatcg ttgttgataa gaaactagca
      541 gtgtctccta ccaaattatc cggagaaaaa cctgtagtca gcagatgtaa tactgaacgc
      601 aagaacgctg ttgcaactcc ggccgctgga ccgaataaga acatgctgat caataccgag
      661 atatcactaa aatcgatctt caaaaaacta aaagccggca tgatcggaaa agcaaaatac
      721 tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa ccattttctg tacacgggtg
      781 ttcttcattt gaaatcctcc tgttaggact cctcttcagc gaactttttg acgtttctct
      841 ccaaatgaaa aaactctatt ttccagggaa aatagagtta gagttatttg atcaagcgtc
      901 gtcaaataag ctctatcttc tttatccaga ctatactgtc ggtatcggaa tttcaccgat
      961 tcagctattt ttttgcaaaa taggtcgtgg actataacca ccggtcggga atttcaccct
     1021 gcccctgaag acgaacctta tataattttt gtttacatat tcattataca tatctattca
     1081 aaaaacgcaa catctaacaa ttataaaaag taagtgttca ttttgagtgg ttcagcaaac
     1141 tgctgacttc ttttttgttc agttcacggt aatccccagg acgtaggcct tgtaaagtca
     1201 gatctccgta tttttcacgt tttagttttt gcacaggaag accaactgct tgaagcatat
     1261 ttttcacttg atgattacgt ccttcgtgga tcgtcaattc cacgatactg tggtttgtct
     1321 taggatccac tgataaaatt tggaatttag ctggagatgt ttttcgtcct tcgatcttta
     1381 ttccttttgt caaaggtgca agcatcgatt ttgtagcgat cccttttacc tttgcaacgt
     1441 aaactttatc cacttcatgt tttggatgtg tcaggcgctg agcaaaatct ccatcatttg
     1501 tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc tacaggatag atacgttctt
     1561 ttaccatcgg caaaagatcc gtaacaactt ttcttccttt gtcatctgca acagcagaaa
     1621 taactccttt aggtttgtac aataaatagt aaccgtattc ttcttgataa attgggactt
     1681 catccacttc cacagtgtca tgtttcccaa cttttatccc taattccctt acaacttgtc
     1741 cgtttacttt tacacgtcct gcaaggatga attcttctgc ttttcgacga gaagtgatgc
     1801 cagcgtgggc aattactttc tgcaatctct ccattacaat tattctcctt tattcattcc
     1861 atcaaagaat agatctggtg acacgtcttc ttcaagtgaa atttccattt cttgtgtatc
     1921 aggcagttct tctaatgatt tcaaaccaaa ataatctaag aagtagccag ttgttccata
     1981 taatatcggc cgacctggtc cttctactct acctttttct tcaatcagtt tatgggcagc
     2041 tagacgctgt accgctcctg aaacctgaac tcctcgaatc tgctcgattt cagctcttgt
     2101 gattggctgt ttataagcga tgattgctaa ggtttcgacg gcggcttgag ataatgtgtt
     2161 tgatgtccct tgcgcatatc gcttaagtaa gggagccagc tcttttttag tcgttaatac
     2221 ataatggctt tccgtttcaa aaatatgcaa agctgagtgc gagttttgag tgtaagattc
     2281 ttttaattct ttcactaaac ttgtgacttc tgcttcgctg agatttaaca gtccgcttaa
     2341 ttcttcatta ctgatccctt cctcgccaac aataaaaaga agggcttcaa gttcacttaa
     2401 tttcgtcatg ttcggtatct cctactgctg gataaagcat aattgattca tagttttctt
     2461 tttgttgcgc aatgatctgc ccttttttca tcaattctag caaagccata aaagtagtga
     2521 caacttcact tttagaaggt gactcaaaaa aagaatcgaa agtacagcca tgcgcatttc
     2581 tttttatacg ctcttcgata aataccattc gttcttcgat cgttgtgtca tcgggcgtga
     2641 tcgttttttc cactttttga gaaaatttct tacgttctaa gattttctgc atggcacgga
     2701 tcaaatcgat ttttttgact tgtcctcgct tcaacggctt ttcagcatcc gctaacccat
     2761 cgatgttcat aggttctttt gtgtaataaa gacttcgttc ctctgctttt tctgtcagtc
     2821 tttcagctgc atatttatat ttacggtatt ctaaaagctg agtgaccaat tcttctcgag
     2881 gatcttcttc ccataattct tcatcgtctg tgccgaattc ttggacagga agcaatgtct
     2941 tgcttttgat tgccatcaat gtagcagcca tcactaagta ttctcctgca acagctagct
     3001 cgagtgtttt cattgtatgg atataattca tatattgctc cgtgatcgta gcgatcggta
     3061 tatcatatat atcgatttcc atttgctgaa tcagatgaag cagcagatcg agaggaccct
     3121 cgaatacatc taatttaata ttgatctccg tcaactcatt cactctttct tctttttctt
     3181 tgctagccat tttccaatgt aaggctctgt gatttcagtt gcacggatgg agacatccgg
     3241 gtacttttcc tgtaattcat ctaacatttg aaagcccata ttctcaccgc gataagacgg
     3301 attcaaagaa atgtcatgga tgatcagctg atcgttcgtt gcccagtaaa taccaataat
     3361 cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa agttcataat cgtcttcaga
     3421 ttcgtacgta tcaatctcct tcaacaaaga agttttacct gtcagtttct tatggaaact
     3481 caatagtccc atagcaattt tacggttttc tttccggtaa tgtgttaaca tcttctcacc
     3541 ttcctatgac taagcccttg gaaaatgctg cttataaaca tccgtcattc ttttcttcgt
     3601 gatatgtgta taaatctgag tcgtagagat atccgcgtga cctaacaatt cctgtactgt
     3661 ccgcaaatca gctccatttt ctagcagatg tgtggcaaag ctatgccgca gcgtatgtgg
     3721 tgttacttct ttgtaaatcc cagcctctcg tactaactgc ttcagatttt tccagattcc
     3781 ttgccgagaa agacctttcc catgattatt gacaaataca tgcatttcag atgcgtcctt
     3841 tacaagtaat ggacgtgctt cgtctaagta gcgttccagc cattggatcg cataatcacc
     3901 taaaggaatg atccgctctt tatctccttt tccaactgtc tgtaccagtc caagagaaag
     3961 atgaagatct cccaatttca atccaatcaa ttcactgacg cgcatcccag tagcatacat
     4021 gacttccaag attgcacgat ctcttatccc taacgttttt gtcgtatcag gtgcttcaat
     4081 cagcttttct acttctgtca gagtcaatgt acttggtaat ttctgtgctt tcttcggcgt
     4141 atcgatatgt tgcatcggat catgatcagt gatacgctct tgccgtaaaa actgatggaa
     4201 tcttctgaga cttgagatca tccgagtaac agttgctgtc gctttttttt cttcatgcaa
     4261 cttctccaga aaacggatga ctaaaaagcg atcgacatcc tgccatgaag caattttctg
     4321 ctcctgtaga aaatgcaagt actgttccaa atcacgttca tagctttgtc gtgtattggc
     4381 ggacaatccc cgttcaatat tcaagtagtg gagataatcg ttgatttgct ctttcataat
     4441 aatccccctc atgcctctct atcatatcag ataccgcttc attctgatag tctttttcca
     4501 taatctgagc aaagaaaaag ccgtctagac actcagacgg actggtttct tttttcggct
     4561 ctataattta tgggactgat gaatcagaat tgattcttca gccccatttt cattttaggt
     4621 attaaa
//
LOCUS       DAYYJC010000145         4473 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000145 DAYYJC010000000
VERSION     DAYYJC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.149"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..1052
                     /locus_tag="ACHA9N_002649"
     CDS             324..1052
                     /locus_tag="ACHA9N_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9086063.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="ACHA9N_002650"
     CDS             1197..3224
                     /locus_tag="ACHA9N_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9086064.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="ACHA9N_002651"
     CDS             3332..4429
                     /locus_tag="ACHA9N_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9086065.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DAYYJC010000146         4425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000146 DAYYJC010000000
VERSION     DAYYJC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.150"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACHA9N_002652"
     CDS             690..1658
                     /locus_tag="ACHA9N_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9086066.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACHA9N_002653"
     CDS             1669..2484
                     /locus_tag="ACHA9N_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFM9086067.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACHA9N_002654"
     CDS             2552..3262
                     /locus_tag="ACHA9N_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9086068.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACHA9N_002655"
     CDS             3298..>4425
                     /locus_tag="ACHA9N_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9086069.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAYYJC010000147         4369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000147 DAYYJC010000000
VERSION     DAYYJC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.151"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1755
                     /locus_tag="ACHA9N_002656"
     CDS             <1..1755
                     /locus_tag="ACHA9N_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086070.1"
                     /translation="NLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSV
                     NFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAV
                     VFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVD
                     IYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSAL
                     IACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFK
                     TDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVS
                     TWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLII
                     GATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKE
                     RPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVV
                     NDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKL
                     KDSRYVTKKEINKKNENK"
     gene            1742..3376
                     /locus_tag="ACHA9N_002657"
     CDS             1742..3376
                     /locus_tag="ACHA9N_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9086071.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3551..>4369
                     /gene="msrA"
                     /locus_tag="ACHA9N_002658"
     CDS             3551..>4369
                     /gene="msrA"
                     /locus_tag="ACHA9N_002658"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9086072.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRK"
BASE COUNT         1535 a          654 c          747 g         1433 t
ORIGIN      
        1 aacttattaa aaaaagacaa ttctagtata ctgaaaatag aattaaaaaa ggagaacaac
       61 aaatctatga aaataagtaa aaaagcattt cttttagtag ttcttatctt acttagtact
      121 ttatattcag taaattttat gagaaatgct caagaaattt atacaacagg tgatttatct
      181 tttcatcttt cacgaatcaa agggttatct tctatttttg aagggccaat caactataca
      241 acatttaaca attatggaga tggactgaac tatttttatc cttttcttac aataattcct
      301 gcagtcgttt tttatggaat ttcaaataac ttaatattaa gttatgttct atatatttgg
      361 ttattaaata tttgtacgat tctgattagt ttttattatg gagaaaagtt ttttaaaaag
      421 atagatgctg cttttttatt tagttgttta tatacttttg caacttatcg aacagttgat
      481 atctattatc gatccgcaat tgcagaagca atcgctctta cgattatccc agctgtttta
      541 tattatgctt atggattgat attcaataga gaaaaaaaag tcatcgcttt atctgtcagt
      601 ataagtctac ttgtgtatac tcatgtctta actacactat tgtgtagtgc attgattgct
      661 tgtatgatta tagttcgttt gtttttaagc aagcaaataa aaaacgatat aaaatttgtg
      721 gtcgtgaatt tagtaaaggc tgtttttttg acttgtgcat taactagcta tttttggtat
      781 cctatgttgc agcagatgaa ttatcaagaa atacacagtc ccttcaaaac agatttgcag
      841 ctagaagcac tttctatttc agatagtatc attcaagcgt taaataacga tattacaaca
      901 tacactatgg ggttattagg attggttgcg ttgatttttc ctttattcat actgaaagaa
      961 atggagaaaa aagaaaaaat catctatgtt ggcgtagttt ctacatgggt atgcgtaact
     1021 aatttatttc catggttttt gtttcaaaaa acaccgataa gcttactcca gtttccttgg
     1081 cgtatcttag gtatccaaat ctttttttct tcaatgatca taacgcttgt ttttatgaaa
     1141 aagactatga ataaaaaata ttcatggtta atcattggcg ctactgtggt gtttctactc
     1201 gtcacgtcta tagcagcaaa agaaaattac gcaaaagtta tccaatcaaa acacggtcat
     1261 attgttatca atgaagaaac actcccacgt tatacaacat cagatatggg aggattatac
     1321 gattacgccc caacagaagc gataaaagaa aggcctcact tagaaaaaca tgaagtaaaa
     1381 gtaggagaat cgtgggaaaa agggaagtat aaagctgcag ataataacat tacttacaca
     1441 gtggcaagca acaaaaagca aaaagtgaca gtaccagtat atgcttattt aggtacacag
     1501 gtaaaagtaa acggtaaagt cgtaaatgac tcttataaaa aacatggctt agtgaatgtg
     1561 gatgtaaatg ctggagaaaa taagatagaa attacaacaa aatatactcc aactgctctc
     1621 atggctttat ggatcagtat tctgacttat tgtttagtgg tgtattcaag ttttaaacga
     1681 tttgcaccta ataagttgaa agattctcga tatgtgacta aaaaggagat aaacaagaag
     1741 aatgaaaata aataaaaaag tagtaatatt gagtggatta attatagtta gtagtatata
     1801 ttctttcatc tttttcaaaa atacacagag tatttatacc actggagatc tttcttttca
     1861 cttatctcga atcaaggggt tatcgactat ttttaccagt ccgattaatt atgagacatt
     1921 caattatact ggatatggtg taaattattt ttatcccttt ctgacttttt ttccagctgt
     1981 aatgttatat tggatgacaa aaaatttgat tgtaagctat attatttacg tttggttatt
     2041 gaacttgtgt acaacactcg tagcttatca ttatggcgag cggtttttaa aacaaaaaaa
     2101 agcggcattt ttatttagtt gtctctatat tttctctgct tatcggacag tagacatcta
     2161 ctatcgttcc gcaattgccg aagctatcgc aataacactt gttatccctg tattatttta
     2221 tgcttatcaa atcatctctg gaaaagaaga aaagtatccg tctgtaaagc tagcattgag
     2281 tatgagtttg ctagtgtaca gccatgtact atcaacatta atgagtacag cattaattat
     2341 catttttatt tttattcgtt tggtttctaa aggtttcaag aatgcagatt ttattgctat
     2401 atttaaaaaa ttatttagtg cggcaggaat gacattggtt ctgacgagtg ctttttggta
     2461 tccgatgttt gaacaaatgc tctaccaaaa aatcaacaaa cctagcgtga caaatttata
     2521 tgctcatgca tctaatgtgt tcgatagtct gactgaagct atgaacaacg acttaactac
     2581 ttactcaatg ggactagttg gtctgttatc tttatgtatt cccctgattt tatttaaaaa
     2641 attaacaaac atagaaaaaa agatttatta tggtacgtgt ttgacttggc tagctacaac
     2701 tagtttggtg ccatggtatc ttctgcaaaa tacgccagcc aaattgttac aatttccttg
     2761 gcggatctta agcttgcaaa taatattctc ttctctgata ttaacgatga tatttttcaa
     2821 aaatagaaga tacaataaga caagagaact attttattta tttatgactg ttttgttatt
     2881 aatcacttta actgtttcta gtaaaaaaaa tttcaatcaa aaaataattt ctcagccggg
     2941 acatattgtt gtaaacaaag aaacagtaga ggcgtatacc actaaaggaa tggattattt
     3001 tttatatgat tattctccta tcgcttcatt ggagaataag gagcatataa taaaacacga
     3061 agcaaagatt ggcagtcaat gggtaaacct tccttataaa acaactggta gttctatcat
     3121 atatagagta actaatgaga agagccaagt aatcacactt cctgtatatg cttatttagg
     3181 aacgaatgta gaaataaatc atcaacctgt aaaggagcag gtgaatgcta atggattggt
     3241 acaaataagc ggagtcaaag gagttgctga tattaaagta agcacaagtt atacagtaac
     3301 gactattatt tcttatcttg ttacattaac tgcatattta tgtttatttt ttaaaatagt
     3361 tttccataaa aggtaaatta acttttcatt tataaaataa attgattaaa aaggagaatc
     3421 tgtcgataag gattctctct tttttatacg aaaaatctgt agggggatga agtaatcgct
     3481 tatttcctca ctttatgata aattacagct aaagtcacta gcttgtcttc ttcggaaagg
     3541 aggagaatct atggatgagg aacaaatatt cattcaaaca ctttataatc tgattttgaa
     3601 tccgaataca cgagattggg aacgaaaagt attgattcag acaaagaatg acataagaga
     3661 aaatatatcc gttaaagaac agttatcaaa actagaagca acactacgtc cattagctat
     3721 ccgaatgaat ctgacgcctg atgtaatgga cttttattta ttgattacgg aaggatttga
     3781 taaggagcaa aaatatgact tttccaagca tgccatgcaa gatgctgact atcaagaacg
     3841 tgcagtattt gcagggggat gtttctggtg tatggtcgag ccgtttgaat cgaaaaaagg
     3901 gatcctatct gttttgtcag gatacacggg cggacatgtt gaaaaaccaa attatgatca
     3961 agtaagtggt ggttacacag gtcatgtgga agctgtggag atcatttatg acacaagaga
     4021 aataagttat agcgagttgc tgacaatcta ttggcaaatt actgatccca cagatacatt
     4081 tggacaattt caagatagag ggaagcaata tcgtccagtg attttttatc aagtcgaacg
     4141 acaaaaagaa ttagcggaac aatcaaaaca gaaactagac agttcaggaa ctttccacca
     4201 accaatcgtc acaaaaatcg aaccagccgg tacgttttgg ccagcagaaa actatcatca
     4261 gcaattttac aaaaaacaac cgaaacgtta taaaaaaatc cagcaagcac gtaatcaatt
     4321 tctgatctat caacgagtaa aaaacaaatg gcaaaaaaat atacgaaaa
//
LOCUS       DAYYJC010000148         4348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000148 DAYYJC010000000
VERSION     DAYYJC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.152"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(387..1853)
                     /locus_tag="ACHA9N_002659"
     CDS             complement(387..1853)
                     /locus_tag="ACHA9N_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9086073.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /locus_tag="ACHA9N_002660"
     CDS             complement(2144..4228)
                     /locus_tag="ACHA9N_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9086074.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1415 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaattt
//
LOCUS       DAYYJC010000149         4153 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000149 DAYYJC010000000
VERSION     DAYYJC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.154"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACHA9N_002661"
     CDS             <1..106
                     /locus_tag="ACHA9N_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9086075.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACHA9N_002662"
     CDS             complement(359..508)
                     /locus_tag="ACHA9N_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086076.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACHA9N_002663"
     CDS             970..1182
                     /locus_tag="ACHA9N_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086077.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACHA9N_002664"
     CDS             complement(1460..3259)
                     /locus_tag="ACHA9N_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9086078.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACHA9N_002665"
     CDS             complement(3383..3979)
                     /locus_tag="ACHA9N_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9086079.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DAYYJC010000150         4063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000150 DAYYJC010000000
VERSION     DAYYJC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.155"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..628)
                     /locus_tag="ACHA9N_002666"
     CDS             complement(86..628)
                     /locus_tag="ACHA9N_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9086080.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            763..1152
                     /locus_tag="ACHA9N_002667"
     CDS             763..1152
                     /locus_tag="ACHA9N_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086081.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1156..1920
                     /locus_tag="ACHA9N_002668"
     CDS             1156..1920
                     /locus_tag="ACHA9N_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9086082.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2049..2996
                     /locus_tag="ACHA9N_002669"
     CDS             2049..2996
                     /locus_tag="ACHA9N_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9086083.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3126..3866
                     /locus_tag="ACHA9N_002670"
     CDS             3126..3866
                     /locus_tag="ACHA9N_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086084.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1355 a          688 c          817 g         1203 t
ORIGIN      
        1 gatgtttttt aatcaaaata ataaataaaa actgtctaac atttaaaatt taatttagat
       61 attagacagt ttttaagttt gtgagttatt ttccttgtcg ttttctcact tttttagtcc
      121 agaaaccacc tgaaatatat tttggctcat aagaaatgat aaaagcacgc tcttcgattt
      181 ctttgatcag gcgatacagt tcacgttcgc tttttctagg agaaagtatt tcgagaacta
      241 gccgctcacc ttccagcccc attgcagaag tctgtgtcac accataaccg tgttctctca
      301 gtatacccgg caaactttta tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga
      361 tgtatcctaa tgccaatttg tcttcaatca aaattccgac actgatacct actgcgtaac
      421 ctaacgcata gacaaataaa ttcagtggat tatctaaacg atctaaaaca agggataagc
      481 cgacgatata aatcgtgatc tcgaccatac tgacaagtgg agcaatcagt cgatagcctt
      541 tcattgtcaa catgaaacgt aacgtgttca gagtgatata agcaaaatta atgaggaaga
      601 tcatccccaa catttttgga tcaatcattt gtaaaactcc tttcatttat ataacaacaa
      661 tatcaaaaaa aatcgtaagt gaagaaaaat tgaaacttta tacatacaaa aaaatgataa
      721 aatgattata atagaattat aaagataggt taggtgatta aaatgaaaac ggtcaatcgt
      781 gtgttttgtg ccagccacgt gctaagtctc cttctcagta tatacttagg tgtctattcg
      841 attcaacagc caattgaggt agagaacatt gtttttcaga tgtcgctgac agatggttta
      901 attttaagcg tttttttctt tttgatactg tttattggta atattttcgg tagcgttgcc
      961 agttttttga atttttctat ttatccactc ctttcgctag cattaggagt ggtgggactt
     1021 tgttctctgt gcttatttcc cgagccattc agtcctgcat ttatattatt tggtacactg
     1081 aacctgttcc agacaactgt tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa
     1141 ataggtgagt aacaattgaa tccaattata ttgaaacgtt ctgtccatca tattccagtg
     1201 ttagaagttg ttctgaaaga attaagagag gaacgtattc ccgttgtcat ctattatcac
     1261 ggatggcaga caagtaagga gttagttttg acgcaaggaa gaaaaattgc gcagcagggc
     1321 attcgtgtga tcctgccaga tgcgatgaat catggggagc gcaaacagcc ggtttcttct
     1381 attccatcct atacattttg ggatagtatc tacggaaatc tgtttgaatt tgacacactt
     1441 atcaagcatt tagaaaaaag aggtcttcta catgatcagc ttgcagttgg cggggtttcg
     1501 atggggggaa tgacaacatg cgccttgttg gctaaacatc ctcatatagt agcgggcgtg
     1561 tgtttgatgg gttcccctgc acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg
     1621 gaatataatt atgtcgtacc aaaagactat tttgatttga tttcatgggt taagagctat
     1681 gatttgtcat tgcagccgga aaagttagct ggacgtcctc tgttcttttg gcatggacaa
     1741 gctgacgaaa aaatccctta tatacaaacg gaacgatttg tagaaaaaaa tcggcataag
     1801 tcttacggaa aagaaattgt ttttcgttcc tcaaaaacac agcgacatat ggtacaaata
     1861 ccactaatgg aagaagcttc cactttttta gcaaaaaatt tgataaaaaa gtataaataa
     1921 ttgaaacgga aaatcactca tgacgataga ggattttccg ttttttattt tgggctataa
     1981 taaactctgt taggaaaatt tggtataatt gatagtaaga atgacaacat gttagaaagg
     2041 aagagagcat gcccacttta gcagatgttg caaaacgtgc caatgtttca aaaatgacgg
     2101 tctctcgagt catcaatcat ccggaacagg tgactgacga attgaaagag ttagttttct
     2161 cagcaatggc tgaactggat taccgtccaa atattgctgc aaaagcactt gtcacgaatc
     2221 ggtcgcagat catcaaatta tttatactgg aagaaatcga cacaactgag ccctattaca
     2281 tgaatttgct gatgggaatc gctaaacgga tcggtaaagc gcattattct ctccaattag
     2341 ttacaaatga tgccttcgac gtcggttctt gtgatggata catcatcaca ggtgtcagaa
     2401 aaaaagactt cgattggatc gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt
     2461 ctgaaggata tgattatgta gacagcgata atgaatatgg tacagcaaaa gcaacgcaat
     2521 atgccttaga aagagattat caaacgatta tttatatcgg tatcgaagtg gacgagcctt
     2581 ttgaaatttc aagggaaact ggttatttat ctgtgatgaa ggaagttgat cttcctagta
     2641 aaatcttgcg gtttccaaat cattcgacag aagtagaaag atatatgaca gaaaactggg
     2701 cagattttac agataaaact tgttttgtat gtagttcaga tcggctagca ctaggagcag
     2761 taagagggat tataaatgct ggaggacact tgcccgaaga atttggtgtg attggatttg
     2821 atggggtatt tttagaccaa atcagttcac ctaaattgac aacgatcaag caagatatta
     2881 tacgtatggg tgaagtatgt ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga
     2941 gtcaaggtta tcgtcgtttt tttcctgaac tagttatcag agagacaacg aattaagtct
     3001 tattactata tatatattta tgaaattcat cgctattttc ttaaaataat cagcgcattg
     3061 ctactgaaaa caatgaatct gtgctattat tgagaaaagt tccatttttg agaaggatgg
     3121 tttgaatgaa agaaatcaga cgaaaacatt tccgttttcc tgcgtacgat gatgagttag
     3181 gcgtaaaatt aaccaacgac aagaagcgag tattatttga cggacaagaa gatcttttga
     3241 ctgaacagaa tcagtccccg tttgaacaga tgaaagaatt tcatttcacg aacacgaaca
     3301 ccgagaaaat ccgtcgcaag cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg
     3361 aaaatttgga aaagcataaa ggaaaattac cggattatac cggccattac caatcagaaa
     3421 tcacatcgac aggacgaaaa aaactttttg gtgattcctc tcgttctgtc agacaaagtg
     3481 aaacaaggac agagtccaag catactacac gaaacgcatc tgttgacacg aaggtgcggg
     3541 agcgttctta ttttgttcct aaatatgtac ccgcatcgat tatcccagac cctagagaaa
     3601 gcaaaatttc tgaggatcat ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt
     3661 tgttcgatac agaaccgacg ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa
     3721 cattcaatcg aacaaaaacg ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa
     3781 aaagccatgg tatcttagaa cgctcattac aaggattgat cgatgatcaa ggaaactctt
     3841 tgggagaaaa tagctatttt aaataagtga tatacaaatt tatattcggc tacagttgga
     3901 aaggaaacgc tgcggcgttt ccttatttta tactctatcc gaaagtttat atcgtatcct
     3961 caaatttttt tgagtttaag cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa
     4021 taaaaaaaca aactattttc gcagatcgtt tgcaaaaata gtt
//
LOCUS       DAYYJC010000151         4043 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000151 DAYYJC010000000
VERSION     DAYYJC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.156"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..1658)
                     /locus_tag="ACHA9N_002671"
     CDS             complement(153..1658)
                     /locus_tag="ACHA9N_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9086085.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1655..2341)
                     /locus_tag="ACHA9N_002672"
     CDS             complement(1655..2341)
                     /locus_tag="ACHA9N_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9086086.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACHA9N_002673"
     CDS             complement(2534..3955)
                     /gene="gndA"
                     /locus_tag="ACHA9N_002673"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9086087.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1115 a          856 c          691 g         1381 t
ORIGIN      
        1 catctaaaag atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt
       61 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
      121 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      181 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      241 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      301 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      361 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      421 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      481 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      541 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      601 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      661 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      721 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      781 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      841 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      901 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      961 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
     1021 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1081 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1141 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1201 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1261 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1321 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1381 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1441 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1501 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1561 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1621 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1681 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1741 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1801 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1861 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1921 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1981 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     2041 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2101 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2161 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2221 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2281 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2341 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2401 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2461 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2521 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2581 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2641 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2701 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2761 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2821 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2881 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2941 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     3001 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3061 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3121 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3181 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3241 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3301 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3361 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3421 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3481 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3541 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3601 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3661 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3721 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3781 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3841 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3901 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3961 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     4021 tgcaaactaa agaacaacat att
//
LOCUS       DAYYJC010000152         3973 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000152 DAYYJC010000000
VERSION     DAYYJC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.157"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACHA9N_002674"
     CDS             complement(<1..283)
                     /locus_tag="ACHA9N_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9086088.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACHA9N_002675"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACHA9N_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACHA9N_002676"
     CDS             complement(2361..2723)
                     /locus_tag="ACHA9N_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086089.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACHA9N_002677"
     CDS             complement(2727..3035)
                     /locus_tag="ACHA9N_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086090.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACHA9N_002678"
     CDS             complement(3044..3463)
                     /locus_tag="ACHA9N_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086091.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACHA9N_002679"
     CDS             complement(3456..3689)
                     /locus_tag="ACHA9N_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086092.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>3973)
                     /locus_tag="ACHA9N_002680"
     CDS             complement(3686..>3973)
                     /locus_tag="ACHA9N_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9086093.1"
                     /translation="MVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNT
                     KKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1120 a          882 c          564 g         1407 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac cat
//
LOCUS       DAYYJC010000153         3965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000153 DAYYJC010000000
VERSION     DAYYJC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.158"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="ACHA9N_002681"
     CDS             complement(<1..1097)
                     /locus_tag="ACHA9N_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9086094.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="ACHA9N_002682"
     CDS             complement(1227..2603)
                     /locus_tag="ACHA9N_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9086095.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="ACHA9N_002683"
     CDS             complement(2603..3259)
                     /locus_tag="ACHA9N_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9086096.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>3965)
                     /locus_tag="ACHA9N_002684"
     CDS             complement(3269..>3965)
                     /locus_tag="ACHA9N_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9086097.1"
                     /translation="SGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKIL
                     MYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIML
                     GGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGN
                     VLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLM
                     LIKKPKEILSKIS"
BASE COUNT         1247 a          728 c          509 g         1481 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaa
//
LOCUS       DAYYJC010000154         3955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000154 DAYYJC010000000
VERSION     DAYYJC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.159"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1480
                     /locus_tag="ACHA9N_002685"
     CDS             <1..1480
                     /locus_tag="ACHA9N_002685"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9086098.1"
                     /translation="DRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIAT
                     IVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSK
                     KLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTP
                     HMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTT
                     YNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFL
                     DDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLL
                     EDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEE
                     NIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDI
                     KKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1470..2687
                     /locus_tag="ACHA9N_002686"
     CDS             1470..2687
                     /locus_tag="ACHA9N_002686"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9086099.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2700..>3955
                     /locus_tag="ACHA9N_002687"
     CDS             2700..>3955
                     /locus_tag="ACHA9N_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9086100.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1366 a          676 c          826 g         1087 t
ORIGIN      
        1 cgatcgattg ttggaacaag tggttctttt agcagatgaa tccttagagg aatatgacac
       61 agtaagcaaa caaacaatgt tgtatcgtga attactttca gatgaagaat caaaagaaga
      121 tctgattgcc acaatcgttg atattttggg atatgcaatt gcaccagagt atcttttcaa
      181 cgtcttagcg gatcaagcaa aacaagcaac gttccaattg aacgatctaa acaaagcctt
      241 tgtgcaattg gcatctacct acaatcaatt taatgggtta tttgatgatg tggatctcca
      301 atcaaaaaaa ctaggaacag atgagcaaca acgaaatgtg acgattaccg aagtcatcaa
      361 gaaattgaat gatgtagatg ttttagaaca cgatggggac gtgatcgggg atgcgtatga
      421 gtttctgatc agtcaatttg cttcagaagc agggaagaaa gccggagaat tttacacacc
      481 tcatatggtt tctgatatga tggcgcaaat cgtaacgctc gatcaaaagg aacgacgatt
      541 ctttagtgtt tttgatccaa cgatgggttc aggttcattg atgttaaacg tacgtaacta
      601 cttaacacac ccagataacg ttaaatatca tgggcaagaa ctaaatacaa cgacatacaa
      661 tctagcaaaa atgaacttga ttttacatgg tgtcgacgcc gaagaaatga atctccgcaa
      721 tggggatacg ttgaataagg actggccaac agatgaacct tacacatttg atgcagtagt
      781 aatgaaccca ccttattcgg ctaattggtc agcagatacg actttcttag atgattctcg
      841 gtttaatcgt tatgggaaat tagcgccaaa atccaaagca gattttgcgt ttcttcttca
      901 tgggttctat catttgaagg aaacaggtac catggccatc gtcttaccac acggagtttt
      961 attccgtggc gctgcagaag gggtcatccg tcaaaaatta ctagaagatg gcagtattta
     1021 tgctgtgatc ggtatgcctg cgaacttgtt ctttgggaca tcgattccaa cgacggtgat
     1081 tgtgttgaag aagaatcgtc aaactcgaga tgtattattc attgatgcta gtcgtgaatt
     1141 tgttaaaggg aaaaatcaaa ataaactatc ggaagaaaac attcaaaaaa tcctcgaaac
     1201 ctacgctgaa agaaaagatg tcgagaaata cgctcatttg gcaacctttg atgaaatcaa
     1261 agaaaatgac tataacttaa atatcccacg ctatgtagat actttcgaag aagaagaacc
     1321 aattgatatg gttcatgtcg gaaatgacat caagaagata cgacaagaac agcaagtgct
     1381 agaaaaaaaa cttttagaag ctatctcttc cctgcaaaca acacctgaaa atgaagcgtg
     1441 gttacaaggt gcgctggagg tgttcaaaca tgagcaatga tacacaacca gaaatccgtt
     1501 tcccaggatt tacagatgat tgggaacaga ggaagttggg ggacttcgtt gtagattatg
     1561 ttgaaaagac aagtgtacaa aatcaatttc ctatgctaac ttcatctcaa caaaaaggga
     1621 tagttttgca agaagactat tttgcaaatc ggcaagtaac tacagaaaat aatattggtt
     1681 actttgtact acctagagga tatttcacgt tccgtagtcg aagtgataat gatgtgtttg
     1741 tgtttaaccg taatgacatt attgatagag ggattataag ctatttttat cctgtattta
     1801 ctttaaaatc tgctgattcc gacttcttct taagacgtat taacaatgga atccaacgac
     1861 aattatctat tcaagcagag ggaacaggtc aacatgttct gtctctaaaa aagtttaaaa
     1921 atatagtagc aatgtttcct agtgaggaag aacaacaaaa aatcggcacc ttcttcaaac
     1981 aacttgacga cacgatcgct cttcatcagc gtaagttaga tttgctgaag gaaacgaaaa
     2041 aaggattctt acaaaaaatg ttccctgaaa acggagcaaa agtgccagaa atccggtttc
     2101 ctggttttac ggaagattgg gaagagcgta agttgggaga attaggtaaa actcaatcgg
     2161 gaattggatt tcctgatgcc gagcaaggtg gttctgaagg tactccattt tttaaagttt
     2221 ccgacatgaa taacattggt aatgagtatg aaatgagaaa tgcaaatcat tatgtatcta
     2281 atgagcaaat tgagcgtaaa aaatggaagc caataaaaga tgtgccagcg attatatttg
     2341 ctaaagttgg cgcagcaata atgcttaata gaaaacgttt ggtcacaagc cctttcttaa
     2401 tagataacaa taccatggct tatttattta ataatacttg ggatatctat tttggtaaaa
     2461 tattgtttga aactataaat ttgccaagat actcccaagt aggggcattg ccttcttata
     2521 acagttctga tattgagaat atcagtgtta aggttcctgt aaaggatgaa caacaaaaaa
     2581 tcggtacttt cttcaaacag ctagatgata cgatcgctct tcatcaacgt aagttagatt
     2641 tactaaaaga aacgaaaaaa agcttcttac aaaagatgtt tgtctaggag ggaagaagaa
     2701 tgaaaattag tcaccgtgag gaagcagaag tcgaagaaca gttgattcgt gtacttggag
     2761 aagggcataa tcagtggacg tatcgtccgg atctaaaatc agaagaagac ctttgggtca
     2821 atttacgcca aaaaattatc tcaaataatc aagcagaact gaatgatttt cctttgaccg
     2881 ataaagaatt tgaaacgatc aaaacggaat tactgctgcg aacgaaaacc ccatttgatg
     2941 ctgcgaaatg gttaaaaggt gaaaatggga tggctcggat cacgattgaa cgggaagatt
     3001 cgcaattggg ttctgtctca cttatccttt attccaatca agacattggt ggaggaatct
     3061 ctacttatga agtggtccat caaattgcaa aacgtggaag taacatagaa gcacgggatc
     3121 gacgttttga cgtcacctta ttaatcaatg gattaccgat tgtccaaatc gagctgaaac
     3181 aagtaactgc aaaagatggt gtttatcagg catttaacca aatcaaaaaa tatgcagaag
     3241 aaggtatgtt tcggaacaat atcttttcga ccttacagtt gtttgtggtt tccaatgaac
     3301 aaaccactcg gtattttgca aatgccttac cgaaagactt gcaccctaag tttttattta
     3361 gttggcgaac gaaagacaat gaaaaagtag aaaacttata tgagttttgt aaacaagtgt
     3421 taaatatccc ggatgctcat cgtttgattg cggattatac aatcgtcagt gaagatcaag
     3481 acaataaaac cctcatggtg ttacatcctt atcaagtaca tgcgattcaa gccttgttta
     3541 ttgccgcaaa taaacatcaa tcagggtatg tgtggcatgc gactggttca ggaaagacgt
     3601 tgactagttt tgtatctact aagcttttag cacgaaaatc ggggatcgat cgaacgatca
     3661 tgttagtgga ccgcaaagat ttagataatc aaaccaccac agagtttaca aaatttgctt
     3721 cggaatttaa tactgggatt tcttccggga acgccaaagc gaatagcttg atcgttggaa
     3781 cgggaagtgc caaagaatta agtgaaaccc tgttggcaga tgccaatgca aatgttgtaa
     3841 tcattacaac ccgtcaaaaa ctagatgcgg cgttgaaata tgcaaagaaa caagaagaga
     3901 aaaaaggaac caatcgcttc caaaaattaa tgggccaaca tatcgtgttt gtagt
//
LOCUS       DAYYJC010000155         3943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000155 DAYYJC010000000
VERSION     DAYYJC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.160"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(591..1148)
                     /locus_tag="ACHA9N_002688"
     CDS             complement(591..1148)
                     /locus_tag="ACHA9N_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086101.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1327..1938)
                     /locus_tag="ACHA9N_002689"
     CDS             complement(1327..1938)
                     /locus_tag="ACHA9N_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086102.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2051..2308)
                     /locus_tag="ACHA9N_002690"
     CDS             complement(2051..2308)
                     /locus_tag="ACHA9N_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086103.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2905..3183)
                     /locus_tag="ACHA9N_002691"
     CDS             complement(2905..3183)
                     /locus_tag="ACHA9N_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086104.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3217..3468)
                     /locus_tag="ACHA9N_002692"
     CDS             complement(3217..3468)
                     /locus_tag="ACHA9N_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086105.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1196 a          808 c          523 g         1416 t
ORIGIN      
        1 gcttctgtcc cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga
       61 ttatctcatt tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt
      121 tttgacttgc gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt
      181 aacgagtaat atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc
      241 ccgatttcaa ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg
      301 gtcatttgtt gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc
      361 tcgtctacca aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg
      421 attcgtataa agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt
      481 cagattcatt tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat
      541 tgttccatta caacattcac tggctgatga aatggattag tagatttcga ttattcaaag
      601 cgattttcta ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac
      661 agatcttcta aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat
      721 tctattgtta cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca
      781 tctcccatag aatagttagc actttctggg acataatata ttcttcgacc atcctctaat
      841 tcatctaata agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga
      901 taccatgcca tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct
      961 tctatcacaa tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg
     1021 gtaatttctg gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt
     1081 cccagatgaa acacagctat aacaacaata atgattaata ttttgtttct atttgataaa
     1141 tttctcatta ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta
     1201 atatcaaaat atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag
     1261 aaaggccaaa tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat
     1321 tgttttctaa tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt
     1381 aattccgtat tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta
     1441 tccactttct cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag
     1501 ggctatatgt ggttttaatg acattgattg tacattttcc cccatataat attgcgcact
     1561 tcctcgacta aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc
     1621 taatctactt ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt
     1681 aaactcagct atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat
     1741 cctaaatgga aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc
     1801 aaagaaagga aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc
     1861 cgatgtaatc caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt
     1921 ccccctatta ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc
     1981 ttccatacct tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc
     2041 atcatattgt ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa
     2101 catttaatgc atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt
     2161 ctaattcata agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta
     2221 tagctactga atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa
     2281 tagtaggatt tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac
     2341 ttttttgaac tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc
     2401 cactaaggaa tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt
     2461 ttaaatcatt atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa
     2521 aatgctttat caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc
     2581 gatttttcct atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg
     2641 gaatggtaat tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa
     2701 taccgagatg atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa
     2761 tgctctatcg catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt
     2821 tatgctgttc ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat
     2881 tattcgtgtt gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct
     2941 gtctttttgc agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt
     3001 taacttctta ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg
     3061 atcttttatt ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc
     3121 atcagtgctg ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc
     3181 catttcttcc gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc
     3241 atggattgga cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa
     3301 catccctaag gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac
     3361 cgtttgtaat aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa
     3421 cattgaattt ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc
     3481 tactgttctg ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg
     3541 aatacgactt gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt
     3601 aatctctatt catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga
     3661 tagtggaaga attatttagc caggatagac actatttgtg aaacacatgt ctttactttt
     3721 gaaattctat gatgaaaagc cttggaatac agtagctctt aattggtaat actttttcca
     3781 ttataaataa cctcttttca ttttatattt atcccctcta aaaataatat ctaatctaat
     3841 attttaaact tatatttccc tcgctgtgct aatagagcat tactaaataa atagaaacta
     3901 gaaaaaagca ttacgcctgc cagaataaac caattatttg tat
//
LOCUS       DAYYJC010000156         3872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000156 DAYYJC010000000
VERSION     DAYYJC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.161"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..2254
                     /locus_tag="ACHA9N_002693"
     CDS             155..2254
                     /locus_tag="ACHA9N_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9086106.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2450..3313
                     /locus_tag="ACHA9N_002694"
     CDS             2450..3313
                     /locus_tag="ACHA9N_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9086107.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3418..>3872
                     /locus_tag="ACHA9N_002695"
     CDS             3418..>3872
                     /locus_tag="ACHA9N_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9086108.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1542 a          645 c          781 g          904 t
ORIGIN      
        1 ttgagactac gcagtctcaa gctttttttt ctataggaag gaaattcatt cattattttt
       61 aagtaaatcc taaaaataag ctattggcgt gacgtataga atatgataga ttaaatggta
      121 gcgtaaacct agtaaaggag ccgatcgcta agtgatgaaa aaatgtccaa attgcggatc
      181 gaaaaaaatg gagcagaata ctatctgtcc agactgcggt catcaaataa ctgcagaaga
      241 tcagcaaata aaagaatcaa ttgaagaaac aaatacagat gcagctatgc aggataaatc
      301 aactgaagat gcagtctttc ctgatacaga attaaacgat ccaatcgaat ggtcggaatt
      361 gaaagattta cctctagaat ctgtaatgga attattcgaa aaagcagaac cagaagacga
      421 agaaccacct gctcacgatc aagtaactaa tatagagaaa aatgaacaga agaaagcaac
      481 tgttcatgag acttcggaag aatcaggaaa gaatcaagaa gaaacgcttg aatcaaaaca
      541 aaaaagaaga gtagctgaac tgaaagaaat cgtcgataat gaggaagaaa attcgatact
      601 atccgcttat atcaaagcac atcgtgaaga tacaaaagaa gagcacgcga aagagctgct
      661 caaaatgatc agtgaaaaaa ttgcacagga aaacaaagta gagccagaaa aggaacaaga
      721 aactgaaaaa gactctgata gagaaaacgc cacaggtaaa gaaacagtaa aaataaaaga
      781 cgtaaaaacg gatatcacag aaaaaactga agtatttact gaaaaacaat tagaaaagga
      841 aaaaacgaat aaactgccgg aagcagagaa aaaagaaata ctcgaaaaca cagacaaaaa
      901 gtcaacagag cccgatgaaa taaatagtga atccgatgct gaaaatcaat ctgtaagcaa
      961 agagggtact gatcttgaag caaaaataga aaaagaagag aaccagccta agctgtttga
     1021 agcagaagcg aaatcaataa caatcgtgcc agatactgaa aaaacacatc aagaatcaaa
     1081 cttagaaaaa acagatccag ataaagcgca aaagaaaaaa tcaaaaaaag taccttatct
     1141 cgttttagca gcagtacttc tactcggtgg aggaagctgg gcttattatg accatcatca
     1201 aaaagtagaa gccgaagtag cagcagaagc aaaaagacag caacaaatgg aagaacttca
     1261 tgagaaactt gctgcctttt atacagatga tcaccatcaa tttattcgtg cagatatgat
     1321 tcatcaagat ttatcaaaac taaaaaatga tttatccagt cttaagaatg aaaaaggata
     1381 ttcagagtta gaaacaactt accaagatat ccaatctaag atccgagata tccaaaaagt
     1441 gaatgagtgg ttcatagcac ctgccatcgt cgatgatcat ctagcagatg acatcaagtt
     1501 gaaagcagat aaagagatcc aagcaatcga aacggataac acagattttg gaaaattgat
     1561 tcaacaagca caaacagaag ctaaaaatca atatgctgaa ttgcaaacag ctaaagaaaa
     1621 aacagctata gtctttaaag acggaaaagt agtggattca gcaacacgcg accaatacaa
     1681 gacggcaaaa gaagcagtag aaaaagtgaa aaatacagat ctatctgctc agttgaatga
     1741 acaattgaaa caagtagata agatgctcac tgataaagag aaaaaagaag aagaggaaaa
     1801 gaaaaaagca gaagaagcga aaaaagcagc tgaagaacaa gcaaaacaag ccgctcaagc
     1861 agcagctgca gctcaagccg ctcaacaagc tgcggtcgca caagcagcac agcaagaagc
     1921 ggcatcctcg accccttcct cttcaaactc agcgaatcag ccaatcaaat ctgccagagc
     1981 aagtgatatt gccgatgcat ctaatccagc atggacgtgg gcaccaggtg tccaaagcaa
     2041 agtgattgct acatgtatcc aacgtggata tatcgttgaa ggaggttaca gattagaaaa
     2101 agccagaatc gaaaatgggg aaggctatta taatctatat gccacttcaa caaaatctgc
     2161 attgatgaaa ggcattggag aaagtgcttt gccattttac atcgtaacca tcaactgtaa
     2221 aactggatgg ttcggaggaa atgggagcaa ttaattttag gcaaatgaaa gaggctgtga
     2281 gcctgccgtt taacaccgtt tattcgaaaa aggggagtat gacacaactg ttgtcatact
     2341 ccttttttca cctttccaaa ccaaggctca gctattcaag tagataacat tatgttatga
     2401 tagcaaagaa atgtattaag aaaacctttc actatagagg gagaaaaaaa tgaacgattt
     2461 attagcaact atatttacag gtattattat tgatgaaaat gaaaaaaact actttgtcca
     2521 aaaaaacgga caaacctttg gcttaagcaa atcagaaggt gaacacaaaa tcggtgaagc
     2581 agttgaagga ttcggttacc aaaatcaaaa acatgaaaat cggatcacta cacaaattcc
     2641 taaaagccgt atcggtcatt acgcttttgg aatcgttact gctacacgaa aagatttagg
     2701 tgcgtttgtc gatatcggtc ttccagataa agactttgtc gtatctcttg atgaattgcc
     2761 gacaatgaca gagctatggc ctaaaaaagg ggatcgcttg atgatttccc ttagagttga
     2821 tgcaaaagac cgtatttggg gcagtttagc agatgaaaaa atattcaaat ctttaagcaa
     2881 atatggaaca gaagaaatga aaaacaaaga tgtgatagga acagcatacc gcttgaaatt
     2941 aacaggtacg tacgttttaa cggaagattt ctatcttggg tttatccatc cttctgaacg
     3001 atataatgaa cctcgtctag gcgagcagat aaaaggtcga gtgattggtg tccgaccaga
     3061 cggtgtactg aatctttcat tgaagccaag agcatatgaa gctatatcag atgatgcaca
     3121 aatgatttta accattttag aacgttctgc agatcagcaa atcaacttta cagataaatc
     3181 ggatccagaa gaaatcatga aagcttttgg tatcagcaaa ggagcattta agcgcgcatt
     3241 aggtaatctt ttgaaacaag gattgattac acaagaaaac ggtgtgacaa aactagtcaa
     3301 aaaatctaat tgagaatccc ttgcatgttt ttcttaacta gcttataata attttaatta
     3361 acatagtgtt atatcttttt ttatttgtaa ttattataaa ttgttggggt tgagtaaatg
     3421 gtcatgaatg ctatttcggc gatgaaaaaa acaaaacagc agctacatga agcggggttt
     3481 aagctgacac cgcaaagaga agctactctg cttgtgcttt tggaaaatga gaaagagcat
     3541 ttatctgcag aagaaatctt ctttttagtg aaaaaaaaga gtccggaaat tggacttgct
     3601 actgtttatc gaacccttga aatcttgaca gaattgaaaa ttgtcgataa agtaagtttt
     3661 aatgatggat tagcccgtta tgatttgaga aaagaaggcg ccaaacattt ccatcatcat
     3721 ttactatgct tgaactgcgg cacgatcgag gaagtagaag aggatctttt acttgaagtg
     3781 gagcaagtag tagaagaacg ttatcatttt ttagtaaaag atcatcggct aacgttccat
     3841 ggaatctgtc aggaatgcta tgaaaaagaa aa
//
LOCUS       DAYYJC010000157         3712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000157 DAYYJC010000000
VERSION     DAYYJC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.163"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHA9N_002696"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHA9N_002696"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9086109.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHA9N_002697"
     CDS             complement(2260..2511)
                     /locus_tag="ACHA9N_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9086110.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHA9N_002698"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHA9N_002698"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9086111.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHA9N_002699"
     CDS             3461..3679
                     /locus_tag="ACHA9N_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086112.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DAYYJC010000158         3695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000158 DAYYJC010000000
VERSION     DAYYJC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.164"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACHA9N_002700"
     CDS             75..545
                     /locus_tag="ACHA9N_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9086113.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACHA9N_002701"
     CDS             complement(465..683)
                     /locus_tag="ACHA9N_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086114.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACHA9N_002702"
     CDS             790..1104
                     /locus_tag="ACHA9N_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9086115.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACHA9N_002703"
     CDS             1208..2386
                     /locus_tag="ACHA9N_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9086116.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACHA9N_002704"
     CDS             complement(2567..2752)
                     /locus_tag="ACHA9N_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086117.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACHA9N_002705"
     CDS             2789..3082
                     /locus_tag="ACHA9N_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9086118.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACHA9N_002706"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACHA9N_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1261 a          546 c          716 g         1172 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatt
//
LOCUS       DAYYJC010000159         3577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000159 DAYYJC010000000
VERSION     DAYYJC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.165"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..878)
                     /locus_tag="ACHA9N_002707"
     CDS             complement(27..878)
                     /locus_tag="ACHA9N_002707"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9086119.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            1001..1468
                     /locus_tag="ACHA9N_002708"
     CDS             1001..1468
                     /locus_tag="ACHA9N_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9086120.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(1731..2213)
                     /locus_tag="ACHA9N_002709"
     CDS             complement(1731..2213)
                     /locus_tag="ACHA9N_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9086121.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2313..3176)
                     /locus_tag="ACHA9N_002710"
     CDS             complement(2313..3176)
                     /locus_tag="ACHA9N_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9086122.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         1039 a          771 c          565 g         1202 t
ORIGIN      
        1 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
       61 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
      121 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
      181 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
      241 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
      301 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
      361 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
      421 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
      481 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
      541 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
      601 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
      661 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
      721 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
      781 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
      841 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
      901 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
      961 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     1021 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     1081 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     1141 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     1201 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     1261 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     1321 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     1381 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     1441 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     1501 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     1561 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     1621 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     1681 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     1741 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     1801 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     1861 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     1921 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     1981 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     2041 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     2101 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     2161 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     2221 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     2281 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     2341 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     2401 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     2461 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     2521 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     2581 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     2641 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     2701 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     2761 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     2821 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     2881 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     2941 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     3001 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     3061 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     3121 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     3181 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     3241 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     3301 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     3361 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     3421 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     3481 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     3541 tatttctaac aaaatcactt catttttgtc agataaa
//
LOCUS       DAYYJC010000160         3478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000160 DAYYJC010000000
VERSION     DAYYJC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.166"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHA9N_002711"
     CDS             complement(<1..2232)
                     /locus_tag="ACHA9N_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9086123.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHA9N_002712"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHA9N_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          978 a          770 c          548 g         1182 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataata
//
LOCUS       DAYYJC010000161         3344 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000161 DAYYJC010000000
VERSION     DAYYJC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.167"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACHA9N_002713"
     CDS             278..787
                     /locus_tag="ACHA9N_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9086124.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACHA9N_002714"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACHA9N_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086125.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACHA9N_002715"
     CDS             2322..3302
                     /locus_tag="ACHA9N_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9086126.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
BASE COUNT         1195 a          535 c          658 g          956 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat attt
//
LOCUS       DAYYJC010000162         3128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000162 DAYYJC010000000
VERSION     DAYYJC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.168"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACHA9N_002716"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACHA9N_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACHA9N_002717"
     CDS             1360..1992
                     /locus_tag="ACHA9N_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9086127.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACHA9N_002718"
     CDS             2200..2424
                     /locus_tag="ACHA9N_002718"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFM9086128.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACHA9N_002719"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACHA9N_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9086129.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACHA9N_002720"
     CDS             2749..3018
                     /locus_tag="ACHA9N_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9086130.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAYYJC010000163         3124 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000163 DAYYJC010000000
VERSION     DAYYJC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.169"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..909
                     /locus_tag="ACHA9N_002721"
     CDS             <1..909
                     /locus_tag="ACHA9N_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086131.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
     gene            923..1072
                     /locus_tag="ACHA9N_002722"
     CDS             923..1072
                     /locus_tag="ACHA9N_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086132.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ACHA9N_002723"
     CDS             1069..1515
                     /locus_tag="ACHA9N_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086133.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ACHA9N_002724"
     CDS             1517..1654
                     /locus_tag="ACHA9N_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9086134.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ACHA9N_002725"
     CDS             1691..1984
                     /locus_tag="ACHA9N_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086135.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ACHA9N_002726"
     CDS             1981..2205
                     /locus_tag="ACHA9N_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9086136.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..>3124
                     /locus_tag="ACHA9N_002727"
     CDS             2202..>3124
                     /locus_tag="ACHA9N_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9086137.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYV"
BASE COUNT         1091 a          548 c          655 g          830 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgt
//
LOCUS       DAYYJC010000164         3064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000164 DAYYJC010000000
VERSION     DAYYJC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.170"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(330..692)
                     /locus_tag="ACHA9N_002728"
     CDS             complement(330..692)
                     /locus_tag="ACHA9N_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086138.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="ACHA9N_002729"
     CDS             complement(766..1146)
                     /locus_tag="ACHA9N_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9086139.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1228..2403
                     /locus_tag="ACHA9N_002730"
     CDS             1228..2403
                     /locus_tag="ACHA9N_002730"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9086140.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2480..>3064)
                     /locus_tag="ACHA9N_002731"
     CDS             complement(2480..>3064)
                     /locus_tag="ACHA9N_002731"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9086141.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          955 a          586 c          503 g         1020 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccat
//
LOCUS       DAYYJC010000165         3055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000165 DAYYJC010000000
VERSION     DAYYJC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.171"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..707
                     /locus_tag="ACHA9N_002732"
     CDS             <1..707
                     /locus_tag="ACHA9N_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HFM9086142.1"
                     /translation="YLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYMETG
                     KIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSHIDI
                     DYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGLMAI
                     RFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSELAKK
                     VKVTFLTDPRWVRIDK"
     gene            836..1420
                     /locus_tag="ACHA9N_002733"
                     /pseudo
     CDS             836..1420
                     /locus_tag="ACHA9N_002733"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            1366..1884
                     /locus_tag="ACHA9N_002734"
     CDS             1366..1884
                     /locus_tag="ACHA9N_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086143.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            1907..2932
                     /locus_tag="ACHA9N_002735"
     CDS             1907..2932
                     /locus_tag="ACHA9N_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9086144.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
BASE COUNT         1093 a          320 c          624 g         1018 t
ORIGIN      
        1 gctatctgtc atataagaat actttaacaa ttgaagcaat gaatgaaata ttcgaaatga
       61 tggatgaaga aaaaaaggta tcttatgata aacaatatat agaagattta tactacaaat
      121 atatggaaac tgggaaaata caggaggaat atatggaaga actgagaaaa tttctctcag
      181 acaaaaaaat tctactgatt gcaccaggaa aaagttctgt agatgaggta gaaagaataa
      241 aagagtttgt taccaaagaa gaagtcgtaa taattagtgt aaattttgaa tattctcata
      301 tagatataga ttacgtattt ttaagtaatt taagacgttt tcatgaatta ccgaaagaaa
      361 gcagaactaa atgtattgtt acatcaaata tacaagcatc ggatgtatat ttacggacga
      421 gttataaaaa gttgttatct gaaagggaag ttgtaagaga caatgcagga ttaatggcaa
      481 ttagattttt agccgatatg aatgtggatg aaatattctt agctggattt gatggatatt
      541 cacataatga agatgagaat tatggtgagc aatccatgga aattataaca aaatttgcat
      601 tattagatgc aatgaacgtg caaatgagag atgaattgtc agaattagct aaaaaagtaa
      661 aggtgacatt tttaacggat ccacgttggg ttcgaattga taagtagtag taataatgtt
      721 tttaaaattt tgataaagag atagtgagag aataaaaatg ggtaaagtct taaataaaaa
      781 agaagaaaaa tatttaaggg acgatggggc gagatgaaac tctaattttg aacttttaag
      841 aatcatttct atattgtgta ttgttgctta ccatgctaat ttgcgtttaa attataatgc
      901 agacggaatt atagtacatg gttcattaac tgctaaccag attttcttga attatattgg
      961 tatttggggt cagctaggag ttaattgttt tatatttatt tcggcatatt ttttaagtga
     1021 gaaaagaaac attcactttg aaaatgtttt gggaattgta ttagaatgta ttttttatag
     1081 tgtattagtt tatggaattc tttgtgcatg tggaatagaa acattttcat tgaacagctt
     1141 tgtaaaagaa atattatcat ttgctctagg tagatattgg tttattacat cctatttagc
     1201 attgtatatt ctaagtccgt atttaaatat catgcttaac aatctggaaa aacttcagtt
     1261 gcaaaaaata agtatgttat tgtttatagg tgtatgctgt tatcgtatgt tatttaaaac
     1321 atctttgttt ggggattgtg ctgttttaat tgcactatat gtcatatgtt atacactgaa
     1381 aaaaacgata ataatatttt tgagagacac agaatcatag gaacaattgt atgtataaca
     1441 gttataccac ttctcatatc cataggaata cttttcaaat ttataccaaa tgcaatggtt
     1501 aaccagatgg ttcatattgg ttctccattg atggttatag caagtatatt tttattttat
     1561 gtatttaaaa atttttcatt ttacagtaag tatataaata aacttgcgag aaccagttta
     1621 ggggtatata ttttacatga aaatcattat ttcattgatg gtatgaagtc tagaggctta
     1681 atatgggatg ggatatttgg attaggcaaa aggtttttag atagggattt ttggatttgg
     1741 tttataattg cagttgtagt tgtgtgcata atttgcatgg ctatcgatgt tgtaagagaa
     1801 aaatttgaaa gacagatttt taaaattaag agtgtaaaga acctgtgtaa acttatgaat
     1861 catgttataa atatataccc ataattaata aatgaggaga aaagaagtga aagttatatc
     1921 attcggagaa ttattattga gacttgcagc acccggttat tcaaagttat ttcaaagaga
     1981 tagtttggag actacattct gcggagggga agctaatgta gcggtatcct tagcaatatt
     2041 tggatatcaa tcagaattta taacaaagct tccggattca gatgttggat atgcggcagc
     2101 taatgctttg cgttattttg gagtaaatgt tgataatatt atatttggag agggacgcat
     2161 gggactgtat tatttagaaa aaggagcagc tcagagacca tctaaagtaa tttatgacag
     2221 aaaattttct gcaatttcgt tagctgatag tgatgaattt aagtggaagg agatcctaaa
     2281 cgatgcaaat tggtttcatt ggacagggat aaatccagca ttgtctgaga atctctttga
     2341 gacgtcctta gatgcatgtc aggctgccaa aaaaagaggt ataacaattt cttgtgattt
     2401 aaattatcga tccaagcttt ggacatcaga ggaagcaaaa gaaaagatgg aacagatact
     2461 accattggtt gatatttgta tttgtaatga agaagatgca gaaaaagtgc taggaattaa
     2521 agctgagcat acaaatatta atagtggaga attaaataaa gatggctatg tgcaggtggc
     2581 tagggagatt cacaagaagt ttggatgtag gtatgtagct acaactctgc gtagaagtta
     2641 ttcggcaagt agaaatggtt ggagtgcaat gttatacgaa gcagaaaaag ataaagcgta
     2701 tttttccaca gaatacaaca tacaaattgt tgatcgcgtt ggtggtggag acagcttttc
     2761 tgctggtata atatatgcgt tatgtgaaaa tatgtccgga caaaaggcta ttgaatttgc
     2821 gactgcatct agttgtttaa aacatactat agaaggtgat tttaatcgaa ctacaattga
     2881 ggatgtatat aatttaatga atagcggtgg aaatggtcgc gtgttgcgtt agtcaacata
     2941 taaagataaa tcacagtaat ttttatggtg attatctgtc ttagtagtga ttagaataac
     3001 gtgaaaataa attgttgagc gtttatactg ttttgaaaaa taaatttaac atatt
//
LOCUS       DAYYJC010000166         3052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000166 DAYYJC010000000
VERSION     DAYYJC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.172"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACHA9N_002736"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACHA9N_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACHA9N_002737"
     CDS             complement(612..1160)
                     /locus_tag="ACHA9N_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086145.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACHA9N_002738"
     CDS             complement(1161..2018)
                     /locus_tag="ACHA9N_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9086146.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACHA9N_002739"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACHA9N_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACHA9N_002740"
     CDS             complement(2580..2909)
                     /locus_tag="ACHA9N_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9086147.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..>3052)
                     /locus_tag="ACHA9N_002741"
     CDS             complement(2986..>3052)
                     /locus_tag="ACHA9N_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9086148.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DAYYJC010000167         3038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000167 DAYYJC010000000
VERSION     DAYYJC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.173"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..869)
                     /locus_tag="ACHA9N_002742"
     CDS             complement(255..869)
                     /locus_tag="ACHA9N_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9086149.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1475..1852)
                     /locus_tag="ACHA9N_002743"
     CDS             complement(1475..1852)
                     /locus_tag="ACHA9N_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086150.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2152..3033)
                     /gene="galU"
                     /locus_tag="ACHA9N_002744"
     CDS             complement(2152..3033)
                     /gene="galU"
                     /locus_tag="ACHA9N_002744"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9086151.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          638 c          468 g         1014 t
ORIGIN      
        1 ctttttccac tactttttat ttttctgaca ctacatattc ttataactca tttctcataa
       61 ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa atcaaagatg
      121 atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca ttaataatcg
      181 ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa ggaactttgt
      241 caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc tgtaaaatac
      301 aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat cgctggatct
      361 agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa aaccaaatca
      421 gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg tgacaaatca
      481 aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc tagttcgatg
      541 attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga tttgacagaa
      601 agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc tccggctgga
      661 ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc aaatgctaca
      721 ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat ttgtttcgcg
      781 atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc ttccaattgg
      841 tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat acttgacatg
      901 aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta gacagtaaca
      961 catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc ccaatacctt
     1021 gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg gtagaatttt
     1081 caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg gcaaaatgat
     1141 cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa ctaagggtca
     1201 gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat ctcttatcca
     1261 agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat aaataacata
     1321 aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat tcttttatat
     1381 aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata gtttatccat
     1441 tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc gttcttctac
     1501 tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt ctgcctatcc
     1561 gagactttct tttcatatat gggatataaa tgattagata cagtacagca aaaaataaag
     1621 caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta gcaaccccaa
     1681 cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc atgcgaaaca
     1741 tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt ccttccatca
     1801 gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc atagtgaaca
     1861 cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat cttctggctt
     1921 ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa aattaaacca
     1981 ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc gttttccacg
     2041 actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac gcacaagctg
     2101 ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca gttaactttc
     2161 tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc caatttccga
     2221 atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc ctacgtcgta
     2281 tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag tctgaagtgc
     2341 atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata tctcaaatat
     2401 tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt ttcccatttc
     2461 aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg ttttaggatc
     2521 aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa cagcggaagc
     2581 ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat ctttcatcaa
     2641 atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg cttgaagcac
     2701 ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga cttgagtcgt
     2761 ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt tttccaattc
     2821 gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta cgatcaagat
     2881 gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg gtttatccac
     2941 gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg ttcctagccc
     3001 agcggcagga atgactgctt ttttgacctt cacttgct
//
LOCUS       DAYYJC010000168         3012 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000168 DAYYJC010000000
VERSION     DAYYJC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.174"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1077
                     /locus_tag="ACHA9N_002745"
     CDS             202..1077
                     /locus_tag="ACHA9N_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9086152.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ACHA9N_002746"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ACHA9N_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ACHA9N_002747"
     CDS             complement(1642..2973)
                     /locus_tag="ACHA9N_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9086153.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1060 a          544 c          466 g          942 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tt
//
LOCUS       DAYYJC010000169         2974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000169 DAYYJC010000000
VERSION     DAYYJC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.175"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACHA9N_002748"
     CDS             complement(37..1257)
                     /locus_tag="ACHA9N_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9086154.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACHA9N_002749"
     CDS             complement(1277..1888)
                     /locus_tag="ACHA9N_002749"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9086155.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACHA9N_002750"
     CDS             complement(1931..2863)
                     /locus_tag="ACHA9N_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9086156.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          813 a          623 c          516 g         1022 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attt
//
LOCUS       DAYYJC010000170         2887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000170 DAYYJC010000000
VERSION     DAYYJC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.176"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            799..1053
                     /locus_tag="ACHA9N_002751"
                     /pseudo
     CDS             799..1053
                     /locus_tag="ACHA9N_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="ACHA9N_002752"
     CDS             1123..1635
                     /locus_tag="ACHA9N_002752"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086157.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="ACHA9N_002753"
     CDS             1632..2669
                     /locus_tag="ACHA9N_002753"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086158.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1042 a          513 c          574 g          758 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacaccga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgcactg
     2881 cctctac
//
LOCUS       DAYYJC010000171         2859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000171 DAYYJC010000000
VERSION     DAYYJC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.177"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..509
                     /locus_tag="ACHA9N_002754"
     CDS             159..509
                     /locus_tag="ACHA9N_002754"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9086159.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            923..1741
                     /locus_tag="ACHA9N_002755"
     CDS             923..1741
                     /locus_tag="ACHA9N_002755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9086160.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1814..2173)
                     /locus_tag="ACHA9N_002756"
     CDS             complement(1814..2173)
                     /locus_tag="ACHA9N_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9086161.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          972 a          403 c          509 g          975 t
ORIGIN      
        1 tgcttcgcaa cgatggcaac cggagtttct tgatgaaaat atgcttctat cattacaagc
       61 tcgtttggtg taagatgggt ataagtcatt tatgttcact ctccttgtat gctttagcgg
      121 gtattacaat ttgagtgtaa cataaatggc ttttttattt gtctcgctta attatacaaa
      181 cggcgtttta taaagcaatc attcctgaga acttagatga ggttaaactt tatttatggg
      241 aagataacgg agaaattgtc ggttttagtg gtgtaagtgc agaagaatta gttatgctat
      301 ttcttgatcc agattttatt ggtaagcgat atggtagtca aatattaatt gagctgattg
      361 aaactgaaga tataaaaaga attgatgtta atagtcaaaa taaatatgca aaacattttt
      421 atttaagcca tggatttgaa attgaatctg aagaagactt agatggtttt ggtaaaccat
      481 accctataac gcatttagta aaaaaataat tcatgaatgg tttattacct gattgaacat
      541 gaataaaagt aatattttta ttttgatggc ctatttctag gttatgaatt atccagatca
      601 aatttgtgta ttacattcag taataaggtg cttatcttag gatcaactta tgatactagc
      661 tgaccaagga tgtaccagtt atagaaaatc agttaaagta aaaatgataa aaatcttatg
      721 tcaacactga tttaggctga cctgtgggtt aggagaaaga caggtcggta gcttttaata
      781 caaaaaatgg cttgtatgta tctagatagt taactgaaat tagaaatgtt cttattttta
      841 acggagtaag aaaaataggc tgctttaggt ctgaatactt tttcaatata atgaagatat
      901 caaaggcgac aggagtgttt ttatgaataa gaatcctcat aatctaaagt tggtagcagt
      961 agatatggat ggaacatttg ctcgtactga ttacacatac gatatcccaa ggttcaaggc
     1021 tgttttatcg cgaatgaaag ctgttgattg tcaatttgtt gttgcaagtg gaaatcaata
     1081 ctaccaattg agatcgttat ttccaaatta ttatgatgaa ttaagttttg ttgctgaaaa
     1141 tggtgctttt gttaaagata aaacagaggt actattttca gccgatatgc ctagaaaagc
     1201 gatcgaggtg gtccttgatt tatgtaaaga ctatcctgaa gtaaaaaatg tgatgtgtgg
     1261 tctgaatagt gcctactgtg aaagaggaaa ggtgagtcaa gcattttttg atctaaccaa
     1321 tatctattac catagattag aatgggtagc tgattttgca gaagttcaag ataaaatttt
     1381 aaaatttgct ccgactatgc ctgttgaaaa aaccgaattt tacgttgaat tgttccgtga
     1441 aaaattagcc gggctattag tacctaccag tagtggtcat ggttctgtag acttgattct
     1501 ttctgagtgc cataaaactt tcggcttgaa aatgttagtt gagcgcttgg ggattaatcc
     1561 tgaccagtgc gttgcttttg gagatggggg caatgacatt gagatgttgg agtattgtgg
     1621 tctgagttat gaaatggata atgcaacgga agctgttaag caagttgcta agcatcaatg
     1681 tccttcaaat gacgaagacg gtgttttagt gacgctcgat cgattgttcc ctaacacgta
     1741 aacactaggt aattcatcaa aaaacctcga tataagaaat attcgaggtt ttaaaactat
     1801 atttagaaca gatttacgaa gcgtctgatg agtcaaaata gacttggaca ttgctatcta
     1861 aaataccact ttcattcttt aaacgaattg tttgatttga tggagttacc tctaatacta
     1921 aatgcccatt aataaaggta gtatcgtatt cagatgtttt aactgttgct tcttcgcaag
     1981 gtattgttaa atcatatgct ggaatataat gttgtgtgga aactaataaa aaatgatcac
     2041 caatcataca gcatccctct gctgaagaaa attgattaga gccatcattt aaagttaaca
     2101 agaatccatc ctttaaacga ggtacttgct taaatacttc tggttgtact tctaaaaata
     2161 aattatcgct cattttcacg accaccatcg acttccacag ttgaaattga cattttattt
     2221 aacacaccta ttccccctta actataactc aattctatat aaaaaatatt ctattctcaa
     2281 ataattgaac ttttcaaatc gccaatttac ttcggcttta tcagagctca ttcatatcaa
     2341 gagcgattaa cgctgtaaaa attaaaccga agatctgata aattttctct attttctaca
     2401 atatttaaaa cgcttcctta gtctattata tatctatttc tcttaggagt atgtttagat
     2461 gtttgaataa tagttgccca actttaaagg ttattcttat aatattttac ttcattttcc
     2521 aatagaaaat tcaaaggatt actggttttt ctaaaatagt ttagtttaaa tacataagta
     2581 tcctccgaca ctgtattagt tcataatcat ctaaatacaa aattttcaaa taaaaatatt
     2641 tcaagctcta tcgttgacga atgtaaacga tacttataaa gtaatagaaa ttgattacat
     2701 ttgtaaactt aaaatggtta atttatctca cataaaacta agaataaaat aatgattaaa
     2761 agtggttagt ttttatacga tttagcctat cggtaaaaat gaattagaga gtgtaaaata
     2821 ttttgtgtaa atgaaaaaat ccatacaaaa aaggaagtc
//
LOCUS       DAYYJC010000172         2725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000172 DAYYJC010000000
VERSION     DAYYJC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.179"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            399..>2725
                     /gene="esp"
                     /locus_tag="ACHA9N_002757"
     CDS             399..>2725
                     /gene="esp"
                     /locus_tag="ACHA9N_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9086162.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          984 a          421 c          539 g          781 t
ORIGIN      
        1 ttccgaagaa ggaatttgaa gtcacctttt tacttatgtc acagtctcat tcagtaatgc
       61 gggataaggc tccttattcc gttatttgaa tactctgaag tttaatctaa gaaagggtaa
      121 tattttgaaa acatgcttca tcgcatagaa atgaacttaa tttcagaaag aatacataca
      181 tatcttttta tatgttttca gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac
      241 atgaagaaat gtactttttt tgttgtaatg ttcatgaaat tctctaagta aattagttaa
      301 cataaggtta gtaagattcc tattctgtga ataatacgta tcagatttat cttttattct
      361 tactaggaaa gtaattatta agggagttga tttgaataat ggttagcaag aataataaga
      421 gagtatttct agaaaaaaca aagaaacgag tattgaagta cagtatcaaa aaattaagtg
      481 ttggggttgc atctgttttg attggtgttg gtcttgtctt gggaacaact gaactagtta
      541 aagcacaaga tgaaatttct ccaagcagcc cacttgaaac agcgataagc tccgtacaag
      601 taggtgacaa agtagcttcg gggaatactt ttcaggagaa tcctggttat acgaaaaatt
      661 ataacttttc ggatttacaa tttagtcctc aagaactaac tggagacaca ctaaaaggca
      721 atacaattgg atttgaagtt tacggaaaac ataatattgc agcatcaact aaaaactggg
      781 aaatccgtct tcaattagat gaacgattag ctaaatatgt agaaaaaatt caggtcgatc
      841 caaagaaagg aattggatct tctagacgga cctttgtaag aattaatgat tcgctgggta
      901 gacctacaaa tatttggaag gtcaattata ttcgtgcaag tgatggatta tttgctgggg
      961 cagaaacaac cgatacgcaa accgctccta atggggtaat tacgtttgaa aagagcctag
     1021 atgaaatttt taaagagata ggcatagaca atctcaagac cgatcgttta atgtatcgaa
     1081 tctacttggt aagtcatcaa gacgatgata aaattgtacc gggtattgat agtacgggct
     1141 actttttaac agattcagat gatttctata atagcttaga tgtatctgaa aataatcctg
     1201 accagtttaa acatggatca gtaagtgcaa aatatgagaa acctaatact caaacaaaag
     1261 atggttcagg atccacaggt gctaatggag ctataatctt agatcataag ttaacgaaaa
     1321 attataattt ctcatattca tcttccgcaa agggcacgcc ttggtatgct aattacaaaa
     1381 ttgatgaacg attagttcca tatgtagcgg gtatacagat gcacatggtt caggctgaca
     1441 aagtaacata tgatgtttcg tttgaatccg gaacaaaagt agctgattta gcaatcgagc
     1501 gacgggaagg tcatgaaaat tatggcatag gttcaataac taataatgat ttaactaaac
     1561 ttatcgattt tgctaatgca agtccacgtc cagtcgttat tcgatatgtt ttacaattga
     1621 ctaagccatt agatgaaatc ttagaagata tgaaagcaac agcacaagtt gaagaaaata
     1681 aaccatttgg tgaagatttc atctttgatt cttggttgtc ggatacgaat aaaaaattaa
     1741 tccagaacac ttatggaaca ggttattatt atttgcaaga tattgatggt gatggaaacc
     1801 ctgacgataa agaagagagc ggagacacga atccatatat cgggaaacct gaattagaag
     1861 aagtatatga tgttgacaca acagttaagg ggaaagtatt catccacgag ttagcgggaa
     1921 caggtcacaa agcccaactt gttgataaag aaggtactgt attagcagaa aaaactatcg
     1981 ctccaaatga aaaagatggg gctccaattt cagatactgt agaatttgaa tttacgggtg
     2041 tagattcaag taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt
     2101 ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg gttgataaac
     2161 aaaccgtggt agttggattt aaaccagatg ctaaagaatc aattcggaat aataaaaact
     2221 tacctgaaga tgcagagtat tcatggaaaa cagagcctga tacttctaac gttactgata
     2281 gtacgaaagg tattgtaact gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt
     2341 ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac atacgtacct ataacaacaa
     2401 ctccagaaac gacagttcaa agtggaaaac ctacatttga taaaccagat gttcctttag
     2461 ctaaagatgc cttttcaatt tcagatgtct ataataagta cttcggtaat gcaagtgttg
     2521 atgcaaacac tggtattgtt acattcactc cagctaaagg tgtaggagaa tcggagccga
     2581 ttactggaac aattcctatt aaaattgttt accaagatgg ttctgtaggc acgaccgatt
     2641 tagcagtaac tgtaagtaaa gatatttatg aaaatccagg agaaaacatt cctgcaggct
     2701 accacaaagt aaccttcacc gcagg
//
LOCUS       DAYYJC010000173         2718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000173 DAYYJC010000000
VERSION     DAYYJC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.180"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACHA9N_002758"
     CDS             complement(<1..153)
                     /locus_tag="ACHA9N_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9086163.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACHA9N_002759"
     CDS             complement(475..696)
                     /locus_tag="ACHA9N_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086164.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACHA9N_002760"
     CDS             complement(794..1039)
                     /locus_tag="ACHA9N_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086165.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="ACHA9N_002761"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="ACHA9N_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..2505)
                     /locus_tag="ACHA9N_002762"
     CDS             complement(2263..2505)
                     /locus_tag="ACHA9N_002762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086166.1"
                     /translation="MSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFK
                     RYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          923 a          475 c          378 g          942 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
     2701 actggaggat ttttcatg
//
LOCUS       DAYYJC010000174         2533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000174 DAYYJC010000000
VERSION     DAYYJC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.181"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..379)
                     /locus_tag="ACHA9N_002763"
     CDS             complement(<1..379)
                     /locus_tag="ACHA9N_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9086167.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            565..1968
                     /locus_tag="ACHA9N_002764"
     CDS             565..1968
                     /locus_tag="ACHA9N_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFM9086168.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1989..2273
                     /locus_tag="ACHA9N_002765"
     CDS             1989..2273
                     /locus_tag="ACHA9N_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFM9086169.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DAYYJC010000175         2516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000175 DAYYJC010000000
VERSION     DAYYJC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.182"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACHA9N_002766"
     CDS             complement(86..400)
                     /locus_tag="ACHA9N_002766"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086170.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACHA9N_002767"
     CDS             complement(451..705)
                     /locus_tag="ACHA9N_002767"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086171.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHA9N_002768"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACHA9N_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9086172.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACHA9N_002769"
     CDS             complement(1145..2098)
                     /locus_tag="ACHA9N_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9086173.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          790 a          443 c          365 g          918 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tactta
//
LOCUS       DAYYJC010000176         2443 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000176 DAYYJC010000000
VERSION     DAYYJC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.183"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..2292
                     /locus_tag="ACHA9N_002770"
     CDS             139..2292
                     /locus_tag="ACHA9N_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9086174.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          834 a          427 c          542 g          640 t
ORIGIN      
        1 agtgattgct gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag aatttcttgc
       61 tccgcccatt ttttcttttt tattaagaag tttttgggta caatgaagca tgaatgtaaa
      121 taggcggaag gataggaaat gatgaaagat tttatgaaaa aaatattaaa tacttcttgg
      181 ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat
      241 gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt tgcgttgatt
      301 gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct
      361 acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga ctccacagga
      421 aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg aacacaagtt
      481 acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga tgttccggta
      541 gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc caaacatttg
      601 aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat
      661 agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga gcaattaaaa
      721 attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt ctatatcaaa
      781 aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc tgaacttcca
      841 ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc actgaaaagt
      901 attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc agagaaatac
      961 ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga aaaacaatat
     1021 gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa tgaaggaaat
     1081 gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat gttatctatg
     1141 aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca aacattgatc
     1201 aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa tcctcagaca
     1261 ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga aatcaccgag
     1321 aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa ggctggtact
     1381 ttgacagcag gctgggcaag taacgcgatc agcggaaatc aagttttgat tgatgagccg
     1441 attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg acaagttgct
     1501 cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa agtggcgtta
     1561 aagatgttag gactagacta tacacctgga atgggactac ctagtttaga tgaagaagct
     1621 aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac aacgactggg
     1681 atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat gaagaagttc
     1741 aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt ggcatttgga
     1801 cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt cgctaatggc
     1861 gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga aaatggaaac
     1921 ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa tatttctgca
     1981 gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac cgatccttac
     2041 acaacagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac agggactgca
     2101 gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat cgtggcatac
     2161 ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt attagacgaa
     2221 caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta tgatatgttc
     2281 atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt
     2341 ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag aaactggctt
     2401 ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaaa ttg
//
LOCUS       DAYYJC010000177         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000177 DAYYJC010000000
VERSION     DAYYJC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.184"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHA9N_002771"
     CDS             5..1150
                     /locus_tag="ACHA9N_002771"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9086175.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHA9N_002772"
     CDS             1321..1779
                     /locus_tag="ACHA9N_002772"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9086176.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ACHA9N_002773"
     CDS             1938..>2407
                     /locus_tag="ACHA9N_002773"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9086177.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAYYJC010000178         2390 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000178 DAYYJC010000000
VERSION     DAYYJC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.185"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACHA9N_002774"
     CDS             197..2059
                     /locus_tag="ACHA9N_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9086178.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACHA9N_002775"
     CDS             2059..>2390
                     /locus_tag="ACHA9N_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9086179.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAYYJC010000179         2319 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000179 DAYYJC010000000
VERSION     DAYYJC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.186"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..1767
                     /locus_tag="ACHA9N_002776"
     CDS             154..1767
                     /locus_tag="ACHA9N_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9086180.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          730 a          410 c          448 g          731 t
ORIGIN      
        1 atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct
       61 gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca
      121 tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta
      181 gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt
      241 atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga
      301 ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa
      361 aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga
      421 ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc
      481 atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac
      541 ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa
      601 aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat
      661 ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct
      721 tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt
      781 catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat
      841 ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa
      901 gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag
      961 ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta
     1021 gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg
     1081 ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc
     1141 gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca
     1201 tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat
     1261 ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta
     1321 ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca
     1381 atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg
     1441 tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg
     1501 aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc
     1561 tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat
     1621 attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac
     1681 attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt
     1741 atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg
     1801 agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta
     1861 tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta
     1921 tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa
     1981 aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac
     2041 gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct
     2101 ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg
     2161 actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa
     2221 tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg
     2281 tattaaataa tttaattttt ttgttcctat ttatttaac
//
LOCUS       DAYYJC010000180         2312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000180 DAYYJC010000000
VERSION     DAYYJC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.187"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..677
                     /locus_tag="ACHA9N_002777"
     CDS             <1..677
                     /locus_tag="ACHA9N_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9086181.1"
                     /translation="INKINSITGQSLNFSYNKRKVLSNISFSTEMNKLFMISGENGTG
                     KTTLMKLFTKQLPVSNNQLFVNGIDINMIDNESLRRLISIATQIPYIINSSVKENILM
                     GTKNYDKKKFSKLIELLGISKFMTKLGITLDDNIYDEKILLSGGEKQKISLARALIKS
                     ASVIILDEPFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNK
                     EYDNNE"
     gene            670..1698
                     /locus_tag="ACHA9N_002778"
     CDS             670..1698
                     /locus_tag="ACHA9N_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFM9086182.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            1754..1972
                     /locus_tag="ACHA9N_002779"
     CDS             1754..1972
                     /locus_tag="ACHA9N_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086183.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            2043..>2312
                     /locus_tag="ACHA9N_002780"
     CDS             2043..>2312
                     /locus_tag="ACHA9N_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086184.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKH"
BASE COUNT          910 a          259 c          411 g          732 t
ORIGIN      
        1 aaataaataa aataaattct attactggtc aatcattaaa tttttcatat aacaaaagaa
       61 aggtattaag caatataagt ttttcaactg aaatgaataa actattcatg atatcaggag
      121 aaaatggaac agggaaaaca acactgatga aattatttac taaacagttg ccggttagta
      181 ataatcaatt attcgtcaac gggatagata ttaatatgat tgataacgaa tcattaagaa
      241 ggttaatttc aatcgcaaca caaataccat atataattaa tagctccgtg aaggagaata
      301 ttttaatggg gactaaaaac tatgataaaa aaaaattcag taaattaata gaactattgg
      361 gaataagtaa atttatgacg aaattaggta ttactttgga cgacaatatt tatgatgaaa
      421 aaatattatt atctggagga gaaaaacaaa aaataagttt agctagggcg cttattaaat
      481 cagcttcagt tattatatta gatgaaccat ttagaaattt agataatgaa actcgcgata
      541 atttgaggga ttatatacta aatacaaaaa aagaagtttg ttatataatt ataactcatg
      601 atcacattct agataatttt ttggattcaa atgatgaaat attatattta aataaggagt
      661 atgataataa tgaatagcaa aaaacttcag atgggtgaaa ttgttagagg tcctagtcaa
      721 atatctattg atgttacata taaatgtaat tttagatgta aacattgtta caatgcgagt
      781 ggtggagaca attgttttat ggataatgag ttgactgatg atgaactgtt agaacttaca
      841 aaagatatat gtaatttaaa gccatataat atttgtttct gtggaggaga aactttactg
      901 agaaaaaaaa tagttttatc ttgtgccact ttattaaaag aggcaggtat tcctaatatt
      961 gctattgtta ctaatggatc attagtaact caggatgttg caaaagaatt attcaaagca
     1021 ggtattaatc gatttcaagt aagtttagat ggaaaaaatg ctagtagcca tgaagcactt
     1081 agattatatc caaacagttt tgaaaaggca attaatgcta tcaaattatt tagagaattg
     1141 tccgaagaag tggatgttgc tttttgtcct actagtttta attacaatca attacgagaa
     1201 gtatatcaac tagtaaatac tcttaaagtg aagtctttaa gaattcaacc atttatgctt
     1261 agtggtaggg gaatgcaaaa tagggaaata gtgccaactg tttctcaata tagaaaacta
     1321 gtttctgata ttaactatat taaaagaaat gaagattata ccaagataga ttggggagat
     1381 ccaattgatc atatcatccg acacagaact ttattgaaag atttttcgaa ttttgtttct
     1441 attaggtcag acggaggatt aatggcatca ccttacttgc caattcaagt tggaaatatt
     1501 aaatgtcaca gtctttctaa atattggcga gcaggacttt ataaagtttt ttctttagat
     1561 ttagttcaag atatagctac tcaagtttct tcaatacaag atatgagtaa agaattaaaa
     1621 aaaggacata ttaaatggct taatgaagat atagaaattg atattctcga tgaatacaaa
     1681 catgatattt taatttaggt ttatcattgg aaagtttaga taaacaaagt acttttagaa
     1741 aggagggaaa aaaatgattt ctgaacctca aatgaataat atgtatgaaa gcccaactat
     1801 aatgggagct ggtgaagctg cgccaagagc ttgttcagca attatggtgt gtgtgttatt
     1861 agcatctgca ggagtaggta tccaagcagg agcggtgtat acttatgctg tagttgctac
     1921 agtagctgta gctgcagccg cagtttggaa aacagtagga ttagttggtt aggaattaat
     1981 aggagactaa ctgtctccta ttgattccaa tgattaagct aaggaggaat tttaagaaaa
     2041 taatgaaata caataattta aaaccgaaat tacttaatga gaacatagaa tatataagag
     2101 aagaaaatta tggatattta accatagttc ccaaaaatgg aactgaattg aaagataacg
     2161 ttttcaatcc aacagtaaga tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa
     2221 aaatagcgaa tggtttaaaa aagaagtttc ctagtttaga atatgaaaaa ttctatcaag
     2281 atgttgtagg aactattaaa tttttcaaac ac
//
LOCUS       DAYYJC010000181         2240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000181 DAYYJC010000000
VERSION     DAYYJC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.188"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..496
                     /locus_tag="ACHA9N_002781"
     CDS             <1..496
                     /locus_tag="ACHA9N_002781"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9086185.1"
                     /translation="FISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSPYYAQGN
                     LIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVV
                     KKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRT
                     QKFL"
     gene            624..1307
                     /locus_tag="ACHA9N_002782"
     CDS             624..1307
                     /locus_tag="ACHA9N_002782"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9086186.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1616..1689
                     /locus_tag="ACHA9N_002783"
     tRNA            1616..1689
                     /locus_tag="ACHA9N_002783"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1650..1652,aa:Arg,seq:tct)
     gene            1848..2207
                     /locus_tag="ACHA9N_002784"
     CDS             1848..2207
                     /locus_tag="ACHA9N_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9086187.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          744 a          347 c          453 g          696 t
ORIGIN      
        1 ttttataagt tctataacgt tgattatatt tttaattctt attcttctcg tcgtcttggt
       61 tccccgaatt tttggtttga ctccttatat tattgaggac aaagaaatga gtccatatta
      121 tgcacaagga aatctgatat atgtacgacc gaaaaataca aatgagattt tagtcggaga
      181 tattattaca tattatgaaa attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt
      241 tgaaaacaac cacgaagatt tttatacaaa agctgataca aagacttata ttgagcctgg
      301 agtagtaaaa aagagaaata ttatcggtag tcccatcttt cagattccat atattggact
      361 agtcctttca aaaacggcat ttgcatggat tcaggggtta ttctttacga ttgctttttg
      421 cctaacagga atcacagcat ggaatacctt tgtagagttg aaaaagaagc ggagacgaac
      481 acagaaattt ttgtaagaat gttaaaaaca taaatgtatt ccagagatcc aacgactctt
      541 ctgaacatga aacacgagaa aacactcatg cagcttgtag atttagtcag ttgttcgtat
      601 aatgtgtgaa gaggaggagt agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag
      661 aagaactatt ggattttatg cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac
      721 tggaagaaga agctcatgta gcagaagtac cagttattcc ccatgaaaca gttgtatttt
      781 tgaaattttt acttggacag cttcagcctg aacgaatctt ggagatcgga gcagctatcg
      841 gtttttcatc aagtgtcatg gccacgacaa tcggagaaaa cgctcatgtt acgacaattg
      901 atcgtttcga tgtcatgatc gagaaagcta aaaaaaacta tgaacgtcta ggactgacag
      961 ataaagtgac tttgcttgaa ggtcaagcag cagatatctt gccggagttg tctggtccgt
     1021 atgactttat ttttatggat agtgcaaaat caaaatatat tgaattttta cctgaatgct
     1081 tacgtttgtt aagaaaaggt ggcgttttga tggtagatga catcttccaa ggaggaacga
     1141 tcttgctccc agatgaagag attccccgtg gcaaacgagc tatccatcgt aaattaaatg
     1201 aatttcttcg tgttgtgatg gatcatcccg atttgacatc gacaatcttg ccattaggtg
     1261 atggtgtgat cttaatgaca aaggaatcag aaacaatcga gttgtgatac atgattatgg
     1321 cagcctgtag ataatcgatt gaatcgaggg ctcaattcga acttgatgta aaagtttcta
     1381 tatttgtaag tcaattaaag gatacaggaa atctaaataa acaggagtag catgaagtca
     1441 atgtagctcc tgttttcaat tgataacctt agaacagctg aaggagaatg tctcgtttta
     1501 aaagattgat ttagtagttg ggccaaaata attcattatt tgctaattaa ataactattt
     1561 cgtattgaca gaagcaattt aattatctat aattattgat gttccgctga ttcttgtcat
     1621 agtagctcag ctggatagag catccgcctt ctaagcggac ggtcgggggt tcgaatccct
     1681 cctgtgacgc taaaaaaccg ttagaaactg aggtttctaa cggtttttct ttgttttttg
     1741 tgccaatatt gtttgttttc aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa
     1801 ttataaggag caaaaaaata aaactcatca gaaaagagga agaagatatg acgatactat
     1861 ctagaatttt agtgactcta gttgcaattg aattttttta tatcatgtat atcgaaacgg
     1921 tgagaactga ttctgacacg acaagtaggg tttttaaaat gagtaaagaa gagttaagca
     1981 gaaaatctgt tcaaacattg tttaagaacc aaggagtgta caatggacta ttaggagtgg
     2041 gactacttta tggagcttat ctttcttccg catctaaaga aatcacaagt atgctattaa
     2101 tatctatttt ttttgttgca ttatatggca gtttgactag cgatcgttgg attttactca
     2161 aacaaggtgg gctagctctg ttggcattgc tttctatttt cttataaata tagaaaaaat
     2221 aagaatttat ctaacaggaa
//
LOCUS       DAYYJC010000182         2084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000182 DAYYJC010000000
VERSION     DAYYJC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.189"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            183..1676
                     /locus_tag="ACHA9N_002785"
     CDS             183..1676
                     /locus_tag="ACHA9N_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9086188.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1811..>2084
                     /locus_tag="ACHA9N_002786"
     CDS             1811..>2084
                     /locus_tag="ACHA9N_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9086189.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT          697 a          331 c          436 g          620 t
ORIGIN      
        1 gatcggatac cttttccgat tgcctttatt ctaccaaaaa aaggcgagca actcaacaga
       61 agaacgttta tttagtgtac ttaaattaaa tattcttaaa cactgaagag agaacacata
      121 ctatggctga gattaaagct aaactgtgtt ctctttttgt ttgagaaagg acgttacaat
      181 cgatgttgag cgaaatttat tcagctattc ttaaaaacgg cttacgtaca tacaaatatc
      241 gaaacagcac catgaaacct gcaggtcata ttgattccag taggagtggt gctatctttg
      301 cgtaccgtag caagcaatta atgaatgttg ggcgtggtat ggtcattact tcggaagaag
      361 ctattttaga gaacgaaaag aaactaacgc attggacacc taatacctat cgctttggta
      421 cttatgcgga tgattatcga acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa
      481 tcaacacatt tgtagttgat atcgattcaa aagagaacca tcaaggcgaa attattttag
      541 cctgtttaga tcaagtgggc tacatgccaa cgctaatttt agaatcagat catggttatc
      601 aagtttattt tgtactaaag acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga
      661 tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg
      721 gagtggatct aggatgtaac cacttcggaa ttgctcgatt cccacaagaa aataatattg
      781 tttttaaaga aatgaactat cagtattctt ttgctgactg gattcaatgg tcgatgaagc
      841 ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc tttcctgaga
      901 aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat gttacttcat aaagcaaaca
      961 tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat tttcacgctt tctttagctt
     1021 attactcttc aggttatgca ttggatactt gcgaatacaa tatgttcaca ttcaacgaac
     1081 ggttacagga accgctatct gaagcagaat tagtaaagat tgtacgtagt gcttattctg
     1141 gtaattatca agcagcaagc agagaattca ttattgaatt gtgccagcaa tgggtttcac
     1201 caaccttaaa aaataatgat ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc
     1261 cacgagatca aagaaaatat agtcataaaa atgaatggaa agaagatcta ttggcttatc
     1321 taagcgaaaa aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa gaattggctt
     1381 cacaactaac gatgcccaag cgaacacttg ataagatctt aaatgagtta cgagaggaga
     1441 ataaagtctt ctacaaggta acacgaggaa gaaatggcgg aatgatcatt gcttcagtca
     1501 aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa gagactagat tattcaacaa
     1561 ggatcacaga ggtatttgga ctcaacgaga gcttagtaga agagacattt agacaattat
     1621 caaccccctt gaaccctctt aaagaagtga atctttttga agtagataca ggttgaaaaa
     1681 taaaaggtgc aaattgcctt atacaatata tctatgatac ttctgtaggt gttgctccct
     1741 ttgcctagct acgtgtaggt ggtatactta taactgcgta tgcagtctta tgtatcatgg
     1801 aggaagttgt atgtcgttaa aaaattatgt ctattcacat cctgttaatg tgtttatcat
     1861 tggtcgtttg ggattatctg tggaagtgtt ctgtgagctt tatggtttta agcaggggac
     1921 tttgtcttct tgggttacca gggaaaaaac agttgcttct ttgccgattg agtttcttca
     1981 tgggttgtcc ttagcttctg gggagactat ggatcaggta tatgattgtt tgtgtgtgtt
     2041 agagcaggag tatattgagt ataaaatagc taacgagtta agaa
//
LOCUS       DAYYJC010000183         2064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000183 DAYYJC010000000
VERSION     DAYYJC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.190"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACHA9N_002787"
     CDS             <1..1008
                     /locus_tag="ACHA9N_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086190.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACHA9N_002788"
     CDS             1025..1174
                     /locus_tag="ACHA9N_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086191.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACHA9N_002789"
     CDS             1171..1617
                     /locus_tag="ACHA9N_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086192.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACHA9N_002790"
     CDS             1619..1756
                     /locus_tag="ACHA9N_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9086193.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2064
                     /locus_tag="ACHA9N_002791"
     CDS             1794..>2064
                     /locus_tag="ACHA9N_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086194.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          528 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actt
//
LOCUS       DAYYJC010000184         2061 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000184 DAYYJC010000000
VERSION     DAYYJC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.191"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            675..1007
                     /gene="traE"
                     /locus_tag="ACHA9N_002792"
     CDS             675..1007
                     /gene="traE"
                     /locus_tag="ACHA9N_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9086195.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1626..1967
                     /locus_tag="ACHA9N_002793"
     CDS             1626..1967
                     /locus_tag="ACHA9N_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9086196.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          718 a          275 c          433 g          635 t
ORIGIN      
        1 agccttttac gtcataagtg ttaaaagaaa taattaattt caaatataga aaagggaacg
       61 tatgtttgca tactcctttt ttttgagtta taataatacg cgaagagaga taatttcatt
      121 atttaaacaa gctaaaaatc ttgatattac ttcttttcag gcaatacgaa tattattcgc
      181 ttacattttt tgtataaatt taattgtctg ctatgcttgt gatgaattga ttttcgtttt
      241 gcacttttgg atgataaatt tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa
      301 ctcggaaggc tggtaacctt cgtcagcgtt catacacggc attaagattt ggcccttaat
      361 gcgttaacct ttcagtgtta cggtattacc cgcaatggat ttagtataac agatattcaa
      421 aagtgatacc actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc
      481 tatgcctaag cataatgtta taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct
      541 gaaattagag ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa
      601 atcaattctc tatttttgaa aaataacaag aaaaacttgt taaaaatatt taaagaaatt
      661 gggtggttta ttttatgtca tatacaggaa ttttgagtct caaagatatt tgtcactacg
      721 gtaaacggtg taccgctaca gagaaaatca cgaagaaact atcaactggc caaagtaaaa
      781 ccgttgtgca gtgtaagaaa tatataatcc agaaagacaa agtctcagag gaaatgattt
      841 attacattgg gaaacaaaag caaataattt taaaagatcc tatcccctta aaagagctat
      901 atccaacaat caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg
      961 gagtcttgcg ttgtaccgca aaagggatgg ggcgtttgat cggctgaatt gatcattctc
     1021 ataacttgta ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa
     1081 cttagagtca ctagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag
     1141 tgacggggta ggttttaacg attggtaacg taaaaaattg gtcaatcgag taaatgcgct
     1201 tgtttggata gtggtgaaat gaatttgtgt aaataataag cgcagagtaa acaagaggga
     1261 atttagaagt tagacaagtt agaatagcgt ctagattaat catgagcagc tactaaggga
     1321 tctagacttt gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg
     1381 cacaagaaat tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgattatt
     1441 ggatcaatcg agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga
     1501 gtcgaaattt tttatgtatt tttaagtaaa cgaaaaacga taaaatatga acaagtcctg
     1561 aataaagtga aaaaattaat ataaagcata gagaaaatga aatttacgta atggaggaat
     1621 gacaaatgaa aaattatgat ccgaatatac gattgggcac acatactatt aaggtttctt
     1681 tccaacgttg ggattacaaa ggatttgtga cgtttagaag gggcggaaat tgtaaaggat
     1741 tagatgtttt agcattagac gaggatgatt tatacgatca aacattgact gataatccaa
     1801 ttggattcgg cttgctacct gaagatgatg aaggtaatga atggtttaaa atgacgctta
     1861 tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg agtgattata
     1921 ttgtcagctt tgaaatcatt gaatttgtag cggataaaga agaataaaaa ctggaggaat
     1981 aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg tagtattacc
     2041 ttaggcattg agtaaggaat t
//
LOCUS       DAYYJC010000185         2058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000185 DAYYJC010000000
VERSION     DAYYJC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.192"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..337)
                     /locus_tag="ACHA9N_002794"
     CDS             complement(<1..337)
                     /locus_tag="ACHA9N_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9086197.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="ACHA9N_002795"
     CDS             complement(404..559)
                     /locus_tag="ACHA9N_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFM9086198.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="ACHA9N_002796"
     CDS             1176..1397
                     /locus_tag="ACHA9N_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086199.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="ACHA9N_002797"
     CDS             1398..1655
                     /locus_tag="ACHA9N_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086200.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="ACHA9N_002798"
     CDS             complement(1791..>2058)
                     /locus_tag="ACHA9N_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005727 - extrachromosomal circular DNA
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rep family protein"
                     /protein_id="HFM9086201.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DAYYJC010000186         2055 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000186 DAYYJC010000000
VERSION     DAYYJC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.193"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACHA9N_002799"
     CDS             <1..365
                     /locus_tag="ACHA9N_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086202.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACHA9N_002800"
     CDS             complement(721..1293)
                     /locus_tag="ACHA9N_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9086203.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHA9N_002801"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACHA9N_002801"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9086204.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAYYJC010000187         2040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000187 DAYYJC010000000
VERSION     DAYYJC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.194"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACHA9N_002802"
     CDS             <1..1872
                     /locus_tag="ACHA9N_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9086205.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DAYYJC010000188         2002 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000188 DAYYJC010000000
VERSION     DAYYJC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.195"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACHA9N_002803"
     CDS             <1..378
                     /locus_tag="ACHA9N_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFM9086206.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACHA9N_002804"
     CDS             426..1826
                     /locus_tag="ACHA9N_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9086207.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAYYJC010000189         1992 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000189 DAYYJC010000000
VERSION     DAYYJC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.196"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="ACHA9N_002805"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="ACHA9N_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9086208.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="ACHA9N_002806"
     CDS             851..1039
                     /locus_tag="ACHA9N_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9086209.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="ACHA9N_002807"
     CDS             1052..1609
                     /locus_tag="ACHA9N_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9086210.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="ACHA9N_002808"
     CDS             1641..1883
                     /locus_tag="ACHA9N_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9086211.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          308 c          406 g          555 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 tctttattcc tt
//
LOCUS       DAYYJC010000190         1879 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000190 DAYYJC010000000
VERSION     DAYYJC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.197"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1620
                     /locus_tag="ACHA9N_002809"
     CDS             <1..1620
                     /locus_tag="ACHA9N_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9086212.1"
                     /translation="TVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVT
                     YPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTV
                     TDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKV
                     EIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVK
                     VPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVST
                     AGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIK
                     NKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQV
                     DPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTAT
                     DESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGE
                     SDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1754..>1879
                     /locus_tag="ACHA9N_002810"
     CDS             1754..>1879
                     /locus_tag="ACHA9N_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFM9086213.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLE"
BASE COUNT          741 a          294 c          402 g          442 t
ORIGIN      
        1 acagtagatc taacagataa tgtaaccaac ctaccaacat taccagaagg aacaacagta
       61 acagatgtca ctcctggtgg tacgattgat accaacacac caggtaatta cgaaggggtt
      121 atcgaagtaa cgtatccaga tggtacgaaa gatacagtga aagttccagt agaagtgaca
      181 gacaatcgtt ccgatgccga taaatatgaa ccaacagtag aaggcgaaaa agtagaagtt
      241 gggggcacag tagatctaac agataatgtt actaacttgc cagcgttacc agaaggaaca
      301 acagtaacag atgttactcc cggcggtaca attgatacca acacaccagg taattacgaa
      361 ggtgtcattg aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa
      421 gtgacagaca atcgttctga cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta
      481 gaaattggag gcacagtaga tttaacagat aatgttacta acttgccagc gttaccagaa
      541 ggaacaacag taacagatgt tactcccggc ggtacaattg ataccaacac accaggcaat
      601 tatgaaggtg tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca
      661 gtagaagtga cagacaatcg ttctaatgca gataaataca atcctgaagg acaaaaagtg
      721 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagattta
      781 ccaaaaggaa ctacttatgc ttggaaagag aaagtagatg ttagcacagc tggaaataaa
      841 aaaggtacta ttgtagtgac atatccagat ggatcatctg atgaagttga agtagacgtt
      901 acagtgacag atactcgttc cgatgcagat aaatataatc ctgaaggaca aaaagtgact
      961 acagacttaa ataaagagcc agatgcatct gagggaatta agaacaaaga agatctacca
     1021 aaaggaacta cttatacttg gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa
     1081 ggtactgtag tagtaacata tccagatgga tctaaagaag aagtagaagt tactatttct
     1141 gtagtagata aaaaagcacc gaataaacct caagttgatc ctattacaga gggtgaccaa
     1201 attgttactg gtaaaactga accaaatgca gaggtgacag ttacattacc agacggaagt
     1261 caacaccatg ggacagctga taaaaatggt aattttacag ttaaagttcc taaattagag
     1321 gcagggacaa aagtgatagt aactgcaact gatgaatctg gtaatactag tgaacctact
     1381 aatgtagttg tttcttccaa caaaaaagac agtggaaaaa acggtagtaa aggtagtaag
     1441 actgacaatc aaggtagcaa cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca
     1501 aaagtttttc ctaagacggg agagagtgac agtaatatct ttactattag cggtggttta
     1561 atactgttag gaacgttagg attattaggg tataaaaatc gaaaaaaaga gaatgaatag
     1621 aaaatcaatt tcttaagtct tctctgagaa gaggaagaat ctttttcttc ctctttttgc
     1681 ttttttatga tgggaaataa gtgttagtta aagatgaata tactttttat agaaaggaaa
     1741 agaatgtaat taagtggaaa aaatagtaat tattggttca aaccattcag ggctagccgc
     1801 agctcgattt ctaaacttaa gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc
     1861 agaaattagt tacttggaa
//
LOCUS       DAYYJC010000191         1877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000191 DAYYJC010000000
VERSION     DAYYJC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.198"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHA9N_002811"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHA9N_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHA9N_002812"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHA9N_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          656 a          265 c          248 g          708 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacg
//
LOCUS       DAYYJC010000192         1858 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000192 DAYYJC010000000
VERSION     DAYYJC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.199"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..531
                     /locus_tag="ACHA9N_002813"
     CDS             <1..531
                     /locus_tag="ACHA9N_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086214.1"
                     /translation="IQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGG
                     EPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDF
                     NDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMK
                     NILKNVKIAESLDDIL"
     gene            528..>1858
                     /locus_tag="ACHA9N_002814"
     CDS             528..>1858
                     /locus_tag="ACHA9N_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9086215.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMII"
BASE COUNT          651 a          220 c          331 g          656 t
ORIGIN      
        1 atacaaattc cattagttac agctgggtca agaaccgcat tgcaactttc ggatattttt
       61 aaccctatga tagatattta taaagaaaaa aaatatatat tttttacgtc tgtttatgat
      121 tttgagggcg gagaacctgt cggagaatat gaattagtat gcaaaatagc agacttagta
      181 ggaaaagata atctaattgt taagacgcat cctagagatg taagaacaat ttataaagat
      241 aatggattta tcgaggataa aaattcatca ataccttggg aggcgattca attgtcaggc
      301 gattttaacg ataaaatatt tatgacaatt aattcaggaa gtgttatttc aggtagtaca
      361 atgtctgcaa aacctgtacg cacgtattat atgtataaat tgtgtaacat agaaggaaat
      421 ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc ttaagcaaaa aacgatgaaa
      481 aacattttaa agaatgtgaa aatagcagaa agcttagatg atatactatg agcaaaacta
      541 aatatttaat taaaaatatg ggaatattaa caataagtaa tttttcttca aaaattttag
      601 tttttttgtt agtcccttta tatacaagtg tattatccac aacagaatat ggcacatatg
      661 atttgacaat ttcaacagcc acattattgt ttccaatttt aaccttaaac attgtagatg
      721 ctgtgatgcg ctttttaatg gataaagata aaaataaaaa aaatatagca tctatagcga
      781 ttaaagttat atttttaagc gtgatagtct ttggatccgt aatgtatttg ttaaatatta
      841 caaaattatg gcaagatgtt tatggattag aaaaatatat ttttttgtat tatatatcct
      901 atgtatggaa tcaatttcta atacaatttg ctaaagggtt agaaaaagtt aaagctatgg
      961 gcatagcagg tattatcagc acaattgtaa caataattgc caatattttc ttcttgctta
     1021 ttctgaaatg gggattaatt ggttttttca ttgcaaatat tttatcacag attatttcag
     1081 ctacttatct gtcagtatta gtgagaatat gggaatatat cgattttgag gctaaagatc
     1141 ctcagctaaa gagagaaatg ctaaattact cgattccatt aattgcaact gttgttggat
     1201 ggtggattaa tagtacagct gataggtatg tggtttcctt tatagtaggt gtctcggcga
     1261 atggcttatt gtctgtggca tataaaatac ctcaaataat taatactttg cagggaatat
     1321 ttatacaggc ctggcaaatt tctgcaatta aagaatatgg agataatgag actgcaaaat
     1381 tttatggaaa tacatttgtt atgattaatt tgttaatgtg cataacctgt tcaagtctta
     1441 tcatattaac tagaccgttt tcacatattc tttatgcgaa agacttttat gaagcgtgga
     1501 aatatgttcc atttttatta atagctaatg ttttaaattg tgcatcaggt ctgttaggac
     1561 ctatcttatc agcaaaaaaa gattctaaat ccatgatgtg gtctgcaatc ataggggcat
     1621 tagttaatgt gattatgagt gtagtccttg tttataagat aggggttcaa ggtgccacaa
     1681 tagccactgt tatatgttca tatgtaatat atgttgttcg taaacgagct gtgggaaaag
     1741 atatttttat aaagcattat catattgttg tggaatcttg gggattattg tttgttcagg
     1801 caatcataga aatatatttt aaaaattatg taatagaaat tataattatg attatttt
//
LOCUS       DAYYJC010000193         1830 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000193 DAYYJC010000000
VERSION     DAYYJC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.200"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="ACHA9N_002815"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="ACHA9N_002815"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9086216.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="ACHA9N_002816"
     CDS             1281..1727
                     /locus_tag="ACHA9N_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9086217.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          625 a          275 c          404 g          526 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag
//
LOCUS       DAYYJC010000194         1786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000194 DAYYJC010000000
VERSION     DAYYJC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.201"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACHA9N_002817"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACHA9N_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9086218.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          499 a          307 c          352 g          628 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagt
//
LOCUS       DAYYJC010000195         1712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000195 DAYYJC010000000
VERSION     DAYYJC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.202"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA9N_002818"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA9N_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM9086219.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHA9N_002819"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHA9N_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9086220.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          504 a          296 c          273 g          639 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg tt
//
LOCUS       DAYYJC010000196         1678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000196 DAYYJC010000000
VERSION     DAYYJC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.203"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHA9N_002820"
     CDS             316..570
                     /locus_tag="ACHA9N_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9086221.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHA9N_002821"
     CDS             571..888
                     /locus_tag="ACHA9N_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9086222.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1678)
                     /locus_tag="ACHA9N_002822"
     CDS             complement(1522..>1678)
                     /locus_tag="ACHA9N_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9086223.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          590 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaa
//
LOCUS       DAYYJC010000197         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000197 DAYYJC010000000
VERSION     DAYYJC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.204"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..811
                     /locus_tag="ACHA9N_002823"
     CDS             95..811
                     /locus_tag="ACHA9N_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9086224.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            830..>1664
                     /locus_tag="ACHA9N_002824"
     CDS             830..>1664
                     /locus_tag="ACHA9N_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFM9086225.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LP"
BASE COUNT          644 a          197 c          318 g          505 t
ORIGIN      
        1 tgtgttatag aggaagaaac attatttcaa tgattaagag tcgagagcac aaacggtaga
       61 cgatgttatt tttataaaaa gatataattt attaatggtc gataattatg aaaggaaaaa
      121 tattcttaaa aaaaagagta taggatgtcg tatgaaaata gtcgctatag taccaatgaa
      181 gttaaataac aaaagattgc cacaaaaaaa cacgaaagct tttaccaatg gaaaaccatt
      241 gtgttattat atactttcaa cacttttaaa agtaaaacag attgataaag tgtatgtcta
      301 ttgtagtaac ccagatatta aagaatttat accagaaggt gtaacatttt taaagagatc
      361 tagttcctta gatcaggata caacgagtat tacacaggta ttagccggtt tcacaaaaga
      421 agtagaggct gatgtatatg taatgacaca cacaacagca ccatttatta gcgaaaaatc
      481 tattgaaaaa ggactagatg ccgtgctgac aggagaatat gattctgcat ttgctgcaaa
      541 aaaattgcaa gatttcttat ggaaagacgg tgaaccattt aattatgaat tggacaatat
      601 tcctagaaca caggatttgc cgcctttata cgaagaaact agtggatttt atatttataa
      661 tcgcaatgtg atgaccgagt tgcatagaag aattgggaaa aaaccttgca tagttgaagt
      721 aggtgagatt gagtcagttg atattgatga agccgaggat tttatgattg ctgatgcgat
      781 ttataatcat cttattttac aaggtagtta agacaggggg aaggttagga tgaacaatgt
      841 acagatttta gattgcacac ttagagatgg gggctattgt aacaattggg aatttggatt
      901 tgataataca aaaaaaatta tttctggact tatagaatca ggaattgaga ttatagaatg
      961 tgggtttctc acaaataaaa cagtatacaa taaaaatgtt acaaaatata cgaccttaga
     1021 agaattagca cagataattc ctgaaaaaag agaaggaaaa ttatttgtag caatgatgaa
     1081 ctatggcgaa tataatatag aggatcttcc agattatgat ggttcatcta ttgatggaat
     1141 taggattgcg tttcataaaa aaaattgcaa tgaagcgttg aaattatgtc gtgagataaa
     1201 gaaaaaaggc tacttagttt ttgttcaagc tatggtatct ctcaattata cagatgaaga
     1261 gtttttagca gtaataaaaa atgtgaatca gtttaaacca tacgcttttt atattgttga
     1321 tagttttgga atgatgaaag gaaaagattt aactcgtctt ttttacatga ttgagcataa
     1381 tctagaaaaa gatatacaga taggttttca ctcacataat aacatgcagc ttgcatattc
     1441 taatgcacaa aaacttataa ccgtacaaac aaatcgaagc ctgattattg attcttcggt
     1501 gtacggaatg ggaagaggag caggtaatct taacacagaa ctatttgtag aatatttgaa
     1561 tgataatgca ggaaaaaaat atcaattaaa accattatta tatataattg atgaaatact
     1621 aaatagtttc tatcagaaga gttattgggg atattcatta ccaa
//
LOCUS       DAYYJC010000198         1566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000198 DAYYJC010000000
VERSION     DAYYJC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.206"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1522
                     /locus_tag="ACHA9N_002825"
     CDS             56..1522
                     /locus_tag="ACHA9N_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9086226.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          291 c          330 g          511 t
ORIGIN      
        1 tttcggtaaa gatgtataat attttctgaa aatttaaaga attggggtat agattatgga
       61 aaaacagcaa gacaagggct tcttcgggca gccgaaggga ttgtcaactt tgttcttcac
      121 tgagatgtgg gaacgattta gttattacgg tatgcgtgca ttacttatct attatatgta
      181 cgatacagtt gccaacgggg gacttggatt accacgagca acagcattag ctatcatgtc
      241 gatctacgga tctttgatct acatgtctag tatcgtcggc ggatgggtat ctgaccgagt
      301 gttaggagca aaacgcactg tattcttcgg tggagttttc attatgcttg gccacatcgt
      361 cttggcttta ccatttggtg taagtacatt gtttgtttcg atggcattga ttattatcgg
      421 aaccgccttt ttgaaaccaa atgtttctgg catggtcgga catctttatt caaagactga
      481 cttgcgaaga gatgcaggat tttcgatttt ttacatgggt gtcaacttag gggcattatt
      541 agcaccacta atcgttggta caatcggtca agaatataac tatcatttag ggttctcttt
      601 agctgctatt gggatgttct ttggattatt acaatacatc atccaaggac gcaagacatt
      661 agacggagtg ggtacaacac cgccgaatcc tttgacaact gaagaacgta aaggattcat
      721 ccgtaacttg atcatcgcac ttgttgttat cgctgcagta ttcggcgggg cacaactaac
      781 tggtcatctg acaattgact tttttgtcaa tacaatcagt gtattgggga ttatcttacc
      841 gttgtattac tttatcaaaa tgctgacttc aaaagatgta actgctgaag aaaaaccaag
      901 agtatgggct tatatcccat tattccttgc agcaatcatt ttctggtcat tacaagaaca
      961 aggttcatct atcttagctt tatttgctag tgaacgtaca caatctacgc tatttggtgt
     1021 aacgatccaa gcaagctggt atcaatcatt gaatccaatt ttcatcttta ttctgacacc
     1081 agtattcgta acgatgtgga cgaaacttgg tgaacgtcaa ccatctacag tagtgaaatt
     1141 ctctcttgga cttttatttg caggattatc gtacttgcta ttgatgttgc cgggtatgct
     1201 ttatggtaca agtggaaaag taagtccatt gtggttgttg ggaagtttct tcatcgtggt
     1261 tattgctgaa ttgtgtcttt caccagttgg tttgtctgtg acaacaaaat tagcaccacg
     1321 agcatttgaa tcacaaacaa tcgctatttg gttcttagct gatgcttctt cacaagcaat
     1381 caatgcacaa attgcacgct tctacacacc gggaacagaa tctgcttact tcggtattat
     1441 cggtgcagta gcaatcgtcg gtggtatctt gctatttact gtgaaagaac caatcaaaaa
     1501 actaatgggc tctattcatt aatcaatgaa cataaaaaca ggaattgctt atttgcagca
     1561 gttcct
//
LOCUS       DAYYJC010000199         1553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000199 DAYYJC010000000
VERSION     DAYYJC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.207"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          586 a          177 c          315 g          475 t
ORIGIN      
        1 acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt taatttgaca
       61 attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat tggcgaacaa
      121 atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc agatattcgc
      181 gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta ttgctttggt
      241 acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg tttgagtgat
      301 gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta attttataaa
      361 gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata agcaaacgat
      421 gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg aaataatgca
      481 gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa aaatattata
      541 actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat tcataagcta
      601 tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc aggggcagaa
      661 cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta tgaaaatgga
      721 ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg aatttttcag
      781 aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct gtgaagaagc
      841 gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat tatctgattt
      901 tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt ggatattgga
      961 atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg cataagaata
     1021 ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga attttttgtt
     1081 ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat gctaagcgga
     1141 gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac ttatttcaaa
     1201 caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat cctcaaatcc
     1261 cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg atccgaaaaa
     1321 tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg aaattaccat
     1381 gatgagaaac acattaatta aagggaatta attaactaga atataatctg attcaatttg
     1441 aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta agttagaaaa
     1501 ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa caa
//
LOCUS       DAYYJC010000200         1451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000200 DAYYJC010000000
VERSION     DAYYJC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.209"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACHA9N_002826"
     CDS             105..1406
                     /locus_tag="ACHA9N_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9086227.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DAYYJC010000201         1451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000201 DAYYJC010000000
VERSION     DAYYJC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.210"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACHA9N_002827"
     CDS             complement(<1..625)
                     /locus_tag="ACHA9N_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9086228.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACHA9N_002828"
     CDS             complement(784..1050)
                     /locus_tag="ACHA9N_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9086229.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACHA9N_002829"
     CDS             complement(1040..1396)
                     /locus_tag="ACHA9N_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9086230.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DAYYJC010000202         1439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000202 DAYYJC010000000
VERSION     DAYYJC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.211"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1399
                     /locus_tag="ACHA9N_002830"
     CDS             164..1399
                     /locus_tag="ACHA9N_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086231.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
BASE COUNT          478 a          147 c          248 g          566 t
ORIGIN      
        1 tgactggaaa tagaaattaa atatattgta ttgttacatt aaagaaaact tgtatggaaa
       61 aatcagtaca ataccattat ttaatgttct tcacttaaag cgcaaaaaat atcagtttat
      121 taaactcgta tatagaacaa atatatgtag acggggcgaa acgatgagtg taaaaaaaat
      181 gctatttatg gtaagtacaa taattgtatt aacatcccga ttttggggaa ttaatactag
      241 tttatctatg cgatatttta ttatggcaat atgggattta tattttatgt taagctattt
      301 ttcttatcgt gagaaaacat ataaaaaata cgttatcaag aataactttt ggttgactat
      361 aatgccgttt gtgatatttt ctatctatac tctaataata tggggaattg gttcaaatgc
      421 agaatttgaa aattttataa agttatgttc cacgttgctt tatttaattc ttgcatatgg
      481 ttacgcatgt acagcatttt acttatttaa ggcagaagga attgatttta ttttttggtc
      541 aggtgttatt tcatatatga gtggttcagt tttttattta attgtatctt atggtttaaa
      601 tggtttaatt tcttatataa aaagtcttat aacaggtgaa acaaatactg caaattttgc
      661 tatggaggta catgatttaa cttttgcgat gggggttttt ttcctttatt acgtattttt
      721 tgaaaataaa aatacaaaaa aacataagat aaaaattatc gtatctttaa ttttaatttt
      781 catgggattg aagagaattg aactgttagc tttaagtggt gcaattctag tgtattatgt
      841 tttattaaag tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta tttgtgcaac
      901 tgttatttcg ttagtgttta tttacattat agatacagga attttggcat tgcttatgtc
      961 aaatttgggc attacagatg acggtgcgag gctttcattt tatctatatt catctaaatt
     1021 ttatgaactt ggtttaggat atgtaggaac aggatggaca tggttttcaa aatacttttt
     1081 taatctttat ttatcgcact ttaggttaaa tggtggaaga attgcagcaa gcctacacag
     1141 tgatatatta actatttata tagaagttgg atgtatagtt tttattatat ggatgttttg
     1201 gttatttttt gtgaaaccga taagactaac taaaaaattt ggattgaaaa caggggagtg
     1261 tgtattatta ttaactatat atatgtttat attatatttg acggataata cgctcactta
     1321 tccagacact caaatgattt ttctcttagt acctatgata gtttcgtggg ttgataatca
     1381 acaacgaata tcggaataga tagctaaagt atattttaat tatgaattta taattagtt
//
LOCUS       DAYYJC010000203         1417 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000203 DAYYJC010000000
VERSION     DAYYJC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.212"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACHA9N_002831"
     CDS             complement(150..1337)
                     /locus_tag="ACHA9N_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9086232.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAYYJC010000204         1410 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000204 DAYYJC010000000
VERSION     DAYYJC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.213"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..993
                     /locus_tag="ACHA9N_002832"
     CDS             <1..993
                     /locus_tag="ACHA9N_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9086233.1"
                     /translation="FIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTS
                     IILSLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANS
                     KFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNES
                     DKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFL
                     KKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSK
                     VNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            995..>1410
                     /locus_tag="ACHA9N_002833"
     CDS             995..>1410
                     /locus_tag="ACHA9N_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086234.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNV"
BASE COUNT          467 a          169 c          235 g          539 t
ORIGIN      
        1 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
       61 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
      121 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
      181 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
      241 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
      301 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
      361 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
      421 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
      481 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
      541 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
      601 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
      661 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
      721 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
      781 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
      841 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
      901 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
      961 atttcttact tgtatttaaa aaaggagaat taatatgaag acagctttca ttgcttggac
     1021 tcctttacat ttgattaata tattaaatgt ttatcaaaat ttttaccgag aatcaaaagg
     1081 agatttattt atttattctg aatttgaaaa cgctagaaga tatagtgagc gtataaaaga
     1141 attagggcta tttaatgatg ttgtttttgt gaatcaccgg aaaatcggtt cttcctttaa
     1201 acgtaaaatt aacttgttaa ctaaccataa tcaatttttc tcggatgatt tatccgttta
     1261 tgatgaaatt tttatacagg gggaaaatta tttcacaaaa atacttttct ccaatgttta
     1321 caaaaaaaat ccagatgtaa aactaaatta tatagaagat ggtttgggta cttatttgaa
     1381 gaagagtata ttttcaacaa gtaaaaacgt
//
LOCUS       DAYYJC010000205         1386 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000205 DAYYJC010000000
VERSION     DAYYJC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.214"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..468)
                     /locus_tag="ACHA9N_002834"
     CDS             complement(169..468)
                     /locus_tag="ACHA9N_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9086235.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            819..>1386
                     /locus_tag="ACHA9N_002835"
     CDS             819..>1386
                     /locus_tag="ACHA9N_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9086236.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKN"
BASE COUNT          467 a          220 c          195 g          504 t
ORIGIN      
        1 gtttatacta gctgtttttg tgttaattaa aggataaaat acaatataaa gaaacatctg
       61 tttctttatt tagaataaaa aaatacagac tctgttcaaa taaatgaacc tagcctgcat
      121 tatgagattg actatacttt ttaattcatt gagatgaata actcgttttt ataaatgccc
      181 aaaagtaatc ggtgccgaag ccattcctcg acctaattct ttttcaaatt ttcctattgt
      241 tgatatctcc atataaaacg gtgatactgg tttagacaat tctcccctga ttgctaacgg
      301 acgtaaacta cgttccaaat tatttatgac tttaggataa tactgacctt tttctaagcc
      361 tgtctttgcg tctatcaaaa gtatacgttc cttttctttg atctctggat ttaaaacgag
      421 atcatataat tttagtacca tgttttctat ttcttcactt ttagacatca cgatttcctc
      481 ccaaaaatat atttcttccc actacattct ataaacaaag tatacgctac atctagattt
      541 ttttaaatca ttactggatt gttaaaacct ttcaatacac tgttatggca acgattttta
      601 ttttaaaaac attctaaatt aaatcagtaa aaatttttat tctaagattt ttattagaat
      661 tacgcctaat ttgtgtctct cagggcaatt ctaagaacgt ttcaagagat agcgacacta
      721 tcgttatcga aaaaggcgaa aacctatctc attttctgct aatgtaagtt ggtttagctt
      781 ttgttaatat gactttatga aaatggaggt aattaaatat gatttttcct aaagttaatg
      841 aagcaagctt gaataaagga tccttttttc atatctatga aaacgaagat tttgaactgt
      901 ataatatcct ttcacaaaaa gaggtcatcg aaaaccaaat aatctgccat ccaattattt
      961 acaaatctat tttcaagtat gcattttttg cttccaatga ttttcaaaga atggagatta
     1021 ccgactgtat attcgaaaac tgtgattttt caggctgtat attgacaaaa agcatgttgc
     1081 atcgcgtgaa atttgtcaat tgtaaattaa caggtactaa attgatggaa agttatattg
     1141 ggaacacgtt attcgagaat tgtaaaatgg actatgtaaa tttatctggt tcaaacataa
     1201 aagaaagtac ctttgagcac tctgtcttat caagcgcaga ttttgtagac tgttccctca
     1261 ctaaaataat gttttctact aatgatttag aaaatgctag cttttttgat actgatttaa
     1321 aaaatatcga tttaagtagc aactcctttg aaatgattga agtacgctcg gaacatctaa
     1381 aaaatt
//
LOCUS       DAYYJC010000206         1363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000206 DAYYJC010000000
VERSION     DAYYJC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.216"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..>1363
                     /locus_tag="ACHA9N_002836"
     CDS             135..>1363
                     /locus_tag="ACHA9N_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9086237.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKK"
BASE COUNT          434 a          174 c          219 g          536 t
ORIGIN      
        1 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
       61 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      121 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      181 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      241 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      301 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      361 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      421 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
      481 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
      541 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
      601 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
      661 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
      721 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
      781 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
      841 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
      901 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
      961 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     1021 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     1081 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     1141 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     1201 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     1261 acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt
     1321 attttttact ttttattttt cattataata tcgctaaaaa aat
//
LOCUS       DAYYJC010000207         1363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000207 DAYYJC010000000
VERSION     DAYYJC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.217"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..>1363)
                     /locus_tag="ACHA9N_002837"
     CDS             complement(62..>1363)
                     /locus_tag="ACHA9N_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9086238.1"
                     /translation="KTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTEI
                     VDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFGV
                     YFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGNQ
                     PIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELDG
                     AFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNWI
                     FDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFLL
                     VGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSDC
                     LLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          440 a          245 c          200 g          478 t
ORIGIN      
        1 tctttgaaaa aaacataaat cagctaataa atatgtgaga caaagctgcc atccataaat
       61 actagctgat tttttcagtt aaatgtctat ttttttctct gatgatggta tttccaaaat
      121 attcccagtc atctggagtc ggtggatttt tccaaattat ctgttcttta ttaaattttt
      181 caagtaaaca atcagataaa taaatattcg tacgagaagt gattcgactc tcaatcacaa
      241 tattgtaatt tttaaatcct ttcacttgag aaataatgta atcggtataa gcagatcctt
      301 gcgaaaaatc aatacagaca tgaatcggtg cttcaaaata tgataccgga caaataccga
      361 ccaataaaaa gagataatca taaaataagc gaatcatgaa acgttcatct tttcccaaga
      421 aatagcgttt attctcttgt atgacctttt ggattgctgt actgaatatt ggatacgtct
      481 cgaagaaaaa ctgtagctgc tttttagacg taaagctagt catctcaaag tcgaatatcc
      541 aattttttcg attgacatgc atcataccca tacgaagttc ttttcgtata tatagtcttt
      601 cttcttcaga gatttcaatc gaaaatggta attgatcaat caattccact gacatttttt
      661 gactaatcat gtggatatca tgaaaatgag cttccgcaag tccaaatgcg ccatctaatt
      721 caaattcttc ttccgtgatc aaaaacaaca tgatataaga aatctcatca atgatctgct
      781 ctttagggat atgaagaaaa gaaatatgtg ataaatcctt gatgatcttc attccttctg
      841 agctcttaaa gtattcatct gaaaagaaac gttttccgat tggttgattc ccataggccc
      901 tcaacaaact aatagtggtt agcaaattga atttgattcg ttttgttgca gacagtttta
      961 aattgaacag atattcaaaa tattgcacga tactctttgc ttcattcatg acgctttctc
     1021 taaatggcat agaaaaacca ttaaagaccc cgaaatatac cccaaataat acgttccgta
     1081 tgtttatttc ttccccaact aatatgtttt tctttaactt tatttgatgc tgttcaagaa
     1141 aagagactaa ttctcttctt tttacatagg ctcttgatat gcttaaaaaa tgattctctg
     1201 caaatttttc gatcgttact tttgaatcca cgatctccgt taacagtcga gcaatttttg
     1261 atcgttgaaa gtagattctt cttaaatttt tgatatccgt caaattcaga cgttcgacat
     1321 ggatctctgc ttcttctgaa atttggatct ctggctttgt ttt
//
LOCUS       DAYYJC010000208         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000208 DAYYJC010000000
VERSION     DAYYJC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.218"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA9N_002838"
     rRNA            <1..1355
                     /locus_tag="ACHA9N_002838"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYJC010000209         1339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000209 DAYYJC010000000
VERSION     DAYYJC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.220"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACHA9N_002839"
     CDS             <1..1309
                     /locus_tag="ACHA9N_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM9086239.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          569 a          102 c          226 g          442 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttatggaatt aaaaatatta aacaggaatt
     1261 tatcgaaaat ggtagaaaag aaatttataa aaggacttat aaagattgat tatataatat
     1321 atgaaaagct attataaaa
//
LOCUS       DAYYJC010000210         1314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000210 DAYYJC010000000
VERSION     DAYYJC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.221"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..>1314
                     /locus_tag="ACHA9N_002840"
     CDS             14..>1314
                     /locus_tag="ACHA9N_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086240.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKN"
BASE COUNT          442 a          170 c          177 g          525 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
     1021 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
     1081 atttatttct ccacagttgg aaaaattttc ttatacaagg agaagaaatt ttatactatt
     1141 attaatatta gctatttcaa ttttaaatta ttcatcagta gttaacatgg tgaaagaaat
     1201 gaattttcaa ggaacttatt ctaatgaaca aatggaaaat aaacttgaag aatttgagaa
     1261 ttttgattat gtacctatag gctataaaaa agatcaattg cttttgaaaa acca
//
LOCUS       DAYYJC010000211         1294 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000211 DAYYJC010000000
VERSION     DAYYJC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.223"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACHA9N_002841"
     CDS             <1..1244
                     /locus_tag="ACHA9N_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9086241.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAYYJC010000212         1287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000212 DAYYJC010000000
VERSION     DAYYJC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.224"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACHA9N_002842"
     CDS             <1..848
                     /locus_tag="ACHA9N_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9086242.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DAYYJC010000213         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000213 DAYYJC010000000
VERSION     DAYYJC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.225"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHA9N_002843"
     CDS             200..742
                     /locus_tag="ACHA9N_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086243.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACHA9N_002844"
     CDS             898..>1246
                     /locus_tag="ACHA9N_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9086244.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAYYJC010000214         1240 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000214 DAYYJC010000000
VERSION     DAYYJC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.226"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1050
                     /locus_tag="ACHA9N_002845"
     CDS             43..1050
                     /locus_tag="ACHA9N_002845"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9086245.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1062..>1240
                     /locus_tag="ACHA9N_002846"
     CDS             1062..>1240
                     /locus_tag="ACHA9N_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFM9086246.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQK"
BASE COUNT          490 a          159 c          237 g          354 t
ORIGIN      
        1 aatcgaatta tagatgacta taattcatag gaggagatat aagtgtttaa aggaaaaaca
       61 atattagtaa ctggcggaac tggttccatt ggaagtgaaa ttgttcgtca gcttttaaaa
      121 tatgaaccaa aagctatcag aatttatagt agaggtgaag aaaaacaatt ttatatgcaa
      181 caagagctct caaatcacac taatttgaga tttttaattg gtgatatcag agactctcaa
      241 aggattgatt atgcatgtcg tgatgtggat attatttttc atgctgccgc aatgaaacac
      301 gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa caaatgtgat gggaactcaa
      361 aatgtaatag atgcagcgat caaaaataat gtaaaacatt ttgtaggtat cagtacagat
      421 aaagtagtga gtcctactaa caccatgggt gcgacaaagt tattgagtga aaaattaata
      481 atagctgcca atcagtataa aggaaaaaca gaaacaatat tttcttgtgt aaggtttgga
      541 aatgtcatgg ggtcaagtgg atctgttatt cctcttttta gtaaacaaat aaaaaacaat
      601 aaagatatta ctttgacaga tgttgatatg acacgattta tgatgtcaat accggatgct
      661 gcaagattag ttctgtctgc tgctatgaat tcaaaatttg gagaaacata tgtcttaaaa
      721 atgccggcaa tcaatatttt aactttagct caatcattaa taaaaatata taatgataca
      781 catgatgaaa aatatagagg tgaaataaaa gttgtaggta tcaggccagg agaaaaatta
      841 tatgaagagc taatgactac agacgaaatt gaacgctcat atgaaaatga taaaatgtat
      901 atcattccac cacactatca tgctactgac gaactgtaca aagattatga aaaaattaaa
      961 atcactgcat actcttcaga tgcaacagaa ttaatgagtg gacaggaatt tattagaatc
     1021 gcaaaagaga ataatgtcgt gacttggtaa ggagttttat gatgaaatca gaagtatatg
     1081 taattgcaga aattggtgta aaccataatg gaagtctaga tttagcttta gaaagtattg
     1141 ataaagctaa agaatgtgga gctaacgcgg ttaaatttca aacatttaaa acgaataagc
     1201 tagtttccaa aaaggctcca atggcaaaat atcagaagga
//
LOCUS       DAYYJC010000215         1238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000215 DAYYJC010000000
VERSION     DAYYJC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.227"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1182
                     /locus_tag="ACHA9N_002847"
     CDS             142..1182
                     /locus_tag="ACHA9N_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9086247.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          499 a          177 c          204 g          358 t
ORIGIN      
        1 attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc
       61 attttttatt tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa
      121 cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac
      181 aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg
      241 aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc
      301 aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt
      361 agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt
      421 ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat
      481 agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt
      541 gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc
      601 gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc
      661 caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta
      721 gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta
      781 gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta
      841 ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact
      901 attcataatg caaagaaaaa agctgaaata gaaagtgata tttcgatagt ttttgaagaa
      961 ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat
     1021 caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa
     1081 aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat
     1141 gcaagctccc caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg
     1201 attgttgctt catggagtaa cattaaaatt aaaaattt
//
LOCUS       DAYYJC010000216         1229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000216 DAYYJC010000000
VERSION     DAYYJC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.228"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACHA9N_002848"
     CDS             <1..189
                     /locus_tag="ACHA9N_002848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086248.1"
                     /translation="TFLCVAYFPLTIVFYLMVNLLKINTATTNGDQACFVTYFTIITT
                     LFYYSILLPYPSIPNHLW"
     gene            complement(231..1091)
                     /locus_tag="ACHA9N_002849"
     CDS             complement(231..1091)
                     /locus_tag="ACHA9N_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9086249.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          366 a          234 c          179 g          450 t
ORIGIN      
        1 acatttttgt gtgttgctta tttccctcta actatcgttt tctatttgat ggttaaccta
       61 cttaaaataa atactgcaac gacaaacgga gatcaagcat gctttgtaac gtattttacg
      121 ataattacga ctttatttta ttactctatc ttgctgccat acccgtctat ccctaatcat
      181 ctttggtgaa ttttttcaaa gttagcataa caaaaataag tattttttcc ttagttaatc
      241 aatagtatca tttttttcat atgatttaat tcatcaatta attttttgtt tttattaatt
      301 ttagcaaaag ctagtgcatc ttgaatcaat tcgagcactt ctgccttcga cttattttta
      361 gcctgtgcgt tcttcgctaa ttgggcagca gctcgtgcta agtaataagt caaatggtta
      421 tccgaacaaa tatcgattgc gtaatttagt agtgcatcac ttgtttccaa atcaccgtct
      481 tgcgcaaaaa aaacaccaca atgaaagacg atatttaaca cccgccagat atcttctgtt
      541 gtacgaatcg gatacgtata aattttttca aacactcgat tgaaataaac ttctgctttt
      601 tccaactctt tttctctagc atagaccatt ccaatacctg tatatgccaa taaacgaaac
      661 aacgtgttgg ctgtttctgc catcaacaac tgatcaaaat ggaacaacac atccgtaatt
      721 ggtgcattcg taaaaatcat ttcaaatcct tttagatagt agtaacgtgc aatcagctca
      781 gactcgtcaa ttaccttggg atcaatggct gctaaaatct gtgtcaattg ttcatattca
      841 ctcgtaatca gtgaaaactc tgctttttct agctgttcat caagttgtgt attgatcacg
      901 ttcatcgaag aaaaaagttc aactaacgga agattgattc gattacataa tttgattaaa
      961 atttttaacg aagggacttg accattgttc tcaaatttac ttaatgttgc ttgtgtacaa
     1021 attccttcag acacttcttt ttgagataag ttggcagcct ttcttgccgt aataaatttt
     1081 tcaatgttca ttattttctc tccaatcaga cctcacacga aaatcatttc tgtctcgatt
     1141 ttagcatgtt ttctttggga atgctttagg ttctgttgca aagttttaaa taaagaataa
     1201 agtcccttac ggtatctatg atttaagct
//
LOCUS       DAYYJC010000217         1220 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000217 DAYYJC010000000
VERSION     DAYYJC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.229"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1220
                     /locus_tag="ACHA9N_002850"
     CDS             <1..>1220
                     /locus_tag="ACHA9N_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9086250.1"
                     /translation="SYETLQKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNG
                     KKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASL
                     LTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDK
                     MSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLP
                     NAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINH
                     YEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSL
                     LAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKS
                     FENMRIRIFLINQL"
BASE COUNT          402 a          244 c          233 g          341 t
ORIGIN      
        1 tctcttacga gacccttcag aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta
       61 ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga tggaaacggg aaagcaatta
      121 tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga acaatacaat catatgcctt
      181 tggttatgcg cctaaaaaag caacgctata cctgtaaaaa ctgtcgcacc cattggacgg
      241 ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa tcatgttaga tataaaattg
      301 cttctttact gactgaaaaa gtatctttat cttttattgc gaaaagctgt caggtatctt
      361 tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta tttaccaaag caatctaaga
      421 agatcctccc cagagtattg atggttgacg aatttcgttc gcatgcttcc atagaagata
      481 agatgagctt tatttgcgcg gatggcgaaa caggaaaatt aatagatgtt ttgcctacgc
      541 gtaaattacc tcgattaaca agctatttct taggttgtac caatccagaa gaagtagaat
      601 tcttggtgac agacatgaac gccgcctact tccagctcac caaacgtgtt ctgccaaatg
      661 cgaaagtcgt gattgatcgg tttcatattg tcaaacacat gaatcaagcg ttcaatgagt
      721 tgcgtatccg tgaaatgaat gaacttcgta aagccggaca gaaaagccag gcagaaaaac
      781 tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca
      841 aaacatggaa aagtttccga gcaccaaaat acccatttct tactgaagca atgatgattg
      901 atcgattgct tgagttttct ccgcccctaa aggaggcgta tccctttttt catgaattag
      961 ttgaagcctt tcgagacaaa gaccctgact tatttttctc cttattggca gaacttcccg
     1021 aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct tctgacctat gaagaaggca
     1081 tcaccaacgc aatgatctat ccttattcca atggaaaaat agaagcgaag aatacccaca
     1141 taaagacaat gaaacgagta tcctacggat ttaaatcatt tgagaacatg agaattagaa
     1201 tctttttgat caatcaatta
//
LOCUS       DAYYJC010000218         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000218 DAYYJC010000000
VERSION     DAYYJC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.230"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1084
                     /locus_tag="ACHA9N_002851"
     CDS             <1..1084
                     /locus_tag="ACHA9N_002851"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9086251.1"
                     /translation="KKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSI
                     KNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYY
                     DRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLS
                     NSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALR
                     TAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFI
                     RPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKML
                     DYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          477 a          143 c          197 g          388 t
ORIGIN      
        1 taaaaagatt agttgtgtaa aaaaagatgg tacaagattc tctcaagaat atgacaaagt
       61 tatattagct acaggttcaa agcaaattat gctagatata ccggggaata atttagctaa
      121 tattttttcg ataaagaatt ttccaaatgc aaaagagatt tttaaagctt tggatgatag
      181 tactattaaa aaagtaggaa ttattggagc aggatatatt ggagttgaaa ttgctgaagc
      241 cataagaaaa agagggaaag aagtatatct tttcgatgtt gcagatagag tgttgtcgac
      301 ttattacgac cgtagttttt cagataaagt tgaagaaata ctatcaaaaa atggaataca
      361 tctttgtcta tcagaaagtc ctaaaaaata tttaggaaga aaaaagattg aaaaaatagt
      421 cactacaagt ggtacccaat actctatgga tatggtcgta caagctatag gatttctatc
      481 taatagtcca attggggaaa aggaactctt gagagatgtt agtggagcat atctaactaa
      541 tgaataccag caaacaaata taaaagatat atatgctatt ggggattgtg ctacaacttt
      601 ttttacgtcc attaatagaa gtagtgttga tttttctatt tctaatgctc taagaacagc
      661 ttatattgca actcaacata ttaacaatca agattttaca ccttctggtt cccaatttac
      721 aaatggcttt tctatattta attacaattt ttacgcagca ggtttgaatt taaaaacagc
      781 acgattatta agtgagtcta tagattatat agaaatagaa gatttcatta gacctgcttt
      841 tttgagaaaa aatagtcgtg taaaattaag aattagcttt gaaaaaaata caaagagaat
      901 agtaggtgta caactttgta gtcaagaaga tttatctgga ctattatcaa tgttttctct
      961 tgctatagaa gaacaaatta cattagacaa attgaaaatg ttagattatt tatttcatcc
     1021 tagttttagc caaccctata attttatttt aaaggcagta acaggattaa acattgaaac
     1081 ataatcgtta ctaaatgaaa tcaagaaata tactgtatta ctcaattcat tgaaaatatt
     1141 tatcataatt tttaatagaa aacactatta aagagaaaaa agagaggtaa atcaaatgaa
     1201 aaaat
//
LOCUS       DAYYJC010000219         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000219 DAYYJC010000000
VERSION     DAYYJC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.231"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1205
                     /locus_tag="ACHA9N_002852"
     CDS             <1..>1205
                     /locus_tag="ACHA9N_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM9086252.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          399 a          234 c          232 g          340 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aatta
//
LOCUS       DAYYJC010000220         1203 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000220 DAYYJC010000000
VERSION     DAYYJC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.232"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..160)
                     /locus_tag="ACHA9N_002853"
     CDS             complement(<1..160)
                     /locus_tag="ACHA9N_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9086253.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
     gene            369..581
                     /locus_tag="ACHA9N_002854"
     CDS             369..581
                     /locus_tag="ACHA9N_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086254.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            743..>1203
                     /locus_tag="ACHA9N_002855"
     CDS             743..>1203
                     /locus_tag="ACHA9N_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFM9086255.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKST"
BASE COUNT          436 a          181 c          185 g          401 t
ORIGIN      
        1 attcttgcac ccaacgataa atcgtcgtat gagaaacgtt aatgccacga tcatataaga
       61 tttcttgaac ttcacgatag ctaaggttat aacgaagata gtagcccacg gctacaataa
      121 tcacatcctg ctgaaattgc tttcctttaa aatgattcat cgtcattcct cctgctatct
      181 ttttctatta ttctacctta tttgatagta gatttaaaac tttgcaacag aaccctaaaa
      241 acactatata ttccgaaaag tgagtatctc cattcataaa ataatcaaca tatttaaaaa
      301 atctaattag cttttttagt caatctaaag ttctttattc tatgatataa tgttactgta
      361 gaaagtttgt ggcggtttgg caaatccaat tgaaggagcg tggtgcctat gggcttatta
      421 gcttatcgga cgtactctgt agaaaggaaa tgcctgattt gttctcattg gatcaaatca
      481 ttgctattat cggagcagca gcagtagtta ttactgccgt agcgaaagct agcgattcga
      541 ttttagcttg gttaaagttc aaaaaagaac aaaaaaaata gagccgtctc aacttttggc
      601 gaggtagacg agctctaaag ctttatttac atgccatccg ctttgaacgg actacactgg
      661 gcatgtgaca gcatgccctt tcatttatat tatatctcat tcttttatat aatgaaagta
      721 aataaatcat ggaggataaa atatggataa atttaataga ttaacattaa taaatcagtt
      781 tgaaatttta aaagctttaa atcctaacga agaaaaatac tatgctgaaa aaatagaaat
      841 actaacagaa ggttacgaat atcactatag tgaaatattt gaaaatattt ctgatccatt
      901 gccagaagaa gattcgcggt ttgttctaga catattaaat atgtatagag atattacttt
      961 tagtaaaaat aaattaaaag atataaattc aattgataat tattatatac aatttaaagg
     1021 ttttgacttt aatagctcta ccgaatttaa gttagcactt tatgcacaat tttttattga
     1081 aaaattaaat aggtttcaag aaatagttga agatagtgat tttaacactt ttaatagcca
     1141 taaaccgatg agaggtactt atataaaatt tctcgaaaat tataatgatt taaaatctac
     1201 caa
//
LOCUS       DAYYJC010000221         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000221 DAYYJC010000000
VERSION     DAYYJC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.233"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(305..661)
                     /locus_tag="ACHA9N_002856"
     CDS             complement(305..661)
                     /locus_tag="ACHA9N_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9086256.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="ACHA9N_002857"
     CDS             complement(675..1085)
                     /locus_tag="ACHA9N_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9086257.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          384 a          222 c          167 g          420 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acg
//
LOCUS       DAYYJC010000222         1193 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000222 DAYYJC010000000
VERSION     DAYYJC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.234"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHA9N_002858"
     CDS             <1..1076
                     /locus_tag="ACHA9N_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9086258.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAYYJC010000223         1151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000223 DAYYJC010000000
VERSION     DAYYJC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.235"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACHA9N_002859"
     CDS             <1..153
                     /locus_tag="ACHA9N_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFM9086259.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            207..575
                     /locus_tag="ACHA9N_002860"
                     /pseudo
     CDS             207..575
                     /locus_tag="ACHA9N_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..944
                     /locus_tag="ACHA9N_002861"
     CDS             681..944
                     /locus_tag="ACHA9N_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9086260.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1151
                     /locus_tag="ACHA9N_002862"
     CDS             944..>1151
                     /locus_tag="ACHA9N_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9086261.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVY"
BASE COUNT          438 a          149 c          231 g          333 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat a
//
LOCUS       DAYYJC010000224         1142 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000224 DAYYJC010000000
VERSION     DAYYJC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.236"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..922)
                     /gene="xerC"
                     /locus_tag="ACHA9N_002863"
     CDS             complement(17..922)
                     /gene="xerC"
                     /locus_tag="ACHA9N_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9086262.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          378 t
ORIGIN      
        1 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
       61 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
      121 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
      181 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
      241 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
      301 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
      361 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
      421 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
      481 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
      541 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
      601 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
      661 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
      721 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
      781 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
      841 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
      901 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
      961 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
     1021 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
     1081 gggctctata atttatggga ctgatgaatc agaattgatt cttcagcccc attttcattt
     1141 ta
//
LOCUS       DAYYJC010000225         1105 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000225 DAYYJC010000000
VERSION     DAYYJC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.237"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA9N_002864"
     CDS             <1..1087
                     /locus_tag="ACHA9N_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9086263.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAYYJC010000226         1076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000226 DAYYJC010000000
VERSION     DAYYJC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.238"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            399..1034
                     /locus_tag="ACHA9N_002865"
                     /pseudo
     CDS             399..1034
                     /locus_tag="ACHA9N_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          371 a          190 c          191 g          324 t
ORIGIN      
        1 taaaatattt tttatattac aaaagtagtg tttcaaagtt agtagagaaa atgtagttat
       61 atatttaaat ttttaccaga aagaaatgaa caccttcaat tatctaaatc tcgaaaatgg
      121 ttagatgtaa tcatcaggaa aaacaatctt aaacgaatta ctattcatgg tcttagacac
      181 acacatgcta gcttgctttt agcaagctag tgcaaatatt aaagatgtgc aagagcgttt
      241 aggccactct tctattcaaa tcattatgga tctatatatt catattacag acaaaagaaa
      301 agaagaaacc gtagcgcaat tcgcaaaata taggctctat aatttatggg actgatgaat
      361 caaaattgat tcttcagtcc cattgttatt ttaggtatat gattcgtaca ttgaaagagt
      421 tgaaaaacta tcttccgaag ccgtcaaaaa ggaccctgcc aaaagtattg atggtggatg
      481 aatttcgttc gcacgcttca attgtggata aaatgagctt tatttgtgcg gatggcgaga
      541 ctggaaaact cattgatatt ttgcctacac gaaaattgtc tcgattaaca cactatttta
      601 gtcgctgtgc aaatcctgaa gcggtggaat atttagtgac agatatgaac gccgcctact
      661 tccaactgac aaaacgtgtc ttaccaaatg cgaaagtcgt cattgattgt tttcatatca
      721 tcaaacattt gaatcaggcg ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc
      781 agttagggca acgacgggaa gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa
      841 accgtcaaaa aatcaatcac tatgattaca aaacatggcg cagttttcgt gcacctaaat
      901 acccgctact cactgaagca atgatgattg atcgattgct ggcattttca acgcctttaa
      961 aggacgccta tacttttttc catgagctga ccgaagcctt tagagacaag gaatcagact
     1021 tattttttac cttatattat ttgtcaaatt aagcgagaca aaactttcca tttacg
//
LOCUS       DAYYJC010000227         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000227 DAYYJC010000000
VERSION     DAYYJC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.239"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACHA9N_002866"
     CDS             complement(37..>1062)
                     /locus_tag="ACHA9N_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9086264.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAYYJC010000228         1051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000228 DAYYJC010000000
VERSION     DAYYJC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.240"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..964
                     /locus_tag="ACHA9N_002867"
     CDS             17..964
                     /locus_tag="ACHA9N_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9086265.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          337 a          163 c          229 g          322 t
ORIGIN      
        1 tatcggaggt aaaaggatga gctgttatct tatcaccggt ggggctggct tcattggttc
       61 taccttagcc aatcatttag gagaagaaca tgtggtaatc gtcgtcgatg acttatcaat
      121 gggaaaagta gaaaatcttg atatgacgaa aaatatcact tttattgagg gagatgtcgc
      181 tgagaaatcc ttgatggaag agatcatgaa agctaataaa tttgattata tctttcattt
      241 agcagcagtt gctagtgtgg cagattcagt tgcccgacca gtagaaacac atcgtgtgaa
      301 ttttgaaagt gtattaatga ttttagaatt gatccgcaag tatcaacctg atttaaaacg
      361 gatcgtgttt agttcttcag cggctgttta tggggatgaa ccgactttac caaaaaaaga
      421 agagtccgtg attcgcccat tgacgcctta tgcggttgat aaattcgctg cagaacaata
      481 tgtgctagat tattgtcatt tatatgatgt acccggtagc gcagttcgtt ttttcaatgt
      541 ctatggtcca aatcaaaatc cgaattctcc atacagtggt gtgatttcta ttctcgtaga
      601 tcgctataag aaacaaatcg taggagaagc aacttcattt acattatttg gtgatggaag
      661 ccagtctaga gactttgttt atatagatga tgtaatccaa gcattacttt tagttgcgaa
      721 tgaggaagct gcactgggga aacaattcaa tgttggaacg ggtaaagcaa ccacactttt
      781 ggaattgatt catgcaatca atgaaattct cggtgtagaa ttgacacttg aatacaaaga
      841 agagcgttcg ggtgatatcc atgactctct agcagatatt actaagatcc agtcaattgg
      901 ctatcaaccg aaatatggta ttttaagtgg aatgactaat tatttgaaaa ctgaaatcaa
      961 ttaaaacata ctaagataag attagagggt aaattcaggc tctttgtcaa taaggactga
     1021 tgagttttgt aaaatcaaat ctgggataga a
//
LOCUS       DAYYJC010000229         1037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000229 DAYYJC010000000
VERSION     DAYYJC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.241"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHA9N_002868"
     tRNA            complement(20..91)
                     /locus_tag="ACHA9N_002868"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHA9N_002869"
     tRNA            complement(113..200)
                     /locus_tag="ACHA9N_002869"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHA9N_002870"
     tRNA            complement(225..298)
                     /locus_tag="ACHA9N_002870"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHA9N_002871"
     tRNA            complement(312..382)
                     /locus_tag="ACHA9N_002871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHA9N_002872"
     tRNA            complement(384..459)
                     /locus_tag="ACHA9N_002872"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHA9N_002873"
     tRNA            complement(478..551)
                     /locus_tag="ACHA9N_002873"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHA9N_002874"
     tRNA            complement(564..653)
                     /locus_tag="ACHA9N_002874"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHA9N_002875"
     tRNA            complement(677..750)
                     /locus_tag="ACHA9N_002875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHA9N_002876"
     tRNA            complement(764..837)
                     /locus_tag="ACHA9N_002876"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHA9N_002877"
     tRNA            complement(865..937)
                     /locus_tag="ACHA9N_002877"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHA9N_002878"
     tRNA            complement(957..1030)
                     /locus_tag="ACHA9N_002878"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAYYJC010000230          995 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000230 DAYYJC010000000
VERSION     DAYYJC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.242"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACHA9N_002879"
     CDS             complement(26..913)
                     /locus_tag="ACHA9N_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9086266.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DAYYJC010000231          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000231 DAYYJC010000000
VERSION     DAYYJC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.243"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACHA9N_002880"
     rRNA            complement(<1..>994)
                     /locus_tag="ACHA9N_002880"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAYYJC010000232          985 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000232 DAYYJC010000000
VERSION     DAYYJC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.244"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACHA9N_002881"
     CDS             <1..704
                     /locus_tag="ACHA9N_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9086267.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataa
//
LOCUS       DAYYJC010000233          959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000233 DAYYJC010000000
VERSION     DAYYJC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.245"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACHA9N_002882"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACHA9N_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACHA9N_002883"
     CDS             complement(570..>959)
                     /locus_tag="ACHA9N_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086268.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAYYJC010000234          905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000234 DAYYJC010000000
VERSION     DAYYJC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.246"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACHA9N_002884"
     CDS             265..888
                     /locus_tag="ACHA9N_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9086269.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DAYYJC010000235          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000235 DAYYJC010000000
VERSION     DAYYJC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.247"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACHA9N_002885"
     CDS             <1..888
                     /locus_tag="ACHA9N_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9086270.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAYYJC010000236          886 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000236 DAYYJC010000000
VERSION     DAYYJC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.248"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..145)
                     /locus_tag="ACHA9N_002886"
     tRNA            complement(73..145)
                     /locus_tag="ACHA9N_002886"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:His,seq:gtg)
     gene            complement(148..221)
                     /locus_tag="ACHA9N_002887"
     tRNA            complement(148..221)
                     /locus_tag="ACHA9N_002887"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(187..189),aa:Trp,seq:cca)
     gene            complement(228..308)
                     /locus_tag="ACHA9N_002888"
     tRNA            complement(228..308)
                     /locus_tag="ACHA9N_002888"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Tyr,seq:gta)
     gene            complement(310..385)
                     /locus_tag="ACHA9N_002889"
     tRNA            complement(310..385)
                     /locus_tag="ACHA9N_002889"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Phe,seq:gaa)
     gene            complement(421..493)
                     /locus_tag="ACHA9N_002890"
     tRNA            complement(421..493)
                     /locus_tag="ACHA9N_002890"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(458..460),aa:Asp,seq:gtc)
     gene            complement(503..575)
                     /locus_tag="ACHA9N_002891"
     tRNA            complement(503..575)
                     /locus_tag="ACHA9N_002891"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Val,seq:tac)
     gene            complement(591..662)
                     /locus_tag="ACHA9N_002892"
     tRNA            complement(591..662)
                     /locus_tag="ACHA9N_002892"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(627..629),aa:Glu,seq:ttc)
     gene            complement(677..766)
                     /locus_tag="ACHA9N_002893"
     tRNA            complement(677..766)
                     /locus_tag="ACHA9N_002893"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Ser,seq:gga)
     gene            complement(806..879)
                     /locus_tag="ACHA9N_002894"
     tRNA            complement(806..879)
                     /locus_tag="ACHA9N_002894"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(843..845),aa:Asn,seq:gtt)
BASE COUNT          248 a          243 c          203 g          192 t
ORIGIN      
        1 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
       61 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
      121 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
      181 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
      241 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
      301 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
      361 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
      421 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
      481 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
      541 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
      601 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
      661 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
      721 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
      781 tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac gacatcatga
      841 ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacat
//
LOCUS       DAYYJC010000237          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000237 DAYYJC010000000
VERSION     DAYYJC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.249"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACHA9N_002895"
     CDS             <1..>872
                     /locus_tag="ACHA9N_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9086271.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DAYYJC010000238          816 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000238 DAYYJC010000000
VERSION     DAYYJC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.250"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHA9N_002896"
     CDS             complement(<1..430)
                     /locus_tag="ACHA9N_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086272.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            complement(563..>816)
                     /locus_tag="ACHA9N_002897"
     CDS             complement(563..>816)
                     /locus_tag="ACHA9N_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086273.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          231 a          155 c          116 g          314 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcattttgta gtgaccccca aaagttagac ttttttggag tggagaaatc cactcttttt
      541 ttcatgctct aaaatgatat ttttacacaa ccgtaacggt tgttttacga aattggacgg
      601 gactttgcca gtttaatttc tgttttatcc gttcattatt gtagtaatag atatagctat
      661 cgatggctga ttttaattcc tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt
      721 ttaataagcc aaaaaaattc tccataagtg aattatctaa gcagttccct ttccgagaca
      781 tgctttgaaa gattttgtac tccttcaatt tgtttc
//
LOCUS       DAYYJC010000239          762 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000239 DAYYJC010000000
VERSION     DAYYJC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.251"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..>762
                     /locus_tag="ACHA9N_002898"
     CDS             19..>762
                     /locus_tag="ACHA9N_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFM9086274.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          252 a          140 c          156 g          214 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac caatgtcgct
      301 taccgctggt tcttaggcta ctccttcgag gagaaaatcc cccatttctc aacttttgga
      361 aagaattatg ttcgccgatt tagagaaacg acggtgtttg aggatatttt tgcctatatt
      421 ttagaacaag cagttaaagc aggttttgta acagaggata atttgtatct ggattctacc
      481 cacatcaaag ctaacgccaa taaacataag tttacgaaag aaatgaccca tgatgaagcg
      541 aaagcctatc aggatgaatt agaagatgag atcaatcgtc aacgaataga ggctggaaaa
      601 cgccctttta ctttggactt agaaaaggaa gtgaagctga aagaaagaaa aataagtaaa
      661 gctgatccgg aaagcggcta ctacgtcaaa ggcgaacgag aaaaacaatt tgcttattct
      721 gcccatactt catgcgacga caatggattt attcttagca ca
//
LOCUS       DAYYJC010000240          752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000240 DAYYJC010000000
VERSION     DAYYJC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.252"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>752)
                     /locus_tag="ACHA9N_002899"
     CDS             complement(91..>752)
                     /locus_tag="ACHA9N_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFM9086275.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DAYYJC010000241          733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000241 DAYYJC010000000
VERSION     DAYYJC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.253"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACHA9N_002900"
     rRNA            <1..>733
                     /locus_tag="ACHA9N_002900"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAYYJC010000242          732 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000242 DAYYJC010000000
VERSION     DAYYJC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.254"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..293
                     /locus_tag="ACHA9N_002901"
     CDS             99..293
                     /locus_tag="ACHA9N_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086276.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          301 a           94 c          125 g          212 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aa
//
LOCUS       DAYYJC010000243          712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000243 DAYYJC010000000
VERSION     DAYYJC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.255"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACHA9N_002902"
     CDS             complement(<1..446)
                     /locus_tag="ACHA9N_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9086277.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DAYYJC010000244          711 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000244 DAYYJC010000000
VERSION     DAYYJC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.256"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(602..675)
                     /locus_tag="ACHA9N_002903"
     tRNA            complement(602..675)
                     /locus_tag="ACHA9N_002903"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          249 a          124 c          118 g          220 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc a
//
LOCUS       DAYYJC010000245          703 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000245 DAYYJC010000000
VERSION     DAYYJC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.257"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          297 a          106 c          122 g          178 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgt
//
LOCUS       DAYYJC010000246          649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000246 DAYYJC010000000
VERSION     DAYYJC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.258"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACHA9N_002904"
     CDS             <1..>649
                     /locus_tag="ACHA9N_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9086278.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAYYJC010000247          635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000247 DAYYJC010000000
VERSION     DAYYJC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.260"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACHA9N_002905"
     CDS             complement(<1..270)
                     /locus_tag="ACHA9N_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086279.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACHA9N_002906"
     CDS             complement(276..>635)
                     /locus_tag="ACHA9N_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9086280.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAYYJC010000248          606 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000248 DAYYJC010000000
VERSION     DAYYJC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.261"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(275..466)
                     /locus_tag="ACHA9N_002907"
     CDS             complement(275..466)
                     /locus_tag="ACHA9N_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086281.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          200 a          119 c           85 g          202 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaaaaacaga
      541 aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca ataatatttt
      601 tctaac
//
LOCUS       DAYYJC010000249          600 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000249 DAYYJC010000000
VERSION     DAYYJC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.262"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..501)
                     /locus_tag="ACHA9N_002908"
     CDS             complement(<1..501)
                     /locus_tag="ACHA9N_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9086282.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVF"
BASE COUNT          261 a           88 c           68 g          183 t
ORIGIN      
        1 aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa ataaaccaat
       61 tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt caacaatcct
      121 atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag aatcaagcat
      181 tatctggaat actacataac catacccttt tatcccttca aatgaaaaca tatacgtcat
      241 aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata gtaatttatt
      301 taaaagcact atattagttt tcttcttttc ctttaatata aaataacttc cgattatagg
      361 tatagctgcg gaaacatcag taaataattc aacaaaaaaa gtgagtggta tcaataaaca
      421 tacaaaaaga tattttcttt tgttaagatc agtattagtc atataaaata agagaaaaaa
      481 gaaaaagttt cccacgaaca ttcttaatag ataagctttt gtatcgatag taatcaagag
      541 atcccctcct gattttagga tatcataaaa aaaattttag ttacactttt taaataatga
//
LOCUS       DAYYJC010000250          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000250 DAYYJC010000000
VERSION     DAYYJC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.263"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA9N_002909"
     tRNA            complement(65..150)
                     /locus_tag="ACHA9N_002909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA9N_002910"
     tRNA            complement(167..238)
                     /locus_tag="ACHA9N_002910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA9N_002911"
     tRNA            complement(250..322)
                     /locus_tag="ACHA9N_002911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA9N_002912"
     tRNA            complement(340..421)
                     /locus_tag="ACHA9N_002912"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA9N_002913"
     tRNA            complement(429..501)
                     /locus_tag="ACHA9N_002913"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA9N_002914"
     tRNA            complement(505..577)
                     /locus_tag="ACHA9N_002914"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYJC010000251          558 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000251 DAYYJC010000000
VERSION     DAYYJC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.265"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..532
                     /gene="spxA"
                     /locus_tag="ACHA9N_002915"
     CDS             134..532
                     /gene="spxA"
                     /locus_tag="ACHA9N_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9086283.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          197 a           87 c          110 g          164 t
ORIGIN      
        1 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
       61 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
      121 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
      181 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
      241 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
      301 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
      361 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
      421 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
      481 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
      541 tacgaaactt ggaaaaga
//
LOCUS       DAYYJC010000252          556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000252 DAYYJC010000000
VERSION     DAYYJC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.266"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA9N_002916"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACHA9N_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9086284.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..543)
                     /locus_tag="ACHA9N_002917"
     CDS             complement(349..543)
                     /locus_tag="ACHA9N_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086285.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          164 a          122 c           93 g          177 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatctcat ttaatttata
      541 catagaagac ctccag
//
LOCUS       DAYYJC010000253          527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000253 DAYYJC010000000
VERSION     DAYYJC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.267"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACHA9N_002918"
     CDS             <1..159
                     /locus_tag="ACHA9N_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9086286.1"
                     /translation="SFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANE
                     VLKIAEEK"
     gene            160..>527
                     /locus_tag="ACHA9N_002919"
     CDS             160..>527
                     /locus_tag="ACHA9N_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9086287.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNL"
BASE COUNT          199 a           62 c          103 g          163 t
ORIGIN      
        1 agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat gtatgagaag
       61 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
      121 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
      181 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
      241 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
      301 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
      361 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
      421 attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat atttgattta
      481 attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatct
//
LOCUS       DAYYJC010000254          526 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000254 DAYYJC010000000
VERSION     DAYYJC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.268"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>526
                     /locus_tag="ACHA9N_002920"
     CDS             <1..>526
                     /locus_tag="ACHA9N_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9086288.1"
                     /translation="VSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQR
                     SIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDF"
BASE COUNT          170 a          103 c           95 g          158 t
ORIGIN      
        1 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
       61 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      121 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt
      181 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      241 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      301 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      361 ttacgtttga ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata
      421 ttgatatata ttttgcggac cctggaacac cttctcaacg cccattaaac gagaattcta
      481 acgggattct gcgtcgtaat ggactgccga aatcaatgga ttttag
//
LOCUS       DAYYJC010000255          516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000255 DAYYJC010000000
VERSION     DAYYJC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.269"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..>516
                     /locus_tag="ACHA9N_002921"
     CDS             241..>516
                     /locus_tag="ACHA9N_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9086289.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          191 a           83 c           89 g          153 t
ORIGIN      
        1 agtttttttt cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt
       61 atcacgtcta aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa
      121 gcgcgacaac gttgtttaat atatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg
      181 catttatggt acactcaaat tgtatttccg acgaaagaaa acaaaggagt gtaaccataa
      241 atgacctaca aacatcttac catagacgaa ctgacaatga tagaatcata ttatcttcaa
      301 cataataagc cggttgaaat cgctaaccga atgggacgtg ctatacaaac tatttataat
      361 gtagtcaata agttcaagca aggcaagact gctcttgatt attggcacca gtataaagaa
      421 aataagaaaa aatgtggtag aaaagtcatt caattacctg ctcatgaagt agattacatt
      481 aaagagaaag tcactcttgg ttggacgcct gacgtc
//
LOCUS       DAYYJC010000256          499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000256 DAYYJC010000000
VERSION     DAYYJC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.271"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..491
                     /locus_tag="ACHA9N_002922"
     CDS             <1..491
                     /locus_tag="ACHA9N_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086290.1"
                     /translation="LFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKKDIETDLKN
                     DHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLESIFSDINS
                     LKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKL
                     RY"
BASE COUNT          214 a           48 c           73 g          164 t
ORIGIN      
        1 atttatttgc tattataggt gttattgcta acatactttt ttcgtgtttg aattttagat
       61 ttactcaaaa gaacaaaaag aacaaactca gtatagaaca aatgaaaaaa gacattgaaa
      121 ctgatttaaa aaatgaccat aaagaaagaa gaaaaattga agaatatact tctttaatgg
      181 aaaagtgtaa agcaactgat gcaattttta gctttttaag aaaaagtgat gagataatca
      241 cacgtgaaaa tatagattta gaaaatgaga ttagtaaact tgaatctatc ttttccgata
      301 taaattctct taagtattta atcgtcgaag aaaattcgga tagcttttta gaatttgaaa
      361 aatatctaac agatcttata gataatagaa ataatgaatt aatgagacaa aacattgcaa
      421 gattccttac taaattagtt tctgaatgga aaagtattat ttctcaaaat atggataaat
      481 taagatatta aggatgtta
//
LOCUS       DAYYJC010000257          485 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000257 DAYYJC010000000
VERSION     DAYYJC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.272"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACHA9N_002923"
     CDS             complement(62..259)
                     /locus_tag="ACHA9N_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9086291.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAYYJC010000258          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000258 DAYYJC010000000
VERSION     DAYYJC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.275"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACHA9N_002924"
     CDS             <1..>452
                     /locus_tag="ACHA9N_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086292.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAYYJC010000259          444 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000259 DAYYJC010000000
VERSION     DAYYJC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.276"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..281
                     /locus_tag="ACHA9N_002925"
     CDS             78..281
                     /locus_tag="ACHA9N_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFM9086293.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            281..>444
                     /locus_tag="ACHA9N_002926"
     CDS             281..>444
                     /locus_tag="ACHA9N_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9086294.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILV"
BASE COUNT          179 a           48 c           86 g          131 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      421 aaaatatcct gaaattcttg taaa
//
LOCUS       DAYYJC010000260          425 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000260 DAYYJC010000000
VERSION     DAYYJC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.277"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>425
                     /locus_tag="ACHA9N_002927"
     CDS             <1..>425
                     /locus_tag="ACHA9N_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9086295.1"
                     /translation="KLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIR"
BASE COUNT          158 a           77 c           79 g          111 t
ORIGIN      
        1 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
       61 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      121 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      181 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      241 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      301 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      361 cgcaataaat tctatcgaag tttacgcact gcctctacca cgattaaagg catggaagcc
      421 attcg
//
LOCUS       DAYYJC010000261          423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000261 DAYYJC010000000
VERSION     DAYYJC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.278"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..423
                     /locus_tag="ACHA9N_002928"
                     /pseudo
     CDS             2..423
                     /locus_tag="ACHA9N_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          171 a           75 c           81 g           96 t
ORIGIN      
        1 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
       61 aacggcttca gcatttgaca aatgggtaaa gcaatctaaa acgagtggtt cattcaaaga
      121 aaaagataat cttacgcctg aacaaaaaga attgttagaa ctacgtaaaa gaaaccagca
      181 attagaaatg gaaaatgata ttttaaagca agcagcgctg atattcggac gaagagacaa
      241 gtaatcgatg cgaataagca tctttaccct atatcagcga tgtgcagaat attaggtcta
      301 tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga acttgaagaa
      361 gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa aataaaaaaa
      421 gcc
//
LOCUS       DAYYJC010000262          401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000262 DAYYJC010000000
VERSION     DAYYJC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.281"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DAYYJC010000263          375 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000263 DAYYJC010000000
VERSION     DAYYJC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.283"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA9N_002929"
     CDS             complement(<1..132)
                     /locus_tag="ACHA9N_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9086296.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAYYJC010000264          373 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000264 DAYYJC010000000
VERSION     DAYYJC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.284"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..272
                     /locus_tag="ACHA9N_002930"
                     /pseudo
     CDS             162..272
                     /locus_tag="ACHA9N_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          125 a           60 c           42 g          146 t
ORIGIN      
        1 acatctttca gcattttact atatttggta tatagtgcta aggttcatat tcgattgaca
       61 agagaatata aattgatcta tttctatttc ctaagttgag taaacataac tttgcccttt
      121 ataaatatct tccctctctc tcttctctct atattttata tttgaataat catatcaagg
      181 tattccaacg aaatgccaat ggatttcgaa gcttttataa ctttaaatta cggatcatga
      241 tttggcatgg ggaatcatta ctaataaaat aaaaaaatga acagaaagaa aacagttctt
      301 tctgttcacc aacttcattt gacaaatatc ctatttagct tattttatag ctttttatat
      361 acactttttt aaa
//
LOCUS       DAYYJC010000265          353 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000265 DAYYJC010000000
VERSION     DAYYJC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.286"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHA9N_002931"
     CDS             complement(<1..321)
                     /locus_tag="ACHA9N_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9086297.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           56 c           66 g          134 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaattggtt gtc
//
LOCUS       DAYYJC010000266          341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000266 DAYYJC010000000
VERSION     DAYYJC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.287"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACHA9N_002932"
     CDS             <1..>341
                     /locus_tag="ACHA9N_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9086298.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAYYJC010000267          331 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000267 DAYYJC010000000
VERSION     DAYYJC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.289"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>331
                     /locus_tag="ACHA9N_002933"
     CDS             <1..>331
                     /locus_tag="ACHA9N_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFM9086299.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAYYJC010000268          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000268 DAYYJC010000000
VERSION     DAYYJC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.290"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA9N_002934"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA9N_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086300.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYJC010000269          308 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000269 DAYYJC010000000
VERSION     DAYYJC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.292"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACHA9N_002935"
     CDS             complement(76..>308)
                     /locus_tag="ACHA9N_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9086301.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAYYJC010000270          292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000270 DAYYJC010000000
VERSION     DAYYJC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.294"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..185
                     /locus_tag="ACHA9N_002936"
     CDS             <1..185
                     /locus_tag="ACHA9N_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086302.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DAYYJC010000271          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000271 DAYYJC010000000
VERSION     DAYYJC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.295"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACHA9N_002937"
     CDS             <1..>291
                     /locus_tag="ACHA9N_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9086303.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           86 a           55 c           58 g           92 t
ORIGIN      
        1 agctcgtcaa acaatcgaag agactttttc ctgaaatcaa ttgtgttgtc gctgatgctg
       61 ggtataagac acctaagttt gttcactttt tgactcattt gaaccttcga ccttgtttgc
      121 cttattcaag gcccaaaggg aaaaaggggc tattatcaaa aaatgagttt ctctatgacg
      181 agtattttga ttgttatatt tgtccgcaag atcaaatgct cgctttttca acggttactc
      241 gagaagggta ccgagaatac aaatccaatc caaaagaatg tgtgaactgt c
//
LOCUS       DAYYJC010000272          291 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000272 DAYYJC010000000
VERSION     DAYYJC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.296"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..>291
                     /locus_tag="ACHA9N_002938"
     CDS             131..>291
                     /locus_tag="ACHA9N_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9086304.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          117 a           49 c           42 g           83 t
ORIGIN      
        1 atattgaaaa atacaaatat atacgtatat tataaagcgc cgattgtaaa attaagctag
       61 acaactaaaa cagtcatttg tgctacactc aaaatagttc cgaccaaaga attataagga
      121 gtgagtgcaa ataacctata cctatcttac aacggatgaa cttgtaataa tagagtctta
      181 tttgaaaatt aaccaatctg ttgctaaaac tgcgcattgc ttgaatcgtt caagtcaaac
      241 gatccataaa gtatatctgt tcttcaagca aagaaaatca gccttagaat a
//
LOCUS       DAYYJC010000273          281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000273 DAYYJC010000000
VERSION     DAYYJC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.297"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..281
                     /locus_tag="ACHA9N_002939"
                     /pseudo
     CDS             1..281
                     /locus_tag="ACHA9N_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          109 a           56 c           55 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca g
//
LOCUS       DAYYJC010000274          270 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000274 DAYYJC010000000
VERSION     DAYYJC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.denovo.299"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>270
                     /locus_tag="ACHA9N_002940"
     CDS             <1..>270
                     /locus_tag="ACHA9N_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9086305.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DAYYJC010000275        14622 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.064, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000275 DAYYJC010000000
VERSION     DAYYJC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.064"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..982
                     /locus_tag="ACHA9N_002941"
     CDS             86..982
                     /locus_tag="ACHA9N_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9086306.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1132..2073)
                     /locus_tag="ACHA9N_002942"
     CDS             complement(1132..2073)
                     /locus_tag="ACHA9N_002942"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9086307.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2199..3320
                     /locus_tag="ACHA9N_002943"
     CDS             2199..3320
                     /locus_tag="ACHA9N_002943"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9086308.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3578..5080
                     /gene="eat(A)"
                     /locus_tag="ACHA9N_002944"
     CDS             3578..5080
                     /gene="eat(A)"
                     /locus_tag="ACHA9N_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9086309.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5147..5674
                     /locus_tag="ACHA9N_002945"
     CDS             5147..5674
                     /locus_tag="ACHA9N_002945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9086310.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5885..6946
                     /locus_tag="ACHA9N_002946"
     CDS             5885..6946
                     /locus_tag="ACHA9N_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9086311.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6949..7845
                     /gene="murQ"
                     /locus_tag="ACHA9N_002947"
     CDS             6949..7845
                     /gene="murQ"
                     /locus_tag="ACHA9N_002947"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9086312.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7859..9841
                     /locus_tag="ACHA9N_002948"
     CDS             7859..9841
                     /locus_tag="ACHA9N_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9086313.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9906..10352)
                     /gene="nrdI"
                     /locus_tag="ACHA9N_002949"
     CDS             complement(9906..10352)
                     /gene="nrdI"
                     /locus_tag="ACHA9N_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9086314.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10563..11987
                     /locus_tag="ACHA9N_002950"
     CDS             10563..11987
                     /locus_tag="ACHA9N_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9086315.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12168..12941)
                     /locus_tag="ACHA9N_002951"
     CDS             complement(12168..12941)
                     /locus_tag="ACHA9N_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9086316.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12938..13843)
                     /locus_tag="ACHA9N_002952"
     CDS             complement(12938..13843)
                     /locus_tag="ACHA9N_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9086317.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13997..14317)
                     /locus_tag="ACHA9N_002953"
     CDS             complement(13997..14317)
                     /locus_tag="ACHA9N_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9086318.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4669 a         2608 c         3099 g         4246 t
ORIGIN      
        1 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
       61 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
      121 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
      181 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
      241 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
      301 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
      361 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
      421 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
      481 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
      541 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
      601 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
      661 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
      721 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
      781 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
      841 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
      901 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
      961 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     1021 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     1081 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     1141 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     1201 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     1261 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     1321 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     1381 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     1441 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     1501 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     1561 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     1621 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     1681 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     1741 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     1801 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     1861 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     1921 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     1981 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     2041 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     2101 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     2161 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     2221 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     2281 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     2341 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     2401 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     2461 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     2521 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     2581 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     2641 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     2701 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     2761 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     2821 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     2881 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     2941 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     3001 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     3061 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     3121 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     3181 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     3241 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     3301 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     3361 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     3421 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     3481 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     3541 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     3601 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     3661 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     3721 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     3781 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     3841 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     3901 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     3961 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     4021 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     4081 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     4141 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     4201 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     4261 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     4321 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     4381 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     4441 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     4501 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     4561 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     4621 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     4681 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     4741 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     4801 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     4861 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     4921 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     4981 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     5041 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     5101 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     5161 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     5221 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     5281 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     5341 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     5401 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     5461 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     5521 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     5581 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     5641 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     5701 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     5761 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     5821 ttgtttcttt agatatatac tctagtcata gaaatgaaga cggatacaga aaaagaggga
     5881 taatatgttt ggcttttcaa tatttatgaa cgattctttg tcagaagaga agaaacagta
     5941 tatccgccaa atggcatcca atggctttgt cggtattttc acttccatgc atataccaga
     6001 agatgatgtt tcggcatatc aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa
     6061 taaactagaa ttgatggtcg atatttcagg agaggcattg gaacgagcgg gtttttcatt
     6121 agaagatcta acgcctttga aagcaattgg tgtaacaggt ttaagaatgg attatcatat
     6181 ttccaatcag cagattgccg actggtcaca tcaattgaaa atcagtttga atgctagcac
     6241 gatcacgcct aaagatatcg atgaattgaa agaagcagaa gctgatttct cacagattga
     6301 agcttggcat aattattatc cacgtcccga aacagggctg gacaaagaat ggtatcagaa
     6361 aaaaaatcaa tggctcaaaa ttcaaggtct ccttgtccaa ggttttgtac caggagatac
     6421 agagttgaga ggcccactat atcaaggatt gccaactttg gaagaacatc gagggatcca
     6481 tcctcttgca ggcgctctag atctattagc aagtaacacg gatattgttt acatcggaga
     6541 tgcaggtctg tcagaaaacg ttcaggagca atttgcttct ttccaaaaag aacagacagt
     6601 cttgctccat acggaacctg tagatgaaga attttacgaa tatatattag ggaagcatac
     6661 gaatcggcaa gacgatgcaa gagatgtcat acgaagcgta gatgcacgtt ttcgagagat
     6721 tccgcctatc ccagcaagaa atacagctac gcgtatgaaa gggtctatta ctttagataa
     6781 cgaaaagtat ttgcgctata tgggtgagat ccaattaacc aaatatgatt tgccagcgga
     6841 tgaaaaagtg aatgtagtag ctaaagttat caaagaagag ctaccattga tcaaccaaat
     6901 caaagctggt atgaactatc aatttataag aaaagaagga agataaaaat gagcaaaatc
     6961 aatttagaga acctaacaac ggaacgcaga aaccaagaaa cttttggatt agatgagatg
     7021 agtatttctc aagccgttca actaatgaac aaagaagatc agaaagtggc cgctgcggtt
     7081 gaaaaagaac tgacaagtat cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa
     7141 ggtggacgtc tgatttatat gggagccgga acaagtggaa gactaggtgt gttagatgct
     7201 gctgaatgcg taccgacatt cggtgtagaa ccggaaatgg tcataggtct aattgctggt
     7261 ggagaagaag caatgactgt agctgtcgaa ggggccgaag attcttatga gctagggcaa
     7321 gaagatctaa tcaagctgaa tttgacaaaa aatgatatag ttattggtat cgcagctagc
     7381 ggtcgtacac catatgtgat cggtggatta gattacgcaa aatcgatcgg tgctgtcaca
     7441 ggaacgattt cttgcaataa acaagccgaa atcagtaaac acgcggattt cccaatcgaa
     7501 gtcgatgcag gtccagaatt tttgacaggc tctactcgat tgaagtcagg aacagcacaa
     7561 aaacttatct tgaatatgat ttctactatc agtatgattg gtataggtaa agtattcaat
     7621 aacttgatgg tcgatgtcaa accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc
     7681 atcatgcaag cgacagaagc tgattacgaa acagctgaaa agtactttga agaagcagat
     7741 caaaatgtta agctggctat cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa
     7801 gcaaaattag ctgaagcaaa cggatttgta aaagggacct tatgaggggg aaaagaccat
     7861 ggcaaaaaac gaactgactt tggaagaact ggcaaaacaa atttataaag gggcaggcgg
     7921 catgggcaat gtagaaagca ttgtccactg catgactcgt gtgcgtatga agatcatcaa
     7981 tgatgatctt gtaaatgtct ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga
     8041 agatgaacag cttcaagtca tcatcggacc tgggaaagtg aacaaagtag cacagcaaat
     8101 ggttgatttg gcaggcgtga aattaggaga aaaactttct tctgtaagta cagctacagc
     8161 aaattcatca gcatctggaa aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc
     8221 acaaaaatcg aaacaaaaac catctaaaat caaatcggta ttaaaagaca tttctaatat
     8281 ctttgtgcca atgattcctg catttgtcgg tactgggatc gtggcaggga ttgccgcagt
     8341 tttagcaaac ttagtcacag ccggaacact tgatgcagca acatggcaac aatatatcga
     8401 tgtgatgaac atcttgaaaa acgggatgtt tagctaccta gttatctata caggtatcaa
     8461 tgctgcacaa gtttttggtg caacaccaac tcttggtgga gttatcgggg ccgttgtgat
     8521 gctgacaggg atgaatccgg aagcaccact gaaaaactta tttactggtg aagctcttgc
     8581 agctggacaa ggtgggatcc taggtgtcat cttcgctgtt tggctattgt caatcgttga
     8641 gaaaaaactg cataaagtag tacctgatgc ggtagatatc atcgttacac caactctttc
     8701 attgattgcc atcggtctga ttgaaatttt cttcatcatg cctttagcag gtttcatttc
     8761 tgatggcatg gtcggtggga tcaactgggt acttggcgtt ggtggagcat ttgccggctt
     8821 cgttctaggc acattattct tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc
     8881 aatccatgtg caaatgattg atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat
     8941 ggctggtgca ggacaagtgg gtgcagcatt ggcattatgg attcgctgca aaaaagataa
     9001 agaattaaca gaaatgatca aaggagcttt accagtcggg atcctaggaa tcggcgaacc
     9061 attgatttac ggagtaacat tacctctagg acgcccattt attactgctt gtatcggtgg
     9121 aggtatcggc ggggcagtga tcggaatgat cggaaatgtc ggggctatcg ctattggacc
     9181 ttcaggagcc gctttgatcc cattgatctc tgacggtaaa tggttaggct acatccttgg
     9241 attattggca gcttatgctg gtggcttcgt tgctacattc ttcttcggta ttcctaaaga
     9301 ccgtttagaa aaagaagaat tagcgactga aacagccaca gtaaatgaag tgcctgaaaa
     9361 agtagtacct caaacttcag ctgaaagtgt gactatcctc tcatcagttg ctgacggaac
     9421 agttgtttct ttagataaag caagtgatcc agtttttgca caaaagatga tgggtgaagg
     9481 ctactttgta gaaccagaaa acggacatat ctattctcca gttactgggg tagtcagctc
     9541 tgtcttccca acgaaacacg ctattgggat cacaacgcca aatggtttag aaatcttgct
     9601 tcatatggga atcaacacag ttgacttagg cggtacgcca tttgatgtga aagtaacaga
     9661 agggcagcaa gtgacacctg atacgcttgt agcagaagct gacttagaag cgatcaaagc
     9721 agcaggcaaa gaaacatcca tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt
     9781 tgtcttggaa aaaacaggta aagcaaaagc aaaagcacct gtgatggatg tcaaagctta
     9841 atatgataga aacaatagaa acgaaagaag aaactgaaat ctatttttca gcttcttctt
     9901 ttttattatt tattgttttc tgctaaaatc tgataaattc gtgtcacatc agatggtgtt
     9961 ccccgtaatt cataatctgc caagaaagga aagccaaatg tttgtgcata ttgtttggct
    10021 gttagacaat actgattatt gaagttttta tttccgctac cgactacacc aagacaaagt
    10081 ttatgattgt cttcatgttc cagatattca cgcatcgttt cggtcaagat ttctgtatca
    10141 ccgttatcta aaccatttcc gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct
    10201 gtttctttcg taaaatcact attttcatgg atttctttta aagtaacttc aggattgaca
    10261 ggatcttctg catgcatttt ttcagcatat tctgctagat gtttggcaaa ggcacgcgta
    10321 tttcctgaaa tcgatatata taaaatgttc atggatcatc ctccaattct ttccttttag
    10381 tatagcatct tttttaagtt ttttggtggt gtaatttgga aagggactag gcgctttgct
    10441 tgtataatga gaaaaaagcc ggtagaatta tatttctatt aaagttttta ttaattaagt
    10501 aaaaataaaa aaggtttgtt atactttttg ataaaagcgg ttttattttg gaggatgctt
    10561 acatggatac agagcaacag aaaaaaggaa ttggtttaat tccacttgct gccttagtaa
    10621 ttggttcagc gattggaggt ggcgtatttg gtattatgac ggacatctcc ggatcggctg
    10681 gcggcccagc tattttcagt tgggtactcg taggaacagc aatgttgatg ctctccttgt
    10741 cgatcaataa tatcaataaa aaacgtccag atttaaaagg cgggatcttt agttacgcag
    10801 aagaaggttt tggccgtttc gctggtttta tcagcggttg gggttactgg ctcacatgct
    10861 ggttaggcaa cgtagctttt gcaacattgt tgatgagcgc attaggatat ttcttcccag
    10921 tatttgaagg tgggcaaaat ctggcatcga tcgtgatgag tacagtcttt ttatggggat
    10981 atgcatggct agtgaatcgg ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat
    11041 tctgtaaatt agtgccatta tttttattta ttgtagtagc tatcagcacg ttttcgatcc
    11101 aacaattcat tgataacttt tctagcgaca ttatattgaa tgcgaatgga cagccgatcc
    11161 cgttttccag tcaagtattg agttgcatca tggtgatgct ttgggtattt gttggtattg
    11221 aaggagcctc tgtcgtaggt tcacgagcaa aaaaacaatc agatgttggg aaagcaacga
    11281 ttctagggat attcggcttg attattattt acgtcttggt ttccatgctt ccatatggga
    11341 caatgacgat gaatcagctg caaacgatca aggcgcagcc tgctatgggc tatgtgtttg
    11401 aaatgatggt tggtaaatgg ggagcagtcg tgatcaacgg cggtttgatc atttctttag
    11461 ttggtgtttg gctctcatgg acgatcttac cgattgaaac aatgggaaac atggctgaag
    11521 acggactttt gcctaaagca tggggaaaac tcaacaaaaa tggggcacca acttatgcta
    11581 tcgtattgac gacgttatgt acgaatgtct ttttactttc attattattc actgacagtg
    11641 cttataactt tgcttacaca cttggtacag cagctatttt cttcacgtgg cttttcttag
    11701 gattgtacca aatgaaactt tcttatcagc ggaaagaatg ggtccaattt ttagtagggc
    11761 ttttagcaag tgcctttcaa gtatgggcta tgctagtcgt agcctttaat gaagtaatgc
    11821 ttgttcttgt tcttttcttg ccaggcattt atttttacct caaagcacgt aaggagcaaa
    11881 acgagcctgt acaacatatc cacttagata agattttact tataggtgtg acgatggtcg
    11941 gagtgattgc aatggtttta tttgccactg ggatgcttca cgtatagaag tataaaagaa
    12001 gtataaagag aggttgtgag cctgtcgttt attcaaaaac atactaggaa aggagctgtg
    12061 acacaaatgt tgtcacagct cctttttcag aataaacggt gtttaagaga cagccaatcg
    12121 gcaataaggc tgaacgtctc tttcacagcc tcttttttga tgagtcatca tacatttttt
    12181 cgcttaaata cgagatatcc taatgctaag aacaaaacga tgtaagctaa attaccgaat
    12241 agcatttgat tagtagaaag atgagtcaat tgcttcatga tctcttcgtt tccaatttgg
    12301 ttttgcagat tcaacatact gatcggattc catttgatcc actcccattt ttggatgagt
    12361 gcaataagga taccagatat gattgacgaa gcaaaataaa agacgattcc tgctgagatg
    12421 gatgcgccgg aactattgat aaagcaagca atcatcaaca ctaaacttaa aatcagccat
    12481 aaaccgatat aactacccaa agtataagtg aacaagcttt ggatcagtgt ttgaccagta
    12541 gaaaccttct ccgtgaagga aatcgaaggg aaaagaatca gtttcatcaa cacagtgaca
    12601 ataatagcta aaaggtataa ataaacagaa taaataacta atgtgatcca tttgcttact
    12661 aatatttctc ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgct
    12721 tccatcgaaa tgatcgtgct tgcagctgca atcatgataa agacaatcca agatgtggca
    12781 ctaaaaagct gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga
    12841 agtgctgttc ctatgagcaa aacaactaat agaacagata aaacagcagt tgactttttc
    12901 ttgatcaatt taaataattc ttgttttaca agcaatgtca tttttcttct tcccccttta
    12961 ctgaatccag aatttccaat agagaagatt ccaaatcatt cgtcagatgc tggacgtctg
    13021 tcacatggat attttgtgca gacaatagct ggatcacggt tcctaataat tcttcgtttt
    13081 cttttaaaag cttgatttta tcaggatctt ccaaagtata tccagcttct gtcaagatcg
    13141 ttcgagcagc tagatcattt gttgtagaga taacgatgaa atcttgcgct gtttggttca
    13201 attcagccat tgtacattgt ttgatgatct ttccttgatt gatcaggatc agctcatcga
    13261 ccattttttc aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag
    13321 ccaagtcggt aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg
    13381 gctcatctaa aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt
    13441 gtttcattcc caacgagtag gatttcgctt ttttgtgaat gtacttttcc atcttgagct
    13501 gattcacgat agtgtcgata gcagatacat cgttggtttc agagaataac ttcagatgat
    13561 catagcctgt taagaatgga tagatacccg ggtattcgat caatgctcct acttgttcca
    13621 accctttatg actagaggga gaaatctcat tttcaccaat acagattgtt ccatctgagt
    13681 aatggatcag tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta
    13741 ctaatccaac gattttgcca ggggataagc taaaactgac atcgctgagg gcttgtttgt
    13801 gaccaaagtt cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt
    13861 ctatagatag tacaagcata cacctgatga gtggagaagt cgctaggtca ttggacttat
    13921 ttcttatttt ttcataaaaa aattgaatat taaataaaaa ggcttatttt ttctgatcag
    13981 tacttattcc aagtgtctag tggccgctag taatcttgat gccaatgata ctgatgagta
    14041 gcatccccac aaagaaccaa gtggaaagat tcagcttatc atgaaacaag accacaccaa
    14101 ttatgaccga accaactgcg ccaatccctg tccagatagg ataagagaga ctcatcggca
    14161 aacttttgat tgcctttgac aaaaagtaaa aactagcaat catgccaatc actgtgtaaa
    14221 aagaataatt gattttagaa aaaccatcac tccatttcat catcgttgac cagaatactt
    14281 ccaaaattcc tgctatgatt aattccatcc atgccatact tacattcctc cttaactata
    14341 aaaaaataca caaaaagagg aaaatcatcc cttcaaagac ctaatggaat gatcatcctc
    14401 tttatcctac ccggtggcag attgtatatc tttttctgta gtatagcaca cctatccaga
    14461 tatcggcaat ctcatacttt ttagtaaagt gtaccaagca ctgacggata tatcgttagc
    14521 ggaaaagaag atgaatgctt tgcaatttca tagaaagaag ttgttattta ttaatcattt
    14581 aaaaaaaaca gtaactagac cttatttcta tatattatcg gg
//
LOCUS       DAYYJC010000276        12752 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.076, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000276 DAYYJC010000000
VERSION     DAYYJC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.076"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..523
                     /gene="dfrG"
                     /locus_tag="ACHA9N_002954"
     CDS             26..523
                     /gene="dfrG"
                     /locus_tag="ACHA9N_002954"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9086319.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(595..2547)
                     /locus_tag="ACHA9N_002955"
     CDS             complement(595..2547)
                     /locus_tag="ACHA9N_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9086320.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2554..2790)
                     /locus_tag="ACHA9N_002956"
     CDS             complement(2554..2790)
                     /locus_tag="ACHA9N_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086321.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3028..3828)
                     /locus_tag="ACHA9N_002957"
                     /pseudo
     CDS             complement(3028..3828)
                     /locus_tag="ACHA9N_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(3844..3891)
                     /locus_tag="ACHA9N_002958"
                     /pseudo
     CDS             complement(3844..3891)
                     /locus_tag="ACHA9N_002958"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4205..5134)
                     /locus_tag="ACHA9N_002959"
     CDS             complement(4205..5134)
                     /locus_tag="ACHA9N_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9086322.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5151..6173)
                     /locus_tag="ACHA9N_002960"
     CDS             complement(5151..6173)
                     /locus_tag="ACHA9N_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFM9086323.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(6170..8197)
                     /locus_tag="ACHA9N_002961"
     CDS             complement(6170..8197)
                     /locus_tag="ACHA9N_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9086324.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(8194..10647)
                     /locus_tag="ACHA9N_002962"
     CDS             complement(8194..10647)
                     /locus_tag="ACHA9N_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9086325.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(10631..11026)
                     /locus_tag="ACHA9N_002963"
     CDS             complement(10631..11026)
                     /locus_tag="ACHA9N_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9086326.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(11098..11736)
                     /locus_tag="ACHA9N_002964"
     CDS             complement(11098..11736)
                     /locus_tag="ACHA9N_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFM9086327.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(11792..12292)
                     /locus_tag="ACHA9N_002965"
     CDS             complement(11792..12292)
                     /locus_tag="ACHA9N_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9086328.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            complement(12353..>12752)
                     /locus_tag="ACHA9N_002966"
     CDS             complement(12353..>12752)
                     /locus_tag="ACHA9N_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFM9086329.1"
                     /translation="SDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEI
                     TVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHP
                     NTNIIGKREAKLIIGISSTVTDYISGYLDK"
BASE COUNT         3764 a         2485 c         2118 g         4385 t
ORIGIN      
        1 attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa
       61 tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt
      121 taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg
      181 aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa
      241 tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga
      301 aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat
      361 gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta
      421 tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa
      481 ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa
      541 gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt tttgtcattt
      601 gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc tttgagcagg
      661 tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt aagttcttag
      721 aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat agctcttctt
      781 atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc ttatctgttg
      841 aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga tttcgtttgg
      901 ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat ccttagtggc
      961 taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat ctttactaat
     1021 agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag atgccaaagt
     1081 ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt ctgcttttgc
     1141 ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt ggcaaataaa
     1201 atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg cgtttctaat
     1261 agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt gtttaattaa
     1321 ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga caaatctact
     1381 cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc tatcgttagc
     1441 ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac taaaagtatt
     1501 gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg aaatattcca
     1561 atcataggca acatcagaac ggaacacata tcttgataag acctcagcag atacaactat
     1621 atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt gtcctttctt
     1681 acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct cgttaaattt
     1741 ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac gcctatacag
     1801 ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac aagtacgact
     1861 gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt tcttttgttg
     1921 gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct tacacgcctt
     1981 acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt gcttcggctc
     2041 attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat aatctggctc
     2101 aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa cagaccaatt
     2161 agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat tgtcattgca
     2221 gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc gacttggttt
     2281 cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa acggattgat
     2341 gcagtaatta tattgaatgg gatattcaga accgttccat ttaaaagacg ttggcaagaa
     2401 caacattttg ttataccttg ttgcaaatcg ttttggagtt aataaatcat attgtttagc
     2461 cctatctttt aattcttcgt ttgaaatcgg aattttaaca tctacaattt catcttcctt
     2521 cgtggctaat cttttaagct tggtcattgt ctatcaaccc actctatctt tgatacccat
     2581 tttttcaaca atccgcttat tacttcgctt gttggcaatc cattcaataa acttcttatc
     2641 gtctaaaatg ttcaataaga attcatccac tgcttcgctt tcagtacgct ctgcatattc
     2701 agcgtattgc ttaactatca cccttacttg ctctgatatt tcccaatcca cagacttagc
     2761 gttcttattt tttggctcaa gaaattgcat aggaaaatgc accccttttt gcttagtaaa
     2821 ttgtaaccat tttaacacac caatttgcat tcctacaata attatttcac gttcgcattt
     2881 ttatatacat tttaacttac attttgtggt gtaaattaac aacaaacgtt gatttaacaa
     2941 taaaaaacga gtatcaaatt cagttgcaat ttgatactca tttaggttta cattttcact
     3001 ttttgtactc caaaactgaa ctacttaata tgcaagacgt tgtctttttt ttgtatattc
     3061 aattttgaaa acatttccgc aaagttcaga cggacctcga tgtgttgatg aataaaattt
     3121 attagtaata acttctataa ggttatcaat ccctatatta ctttttgcac ttccatgata
     3181 aagagggaac agagaacaat tctgaaatct tatgctttcc tcttgttcga gttccaatgc
     3241 ttctaatgat ttaccggaca tatatttctc taaaaggtca tcgtttccct ctattaccgt
     3301 atcccattgt tcagattcgg taaagttcgt cacacacata ttaggataca gttctacctt
     3361 ctgtttgatt acaatttcgg cagaaagttt ctctttaata tcctgataaa ccgttgataa
     3421 atcaattcca ttttggtcaa tcttattgat aaaaaagatt gtgggaatcc ccattttcct
     3481 aagtgcatga aataatatac gagtttgtgc ttgtacgcca tcttttgcag aaatcagtag
     3541 aattgcccca tctaaaactg ataatgaacg atatacttct gctaagaaat ccatatgtcc
     3601 tggcgtgtct atgatgttca ccttcgtatt ttcccactga aaagaggtta ttcctgtctg
     3661 aattgtaatt cctctctgac gttctaaaag cgtattatcc gtcctcgttg tacctttgtc
     3721 cacgcttcct aattctgtaa tcgctccact gttatataat aagctttctg ttaaggtagt
     3781 ttttcctgca tcaacatgag ctaaaactcc aatattaata attttcatgt gattttcctc
     3841 cattcaaaaa cccaaaaggg cataaaaatc ccagtgataa atacttttat cactgggatt
     3901 tttatgcata accataggta tacaaagcat acagatattc tctggatact ttagaatcac
     3961 atgataaagg tattcttaaa ctgggtacaa aaaactaagc cctcctaaaa aaggacatct
     4021 aattatttgt tcccgctatc aaattgacag tttatttaag aataccttgc cgcatattta
     4081 ttaactcctt ttaaatagtc acttaaataa tagcacgtaa gagcatattt gtaaaggaat
     4141 ctccaatttt ttatcaaaaa gagtacatga ttacaaagta tctgtaatca tgtaccaata
     4201 tttgttattt tacaatcttc caattatctt gtttttctaa tatcaactca aattgcgaga
     4261 tttgggttgc ctttgtttct tgatctaaat atttcacagc gacattcacg attacttggt
     4321 tatcttttct ggtaaagata ggattcacca attcctcgaa aacatattcc ttgttaatca
     4381 taggtaacac atgattactt acatagtaag tcaattcttt ttctgtggcg gtaggataga
     4441 gctggaagaa cgtttctaaa aagctagtga tttcgtccat cgtttctgta tctactgtat
     4501 ggtcactttc tagctgtttg ggttggtagt ccgatttttg aggtttctta ctcattgtcg
     4561 gatttttgat aataaccata ttatctgatt catctatatg gacaaccaca tgaaagctag
     4621 acgatatagt ttccttgtct ttgtcttcgg taataacttg ttcaacagaa aacagtacct
     4681 caaaggtgtt ctcattcact tgagaaacct gccacacctg tatatcgttc acactagaac
     4741 tagtaggaat atccttacga atcatttctt catttaatac ctgcaattct tccgtcaaat
     4801 aatgggtcaa tttttcattt cgtttatcaa tggcttcttg ggattgttgc cacgaaaagt
     4861 agtctttcgc aaaggatttc acaaaacttt ctacttggtt ggtatcgaca atctgctgtt
     4921 taatgatttc tgtttcacgg acagtatgag tgtcaatggc agtaaaattt ttatagattc
     4981 caaatccgac actaccaatc aataaaaccc ataaaacaaa ggttaatttc ttatgcgtgc
     5041 caactttaat catcggaact tttctttgct tgctcggttt tttagatttc tttggtttct
     5101 ccttcttttc ttttcgctcg atttttataa tcatttgatt cattcctttc ttagttatga
     5161 ataacacgtc tagccccaat cagatggtct tgccaataac gactgcttaa gtcgccataa
     5221 ccgattgggt cgcctgcatg ataaaagcga tttccctcta aatatatcgc cacatgagtc
     5281 acatatgtgc ctgcattata ggttgaatga aagaagataa ggtcgcctgc ttgggcttct
     5341 tccatagaga tttcttgcgt agcgtcatac tgatcttggg cgacacgagg gagagaaatg
     5401 ccagctttgt catacaccca ttgtattaag ccactacaat caaaagaggt ggtaggcgac
     5461 gcccctccaa agacataggg aaacccttcg tatttcaagg cttcgtccat aatgacttgc
     5521 acggtttcat catcaaattc tgttggggaa gtgtcggtta ggtattgtga aaccaactga
     5581 acataaaact gatttccata gttatatcgc caaccaccat tcacaggtat ggctatagga
     5641 ttgggatagt ctgctttttg accacccgac ttctctttag aaaactgttc ggctaattca
     5701 taggtatatt ttttcccatg actcctcaca taatttaaaa agccaccgcc ataattataa
     5761 gattggataa cagaatctat atccactcct tgttgttctg cactggttaa taattcactg
     5821 aaatatttaa ccccttgttt gatggactct tcggtactca aactgttggg cggtaaaccc
     5881 aaagattcgg aactttgcat aacatcttct gctgtaccgc ccgattccac ttgcataatg
     5941 gctaagatat aagaaacata gtcttcaatc ccatattcct taccatattt ttcaatcaac
     6001 ggacggtgtg ccaacacttc tggtgaaacg gtcacaccgc cttgtggaat agaagaatcc
     6061 ttgttgtttt cgccactgtc cgtatcatct gcaaagaaaa tgactaggaa taataagaga
     6121 aaaaagaaga gagagaagac cacactacaa atcactgtta tttttagctt catgtttgtt
     6181 gtcctcgctt atccgttctt ttatttagct tcctctgatt gaattgcttc ggtggtgtct
     6241 tttgtttttg ctcttttttc aatggtcgct gatgattttc acgtttagtg ttttgtgaca
     6301 aaacaggcgt gacggtttgc tgacgcttgc gaacatgctg ctggttattc tgaacaggag
     6361 atgatgggtt ttcttttcgt cttttttctt ttgctttatc cagctccaaa cgcttttgag
     6421 caatgttctt tcgtttttgc tcctgctggt tcatacgctg ttgtttgcgt tgagctttgg
     6481 tatctgccat agattgttta aagtctgtta cattatgagt gacattctct tttccttgat
     6541 gaagggcata tttgacatgg gttggtgtcg cctgtatctg ttgttttgcc tgctttcttt
     6601 tattgactag acgatttttc gtgtccaata catgggctgt cttttcacct aatcgttgcc
     6661 cgataccgcc ttgttgcttt ttcacattgg aaagtgctgt ttctttcttt ggtgtagtcc
     6721 gttcattttg acgagtatgg ttagctttat tggtttgatt ctgctttgtt gtcattgtct
     6781 tttgcttcgt tgaccctgct tttccacgaa aaatcttact catactgcgt tgaaatcttc
     6841 tggtttgtcg attcatcatt tgacgaggtc tgcgaaacac ttgtctgccc atgttttgag
     6901 agtcgttgct ttgtaaagtg aataggctca ttaaatcgcc cagtttaaaa taaatccctg
     6961 caaaggtcac aatttgtaaa aacataatca tgaaaaaggg agaacttgct gacagcgaat
     7021 aaagcatagt ggaaatacta aaggcaacgg taataatcaa ggtaatacca gcccttgtca
     7081 taatcacatt aaataacttg gtaatggcat gcctgcccat gccatcaaaa ctcggcaaca
     7141 tacttaatag gaaactgatt ggcaaaaaca tagcgaaaat aataaatagc acttgcgaaa
     7201 aaatcatgat tcctgttaga aggaaaacaa aaatcgagat accaatattg aatacaaaca
     7261 aaaagaagac tgttcctaat ctggaaatcg tcttcggtag ggttaaatag gtattgtcat
     7321 gatcttcaat ttcttcaatc actacatttt ctctatcttc accattgttt gtatctggac
     7381 tggtagacaa taaactttcc acacgttctt cccctaacgc ttctacatca gtggagccat
     7441 attgaagcaa tagccatggt tgctttactt gaacagagaa taagctgtca cgtatcatgt
     7501 ccacactgtc tttcccttga ctatccgtgt taggaagaac aatttttgtc ccaatattca
     7561 gactggcggt actgacatct ttggaaaaat cattgatttt cgcaatataa tcgggtgcat
     7621 acgcaataaa ggaagccgat agaataaaca ccacgacaaa gtttaaaaca gctcgtatca
     7681 ctttcgtcgt ttctcgctta accaatcctg tataggcaac atagattcca acaatgagaa
     7741 taaaaagaag gaggaaaccg acataaaacc ctgaactgga aaagccattc ggtgtaatgc
     7801 ctgcaatcgt ctgcatgttt tcgccaattg cttgggatgt ttgactaatg aaatctaagg
     7861 cataggcttc tttcaccaaa taacctgtgg cgttcgatag ataaagagaa attgtccaaa
     7921 taaaattagt aatggcatac aatccataca tcaccgattg tcctatgcca tcactccaat
     7981 tccatggaag ccagccccag ctattgtcca cataataatc aagctgatat tgattcaaac
     8041 catacttgga gtattcattg cttgtatcaa tggtttcatc aactaagcct gtcgcttgta
     8101 ctgcattgcc cagcgtgcct aataaaagta aaataacgcc cacacaaagg agcgttatcc
     8161 aactatagtg aagaaatttc ttttgttttt gtgtcattct tccacctcat ttcgcacagg
     8221 cggtctggta tcaaaggcat gaagcaactc ttcaaaaatc gggtgaaact gcataacccc
     8281 tacacgacca tagatgtctg aaactaaaca ttgtccattt tctaattcac gtaagcgttt
     8341 ctgattgttt tcatcttcct tgtccacacc aaaaaattct aaggtctttt tgatttccac
     8401 taggtcagtg gaacgaaagg caaatttcaa gccaatatta ttcttcatct tttcatctag
     8461 taaatcgtcc gcgttttgag taacgaaata gacccctgca ttcatggcac gtcctgctcg
     8521 aaccaatcgc atagatagag cttttccttg tgccacttgt aaaaaactcc aagcctcatc
     8581 aaggtcaaca attttaaaaa tggaacggtc agagtggata aagtctaaag caaaggtact
     8641 aatgacaatc agcattgcca cacttaataa ttccatggta gtgtattctt caaaagagct
     8701 attggaatcg ggtaagacca aatctgccac ttgaataata ttgagttgtt tttccaagct
     8761 aatggattgg gtgacactgc catcactgaa taacagatgg gcaaaatcat aatcgacaaa
     8821 gctttcgata tggtcagcaa tactattgct gatgggcgta tcttcttttc gcaattcatc
     8881 aatcaccaat aacaaccctc gttgtttgct ttgagttaca ctccgaatag ctttacgcag
     8941 gacaggaaac ttctcgctat ctcggctgga aatacctgtt aagaacgtga gaatatcaat
     9001 ggcaaggctt tctgaatctt tcgggagttt taaaatcaca taagggtcaa gtaacccttt
     9061 attgtcgttc tcactcgtga gattaacaat attgatttca tgggcaatat caggtaaggt
     9121 ttctttccat tcaccacgtt cagcttttgg gtcaacaatg gttgcctgtc cgccaaagag
     9181 tactgaataa tagaccagca tattgttaga gaatgatttt ccgccaccta atgaaccaat
     9241 aaaagcggac gctaatgcat ttgttacaga gcctttaatt ccttgacttg caaggctagg
     9301 ttggagataa atatttctgc ctgtatcgag attgtaaccg acgtaaatac cagaattttc
     9361 accaatcatt tgtgtagcac cgaaaccaag accagccaaa aaatccgacg tgacatattg
     9421 aatatagtca ttgatatatc gcttgcttga gggaataaat tcattatgca agcctagcat
     9481 atcgccaaaa ggtcgtacta atttgataga cagatcatca taaaaatctt ttacttcatc
     9541 acaacgttgt ttcagctcgt ccaaattctt tgctgatacc cgtaccacat agcttagttt
     9601 gtacatagat tctttggttt ggtctaaatt actttctaat tcatcgacag attctaaagc
     9661 gtccaccaca ttagtactgg tttcgttgtc gctttcccat gcgtggctat ctaaatcttt
     9721 cagttctttc tttttattgc gaacggtaga taaggctttt ttattggcga caatttcaac
     9781 attcatactg gtatcaacgg ggaaagtaaa ctgctgttgc tggtaataaa aaatttcact
     9841 attagggaaa tccaattcac caacaatact attaattgta aaatacgcca cataagtagt
     9901 ggtatcttcc tgttcaatcc gtaaatagcg ttgattttct tcgattaaac agcgagtggg
     9961 tttgagaata tcgtatttct ttaccaacac ttttttatct tcgtatcgtt tcggtaaatg
    10021 gtagtcgtag tcatcaaaag ggacacctgt ttgcccgtaa agatgttcaa taatataacc
    10081 aaaatcatct ttctccaatt tacgaatctg aaagcgtctg gataatttac tttctaacaa
    10141 ctgttccatc ttagaaaagc gttcgatttc cgccataggc atagaaacaa aatcgcccat
    10201 taatttgtga ttcacaccat gaaagaattc cgcaaatgct ttttgaattt ctttcccaac
    10261 attttttacg gatacttcct gttcattgag gtagagttta aaaccaataa aaaagcgata
    10321 gtctacttga ttgtcaccaa tcatagaaat taacgcttcg gtctgttcat ctattttttc
    10381 gtaagctatc tcttttaatc gtccagtgat ttcttttttc gattgttcct gtgtggctcg
    10441 aatacttgat tcggttgcta gttgcagagc atgaatcttg ccatcacgat tttgggcaat
    10501 caattgacag aaattatcat gaacttgaat tttttcatcg gaacttaaaa aactgtaatt
    10561 atagggaatt aattcataat aagcgaatac ttcgccatca tgattaaata cgagattatc
    10621 ttccatatat ttaatcggat atgccatcta gttgttcact ccttacaatc gtaatcgatt
    10681 cattcattgt ttcttttttt aatcgtattt gtttgccagc gtaggttact ttagggcgta
    10741 gagcataggt aatgatagac tttagaaatc catagggttt ctttccgtca aaggtctttt
    10801 ggcacatgaa cccagtgaga acaatgggaa tccctaagta tttgagtagt gctccgtcaa
    10861 tcagagaaag tggtggtaaa ctgccaaaga gtatgacgac aaacaaagaa acgataaacc
    10921 atgtcatttg cgtaaaggta attgggaaag ggagttgtaa atcattaatc gcataaatca
    10981 ctttttctac tgaccaaata ctggtgtaac ttcttatttt tttcatgtct tcacgtcctt
    11041 tccgtaaaaa tagaaaaaga acaatcaaaa taggtgattg ctctttcaat gctttaacta
    11101 ttcaatttta ttttttgtcc attttgcaat aataccttca tcagtttctt catcatctgg
    11161 attcattgaa tatctgaaac ttaaattggt atctgaccct aatcgttcat ataagctggg
    11221 tctatacaat ctcgttttat tgtcattaaa aggacttcgt tttcccagct caccatttcc
    11281 ctttaaagga tttcttctaa ctgaaaaaca atattttttt ctgggatctt ctgaatcact
    11341 ttgacttaat gagaaacaca tggcttcttt ttggtcttca tttttcttct cactaacttc
    11401 tgtcggaata aatcgagcaa gagtttggct aaattgaaat aaaacatctc ccaagttact
    11461 actgtattcg ataccaaaat attctcttat tgttttcgtg tctgtatata attttaccga
    11521 attagcatag acagctagca tatctgaaaa atcgttttcc tttaaaaatt ctagttttaa
    11581 cagagcatat tctggaactt tttcctcttt ttcaaatagc ttaacaagta caatataatt
    11641 ttgctgtttg taacgaaagt aaaaggaatc tatcacccag cctttatctt tcatgtctag
    11701 ttttaatagt ttaagatttt caaatacatt taccataatt tatacctctc cgtgctattt
    11761 attaatttaa ttataacacg gattgttaaa cttactgaca atattcaaat accccatgag
    11821 aggtaactag gtaattaccc tctatttcca tatcacgtcc caaagattgg tagtcaatat
    11881 aattttgtaa attggaaggg acttcaccaa gctgtcctgt ttcttccacg taataacggg
    11941 cgacatcttc catcgaatca caatcactat aacaatgaat atcctctttg ttttccagta
    12001 attcttccaa gctactaaac cacatgcctt gaatttcttt gagttcatta taaataggtg
    12061 taccttcgat ttcttgaatg gcttcacata gacggttgat ttcactaatc ggcgtgtatt
    12121 cgtccacatc aaaaggaagc tcaaaatcat gtattgcata ttcttcgtag tcctcattta
    12181 aaccgattct ttctgccatt tcttcctcat caattggtgg agtaaaccat gctccgacta
    12241 attcaccttc attataccgt cctaaatttg ccacatagac tttcatttcc aattcttatc
    12301 acatcctttt tataaaaata gtcataaaaa aagtagcaat cagttaatga cgctacttat
    12361 caaggtatcc gctaatataa tctgtaactg tacttgaaat acctattatt agttttgctt
    12421 ctctctttcc aataatattt gtattagggt gtgcaagtga atgcctattt cttgcttcat
    12481 taagagagct aatcattcca tttgatgacc ttatcgttgt cttcactata tcagcaagtt
    12541 cagtaggttg aatttctttt tcaattaatt gttgtatcct actatataat ttagatagat
    12601 tttcgtctct aggaactgta atttcatatt tttttagtat ttcaattaaa tatccatgaa
    12661 aagcagtgtg aactctgtca aaggcacgtt gatattcacc attactcata aaagactcag
    12721 cgtcttcaat agcttgtgaa accacatcac ta
//
LOCUS       DAYYJC010000277         6419 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.117, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000277 DAYYJC010000000
VERSION     DAYYJC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.117"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..326
                     /locus_tag="ACHA9N_002967"
     CDS             <1..326
                     /locus_tag="ACHA9N_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFM9086330.1"
                     /translation="SKKTREKVDKAISKFSIEGKVINFNSIAKEANVSKSWLYKEHDI
                     RQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVMELEKENKKLQNQIQKLYGD
                     LYNKE"
     gene            480..1262
                     /locus_tag="ACHA9N_002968"
     CDS             480..1262
                     /locus_tag="ACHA9N_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFM9086331.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1388..2119)
                     /gene="erm(A)"
                     /locus_tag="ACHA9N_002969"
     CDS             complement(1388..2119)
                     /gene="erm(A)"
                     /locus_tag="ACHA9N_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFM9086332.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2177..2236)
                     /locus_tag="ACHA9N_002970"
     CDS             complement(2177..2236)
                     /locus_tag="ACHA9N_002970"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFM9086333.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2628..3290
                     /locus_tag="ACHA9N_002971"
     CDS             2628..3290
                     /locus_tag="ACHA9N_002971"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9086334.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3525..3668
                     /locus_tag="ACHA9N_002972"
                     /pseudo
     CDS             3525..3668
                     /locus_tag="ACHA9N_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            3818..4018
                     /locus_tag="ACHA9N_002973"
     CDS             3818..4018
                     /locus_tag="ACHA9N_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9086335.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4272..5951)
                     /locus_tag="ACHA9N_002974"
     CDS             complement(4272..5951)
                     /locus_tag="ACHA9N_002974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9086336.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(5953..6165)
                     /locus_tag="ACHA9N_002975"
     CDS             complement(5953..6165)
                     /locus_tag="ACHA9N_002975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9086337.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
BASE COUNT         2069 a         1068 c         1218 g         2064 t
ORIGIN      
        1 aatcaaaaaa gacaagggaa aaagtagaca aagcgatttc taaattttcg attgaaggta
       61 aagttattaa ttttaattca atagcaaagg aagctaatgt ttctaaatca tggctttata
      121 aggaacacga tattaggcaa agaatcgaat cccttcgtga gcgtcaaata acagcaaatg
      181 tagtctcaaa acccaagaaa agttctcgtt cggaggaaat ccttattaaa accttaaaaa
      241 gaagagtaat ggaattagaa aaagaaaata aaaaattaca gaaccaaatt caaaaattat
      301 atggagatct gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta
      361 cttaaacgct aacggtcagc tttattgaac agtcatttag gtatatgtcc aatctagggt
      421 aagtaaattg agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca
      481 tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa
      541 aagatttatt tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg
      601 gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat
      661 taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg
      721 attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat
      781 atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac
      841 aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga
      901 atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag
      961 atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg
     1021 agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta
     1081 cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt
     1141 tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat
     1201 attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt
     1261 aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt attgataaat
     1321 attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta
     1381 ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata
     1441 gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga
     1501 aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt
     1561 ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg
     1621 gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca
     1681 ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa
     1741 cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac
     1801 tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt
     1861 ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca
     1921 cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct
     1981 ctttggtaaa atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat
     2041 tcgtgtgatt caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt
     2101 tagggttttt ctggttcatt ataaccttct ccttactggt tataatgaac tgacattaag
     2161 cgttcattat aaccaattaa ttttgatttg gttgataacg aactttatta ataacaaata
     2221 tagaaaaagt acccatactt ttatctcctt tccatttttt tcattatgaa tgagataaag
     2281 taatttctac tactgcgata ctggtgcaca taattgaaac ctccttatgt aactgaatat
     2341 gattgtatca tcttttcatt ttagaataaa cctttatatg taattttgca cttgacactt
     2401 cagatggacg ttttcttcaa aataatatgt tttgaaactt gataagagaa gccactgtct
     2461 ttactaaaaa gtaaaggctg cgataaagga cagtgacttt aataaagttg cactgttgta
     2521 ttgtctttgg ataaaattgt ctaaagggaa gttttatccc caacgaaaaa acatctaaag
     2581 tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg gatctttttg aatacaatag
     2641 aatcacagtt tgataaggtt gcagaagatt acgatttcgt gaatgagctt ttgaatgatt
     2701 attcattctt tgtgtctaat atgtctccaa agaaaggcag agcattagat atcggatgtg
     2761 gctcgggttt gttagtggag aaattagcaa gttattatga tgaagtggta gggattgata
     2821 tttctaatca aatgctcgat cttgccaaat ctaaacgtca actaacaaat acggtctatc
     2881 tgaatatgaa tgcagaacaa cttaatttta atgagaagtt tgattttatt gtaagccgaa
     2941 caacctttca tcatttggat gatatagcca gcgtaataca gcaaatgaag gaactgttga
     3001 atgaggaagg aagaatagtt attcttgata atgtatctga agtcgagaca ccgcctacct
     3061 atgtttataa attaggggca atccaagagt ttttaccgca ttgtttcaaa tttggaataa
     3121 aaaatgcgat tagaatttat aatcacaaca catctaaatc atggcttgag catttagctt
     3181 cagataagta cctatctgaa caaaattatt acgatttata tgaaaagttg ttgcctggat
     3241 gccaattcca taaaatgggt tgggcaatgg gggttgtctg gacaaaataa cttaaagctg
     3301 tggctttgaa aagttgaatt tgtgattgtt ttcagagcta tcgggtgctt tagtataaat
     3361 aacaatgaat aaaaaatcag cctgtatctt atgttactat taagtaattt aagatacagg
     3421 ttttactatt tttgaaataa gtaccttcca cttcaaatac atgtattcaa agtgaaagta
     3481 ttgatttaaa tgattctttt gtaatttact cttgataata tgaaattcca tcgcagcagg
     3541 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
     3601 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
     3661 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
     3721 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
     3781 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
     3841 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
     3901 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
     3961 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
     4021 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
     4081 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
     4141 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
     4201 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
     4261 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
     4321 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
     4381 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
     4441 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
     4501 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
     4561 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
     4621 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
     4681 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
     4741 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
     4801 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
     4861 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
     4921 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
     4981 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
     5041 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
     5101 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
     5161 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
     5221 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
     5281 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
     5341 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
     5401 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
     5461 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
     5521 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
     5581 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
     5641 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
     5701 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
     5761 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
     5821 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
     5881 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
     5941 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
     6001 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
     6061 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
     6121 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
     6181 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
     6241 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
     6301 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
     6361 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagat
//
LOCUS       DAYYJC010000278         5613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.129, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000278 DAYYJC010000000
VERSION     DAYYJC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.129"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1026)
                     /locus_tag="ACHA9N_002976"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="ACHA9N_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA9N_002977"
     CDS             1178..1873
                     /gene="vanR-A"
                     /locus_tag="ACHA9N_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFM9086338.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="ACHA9N_002978"
     CDS             1851..3005
                     /locus_tag="ACHA9N_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFM9086339.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="ACHA9N_002979"
     CDS             3220..4188
                     /locus_tag="ACHA9N_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFM9086340.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA9N_002980"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="ACHA9N_002980"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFM9086341.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="ACHA9N_002981"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="ACHA9N_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAYYJC010000279         4299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000279 DAYYJC010000000
VERSION     DAYYJC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.153"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            249..1727
                     /gene="msr(C)"
                     /locus_tag="ACHA9N_002982"
     CDS             249..1727
                     /gene="msr(C)"
                     /locus_tag="ACHA9N_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9086342.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1774..2220
                     /locus_tag="ACHA9N_002983"
     CDS             1774..2220
                     /locus_tag="ACHA9N_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9086343.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2419..2934)
                     /locus_tag="ACHA9N_002984"
     CDS             complement(2419..2934)
                     /locus_tag="ACHA9N_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9086344.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3051..4253
                     /locus_tag="ACHA9N_002985"
     CDS             3051..4253
                     /locus_tag="ACHA9N_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9086345.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1290 a          723 c          930 g         1356 t
ORIGIN      
        1 tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg
       61 gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg
      121 gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca
      181 aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg
      241 taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc
      301 agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag
      361 gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg
      421 atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg
      481 ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa
      541 cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt
      601 cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta
      661 ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg
      721 atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc
      781 gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc
      841 agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag
      901 aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa
      961 tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa
     1021 agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc
     1081 agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca
     1141 tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt
     1201 ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa
     1261 ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct
     1321 ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg
     1381 tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt
     1441 ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc
     1501 tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta
     1561 attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa
     1621 cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg
     1681 aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg
     1741 attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata
     1801 atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg
     1861 ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg
     1921 gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta
     1981 ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta
     2041 ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa
     2101 ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag
     2161 ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga
     2221 tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc
     2281 agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt
     2341 cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag
     2401 aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca
     2461 ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa
     2521 ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt
     2581 gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga
     2641 aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt
     2701 gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga
     2761 gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt
     2821 atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc
     2881 agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat
     2941 cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg
     3001 catattgaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat
     3061 tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag
     3121 cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa
     3181 tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta
     3241 ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga
     3301 tcggtttgaa aaaaacattg atgacaggat tgtttatcat cgttgtattc gctggtcttg
     3361 gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg ggtgtcggga
     3421 atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt ggtaacacag
     3481 aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt ggccctctag
     3541 tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta gcatttttga
     3601 tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa ccaaagagaa
     3661 aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt tcagttggac
     3721 taatcgcatt gttatataat ttcggattct ttactttact tgcgtatgcg ccatttttat
     3781 tgattggcta ttctgaatta gaagtcggtt ttgtattttt tggctggggt gtgcttctgg
     3841 ctattgcttc gattttcgtt gcaccggctc ttgagcggaa gtttacgact tatcggacaa
     3901 ttttagcagc atttgtctta ttcattattt gtttgctgtt attaggagct ggaacttcta
     3961 tgccagtcct tgtaccgatc agcattgtgt tagcaggatt tttccaagga attatcaata
     4021 cactgttgac gacgattgct atggaaatac caggtctgca acgaaacgtt gcttcgtcca
     4081 gctatagttt tgtccgcttt ttcggtggag ctttagcacc atttattgca ggtgtgattg
     4141 gtgaacatat tggtgaaagc tatgcgtttt attttgcagg tgccattgtt cttatcagtg
     4201 tatgttgtat catttatcac cgtaattatt ttgctacaaa tgaagaagaa taagaaatca
     4261 gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAYYJC010000280         3784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000280 DAYYJC010000000
VERSION     DAYYJC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.162"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1220
                     /gene="rlmN"
                     /locus_tag="ACHA9N_002986"
     CDS             153..1220
                     /gene="rlmN"
                     /locus_tag="ACHA9N_002986"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9086346.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1210..1416
                     /locus_tag="ACHA9N_002987"
     CDS             1210..1416
                     /locus_tag="ACHA9N_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086347.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1413..2516)
                     /locus_tag="ACHA9N_002988"
     CDS             complement(1413..2516)
                     /locus_tag="ACHA9N_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9086348.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3069..3617
                     /gene="aac(6')"
                     /locus_tag="ACHA9N_002989"
     CDS             3069..3617
                     /gene="aac(6')"
                     /locus_tag="ACHA9N_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9086349.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1234 a          641 c          760 g         1149 t
ORIGIN      
        1 ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt gtgagattaa
       61 agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg tatagtatgt
      121 agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt tatggcttga
      181 cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc cgagctgccc
      241 aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg acgaatctat
      301 cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta aaacagatgg
      361 tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct gataaaaaca
      421 tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg acgacacaag
      481 taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag aaccgtgatc
      541 tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt gatgagcgca
      601 atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca tttgataatt
      661 acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg gcaattggtg
      721 ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa ttcgctaata
      781 acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag gtgcgaacat
      841 cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct gtggatgagt
      901 atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat caagtaaatg
      961 atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa aaattagcat
     1021 atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct cgcagtccaa
     1081 aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac
     1141 gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc aagcaaatga
     1201 aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat aaaaggagca
     1261 gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat agcgaaagct
     1321 ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg
     1381 acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata tccttgctgg
     1441 ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa tttttctcgg
     1501 gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc taagattgct
     1561 tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag ttggttcttt
     1621 ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat caataaggtc
     1681 agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc tagaacagtc
     1741 gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac taaaacagaa
     1801 accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata tgagggacga
     1861 tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc aatgcacatt
     1921 tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg agcagaaaaa
     1981 gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta tttatcaggt
     2041 gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga aggaaagaag
     2101 agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac gggcgaacag
     2161 ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc aatgactttt
     2221 acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt ttggacactt
     2281 tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta ccagagactg
     2341 gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc ctttgaaagt
     2401 gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat tccatttgct
     2461 ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg actcatattc
     2521 atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga tctgactgcg
     2581 gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag gtggcatgaa
     2641 caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga
     2701 caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt ttttgtaatt
     2761 atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt gtcaattcat
     2821 aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca gagttttttc
     2881 ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct gtataatttt
     2941 tttattttat aagtaagata aataatgaaa gaatagagat actgattggt agctaaagat
     3001 ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa gaacaagggg
     3061 gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa gatcagcttt
     3121 ctgatttact gagactgact tggccggaag aatatggaga cagctcggca gaagaagtag
     3181 aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat gagttagtag
     3241 gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat ccattagttg
     3301 tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac ttagaaaaag
     3361 aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta gaccatggaa
     3421 caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct tccatccaga
     3481 accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc gtaggtgtct
     3541 taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg attattcctc
     3601 gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc aagaaataag
     3661 aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa
     3721 gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta
     3781 agaa
//
LOCUS       DAYYJC010000281         2803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.178, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000281 DAYYJC010000000
VERSION     DAYYJC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.178"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1417
                     /locus_tag="ACHA9N_002990"
                     /pseudo
     CDS             290..1417
                     /locus_tag="ACHA9N_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1515..1703
                     /locus_tag="ACHA9N_002991"
     CDS             1515..1703
                     /locus_tag="ACHA9N_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFM9086350.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1761..2114)
                     /locus_tag="ACHA9N_002992"
     CDS             complement(1761..2114)
                     /locus_tag="ACHA9N_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9086351.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2585..>2803
                     /locus_tag="ACHA9N_002993"
     CDS             2585..>2803
                     /locus_tag="ACHA9N_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9086352.1"
                     /translation="MKGDKEAHATPFHDRKGVKSMKPSSFQETIENQFDYICKQVIED
                     ERKDYFKHLDRLSAKEVSFSDIDNYLVNS"
BASE COUNT          944 a          457 c          472 g          930 t
ORIGIN      
        1 caaagaaact ttcatttata acacagcctt tttaactaat ttaccatcta atttcttatt
       61 tctatcaaaa aaccttttct cattatcctg cccgttagtt gaataagcaa ttttttctat
      121 aagttatatt atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt
      181 gcattttata aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc
      241 cattttgaag tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat
      301 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
      361 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
      421 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
      481 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
      541 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
      601 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
      661 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
      721 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
      781 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
      841 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
      901 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
      961 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag
     1021 ccctgttagt actccagcag attttcggat gcttactcct attgtactgg agcaagcctt
     1081 tagaaaagct ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca
     1141 ggaatatctt tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac
     1201 tcaactgaaa aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga
     1261 ttatcgcaat gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa
     1321 aggatatcag gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat
     1381 agataaagta agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa
     1441 tatggtttct tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa
     1501 aggaggagat tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa
     1561 caagactacg aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat
     1621 gtaagaacga aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag
     1681 acgccaagac gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt
     1741 gtttatctga attttactga ctagccttca atatttcttg cttcattgat accttttgct
     1801 agtgcttcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt
     1861 aaccttcgag tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca
     1921 tggagtaatg atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct
     1981 atatttgtta aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga
     2041 ccccgtttca ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct
     2101 tcttttttac gcatactaaa tcacctctat acattttact gttcctatat aaccataaac
     2161 aggtatagaa aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt
     2221 aatgcgatga atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt
     2281 ataaaattta gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag
     2341 tcgccatgag aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac
     2401 ttatccacct gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag
     2461 aaaaatcaaa gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa
     2521 taaatatctt ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt
     2581 tagtatgaag ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa
     2641 aagcatgaaa ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa
     2701 acaagttatt gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa
     2761 agaagtctcg ttttctgata tagataacta ccttgtcaac tct
//
LOCUS       DAYYJC010000282         1504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000282 DAYYJC010000000
VERSION     DAYYJC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.208"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACHA9N_002994"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACHA9N_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACHA9N_002995"
     CDS             394..477
                     /locus_tag="ACHA9N_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFM9086353.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA9N_002996"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACHA9N_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9086354.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAYYJC010000283         1363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000283 DAYYJC010000000
VERSION     DAYYJC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.215"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACHA9N_002997"
     CDS             <1..1350
                     /locus_tag="ACHA9N_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFM9086355.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          573 a          107 c          230 g          453 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tat
//
LOCUS       DAYYJC010000284         1350 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.219, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000284 DAYYJC010000000
VERSION     DAYYJC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.219"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACHA9N_002998"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACHA9N_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACHA9N_002999"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACHA9N_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFM9086356.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAYYJC010000285         1298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5848
            SAMEA115707991-rid22596013.guided.222, whole genome shotgun
            sequence.
ACCESSION   DAYYJC010000285 DAYYJC010000000
VERSION     DAYYJC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707991
            Sequence Read Archive: ERR13197716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290194.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290194.1
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:50:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,002
            CDSs (total)                      :: 2,941
            Genes (coding)                    :: 2,856
            CDSs (with protein)               :: 2,856
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 29 of 85
            Pseudo Genes (incomplete)         :: 47 of 85
            Pseudo Genes (internal stop)      :: 22 of 85
            Pseudo Genes (multiple problems)  :: 10 of 85
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707991-rid22596013.guided.222"
                     /isolate="VRE5848"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..215)
                     /locus_tag="ACHA9N_003000"
     CDS             complement(<1..215)
                     /locus_tag="ACHA9N_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086357.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELF"
     gene            308..657
                     /locus_tag="ACHA9N_003001"
                     /pseudo
     CDS             308..657
                     /locus_tag="ACHA9N_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(929..1219)
                     /locus_tag="ACHA9N_003002"
     CDS             complement(929..1219)
                     /locus_tag="ACHA9N_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9086358.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          401 a          262 c          180 g          455 t
ORIGIN      
        1 aagaatagtt ctataataat tcttgaactc catgtcttat aatgttctat cgaagcaccc
       61 aaatagccaa gttcatctaa tctacttgca taaactacgt catccgctct catagtcggc
      121 atatacatat taaagagtaa aaagataccc caaatcccta ttagtaacaa gatcagttct
      181 ttgttattcg ttatttttct attatgtttg ttcattttta tactccttct tatatttcct
      241 aatagagtat aacatagctt taagcattac ttaaagataa cttaaaagtt atcttttaaa
      301 tacttaattg ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa
      361 ttttcattat atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag
      421 aattcgtcga ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa
      481 gatatagatt cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt
      541 tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc
      601 gagttacata ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac
      661 tagtaatgac tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg
      721 gcttcgaatt gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta
      781 gtgattcatc acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc
      841 gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta
      901 ggacattttt gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt
      961 ttcttgggta tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat
     1021 aggatctcac aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc
     1081 cttcgtctac ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat
     1141 ttaaactgaa acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg
     1201 ttccatattt tttctccata accttcacct caataccatt atacgaacat acattccctt
     1261 tgtaaagaag aaactctgtt ttatcttttt gacttttt
//
